CN114767782A

CN114767782A - Jinchuang ointment

Info

Publication number: CN114767782A
Application number: CN202210479932.3A
Authority: CN
Inventors: 张长禄; 王洪
Original assignee: Shengmengtang Hainan Biotechnology Co ltd
Current assignee: Shengmengtang Hainan Biotechnology Co ltd
Priority date: 2022-05-05
Filing date: 2022-05-05
Publication date: 2022-07-22

Abstract

The invention discloses a golden wound ointment, which belongs to the technical field of medicines for trauma, and the ingredients and contents of the golden wound medicine formula of the invention are as follows: 10-15 parts of dragon's blood, 6-8 parts of bulbus fritilariae, 6-8 parts of astragalus membranaceus, 5-6 parts of ginkgo leaves, 2-4 parts of borneol and 0.5-1 part of polyvinylpyrrolidone. The Jinchuang ointment has good curative effect on skin injuries such as incised wound, scratch and the like, and the ointment has strong medicine property stability and long storage life; the Jinchuang ointment of the invention has simple preparation process, less varieties of required medicinal materials and higher economic value.

Description

Jinchuang ointment

Technical Field

The invention relates to a Jinchuang ointment, and belongs to the technical field of trauma medicines.

Background

Trauma is the destruction of tissue or organs by mechanical factors added to the human body. Skin wounds often occur in daily life, and can be caused by incised wounds, scratch wounds, acupuncture and the like. Wounds are not only high in incidence, but also vary widely in degree, and the condition of injury can be severe and complicated, even endangering the lives of the wounded. Severe trauma can cause systemic reactions with localized surface pain, swelling, tenderness, etc. in the wound area.

At present, the ointment for treating skin wounds is various and is divided into traditional Chinese medicines and western medicines, but the ointment has many defects, such as poor curative effect, incapability of adapting to various symptoms, long time for hemostasis and wound healing, adoption of various traditional Chinese medicine components, complex formula, complex preparation process, short storage life, easy failure and the like.

Disclosure of Invention

Aiming at the problems, the invention provides the Jinchuang ointment, which simplifies the formula composition and prolongs the quality guarantee period of the ointment.

In order to achieve the purpose, the Jinchuang ointment provided by the invention comprises the following components in types and contents: 10-15 parts of dragon's blood, 6-8 parts of bulbus fritilariae, 6-8 parts of astragalus membranaceus, 5-6 parts of ginkgo leaves, 2-4 parts of borneol and 0.5-1 part of polyvinylpyrrolidone.

Further, the formula comprises the following components in percentage by weight: 13 parts of dragon's blood, 7 parts of bulbus fritilariae extract, 7 parts of astragalus extract, 5 parts of ginkgo leaf extract, 3 parts of borneol and 0.8 part of polyvinylpyrrolidone.

Further, the preparation method comprises the following steps:

s1, adding the bulbus fritilariae and the astragalus into a container according to the formula amount, adding water with the weight 5-6 times of the weight of the medicines, soaking for 1-2h, decocting for 1-1.5h, cooling to normal temperature, and then primarily filtering through 6-12 layers of medical gauze to obtain filtrate A; adding 2-3 times of water into the residue, decocting for 1 hr, and filtering with 8-10 layers of medical gauze to obtain filtrate B; mixing the filtrate A and B, and concentrating by evaporation under reduced pressure until the water content is less than 5% to obtain Bulbus Fritillariae Cirrhosae and radix astragali extract;

s2, adding folium Ginkgo into a container, adding ethanol 2-3 times of the weight of the medicinal materials, ultrasonic extracting, filtering, and concentrating under reduced pressure to water content less than 5% to obtain folium Ginkgo extract;

s3, pulverizing sanguis Draxonis into powder;

s4, dissolving vaseline in heat-insulating water, adding polyvinylpyrrolidone and folium Ginkgo extract, stirring, adding Bulbus Fritillariae Cirrhosae and radix astragali extract, sanguis Draxonis powder and Borneolum Syntheticum, and stirring to obtain JINCHUANG ointment.

Further, the ultrasonic power of the ultrasonic extraction in the step S2 is 200-.

Further, the application method of the incised wound ointment comprises the steps of cleaning the wound, smearing a proper amount of incised wound ointment to the affected part, and finally covering a layer of medical gauze, wherein the ointment is replaced once a day.

Furthermore, the Jinchuang ointment is used for treating scratch, cut and incised wound.

