CN114732873A

CN114732873A - Nanometer medicinal preparation for treating toothache and oral ulcer, and its preparation method

Info

Publication number: CN114732873A
Application number: CN202210453367.3A
Authority: CN
Inventors: 蔡建华; 蔡镇宇; 蔡涵宇
Original assignee: Jiangxi Guxiaoning Health Management Co ltd
Current assignee: Jiangxi Guxiaoning Health Management Co ltd
Priority date: 2022-04-27
Filing date: 2022-04-27
Publication date: 2022-07-12

Abstract

The invention belongs to the technical field of medicines, and particularly relates to a nano preparation for treating toothache and oral ulcer and a preparation method thereof, wherein the nano preparation comprises the following active components: nano analgesic, nano therapeutic and nano adjuvant drugs; the mass ratio of the nano analgesic drug, the nano therapeutic drug and the nano auxiliary drug is 1-3:2-4: 3-5. The nanometer preparation prepared by the preparation method of the invention consists of nanometer analgesic drugs, nanometer auxiliary drugs and nanometer therapeutic drugs, can be released in sequence, can realize the purposes of rapidly relieving pain, improving immunity and effectively curing toothache and canker sore diseases caused by internal kidney deficiency, exuberant fire, exuberant stomach fire and the like by strictly controlling the proportion of the nanometer analgesic drugs, the nanometer auxiliary drugs and the nanometer therapeutic drugs, has high pain relieving speed and wound healing speed, difficult relapse and obvious effect.

Description

Nanometer medicinal preparation for treating toothache and oral ulcer, and its preparation method

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to a nano preparation for treating toothache and oral ulcer and a preparation method thereof.

Background

Toothache, one of the most common symptoms of dental diseases in the oral cavity, is manifested by red and swollen gums, pain induced by cold and hot stimulus, swelling of cheeks, etc., and is often caused by infection of dental pulp (dental nerves) due to gingivitis, periodontitis, tooth decay, or broken teeth. The main treatment means is local treatment or systemic medication treatment, but most of the treatments are 'secondary' and cannot be cured radically, and the diseases are easy to relapse.

Oral ulcer, commonly known as "aphtha", is a common ulcerative injury to the oral mucosa, which is usually found in the inner labial, tongue abdomen, buccal mucosa, vestibular sulcus, soft palate, etc., where the mucosa lacks cutinization layer or has poor keratosis. The pain is severe when the oral ulcer is attacked, the local burning pain is obvious, and serious patients can influence diet and speaking, thereby causing great inconvenience to daily life; can be complicated with halitosis, chronic pharyngitis, constipation, headache, dizziness, nausea, debilitation, dysphoria, fever, lymphadenectasis, etc. At present, western medicines are usually applied to the disease clinically to control symptoms, but the disease is difficult to be cured radically, and the western medicines are one of the major problems of the oral diseases.

Although toothache and canker sore seem to be two different diseases, they are oral diseases, which are easy to be complicated, and most of them are caused by kidney deficiency and exuberant fire, and stomach fire. At present, Western medicines such as metronidazole and the like are mainly used for treating toothache caused by kidney deficiency, fire excess and the like in vivo, and although the pain can be quickly relieved, the toothache cannot be radically treated; the oral ulcer is mainly prepared from medicines for diminishing inflammation, relieving pain, promoting healing and the like aiming at the ulcer wound surface, has quick response, treats the symptoms and the root causes, is easy to relapse, and brings repeated pain to patients. Meanwhile, most of the medicines are large in molecular weight, poor in solubility and poor in absorbability, so that the treatment effect is achieved by increasing the dosage, and new problems are brought, such as increasing the irritation to intestines and stomach, increasing toxic and side effects, bringing burden to liver by unabsorbed medicines, reducing immunity, causing low overall utilization rate of the medicines, waste and the like. Therefore, it is necessary to develop an efficient preparation for curing toothache and dental ulcer caused by internal kidney deficiency, exuberance of fire, exuberance of stomach fire and the like.

Disclosure of Invention

The nanometer preparation consists of nanometer analgesic medicine, nanometer auxiliary medicine and nanometer treating medicine in the strictly controllable ratio, and has the functions of fast stopping pain, raising immunity, curing toothache and stomatocace caused by deficiency of the kidney, exuberance of fire, exuberance of stomach fire, etc. and obvious effect.

In order to achieve the above objects, the present invention provides a nano-formulation for treating toothache and oral ulcer, comprising the following active ingredients: nano analgesic, nano therapeutic and nano adjuvant drugs;

the mass ratio of the nano analgesic drug, the nano therapeutic drug and the nano auxiliary drug is 1-3:2-4: 3-5.

