CN105779359A - Microbial preparation for improving heavy metals in soil - Google Patents
Microbial preparation for improving heavy metals in soil Download PDFInfo
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- CN105779359A CN105779359A CN201610252293.1A CN201610252293A CN105779359A CN 105779359 A CN105779359 A CN 105779359A CN 201610252293 A CN201610252293 A CN 201610252293A CN 105779359 A CN105779359 A CN 105779359A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Abstract
The invention discloses a microbial preparation for improving heavy metals in soil. The microbial preparation comprises photosynthetic bacteria, actinomycetes, lactic acid bacteria, saccharomycetes, bacillus subtilis, bacillus thuringiensis, bacillus megatherium, nitrogen-fixing bacteria, citric acid germs, bacillus licheniformis and a matrix for providing nutrition for the flora. The microbial preparation for improving the heavy metals in soil can degrade the heavy metals in the soil safely and efficiently in an environment-friendly manner, and can meet the demand for social and economic development.
Description
Technical field
The invention belongs to soil remediation technical field, relate to a kind of soil conditioner, be specifically related to a kind of microorganism agent for improveing heavy metal-polluted soil.
Background technology
Soil is the important natural resources of human survival and development, but, whole nation soil environment situation totally allows of no optimist at present, and some areas soil pollution is heavier, and arable soil environmental quality causes anxiety, industry and mining discarded ground soil environment outstanding problem.The human activity such as industry and mining, agricultural and Environmental Background Values of Soils height are to cause soil pollution or the main cause exceeded standard.The whole nation total exceeding standard rate of soil is 16.1%, wherein slightly, slightly, moderate and serious pollution point position ratio respectively 11.2%, 2.3%, 1.5% and 1.1%.Pollution type is based on inorganic type, and organic type takes second place, and compound pollution proportion is less, and an inorganic pollution figure place that exceeds standard accounts for the 82.8% of all super punctuation bits.
Exceed standard situation from pollutant, cadmium, hydrargyrum, arsenic, copper, lead, chromium, zinc, 8 kinds of inorganic pollution object point position exceeding standard rate of nickel respectively 7.0%, 1.6%, 2.7%, 2.1%, 1.5%, 1.1%, 0.9%, 4.8%.
From polluting distribution situation, soils in south china pollutes and overweights the north；The subregion soil contamination problem such as Yangtze River Delta, the Delta of the Pearl River, Northeastern rust belt are comparatively prominent, southwest, variable charge soil heavy metals exceeding standard scope bigger；Cadmium, hydrargyrum, arsenic, plumbous 4 kinds of level of inorganic contaminants distribution present the situation gradually risen from northwest to the southeast, from northeast to southwestward.
The improvement of heavy metal-polluted soil is very urgent.At present, the reparation of heavy metal pollution mainly has two kinds of approach: 1, change the existence of heavy metal, reduces its activity so that it is passivation, departs from food chain, reduce its toxicity.2, special plant is utilized to absorb the heavy metal in soil, then this plant is removed or by engineering heavy metal become soluble state, free state, then through drip washing, then collect the heavy metal in leacheate, thus reaching reclaim heavy metal and reduce the dual purpose of heavy metal in soil.The method adopted both at home and abroad generally can be divided into engineering measure, agricultural measures, ameliorative measure and biological control measure.
Place heavy-metal contaminated soil reparation is the heavy metal utilizing the transfer of physics, chemical and biological method, absorbing, degrade and convert in soil so that it is concentration is reduced to acceptable level, meets the requirement of corresponding land use pattern.According to technology category, place heavy metal pollution of soil reparation can be divided into peripheral doses, chemical redemption, biological restoration, combine d bioremediation and Agro-ecology reparation etc..
But the Transport And Transformation of heavy metal pollution of soil thing is extremely complex, it is extremely difficult to administer, and therefore, researches and develops the reagent and method that can quickly, effectively improve heavy metal-polluted soil and technology is urgent.
Summary of the invention
In view of this, present invention aims to the present situation that current heavy metal pollution of soil is serious, it is provided that a kind of environmental friendliness, can fast and effeciently heavy metal-polluted soil be carried out the microorganism agent administered Yu improve.
