CN114869929A - Traditional Chinese veterinary medicine composition for treating nodular skin disease of cattle - Google Patents
Traditional Chinese veterinary medicine composition for treating nodular skin disease of cattle Download PDFInfo
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- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A61P17/00—Drugs for dermatological disorders
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- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
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- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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Abstract
The invention discloses a composition for treating bovine sarcoidosis, which is prepared from the following raw materials in parts by weight: 3-12 parts of cocklebur fruit, 6-15 parts of common cnidium fruit, 6-20 parts of belvedere fruit and 10-22 parts of lightyellow sophora root. The composition disclosed by the invention can expel wind, remove dampness, kill insects, effectively inhibit virus growth, effectively treat bovine sarcoidosis, and simultaneously has a good broad-spectrum bactericidal effect and a good effect on killing a colony house.
Description
Technical Field
The invention particularly relates to a composition for treating bovine sarcoidosis.
Background
Bovine sarcoidosis (LSD), also known as bovine sarcoidosis or bovine sarcoidosis, is an acute, subacute infectious disease of cattle caused by bovine sarcoidosis virus (LSDV). Animal epidemic diseases listed as a must be notified by the world animal health Organization (OIE). The infection rate of the disease is 5-85%, and the fatality rate is 10%; the main clinical characteristics are that a large number of nodule-like nodules appear on the skin surface of a sick cow, the body temperature is raised to be above 40 ℃, the cow is thinned, and the milk yield is suddenly reduced by about 50%. At the same time, primary and secondary pneumonia can also be caused; lameness of infected affected livestock limbs caused by synovitis and tenosynovitis; the sick female livestock are aborted, and the aborted fetus is wrapped by nodular nodules and is complicated with endometritis; temporary or permanent sterility of the bull; the tanned hide has a pit or a hole to cause huge economic loss and has great harm to the cattle industry.
There are three main approaches to treatment of LSD: (1) isolating sick cattle in time, disinfecting a cattle bed by bleaching powder, disinfecting cattle bodies by peracetic acid, and simultaneously placing the fly killing agent in the cattle shed to well kill mosquitoes and flies. Injecting long-acting penicillin into sick cattle for 1 time per day, smearing iodophor on the skin of an affected part for 1-2 times, and washing the affected eye with ciprofloxacin solution for 2-3 times; (2) vaccinated cattle, vaccinated with a vaccinated herd, have lifelong immunity to bovine sarcoidosis, but approximately half of the animals will develop the other manifestations of vaccination. For example, the injection site is painful and swollen for a plurality of weeks, so that the yield is reduced, and about half of the animals are not subjected to any physical discomfort after inoculation; (3) the diseased cattle with the bovine sarcoidosis adopts immediate killing and cutting off the transmission path of the diseased cattle. At present, no effective direct treatment method exists, and no research report of a traditional Chinese medicine prescription for the bovine sarcoidosis exists.
The infectious diseases of animals are spread rapidly, most economic animals are cultured in a concentrated mode, once infection occurs, the infection can be spread rapidly, the environment is polluted in a large amount, the economic loss is large, the antibiotic drug resistance is enhanced and drug-resistant strains are increased by using traditional antibiotic treatment, the threat to the healthy development of animal husbandry is brought, and meanwhile, the human health is threatened through a food source approach. Therefore, an external medicine which can treat the bovine sarcoidosis and replace the traditional antibiotics is needed, and meanwhile, the external medicine has a certain promotion effect on environmental improvement, so the medicine for treating the bovine sarcoidosis has certain practical significance.
Disclosure of Invention
In order to solve the problems, the invention provides a composition for treating bovine sarcoidosis, which is prepared from the following raw materials in parts by weight:
3-12 parts of cocklebur fruit, 6-15 parts of common cnidium fruit, 6-20 parts of belvedere fruit and 10-22 parts of lightyellow sophora root.
Further, the traditional Chinese medicine is prepared from the following raw material medicines in parts by weight:
3-10 parts of cocklebur fruit, 6-10 parts of fructus cnidii, 7-16 parts of fructus kochiae and 10-22 parts of radix sophorae flavescentis.
Furthermore, the traditional Chinese medicine is prepared from the following raw materials in parts by weight: 10 parts of cocklebur fruit, 10 parts of common cnidium fruit, 10 parts of belvedere fruit and 10 parts of lightyellow sophora root.
