CN114830868B - Method for culturing seeds of fragrant fruit trees - Google Patents
Method for culturing seeds of fragrant fruit trees Download PDFInfo
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- CN114830868B CN114830868B CN202210592227.4A CN202210592227A CN114830868B CN 114830868 B CN114830868 B CN 114830868B CN 202210592227 A CN202210592227 A CN 202210592227A CN 114830868 B CN114830868 B CN 114830868B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The invention discloses a method for culturing seeds of a fragrant fruit tree, which belongs to the technical field of fragrant fruit tree culture, and comprises the following steps of firstly carrying out ultrasonic cleaning on fragrant fruit tree seeds with full seeds and high maturity, airing and then soaking in a seed soaking agent; then, placing the treated myrtle seed in a nutrient solution to carry out magnetic heating and stirring alternately in a positive and negative direction to obtain a nutritive seed; scattering the nutritive seeds on fine sand, periodically turning over and spraying water to obtain the dewy fragrant fruit tree seeds; and (3) inoculating the chamomile tree seeds on a germination culture medium to obtain seedlings. The combined action of the steps effectively shortens the germination period of the seeds of the fragrant fruit tree and improves the germination rate of the seeds of the fragrant fruit tree.
Description
Technical Field
The invention relates to the technical field of fragrant fruit tree cultivation, in particular to a fragrant fruit tree seed cultivation method.
Background
The myrtle is a single species peculiar to China, only comprises one myrtle tree, a known natural distribution area is only in China, is mainly distributed in a subtropical Yangtze river basin and a southwest area, only a few populations are expanded to a northern slope of the Qinling mountain, namely the south-most side of a northern temperate zone, is adjacent to the subtropical area, and belongs to a national secondary protection plant. The Syzygium aromaticum tree is a deciduous tree in the genus of Syzygium of Rubiaceae, has a height of 30m, and has a grey brown bark and a flaky shape; the small branches are thick and strong with skin holes, the leaves are paper or leather, the leaves are oblong, oval or oval, the upper surface has no hair or is dredged by rough hair, the lower surface is pale, the supporting leaves are large, the triangle oval is shaped, and the conical umbelliform rosette grows; flower fragrance, nearly circular cleft, white, light red or yellowish metamorphic leafy calyx cleft, funnel-shaped corolla, white or yellow, and filigree villi. The capsule is long round oval or nearly fusiform, has a length of 3-5 cm and a diameter of 1-1.5 cm, and has no hair or short soft hair and longitudinal and fine edges; the seeds are most small and have wide wings, the flowering period is 6-8 months, and the fruit period is 8-11 months. The bark and root of the myrtle can be used as a medicine, and has the effects of neutralizing the stomach by dampness, lowering the adverse flow of qi and preventing vomiting.
The sweet trees prefer mild or cool climate and moist fertile soil, and are usually scattered in evergreen broad-leaved forests mainly comprising fagaceae or grown in evergreen deciduous broad-leaved mixed forests. The age of the first flowering of the fragrant fruit tree is larger, the age of the first flowering fragrant fruit tree observed in the field is over 20 years old, and the flowering of the fragrant fruit tree has a longer interval, usually 2-4 years; the seed of the fragrant fruit tree has long germination period, low germination rate of only 10-20%, strict germination conditions, strict requirements on temperature, illumination, humidity and the like, and the seed cannot germinate after only one year of vitality due to less nutrient components stored in the seed. Aiming at the technical problems, the invention provides a method for culturing seeds of a myrtle tree.
Disclosure of Invention
The invention provides a method for culturing the seeds of the fragrant fruit tree, which effectively shortens the germination period of the fragrant fruit tree seeds, improves the germination rate of the seeds, and increases the nutritional ingredients of the fragrant fruit tree seeds, thereby improving the life vitality of the fragrant fruit tree seeds.