Concrete, the microorganism agent for improveing heavy metal-polluted soil of the present invention, in mass fraction, including following components:
1 part of concentration is 1.1～1.5*108The photosynthetic bacteria of individual/g, 1 part of concentration are 3.3～3.5*108The actinomycetes of individual/g, 1 part of concentration are 1.1～1.5*108The lactic acid bacteria of individual/g, 1 part of concentration are 2～3*108The yeast of individual/g, 2 parts of concentration are 6～8*108The bacillus subtilis of individual/g, 1 part of concentration are 1～2*108The bacillus thuringiensis of individual/g, 1 part of concentration are 5～6*108The bacillus megaterium of individual/g, 1 part of concentration are 2～3*108The nitrogen-fixing bacteria of individual/g, 1 part of concentration are 2～2.5*108The citric acid bacterium of individual/g, 2 parts of concentration are 1.1～1.5*108The substrate of the Bacillus licheniformis of individual/g and 5～10 times of above-mentioned flora mass parts provided nutrition.
Preferably, in mass fraction, described substrate comprises following component:
Testa Tritici 25～30 parts, 25～30 parts of Testa oryzae, bean cake 15～20 parts, milk powder 6～10 parts, Cortex Cinnamomi 5～8 parts, shell-fish bone meal 5～8 parts, magnesium oxide 2～3 parts, milt 6～8 parts, 4～6 parts of molasses；Wherein, described Testa Tritici, Testa oryzae can single also can simultaneously arbitrarily ratio mixing as substrate.
Further, in mass fraction, described substrate comprises following component:
Testa Tritici 25 parts, 25 parts of Testa oryzae, bean cake 20 parts, milk powder 8 parts, Cortex Cinnamomi 5 parts, shell-fish bone meal 5 parts, magnesium oxide 2 parts, milt 6 parts, 4 parts of molasses.
The microorganism agent for improveing heavy metal-polluted soil of the present invention, it is possible to obtain by extracting the profitable strain in cattle manure.First the profitable strain that can react is chosen with heavy metal-polluted soil, then these profitable strains are implanted in plant, resupply and herd cattle fooder, chew digestion by the four of cattle stomaches, excrete finally by feces, then in the feces of cattle, these profitable strains are extracted again.In the process, these floras keep activity, and therefore, the profitable strain extracted has high activity, and in heavy metal-polluted soil governance process, effect is notable.
The substrate that the microorganism agent for improveing heavy metal-polluted soil of the present invention adopts is mainly used in providing nutrition for beneficial microbe colony therein, beneficial microbe colony can be attached in substrate under 10～70 DEG C of states and keep sleep state, multiple beneficial bacterium can be coexisted with peacefully and be preserved, do not produce antagonism, during use, only need dilute, so that it may making beneficial microbe colony revive, solidarity reacts with heavy metal in soil.In soil environment, microorganism species can utilize effective nutrition and the energy, is carried out directly or indirectly by various metabolic activities, all has effect for various soil environments, including highly acid, faintly acid, strong basicity, alkalescence etc..
The present invention for improveing the microorganism agent of heavy metal-polluted soil, can safe and environment-friendly, the heavy metal in degraded soil efficiently, meet socio-economic development demand.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be described in detail below, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under the premise not making creative work, broadly fall into the scope of protection of the invention.
Embodiment 1
nullSoil improvement microorganism agent is mixed with Testa oryzae or Testa Tritici and is uniformly sprayed on soil by the ratio of 1:5 in mass ratio，Wherein，Soil improvement microorganism agent is mainly composed of microorganism species，Specifically include photosynthetic bacteria 1kg (concentration is 1.1～1.5 hundred million/g)、Actinomycetes 1kg (concentration is 3.3～3.5 hundred million/g)、Lactic acid bacteria 1kg (concentration is 1.1～1.5 hundred million/g)、Yeast 1kg (concentration is 2～300,000,000/g)、Bacillus subtilis 2kg (concentration is 6～800,000,000/g)、Bacillus thuringiensis 1kg (concentration is 1～200,000,000/g)、Bacillus megaterium 1kg (concentration is 5～600,000,000/g)、Nitrogen-fixing bacteria 1kg (concentration is 2～300,000,000/g)、Citric acid bacillus 1kg (concentration is 2～2.5 hundred million/g)、Bacillus licheniformis 2kg (concentration is 1.1～1.5 hundred million/g).Testa oryzae used herein or Testa Tritici also can substitute with other materials, rich in the frumentum of nutrient substance including bean dregs, rice flour, distiller grains etc., because of Testa oryzae or Testa Tritici is inexpensive and can reach the nutritional need of microorganism, therefore, it is suggested that use Testa oryzae or Testa Tritici.The consumption that makes of soil improvement microorganism agent is 2kg/ mu, then plough, soil and microorganism agent is made to be fully contacted, then irrigate, irrigate the degree of depth and keep 15cm～20cm, relative moisture of the soil keeps about 80%, now, microorganism agent is fully activated, microorganism species utilizes the effective nutrition in soil, carry out various metabolic activity, react with the heavy metal in soil directly or indirectly, through the effect of 5～7 days, heavy metal content in soil can be reduced, if effect is not reaching to standard, standard herein is country's Soil standard (see table 1), repeatable operation 1～2 time, can produce a desired effect, namely the soil environment condition of national regulation is met.