The nanometer preparation is a new technology applied to pharmaceutical preparation by nanometer technology, can process substances into nanometer size, and has the advantages of high stability, less irritation to stomach, less toxic and side effects, good solubility, high drug availability, etc. The invention prepares all the medicines into nano grade, can effectively improve the solubility and the utilization rate of the medicines, and can achieve the purposes of quickly relieving pain, improving immunity and effectively solving the problems of toothache and canker sore caused by internal kidney deficiency, exuberance of fire, exuberance of stomach fire and the like by controlling the proportion of the analgesic medicine, the auxiliary medicine and the treatment medicine.

Furthermore, the nanometer analgesic medicine comprises 10-20 parts of frankincense, 10-20 parts of striga asiatica, 15-30 parts of long pepper and 15-30 parts of pepper by weight.

Further, the nano therapeutic medicine comprises 10-15 parts by weight of lotus leaves, 10-15 parts by weight of gardenia, 10-15 parts by weight of honeysuckle, 5-10 parts by weight of dendrobium and 3-6 parts by weight of bezoar.

Further, the auxiliary medicine comprises 5-10 parts of vitamin C, 5-10 parts of vitamin B complex, 5-10 parts of iron agent and 10-20 parts of medlar in parts by weight; the iron agent is amino acid iron. The iron amino acid is stable and easy to be absorbed, and can supplement iron source for organism in time.

The traditional Chinese medicine components used by the invention have the following effects:

frankincense: pungent and bitter with warm nature, entering heart, liver and spleen meridians, and has the actions of regulating qi, promoting blood circulation, relieving pain and removing toxic substance. It is indicated for stagnation of qi and blood, pain in the heart and abdomen, carbuncle, sore, swelling and toxin, traumatic injury, dysmenorrhea, postpartum pain due to blood stasis, etc.

One-row thousand-miles: the nature flavor is bitter, astringent and flat, promotes blood circulation, removes blood stasis, relieves spasm and pain, has the effects of diminishing inflammation, detoxifying, relieving pain, treating lung and relieving cough, is used for treating rheumatic arthralgia, muscle and bone discomfort, sore throat, toothache, abdominal pain and amenorrhea, and is externally used for treating sore, furuncle, pyogenic infections, traumatic injury and the like.

Long pepper pulling: hot, pungent taste; spleen and stomach meridians entered; can be used for treating abdominal pain, emesis, diarrhea, coronary heart disease, angina pectoris, nervous headache and toothache due to stomach cold.

Chinese prickly ash: warming the middle-jiao to dispel cold, removing dampness, relieving pain, killing parasite, and removing fishy smell; has effects in treating food stagnation, abdominal psychroalgia, emesis, hiccup, cough, adverse qi, wind-cold-dampness arthralgia, diarrhea, dysentery, hernia pain, toothache, ascariasis, enterobiasis, pudendal pruritus, sore, and scabies.

Lotus leaf: bitter and astringent taste, slight salty taste, pungent and cool nature, and has effects of clearing summerheat, promoting diuresis, invigorating yang, dispelling blood stasis, stopping bleeding, relieving fever, inhibiting bacteria, and relieving spasm.

Gardenia: bitter and cold in nature; meridian tropism: the heart, lung and triple energizer meridians entered; has effects in purging pathogenic fire, relieving restlessness, clearing away heat, promoting urination, cooling blood, removing toxic substances, tranquilizing mind, stopping bleeding, and relieving swelling, and can be used for treating vexation due to febrile disease, jaundice, dark urine, stranguria with blood, hematemesis, epistaxis, conjunctival congestion, swelling and pain, and pyocutaneous disease due to pathogenic fire; it can be used for treating sprain, contusion and pain.

Honeysuckle flower: is cold in nature and sweet in taste, enters lung, heart and stomach meridians, has the effects of clearing away heat and toxic materials, resisting inflammation, tonifying deficiency and treating wind, and is mainly used for treating diseases such as distending pain, fever due to epidemic febrile disease, toxic heat, carbuncle and ulcer, tumor and the like.

Dendrobe: slightly cold in nature and sweet in taste, entering stomach and kidney meridians; has effects of nourishing yin, clearing heat and lowering fire, and can be used for treating stomach fire, liver fire hyperactivity, yin deficiency, kidney deficiency, sexual impotence, nocturnal emission, soreness of waist and knees, night sweat, low fever, asthenia heat after illness, etc.