The Jinchuang ointment has the beneficial effects that:

the Jinchuang ointment has good curative effect on skin injuries such as incised wound, scratch and the like, and the ointment has strong medicine property stability and long storage life; the Jinchuang ointment of the invention has simple preparation process, less varieties of required medicinal materials and higher economic value.

The golden wound ointment of the invention uses dragon's blood, and has the following properties and tastes: sweet in taste; salty; the nature is flat; small toxicity; the Chinese medicinal composition enters heart meridian and liver meridian, and has effects of promoting blood circulation, relieving pain, removing blood stasis, stopping bleeding, promoting granulation, and healing sore; also has certain antifungal effect, and has good effects of stopping bleeding, relieving pain, promoting granulation and healing wound.

The bulbus fritilariae used in the Jinchuang ointment has cool and sweet properties, enters lung channels and stomach channels, has good antibacterial and antihypertensive effects, and has the active component of bulbus fritilariae alkali which is matched with dragon blood for use, so that the effects of better promoting skin regeneration, stopping bleeding and relieving pain can be achieved.

The astragalus root used in the Jinchuang ointment of the invention has sweet and slightly warm nature and taste, enters lung, spleen, liver and kidney meridians, has the functions of enhancing the immunity of the organism, reducing blood pressure and having wider antibacterial action when being used with the invention, and can play better antibacterial and anti-inflammatory roles when being matched with the bulbus fritilariae.

The borneol used in the Jinchuang ointment has pungent, bitter and slightly cold nature and flavor, and enters heart channel, spleen channel and lung channel, has the effects and effects of inducing resuscitation and refreshing mind, clearing heat and relieving pain, and is mainly used for treating fever coma, convulsion, apoplexy, phlegm syncope, qi depression and sudden syncope, central nausea and coma, chest stuffiness and pain, conjunctival congestion, aphtha, sore throat, ear canal purulence and the like. The invention has the effects of clearing heat, relieving pain, promoting granulation and healing sore, and can better promote granulation and healing sore and shorten the course of disease when being matched with the dragon's blood.

The ginkgo leaves used in the incised wound ointment are plants with high medicinal value, and have sweet, bitter, astringent and even properties and taste, and enter heart and lung channels; has effects in promoting blood circulation, dispelling blood stasis, and relieving pain; it contains abundant flavonoids, and also contains a certain amount of catechin, epicatechin, polyphenol, etc.; the components of the invention are matched with each other to play better roles of promoting blood circulation by removing blood stasis, relieving pain and relieving pain.

The polyvinylpyrrolidone used in the incised wound ointment has good dispersion effect, the dragon's blood powder and other medicine extracts can be well dispersed in the vaseline, and the active ingredients of the prepared ointment are uniformly dispersed; the polyvinylpyrrolidone and the ginkgo leaf extract are firstly added into the vaseline, a certain amount of complex can be formed in the vaseline to be dispersed in the mixture by matching the polyvinylpyrrolidone and the ginkgo leaf extract, other medicinal powder or medicinal extract can be mixed with the vaseline and the mixture is stirred uniformly, and then the active ingredients of the medicament can be dispersed in the vaseline, so that the uniform dispersion of the active ingredients can be ensured, the active ingredients can be prevented from coagulating or settling in the long-term storage process, the quality guarantee period of the medicament can be prolonged to a certain extent, and the stability of the efficacy of the active ingredients can be ensured.

The Jinchuang ointment has the advantages of simple preparation process, common types of used medicines, relatively low cost and high economic value.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced otherwise than as specifically described herein, and it will be appreciated by those skilled in the art that the present invention may be practiced without departing from the spirit and scope of the present invention and that the present invention is not limited by the specific embodiments disclosed below.

The Jinchuang ointment comprises the following components in percentage by weight: 10-15 parts of dragon's blood, 6-8 parts of bulbus fritilariae, 6-8 parts of astragalus membranaceus, 5-6 parts of ginkgo leaves, 2-4 parts of borneol and 0.5-1 part of polyvinylpyrrolidone. Preferably, the formula comprises the following components in percentage by weight: 13 parts of dragon's blood, 7 parts of bulbus fritilariae extract, 7 parts of astragalus extract, 5 parts of ginkgo leaf extract, 3 parts of borneol and 0.8 part of polyvinylpyrrolidone.