Table 1 standard of soil environment quality value GB15618-1995 unit: mg/kg
Wherein, primary standard is protection zone natural environment, the limits value maintaining the soil quality of natural background.Secondary standard, for ensureing agricultural production, safeguards the soil limits value of health.Grade III Standard is the soil phosphorus forms ensureing agricultural and forestry production and plant normal growth.
Through 5 days time, testing soil heavy metal component and content, its result is in Table 2.
Table 2 heavy metal content in soil contrast unit: mg/kg
| Soil | Copper | Zinc | Ferrum | Manganese | Arsenic | Hydrargyrum | Plumbous | Cadmium | Chromium |
| Untreated | 16.98 | 0.76 | 13.7 | 5.6 | 5.46 | 0.04 | 18.6 | 0.069 | 54.12 |
| Process 5 days | 0.77 | 0.56 | 12.2 | 4.8 | 4.85 | 0.032 | 17.21 | 0.063 | 53.3 |
| Degradation rate (%) | 95.5 | 26.3 | 10.9 | 14.3 | 11.2 | 20 | 7.5 | 8.7 | 1.5 |
Embodiment 2
nullSoil improvement microorganism agent is mixed with distiller grains and is uniformly sprayed on sorghum field by the ratio of 1:10 in mass ratio，Wherein，Soil improvement microorganism agent is mainly composed of microorganism species，Specifically include photosynthetic bacteria 1kg (concentration is 1.1～1.5 hundred million/g)、Actinomycetes 1kg (concentration is 3.3～3.5 hundred million/g)、Lactic acid bacteria 1kg (concentration is 1.1～1.5 hundred million/g)、Yeast 1kg (concentration is 2～300,000,000/g)、Bacillus subtilis 2kg (concentration is 6～800,000,000/g)、Bacillus thuringiensis 1kg (concentration is 1～200,000,000/g)、Bacillus megaterium 1kg (concentration is 5～600,000,000/g)、Nitrogen-fixing bacteria 1kg (concentration is 2～300,000,000/g)、Citric acid bacillus 1kg (concentration is 2～2.5 hundred million/g)、Bacillus licheniformis 2kg (concentration is 1.1～1.5 hundred million/g).The consumption that makes of soil improvement microorganism agent is 3kg/ mu, then plough, make soil and microorganism agent be fully contacted, then irrigate, irrigate the degree of depth and keep 15cm～20cm, relative moisture of the soil keeps about 80%, now, microorganism agent is fully activated, and microorganism species utilizes the effective nutrition in soil, carry out various metabolic activity, react with the heavy metal in soil directly or indirectly.
Through 7 days testing soil heavy metal component and content, its result is in Table 3.
Table 3 heavy metal content in soil contrast unit: mg/kg
| Soil | Copper | Zinc | Ferrum | Manganese | Arsenic | Hydrargyrum | Plumbous | Cadmium | Chromium |
| Untreated | 19.88 | 0.86 | 13.7 | 5.6 | 5.86 | 0.068 | 20.86 | 0.094 | 62.02 |
| Process 7 days | 0.89 | 0.55 | 14.3 | 4.8 | 4.88 | 0.046 | 18.57 | 0.077 | 60.41 |
| Degradation rate (%) | 95.5 | 36 | -4.3 | 14.3 | 16.7 | 32.3 | 11 | 18 | 2.6 |
As can be seen here, the copper in soil, zinc, manganese, arsenic, hydrargyrum, lead, cadmium, chromium are respectively provided with Degradation by the microorganism agent for improveing heavy metal-polluted soil of the present invention, and the cycle of process is short, and effect is notable.