The invention provides a method for culturing seeds of a myrtle tree, which is characterized by comprising the following steps of:
s1: seed pretreatment: collecting mature fragrant fruit tree seeds, ultrasonically cleaning, airing, and soaking in a seed soaking agent to obtain pretreated seeds;
s2: endowing nutrition: performing positive and negative bidirectional alternating magnetic heating stirring on the pretreated seeds in the S1 in a nutrient solution to obtain nutritive seeds;
s3: white culture: spreading fine sand on a plurality of layers of porous gauze, scattering the nutritional seeds S2 on the fine sand, turning over regularly and spraying water to obtain the lubai Xiangguo tree seeds;
s4: germination culture: and (4) inoculating the dewing myrtle tree seeds in the S3 on a germination culture medium to obtain seedlings.
Preferably, in S2, the nutrient solution is composed of the following raw materials in parts by weight: 5 to 8 parts of livestock and poultry manure, 10 to 20 parts of passion fruit residues, 10 to 15 parts of nitrogen-phosphorus-potassium compound fertilizer, 3 to 5 parts of fly ash and 500 to 700 parts of water.
Preferably, in S2, the temperature of the magnetic heating and stirring is 20-30 ℃, the stirring speed is 800-1500 r/min, and the stirring time is 1-3 h.
Preferably, in S3, the porous gauze and the fine sand are both soaked with a germination accelerating solution, and the germination accelerating solution is a cyanamide aqueous solution with a mass fraction of 0.01% -0.05%.
Preferably, in S3, the temperature of the white exposure culture is 26-30 ℃, the humidity is 75-85%, the illumination intensity is 1800-2000 Lx, and the illumination time is 5-7 h/day.
Preferably, in S4, the formula of the germination medium is 8-10 g/L agar powder, 15-20 g/L white granulated sugar, 2-4 g/L passion fruit juice, 5-8 g/L corn flour and 3-6 g/L bran 1-1.5 g/L active carbon.
Preferably, in S1, the frequency of ultrasonic cleaning is 0.5 kHz-1 kHz, and the ultrasonic cleaning time is 10-30 min.
Preferably, in S1, the seed soaking agent contains 0.05-0.25% by mass of amobam solution and 0.1-0.4% by mass of sodium chloride solution.
Preferably, the volume ratio of the ambam solution to the sodium chloride solution is 1.
Preferably, before the seeds of the fragrant fruit tree are soaked by the seed soaking agent, the seeds of the fragrant fruit tree are disinfected, and the treatment steps are as follows: soaking the fragrant fruit tree seeds subjected to ultrasonic cleaning in a procymidone solution with the mass fraction of 0.01-0.03% for 20-40 seconds, cleaning with sterile water, drying in the air, and performing radiation treatment for 10-20 min by using blue light with the wavelength of 435-450 nm.
Compared with the prior art, the invention has the beneficial effects that:
(1) Firstly, ultrasonically cleaning fragrant fruit tree seeds with full seeds and high maturity, airing and then soaking in a seed soaking agent; then, placing the treated myrtle seed in a nutrient solution to carry out magnetic heating and stirring alternately in a positive and negative direction to obtain a nutritive seed; the process not only breaks the dormancy state of the fragrant fruit tree seeds, but also provides certain nutrient components for the seeds, improves the life vitality of the fragrant fruit tree seeds and provides required nutrients for the subsequent germination of the fragrant fruit tree seeds; scattering the nutritional seeds on fine sand, regularly turning and spraying water to obtain the dewy fragrant fruit tree seeds; and (3) inoculating the chamomile tree seeds on a germination culture medium to obtain seedlings. The combined action of the steps effectively shortens the germination period of the seeds of the fragrant fruit tree and improves the germination rate of the seeds of the fragrant fruit tree.
(2) By the method for culturing the seeds of the myrtle trees, the germination period can be as short as 7 days, and the germination rate is as high as 98.2%.
(3) The method for culturing the seeds of the myrtle trees, provided by the invention, is simple in culture mode and suitable for popularization and application.