The preparation method of the incised wound ointment comprises the following steps:

s1, adding the bulbus fritilariae and the astragalus into a container according to the formula amount, adding water with the weight 5-6 times of that of the medicines, soaking for 1-2h, decocting for 1-1.5h, cooling to normal temperature, and then primarily filtering through 6-12 layers of medical gauze to obtain filtrate A; adding 2-3 times of water into the residue, decocting for 1 hr, and filtering with 8-10 layers of medical gauze to obtain filtrate B; mixing the filtrate A and the filtrate B, and concentrating by evaporation under reduced pressure until the water content is less than 5% to obtain Bulbus Fritillariae Cirrhosae and radix astragali extract;

s2, adding folium Ginkgo into a container, adding ethanol 2-3 times of the weight of the medicinal materials, ultrasonic extracting, filtering, and vacuum evaporating to concentrate until the water content is less than 5% to obtain folium Ginkgo extract; preferably, the ultrasonic power is 200-300W, the ultrasonic time is 30-35min, and the temperature is 45-50 ℃.

S3, pulverizing sanguis Draxonis into powder;

The application method of the incised wound ointment comprises cleaning wound, smearing proper amount of incised wound ointment on the affected part, covering with a layer of medical gauze, and changing the ointment once a day. The Jinchuang ointment has good curative effect on common wounds such as metal scratch, cut, stab wound, incised wound and the like.

Example 1

The golden wound ointment comprises the following components in percentage by weight: 10 parts of dragon's blood, 8 parts of bulbus fritilariae, 6 parts of astragalus, 6 parts of ginkgo leaves, 2 parts of borneol and 0.5-1 part of polyvinylpyrrolidone.

s2, adding folium Ginkgo into a container, adding ethanol 2-3 times of the weight of the medicinal materials, ultrasonic extracting, filtering, and concentrating under reduced pressure to water content less than 5% to obtain folium Ginkgo extract; preferably, the ultrasonic power is 200-300W, the ultrasonic time is 30-35min, and the temperature is 45-50 ℃.

S3, pulverizing sanguis Draxonis into powder;

The application method of the incised wound ointment comprises cleaning wound, smearing proper amount of incised wound ointment on the affected part, covering with a layer of medical gauze, and replacing the ointment once every two days. The inventive ointment can be used for treating metal scratch, incised wound, and stab wound.

Example 2

The steps of the embodiment 2 are basically the same as those of the embodiment 1, except that the contents of the components in the formula are different, and the specific embodiment 2 comprises 15 parts by weight of dragon's blood, 6 parts by weight of bulbus fritillariae cirrhosae, 8 parts by weight of astragalus, 5 parts by weight of ginkgo leaves, 4 parts by weight of borneol and 0.5 part by weight of polyvinylpyrrolidone.

Example 3

The steps of the embodiment 3 are basically the same as those of the embodiment 1, except that the contents of the components in the formula are different, and the specific embodiment 3 comprises 13 parts by weight of dragon's blood, 7 parts by weight of bulbus fritillariae cirrhosae extract, 7 parts by weight of astragalus extract, 5 parts by weight of ginkgo leaf extract, 3 parts by weight of borneol and 0.8 part by weight of polyvinylpyrrolidone.

Comparative example 1

Comparative example 1 is a comparative example of example 3, except that polyvinylpyrrolidone is not added to the golden wound ointment, and the procedure is the same as in example 3.

Comparative example 2

Comparative example 2 is a comparative test of example 3, except that ginkgo biloba extract is not added to the wound dressing, and the other is the same as example 3.

Comparative example 3

Comparative example 3 is a comparative test of example 3, except that ginkgo biloba extract and polyvinylpyrrolidone are not added to the woundplast, and the other is the same as example 3.

Test example 1

The golden wound ointments prepared in examples 1-3 and comparative examples 1-3 are checked for microbial indexes according to the general rules 1105, 1106 and 1107 of the Chinese pharmacopoeia 2015 version, and all golden wound ointments meet requirements.

Test example 2

Acute oral toxicity test, the following test was conducted using the golden wound ointments prepared in examples 1 to 3 and comparative examples 1 to 3 as test substances:

selecting 60 Kunming secondary mice with the weight of 18-20g, half male and female, 10 mice in each group of 6 experimental groups, orally administering the test substances once within 24h, wherein the gavage amount is 0.02ml/g BW, the experimental dose is 5000mg/kg BW, observing for 2 weeks and recording related parameters, and after observing for 2 weeks, finding that the test mice of gavage examples 1-3 and comparative examples 1-3 have no toxic expression and death phenomenon, which indicates that the incised wound ointment of the examples and the comparative examples has no toxicity; in addition, the weight gain of the mice in each group has no obvious difference.

