CN114793776A

CN114793776A - Method for greening river coastal ground cover

Info

Publication number: CN114793776A
Application number: CN202210352810.8A
Authority: CN
Inventors: 曹华
Original assignee: Huzhou Zhongheng Garden Construction Co ltd
Current assignee: Huzhou Zhongheng Garden Construction Co ltd
Priority date: 2022-03-31
Filing date: 2022-03-31
Publication date: 2022-07-29

Abstract

The invention discloses a method for greening land cover along the bank of a river, which comprises the steps of artificially inoculating arbuscular mycorrhizal fungi to calamus seedlings, obtaining arbuscular mycorrhizal fungi-calamus symbiont seeds after the seedlings are mature, and sowing the arbuscular mycorrhizal fungi-calamus symbiont seeds to the bank of the river, wherein the root system of the arbuscular mycorrhizal fungi-calamus symbiont has strong ground holding capacity and strong flood resistance and scouring capacity, so that the flood control effect can be improved.

Description

Method for greening river coastal ground cover

Technical Field

The invention relates to the field of ecological greening, in particular to a method for greening river riparian land.

Background

Flood is a natural phenomenon that water quantity in rivers and lakes is rapidly increased or water level is rapidly increased due to natural factors such as rainstorm, rapid ice and snow melting, storm surge and the like, and is a natural disaster. The loss of flood to human society is very serious, and the flood comes temporarily, and the vegetation of river coastal is destroyed, and the rubble and soft earth of river bank gets into the river along with the flood, and the riverbed is lifted, accelerates the ageing of river.

At present, grass and trees planted in the river bank greening mainly comprise dryopteris sinica, groundsel, alocasia esculenta, semipennywort, Chinese knotweed and the like, and when the grass and trees are planted singly, the root systems of the plants are often not developed enough, the ground grabbing effect is not good, the flood resistance and the flushing capability are poor, and the flood control effect is poor; in the mixed seed, the flood control effect is improved to a certain extent, but the subsequent management becomes troublesome.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a method for greening the riparian land of a river, which has the advantage of good flood control effect.

In order to achieve the purpose, the invention provides the following technical scheme:

a method for greening the coastal land of a river comprises the following steps:

s1: culturing arbuscular mycorrhizal fungi-calamus symbiont at least comprising the following steps:

s11: sterilizing calamus seeds;

s12: placing the calamus seeds into a culture medium for culturing to obtain calamus aseptic seedlings;

s13: artificially inoculating strain on the calamus aseptic seedling;

s14: cleaning up the culture medium of the root of the inoculated calamus seedling, transferring the culture medium into a culture dish filled with a sterilization culture medium for culture until the harvest period, placing the culture dish in an incubator with sufficient illumination for culture, keeping the temperature at 20-25 ℃, and culturing under the conditions that:

in the first month: spraying one half of Hoagland's nutrient solution every ten days, and keeping the culture medium slightly dry;

and in the second month: spraying Hoagland's nutrient solution with halved phosphorus element once every ten days, and keeping the culture medium slightly dry;

after the second month: spraying Hoagland's total nutrient solution every ten days, and keeping the culture medium moist until the harvest period;

s2: and (3) detecting the inoculation condition of the fungi after culturing for a period of time, selecting and discarding the calamus plants which are not infected by the fungi, and culturing the rest calamus plants to a harvesting period to obtain arbuscular mycorrhizal fungi-calamus symbiont seeds.

S3: sowing the cultured arbuscular mycorrhizal fungi-calamus symbiont seeds to the coasts of rivers;

s4: subsequent weeding and water management.