Bezoar: fishy smell, sweet taste, cool, heart and liver meridian entered; clearing away heart-fire, eliminating phlegm, inducing resuscitation, cooling liver, extinguishing wind and removing toxicity. Can be used for treating fever unconsciousness, apoplexy with phlegm, convulsive epilepsy, convulsion, epilepsy, mania, sore throat, aphtha of the mouth and tongue, carbuncle, swelling, and furuncle.

Medlar: neutral in nature and sweet in flavor, and enter liver, kidney and lung meridians; has effects of nourishing liver, replenishing kidney essence, moistening lung, and improving immunity and disease resistance.

The selected analgesic drugs are mild in nature and taste, have the antibacterial and anti-inflammatory effects while relieving pain, and can regulate spleen and stomach and improve body functions; the selected therapeutic drugs mainly remove stomach fire, liver fire and the like, purge fire, relieve restlessness, clear away heat and toxic material, resist inflammation and simultaneously can regulate body functions to achieve the aim of deep treatment; the selected auxiliary medicines mainly aim at improving the immunity of the organism, promoting wound healing and blood circulation and improving the health level of the organism.

Further, the particle size of the nano analgesic drug is 50-80 nm; the particle size of the nano therapeutic drug is 120-150 nm; the particle size of the nano auxiliary medicine is 80-100 nm. The invention can keep the stability of each medicine and improve the absorption rate and the utilization rate of the medicine by preparing each medicine into nano level in advance, and the treatment purpose can be achieved by using a small amount of the medicine.

The invention also provides a preparation method of the nanometer preparation for treating toothache and oral ulcer, which comprises the following steps:

s1, preparing a nano analgesic: weighing 10-20 parts of frankincense, 10-20 parts of striga asiatica, 15-30 parts of long pepper and 15-30 parts of pepper in parts by weight, cleaning, baking and frying at 90-110 ℃ for 40-60min, then putting into a grinder to grind to 50-80 meshes, adding 3-5 times of water, boiling for 2-4h, filtering, carrying out steam flow concentration on the filtrate, and then adding the concentrated solution into a high-pressure homogenizer for homogenization for 2-5h to obtain the nano analgesic drug;

s2, preparing a nano auxiliary medicament: weighing 5-10 parts by weight of vitamin C, 5-10 parts by weight of vitamin B complex, 5-10 parts by weight of iron agent and 10-20 parts by weight of medlar, drying medlar, crushing to 60-80 meshes, adding the medlar into a ball mill, adding the vitamin C, the vitamin B complex and the iron agent simultaneously, and carrying out ball milling for 2-3h to obtain a nano auxiliary medicament;

s3, preparation of the nano therapeutic medicine: weighing 10-15 parts of lotus leaf, 10-15 parts of cape jasmine, 10-15 parts of honeysuckle, 5-10 parts of dendrobium and 3-6 parts of bezoar in parts by weight, crushing the lotus leaf, the cape jasmine, the honeysuckle and the dendrobium into 80-100 meshes, leaching for 2-4h by using 75% ethanol with the volume of 2-4 times, and filtering to obtain a leaching solution; pulverizing calculus bovis, adding into ball milling tank, adding above leaching solution, ball milling for 2-4 hr, and evaporating under reduced pressure to obtain nanometer therapeutic medicine;

s4, preparing a nano preparation: the formulation of the nano analgesic drug, nano therapeutic drug and nano adjuvant drug as claimed in claim 1, wherein 2-5% gelatin solution is added into a rapid mixer, nano therapeutic drug is added, stirring is continued for 30-60min, after filtering, standing is carried out for 1-2h, nano adjuvant drug is added, stirring is carried out for 30-60min, after sufficient adhesion, standing is carried out for 1-2h, nano analgesic drug is added, stirring is carried out for 30-60min, and low temperature vacuum drying is carried out to obtain the nano preparation.

The invention firstly prepares the analgesic drug, the auxiliary drug and the therapeutic drug into nano level by different methods, can improve the absorption and utilization rate of each drug, and simultaneously wraps the therapeutic drug with gelatin through later processing, and then sequentially adheres the auxiliary drug and the analgesic drug on the surface of the therapeutic drug, so that the nano preparation can firstly release the analgesic drug, then release the auxiliary drug and finally release the therapeutic drug in the using process, thereby realizing the purposes of quickly relieving pain at fixed points after use, assisting in improving the immunity of the organism, deeply clearing heat and removing toxicity and solving the cause of disease.

Further, in the above technical solution S1, the homogenization pressure is 5 to 12 MPa.

