CN114948940B - Natural product monomer composition with hair blackening and strengthening functions and application thereof - Google Patents
Natural product monomer composition with hair blackening and strengthening functions and application thereof Download PDFInfo
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Abstract
The invention belongs to the field of medical cosmetics, and in particular relates to a natural product monomer composition with hair blacking and hair strengthening functions and application thereof. The natural product monomer composition comprises pinoresinol and quercetin, wherein the proportion of pinoresinol and quercetin in the composition is 10 in parts by weight: 1-1:10. studies have shown that the composition significantly enhances tyrosinase activity and promotes telogen hair follicles to enter the regeneration cycle, promoting hair growth. The composition has the effects of blackening hair, strengthening hair and the like, has the advantages of simple formula, definite active ingredients, safe use, no adverse reaction and the like, and has wide market prospect.
Description
Technical Field
The invention belongs to the field of medical cosmetics, and in particular relates to a natural product monomer composition with hair blacking and hair strengthening functions and application thereof.
Background
The traditional Chinese medicine considers that the hair is the blood and the kidney stores essence, the hair is in the hair, the growth of the hair depends on the nutrition of essence and blood, the dense black and bright hair represents health, and the good image can be given to people. However, in recent years, there are various hair problems such as endocrine imbalance, vigorous secretion of hair grease, hair loss, and white hair, which are common to modern people due to environmental pollution and increased pressure of life. Hair loss, white hair and the like can cause people to have bad psychological effects such as spelt, anxiety and the like, and serious people even influence social activities, so that modern young people generally pay attention to hair care.
White hair is generally classified into premature gray hair, commonly referred to as "premature gray hair", and senile gray hair. Premature grey hair often occurs in young or children, mostly belonging to autosomal dominant inheritance. Senile white hair is more common, and the progressive loss of tyrosinase in human hair generally occurs from the age of 35 years, and is specifically shown by the reduction of melanin generated by melanocytes in hair papillae, so that pigment in hair shafts disappears, white hair begins to appear, white hair increases after 60 years, the number of melanocytes in gray hair is normal, cells can contain vacuoles, and normal-appearance melanosomes are contained in the cells, but melanin is rarely contained in the cells. The number of melanocytes in white hair is rare or missing, the melanin granules are also obviously reduced, and the melanocytes and melanin granules are also rarely arranged in hair bulb papilla. Although senile poliosis belongs to normal physiological phenomena, in recent years, the age of the senile poliosis is greatly advanced, the degree is increased, and the speed is increased, which is obviously not a normal state.
Studies have shown that tyrosinase is a key enzyme in melanin synthesis, and that the reduced and lost function of tyrosinase elution in melanocytes of skin and hair follicles causes the reduction or loss of skin melanin, thereby causing corresponding diseases such as dry hair, no color, easy breakage, etc. The existing methods for solving the problems of white hair, alopecia, few hair and the like mainly comprise dietary conditioning, oral administration of traditional Chinese medicines and direct use of hair dyes. However, meal conditioning, while safe, is not as effective. The oral administration of the traditional Chinese medicine may have certain toxic and side effects after long-term use. The hair dye can treat symptoms and root causes, and more importantly, the hair dye can also cause allergy of human bodies and influence the environment.
Along with the discovery of social economy, the natural green component products are favored by more and more consumers, and the traditional medicine is profound in combination with the Chinese herbal medicine resources in China, so that the natural green component product has great advantages for developing herbal hair blackening and strengthening products. The theory of traditional Chinese medicine considers that the external treatment is the internal treatment, and the external treatment is the internal treatment, so the traditional Chinese medicine formulas for oral administration can be externally used. The traditional Chinese medicine considers that the formation of white hair and alopecia is related to deficiency of liver and kidney and deficiency of blood and qi, so the traditional Chinese medicine is mainly used for treating the deficiency of liver and kidney from the aspects of nourishing blood, activating blood and tonifying liver. The research at present finds that the related Chinese herbal medicines for preventing and blackening hair and strengthening hair recorded in the medical books are mainly Chinese herbal medicines which have the functions of promoting scalp blood circulation, improving hair follicle growth and development, enhancing tyrosinase activity and increasing melanin content in hair follicles. The development of herbal hair blackening and strengthening products with safety, stability and strong effect by combining modern science with ancient Chinese medical research is favored by consumers.