By adopting the technical scheme, the arbuscular mycorrhizal fungi-calamus symbiont seeds are prepared and planted in a sowing mode, the survival rate is higher than that of the planting in a separate mode, by adopting the method, the arbuscular mycorrhizal fungi-calamus symbiont does not need to be re-cultivated every year, only the seeds need to be collected, and the seeds are sowed regularly, so that the difficulty in planting the arbuscular mycorrhizal fungi-calamus symbiont is greatly reduced. The symbiont has a more developed and robust root system and stronger soil-holding power, so that the flood scouring resistance is improved, meanwhile, the absorption capacity of the calamus plants infected by the arbuscular mycorrhizal fungi on nitrogen and phosphorus in water is greatly improved, and the eutrophication of the water body can be improved to a certain degree. The calamus seeds are sterilized, so that no other fungi can appear when the arbuscular mycorrhizal fungi-calamus symbiont is cultured, and the process that the arbuscular mycorrhizal fungi infect the calamus plants is influenced. The artificial inoculation can improve the infection rate of arbuscular mycorrhizal fungi on calamus plants and obtain more symbionts with successful infection. The root of the calamus seedling is very young, tender and fragile in the first month, the requirement for growth of the calamus seedling can be guaranteed by spraying one half of Hoagland's nutrient solution once, the root burning phenomenon cannot occur due to overhigh concentration of the nutrient solution, the culture medium is kept slightly dry, the phenomenon of root rot can be reduced, and the growth of the root of the calamus seedling can be promoted. In the second month, the root of the calamus plant is infected by arbuscular mycorrhizal fungi to form a symbiont, and the phosphorus absorption can be increased through the produced extra-root hypha, so that the content of phosphorus in the Hoagland's nutrient solution is required to be halved, and the culture medium is kept slightly dry to prevent the root from rotting and promote the root to grow. After the second month, the arbuscular mycorrhizal fungi-calamus symbiont grows to the adult stage, all aspects are stable, and at the moment, Hoagland's full nutrient solution is selected, so that sufficient nutrition can be provided for the arbuscular mycorrhizal fungi-calamus symbiont.

Further, the preparation method of the culture medium in S12 comprises the following steps: 30g of sterile agar was melted in 1L of sterile water and then cooled to solidify.

By adopting the technical scheme, the culture medium made of agar and water is transparent, so that the cleanness of the culture medium can be ensured, and the growth condition of the calamus seeds can be observed.

Further, the artificial inoculation at least comprises the following steps:

s131: vertically cutting the embryonic axis of the calamus aseptic seedling, wherein the incision depth is 2-3 mm;

s132: and (3) filling hypha of the arbuscular mycorrhizal fungi into the cut, performing dark culture for 7-10 days at the constant temperature of 23-27 ℃, and performing light culture for 7-10 days after dark culture.

Through adopting above-mentioned technical scheme, the hypocotyl of seedling can develop into the junction of connecting stem and root in the future, the infection rate that hypha infects can be guaranteed to inoculation hypha here, and can not hinder the root of seedling, lead to the seedling death, in the field, the temperature that optimum calamus seedling grows is between 23 ~ 27 ℃, 7 ~ 10 days after the manual work connects the fungus need carry out the dark culture, this is because the incision and the hypha of initial stage calamus plant do not fuse, it can dry fast to accept illumination messenger's incision, can't be symbiotic with the hypha, hypha needs to grow in the environment of darkness simultaneously, illumination can destroy the activity of hypha, hypha and calamus seedling fuse the completion after 7 ~ 10 days, need carry out illumination this moment and let calamus seedling carry out photosynthesis, continue to grow up to harvest time.

Further, the arbuscular mycorrhizal fungus is any one of saccharomyces mosissima/saccharomyces youngicus/saccharomyces intraradiculatus.

By adopting the technical scheme, the Moses sacculus mildew/young sacculus glochidionis/sacculus mycorrhizae in roots has strong infection capacity and strong hyphae, and is more suitable for artificial inoculation. Sacculus mould mosaici/young sacculus mould of cover/sacculus mould in root can promote the growth of calamus root, improve the ability that calamus resisted adverse environment, and the calamus plant that is infected by sacculus mould mosaici/young sacculus mould of cover/sacculus mould in root promotes the absorptive capacity of aquatic nitrogen phosphorus greatly simultaneously, can improve the eutrophication of water to a certain extent.

Further, the preparation steps of the culture medium are as follows:

s121: sieving river sand with a 16-mesh sieve to obtain fine sand;

s122: mixing fine sand and loam according to the mass ratio of 3: 1 to obtain a culture medium;

s123: and sterilizing the culture substrate to obtain the sterilized culture substrate.