Further, in the technical scheme, in the step S3, the reduced pressure evaporation is carried out until the moisture content of the material is 8-12%, the temperature of the reduced pressure evaporation is 55-90 ℃, and the pressure is 0.04-0.06 MPa.

Further, in the above technique S4, the usage amount of the gelatin solution is 2-3 times of the weight of the nano therapeutic drug, and the rapid stirring speed is 1500-.

Further, in the above technical scheme S4, the low-temperature vacuum drying temperature is 50 to 60 ℃.

The invention has the beneficial effects that:

1. the nano preparation adopts diversified medicines including an analgesic medicine, an auxiliary medicine and a therapeutic medicine, and simultaneously, the proportions of the three medicines are strictly controllable, so that the most direct pain problem can be solved through the analgesic medicine, the immunity of an organism can be improved by utilizing the auxiliary medicine, the health level and the disease resistance of the organism can be improved, the toothache and the oral ulcer diseases caused by the reasons of kidney deficiency, exuberant fire, exuberant stomach fire and the like can be further treated, the relapse is not easy, and the effect is good;

2. the preparation method of the invention prepares the raw materials into nano grade, has small usage amount, good absorption effect, high utilization rate and no toxic or side effect;

3. the preparation method comprises the steps of wrapping therapeutic drugs with gelatin, sequentially adhering auxiliary drugs and analgesic drugs on the surface of the therapeutic drugs to form a capsule-like shape, releasing the analgesic drugs after the therapeutic drugs meet water in the using process, then releasing the auxiliary drugs, and finally releasing the therapeutic drugs.

Detailed Description

The experimental procedures in the following examples are all conventional ones unless otherwise specified. The raw materials in the following examples are all commercially available products and are commercially available, unless otherwise specified.

The present invention is described in further detail below with reference to examples:

example 1

A method for preparing a nanometer medicinal preparation for treating toothache and oral ulcer comprises the following steps:

s1, preparing a nano analgesic: weighing 10 parts by weight of frankincense, 20 parts by weight of striga asiatica, 15 parts by weight of long pepper and 30 parts by weight of pepper, cleaning, baking and frying for 60min at the temperature of 90 ℃, then putting the materials into a grinder to grind to 50-80 meshes, adding 3 times of water, boiling for 2h, filtering, carrying out steam flow concentration on the filtrate, adding the concentrated solution into a high-pressure homogenizer with the pressure of 5MPa, and homogenizing for 5h to obtain the nano analgesic drug;

s2, preparing a nano auxiliary medicament: weighing 5 parts of vitamin C, 10 parts of vitamin B complex, 5 parts of iron agent and 20 parts of medlar in parts by weight, drying the medlar, crushing the dried medlar into 60-80 meshes, adding the crushed medlar into a ball mill, adding the vitamin C, the vitamin B complex and the iron agent, and ball-milling for 2 hours to obtain the nano auxiliary medicament;

s3, preparation of the nano therapeutic medicine: weighing 10 parts of lotus leaf, 15 parts of gardenia, 10 parts of honeysuckle, 10 parts of dendrobium and 3 parts of bezoar according to parts by weight, crushing the lotus leaf, the gardenia, the honeysuckle and the dendrobium into 80-100 meshes, leaching for 4 hours by using 75% ethanol with 2 times of volume, and filtering to obtain a leaching solution; pulverizing calculus bovis, adding into a ball mill, adding the above leaching solution, ball milling for 2 hr, and evaporating under reduced pressure at 90 deg.C and 0.04MPa until water content is 8% to obtain nanometer therapeutic medicine;

s4, preparing a nano preparation: according to the mass ratio of the nano analgesic drug to the nano therapeutic drug to the nano auxiliary drug of 1:4:5, 2% gelatin solution of 2 times of the weight of the nano therapeutic drug is added into a rapid stirrer, the nano therapeutic drug is added, the stirring is continued for 30min, the mixture is filtered by a membrane, and is kept stand for 2h, then the nano auxiliary drug is added, the stirring is carried out for 30min, the mixture is fully adhered, and is kept stand for 1h, finally the nano analgesic drug is added, the stirring is carried out for 30min, and the mixture is dried in a low-temperature vacuum drying oven at the temperature of 50 ℃ to obtain the nano preparation.

