CN216337721U

CN216337721U - Full-automatic cell culture device

Info

Publication number: CN216337721U
Application number: CN202123123485.8U
Authority: CN
Inventors: 冯戈
Original assignee: Zhengzhou Semus Biotechnology Co ltd
Current assignee: Zhengzhou Semus Biotechnology Co ltd
Priority date: 2021-12-14
Filing date: 2021-12-14
Publication date: 2022-04-19
Anticipated expiration: 2031-12-14

Abstract

The utility model discloses a full-automatic cell culture device, which comprises a glass box, a carrier assembly, a sealing assembly and a moving assembly, wherein the carrier assembly is arranged on the glass box; glass box: an oxygen storage tank and a carbon dioxide storage tank are fixedly connected to the left side face of the glass box respectively, air outlets at the lower ends of the oxygen storage tank and the carbon dioxide storage tank are communicated with the inside of the glass box through guide pipes, electromagnetic valves are connected in series in the middle of the guide pipes, a vacuum box is arranged at a discharge port arranged on the right side wall of the glass box, and a sampling assembly is arranged on the rear side face of the inside of the glass box; a carrier component: the carrier assembly is arranged on the inner bottom surface of the glass box, and corresponds to the vertical position of the sampling assembly; sealing the assembly: the sealing component is arranged on the right side wall of the interior of the glass box in a matching way; a moving component: set up in the right flank of glass case, remove the subassembly and set up with discharge gate and vacuum box cooperation respectively, this full-automatic cell culture device can sample automatically, reduces the pollution of external environment, keeps the stability of the inside gaseous environment of vacuum box.

Description

Full-automatic cell culture device

Technical Field

The utility model relates to the technical field of cell culture, in particular to a full-automatic cell culture device.

Background

The stem cells are adherent cells, are attached to the surface of the culture dish during culture, can continuously proliferate until the cells are proliferated to be paved on the whole surface of the culture dish, and can be stripped from the culture dish. The stripped stem cells are separated by a centrifuge and other technological processes to obtain cleaner cells which can be cultured in the next generation. The culture dish is taken out from the incubator regularly during stem cell culture, the growth state of cells is observed through a microscope, the cell harvesting time is judged, and the culture quality of the batch of cells is judged, the current cell culture processes are all manual operations, culture solution and the cells are filled into a culture bottle, the culture bottle is placed in the incubator for culture, after the culture is finished, the culture bottle is taken out by an operator and placed under the microscope for observation, after the observation result is recorded, the culture bottle is placed in another cabin by the operator for next operation, the whole process is manual operation, the problems of low efficiency, low cost and easy pollution exist, and further the condition that cell culture fails is caused, therefore, the full-automatic cell culture device is provided.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the defects in the prior art, and provides a full-automatic cell culture device which can automatically sample, reduce the pollution of the external environment, keep the stability of the gas environment in a vacuum box and effectively solve the problems in the background technology.

In order to achieve the purpose, the utility model provides the following technical scheme: a full-automatic cell culture device comprises a glass box, a carrier assembly, a sealing assembly and a moving assembly;

glass box: an oxygen storage tank and a carbon dioxide storage tank are fixedly connected to the left side face of the glass box respectively, air outlets at the lower ends of the oxygen storage tank and the carbon dioxide storage tank are communicated with the inside of the glass box through guide pipes, electromagnetic valves are connected in series in the middle of the guide pipes, a vacuum box is arranged at a discharge port arranged on the right side wall of the glass box, and a sampling assembly is arranged on the rear side face of the inside of the glass box;

a carrier component: the carrier assembly is arranged on the inner bottom surface of the glass box, and corresponds to the vertical position of the sampling assembly;

sealing the assembly: the sealing component is arranged on the right side wall of the interior of the glass box in a matching way;

a moving component: set up in the right flank of glass case, remove the subassembly and set up with discharge gate and vacuum box cooperation respectively.