By adopting the technical scheme, the river sand has good drainage and air permeability and can improve the air permeability of the loam by being mixed with the loam, and the river sand has no fertility and has little influence on the control of nutrient substances in subsequent culture. The loam has the advantages of clay and sandy soil, and the culture medium prepared by mixing the loam with river sand has good air permeability, water retention and heat preservation performance, and can provide proper soil conditions for cultivating the arbuscular mycorrhizal fungi-calamus symbiont.

Further, the culture medium is sterilized by placing the culture medium in a reaction kettle, and maintaining at 103.4KPa and 121 deg.C for 20 min.

By adopting the technical scheme, the mixed bacteria in the culture medium are killed at high temperature, so that the influence of the mixed bacteria on the root of the calamus infected by the arbuscular mycorrhizal fungi is reduced.

Further, the step of sowing the arbuscular mycorrhizal fungi-calamus symbiont seeds to the coasts of rivers comprises the following steps:

s31: taking the area in front of the arbor along the river shore as a sowing area of arbuscular mycorrhizal fungi-calamus symbiont seeds;

s32: deeply ploughing soil in the planting area, spraying disinfectant for disinfection, and adding coarse sand to improve air permeability;

s33: sowing the arbuscular mycorrhizal fungi-calamus symbiont seeds into the sterilized soil, controlling the distance between the seeds to be 30-35 cm, covering the soil, and compacting.

By adopting the technical scheme, the area in front of the trees along the river bank is basically a blank area and is rich in weeds, the sowing area of the arbuscular mycorrhizal fungi-calamus symbiont seeds is selected to be close to the river, and the soil with high water content is more suitable for growth of calamus. The calamus needs to grow in penetrating soil, the air permeability of soil is too low to cause the rotten of calamus leaf, adds the coarse sand and can improve the air permeability of soil, and the more suitable calamus grows. The distance between every two plants is 30-35 cm, so that sufficient growth space can be provided for the calamus, and the condition of poor plant development caused by over crowding is avoided.

Furthermore, each gram of hypha contains 5-7 arbuscular mycorrhizal fungi spores.

By adopting the technical scheme, the phenomenon that the plant root system is dead due to unsuccessful infection of the fungus can occur when the spores are too few, and the growth of the plant can be influenced when the arbuscular mycorrhizal fungus is propagated in large quantity when the spores are too many. When each gram of hypha contains 5-7 arbuscular mycorrhizal fungal spores, not only can the plant root system be successfully infected by the fungi be guaranteed, but also the effect of promoting the plant growth by the fungi can be achieved.

Further, the dosage of each raw material is as follows:

sterilizing a culture medium: 160-560 g

The calamus seeds: 5 to 19 particles

Hypha: 0.4 to 2g

By adopting the technical scheme, a large amount of sterilization culture mediums can provide sufficient rooting space for the calamus seeds, the seeding density is kept between 160 and 560g of sterilization culture mediums for 5 to 19 calamus seeds, and when the density exceeds the range, insufficient moisture is caused around the seeds, so that the emergence rate is low.

Further, the use amount of each raw material is preferably:

sterilizing a culture medium: 360g

The calamus seeds: 12 granules

Hypha: 1g

By adopting the technical scheme, 360g of the sterilization culture medium, 12 calamus seeds and 1g of hypha are the optimal selection, and the germination of the calamus seeds and the infection of arbuscular mycorrhizal fungi on the plant root system can achieve better effects.

Further, the formula of the Hoagland's nutrient solution is as follows:

one-half Hoagland's nutrient solution: 472.5mg/L of calcium nitrate, 303.5mg/L of potassium nitrate, 57.5mg/L of ammonium phosphate, 246.5mg/L of magnesium sulfate, 1.75ml/L of iron salt solution and a mass ratio of urea 30: sodium dihydrogen phosphate 120: the trace element liquid of potassium sulfate 87 is 2.5 ml/L.

Hoagland's nutrient solution with halved phosphorus element: 945mg/L calcium nitrate, 607mg/L potassium nitrate, 115mg/L ammonium phosphate, 493mg/L magnesium sulfate, 2.5ml/L iron salt solution and 5ml/L trace element solution with the mass ratio of 30 parts of urea, 60 parts of sodium dihydrogen phosphate and 87 parts of potassium sulfate.

