CN114703061A

CN114703061A - Be used for cell culture with multi-functional intelligent cell culture dish

Info

Publication number: CN114703061A
Application number: CN202210631927.XA
Authority: CN
Inventors: 张印; 李绍旦; 崔艺馨; 黎发根; 陈琪
Original assignee: 6th Medical Center of PLA General Hospital
Current assignee: 6th Medical Center of PLA General Hospital
Priority date: 2022-06-07
Filing date: 2022-06-07
Publication date: 2022-07-05
Anticipated expiration: 2042-06-07
Also published as: CN114703061B

Abstract

The invention belongs to the technical field of cell culture dish correlation, and particularly relates to a multifunctional intelligent cell culture dish for cell culture. The cell culture dish comprises a base and a mounting plate arranged above the base, wherein a cell adsorption component used for collecting cells and a position adjusting component used for adjusting the position of the cell adsorption component are arranged on the mounting plate, a culture dish body is arranged above the base, a containing groove is formed in the upper portion of the base, the culture dish body is inclined towards the center, a plurality of annular containing plates are fixedly connected inside the culture dish body, baffles are arranged at the inner circle of each annular containing plate, and the bottoms of the annular containing plates are inclined towards the center of the culture dish body. The invention adopts an inclined type containing groove structure, and the annular containing plate and the baffle plate are arranged on the containing groove, so that the volume of the fine reaction solution can be improved, and the simultaneous culture of various tissue cells can be realized.

Description

Be used for cell culture to use multi-functional intelligent cell culture dish

Technical Field

The invention belongs to the technical field of cell culture dish correlation, and particularly relates to a multifunctional intelligent cell culture dish for cell culture.

Background

Cell culture refers to a method of simulating the in vivo environment in vitro, allowing it to survive, grow, reproduce and maintain the main structure and function. Cell culture is an essential process for the whole bioengineering technology. Cell culture itself is a large-scale cloning of cells. The cell culture technology can lead one cell to be cultured into simple single cells or multi-cells with little differentiation in a large quantity. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched. When the cell culture teaching is carried out in a laboratory, the tissue taken by the forceps is mixed with reaction liquid many times, and is placed in a culture dish for aging culture, and after a certain time, the cultured tissue is made into a specimen through a cover plate for microscopic observation.

At present, a tray type structure is adopted by a cell culture dish, the volume of the cell culture dish is limited, and the simultaneous culture of multiple cell types cannot be realized.

In addition, the cell micro-operation technology still depends on the manual operation level, and the requirement on the richness of the manual experience is high. Not only need operating personnel to concentrate on for a long time, also require operating personnel accurate speed and the quantity of controlling the suction cell, operating personnel hand fine jitter can produce the damage to the cell promptly.

Disclosure of Invention

The invention aims to provide a multifunctional intelligent cell culture dish for cell culture, which overcomes the defects that the volume of the culture dish is limited and the types of cultured tissue cells are single in the background technology; the method solves the problems that the cell micromanipulation technology has higher requirement on the abundance of manual experience and is easy to damage cells.

In order to achieve the purpose, the invention provides the following technical scheme:

a multifunctional intelligent cell culture dish for cell culture comprises a base and a mounting plate arranged above the base, wherein a cell adsorption component for collecting cells and a position adjusting component for adjusting the position of the cell adsorption component are arranged on the mounting plate; the culture dish comprises a base and is characterized in that a culture dish body is arranged above the base, a containing groove is obliquely arranged towards the center, a plurality of annular containing plates are fixedly connected inside the culture dish body, baffles are arranged at the inner circle positions of the annular containing plates, and the bottoms of the annular containing plates are obliquely arranged towards the center of the culture dish body.

Preferably, the two sides of the culture dish body are fixedly connected with sliding seats, the inner sides of the sliding seats are slidably connected with sliding blocks, one side of each sliding block is fixedly connected with a first spring, the other end of each first spring is fixedly connected with the inner side of each sliding seat, a cover plate is arranged above the culture dish body, one end of each cover plate is fixedly connected with a shaft rod, the two ends of each shaft rod are rotatably connected with the two sliding blocks respectively, and the other end of each cover plate is fixedly connected with a transmission rope.

