CN114796968A - Preparation method of algae active high-efficiency crude oil degradation agent - Google Patents

Preparation method of algae active high-efficiency crude oil degradation agent Download PDF

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CN114796968A
CN114796968A CN202210393688.9A CN202210393688A CN114796968A CN 114796968 A CN114796968 A CN 114796968A CN 202210393688 A CN202210393688 A CN 202210393688A CN 114796968 A CN114796968 A CN 114796968A
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algae
mixed
crude oil
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oil degradation
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贺朝文
张乐
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Alger Life Sciences Jiangsu Co ltd
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    • A—HUMAN NECESSITIES
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    • A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
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    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
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Abstract

The invention discloses a preparation method of an algae active high-efficiency crude oil degradation agent, relates to the technical field of crude oil degradation agents, and specifically relates to 45% of chlorella liquid, 45% of fossa-shaped basket algae mixed liquid and 10% of mixed bone powder. The preparation method of the algae active high-efficiency crude oil degradation agent can provide enough nutrients for soil after the degradation agent enters the soil, so that microorganisms in the honeycomb basket algae mixed solution can live and absorb the nutrients, the increase of the number of the microorganisms can loosen the soil and increase the contact area with the outside, the natural evaporation of the crude oil in the soil can be increased, and meanwhile, the microorganisms need grease as food for survival, so that the content of the crude oil in the soil can be reduced, and the microorganisms in the chlorella can release a large amount of oxygen in the proliferation process so as to supply the microorganisms in the honeycomb basket algae mixed solution to live, thereby avoiding the situation that the oxygen required by plant growth is deprived while the oil concentration of the soil is degraded, meanwhile, the mixed bone meal is also mixed into the soil, and being beneficial to improving the air permeability of the soil and providing nutrients for the plant growth.