Hoagland's complete nutrient solution: 945mg/L calcium nitrate, 607mg/L potassium nitrate, 115mg/L ammonium phosphate, 493mg/L magnesium sulfate, 2.5ml/L iron salt solution and 5ml/L trace element solution with the mass ratio of 30 parts of urea, 120 parts of sodium dihydrogen phosphate and 87 parts of potassium sulfate.

By adopting the technical scheme, the Hoagland's nutrient solution can be subjected to targeted nutrient supplement aiming at plants in different periods by changing the formula of the Hoagland's nutrient solution, so that the plants can grow healthily and rapidly.

Further, weeding and water and fertilizer management comprises:

weeding management: weeding once in three days in the previous week after transplanting, and weeding once in every week after one week;

water management: watering is carried out once a day in the previous week after transplanting, and watering is carried out once every three days after one week of transplanting.

By adopting the technical scheme, weeding can provide a better environment for the growth of plants, the phenomenon that other plants compete for nutrients with the arbuscular mycorrhizal fungi-calamus symbiont is avoided, a large amount of water is needed for the growth of calamus, a large amount of water is needed for promoting rooting in the initial stage of transplanting, the transplanted arbuscular mycorrhizal fungi-calamus symbiont is watered regularly to be capable of quickly rooting, and the survival rate of transplanting is improved.

Detailed Description

The present invention will be described in further detail with reference to examples.

A method for greening river riparian land at least comprises the following steps:

s1: culturing arbuscular mycorrhizal fungi-calamus symbiont, at least comprising the following steps:

s11: sterilizing calamus seeds;

s121: sieving river sand with a 16-mesh sieve to obtain fine sand;

s123: sterilizing the culture substrate to obtain a sterilized culture substrate;

s13: artificially inoculating strain on the calamus aseptic seedling;

s132: filling hypha of arbuscular mycorrhizal fungi into the cut, performing dark culture for 7-10 days at the constant temperature of 23-27 ℃, and performing illumination culture for 7-10 days after dark culture;

s2: detecting the inoculation condition of the fungi after culturing for a period of time, picking out and discarding the calamus plants which are not infected by the fungi, and culturing the rest calamus plants to a harvesting period to obtain arbuscular mycorrhizal fungi-calamus symbiont seeds;

s33: sowing arbuscular mycorrhizal fungi-calamus symbiont seeds into the sterilized soil, controlling the distance between the seeds to be 30-35 cm, covering the soil, and compacting;

s4: subsequent weeding and water management.

The formula of the Hoagland's nutrient solution is as follows:

one-half Hoagland's nutrient solution: 472.5mg/L of calcium nitrate, 303.5mg/L of potassium nitrate, 57.5mg/L of ammonium phosphate, 246.5mg/L of magnesium sulfate, 1.75ml/L of iron salt solution and 2.5ml/L of trace element liquid with the mass ratio of 30: 120: 87.

The nutrient solution with 10 times concentration is prepared according to the formula, the solvent is natural water when the nutrient solution is prepared, and the nutrient solution is diluted by the natural water when the nutrient solution is used.

TABLE 1-arbuscular mycorrhizal fungi-Acorus calamus symbiont composition of cultivation raw materials in examples 1-15 and comparative examples 1-3

TABLE 2 cultivation of the mycorrhizal fungi-Acorus calamus symbiont examples 16-18 with comparative example 1

TABLE 3-arbuscular mycorrhizal fungi-Acorus calamus symbiont examples 19-21 and comparative example 3 cultivation raw material composition

TABLE 4 arbuscular mycorrhizal fungi-Acorus calamus symbiont examples 22-26 cultivation raw material composition

Performance test

1. Determination of emergence rate of arbuscular mycorrhizal fungi-calamus symbiont

TABLE 5 influence of different quality of the sterilized culture medium on the rate of emergence of Acorus calamus seeds

	Number of emerged seedlings	Number of seeds sown	Rate of emergence
				Example 1	8 plants	12 granules	0.67
Example 2	11 pieces of grass	12 granules	0.92
				Example 3	12 pieces of grass of Chinese goldthread	12 granules	1
Example 4	11 pieces of grass	12 granules	0.92
				Example 5	12 pieces of grass of Chinese goldthread	12 granules	1
Comparative example 1	0 piece of	12 granules	0