Detailed Description
In order to make the technical solutions of the present invention better understood and enable those skilled in the art to practice the present invention, the following embodiments are further described, but the present invention is not limited to the following embodiments. The following test methods and detection methods, unless otherwise specified, are conventional methods; the reagents and starting materials are all commercially available unless otherwise specified.
Example 1
A method for culturing seeds of a fragrant fruit tree comprises the following steps:
s1: seed pretreatment: collecting fragrant fruit tree seeds with full seeds and high maturity, ultrasonically cleaning, airing, and soaking in a seed soaking agent to obtain pretreated seeds;
the ultrasonic cleaning frequency is 0.5kHz, and the ultrasonic cleaning time is 10min.
The seed soaking agent contains 0.05 percent of amobam solution and 0.1 percent of sodium chloride solution. The volume ratio of the ambam solution to the sodium chloride solution is 1.
Before the seeds of the fragrant fruit tree are soaked by the seed soaking agent, the seeds of the fragrant fruit tree are disinfected firstly, and the treatment steps are as follows: soaking the ultrasonic-cleaned seeds of the myrtle trees in a 0.01 mass percent tacrine solution for 20S, cleaning with sterile water, drying, and performing radiation treatment on the seeds for 10min by blue light with the wavelength of 435 nm.
S2: endowing nutrition: performing positive and negative bidirectional alternating magnetic heating stirring on the pretreated seeds in the S1 in a nutrient solution to obtain nutritive seeds;
the nutrient solution is prepared from the following raw materials in parts by weight: 5 parts of livestock and poultry manure, 10 parts of passion fruit residues, 10 parts of nitrogen-phosphorus-potassium compound fertilizer, 3 parts of coal ash and 500 parts of water.
The magnetic heating stirring is carried out by adopting a JB-1 type timing bidirectional magnetic stirrer of Heizhou gold jar Liangyou instruments Co., ltd, the temperature is 20 ℃, the stirring speed is 800r/min, and the stirring time is 1h.
S3: white culture: spreading fine sand on a plurality of layers of porous gauze, scattering the nutritional seeds S2 on the fine sand, turning over regularly and spraying water to obtain the lubai Xiangguo tree seeds;
the porous gauze and the fine sand are both soaked with a germination accelerating solution, and the germination accelerating solution is a cyanamide aqueous solution with the mass fraction of 0.01%.
The temperature of the white exposure culture is 26 ℃, the humidity is 75%, the illumination intensity is 1800Lx, and the illumination time is 5 h/day.
S4: germination culture: and (4) inoculating the dewing myrtle tree seeds in the S3 on a germination culture medium to obtain seedlings.
The formula of the germination medium comprises 8g/L agar powder, 15g/L white granulated sugar, 2g/L passion fruit juice, 5g/L corn flour, 3g/L bran and 1g/L active carbon.
Example 2
A method for culturing seeds of a fragrant fruit tree comprises the following steps:
s1: seed pretreatment: collecting fragrant fruit tree seeds with full seeds and high maturity, ultrasonically cleaning, airing, and soaking in a seed soaking agent to obtain pretreated seeds;
the ultrasonic cleaning frequency is 1kHz, and the ultrasonic cleaning time is 30min.
The seed soaking agent contains 0.25 mass percent of ambam solution and 0.4 mass percent of sodium chloride solution. The volume ratio of the ambam solution to the sodium chloride solution is 1.
Before the seeds of the fragrant fruit tree are soaked by the seed soaking agent, the seeds of the fragrant fruit tree are disinfected firstly, and the treatment steps are as follows: soaking the ultrasonic-cleaned seeds of the myrtle trees in a 0.03 mass percent tacrine solution for 40S, cleaning with sterile water, drying, and performing radiation treatment for 20min by using blue light with the wavelength of 450 nm.
S2: endowing nutrition: performing positive and negative bidirectional alternating magnetic heating stirring on the pretreated seeds in the S1 in a nutrient solution to obtain nutritive seeds;
the nutrient solution is prepared from the following raw materials in parts by weight: 8 parts of livestock and poultry manure, 20 parts of passion fruit residues, 15 parts of nitrogen-phosphorus-potassium compound fertilizer, 5 parts of fly ash and 700 parts of water.