Description

Preparation method of algae active high-efficiency crude oil degradation agent
Technical Field
The invention relates to the technical field of crude oil degradation agents, in particular to a preparation method of an algae active high-efficiency crude oil degradation agent.
Background
Soil oil concentration generally refers to the concentration of oil composition in the soil, can definitely know the condition of this soil through detecting soil oil concentration, can know whether this soil accords with vegetation based on the detection conditions, have very important directive significance to agricultural production, and need carry out crude oil degradation to the soil that oil concentration exceeds standard, and soil oil concentration exceeds standard and marks this soil and has appeared crude oil pollution, is not suitable for planting the plant, so need use the crude oil degradation agent.
The existing crude oil degradation agent mainly adopts pollution-free biodegradation, namely, oil pollution fields are repaired by the activity of microbial enzymes, the environment, inorganic nutrition, fertilizers, the types of microorganisms and the like, but the microbial enzymes consume a large amount of oxygen during metabolism and multiplication, and easily capture the amount of oxygen required by plant growth.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a preparation method of an algae active high-efficiency crude oil degradation agent, which solves the problems that the existing crude oil degradation agent in the background art is mainly subjected to pollution-free biodegradation, namely, an oil pollution field is repaired through the activity of microbial enzymes, the environment, inorganic nutrition, fertilizers, the types of microorganisms and the like, but the microbial enzymes consume a large amount of oxygen during metabolic reproduction and easily capture the amount of oxygen required by plant growth.
In order to achieve the purpose, the invention is realized by the following technical scheme: the preparation method of the algae active high-efficiency crude oil degradation agent comprises the following raw materials in percentage by mass:
45% of chlorella liquid, 45% of fossa-shaped basket algae mixed liquid and 10% of mixed bone powder.
Optionally, the preparation method of the mixed solution of the honeycomb basket algae comprises the following steps:
placing the honeycomb basket algae in a glass container, mashing with a glass rod, washing with normal saline, and repeatedly washing for three times to obtain wet algae;
weighing wet algae 1g, adding KH with concentration of 1/15M 2 PO 4 -Na 2 HPO 4 25ml buffer (pH7.2), shaking to obtain mixture of Phoenix punctatus, and adding 25ml KH 1/15M 1g wet algae 2 PO 4 -Na 2 HPO 4 Buffer (pH7.2), both increased at the same ratio.
Optionally, the preparation method of the mixed bone meal comprises the following steps:
soaking the bones of the livestock in clear water, heating the water until bubbles are generated until floating froth floats on the water surface, taking out the bones of the livestock after removing the froth, drying the bones of the livestock in a drying device, and then breaking and grinding the bones of the livestock to obtain bone powder;
and (3) taking the bone meal into a mixing device, adding lactic acid bacteria, saccharomycetes and photosynthetic bacteria into the mixing device, mixing and stirring, and uniformly stirring to obtain the mixed bone meal.
Optionally, the temperature of the livestock bone is controlled to be 40-50 ℃ during drying, and the bone powder is ground and then screened by using screens of 50 meshes, 100 meshes and 200 meshes in sequence.
Optionally, the mass ratio of the bone meal to the lactic acid bacteria, the yeast and the photosynthetic bacteria is 1:0.1:0.1:0.1, and the temperature of the bone meal to the lactic acid bacteria, the yeast and the photosynthetic bacteria is 30-35 ℃ when the bone meal is mixed.
Optionally, the preparation method of the algae-activated high-efficiency crude oil degradation agent comprises the following operation steps:
placing the chlorella liquid in a stirring device, adding the fossa-shaped basket algae mixed liquid, stirring, adding the mixed bone powder after uniformly stirring, and continuously stirring until the three are fully and uniformly mixed to obtain the algae active high-efficiency crude oil degradation agent.
Optionally, the mass ratio of the chlorella liquid to the sedum nidulans mixed liquid is 1:1, and the mass ratio of the mixed liquid of the chlorella liquid and the sedum nidulans mixed liquid to the mixed bone powder is 9: 1.
Optionally, the temperature of the mixed chlorella solution and the mixed chlorella honeycomb basket is 35-40 ℃, and the temperature of the mixed chlorella solution and the mixed chlorella basket powder is 45 ℃.
Optionally, the concentration of the chlorella solution is 200 ten thousand per mL, and the concentration of the fossa-shaped basket algae mixed solution is 200 ten thousand per mL.
The invention provides a preparation method of an algae active high-efficiency crude oil degradation agent, which has the following beneficial effects:
the preparation method of the algae active high-efficiency crude oil degradation agent comprises the steps that the chlorella liquid contains balanced nutrition of various mineral substances, vitamins, cellulose, folic acid, nucleic acid, calcium, magnesium, iron, zinc, various mineral substances, vitamins and the like, the degradation agent can provide enough nutrients for soil after entering the soil, the microorganisms in the chlorella mixed liquid can survive and absorb the nutrients, the increase of the number of the microorganisms can loosen the soil and increase the contact area with the outside, the natural evaporation of the crude oil in the soil can be increased, meanwhile, the microorganisms need grease as food for survival, the content of the crude oil in the soil can be reduced, the microorganisms in the chlorella can release a large amount of oxygen in proliferation so as to supply the microorganisms in the chlorella mixed liquid for survival, meanwhile, the oxygen can be provided for plant growth, the oxygen required for plant growth can be avoided being deprived while the oil concentration of the soil is degraded, meanwhile, the mixed bone powder is also mixed into the soil, which is beneficial to improving the air permeability of the soil and providing nutrients for the growth of plants.
Drawings
FIG. 1 is a schematic diagram of an experiment of the algae-derived active highly efficient crude oil degradation agent of the present invention.
Detailed Description
The invention provides a technical scheme that: a preparation method of an algae active high-efficiency crude oil degradation agent comprises the following raw materials in percentage by mass:
45% of chlorella liquid, 45% of fossa-shaped basket algae mixed liquid and 10% of mixed bone powder.
The preparation method of the honeycomb basket algae mixed solution comprises the following steps:
placing the honeycomb basket algae in a glass container, mashing with a glass rod, washing with normal saline, and repeatedly washing for three times to obtain wet algae;