Preferably, the cell adsorption component comprises a slide bar, a sealing bearing, a needle tube, a sleeve, a first one-way valve, a second fixing plate, a second spring, a handle, a fixing rod, an arc-shaped plate, an elastic air bag and a second one-way valve, the slide bar is slidably connected to the inside of the center of the mounting plate, a sliding hole matched with the slide bar is formed in the center of the mounting plate, the bottom end of the slide bar is rotatably connected with the needle tube for absorbing cells on the annular containing plate through the sealing bearing, two semicircular through holes are formed in the bottom end of the needle tube, the sleeve is arranged on the outer side of the needle tube, the top of the sleeve is fixedly connected with the outer side of the needle tube, the first one-way valve is installed on the outer wall of the upper side of the sleeve, an air passage communicated with the needle tube is formed in the slide bar, a first vent hole and a second vent hole are formed in the air passage, the top end of the slide bar is fixedly connected with the second fixing plate, the top end of the second fixing plate is fixedly connected with a handle, the outer side of the sliding rod is sleeved with a second spring, two ends of the second spring are respectively contacted with the second fixing plate and the mounting plate but are not fixed, the outer side of the sliding rod is fixedly connected with a fixing rod, the fixing rod is positioned on the lower side of the mounting plate, the other end of the fixing rod is fixedly connected with an arc-shaped plate facing to the side wall of the mounting plate, the outer side of the mounting plate is fixedly connected with four elastic air bags, four first gas channels are arranged in the mounting plate, the elastic air bags and the first gas channels are arranged in a one-to-one correspondence relationship, one end of each first gas channel is communicated with the elastic air bags, the other end of each first gas channel is communicated with a sliding hole in the circle center of the mounting plate, and a second gas channel communicated with the first gas channels is arranged on the mounting plate, the second gas channel is communicated with the outside of the mounting plate, and a second one-way valve is mounted inside the second gas channel.

Preferably, the position adjusting assembly comprises an annular plate fixedly connected to the top end of the mounting plate, at least two support plates and an electric push rod are fixedly connected to the outer side of the annular plate, and each support plate is connected to the bottom end of the corresponding electric push rod.

Preferably, the bottom end of the mounting plate is fixedly connected with two convex blocks, and two sides of each convex block are inclined planes.

Preferably, the outer wall of the slide bar is in sealing contact with the mounting plate.

Preferably, a gap is formed between the inner side of the arc-shaped plate and the side wall of the mounting plate.

Preferably, the first check valve opens in a direction toward the interior of the sleeve.

Preferably, the second check valve opening is directed toward the outside of the mounting plate.

Preferably, this internal cavity structure that is of culture dish, the fixed cover in one side of culture dish body is equipped with the pivot, the both ends of pivot are all rotated and are connected with first fixed plate, two the bottom of first fixed plate all with base upper end fixed connection, bottom one side fixedly connected with balancing weight of culture dish body, the bottom surface of balancing weight and the top surface contact of base, a plurality of automatically controlled valves are installed to the inboard of culture dish body, automatically controlled valve is located two annular and holds the position between the board, the culture dish body orientation the side-mounting of pivot has and is used for carrying out the pipeline that discharges to culture dish body inside liquid, installs the control valve on the pipeline.

Compared with the prior art of cell culture dishes, the invention provides a multifunctional intelligent cell culture dish for cell culture, which has the following beneficial effects:

the invention adopts an inclined type containing groove structure, and the annular containing plate and the baffle plate are arranged on the containing groove, so that the volume of the fine reaction solution can be improved, and the simultaneous culture of various tissue cells can be realized;

by arranging the cell adsorption component, when the cell extraction device works, the bottom end of the needle tube is inserted into liquid of the culture dish body in the vertical direction and is aligned with cells to be collected, and the cell adsorption process and the air adsorption process are alternately performed by rotating the handle, so that the intermittent extraction of the cells is realized, the cells are effectively prevented from being damaged due to improper operation of operators, and the cell extraction efficiency and the safety performance are greatly improved;