Furthermore, the traditional Chinese medicine composition is a preparation prepared by taking medicinal powder of raw material medicines, or water or organic solvent extracts of the raw material medicines as active ingredients and adding pharmaceutically acceptable auxiliary materials.
Further, the preparation is an external preparation.
Further, the external preparation is a solution, a spray, a paste, a powder, a granule or a pill, preferably a spray.
The invention also provides a preparation method of the composition, which comprises the following steps:
(1) weighing the raw materials according to the proportion;
(2) grinding the raw materials into powder, or extracting with water or organic solvent, and adding pharmaceutically-acceptable adjuvants or auxiliary components.
Further, the cocklebur fruit and the common cnidium fruit in the raw material medicines in the step (2) are soaked in water, extracted by refluxing and filtered to obtain water extract and dregs; adding ethanol into the water extract, and standing for 6-12 h to obtain an ethanol precipitation solution; extracting the residues, Kochiae fructus and Sophorae radix with ethanol under reflux to obtain ethanol extractive solution; filtering the ethanol precipitation solution, mixing with the ethanol extractive solution, and removing impurities and precipitate.
Further, the cocklebur fruit and the common cnidium fruit in the raw material medicines in the step (2) are soaked in water for 2-4 hours, extracted for 2-4 hours in a refluxing manner, and filtered to obtain water extract and dregs; adding 85-100% ethanol into the water extract until the alcohol content is 35-70%, and standing for 6-12 h to obtain an ethanol precipitation solution; taking the medicine residues, the belvedere fruit and the radix sophorae flavescentis, and adding 35-70% ethanol for reflux extraction for 2-4 hours to obtain an ethanol extract; filtering the ethanol precipitation solution, mixing with the ethanol extractive solution, and removing impurities and precipitate.
The invention finally provides the application of the composition in preparing a medicament for treating the bovine sarcoidosis.
In the pharmaceutical composition, the fructus cnidii is a dry mature fruit of Cnidium monnieri (L.) cuss of an umbelliferae plant, is pungent, bitter and warm in taste, has the effects of eliminating dampness, dispelling wind, killing parasites, relieving itching, warming kidney and tonifying yang, and the fructus xanthil is a dry mature fruit with buds of Xanthium sibiricum patrim ex Widder of an Compositae plant, is bitter, sweet and pungent in taste, is warm in nature, and has the effects of dispersing wind cold, relieving stuffy nose and dispelling wind-damp; the fructus Kochiae is dry mature fruit of Kochia scoparia (L.) Schrad of Chenopodiaceae, is pungent, bitter and cold in taste, and has the effects of clearing heat, promoting diuresis, dispelling pathogenic wind and relieving itching; the radix Sophorae Flavescentis is dried root of Sophora flavescens Alt. of Leguminosae, and has bitter and cold properties, and has effects of clearing heat, eliminating dampness, killing parasite, and promoting urination. The common medicinal formula is used for treating human skin diseases such as acute and chronic eczema, various dermatitis, skin itch, scabies and the like, and has the effects of killing parasites, relieving itching and astringing.
Obviously, many modifications, substitutions, and variations are possible in light of the above teachings of the invention, without departing from the basic technical spirit of the invention, as defined by the following claims.
The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Detailed Description
EXAMPLE 1 preparation of a formulation of a composition of the invention
The formula is as follows: 10kg of cocklebur fruit, 10kg of common cnidium fruit, 10kg of broom cypress fruit and 10kg of lightyellow sophora root.
The preparation method comprises the following steps: weighing raw materials according to a ratio, taking cocklebur fruit and common cnidium fruit, adding 200kg of water, soaking for 2 hours, performing reflux extraction for 2 hours, filtering to obtain a water extract, adding 85% ethanol into the water extract until the alcohol content is 35%, standing overnight to obtain an ethanol precipitation solution;
adding fructus Xanthii and fructus Cnidii residues, Kochiae fructus, and radix Sophorae Flavescentis, adding 35% ethanol 200kg, reflux extracting for 2 hr to obtain ethanol extractive solution, filtering the above ethanol precipitation solution, mixing with the ethanol extractive solution, removing impurities and precipitate to obtain Chinese medicinal spray.