Wherein: still include the singlechip, the singlechip sets up in the right flank of glass case, and external power source is connected to the input electricity of singlechip, and the input of solenoid valve is connected to the output electricity of singlechip, can take a sample automatically, reduces the pollution of external environment, keeps the stability of the inside gaseous environment of vacuum chamber.

Further, the sampling component comprises a first electric guide rail, a second electric guide rail and an electric pipettor, the first electric guide rail is arranged in the middle of the rear side face of the glass box, the front side face of the rotor seat of the first electric guide rail is provided with the second electric guide rail, the front side face of the rotor seat of the second electric guide rail is provided with the electric pipettor, the input ends of the first electric guide rail, the second electric guide rail and the electric pipettor are electrically connected with the output end of the single chip microcomputer, so that automatic sampling is realized, manual operation is reduced, and pollution is reduced.

Further, the carrier subassembly includes basin, culture dish and resistance area, the basin sets up in the inside of glass case and removes the bottom surface, the culture dish is placed in the upper surface of basin, resistance area array sets up in the inside bottom surface of basin, and the output of singlechip is all connected to the input electricity in resistance area, and the culture dish corresponds with electronic pipettor vertical position, is convenient for keep the invariant of culture temperature.

Further, the removal subassembly includes slip table, motor, gear and rack plate, the slip table is U type structure, and the slip table sets up in the right flank of glass case, the motor sets up in the trailing flank of slip table, and the output shaft of motor rotates with two vertical plate body middle parts of slip table respectively and is connected, and the outer cambered surface middle part of the output shaft of motor is equipped with the gear, the rack plate is connected with the upper surface sliding of slip table through the draw runner that its lower surface symmetry set up, and the rack plate is connected with gear engagement, and the upper surface array of rack plate is equipped with the liquid removal ware, and the liquid removal ware corresponds with the electronic pipettor position, and slip table, motor, gear and rack plate all are located the inside of vacuum chamber, and the rack plate corresponds with the discharge gate position, and the output of singlechip is connected to the input electricity of motor,

further, still include vacuum pump and digital vacuum meter, the vacuum pump sets up in the right flank of glass case, digital vacuum meter sets up in the back lateral wall of vacuum case, the air inlet of vacuum pump and digital vacuum meter all with the inside intercommunication of vacuum case, the output of singlechip is connected to the input electricity of vacuum pump, the input of singlechip is connected to the output electricity of digital vacuum meter, takes out the inside air of vacuum case, reduces the influence of outside gas.

Further, the seal assembly includes L type shrouding, backup pad and electric putter, the backup pad sets up in the inside right wall face of glass case, and the through-hole inner wall fixedly connected with electric putter that the backup pad middle part set up, electric putter's push rod lower extreme fixedly connected with L type shrouding, electric putter's vertical plate body right flank corresponds with the discharge gate position, carries out the shutoff to the discharge gate, avoids the outside air to flow in.

Further, still include gas composition analysis appearance, gas composition analysis appearance sets up in the inside trailing flank of glass case, and the input of singlechip is connected to gas composition analysis appearance's output electricity, monitors glass incasement portion environment, is convenient for adjust glass incasement portion gas content.

Compared with the prior art, the utility model has the beneficial effects that: this full-automatic cell culture device has following benefit:

adding cells to be cultured and a cell culture solution into a culture dish, starting a resistance band at regular time through a single chip microcomputer, electrifying the resistance band to heat water in a water tank, keeping the temperature of the cell culture solution in the culture dish constant, monitoring the content of oxygen and carbon dioxide in a glass box in real time by a gas component analyzer and transmitting a detection value to the single chip microcomputer, controlling an electromagnetic valve connected with an oxygen storage tank to be opened by the single chip microcomputer when the oxygen content is low, filling oxygen into the glass box, filling carbon dioxide into the glass box by opening the electromagnetic valve below the carbon dioxide storage tank when the carbon dioxide content is low, adjusting the proportion of gas in the glass box, starting a vacuum pump through the single chip microcomputer when sampling detection is needed, extracting air in the vacuum box by the operation of the vacuum pump, detecting the vacuum degree in the vacuum box by a digital meter and transmitting the detection value to the single chip microcomputer, when the vacuum degree in the vacuum box reaches a set value, the single chip microcomputer starts an electric push rod, the push rod of the electric push rod upwards pulls an L-shaped sealing plate to upwards move, so that a discharge port is opened, the single chip microcomputer controls a motor to work, an output shaft of the motor drives a gear to rotate, the gear drives a rack plate to move towards the left side through meshing connection with the rack plate, then the single chip microcomputer controls a second electric guide rail and an electric pipettor to work, the second electric guide rail controls the electric pipettor to downwards move, the electric pipettor extracts a sample in the culture dish and moves to the upper side of a pipetting dish under the driving of a first electric guide rail, the electric pipettor releases a cell sample onto the surface of the pipetting dish, then the single chip microcomputer starts the motor to reversely rotate, so that the rack plate moves to the inside of the vacuum box, the L-shaped sealing plate is controlled by the electric push rod to downwards move to close the discharge port, and the pipetting dish is taken out by manually opening an upper cover of the vacuum box, this full-automatic cell culture device can take a sample automatically, reduces the pollution of external environment, keeps the stability of the inside gaseous environment of vacuum chamber.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged schematic view of the structure at A of the present invention.

In the figure: the device comprises a glass box 1, a discharge hole 101, a first electric guide rail 2, a second electric guide rail 3, a single chip microcomputer 4, an oxygen storage tank 5, a carbon dioxide storage tank 6, a carrier component 7, a water tank 71, a culture dish 72, a resistance band 73, a sealing component 8, a sealing plate 81L, a support plate 82, an electric push rod 83, a moving component 9, a sliding table 91, a motor 92, a gear 93, a rack plate 94, a 941 slide bar, an electromagnetic valve 10, an electric pipettor 11, a vacuum box 12, a vacuum pump 13, a digital vacuum meter 14, a gas component analyzer 15 and a liquid transferring dish 16.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-2, the present embodiment provides a technical solution: a full-automatic cell culture device comprises a glass box 1, a carrier component 7, a sealing component 8 and a moving component 9;

glass box 1: the left side surface of the glass box is fixedly connected with an oxygen storage tank 5 and a carbon dioxide storage tank 6 respectively, gas outlets at the lower ends of the oxygen storage tank 5 and the carbon dioxide storage tank 6 are communicated with the inside of the glass box 1 through guide pipes, electromagnetic valves 10 are connected in series in the middle of the guide pipes, a vacuum box 12 is arranged at a discharge port 101 formed in the right side wall of the glass box 1, a sampling assembly is arranged on the rear side surface of the inside of the glass box 1 and comprises a first electric guide rail 2, a second electric guide rail 3 and an electric pipettor 11, the first electric guide rail 2 is arranged in the middle of the rear side surface of the glass box 1, a second electric guide rail 3 is arranged on the front side surface of a rotor base of the first electric guide rail 2, and the electric pipettor 11 is arranged on the front side surface of the rotor base of the second electric guide rail 3, so that sampling is carried out automatically, manual operation is reduced, and pollution is reduced;

wherein: still include singlechip 4, singlechip 4 sets up in the right flank of glass case 1, and external power source is connected to singlechip 4's input electricity, and solenoid valve 10's input is connected to singlechip 4's output electricity, and singlechip 4's output is connected to the equal electricity of input of first electronic guide rail 2, the electronic guide rail 3 of second and electronic pipettor 11.