weighing wet algae 1g, adding KH with concentration of 1/15M 2 PO 4 -Na 2 HPO 4 25ml buffer (pH7.2), shaking to obtain mixture of Phoenix punctatus, and adding 25ml KH 1/15M 1g wet algae 2 PO 4 -Na 2 HPO 4 Buffer (pH7.2), both increased at the same ratio.
The preparation method of the mixed bone meal comprises the following steps:
soaking the bones of the livestock in clear water, heating the water until bubbles are generated until floating froth floats on the water surface, taking out the bones of the livestock after removing the froth, drying the bones of the livestock in a drying device, and then breaking and grinding the bones of the livestock to obtain bone powder;
and (3) taking the bone meal into a mixing device, adding lactic acid bacteria, saccharomycetes and photosynthetic bacteria into the mixing device, mixing and stirring, and uniformly stirring to obtain the mixed bone meal.
The temperature of the livestock bone is controlled to be 40-50 ℃ when the livestock bone is dried, and the bone powder is ground and then screened by using screens of 50 meshes, 100 meshes and 200 meshes in sequence.
The mass ratio of the bone meal to the lactic acid bacteria, the microzyme and the photosynthetic bacteria is 1:0.1:0.1, and the temperature of the bone meal to the lactic acid bacteria, the microzyme and the photosynthetic bacteria is 30-35 ℃ when the bone meal is mixed.
The preparation method of the algae active high-efficiency crude oil degradation agent comprises the following operation steps:
placing the chlorella liquid in a stirring device, adding the fossa-shaped basket algae mixed liquid, stirring, adding the mixed bone powder after uniformly stirring, and continuously stirring until the three are fully and uniformly mixed to obtain the algae active high-efficiency crude oil degradation agent.
The mass ratio of the chlorella solution to the fossa-shaped basket algae mixed solution is 1:1, and the mass ratio of the mixed solution of the chlorella solution and the fossa-shaped basket algae mixed solution to the mixed bone meal is 9: 1.
The temperature of the mixed solution of the chlorella solution and the honeycomb basket algae is 35-40 ℃, and the temperature of the mixed solution of the chlorella solution and the honeycomb basket algae mixed solution is 45 ℃ when the mixed solution and the mixed bone meal are mixed.
The concentration of the chlorella solution is 200 ten thousand per mL, and the concentration of the fossa-shaped basket algae mixed solution is 200 ten thousand per mL;
the preparation method of the algae active high-efficiency crude oil degradation agent comprises the steps that the chlorella liquid contains balanced nutrition of various mineral substances, vitamins, cellulose, folic acid, nucleic acid, calcium, magnesium, iron, zinc, various mineral substances, vitamins and the like, the degradation agent can provide enough nutrients for soil after entering the soil, the microorganisms in the chlorella mixed liquid can survive and absorb the nutrients, the increase of the number of the microorganisms can loosen the soil and increase the contact area with the outside, the natural evaporation of the crude oil in the soil can be increased, meanwhile, the microorganisms need grease as food for survival, the content of the crude oil in the soil can be reduced, the microorganisms in the chlorella can release a large amount of oxygen in proliferation so as to supply the microorganisms in the chlorella mixed liquid for survival, meanwhile, the oxygen can be provided for plant growth, the oxygen required for plant growth can be avoided being deprived while the oil concentration of the soil is degraded, meanwhile, the mixed bone powder is also mixed into the soil, which is beneficial to improving the air permeability of the soil and providing nutrients for the growth of plants.
Example (b):
the alga active high-efficiency crude oil degradation agent obtained by the step configuration is taken as an experimental sample, under the condition that the temperature is 25 ℃, the alga active high-efficiency crude oil degradation agent is dripped into n-tetradecane, the initial concentration (A) of the n-tetradecane is 77.0mg/L, after the alga active high-efficiency crude oil degradation agent is processed in 2500Lux light intensity for 3 hours, the residual concentration (C) of the alga active high-efficiency crude oil degradation agent is reduced to 55.6mg/L, and the residual concentration (B) of a control group which is not added with the alga active high-efficiency crude oil degradation agent is still maintained at 74.8mg/L, the details are shown in figure 1, which shows that the alga active high-efficiency crude oil degradation agent has the hydrocarbon removing effect.
In conclusion, the preparation method of the algae activity high-efficiency crude oil degradation agent comprises the steps of putting the honeycomb basket algae into a glass container, mashing the basket algae with a glass rod, washing with normal saline water, and repeatedly washing for three times for later use, so that wet algae are obtained; weighing wet algae 1g, adding KH with concentration of 1/15M 2 PO 4 -Na 2 HPO 4 25ml buffer (pH7.2), shaking to obtain mixture of Phoenix punctatus, and adding 25ml KH 1/15M 1g wet algae 2 PO 4 -Na 2 HPO 4 Buffer (pH7.2), both increase at the same ratio;
then, taking the livestock bones, soaking in clear water, heating the water until bubbles are generated, removing the floating foam, taking out the livestock bones, drying in a drying device, and then breaking and grinding the livestock bones to obtain bone powder; taking the bone meal into a mixing device, adding lactic acid bacteria, saccharomycetes and photosynthetic bacteria into the mixing device, mixing and stirring, and obtaining mixed bone meal after uniformly stirring;
finally, placing the chlorella liquid in a stirring device, adding the pit shaped basket algae mixed liquid, stirring, adding the mixed bone powder after uniformly stirring, and continuously stirring until the three are fully and uniformly mixed to obtain the algae active high-efficiency crude oil degradation agent;
the preparation method of the algae active high-efficiency crude oil degradation agent comprises the steps that the chlorella liquid contains balanced nutrition of various mineral substances, vitamins, cellulose, folic acid, nucleic acid, calcium, magnesium, iron, zinc, various mineral substances, vitamins and the like, the degradation agent can provide enough nutrients for soil after entering the soil, the microorganisms in the chlorella mixed liquid can survive and absorb the nutrients, the increase of the number of the microorganisms can loosen the soil and increase the contact area with the outside, the natural evaporation of the crude oil in the soil can be increased, meanwhile, the microorganisms need grease as food for survival, the content of the crude oil in the soil can be reduced, the microorganisms in the chlorella can release a large amount of oxygen in proliferation so as to supply the microorganisms in the chlorella mixed liquid for survival, meanwhile, the oxygen can be provided for plant growth, the oxygen required for plant growth can be avoided being deprived while the oil concentration of the soil is degraded, meanwhile, the mixed bone powder is also mixed into the soil, which is beneficial to improving the air permeability of the soil and providing nutrients for the growth of plants.