thirdly, by arranging the position adjusting assembly, when the cells in the culture dish are collected, the spatial position of the needle tube can be adjusted by moving the roller and adjusting the height of the electric push rod, so that the bottom end of the needle tube is aligned to the position of the cells conveniently, and the operation is simple and convenient;

the invention firstly opens the cover plate and then continues to take up the culture dish by taking up the liquid when the liquid in the culture dish needs to be poured, at the moment, the right end of the cover plate can drive the sliding seat and the culture dish body to rotate clockwise around the rotating shaft, after the sliding seat and the culture dish body rotate for a certain angle, the take-up is stopped, at the moment, the culture dish body is in an inclined state, the electric control valve is opened, the liquid can flow into the interior of the culture dish body through the electric control valve and is discharged from the interior of the culture dish through the pipeline, and meanwhile, the water spraying cleaning can be carried out on the annular containing plates, so that the cleaning effect is better, and the invention has good practical performance.

Drawings

FIG. 1 is a schematic three-dimensional structure of a multifunctional intelligent cell culture dish for cell culture according to the present invention;

FIG. 2 is a schematic top view of a mounting plate according to the present invention;

FIG. 3 is a schematic bottom perspective view of the mounting plate according to the present invention;

FIG. 4 is a schematic diagram of a three-dimensional cross-sectional structure of a mounting plate according to the present invention;

FIG. 5 is an enlarged view of A in FIG. 4;

FIG. 6 is an enlarged view of B in FIG. 4;

FIG. 7 is an enlarged view of C in FIG. 4;

FIG. 8 is an enlarged view of D in FIG. 4;

FIG. 9 is a schematic structural view of a culture dish body and a cover plate according to the present invention;

FIG. 10 is a front view of the culture dish body and the cover plate according to the present invention;

FIG. 11 is a schematic perspective view of a culture dish according to the present invention;

FIG. 12 is a schematic view of a three-dimensional cross-sectional structure of a culture dish according to the present invention.

In the figure: 1. a base; 2. a culture dish body; 3. a rotating shaft; 4. a first fixing plate; 5. a balancing weight; 6. an annular containing plate; 7. a slide base; 8. a cover plate; 9. a slider; 10. a first spring; 11. a driving rope; 12. mounting a plate; 13. a bump; 14. a slide bar; 15. sealing the bearing; 16. a needle tube; 17. a semicircular through hole; 18. a sleeve; 19. a first check valve; 20. a first vent hole; 21. a second vent hole; 22. a second fixing plate; 23. a second spring; 24. a handle; 25. fixing the rod; 26. an arc-shaped plate; 27. an elastic air bag; 28. a first gas passage; 29. a second gas passage; 30. a second one-way valve; 31. an annular plate; 32. a support plate; 33. an electric push rod; 34. a roller; 35. an electrically controlled valve.

Detailed Description

Example 1

The utility model provides a be used for cell culture to use multi-functional intelligent cell culture dish, its includes base 1 and sets up the mounting panel 12 in base 1 top, is provided with the cell adsorption component who is used for collecting the cell on mounting panel 12 and carries out the position control subassembly of adjusting to cell adsorption component position.

A culture dish body 2 is arranged above the base 1. The upper surface of the culture dish body 2 is provided with a containing groove for containing culture solution. The inside fixedly connected with of culture dish body 2 holds board 6 for a plurality of rings shape, the interior round department that the annular held board 6 was equipped with the baffle, and the cross-section that annular held board 6 and baffle is the L shape. The bottom of the annular containing plate 6 is obliquely arranged towards the center of the culture dish body 2.

Put into biological cell and reaction liquid on the board 6 is held to a plurality of rings shape in the culture dish body 2, liquid will be followed and earlier held the board 6 and filled up from the ring shape of top, and then liquid will be followed the edge overflow and flowed to next ring shape and held on the board 6, hold the board 6 and fill up until a plurality of rings shape and hold.