Example 2

s1, preparing a nano analgesic: weighing 15 parts by weight of frankincense, 15 parts by weight of striga asiatica, 20 parts by weight of long pepper and 20 parts by weight of pepper, cleaning, baking and frying for 50min at the temperature of 100 ℃, then putting the materials into a grinder to grind to 50-80 meshes, adding 4 times of water, boiling for 3h, filtering, carrying out steam flow concentration on the filtrate, adding the concentrated solution into a high-pressure homogenizer with the pressure of 8MPa, and homogenizing for 3h to obtain the nano analgesic drug;

s2, preparing a nano auxiliary medicament: weighing 8 parts of vitamin C, 7 parts of vitamin B complex, 7 parts of iron agent and 15 parts of medlar in parts by weight, drying the medlar, crushing the dried medlar into 60-80 meshes, adding the crushed medlar into a ball mill, adding the vitamin C, the vitamin B complex and the iron agent simultaneously, and carrying out ball milling for 2.5 hours to obtain the nano auxiliary medicament;

s3, preparation of the nano therapeutic medicine: weighing 12 parts of lotus leaves, 12 parts of gardenia, 12 parts of honeysuckle, 8 parts of dendrobium and 4 parts of bezoar according to parts by weight, crushing the lotus leaves, the gardenia, the honeysuckle and the dendrobium into 80-100 meshes, leaching for 3 hours by using 75% ethanol with the volume being 3 times that of the lotus leaves, the gardenia, the honeysuckle and the dendrobium, and filtering to obtain a leaching solution; pulverizing calculus bovis, adding into a ball mill, adding the above leaching solution, ball milling for 3 hr, and evaporating under reduced pressure at 80 deg.C and 0.05MPa until water content is 10% to obtain nanometer therapeutic medicine;

s4, preparing a nano preparation: according to the mass ratio of the nano analgesic drug to the nano therapeutic drug to the nano auxiliary drug of 2:2:4, firstly adding a 3% gelatin solution 2.5 times the weight of the nano therapeutic drug into a rapid stirrer, adding the nano therapeutic drug, continuously stirring for 40min, filtering a membrane, standing for 1.5h, then adding the nano auxiliary drug, stirring for 40min, fully adhering, standing for 1.5h, finally adding the nano analgesic drug, stirring for 40min, and drying in a low-temperature vacuum drying oven at the temperature of 55 ℃ to obtain the nano preparation.

Example 3

s1, preparing a nano analgesic: weighing 20 parts by weight of frankincense, 10 parts by weight of striga asiatica, 30 parts by weight of long pepper and 15 parts by weight of pepper, cleaning, baking and frying for 40min at the temperature of 110 ℃, then putting the materials into a grinder to grind to 50-80 meshes, adding water with the volume 5 times of that of the materials, boiling for 4h, filtering, carrying out steam flow concentration on the filtrate, adding the concentrated solution into a high-pressure homogenizer with the pressure of 12MPa, and homogenizing for 2h to obtain the nano analgesic drug;

s2, preparing a nano auxiliary medicament: weighing 10 parts of vitamin C, 5 parts of vitamin B complex, 10 parts of iron agent and 10 parts of medlar in parts by weight, drying the medlar, crushing the dried medlar into 60-80 meshes, adding the crushed medlar into a ball mill, adding the vitamin C, the vitamin B complex and the iron agent simultaneously, and carrying out ball milling for 3 hours to obtain a nano auxiliary medicament;

s3, preparation of the nano therapeutic medicine: weighing 15 parts of lotus leaves, 10 parts of gardenia, 15 parts of honeysuckle, 5 parts of dendrobium and 6 parts of bezoar according to parts by weight, crushing the lotus leaves, the gardenia, the honeysuckle and the dendrobium into 80-100 meshes, leaching for 2 hours by using 75% ethanol with 4 times of volume, and filtering to obtain a leaching solution; pulverizing calculus bovis, adding into a ball mill, adding the above leaching solution, ball milling for 4 hr, and evaporating under reduced pressure at 55 deg.C and 0.06MPa until the water content is 12% to obtain nanometer therapeutic medicine;

s4, preparing a nano preparation: according to the mass ratio of the nano analgesic drug to the nano therapeutic drug to the nano auxiliary drug of 3:3:3, firstly adding a gelatin solution with the weight being 3 times of that of the nano therapeutic drug into a rapid stirrer, adding the nano therapeutic drug, continuously stirring for 60min, filtering a membrane, standing for 1h, then adding the nano auxiliary drug, stirring for 60min, fully adhering, standing for 2h, finally adding the nano analgesic drug, performing 30-60min, and drying in a low-temperature vacuum drying oven at the temperature of 60 ℃ to obtain the nano preparation.