The temperature of the magnetic heating stirring is 30 ℃, the stirring speed is 1500r/min, and the stirring time is 3h.
S3: white culture: spreading fine sand on a plurality of layers of porous gauze, scattering the nutritive seeds of S2 on the fine sand, turning over regularly and spraying water to obtain the fragrant fruit tree seeds exposed to white;
the porous gauze and the fine sand are both soaked with a germination accelerating solution, and the germination accelerating solution is a cyanamide aqueous solution with the mass fraction of 0.05%.
The temperature of the white exposure culture is 30 ℃, the humidity is 85%, the illumination intensity is 2000Lx, and the illumination time is 7 days.
S4: and (3) germination culture: and (4) inoculating the dewy fragrant fruit tree seeds in the S3 on a germination culture medium to obtain seedlings.
The formula of the germination medium comprises agar powder with the concentration of 10g/L, white granulated sugar with the concentration of 20g/L, passion fruit juice with the concentration of 4g/L, corn flour with the concentration of 8g/L, bran with the concentration of 6g/L and active carbon with the concentration of 1.5 g/L.
Example 3
A method for culturing seeds of a fragrant fruit tree comprises the following steps:
s1: seed pretreatment: collecting fragrant fruit tree seeds with full seeds and high maturity, ultrasonically cleaning, airing, and soaking in a seed soaking agent to obtain pretreated seeds;
the ultrasonic cleaning frequency is 0.8kHz, and the ultrasonic cleaning time is 20min.
The seed soaking agent contains 0.15 mass percent of ambam solution and 0.25 mass percent of sodium chloride solution. The volume ratio of the ambam solution to the sodium chloride solution is 1.
Before the seeds of the fragrant fruit tree are soaked by the seed soaking agent, the seeds of the fragrant fruit tree are disinfected firstly, and the treatment steps are as follows: soaking the ultrasonic-cleaned Xiangguo tree seeds in a 0.02 mass percent tacrine solution for 30S, cleaning with sterile water, drying, and performing radiation treatment on the Xiangguo tree seeds for 15min by using blue light with the wavelength of 440 nm.
S2: endowing nutrition: performing positive and negative bidirectional alternating magnetic heating stirring on the pretreated seeds in the S1 in a nutrient solution to obtain nutritive seeds;
the nutrient solution is prepared from the following raw materials in parts by weight: 6.5 parts of livestock and poultry manure, 15 parts of passion fruit residues, 12 parts of nitrogen-phosphorus-potassium compound fertilizer, 4 parts of fly ash and 600 parts of water.
The temperature of the magnetic heating stirring is 25 ℃, the stirring speed is 1150r/min, and the stirring time is 2h.
S3: white culture: spreading fine sand on a plurality of layers of porous gauze, scattering the nutritional seeds S2 on the fine sand, turning over regularly and spraying water to obtain the lubai Xiangguo tree seeds;
the porous gauze and the fine sand are both soaked with a germination accelerating solution, and the germination accelerating solution is a cyanamide aqueous solution with the mass fraction of 0.03%.
The temperature of the white exposure culture is 28 ℃, the humidity is 80%, the illumination intensity is 1900Lx, and the illumination time is 6 h/day.
S4: germination culture: and (4) inoculating the dewing myrtle tree seeds in the S3 on a germination culture medium to obtain seedlings.
The formula of the germination culture medium comprises 9g/L agar powder, 17.5g/L white granulated sugar, 3g/L passion fruit juice, 6.5g/L corn flour, 4.5g/L bran and 1.2g/L active carbon.
Example 4
A method for culturing seeds of a fragrant fruit tree comprises the following steps:
s1: seed pretreatment: collecting fragrant fruit tree seeds with full seeds and high maturity, ultrasonically cleaning, airing, and soaking in a seed soaking agent to obtain pretreated seeds;
the frequency of ultrasonic cleaning is 0.7kHz, and the ultrasonic cleaning time is 15min.