Wherein, the content of ferrum is up, and main reason is that microbial bacteria passes through the reactions such as absorption, metabolism, is separated by heavy metal element or reduce its toxicity, being converted into beneficial element (such as ferrum element) simultaneously.
In those skilled in the art, it is clear that the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when without departing substantially from the spirit of the present invention or basic feature, it is possible to realize the present invention in other specific forms.Therefore, no matter from which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the invention rather than described above limits, it is intended that all changes in the implication of the equivalency dropping on claim and scope included in the present invention.
In addition, it is to be understood that, although this specification is been described by according to embodiment, but not each embodiment only comprises an independent technical scheme, this narrating mode of description is only for clarity sake, description should be made as a whole by those skilled in the art, and the technical scheme in each embodiment through appropriately combined, can also form other embodiments that it will be appreciated by those skilled in the art that.
Claims (3)
1. the microorganism agent being used for improveing heavy metal-polluted soil, it is characterised in that in mass fraction, including following components:
1 part of concentration is 1.1 ~ 1.5*108The photosynthetic bacteria of individual/g, 1 part of concentration are 3.3 ~ 3.5*108The actinomycetes of individual/g, 1 part of concentration are 1.1 ~ 1.5*108The lactic acid bacteria of individual/g, 1 part of concentration are 2 ~ 3*108The yeast of individual/g, 2 parts of concentration are 6 ~ 8*108The bacillus subtilis of individual/g, 1 part of concentration are 1 ~ 2*108The bacillus thuringiensis of individual/g, 1 part of concentration are 5 ~ 6*108The bacillus megaterium of individual/g, 1 part of concentration are 2 ~ 3*108The nitrogen-fixing bacteria of individual/g, 1 part of concentration are 2 ~ 2.5*108The citric acid bacterium of individual/g, 2 parts of concentration are 1.1 ~ 1.5*108The substrate of the Bacillus licheniformis of individual/g and 5 ~ 10 times of above-mentioned flora mass parts provided nutrition.
2. microorganism agent according to claim 1, it is characterized in that, in mass fraction, described substrate comprises following component: Testa Tritici 25 ~ 30 parts, 25 ~ 30 parts of Testa oryzae, bean cake 15 ~ 20 parts, milk powder 6 ~ 10 parts, Cortex Cinnamomi 5 ~ 8 parts, shell-fish bone meal 5 ~ 8 parts, magnesium oxide 2 ~ 3 parts, milt 6 ~ 8 parts, 4 ~ 6 parts of molasses；Wherein, described Testa Tritici, Testa oryzae can single also can simultaneously arbitrarily ratio mixing as substrate.
3. microorganism agent according to claim 2, it is characterized in that, in mass fraction, described substrate comprises following component: Testa Tritici 25 parts, 25 parts of Testa oryzae, bean cake 20 parts, milk powder 8 parts, Cortex Cinnamomi 5 parts, shell-fish bone meal 5 parts, magnesium oxide 2 parts, milt 6 parts, 4 parts of molasses.
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106238456A (en) * | 2016-09-21 | 2016-12-21 | 成都创慧科达科技有限公司 | A kind of microbial composite bacteria substrate of rehabilitating soil and application thereof |
| CN106607454A (en) * | 2016-12-20 | 2017-05-03 | 平顶山学院 | Remediation method for chrome-polluted soil in coal mine collapse region |
| CN106964647A (en) * | 2017-06-06 | 2017-07-21 | 临沂大学 | A kind of method of utilization composite bacteria agent remediating lead-contaminated soil |
| CN106975657A (en) * | 2017-06-05 | 2017-07-25 | 华定志 | Utilize the technique of bioanalysis rehabilitating soil arsenic pollution |
| CN107321785A (en) * | 2017-07-27 | 2017-11-07 | 西北农林科技大学 | A kind of method that use microorganism growth stimulator administers soil pollution |
| CN107446861A (en) * | 2017-09-06 | 2017-12-08 | 北京双龙阿姆斯科技有限公司 | Microbial bacterial agent of degraded heavy-metal residual and preparation method thereof |