The volume of storing the reaction liquid can be improved by arranging the multilayer annular containing plate 6, the reaction liquid and different histiocytes can be arranged in a layered way, and the number and the types of the culturable histiocytes are increased.

Example 2

This embodiment complements the technical solution of embodiment 1.

One side of the culture dish body 2 is fixedly provided with a rotating shaft 3. Both ends of the rotating shaft 3 are rotatably connected with a first fixing plate 4. The bottom of two first fixed plates 4 all with base 1 upper end fixed connection. The culture dish body 2 can be turned over by the rotating shaft 3.

Be the cavity structure in the culture dish body 2, a plurality of automatically controlled valves 35 are installed to the inboard of culture dish body 2, and automatically controlled valve 35 is located the position between two annular holding plate 6. The electric control valve 35 is used for communicating the internal cavity of the culture dish body 2 with the containing groove.

The bottom surface of one side of pivot 3 is kept away from to the bottom of culture dish body 2 is fixedly connected with balancing weight 5, and the bottom surface of balancing weight 5 and the top surface contact of base 1. Under the effect of balancing weight 5 for placing culture dish body 2 in base 1 upper end that can be stable, keep the horizontality.

A pipe (not shown) for discharging the liquid inside the culture dish body 2 is installed on the right side of the culture dish body 2, and the liquid collected inside the culture dish body 2 is discharged through the pipe. The pipeline is provided with a control valve.

When the liquid in the culture dish body 2 needs to be poured, the balancing weight 5 is lifted, the culture dish body 2 rotates clockwise around the rotating shaft 3, and the culture dish body 2 is in an inclined state. When the electrically controlled valve 35 is opened, the liquid will flow into the interior of the culture dish body 2 through the electrically controlled valve 35. And opens the control valve to be discharged from the inside of the culture dish body 2 through the pipe. The cleaning effect is better by spraying water on the plurality of annular containing plates 6.

Example 3

Referring to fig. 9 to 12, the present embodiment supplements the technical solution of embodiment 2.

The two sides of the culture dish body 2 in the directions of the rotating shaft 3 and the balancing weight 5 are fixedly connected with sliding seats 7. The two sliding seats 7 are connected with sliding blocks 9 in a sliding manner. One side of each of the two sliding blocks 9 is fixedly connected with a first spring 10, and the other end of each first spring 10 is fixedly connected with the inner side of the sliding seat 7. A cover plate 8 is arranged above the culture dish body 2. One end of the cover plate 8 is fixedly connected with a shaft lever. The two ends of the shaft lever are respectively connected with the two sliding blocks 9 in a rotating way. The other end of the cover plate 8 is fixedly connected with a driving rope 11. The other end of the driving rope 11 is convenient for manual operation.

During the use, pulling driving rope 11 receives the line, makes driving rope 11 move along the linear direction as driving rope 11 in fig. 10, makes the right-hand member of apron 8 carry out clockwise around slider 9, and slider 9 can move to the left in slide 7 simultaneously. The cover plate 8 will move leftwards in an inclined state, in the process, the first spring 10 will compress to have elastic potential energy, meanwhile, the right end of the cover plate 8 will sweep the top surface of the culture dish body 2, and the gravity action of the balancing weight 5 can keep the culture dish body 2 in a horizontal state. When the right end of the cover plate 8 moves to the left end of the culture dish body 2, the operation is stopped. At this time, the culture dish body 2 is not covered by the cover plate 8 any more, and at this time, biological cells and reaction liquid can be put on the plurality of annular holding plates 6 in the culture dish body 2.

After all the annular containing plates 6 are filled, the driving rope 11 is paid off, i.e. the driving rope 11 is loosened by hand. Under the elastic action of the first spring 10, the right end of the cover plate 8 will move rightwards and restore the cover plate 8 to the original state again, i.e. the cover plate 8 covers the culture dish body 2. In this process, the right end of the cover plate 8 is linearly swept over the top surface of the culture dish body 2, so that the reaction solution remaining on the top surface of the culture dish body 2 can be scraped off.