The seed soaking agent contains 0.1 mass percent of ambam solution and 0.3 mass percent of sodium chloride solution. The volume ratio of the ambam solution to the sodium chloride solution is 1.
Before the seeds of the fragrant fruit tree are soaked by the seed soaking agent, the seeds of the fragrant fruit tree are disinfected firstly, and the treatment steps are as follows: soaking the ultrasonic-cleaned fructus amomi tree seeds in a 0.015 percent tacrine solution for 25S, cleaning with sterile water, airing, and carrying out radiation treatment on the seeds for 12min by using blue light with the wavelength of 445 nm.
S2: endowing nutrition: performing positive and negative bidirectional alternating magnetic heating stirring on the pretreated seeds in the S1 in a nutrient solution to obtain nutritive seeds;
the nutrient solution is prepared from the following raw materials in parts by weight: 6 parts of livestock manure, 12 parts of passion fruit residues, 13 parts of nitrogen-phosphorus-potassium compound fertilizer, 3.5 parts of coal ash and 550 parts of water.
The temperature of the magnetic heating stirring is 22 ℃, the stirring speed is 1000r/min, and the stirring time is 1.5h.
S3: white culture: spreading fine sand on a plurality of layers of porous gauze, scattering the nutritional seeds S2 on the fine sand, turning over regularly and spraying water to obtain the lubai Xiangguo tree seeds;
the porous gauze and the fine sand are both soaked with a germination accelerating solution, and the germination accelerating solution is a cyanamide aqueous solution with the mass fraction of 0.02%.
The temperature of the white exposure culture is 27 ℃, the humidity is 77%, the illumination intensity is 1850Lx, and the illumination time is 5.5 h/day.
S4: and (3) germination culture: and (4) inoculating the dewing myrtle tree seeds in the S3 on a germination culture medium to obtain seedlings.
The formula of the germination medium comprises 8.5g/L agar powder, 16g/L white granulated sugar, 2.5g/L passion fruit juice, 6g/L corn flour, 4g/L bran and 1.3g/L active carbon.
Example 5
A method for culturing seeds of a fragrant fruit tree comprises the following steps:
s1: seed pretreatment: collecting fragrant fruit tree seeds with full seeds and high maturity, ultrasonically cleaning, airing, and soaking in a seed soaking agent to obtain pretreated seeds;
the ultrasonic cleaning frequency is 0.6kHz, and the ultrasonic cleaning time is 25min.
The seed soaking agent contains 0.2 mass percent of ambam solution and 0.2 mass percent of sodium chloride solution. The volume ratio of the ambam solution to the sodium chloride solution is 1.
Before the seeds of the fragrant fruit tree are soaked by the seed soaking agent, the seeds of the fragrant fruit tree are disinfected firstly, and the treatment steps are as follows: soaking the ultrasonic-cleaned seeds of the myrtle trees in 0.025 percent of procymidone solution for 35S, cleaning with sterile water, drying, and performing radiation treatment for 18min by using blue light with the wavelength of 437 nm.
S2: endowing nutrition: performing positive and negative bidirectional alternating magnetic heating stirring on the pretreated seeds in the S1 in a nutrient solution to obtain nutritive seeds;
the nutrient solution is prepared from the following raw materials in parts by weight: 7 parts of livestock and poultry manure, 18 parts of passion fruit residues, 14 parts of nitrogen-phosphorus-potassium compound fertilizer, 4.5 parts of coal ash and 650 parts of water.
The temperature of the magnetic heating stirring is 28 ℃, the stirring speed is 1300r/min, and the stirring time is 2.5h.
S3: white culture: spreading fine sand on a plurality of layers of porous gauze, scattering the nutritional seeds S2 on the fine sand, turning over regularly and spraying water to obtain the lubai Xiangguo tree seeds;
the porous gauze and the fine sand are both soaked with a germination accelerating solution, and the germination accelerating solution is a cyanamide aqueous solution with the mass fraction of 0.04%.