By combining examples 1-5 with comparative example 1 and by combining table 1 and table 5, it can be seen that when the mass of hyphae is 1g and the number of Acorus calamus seeds is 12, the mass of the sterilized culture medium is reduced in a gradient manner compared with 360g, and the emergence rate of Acorus calamus seeds is obviously in a trend of reduction compared with the emergence rate when the mass of the sterilized culture medium is 360 g; when the mass of the sterilization culture substrate is increased in a gradient manner compared with 360g, the emergence rate of the calamus seeds is not changed greatly compared with the emergence rate when the mass of the sterilization culture substrate is 360g, so that when the mass of the hypha is 1g and the number of the calamus seeds is 12, the mass of the sterilization culture substrate is 360g, which is the optimal choice.

TABLE 6 Effect of different amounts of Acorus calamus seeds on the rate of emergence

	Number of emerged seedlings	Number of seeds sown	Rate of emergence
				Example 6	4 pieces of grass of Chinese herbaceous peony	5 granules	0.80
Example 7	7 pieces of Chinese herbal medicine	8 granules	0.88
				Example 3	11 pieces of grass	12 granules	0.92
Example 8	12 pieces of grass of Chinese goldthread	15 granules	0.80
				Example 9	10 pieces of grass of Chinese goldthread	19 granules	0.53

Combining the examples 3 and 6-9, and combining the tables 1 and 6, it can be seen that when the mass of the culture medium is 360g, and the mass of the hyphae is 1g, the sowing amount of the calamus seeds is reduced in a gradient manner compared with 12, and the emergence rate of the calamus seeds is in a reduction trend compared with the emergence rate when the number of the calamus seeds is 12; the sowing amount of the calamus seeds is increased in a gradient way compared with 12 seeds, and the emergence rate of the calamus seeds is in a descending trend compared with the emergence rate when the number of the calamus seeds is 12, so that the number of the calamus seeds is most preferable when the culture medium is 360g in mass and the hypha is 1g in mass.

2. Determination of mycorrhiza infection rate

TABLE 7 influence of arbuscular mycorrhizal fungi on Acorus calamus root infection rates in inoculants of different masses

	Containing spores	Infection rate
			Example 10	1 is provided with	0.28
Example 11	3 are provided with	0.39
			Example 3	6 are	0.64
Example 12	9 are provided with	0.61
			Example 13	12 pieces of	0.57
Comparative example 2	0 number of	0

By combining examples 10-13, example 3 and comparative example 2, and by combining tables 1 and 7, it can be seen that when the mass of the culture medium is 360g and the number of the calamus seeds is 12, the mass of the hyphae is less than 1g, and the rate of the arbuscular mycorrhizal fungi infecting the root system of the calamus is significantly lower than that when the hyphae is 1 g; the mass of the hyphae is more than 1g, and the infection rate of the arbuscular mycorrhizal fungi to the calamus root system is lower than that when the hyphae is 1g, so that when the mass of the culture medium is 360g and the number of the calamus seeds is 12, the hyphae is 1g, which is the optimal choice.

TABLE 8-Effect of sterilized and non-sterilized Medium on the infection Rate of arbuscular mycorrhizal fungi

	Infection rate
		Example 3	0.64
Example 14	0.51
		Example 15	0.57
Comparative example 1	0

In combination with example 3, examples 14 to 15 and comparative example 1, and in combination with tables 2 and 8, it is understood that the rate of infection by arbuscular mycorrhizal fungi cultured on the sterilized culture medium is significantly higher than that cultured on the ordinary culture medium because the ordinary culture medium contains microorganisms such as other fungi and bacteria which compete for nutrients with the arbuscular mycorrhizal fungi, thereby affecting the growth of the arbuscular mycorrhizal fungi and further affecting the rate of infection by arbuscular mycorrhizal fungi, and when the sterilized culture medium is used in combination with the ordinary culture medium, the rate of infection by arbuscular mycorrhizal fungi is also affected.