Comparative example 1

In S4, the ratio of the nano analgesic drug to the nano therapeutic drug to the nano adjuvant drug is 6:2:4, and the rest method is the same as that of example 2.

Comparative example 2

In S4, the ratio of the nano analgesic drug to the nano therapeutic drug to the nano adjuvant drug is 0.1:2:4, and the rest method is the same as that of example 2.

Comparative example 3

In S4, the ratio of the nano analgesic drug to the nano therapeutic drug to the nano adjuvant drug is 2:6:4, and the rest method is the same as that of example 2.

Comparative example 4

S4, the ratio of the nano analgesic drug to the nano therapeutic drug to the nano adjuvant drug is 2:0.5:4, and the rest method is the same as that of the example 2.

Comparative example 5

In S4, the ratio of the nano analgesic drug to the nano therapeutic drug to the nano adjuvant drug is 2:2:6, and the rest method is the same as that of example 2.

Comparative example 6

In S4, the ratio of the nano analgesic drug to the nano therapeutic drug to the nano adjuvant drug is 2:2:1, and the rest method is the same as that in example 2.

Comparative example 7

A preparation method of a preparation for treating toothache and oral ulcer comprises the following specific steps:

s1, preparing an analgesic: weighing 15 parts by weight of frankincense, 15 parts by weight of striga asiatica, 20 parts by weight of long pepper and 20 parts by weight of pepper, cleaning, baking and frying for 50min at the temperature of 100 ℃, then putting the materials into a grinder to grind to 50-80 meshes, adding 4 times of water, boiling for 3h, filtering, and concentrating the filtrate by steaming to obtain the analgesic;

s2, preparing auxiliary medicines: weighing 8 parts of vitamin C, 7 parts of vitamin B complex, 7 parts of iron agent and 15 parts of medlar in parts by weight, drying the medlar, crushing the dried medlar into 60-80 meshes, adding the crushed medlar into a stirrer, adding the vitamin C, the vitamin B complex and the iron agent simultaneously, and stirring for 2.5 hours to obtain an auxiliary medicament;

s3, preparation of the therapeutic drug: weighing 12 parts of lotus leaves, 12 parts of gardenia, 12 parts of honeysuckle, 8 parts of dendrobium and 4 parts of bezoar according to parts by weight, crushing the lotus leaves, the gardenia, the honeysuckle and the dendrobium into 80-100 meshes, leaching for 3 hours by using 75% ethanol with the volume being 3 times that of the lotus leaves, the gardenia, the honeysuckle and the dendrobium, and filtering to obtain a leaching solution; pulverizing calculus bovis, adding the leaching solution, stirring, and evaporating under reduced pressure at 80 deg.C and 0.05MPa until the water content is 10% to obtain therapeutic medicine;

s4, preparation: adding 3% gelatin solution 2.5 times of the weight of the therapeutic drug into a rapid stirrer according to the mass ratio of 2:2:4 of the analgesic drug, the therapeutic drug and the auxiliary drug, adding the therapeutic drug, continuously stirring for 40min, filtering by a membrane, standing for 1.5h, adding the auxiliary drug, stirring for 40min, fully adhering, standing for 1.5h, adding the analgesic drug, stirring for 40min, and drying in a low-temperature vacuum drying oven at 55 ℃ to obtain the preparation.

Comparative example 8

S4, preparing a nano preparation: mixing the nanometer analgesic medicine, the nanometer therapeutic medicine and the nanometer adjuvant medicine at a mass ratio of 2:2:4, stirring, and drying in a low temperature vacuum drying oven at 55 deg.C to obtain nanometer preparation. The rest of the procedure was the same as in example 2.

And (3) clinical trials:

220 oral patients (110 men and 110 women) aged between 20 and 55 years and continuously suffered from persistent diseases caused by excessive fire due to kidney deficiency, exuberance of stomach fire and the like for more than 2 weeks are recruited, 55 patients suffer from toothache and dental ulcer at the same time, 99 patients suffer from dental ulcer and 66 patients suffer from dental ulcer, the patients are randomly divided into 11 experimental groups, each group comprises 20 patients, each group simultaneously suffers from 5 toothache and dental ulcer, and 9 patients suffer from dental ulcer and 6 toothache, the preparations prepared in the examples 1-3 and the comparative examples 1-8 are respectively adopted for treatment twice a day with the dosage of 0.5g each time, the preparations are respectively used after morning getting up and before night sleep, the treatment is continuously carried out for 15 days, the preparations are not used and then continuously observed for 15 days, and the other patients are all normally taken and eaten. Clinical trial results the healing profile is shown in table 1, and the analgesic effect, the healing profile of the canker sores and the recurrence profile are shown in table 2.