The temperature of the white exposure culture is 29 ℃, the humidity is 82%, the illumination intensity is 1950Lx, and the illumination time is 6.5 h/day.
S4: and (3) germination culture: and (4) inoculating the dewy fragrant fruit tree seeds in the S3 on a germination culture medium to obtain seedlings.
The formula of the germination medium comprises 9.5g/L agar powder, 18.5g/L white granulated sugar, 3.5g/L passion fruit juice, 7g/L corn flour, 5g/L bran and 1.4g/L active carbon.
In order to further illustrate the effect of the present invention, the present invention is further provided with a comparative example, as follows:
comparative example 1
A method for culturing seeds of a fragrant fruit tree comprises the following steps:
s1: seed pretreatment: collecting fragrant fruit tree seeds with full seeds and high maturity, ultrasonically cleaning, airing, and soaking in a seed soaking agent to obtain pretreated seeds;
the ultrasonic cleaning frequency is 0.8kHz, and the ultrasonic cleaning time is 20min.
The seed soaking agent contains 0.15 mass percent of ambam solution and 0.25 mass percent of sodium chloride solution. The volume ratio of the ambam solution to the sodium chloride solution is 1.
Before the seeds of the fragrant fruit tree are soaked by the seed soaking agent, the seeds of the fragrant fruit tree are disinfected firstly, and the treatment steps are as follows: soaking the ultrasonic-cleaned fructus Amomi rotundus seeds in 0.02 wt% Tanking solution for 30S, cleaning with sterile water, air drying, and treating with blue light with wavelength of 440nm for 15min.
S2: white culture: spreading fine sand on a plurality of layers of porous gauze, scattering the nutritional seeds S2 on the fine sand, turning over regularly and spraying water to obtain the lubai Xiangguo tree seeds;
the porous gauze and the fine sand are both soaked with a germination accelerating solution, and the germination accelerating solution is a cyanamide aqueous solution with the mass fraction of 0.03%.
The temperature of the white exposure culture is 28 ℃, the humidity is 80%, the illumination intensity is 1900Lx, and the illumination time is 6 h/day.
S3: and (3) germination culture: and (3) inoculating the dewy fragrant fruit tree seeds in the S2 on a germination culture medium to obtain seedlings.
The formula of the germination medium comprises 9g/L agar powder, 17.5g/L white granulated sugar, 3g/L passion fruit juice, 6.5g/L corn flour, 4.5g/L bran and 1.2g/L active carbon.
Comparative example 2
A method for culturing seeds of a fragrant fruit tree comprises the following steps:
s1: seed pretreatment: collecting fragrant fruit tree seeds with full seeds and high maturity, ultrasonically cleaning, airing, and soaking in a seed soaking agent to obtain pretreated seeds;
the ultrasonic cleaning frequency is 0.8kHz, and the ultrasonic cleaning time is 20min.
The seed soaking agent contains 0.15 mass percent of ambam solution and 0.25 mass percent of sodium chloride solution.
Before the seeds of the fragrant fruit tree are soaked by the seed soaking agent, the seeds of the fragrant fruit tree are disinfected firstly, and the treatment steps are as follows: soaking the ultrasonic-cleaned fructus Amomi rotundus seeds in 0.02 wt% Tanking solution for 30S, cleaning with sterile water, air drying, and treating with blue light with wavelength of 440nm for 15min.
The volume ratio of the ambam solution to the sodium chloride solution is 1.
S2: endowing nutrition: performing positive and negative bidirectional alternating magnetic heating stirring on the pretreated seeds in the S1 in a nutrient solution to obtain nutritive seeds;
the nutrient solution is prepared from the following raw materials in parts by weight: 6.5 parts of livestock and poultry manure, 15 parts of passion fruit residues, 12 parts of nitrogen-phosphorus-potassium compound fertilizer, 4 parts of fly ash and 600 parts of water.
The temperature of the magnetic heating stirring is 25 ℃, the stirring speed is 1150r/min, and the stirring time is 2h.