When the liquid in the culture dish body 2 needs to be poured, the transmission rope 11 is pulled to take up the liquid. The cover plate 8 is firstly in an open state, then the wire is continuously taken up, and at the moment, the right end of the cover plate 8 can drive the sliding seat 7 and the culture dish body 2 to rotate clockwise around the rotating shaft 3. And stopping taking up after rotating for a certain angle. At this moment the culture dish body 2 is in the tilt state, opens electrically controlled valve 35, and liquid can flow to the inside of culture dish body 2 through electrically controlled valve 35 to discharge from the inside of culture dish body 2 through the pipeline, can carry out the water spray simultaneously and wash on a plurality of annular holding plate 6.

Example 4

Referring to fig. 1 to 8, this embodiment supplements the technical solution of embodiment 1.

The cell adsorbing assembly comprises a sliding rod 14, a sealing bearing 15, a needle tube 16, a sleeve 18, a first one-way valve 19, a second fixing plate 22, a second spring 23, a handle 24, a fixing rod 25, an arc-shaped plate 26, an elastic air bag 27 and a second one-way valve 30.

The mounting plate 12 opens at the center. The slide rod 14 is slidably connected within a slide hole at the center of the mounting plate 12. The bottom end of the sliding rod 14 is rotatably connected with a needle tube 16 which absorbs cells on the annular containing plate 6 through a sealed bearing 15. The bottom end of the needle tube 16 is provided with two semicircular through holes 17. The outside of the needle tube 16 is provided with a sleeve 18, and the top of the sleeve 18 is fixedly connected with the outside of the needle tube 16. The upper outer wall of the sleeve 18 is provided with a first one-way valve 19.

The slide bar 14 is provided with an air passage therein, which is communicated with the needle tube 16, and the air passage is provided with a first vent hole 20 and a second vent hole 21. The top end of the sliding rod 14 is fixedly connected with a second fixing plate 22, and the top end of the second fixing plate 22 is fixedly connected with a handle 24. The outer side of the slide bar 14 is sleeved with a second spring 23. Both ends of the second spring 23 are in contact with the second fixing plate 22 and the mounting plate 12, but are not fixed.

A fixed rod 25 is fixedly connected to the outer side of the sliding rod 14, and the fixed rod 25 is positioned at the lower side of the mounting plate 12. The other end of the fixing rod 25 is fixedly connected with an arc plate 26 facing the side wall of the mounting plate 12. Four elastic airbags 27 are fixedly connected to the outer side of the mounting plate 12. Four first gas channels 28 are opened in the mounting plate 12, the elastic air bags 27 are disposed in a one-to-one correspondence relationship with the first gas channels 28, and one end of each first gas channel 28 is communicated with the elastic air bag 27. The other end of the first gas channel 28 communicates with a sliding hole at the center of the mounting plate 12. The mounting plate 12 is provided with a second gas passage 29 communicated with the first gas passage 28. The second gas channel 29 communicates with the exterior of the mounting plate 12. A second check valve 30 is installed inside the second gas passage 29.

The position adjustment assembly includes an annular plate 31 fixedly attached to the top end of the mounting plate 12. At least two support plates 32 are fixedly connected to the outer side of the annular plate 31. Two support plates 32 are not shown. In this case, the support plate 32 is used only for supporting the cell adsorbing member, but requires human hands for auxiliary support. If the number of the supporting plates 32 is three, the autonomous support can be realized without human assistance.

An electric push rod 33 is fixedly connected to the bottom end of the supporting plate 32, and a roller 34 is mounted at the bottom end of the electric push rod 33. When collecting cells in the culture dish body 2, the spatial position of the needle tube 16 can be adjusted by moving the roller 34 and adjusting the height of the electric push rod 33, so that the bottom end of the needle tube 16 is aligned with the cell position.

It should be noted that two bumps 13 are fixedly connected to the bottom end of the mounting plate 12, and two sides of the two bumps 13 are inclined planes. The two projections 13 correspond to the two oppositely disposed elastic air bags 27, respectively.