The inventors of the present application have focused on research on natural products and their extract-related monomer hair-blackening and hair-strengthening effects for many years, and have unexpectedly found that combination of blueberry leaf extract, biota orientalis leaf extract, eclipta extract and peppermint leaf oil in specific proportions can produce a powerful hair-blackening and hair-strengthening effect during the research.
The invention is a further improvement on the basis, and the inventor screens out a natural product monomer composition with hair blackening and hair strengthening functions, which consists of a main active ingredient of Korean pine lactone from a cacumen biotae extract and a main active ingredient of quercetin from a blueberry leaf extract, through intensive research and compounding of natural product monomers related to hair blackening and development in the natural product extract, and the two components have obvious synergistic effect, so that a natural product monomer composition product with simpler formula, more definite effective ingredients and safety and effectiveness for hair blackening and hair strengthening.
Disclosure of Invention
In order to solve the technical problems, the invention provides a natural product monomer composition with the functions of blackening and strengthening hair, which has the effects of blackening and strengthening hair, and the like, and also has the advantages of simple formula, definite active ingredients, safe use, no adverse reaction and the like, and has wide market prospect.
Specifically, the invention is realized through the following technical schemes:
in a first aspect, the present invention provides a natural product monomer composition having hair-blacking and hair-strengthening functions, the natural product monomer composition comprising pinonolide and quercetin, wherein the ratio of pinonolide to quercetin in the composition is 10 in parts by weight: 1-1:10.
alternatively, wherein the ratio of pinoresinol to quercetin in the composition is 5:1-1:5.
alternatively, wherein the ratio of pinoresinol to quercetin in the composition is 5:1. 4:1. 3:1. 2: 1. 1: 1. 1: 2. 1: 3. 1:4 or 1:5.
preferably, wherein the ratio of pinoresinol to quercetin in the composition is 4:1.
more preferably, the composition consists of pinoresinol and quercetin.
Alternatively, in the above composition, the composition significantly enhances tyrosinase activity, and promotes telogen hair follicles to enter a regeneration cycle, promoting hair growth.
In a second aspect, the present invention provides the use of a composition as described in the first aspect above for the preparation of a hair tonic product.
Alternatively, in the above-mentioned use, the product is a topical product.
Alternatively, in the above use, the product is a pharmaceutical or cosmetic product.
Alternatively, in the above use, the external product is a gel, an ointment, a cream, a foam, a shampoo, a conditioner or a spray-type liquid preparation.
It is understood that within the scope of the present invention, the above-described technical features of the present invention and technical features specifically described below (e.g., in the examples) may be combined with each other to constitute new or preferred technical solutions. Is limited to a space and will not be described in detail herein.
Compared with the prior art, the invention has the following beneficial effects:
the invention combines the Korean pine lactone and the quercetin according to a specific proportion, can generate strong hair blackening and strengthening effects, and has obvious synergy between the two components. The natural product monomer composition has the effects of blackening hair, strengthening hair and the like, has the advantages of simple formula, definite active ingredients, safe use, no adverse reaction and the like, and has wide market prospect.
Detailed Description
The invention will be further illustrated with reference to specific examples. It should be understood that the detailed description and specific examples are intended for purposes of illustration only and are not intended to limit the scope of the invention.
The specific techniques or conditions are not identified in the examples and are described in the literature in this field or are carried out in accordance with the product specifications. The reagents or equipment used were conventional products available for purchase through regular channels, with no manufacturer noted.
The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the examples described below, unless otherwise specified, are all commercially available products.