S3: germination culture: and (3) inoculating the nutritional seeds in the S2 on a germination culture medium to obtain seedlings.
The formula of the germination medium comprises 9g/L agar powder, 17.5g/L white granulated sugar, 3g/L passion fruit juice, 6.5g/L corn flour, 4.5g/L bran and 1.2g/L active carbon.
Comparative example 3
A method for culturing seeds of a fragrant fruit tree comprises the following steps:
s1: seed pretreatment: collecting fragrant fruit tree seeds with full seeds and high maturity, ultrasonically cleaning, airing, and soaking in a seed soaking agent to obtain pretreated seeds;
the ultrasonic cleaning frequency is 0.8kHz, and the ultrasonic cleaning time is 20min.
The seed soaking agent contains 0.15 mass percent of ambam solution and 0.25 mass percent of sodium chloride solution.
Before the seeds of the fragrant fruit tree are soaked by the seed soaking agent, the seeds of the fragrant fruit tree are disinfected firstly, and the treatment steps are as follows: soaking the ultrasonic-cleaned Xiangguo tree seeds in a 0.02 mass percent tacrine solution for 30S, cleaning with sterile water, drying, and performing radiation treatment on the Xiangguo tree seeds for 15min by using blue light with the wavelength of 440 nm.
The volume ratio of the ambam solution to the sodium chloride solution is 1.
S2: endowing nutrition: performing positive and negative bidirectional alternating magnetic heating stirring on the pretreated seeds in the S1 in a nutrient solution to obtain nutritive seeds;
the nutrient solution is prepared from the following raw materials in parts by weight: 6.5 parts of livestock and poultry manure, 15 parts of passion fruit residues, 12 parts of nitrogen-phosphorus-potassium compound fertilizer, 4 parts of fly ash and 600 parts of water.
The temperature of the magnetic heating stirring is 25 ℃, the stirring speed is 1150r/min, and the stirring time is 2h.
S3: white culture: spreading fine sand on a plurality of layers of porous gauze, scattering the nutritional seeds S2 on the fine sand, turning over regularly and spraying water to obtain the lubai Xiangguo tree seeds;
the porous gauze and the fine sand are both soaked with a germination accelerating solution, and the germination accelerating solution is a cyanamide aqueous solution with the mass fraction of 0.03%.
The temperature of the white exposure culture is 28 ℃, the humidity is 80%, the illumination intensity is 1900Lx, and the illumination time is 6 h/day.
S4: germination culture: and (4) inoculating the lubai Xiangguo tree seeds in the S3 into soil to obtain seedlings.
The germination conditions of the myrtle seeds and the nutrient components of the myrtle seeds provided in the above examples 1 to 5 and comparative examples 1 to 3 were respectively detected, and the results were as follows:
(1) And (3) germination period: the germination cycles of the seeds of the myrtle trees cultivated in examples 1 to 5 were 9 days, 7 days, 9 days, 8 days and 9 days, respectively, while the germination cycles of the seeds of the myrtle trees cultivated in comparative examples 1 to 3 were 20 days, 16 days and 17 days, respectively.
(2) The germination rate is as follows: the germination rates of the seeds of the myrtle trees cultivated in examples 1 to 5 were 97.5%, 98.2%, 96.2%, 95.4% and 93.1%, respectively, while the germination rates of the seeds of the myrtle trees cultivated in comparative examples 1 to 3 were 71.4%, 82.6% and 83.1%, respectively.
(3) And (3) detecting nutrient components: the seeds of the kouma tree cultivated in comparative example 1 were not subjected to nutrient treatment, and the vitamin a, vitamin B1 and B2, vitamin C, phosphorus, calcium, iron and moisture contents in the seeds were 4 μ g, 0.01mg, 0.03mg, 2.8mg, 90mg, 17mg, 2.0mg and 40%, respectively, whereas the nutritional ingredients of the seeds of the kouma tree cultivated in examples 1 to 5 were similar, and the seeds of the kouma tree were 5.1 μ g, 0.03mg, 0.06mg, 4.5mg, 103mg, 20mg, 3.8mg and 55%, respectively, in the example 3 alone.