TABLE 9 influence of different species of arbuscular mycorrhizal fungi on the rate of infection by arbuscular mycorrhizal fungi

	Infection rate
		Example 3	0.64
Example 16	0.45
		Example 17	0.52
Comparative example 2	0

Combining the example 3, the examples 16 to 17 and the comparative example 2, and combining the tables 3 and 9, it can be seen that under the condition that the mass of the culture medium is 360g, the number of the calamus seeds is 12, and the mass of the hypha is 1g, the infection rate of the sacculus moxidensis to the root system of the calamus is the highest, the sacculus tricholobus in the root is the second lowest, the sacculus tricholobus younger than the sacculus tricholobus is the worst, and the sacculus trichoderma moxidensis is the optimal selection under the same conditions.

TABLE 10 influence of different numbers of arbuscular mycorrhizal fungal spores on the rate of arbuscular mycorrhizal fungal infection

	Infection rate
		Example 18	0.45
Example 19	0.53
		Example 3	0.64
Example 20	0.59
		Example 21	0.42

Combining examples 18-21 and 3, and combining table 4 and 10, it can be seen that when the mass of the culture medium is 360g, the number of the calamus seeds is 12, and the mass of the hyphae is 1g, when the number of the arbuscular mycorrhizal fungal spores contained in the inoculant is 5-7 per gram, the infection rate of the arbuscular mycorrhizal fungi is the highest, and when the number of the arbuscular mycorrhizal fungal spores contained in the hyphae is greater than or less than 5-7 per gram, the infection rate of the arbuscular mycorrhizal fungi is reduced, because when the number of the arbuscular mycorrhizal fungal spores is less than 5-7 per gram, the number of the spores is too small, the infection rate is naturally low, and when the number of the arbuscular mycorrhizal fungal spores is greater than 5-7 per gram, the spore density is too large, and the supply of nutrient substances is not in demand, so that the infection rate is reduced.

Example of growth

Examples 2 to 4, examples 7 to 8, examples 11 to 12, examples 14 to 15, examples 16 to 17 and examples 19 to 20 were selected as 13 growth examples, and the plants were grown along the river, and the growth conditions are shown in Table 11.

TABLE 11 growth examples 1-13 plant growth status

	Root state	Plant size
			Growth example 1	General and not robust	Is smaller
Growth example 2	Well-developed and sturdy	Big (a)
			Growth example 3	More developed and stronger	Is larger
Growth example 4	More developed and stronger	Is larger
			Growth example 5	General and not robust	Is smaller
Growth example 6	General and not robust	Is smaller
			Growth example 7	General and not robust	Is smaller
Growth example 8	General and not robust	Is smaller
			Growth example 9	More developed and stronger	Is larger
Growth example 10	More developed and stronger	Is larger
			Growth example 11	Relatively developed and relatively strong	Is larger than
Growth example 12	More developed and stronger	Is larger
			Growth example 13	More developed and stronger	Is larger

Combining examples 2-4, examples 7-8, examples 11-12, examples 14-17, and examples 19-20, and combining growth examples 1-13, it is known that the growth of calamus plants is promoted after the arbuscular mycorrhizal fungi infect the root system of calamus, the root system is developed, the grip ability is strong, and the flood control effect is further improved, and the arbuscular mycorrhizal fungi can improve the soil structure, reduce the loss of soil nutrients, and can act together with the root system of calamus struts to improve the flood control effect. In addition, the absorption capacity of the calamus plants infected by the arbuscular mycorrhizal fungi on nitrogen and phosphorus in water is greatly improved, and the eutrophication of the water body can be improved to a certain extent.

The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications without inventive contribution to the present embodiment as required after reading the present specification, but all of them are protected by patent law within the scope of the present invention.