The using method comprises the following steps: is sprayed to the affected part uniformly in an amount to infiltrate the focus, 1-2 times per day for 3-5 days.
Example 2 preparation of a formulation of a composition of the invention
The formula is as follows: 6kg of cocklebur fruit, 7kg of common cnidium fruit, 11kg of broom cypress fruit and 16kg of lightyellow sophora root.
The preparation method comprises the following steps: weighing raw materials according to a ratio, taking the cocklebur fruit and the common cnidium fruit, adding 275kg of water, soaking for 3 hours, carrying out reflux extraction for 3 hours, filtering to obtain a water extract, adding 93% ethanol into the water extract until the alcohol content is 52%, standing overnight to obtain an alcohol precipitation solution;
adding fructus Xanthii residue and fructus Cnidii residue, Kochiae fructus, and radix Sophorae Flavescentis, adding 52% ethanol 450kg, reflux extracting for 4 hr to obtain ethanol extractive solution, filtering the above ethanol precipitation solution, mixing with the ethanol extractive solution, removing impurities and precipitate to obtain Chinese medicinal spray.
The using method comprises the following steps: is sprayed to the affected part uniformly in an amount to infiltrate the focus, 1-2 times per day for 3-5 days.
Example 3 preparation of a formulation of a composition of the invention
The formula is as follows: 9kg of cocklebur fruit, 8kg of common cnidium fruit, 16kg of belvedere fruit and 22kg of lightyellow sophora root;
the preparation method comprises the following steps: weighing raw materials according to a ratio, taking cocklebur fruit and common cnidium fruit, adding 350kg of water, soaking for 4 hours, performing reflux extraction for 4 hours, filtering to obtain a water extract, adding 100% ethanol into the water extract until the alcohol content is 70%, and standing overnight to obtain an alcohol precipitation solution;
taking cocklebur fruit dregs and cnidium fruit dregs, belvedere fruit and lightyellow sophora root, adding 700kg of 70% ethanol, carrying out reflux extraction for 6 hours to obtain an ethanol extract, filtering the ethanol precipitation solution, mixing with the ethanol extract, removing impurities and precipitates to obtain the traditional Chinese medicine spray.
The using method comprises the following steps: is sprayed to the affected part uniformly in an amount to infiltrate the focus, 1-2 times per day for 3-5 days.
Comparative example 1
The formula is as follows: 18kg of cocklebur fruit, 12kg of common cnidium fruit, 9kg of belvedere fruit and 14kg of lightyellow sophora root;
the preparation method and the using method are the same as the example 1
The advantageous effects of the present invention are described below by way of test examples.
Test example 1 animal test
1. The test method comprises the following steps: the method is characterized by collecting and treating the bovine nodular skin diseases appearing in the field, respectively using the medicine and the existing main treatment medicines or treatment methods to treat cases after random grouping, observing treatment conditions, curing periods, recurrence conditions, infection conditions and the like, and carrying out statistical analysis on the results.
2. Grouping: 84 diseased cows with bovine sarcoidosis found in the field are randomly divided into 6 groups, namely, a treatment 1 group, a treatment 2 group, a treatment 3 group, a blank group and a control group, and a comparison group, wherein the varieties, the sexes and the weight average of the diseased cows in each group have no significant difference and are comparable.
3. The treatment method comprises the following steps: treatment of 1 group, the spray prepared in example 1 was given, used according to the specified method of use, continuously used for 2 courses, and sprayed in the environment of sick cattle; treating 2 groups, applying the spray prepared in the embodiment 2, using according to the application method, continuously using for 2 treatment courses, and spraying the spray in the environment of sick cattle; treating 3 groups, applying the spray prepared in the embodiment 3, using according to the application method, continuously using for 2 courses of treatment, and spraying the spray in the environment of sick cattle; fourthly, in the control group, the sick cattle are injected with the long-acting penicillin 1 time per day, the affected eyes are washed with the ciprofloxacin solution 2 times, 5d is a treatment course, and the control group is continuously used for 2 treatment courses. Blank group, no treatment, only observation. Sixthly, the spray prepared in the comparative example 1 is given according to the use method, is continuously used for 2 courses of treatment and is sprayed in the environment of sick cattle.