Claims (9)

1. A preparation method of an algae active high-efficiency crude oil degradation agent is characterized by comprising the following steps: the algae active high-efficiency crude oil degradation agent comprises the following raw materials in percentage by mass:
45% of chlorella liquid, 45% of fossa-shaped basket algae mixed liquid and 10% of mixed bone powder.
2. The method for preparing the algae-activated high-efficiency crude oil degradation agent according to claim 1, wherein the steps of: the preparation method of the fossa matting algae mixed solution comprises the following steps:
placing the honeycomb basket algae in a glass container, mashing with a glass rod, washing with normal saline, and repeatedly washing for three times to obtain wet algae;
weighing wet algae 1g, adding KH with concentration of 1/15M 2 PO 4 -Na 2 HPO 4 25ml buffer (pH7.2), shaking to obtain mixture of Phoenix punctatus, and adding 25ml KH 1/15M 1g wet algae 2 PO 4 -Na 2 HPO 4 Buffer (pH7.2), both increased at the same ratio.
3. The method for preparing the algae-activated high-efficiency crude oil degradation agent according to claim 1, wherein the steps of: the preparation method of the mixed bone meal comprises the following steps:
soaking the bones of the livestock in clear water, heating the water until bubbles are generated until floating froth floats on the water surface, taking out the bones of the livestock after removing the froth, drying the bones of the livestock in a drying device, and then breaking and grinding the bones of the livestock to obtain bone powder;
and (3) taking the bone meal into a mixing device, adding lactic acid bacteria, saccharomycetes and photosynthetic bacteria into the mixing device, mixing and stirring, and uniformly stirring to obtain the mixed bone meal.
4. The method for preparing the algae-activated high-efficiency crude oil degradation agent according to claim 3, wherein the steps of: the temperature of the livestock bone is controlled to be 40-50 ℃ during drying, and after the bone powder is ground, screens of 50 meshes, 100 meshes and 200 meshes are sequentially used for screening.
5. The method for preparing the algae-activated high-efficiency crude oil degradation agent according to claim 3, wherein the steps of: the mass ratio of the bone meal to the lactic acid bacteria, the yeasts and the photosynthetic bacteria is 1:0.1:0.1:0.1, and the temperature of the bone meal to the lactic acid bacteria, the yeasts and the photosynthetic bacteria is 30-35 ℃ when the bone meal is mixed.
6. The method for preparing the algae-activating high-efficiency crude oil degradation agent according to any one of claims 1 to 5, wherein the steps of: the preparation method of the algae active high-efficiency crude oil degradation agent comprises the following operation steps:
placing the chlorella liquid in a stirring device, adding the fossa-shaped basket algae mixed liquid, stirring, adding the mixed bone powder after uniformly stirring, and continuously stirring until the three are fully and uniformly mixed to obtain the algae active high-efficiency crude oil degradation agent.
7. The method for preparing the algae-activating high-efficiency crude oil degradation agent according to claim 6, wherein the steps of: the mass ratio of the chlorella liquid to the fossa sarmentosum mixed liquid is 1:1, and the mass ratio of the mixed liquid of the chlorella liquid and the fossa sarmentosum mixed liquid to the mixed bone powder is 9: 1.
8. The method for preparing the algae-activating high-efficiency crude oil degradation agent according to claim 6, wherein the steps of: the temperature of the chlorella liquid and the fossa-shaped basket algae mixed liquid is 35-40 ℃, and the temperature of the mixed liquid of the chlorella liquid and the fossa-shaped basket algae mixed liquid is 45 ℃ when the mixed liquid and the mixed bone powder are mixed.
9. The method for preparing the algae-activating high-efficiency crude oil degradation agent according to any one of claims 1 to 8, wherein the steps of: the concentration of the chlorella solution is 200 ten thousand per mL, and the concentration of the fossa-shaped basket algae mixed solution is 200 ten thousand per mL.
CN202210393688.9A 2022-04-15 2022-04-15 Preparation method of algae active high-efficiency crude oil degradation agent Pending CN114796968A (en)