The carrier component 7: set up in the inside bottom surface of glass case 1, carrier subassembly 7 corresponds with sampling subassembly vertical position, carrier subassembly 7 includes basin 71, culture dish 72 and resistance area 73, basin 71 sets up and removes the bottom surface in the inside of glass case 1, culture dish 72 places in the upper surface of basin 71, resistance area 73 array sets up in the inside bottom surface of basin 71, the equal electricity output of connecting singlechip 4 of input of resistance area 73, culture dish 72 corresponds with 11 vertical position of electronic pipettor, start resistance area 73 regularly through singlechip 4, resistance area 73 circular telegram generates heat the inside water heating of basin 71, make the inside cell culture liquid temperature of culture dish 72 invariable.

The sealing component 8: the sealing component 8 is arranged on the right side wall inside the glass box 1 in a matched mode with the discharge port 101, the sealing component 8 comprises an L-shaped sealing plate 81, a supporting plate 82 and an electric push rod 83, the supporting plate 82 is arranged on the right side wall inside the glass box 1, the inner wall of a through hole formed in the middle of the supporting plate 82 is fixedly connected with the electric push rod 83, the lower end of a push rod of the electric push rod 83 is fixedly connected with the L-shaped sealing plate 81, the right side face of a vertical plate body of the electric push rod 83 corresponds to the discharge port 101 in position, the electric push rod 83 is started by the single chip microcomputer 4, the push rod of the electric push rod 83 upwards pulls the L-shaped sealing plate 81 to move downwards, the discharge port 101 is closed, the discharge port 101 is sealed, and outside air is prevented from flowing into the discharge port 101;

the moving assembly 9: set up in the right flank of glass case 1, removal subassembly 9 sets up with discharge gate 101 and vacuum box 12 cooperation respectively, removal subassembly 9 includes slip table 91, motor 92, gear 93 and rack plate 94, slip table 91 is the U type structure, slip table 91 sets up in the right flank of glass case 1, motor 92 sets up in the trailing flank of slip table 91, the output shaft of motor 92 rotates with two vertical plate body middles of slip table 91 respectively and is connected, motor 92's output shaft extrados surface middle part is equipped with gear 93, rack plate 94 is through the draw runner of its lower surface symmetry setting and the upper surface sliding connection of slip table 91, rack plate 94 is connected with the gear 93 meshing, the upper surface array of rack plate 94 is equipped with liquid-transfering ware 16, liquid-transfering ware 16 corresponds with electronic pipettor 11 position, slip table 91, motor 92, gear 93 and rack plate 94 all are located the inside vacuum box 12, rack plate 94 corresponds with discharge gate 101 position, the input end of the motor 92 is electrically connected with the output end of the single chip microcomputer 4, the single chip microcomputer 4 controls the motor 92 to work, the output shaft of the motor 92 drives the gear 93 to rotate, and the gear 93 is meshed with the rack plate 94 to drive the rack plate 94 to move towards the left side.

The vacuum pump 13 is arranged on the right side face of the glass box 1, the digital vacuum meter 14 is arranged on the rear side wall of the vacuum box 12, air inlets of the vacuum pump 13 and the digital vacuum meter 14 are communicated with the inside of the vacuum box 12, an input end of the vacuum pump 13 is electrically connected with an output end of the single chip microcomputer 4, an output end of the digital vacuum meter 14 is electrically connected with an input end of the single chip microcomputer 4, the vacuum pump 13 is started through the single chip microcomputer 4, the vacuum pump 13 works to extract air in the vacuum box 12, the digital vacuum meter 14 detects the vacuum degree in the vacuum box 12 and transmits a detection value to the single chip microcomputer 4, the air in the vacuum box 12 is extracted, and influences of external air are reduced.

Wherein: still include gas composition analysis appearance 15, gas composition analysis appearance 15 sets up in glass case 1's inside trailing flank, and singlechip 4's input is connected to gas composition analysis appearance 15's output electricity, and inside gas composition analysis appearance 15 real-time supervision glass case 1 inside oxygen and carbon dioxide's content and will detect numerical value and carry to singlechip 4, monitors glass case 1 internal environment, is convenient for adjust glass case 1 inside gas content.