The outer wall of the slide 14 abuts the mounting plate 12 to achieve sealing contact. The sliding rod 14 can slide up and down at the center of the mounting plate 12 and can rotate with respect to the mounting plate 12. During the rotation of the sliding rod 14 and the fixing rod 25, the fixing rod 25 will move to the bottom surface of the projection 13, and at this time, the sliding rod 14, the needle tube 16 and the sleeve 18 will move downward for a certain distance as a whole, so that the needle tube 16 and the sleeve 18 will cling to the upper surface of a certain annular containing plate 6.

Further, a certain gap is formed between the inner side of the arc-shaped plate 26 and the outer side of the mounting plate 12, so that the fixing rod 25 can drive the arc-shaped plate 26 to move outside the mounting plate 12 when rotating.

Furthermore, the first non return valve 19 opens into the interior of the sleeve 18 only to allow gas to enter the interior of the sleeve 18 from the exterior of the sleeve 18. The second one-way valve 30 opens in a direction towards the exterior of the mounting plate 12, allowing only gas to pass from the second gas passage 29 to the exterior of the mounting plate 12.

In order to make the elastic air bag 27 capable of recovering after being squeezed, the elastic air bag 27 is made of elastic material and can have the capability of recovering to the original state in an unstressed state.

When tissue cells on a certain annular holding plate 6 of the culture dish body 2 are collected, the spatial position of the needle tube 16 can be adjusted by moving the roller 34 and adjusting the height of the electric push rod 33, so that the bottom end of the needle tube 16 is aligned with the tissue cell position. The bottom end of the needle tube 16 is adjusted to be inserted into the reaction solution of the culture dish body 2 in a vertical direction at a certain distance from the upper surface of the annular holding plate 6 and aligned with the tissue cells to be collected.

At this time, since the needle tube 16 and the sleeve 18 form a sealed space inside, when the needle tube 16 is inserted into the reaction solution, the reaction solution does not automatically enter the needle tube 16 and the sleeve 18, and the reaction solution has a convex liquid level at the bottom ends of the needle tube 16 and the sleeve 18 due to the surface tension of the reaction solution, and the height of the convex liquid level exceeds the position of the semicircular through hole 17.

The handle 24 is manually rotated slowly, and the handle 24 drives the slide rod 14 to rotate through the second fixing plate 22. The sliding rod 14 rotates the arc plate 26 through the fixing rod 25. When the arc plate 26 passes one of the elastic bladders 27 during rotation, the arc plate 26 presses the elastic bladder 27 to compress it. At this time, the elastic air bag 27 does not correspond to the position of the bottom surface of the mounting plate 12 where the projection 13 is provided. The end of the first gas channel 28 near the sliding rod 14 is not vented under the blockage of the sliding rod 14, so that the gas in the elastic air bag 27 is discharged to the outside of the mounting plate 12 through the second check valve 30.

After the arc plate 26 passes through the elastic air bag 27, the elastic air bag 27 is not pressed by the arc plate 26 any more, and the inside of the elastic air bag 27 is in a negative pressure state. After the arc plate 26 continues to rotate by a small angle, the first ventilation hole 20 communicates with the first gas channel 28, and the cells in the culture dish body 2 enter the needle tube 16 under the suction force of the elastic air bag 27. The shaft 14 then continues to rotate and the first vent 20 is again misaligned with the first air passageway 28, at which point the first air passageway 28 is no longer in communication with the needle cannula 16. The interior of the needle cannula 16 is a relatively sealed space in which cells collected in the needle cannula 16 do not move downward due to gravity.

The slide bar 14 and the fixing bar 25 continue to rotate, the fixing bar 25 will reach the bottom surface of the projection 13, at this time, the slide bar 14, the needle tube 16 and the sleeve 18 will move downward for a certain distance, and the bottom ends of the needle tube 16 and the sleeve 18 will just contact with the upper surface of the annular holding plate 6 in the culture dish body 2. The height of the second venting holes 21 is equal to the height of the first gas channels 28.