Test investigation indexes are as follows:

(1) healing conditions

And (3) curing: toothache symptom disappears in 1-2 days, oral ulcer heals in 2-4 days, no pain and irritation, good appetite, and no recurrence;

the effect is shown: toothache symptom disappears in 3-5 days, oral ulcer is completely healed in 5-9 days, no pain and irritation are caused, and stomatocace is normal without recurrence;

the method has the following advantages: toothache symptoms disappear in 5-10 days, dental ulcer is basically healed in 10-14 days, pain is relieved, the appetite is normal, and the symptom of relapse exists;

and (4) invalidation: toothache symptoms are not improved and pain is hard to endure, oral ulcer is not healed after 15 days, and the appetite is poor.

TABLE 1 healing conditions

The results in table 1 show that the nano preparation prepared by the nano preparation formula and the preparation method of the embodiments 1-3 of the invention can effectively treat toothache and canker sore diseases caused by kidney deficiency and exuberant fire, exuberant stomach fire and the like, the total effective rate reaches 100%, the cure rate reaches 90.9%, the wound healing speed is high, and no recurrence phenomenon exists in the near term.

The results of the comparative example 2 and the comparative examples 1 to 6 show that the nano preparation obtained by using the nano preparation has the advantages of obviously reduced treatment effect and the most obvious reduction of the number of cured people due to excessive or insufficient analgesic drugs, excessive or insufficient auxiliary drugs and excessive or insufficient treatment drugs, which indicates that the selected drugs are totally effective, but the optimal effect cannot be achieved due to unreasonable proportion, and the rehabilitation speed and the relapse condition are influenced.

The results of the comparative example 2 and the comparative examples 7 to 8 show that the preparation of the traditional Chinese medicine raw materials in the comparative example 7 after direct extraction has the advantages that the total effective rate is obviously reduced compared with the example 2, and the cure rate is 0; comparative example 8, the traditional Chinese medicine components are directly mixed after being prepared into the nano raw materials, and the therapeutic effect of releasing the traditional Chinese medicine components together is not as good as that of example 2, which shows that the preparation method of the invention can improve the absorption and utilization effects of the active ingredients of the medicine.

(2) Analgesic effect, healing of oral ulcer and recurrence

As can be seen from the results in table 2, the nano-preparation prepared by the nano-preparation formulation and the preparation method of the embodiments 1 to 3 of the present invention can relieve pain within 2.5 to 3.3min after use, can completely heal the oral ulcer wound within 4d, and does not recur after drug withdrawal, which indicates that the nano-preparation can rapidly relieve pain and promote wound healing, can effectively solve the symptoms of kidney deficiency, exuberant fire, exuberant stomach fire, etc., of the body, and can improve the disease resistance of the body.

Comparative example 1 has excessive pain-stopping drugs, and although the pain-stopping speed is high, the oral ulcer healing is slow, and the recurrence rate is high; in comparative example 2, the analgesic drug was too little, and although the recurrence rate was relatively low, the analgesic rate was significantly slowed; in comparative example 3, the adjuvant drug was excessive, and although the recurrence rate was relatively low, the analgesic rate and the healing rate of oral ulcer were slow; in comparative example 4, the recurrence rate is obviously increased due to the fact that the auxiliary drugs are too little; comparative example 5, which is overdose with a slow analgesic rate, although the recurrence rate is relatively low and the oral cavity heals faster; in comparative example 6, the pain-relieving time, the oral healing time and the recurrence rate of the drug are obviously increased when the drug is too little; the nano preparation formula can quickly relieve pain and promote wound healing, and can effectively solve the symptoms of toothache and oral ulcer.

In the comparative example 7, the pain relieving time, the oral healing time and the recurrence rate of the traditional Chinese medicine raw materials are obviously increased compared with those of the example 2; the traditional Chinese medicine components in the comparative example 8 are prepared into the nanometer raw materials and then directly mixed, so that the pain relieving time, the oral healing time and the recurrence rate are all improved compared with those of the example 2, but the effect is better compared with that of the comparative example 7, which shows that the nanometer preparation prepared by the preparation method of the invention is easier to be absorbed by organisms, and the absorption effect is better through layered release of the medicine.

In conclusion, the invention strictly controls the formula of each effective component of the nano preparation, and combines the preparation method of the invention, the obtained nano preparation has the total effective rate of 100 percent for treating toothache and oral ulcer diseases caused by internal kidney deficiency, exuberant fire, exuberant stomach fire and other reasons, the pain relieving speed and the wound healing speed are high, the recent relapse is avoided, and the effect is obvious.