The working principle of the full-automatic cell culture device provided by the utility model is as follows:

adding cells to be cultured and a cell culture solution into a culture dish 72, starting a resistance band 73 at regular time through a single chip microcomputer 4, electrifying the resistance band 73 to generate heat to heat water in a water tank 71 so that the temperature of the cell culture solution in the culture dish 72 is constant, monitoring the content of oxygen and carbon dioxide in a glass box 1 in real time by a gas component analyzer 15 and transmitting a detection value to the inside of the single chip microcomputer 4, controlling an electromagnetic valve 10 connected with an oxygen storage tank 5 to be opened by the single chip microcomputer 4 when the content of the oxygen is low, filling the oxygen into the glass box 1, filling the carbon dioxide into the glass box 1 by opening the electromagnetic valve 10 below a carbon dioxide storage tank 6 when the content of the carbon dioxide is low, thereby adjusting the proportion of gas in the glass box 1, starting a vacuum pump 13 through the single chip microcomputer 4 when sampling detection is needed, and extracting the air in a vacuum box 12 by the operation of the vacuum pump 13, the digital vacuum meter 14 detects the vacuum degree inside the vacuum box 12 and transmits the detected value to the single chip microcomputer 4, when the vacuum degree inside the vacuum box 12 reaches a set value, the single chip microcomputer 4 starts the electric push rod 83, the push rod of the electric push rod 83 pulls the L-shaped sealing plate 81 upwards to move, the discharge port 101 is opened, the single chip microcomputer 4 controls the motor 92 to work, the output shaft of the motor 92 drives the gear 93 to rotate, the gear 93 drives the rack plate 94 to move towards the left side through being meshed with the rack plate 94, then the single chip microcomputer 4 controls the second electric guide rail 3 and the electric pipettor 11 to work, the second electric guide rail 3 controls the electric pipettor 11 to move downwards, the electric pipettor 11 extracts the sample inside the culture dish 72 and moves to the upper part of the pipetting dish 16 under the driving of the first electric guide rail 2, the electric pipettor 11 releases the cell sample to the surface of the pipetting dish 16, then the single chip microcomputer 4 starts the motor 92 to rotate reversely, the rack plate 94 is moved into the vacuum chamber 12, the L-shaped sealing plate 81 is controlled by the electric push rod 83 to move downwards to close the discharge port 101, and the upper cover of the vacuum chamber 12 is manually opened to take out the pipette 16.

It should be noted that the first electric rail 2, the second electric rail 3, the electric pipettor 11, the resistance band 73, the electric push rod 83, the motor 92, the electromagnetic valve 10, the vacuum pump 13, the digital vacuum meter 14, and the gas composition analyzer 15 disclosed in the above embodiments may be freely configured according to practical application scenarios, the electric pipettor 11 is proposed to be an E4 XLS + multi-channel electric pipettor, the vacuum pump 13 may be an HP-0200V oil-free silent vacuum pump, the gas composition analyzer 15 may be an NK-402B multi-component gas analyzer, and the single chip 4 controls the first electric rail 2, the second electric rail 3, the electric pipettor 11, the resistance band 73, the electric push rod 83, the motor 92, the electromagnetic valve 10, the vacuum pump 13, the digital vacuum meter 14, and the gas composition analyzer 15 to operate by using a method commonly used in the prior art.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. The utility model provides a full-automatic cell culture device which characterized in that: comprises a glass box (1), a carrier component (7), a sealing component (8) and a moving component (9);

glass box (1): the left side surface of the glass box is fixedly connected with an oxygen storage tank (5) and a carbon dioxide storage tank (6) respectively, air outlets at the lower ends of the oxygen storage tank (5) and the carbon dioxide storage tank (6) are communicated with the inside of the glass box (1) through guide pipes, electromagnetic valves (10) are connected in series in the middle of the guide pipes, a vacuum box (12) is arranged at a discharge port (101) formed in the right side wall of the glass box (1), and a sampling assembly is arranged on the rear side surface of the inside of the glass box (1);