Claims

1. A method for greening the coastal land of a river is characterized by comprising the following steps:

s1, culturing the arbuscular mycorrhizal fungi-calamus symbiont, which at least comprises the following steps:

s11, sterilizing calamus seeds;

s12, placing the calamus seeds into a culture medium for culturing to obtain calamus aseptic seedlings;

s13, artificially inoculating Calamus aseptic seedlings with bacteria;

s14, cleaning the culture medium of the inoculated root of the Acorus calamus seedlings, transferring the culture medium into a culture dish filled with a sterilized culture medium for culture until the harvest period, placing the culture dish into an incubator with sufficient illumination for culture, and keeping the temperature at 20-25 ℃, wherein the culture conditions are as follows:

s2, culturing for a period of time, detecting the inoculation condition of the fungus, picking out the calamus plants which are not infected by the fungus, discarding, culturing the rest calamus plants to the harvest stage, and obtaining the arbuscular mycorrhizal fungus-calamus symbiont species;

s3, sowing the cultured arbuscular mycorrhizal fungi-calamus symbiont seeds to the river coasts;

s4, subsequent weeding and water management.

2. A method for greening land along a river according to claim 1, wherein the preparation method of the culture medium in S12 comprises: 30g of sterile agar was melted in 1L of sterile water and then cooled to solidify.

3. A method for greening a river riparian land according to claim 1, wherein said artificial inoculation at least comprises the following steps:

s131, vertically cutting the embryonic axis of the calamus aseptic seedling, wherein the incision depth is 2-3 mm;

s132, filling hyphae of the arbuscular mycorrhizal fungi into the cut, performing dark culture for 7-10 days at the constant temperature of 23-27 ℃, and performing light culture for 7-10 days after dark culture.

4. A method for afforesting a riparian region of a river according to claim 1, wherein the arbuscular mycorrhizal fungus is any one of saccharomyces mosseae/saccharomyces youngoticus/saccharomyces intraradicans.

5. A method for greening the riparian land of a river according to claim 1, wherein said culture medium is prepared by the following steps:

s121, screening the river sand through a 16-mesh screen to obtain fine sand;

s122, mixing fine sand and loam according to the mass ratio of 3: 1 to obtain a culture medium;

and S123, sterilizing the culture substrate to obtain a sterilized culture substrate.

6. A method for afforesting a river riparian land according to claim 5, wherein the sterilization treatment is to put the culture substrate into a reaction kettle and maintain the culture substrate for 20min under the conditions of a pressure of 103.4KPa and a temperature of 121 ℃.

7. A method for afforesting a river riparian land according to claim 1, wherein the amount of each raw material is as follows:

sterilizing a culture medium: 160-360 g

The calamus seeds: 5 to 19 particles

Hypha: 0.4 to 2g

A method for greening river riparian lands according to claim 7, wherein the amount of each raw material is preferably as follows:

sterilizing a culture medium: 360g

The calamus seeds: 12 granules

Hypha: 1g

A method as claimed in claim 8, wherein each gram of hyphae contains 5-7 arbuscular mycorrhizal fungi spores.

8. A method for greening river riparian land, according to claim 1, wherein said S3 comprises at least the following steps:

s31, taking the area in front of the arbor along the river as the sowing area of the arbuscular mycorrhizal fungi-calamus symbiont seeds;

s32, deeply ploughing the soil in the planting area, spraying disinfectant for disinfection, and adding coarse sand to improve air permeability;

s33, sowing the arbuscular mycorrhizal fungi-calamus symbiont seeds into the sterilized soil, controlling the distance between the seeds to be 30-35 cm, covering the soil, and compacting.

9. A method for greening land along river banks as claimed in claim 1, wherein said Hoagland's nutrient solution is formulated as follows:

one-half Hoagland's nutrient solution: 472.5mg/L of calcium nitrate, 303.5mg/L of potassium nitrate, 57.5mg/L of ammonium phosphate, 246.5mg/L of magnesium sulfate, 1.75ml/L of iron salt solution and 2.5ml/L of trace element liquid with the mass ratio of 30: 120: 87;

hoagland's nutrient solution with halved phosphorus element: 945mg/L calcium nitrate, 607mg/L potassium nitrate, 115mg/L ammonium phosphate, 493mg/L magnesium sulfate, 2.5ml/L iron salt solution and 5ml/L trace element solution with the mass ratio of 30 parts of urea, 60 parts of sodium dihydrogen phosphate and 87 parts of potassium sulfate;

10. A method for afforesting a river riparian land according to claim 1, wherein said weeding and water and fertilizer management comprises:

and water management, namely watering once a day in the previous week after transplanting, and watering once every three days after one week after transplanting.