Then, when the arc-shaped plate 26 passes through the second elastic air bag 27 in the process of continuing to rotate the fixing rod 25, the arc-shaped plate 26 presses the elastic air bag 27 to compress. The second elastic air bag 27 corresponds to the position of the bottom surface of the mounting plate 12 where the projection 13 is provided. The end of the first gas passageway 28 adjacent the plunger 14 is blocked by the plunger 14 and does not communicate with the interior of the needle cannula 16. The gas in the elastic bag 27 is discharged to the outside of the mounting plate 12 through the second check valve 30. After the arc plate 26 passes through the elastic air bag 27, the elastic air bag 27 is not pressed by the arc plate 26 any more, and the inside of the elastic air bag 27 is in a negative pressure state.

After the arc plate 26 continues to rotate by a small angle, the second ventilation hole 21 communicates with the first gas channel 28. Under the suction of the elastic air bag 27, the external air enters the interlayer between the sleeve 18 and the needle tube 16 through the first check valve 19. Since the bottom surfaces of the needle tube 16 and the sleeve 18 are in contact with the upper surface of the annular containing plate 6, the elastic air bag 27 cannot suck the reaction liquid from the annular containing plate 6 even if it has a suction force, and only can suck the gas in the interlayer between the sleeve 18 and the needle tube 16 through the semicircular through hole 17.

It is noted that the sealed bearing 15 is provided for the purpose of: after the bottom surfaces of the needle tube 16 and the sleeve 18 contact the upper surface of the annular containing plate 6, the rotation of the sliding rod 14 can rotate relative to the needle tube 16 due to the arrangement of the sealing bearing 15, and the needle tube 16 is not driven to rotate together, so that the bottom surfaces of the needle tube 16 and the sleeve 18 and the top surface of the annular containing plate 6 are prevented from generating rotational friction.

The rod 14 then continues to rotate and the rod 25 will disengage from the underside of the projection 13. The second vent 21 is again offset in height and rotation from the first air passageway 28, and the first air passageway 28 is no longer in communication with the needle cannula 16.

The above steps are then repeated during rotation of the handle 24. The two processes are alternately performed, namely a cell sucking process and an air sucking process, so that the inside of the needle tube 16 can be alternately subjected to the cell and air sucking process, and the tissue cells can be intermittently sucked.

Example 4

Referring to fig. 1 to 12, this embodiment combines the technical schemes of example 3 and example 4 to obtain a multifunctional cell-only culture dish for cell culture.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a be used for cell culture to use multi-functional intelligent cell culture dish, its includes base (1) and sets up mounting panel (12) above base (1), its characterized in that: a cell adsorption component for collecting cells and a position adjusting component for adjusting the position of the cell adsorption component are arranged on the mounting plate (12);

the culture dish is characterized in that a culture dish body (2) with a containing groove arranged in an inclined mode towards the center is arranged above the base (1), a plurality of annular containing plates (6) are fixedly connected to the inner portion of the culture dish body (2), a baffle is arranged at the inner circle of each annular containing plate (6), and the bottom of each annular containing plate (6) is arranged in an inclined mode towards the center of the culture dish body (2).

2. The multifunctional intelligent cell culture dish for cell culture according to claim 1, wherein: the equal fixedly connected with slide (7) in both sides of culture dish body (2), two the equal sliding connection in inboard of slide (7) has slider (9), two the equal first spring (10) of fixedly connected with in one side of slider (9), the inboard fixed connection of the other one end of first spring (10) and slide (7), the top of culture dish body (2) is equipped with apron (8), the one end fixedly connected with axostylus axostyle of apron (8), the both ends of axostylus axostyle are rotated with two sliders (9) respectively and are connected, the other end fixedly connected with driving rope (11) of apron (8).