In conclusion, the method for culturing the fragrant fruit tree seeds effectively improves the nutrient components stored in the fragrant fruit tree seeds, thereby further shortening the germination period of the fragrant fruit tree seeds and improving the germination rate of the fragrant fruit tree seeds, and compared with comparative examples 1-3, the method for culturing the fragrant fruit tree seeds in examples 1-5 has the germination period as short as one week and the germination rate is as high as 98.2%; compared with comparative example 2, the content of vitamin a, vitamins B1 and B2, vitamin C, phosphorus, calcium, iron and moisture in the myrtle seeds cultured in examples 1 to 5 were 5.1 μ g, 0.03mg, 0.06mg, 4.5mg, 103mg, 20mg, 3.8mg and 55%, respectively; the invention has obvious effects of improving the nutrient content in the seeds of the fragrant fruit trees, shortening the germination period and improving the germination rate.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (5)
1. The method for culturing the seeds of the myrtle trees is characterized by comprising the following steps of:
s1: seed pretreatment: collecting mature fragrant fruit tree seeds, ultrasonically cleaning, airing, and soaking in a seed soaking agent to obtain pretreated seeds; the seed soaking agent contains 0.05-0.25 mass percent of ambam solution and 0.1-0.4 mass percent of sodium chloride solution; the volume ratio of the ambam solution to the sodium chloride solution is 1;
s2: endowing nutrition: performing positive and negative bidirectional alternating magnetic heating stirring on the pretreated seeds in the S1 in a nutrient solution to obtain nutritive seeds; the nutrient solution is prepared from the following raw materials in parts by weight: 5-8 parts of livestock and poultry manure, 10-20 parts of passion fruit residues, 10-15 parts of nitrogen-phosphorus-potassium compound fertilizer, 3-5 parts of fly ash and 500-700 parts of water;
s3: white culture: spreading fine sand on a plurality of layers of porous gauze, scattering the nutritional seeds S2 on the fine sand, turning over regularly and spraying water to obtain the lubai Xiangguo tree seeds; the porous gauze and the fine sand are both soaked with a germination accelerating solution, and the germination accelerating solution is a cyanamide aqueous solution with the mass fraction of 0.01-0.05%;
s4: and (3) germination culture: inoculating the acerola tree seeds in the S3 on a germination culture medium to obtain seedlings; the formula of the germination culture medium comprises 8-10 g/L agar powder, 15-20 g/L white granulated sugar, 2-4 g/L passion fruit juice, 5-8 g/L corn flour and 3-6 g/L bran, and 1-1.5 g/L active carbon.
2. The method for culturing the seeds of the myrtle tree according to claim 1, wherein in S2, the magnetic heating and stirring temperature is 20-30 ℃, the stirring speed is 800-1500 r/min, and the stirring time is 1-3 h.
3. The method for culturing the seeds of the myrtle tree according to claim 1, wherein in S3, the temperature of the dew white culture is 26-30 ℃, the humidity is 75-85%, the illumination intensity is 1800-2000 Lx, and the illumination time is 5-7 h/day.
4. The method for culturing the seeds of the myrtle tree according to claim 1, wherein in S1, the ultrasonic cleaning frequency is 0.5 kHz-1 kHz, and the ultrasonic cleaning time is 10-30 min.
5. The method for culturing the seeds of the myrtle tree according to claim 1, wherein before the seeds of the myrtle tree are soaked by the seed soaking agent, the seeds of the myrtle tree are disinfected, and the treatment steps are as follows: soaking the fragrant fruit tree seeds subjected to ultrasonic cleaning in a procymidone solution with the mass fraction of 0.01-0.03% for 20-40 s, cleaning with sterile water, drying in the air, and performing radiation treatment for 10-20 min by using blue light with the wavelength of 435-450 nm.
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