4. And (3) observation of curative effect: the main observation items are: (ii) recent observations: the body temperature, feed intake and focus properties of the sick cattle are mainly observed; secondly, long-term observation: the main observation is the cure cycle, recurrence and infection.
5. The results were statistically analyzed as shown in tables 1 and 2:
TABLE 1 recent observations
TABLE 2 Long term observations
The statistical results in the table show that the spray of the invention has the cure rate of over 60 percent, wherein the spray of the embodiment 1 has the best effect, the cure rate of over 71 percent and the effective rate of over 100 percent, the cure period is only 5-6 days, the spray has good treatment effect on the bovine sarcoidosis, the treatment effect is stable, and no relapse occurs in the observation period, meanwhile, the spray of the invention has good effect on the environment when being sprayed in the treatment process, and the skin and the effluent tissue fluid of the sick cattle shed also contain a large amount of viruses and can keep the activity for a long time.
In conclusion, the invention is the external spray for treating the nodular skin disease of the cattle, and has certain killing effect on the environment.
Claims (10)
1. A traditional Chinese veterinary medicine composition for treating bovine nodular skin diseases is characterized in that: the traditional Chinese medicine is prepared from the following raw materials in parts by weight:
3-12 parts of cocklebur fruit, 6-15 parts of common cnidium fruit, 6-20 parts of belvedere fruit and 10-22 parts of lightyellow sophora root.
2. The veterinary drug composition according to claim 1, characterized in that: the traditional Chinese medicine is prepared from the following raw materials in parts by weight:
3-10 parts of cocklebur fruit, 6-10 parts of fructus cnidii, 7-16 parts of fructus kochiae and 10-22 parts of radix sophorae flavescentis.
3. The veterinary Chinese medicine composition according to claim 2, wherein: the traditional Chinese medicine is prepared from the following raw materials in parts by weight:
10 parts of cocklebur fruit, 10 parts of common cnidium fruit, 10 parts of belvedere fruit and 10 parts of lightyellow sophora root.
4. The veterinary Chinese medicine composition according to claim 1, wherein: the preparation is prepared by taking medicinal powder of raw material medicines, or water or organic solvent extracts of the raw material medicines as active ingredients and adding pharmaceutically acceptable auxiliary materials.
5. The veterinary Chinese medicine composition according to claim 4, wherein: the preparation is an external preparation.
6. The veterinary drug composition according to claim 5, wherein: the external preparation is a solution, a spray, a paste, powder, granules or pills, preferably a spray.
7. A process for the preparation of a veterinary herbal composition according to any one of claims 1 to 6, wherein: it comprises the following steps:
(1) weighing the raw material medicines according to the proportion of claim 1;
(2) grinding the raw materials into powder, or extracting with water or organic solvent, and adding pharmaceutically-acceptable adjuvants or auxiliary components.
8. The method of claim 7, wherein: soaking fructus xanthil and fructus cnidii in the raw material medicines in the step (2) by adding water, performing reflux extraction, and filtering to obtain water extract and dregs; adding ethanol into the water extract, and standing for 6-12 h to obtain an ethanol precipitation solution; extracting the residues, Kochiae fructus and Sophorae radix with ethanol under reflux to obtain ethanol extractive solution; filtering the ethanol precipitation solution, mixing with the ethanol extractive solution, and removing impurities and precipitate.
9. The method of claim 8, wherein: soaking fructus xanthil and fructus cnidii in the raw material medicines in the step (2) in water for 2-4 h, performing reflux extraction for 2-4 h, and filtering to obtain water extract and dregs; adding 85-100% ethanol into the water extract until the alcohol content is 35-70%, and standing for 6-12 h to obtain an ethanol precipitation solution; taking the medicine residues, the belvedere fruit and the radix sophorae flavescentis, and adding 35-70% ethanol for reflux extraction for 2-4 hours to obtain an ethanol extract; filtering the ethanol precipitation solution, mixing with the ethanol extractive solution, and removing impurities and precipitate.
10. Use of a composition according to any one of claims 1 to 6 in the manufacture of a medicament for the treatment of bovine sarcoidosis.
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| CN107468962A (en) * | 2017-08-08 | 2017-12-15 | 高宗旺 | It is a kind of to be used to treat external medicine composition of milk cow mange and scab and preparation method thereof |
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