Finally, it should be emphasized that the above-described preferred embodiments of the present invention are merely examples of implementations, rather than limitations, and that many variations and modifications of the invention are possible to those skilled in the art, without departing from the spirit and scope of the invention.

Claims

1. A nanometer medicinal preparation for treating toothache and oral ulcer comprises the following active ingredients: nano analgesic, nano therapeutic and nano adjuvant drugs;

2. The nanometer medicinal preparation for treating toothache and oral ulcer, according to claim 1, wherein the nanometer analgesic comprises Olibanum 10-20 parts, herba Senecionis Scandentis 10-20 parts, fructus Piperis Longi 15-30 parts, and fructus Zanthoxyli 15-30 parts.

3. The nano preparation for treating toothache and oral ulcer according to claim 1, wherein the nano treatment medicine comprises 10-15 parts by weight of lotus leaf, 10-15 parts by weight of gardenia, 10-15 parts by weight of honeysuckle, 5-10 parts by weight of dendrobium nobile and 3-6 parts by weight of bezoar.

4. The nano preparation for treating toothache and oral ulcer according to claim 1, wherein the adjuvant comprises 5-10 parts by weight of vitamin C, 5-10 parts by weight of vitamin B complex, 5-10 parts by weight of ferralia, and 10-20 parts by weight of medlar; the iron agent is amino acid iron.

5. The nano preparation for treating toothache and oral ulcer according to claim 1, wherein the nano analgesic has a particle size of 50-80 nm; the particle size of the nano therapeutic drug is 120-150 nm; the particle size of the nano auxiliary medicine is 80-100 nm.

6. The method for preparing the nano preparation for treating toothache and oral ulcer according to any one of claims 1 to 5, comprising the following steps:

s2, preparing a nano auxiliary medicament: weighing 5-10 parts of vitamin C, 5-10 parts of vitamin B complex, 5-10 parts of iron agent and 10-20 parts of medlar in parts by weight, drying the medlar, crushing the medlar into 60-80 meshes, adding the medlar into a ball mill, adding the vitamin C, the vitamin B complex and the iron agent simultaneously, and carrying out ball milling for 2-3h to obtain the nano auxiliary medicament;

s3, preparation of the nano therapeutic medicine: weighing 10-15 parts of lotus leaves, 10-15 parts of gardenia, 10-15 parts of honeysuckle, 5-10 parts of dendrobium and 3-6 parts of bezoar according to parts by weight, crushing the lotus leaves, the gardenia, the honeysuckle and the dendrobium into 80-100 meshes, leaching for 2-4h by using 75% ethanol with the volume of 2-4 times of that of the lotus leaves, the gardenia, the honeysuckle and the dendrobium, and filtering to obtain a leaching solution; pulverizing calculus bovis, adding into ball milling tank, adding above leaching solution, ball milling for 2-4 hr, and evaporating under reduced pressure to obtain nanometer therapeutic medicine;

s4, preparing a nano preparation: the formulation of the nano analgesic drug, nano therapeutic drug and nano adjuvant drug as claimed in claim 1, wherein 2-5% gelatin solution is added into a rapid mixer, nano therapeutic drug is added, stirring is continued for 30-60min, the mixture is allowed to stand for 1-2h after being filtered, then nano adjuvant drug is added, stirring is continued for 30-60min, after being fully adhered, the mixture is allowed to stand for 1-2h, finally nano analgesic drug is added, stirring is continued for 30-60min, and low temperature vacuum drying is carried out to obtain the nano preparation.

7. The method for preparing a nano preparation for treating toothache and oral ulcer according to claim 6, wherein the homogenization pressure in S1 is 5-12 MPa.

8. The method for preparing a nanometer medicinal preparation for treating toothache and oral ulcer according to claim 6, wherein in S3, the evaporation under reduced pressure is carried out until the water content in the material is 8-12%, the temperature of the evaporation under reduced pressure is 55-90 deg.C, and the pressure is 0.04-0.06 MPa.

9. The method for preparing a nanometer medicinal preparation for treating toothache and oral ulcer as claimed in claim 6, wherein in S4, the gelatin solution is used in an amount of 2-3 times the weight of the nanometer medicinal preparation, and the rapid stirring speed is 1500-2500 r/min.

10. The method for preparing a nano-preparation for treating toothache and oral ulcer according to claim 6, wherein the low-temperature vacuum drying temperature in S4 is 50-60 ℃.