3. The multifunctional intelligent cell culture dish for cell culture according to claim 1, wherein: the cell adsorption component comprises a sliding rod (14), a sealing bearing (15), a needle tube (16), a sleeve (18), a first one-way valve (19), a second fixing plate (22), a second spring (23), a handle (24), a fixing rod (25), an arc-shaped plate (26), an elastic air bag (27) and a second one-way valve (30), wherein the sliding rod (14) is connected to the inside of the center of the mounting plate (12) in a sliding manner, a sliding hole matched with the sliding rod (14) is formed in the center of the mounting plate (12), the bottom end of the sliding rod (14) is rotatably connected with the needle tube (16) for absorbing cells on the annular containing plate (6) through the sealing bearing (15), two semicircular through holes (17) are formed in the bottom end of the needle tube (16), the sleeve (18) is arranged on the outer side of the needle tube (16), and the top of the sleeve (18) is fixedly connected with the outer side of the needle tube (16), the outer wall of the upper side of the sleeve (18) is provided with a first one-way valve (19), the inside of the sliding rod (14) is provided with an air passage communicated with the needle tube (16), the air passage is provided with a first vent hole (20) and a second vent hole (21), the top end of the sliding rod (14) is fixedly connected with a second fixing plate (22), the top end of the second fixing plate (22) is fixedly connected with a handle (24), the outer side of the sliding rod (14) is sleeved with a second spring (23), two ends of the second spring (23) are respectively contacted with the second fixing plate (22) and the mounting plate (12) but are not fixed, the outer side of the sliding rod (14) is fixedly connected with a fixing rod (25), the fixing rod (25) is positioned on the lower side of the mounting plate (12), and the other end of the fixing rod (25) is fixedly connected with an arc-shaped plate (26) facing the side wall of the mounting plate (12), four elasticity gasbags (27) of outside fixedly connected with of mounting panel (12), four first gas passage (28) have been seted up to the inside of mounting panel (12), elasticity gasbag (27) are the one-to-one relation setting with first gas passage (28), just the one end and the elasticity gasbag (27) of first gas passage (28) communicate with each other, the other end and the sliding hole of mounting panel (12) centre of a circle department of first gas passage (28) communicate with each other, seted up on mounting panel (12) with communicating second gas passage (29) of first gas passage (28), second gas passage (29) communicate with each other with mounting panel (12) outside, the internally mounted of second gas passage (29) has second check valve (30).

4. The multifunctional intelligent cell culture dish for cell culture according to claim 1, wherein: the position control subassembly includes annular plate (31) fixed connection on mounting panel (12) top the outside fixedly connected with of annular plate (31) has two at least backup pads (32), every the equal fixedly connected with electric putter (33) in bottom of backup pad (32), gyro wheel (34) are installed to the bottom of electric putter (33).

5. The multifunctional intelligent cell culture dish for cell culture according to claim 3, wherein: the bottom fixedly connected with two lugs (13) of mounting panel (12), two the both sides of lug (13) are the inclined plane.

6. The multifunctional intelligent cell culture dish for cell culture according to claim 3, wherein: the outer wall of the sliding rod (14) is in sealing contact with the mounting plate (12).

7. The multifunctional intelligent cell culture dish for cell culture according to claim 3, wherein: a gap is formed between the inner side of the arc-shaped plate (26) and the side wall of the mounting plate (12).

8. The multifunctional intelligent cell culture dish for cell culture according to claim 3, wherein: the first one-way valve (19) opens in a direction towards the interior of the sleeve (18).

9. The multifunctional intelligent cell culture dish for cell culture according to claim 3, wherein: the second check valve (30) opens in a direction toward the exterior of the mounting plate (12).

10. The multifunctional intelligent cell culture dish for cell culture according to claim 1, wherein: be the cavity structure in culture dish body (2), the fixed cover in one side of culture dish body (2) is equipped with pivot (3), the both ends of pivot (3) are all rotated and are connected with first fixed plate (4), two the bottom of first fixed plate (4) all with base (1) upper end fixed connection, bottom one side fixedly connected with balancing weight (5) of culture dish body (2), the bottom surface of balancing weight (5) and the top surface contact of base (1), a plurality of automatically controlled valves (35) are installed to the inboard of culture dish body (2), automatically controlled valve (35) are located two annular and hold the position between board (6), culture dish body (2) orientation the side-mounting of pivot (3) is used for carrying out the pipeline that discharges culture dish body (2) inside liquid, installs the control valve on the pipeline.