Test example 3

Bacterial inhibition test

The test substance: the ointment for treating incised wound of the embodiment 1-3 and the comparative example 1-3 is the ointment for incised wound of the embodiment 3 which is naturally placed for one year.

The test method comprises the following steps: the strain is selected from Escherichia coli and Staphylococcus aureus, and a pre-applied bacteria liquid pouring plate method is adopted. Firstly, pouring about 10ml of sterile LB solid medium into a sterilized clean glass culture dish, and forming a sterile bottom layer after the sterile LB solid medium is coagulated; then, 10ml of LB agar medium with the temperature of about 45 ℃ is taken, and the ratio of the bacterial liquid to the medium is 1: 100 adding proper amount of bacteria liquid (1 x 10)⁸CFU/ml) was added, the bacteria were distributed evenly by gentle shaking, 5ml of the solid culture medium LB containing the bacteria was poured into each dish, and left to stand at room temperature to be cooled and coagulated to serve as an upper layer containing the bacteria. DieThe anti-bacteria effect of the pseudo-incised wound during hemostasis is achieved by preparing a plurality of small paper sheets with the diameter of 6mm through a puncher, then infiltrating 1ml of each group of incised wound ointment into each small paper sheet respectively (specifically, each group of incised wound ointment is poured into a clean culture dish in a proper amount to be spread evenly, then ointment at three positions on the plane of the culture dish is taken to be tested, the incised wound ointment of example 3 placed in a year is placed in a clean container and stored in a sealed mode, ointment at three points above and below is taken to be tested after one year), 0.1mg/ml of ampicillin solution is used for soaking the small paper sheets with the diameter of 6mm serving as positive control, the small paper sheets are added into a marked flat plate, the diameter of a bacteria inhibition ring is measured after 12 hours, three groups are measured in parallel, and the average width of the bacteria inhibition zone is calculated. Similarly, 1ml of sterile aqueous solution and 1ml of 0.1mg/ml of ampicillin solution are respectively soaked in small paper pieces with the diameter of 6mm, the small paper pieces are counted into a prepared flat plate to be used as blank control, the diameter of an inhibition zone is measured after 12 hours, three groups of blank control are parallelly measured, and the average width of an inhibition zone is calculated according to the following calculation formula:

H＝(D-d)/2

in the formula: h is the width of the antibacterial band, D is the outer diameter of the antibacterial band, D is the diameter of the sample, and the unit is mm.

In addition, long-acting observation is carried out on the bacteriostatic effect of each group of incised wound ointment, and the incised wound ointment of the embodiments 1 to 3 and the embodiment 3 placed for one year can still keep the width of a bacteriostatic circle within the observation time of 96 hours, while the incised wound ointment of the comparative examples 1 to 3 has the width of the bacteriostatic circle within the observation time of 96 hours, and especially the golden wound ointment of the comparative example 3 has the large reduction range of the bacteriostatic circle.

TABLE 1 antibacterial activity of various groups of incised wound medicines (unit is mm, bacterial strain is Staphylococcus aureus)

Note: SD refers to the standard deviation of the test results of the three positions in each group of golden wound ointment test, and the average width of the bacteriostatic zone also refers to the average value of the test results of the three positions in each group of golden wound ointment test.

The data in table 1 show that the Jinchuang ointment in each embodiment of the invention has good bacteriostatic effect and is uniformly dispersed, while the Jinchuang ointment in the comparative example has the bacteriostatic activity which is poorer than that in the embodiment at first, and the active drugs in the ointment are not uniformly dispersed, so that the bacteriostatic activity of the ointment at different positions is obviously different.

Test example 4

The blood-stopping test is carried out on the blood,

The test method comprises the following steps:

SD rats were randomly divided into blank control groups (no application of incised wound ointment), the method comprises the following steps of (1) containing a drug experimental group (examples 1-3, comparative examples 1-3 and example 3 of one-year placement), and two groups of positive control groups (alginate hemostatic dressings and standard gauze), wherein 10 drugs in each group are subjected to intraperitoneal injection anesthesia by chloral hydrate, 1/2 are cut off from the tail of a rat, the wound sizes are basically kept consistent, natural bleeding is kept for 15s, the severed wound surface is stretched into a centrifuge tube containing various groups of incised wound drugs (specifically, a proper amount of incised wound ointment in each group is poured into a clean culture dish to be spread, then ointment at three positions on the plane of the culture dish is taken for testing, the incised wound ointment in example 3 of one-year placement is that the ointment is placed in a clean container and is stored in a sealed manner, the ointment at three positions above and below is taken for testing after one year), and the bleeding condition of the wound surface is observed at intervals. Time to hemostasis and blood loss from rats were recorded.