"Oriental blueberry (Vaccinium bracteatum thunder.)" is a plant of Vaccinium genus of Ericaceae, also called Vaccinium bracteatum, wu Fanzi, and Paeonia suffruticosa, etc., and is a medicinal plant. The Chinese medicine dictionary records that the Vaccinium bracteatum leaves have the effects of replenishing vital essence and energy, strengthening bones and muscles, improving eyesight and stopping diarrhea. The Chinese medicine considers that the medical food prepared by taking the juice from the tender leaves of the blueberry has the effects of reducing weight, improving eyesight, blackening hair, retaining youthful looks and prolonging life. The whole leaves of Oriental blueberry or sawtooth, tender leaves are important medicinal materials, and contain anthocyanin, friedelane alcohol, beta-sitosterol, ursolic acid, blue melanin (quercetin as main ingredient), p-hydroxy cinnamic acid, ursolic acid, oleanolic acid, etc. The blueberry leaves have various pharmacological activities of promoting the re-synthesis of the retinol, improving blood microcirculation, resisting ulcer, inflammation, aging and the like. Chinese patent CN102000017A discloses a black hair cream of Oriental blueberry plant, and provides a plant hair cream which takes natural plant Oriental blueberry leaf extract as main coloring agent, has good black dyeing effect, and has hair care and oxidation functions. Chinese patent CN111920717a discloses a natural hair dye, its preparation method and application method. The hair dye comprises two separate preparations, wherein one preparation comprises Oriental blueberry leaf extract and the other preparation comprises ferric ion. The hair dye is suitable for blackening white hair, and the hair is pure in black color, good in color and natural.
The main source of blue melanin in Oriental blueberry leaf is Quercetin (Quercetin), and formula C 15 H 10 O 7 The method comprises the steps of carrying out a first treatment on the surface of the Molecular weight: 302.236 it has the chemical name 3,3',4',5, 7-pentahydroxy flavone. Quercetin is a flavonol compound which has various biological activities, can resist free radicals, complex or capture the free radicals and prevent lipid peroxidation of organisms; can directly inhibit tumor and effectively play the roles of preventing and resisting cancer; has strong biological activity in resisting bacteria, inflammation, allergy and diabetic complications. In addition, quercetin has effects of lowering blood pressure, enhancing capillary resistance, reducing capillary fragility, reducing blood lipid, dilating coronary artery, increasing coronary blood flow, and can be used for adjuvant treatment of coronary heart disease and hypertension. The quercetin has no toxicity, so that the quercetin has great significance for treating and preventing cancers, aging and cardiovascular diseases, and has great development value. Chinese patent CN112353819a discloses a germinal preparation for promoting hair growth or repairing hair follicle tissue, which may contain quercetin. Chinese patent CN108201513A discloses a hair composition and hair growth methodThe hair growth promoting composition contains fullerene component, and optionally quercetin.
"Platycladus orientalis leaf (Platycladi cacumen)" is the dried shoot tip and leaf of Platycladus orientalis. The Chinese medicine considers that the biota orientalis leaves have faint scent, bitter and astringent taste and cold property and enter lung, liver and spleen meridians. Has effects of cooling blood, stopping bleeding, eliminating phlegm, relieving cough, promoting hair growth, and blackening hair. Can be used for treating hematemesis, hemoptysis, hematochezia, metrorrhagia, cough due to lung heat, alopecia due to blood heat, and gray hair. Chinese patent CN112842972a discloses a hair blackening liquid containing biota orientalis extract, which can effectively activate tyrosinase activity and significantly improve melanin in nascent roots. The petroleum ether extract of biota orientalis has been reported to promote hair growth, and it was primarily determined that the main active ingredients of the active site of the traditional Chinese medicine are isopimanol and pinacolide (see, for example, miao Hui, "research on chemical composition and pharmacological activity of biota orientalis", university of Jilin's university of agriculture, university of Shuin 2019, 02).
The natural product monomer pinoresinol and quercetin used in the invention can be extracted and separated from blueberry leaves, biota orientalis leaves and other plants containing the active ingredients by adopting a conventional extraction and purification method in the field of natural pharmaceutical chemistry, and can also be purchased from commercial products.
Preparation examples:
example 1:
a natural product monomer composition having hair-blacking and hair-strengthening functions, the natural product monomer composition consisting of pinonolide and quercetin, wherein the ratio of pinonolide to quercetin in the composition is 4:1.
example 2:
a natural product monomer composition having hair-blacking and hair-strengthening functions, the natural product monomer composition consisting of pinonolide and quercetin, wherein the ratio of pinonolide and quercetin in parts by weight in the composition is 5:1.
example 3:
a natural product monomer composition having hair-blacking and hair-strengthening functions, the natural product monomer composition consisting of pinonolide and quercetin, wherein the ratio of pinonolide and quercetin in parts by weight in the composition is 3:1.
comparative example 1:
a natural product monomer Korean pine lactone.