| CN107497838A (en) * | 2017-01-20 | 2017-12-22 | 明志科技大学 | The method that removing heavy metals are removed from soil |
| CN108384731A (en) * | 2018-02-05 | 2018-08-10 | 华中农业大学 | A kind of manganese oxidizing bacteria and its screening technique and application |
| CN109201725A (en) * | 2018-08-24 | 2019-01-15 | 盐城英贝吉环保科技有限公司 | A kind of contaminated soil remediation device |
| CN109954751A (en) * | 2017-12-25 | 2019-07-02 | 梅滨 | A kind of soil green ecological restorative procedure |
| CN111471608A (en) * | 2019-11-28 | 2020-07-31 | 中南林业科技大学 | Strain capable of adsorbing manganese and promoting growth of plants and application thereof |
| CN113695383A (en) * | 2020-05-23 | 2021-11-26 | 中国环境科学研究院 | Plant restoration device and restoration method for heavy metal pollution of soil |
| CN113695382A (en) * | 2020-05-23 | 2021-11-26 | 中国环境科学研究院 | Microorganism soil layer dispersion mixing inoculation method |
| CN114105409A (en) * | 2021-11-19 | 2022-03-01 | 苏州中晟环境修复有限公司 | Method for treating antimony in underground water polluted by printing and dyeing wastewater |
| CN114796968A (en) * | 2022-04-15 | 2022-07-29 | 阿尔格生命科学(江苏)有限公司 | Preparation method of algae active high-efficiency crude oil degradation agent |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106238456A (en) * | 2016-09-21 | 2016-12-21 | 成都创慧科达科技有限公司 | A kind of microbial composite bacteria substrate of rehabilitating soil and application thereof |
| CN106607454A (en) * | 2016-12-20 | 2017-05-03 | 平顶山学院 | Remediation method for chrome-polluted soil in coal mine collapse region |
| CN107497838A (en) * | 2017-01-20 | 2017-12-22 | 明志科技大学 | The method that removing heavy metals are removed from soil |
| CN106975657A (en) * | 2017-06-05 | 2017-07-25 | 华定志 | Utilize the technique of bioanalysis rehabilitating soil arsenic pollution |
| CN106975657B (en) * | 2017-06-05 | 2020-06-23 | 江西源春环保科技有限公司 | Process for remedying arsenic pollution of soil by biological method |
| CN106964647A (en) * | 2017-06-06 | 2017-07-21 | 临沂大学 | A kind of method of utilization composite bacteria agent remediating lead-contaminated soil |
| CN107321785A (en) * | 2017-07-27 | 2017-11-07 | 西北农林科技大学 | A kind of method that use microorganism growth stimulator administers soil pollution |
| CN107446861B (en) * | 2017-09-06 | 2024-02-27 | 北京双龙阿姆斯科技有限公司 | Microbial agent for degrading heavy metal residues and preparation method thereof |
| CN107446861A (en) * | 2017-09-06 | 2017-12-08 | 北京双龙阿姆斯科技有限公司 | Microbial bacterial agent of degraded heavy-metal residual and preparation method thereof |
| CN109954751A (en) * | 2017-12-25 | 2019-07-02 | 梅滨 | A kind of soil green ecological restorative procedure |
| CN108384731A (en) * | 2018-02-05 | 2018-08-10 | 华中农业大学 | A kind of manganese oxidizing bacteria and its screening technique and application |
| CN109201725A (en) * | 2018-08-24 | 2019-01-15 | 盐城英贝吉环保科技有限公司 | A kind of contaminated soil remediation device |
| CN111471608B (en) * | 2019-11-28 | 2022-09-13 | 中南林业科技大学 | Strain capable of adsorbing manganese and promoting growth of plants and application thereof |
| CN111471608A (en) * | 2019-11-28 | 2020-07-31 | 中南林业科技大学 | Strain capable of adsorbing manganese and promoting growth of plants and application thereof |
| CN113695383A (en) * | 2020-05-23 | 2021-11-26 | 中国环境科学研究院 | Plant restoration device and restoration method for heavy metal pollution of soil |
| CN113695382A (en) * | 2020-05-23 | 2021-11-26 | 中国环境科学研究院 | Microorganism soil layer dispersion mixing inoculation method |
| CN113695382B (en) * | 2020-05-23 | 2023-08-15 | 中国环境科学研究院 | Microbial soil layer dispersion mixing inoculation method |
| CN114105409A (en) * | 2021-11-19 | 2022-03-01 | 苏州中晟环境修复有限公司 | Method for treating antimony in underground water polluted by printing and dyeing wastewater |
| CN114796968A (en) * | 2022-04-15 | 2022-07-29 | 阿尔格生命科学(江苏)有限公司 | Preparation method of algae active high-efficiency crude oil degradation agent |
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Application publication date: 20160720 |