As a result: as can be seen from the data in Table 2, compared with the blank control group and the positive control group, the hemostatic time of the rats containing the drug group in the examples 1-3 is obviously shortened, the blood loss is obviously reduced, the hemostatic effect is good, the hemostatic effect of the ointment for treating the incised wound of the invention after being placed for one year is basically unchanged, and the medicinal effect of the incised wound drug is stable.

Note: table 2 blood stopping time and blood loss are the mean value and standard deviation of the test results of three sites in each group of the golden wound ointment.

Similarly, the hemostasis test also has a result similar to that of the bacteriostasis test, and the data in the table 2 show that the Jinxian ointment in each embodiment of the invention has good hemostasis effect and uniform dispersion of the medicines, while the Jinxian ointment in the comparative example has the hemostasis effect which is slightly lower than that of the embodiment, and the active medicines in the ointment are not uniformly dispersed, so that the hemostasis activity of the ointments at different positions has obvious difference.

Typical cases

Case 1

After a patient visits a woman in 9 years old in 2021 for 10 months, arms of the patient are scratched, and the patient is cleaned, the patient is treated by the gold wound ointment, bleeding is stopped immediately after the gold wound ointment is applied, the gold wound ointment is bound by gauze, the wound is changed once a day, the wound starts to heal after 2 days, the wound forms a scab after 4 days, and the wound heals after 10 days.

Case 2

The ointment is applied to Li Shi, man and Hainan after 2 months of 2022 years, the patients are cut by a knife, the legs are cut, the wounds are cleaned, the bleeding is stopped when the ointment is applied, the wound is bound by gauze, the ointment is changed once a day, the wounds heal after 3 days, the wounds scab after 5 days, the wounds heal after 15 days, and the wounds are infected.

It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Claims

1. The Jinchuang ointment is characterized by comprising the following components in percentage by weight: 10-15 parts of dragon's blood, 6-8 parts of bulbus fritilariae, 6-8 parts of astragalus membranaceus, 5-6 parts of ginkgo leaves, 2-4 parts of borneol and 0.5-1 part of polyvinylpyrrolidone.

2. The Jinchuang ointment according to claim 1, which is characterized in that the types and contents of the components of the formula comprise the following components: 13 parts of dragon's blood, 7 parts of bulbus fritilariae extract, 7 parts of astragalus extract, 5 parts of ginkgo leaf extract, 3 parts of borneol and 0.8 part of polyvinylpyrrolidone.

3. A golden sore ointment according to claim 1 or 2 characterised in that the preparation method comprises the steps of:

s1, adding the bulbus fritilariae and the astragalus into a container according to the formula amount, adding water with the weight 5-6 times of that of the medicines, soaking for 1-2h, decocting for 1-1.5h, cooling to normal temperature, and then primarily filtering through 6-12 layers of medical gauze to obtain filtrate A; adding 2-3 times of water into the residue, decocting for 1 hr, and filtering with 8-10 layers of medical gauze to obtain filtrate B; mixing the filtrate A and B, and concentrating by evaporation under reduced pressure until the water content is less than 5% to obtain Bulbus Fritillariae Cirrhosae and radix astragali extract;

s2, adding folium Ginkgo into a container, adding ethanol 2-3 times of the weight of the medicinal materials, ultrasonic extracting, filtering, and vacuum evaporating to concentrate until the water content is less than 5% to obtain folium Ginkgo extract;

s3, pulverizing sanguis Draxonis into powder;

4. The gold wound ointment as claimed in claim 3, wherein the ultrasonic power of the ultrasonic extraction in step S2 is 200-300W, the ultrasonic time is 30-35min, and the temperature is 45-50 ℃.

5. The golden wound ointment of claim 4, which is used by cleaning a wound, applying a proper amount of golden wound ointment to the affected part, covering a layer of medical gauze, and changing the ointment once a day.

6. The golden wound ointment as claimed in claim 4, which is used to treat scratch, cut and knife wound.