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2296112C1 (en) * 2005-09-26 2007-03-27 Государственное научное учреждение Всероссийский научно-исследовательский институт орошаемого земледелия РАСХН Method for production of fermented agriculture composition
CN101235096A (en) * 2008-01-25 2008-08-06 中国地质大学（武汉） Method for extracting polysaccharide from phormidium tenue
US20150104855A1 (en) * 2013-10-12 2015-04-16 Nankai University Enhanced combination method for phytoremediation of petroleum contaminated soil by Impatiens balsamina L. associated with the indigenous microorganisms
CN105779359A (en) * 2016-04-21 2016-07-20 厦门人天景农业科技有限公司 Microbial preparation for improving heavy metals in soil
CN109173700A (en) * 2018-10-09 2019-01-11 盐城市锦瑞石油机械有限公司 A kind of device for high concentration petroleum emission of degrading
CN110330983A (en) * 2019-08-21 2019-10-15 阿尔格生命科学(江苏)有限公司 A kind of active high-effect heavy metal degradation agent of algae
CN110437841A (en) * 2019-08-21 2019-11-12 阿尔格生命科学(江苏)有限公司 A kind of active high-effect oil degradation agent of algae
CN111285711A (en) * 2020-03-27 2020-06-16 内蒙古锡牧生命科学有限公司 Plant nutrient composition and preparation method and application thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2296112C1 (en) * 2005-09-26 2007-03-27 Государственное научное учреждение Всероссийский научно-исследовательский институт орошаемого земледелия РАСХН Method for production of fermented agriculture composition
CN101235096A (en) * 2008-01-25 2008-08-06 中国地质大学（武汉） Method for extracting polysaccharide from phormidium tenue
US20150104855A1 (en) * 2013-10-12 2015-04-16 Nankai University Enhanced combination method for phytoremediation of petroleum contaminated soil by Impatiens balsamina L. associated with the indigenous microorganisms
CN105779359A (en) * 2016-04-21 2016-07-20 厦门人天景农业科技有限公司 Microbial preparation for improving heavy metals in soil
CN109173700A (en) * 2018-10-09 2019-01-11 盐城市锦瑞石油机械有限公司 A kind of device for high concentration petroleum emission of degrading
CN110330983A (en) * 2019-08-21 2019-10-15 阿尔格生命科学(江苏)有限公司 A kind of active high-effect heavy metal degradation agent of algae
CN110437841A (en) * 2019-08-21 2019-11-12 阿尔格生命科学(江苏)有限公司 A kind of active high-effect oil degradation agent of algae
CN111285711A (en) * 2020-03-27 2020-06-16 内蒙古锡牧生命科学有限公司 Plant nutrient composition and preparation method and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
沈韫芬等: "《微型生物监测新技术》", 31 July 1990, 中国建筑工业出版社 *

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Application publication date: 20220729