Comparative example 2:
a natural product monomer quercetin.
Comparative example 3:
a natural product monomer composition consisting of pinoresinol and quercetin, wherein the ratio of pinoresinol to naringenin in the composition is 4:1.
in the following effect experiments, the test substances were prepared by those skilled in the art by adding a certain amount of the compositions described in examples 1 to 3 and comparative examples 1 to 3 to a suitable solvent or cosmetic base according to actual needs.
Effect example 1: tyrosinase activation assay
Preparing an L-dopa solution with the concentration of 1g/L by adopting 0.2mol/L phosphate buffer solution (pH of 6.8); preparing tyrosinase solution with concentration of 100U/mL by adopting 0.2mol/L phosphate buffer solution (pH of 6.8); preparing a liquid medicine to be tested containing the compositions of examples 1-3 or comparative examples 1-3 by using 0.2mol/L phosphoric acid buffer solution (pH is 6.8) (the drug concentration of each component in the composition is shown in Table 1); adding 40 mu L of L-dopa solution, 40 mu L of liquid medicine to be detected and/or 40 mu L of tyrosinase solution into a 96-well plate, and then reacting for 40min in a water bath at 30 ℃; testing absorbance at 490nm after the reaction is completed, repeating each group of experiments for 3 times, and taking the average value of the 3 tests of the absorbance of each group of experiments to obtain absorbance C; referring to the method, the tyrosinase solution is replaced by phosphate buffer solution, and the absorbance is measured to be D; replacing the liquid medicine to be tested with a phosphoric acid buffer solution, and testing to obtain absorbance A; and (3) replacing the liquid medicine to be tested and the tyrosinase solution with a phosphate buffer solution, and testing to obtain absorbance B.
Tyrosinase activation rate (%) = [ (C-D) - (a-B) ]/(a-B) 100%
Wherein, the higher the tyrosinase activation rate is, the stronger the melanin generation promoting capability is, namely, the better the hair blacking effect of the natural product monomer composition is.
Table 1: tyrosinase activation test results of the Natural product monomer composition of the invention
From the experimental data in table 1, it can be seen that the natural product monomer compositions of the groups 1-3 all have significant tyrosinase activation. Among them, the group 1 natural product monomer composition had the highest tyrosinase activation rate. Also, the tyrosinase activation rate of the natural product monomer compositions of examples 1-3 was significantly higher than that of the comparative examples 1-2 using only a single natural product monomer.
As shown in the experimental results of the comparative example 1 group, the tyrosinase activation rate of the pinoresinol alone was significantly lower than that of the example 1 group in which pinoresinol was used in combination with quercetin. As shown in the experimental results of the comparative example 2 group, the tyrosinase activation rate of quercetin alone was only 21.09%, which is almost equivalent to that of quercetin without tyrosinase activation, and it was reported in some documents in the prior art that quercetin may have tyrosinase inhibitory effect. Surprisingly, however, when Korean pine lactone is combined with quercetin, the tyrosinase activation rate of the composition is significantly increased, up to 195.08%.
As shown in the experimental results of comparative example 3, when the pinoresinol is used in combination with naringenin, although naringenin and quercetin belong to flavonoid natural products and have similar chemical structures, naringenin cannot enhance the tyrosinase activation rate of pinoresinol.
Effect example 2: white hair to black test
100 subjects with grey hair (number of grey hair greater than 100) were enrolled and randomized into 5 groups of 20 persons each, each half of men and women. Group 1 is a blank control group using only a general hair cream blank matrix, and groups 2 to 5 respectively using a hair cream matrix comprising the natural product monomer compositions of example 1 and comparative examples 1 to 3. In addition, any product with the effect of turning white hair into black hair is not used.
The subjects uniformly apply the composition to the scalp, hair roots to hair tips once in the morning and evening, and gently massage the composition to make the composition absorbed uniformly. The number of white hairs in the head-immobilized area of the subject was recorded once a week, and each group of subjects was continuously used for 4 weeks. The experimental results are shown in table 2 below:
table 2: effect of the Natural product monomer composition of the invention on the number of white hairs in a subject
As can be seen from the experimental data in table 2, the example 1 group of natural product monomers were able to significantly reduce the number of white hairs in the subjects. Of these, the group 1 natural product monomer compositions of example showed significantly higher reduction in white hair (42.62%) in subjects than the group 1-2 of the comparative examples, which used only a single natural product monomer.
As shown in the experimental results of the comparative example 1 group, the reduction rate of white hair in the subject was significantly lower when using the pinoresinolide alone than in the example 1 group in which the pinoresinolide was combined with quercetin.
As shown by the experimental results of the comparative example 2 group, the use of quercetin alone can also slightly reduce the subject's grey hair, unlike the results obtained in effect example 1, presumably related to the coloring effect of quercetin itself on hair.
As shown in the experimental results of comparative example 3, when the pinoresinol is used in combination with naringenin, although naringenin and quercetin belong to flavonoid natural products and have similar chemical structures, naringenin cannot enhance the reduction rate of white hair of pinoresinol to subjects.
Effect example 3: effect of the Natural product monomer composition of the invention on the Condition of mouse Hair growth
Male C57BL/6J mice of 6-8 weeks of age were randomly grouped into 5 groups of 10 mice each. Group 1 is a blank control group using only a general hair cream blank matrix, and groups 2 to 5 respectively using a hair cream matrix comprising the natural product monomer compositions of example 1 and comparative examples 1 to 3.
Each group was given to the test object after shaving (3×3 cm) on the backs of the mice for 24 hours, 1 time/day, and the test object was given continuously for 21 days, and after the end of the experiment, the experimental animals were euthanized, the newly grown hair length was measured and the test area was counted for hair follicles.
Statistical data are expressed as mean ± standard error. Statistical analysis was performed using repeated-assay analysis of variance and the differences between groups were compared using Fisher LSD test.
Experimental data are shown in table 3 below.
Table 3: effect of the Natural product monomer composition of the invention on the Hair growth Length and the number of hair follicles in mice (average.+ -. SE)
Note that: ** ，p<0.01, compared to the blank; ## ，p<0.01, compared with comparative example 1 group
As can be seen from the experimental data in table 3, the example 1 group natural product monomers were able to significantly increase the hair growth length and the number of hair follicles in mice (p < 0.01) compared to the blank group.
As shown by the experimental results of the comparative example 1 group, the hair growth length and the number of hair follicles of the mice were significantly inferior to those of the example 1 group in which the pinolactone was combined with quercetin, and the experimental results of the example 1 group were significantly different from those of the comparative example 1 group (p < 0.01).
As shown by the experimental results of the comparative example 2 group, the use of quercetin alone can only slightly increase the hair growth length and the number of hair follicles in mice.
As shown in the experimental results of comparative example 3, when the pinoresinol is used in combination with naringenin, although naringenin and quercetin belong to flavonoid natural products and have similar chemical structures, naringenin cannot enhance the promotion effect of pinoresinol on the hair growth and hair follicle number of mice.
It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (5)
1. A natural product monomer composition having hair blacking and hair tonic functions, characterized in that: the natural product monomer composition consists of pinoresinol and quercetin, wherein the ratio of pinoresinol to quercetin in parts by weight in the composition is 4:1.
2. Use of the composition of claim 1 for the preparation of hair tonic products.
3. Use according to claim 2, characterized in that: the product is an external product.
4. Use according to claim 2, characterized in that: the product is a medicament or cosmetic.
5. Use according to claim 3, characterized in that: the external product is gel, ointment, cream, foam, shampoo, hair conditioner or spray liquid preparation.
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| WO2016021872A1 (en) * | 2014-08-04 | 2016-02-11 | 두리화장품 주식회사 | External skin preparation composition containing herbal medicine extract, for preventing hair loss and promoting hair growth |
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| 侧柏叶的化学成分及药理活性研究;苗慧;中国硕士学位论文全文数据库 医药卫生科技辑(第02期);摘要，第39-40页"4.2.7 小结与讨论"部分 * |
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