carrier assembly (7): the carrier assembly (7) is arranged on the inner bottom surface of the glass box (1) and corresponds to the vertical position of the sampling assembly;

closure assembly (8): the sealing component is arranged on the right side wall of the interior of the glass box (1), and the sealing component (8) is matched with the discharge hole (101);

moving assembly (9): the moving component (9) is arranged on the right side surface of the glass box (1) and is respectively matched with the discharge hole (101) and the vacuum box (12);

wherein: the glass box is characterized by further comprising a single chip microcomputer (4), wherein the single chip microcomputer (4) is arranged on the right side face of the glass box (1), an external power supply is electrically connected with the input end of the single chip microcomputer (4), and the input end of the electromagnetic valve (10) is electrically connected with the output end of the single chip microcomputer (4).

2. The fully automated cell culture apparatus of claim 1, wherein: the sampling assembly comprises a first electric guide rail (2), a second electric guide rail (3) and an electric pipettor (11), the first electric guide rail (2) is arranged in the middle of the rear side face of the glass box (1), the front side face of the rotor seat of the first electric guide rail (2) is provided with the second electric guide rail (3), the front side face of the rotor seat of the second electric guide rail (3) is provided with the electric pipettor (11), and the input ends of the first electric guide rail (2), the second electric guide rail (3) and the electric pipettor (11) are electrically connected with the output end of the single chip microcomputer (4).

3. The fully automated cell culture apparatus of claim 2, wherein: carrier subassembly (7) include basin (71), culture dish (72) and resistance area (73), basin (71) set up in the inside bottom surface of glass case (1), the upper surface in basin (71) is placed in culture dish (72), resistance area (73) array sets up in the inside bottom surface of basin (71), and the output of singlechip (4) is all connected to the input of resistance area (73) electricity, and culture dish (72) correspond with electronic pipettor (11) vertical position.

4. The fully automated cell culture apparatus of claim 2, wherein: the movable assembly (9) comprises a sliding table (91), a motor (92), a gear (93) and a rack plate (94), the sliding table (91) is of a U-shaped structure, the sliding table (91) is arranged on the right side face of the glass box (1), the motor (92) is arranged on the rear side face of the sliding table (91), an output shaft of the motor (92) is respectively connected with the middle parts of two vertical plate bodies of the sliding table (91) in a rotating manner, the gear (93) is arranged in the middle part of the outer arc face of the output shaft of the motor (92), the rack plate (94) is connected with the upper surface of the sliding table (91) in a sliding manner through sliding strips symmetrically arranged on the lower surface of the rack plate (94), the rack plate (94) is connected with the gear (93) in a meshing manner, a liquid transfer vessel (16) is arranged on the upper surface of the rack plate (94), the liquid transfer vessel (16) corresponds to the position of the electric liquid transfer vessel (11), and the sliding table (91), the motor (92), the gear (93) and the rack plate (94) are all located inside the vacuum box (12), the rack plate (94) corresponds to the discharge hole (101), and the input end of the motor (92) is electrically connected with the output end of the singlechip (4).

5. The fully automated cell culture apparatus of claim 1, wherein: still include vacuum pump (13) and digital vacuum meter (14), vacuum pump (13) set up in the right flank of glass case (1), digital vacuum meter (14) set up in the back lateral wall of vacuum case (12), and the air inlet of vacuum pump (13) and digital vacuum meter (14) all communicates with the inside of vacuum case (12), and the output of singlechip (4) is connected to the input electricity of vacuum pump (13), and the input of singlechip (4) is connected to the output electricity of digital vacuum meter (14).

6. The fully automated cell culture apparatus of claim 1, wherein: seal subassembly (8) including L type shrouding (81), backup pad (82) and electric putter (83), backup pad (82) set up in the inside right wall of glass case (1), through-hole inner wall fixedly connected with electric putter (83) that backup pad (82) middle part set up, the push rod lower extreme fixedly connected with L type shrouding (81) of electric putter (83), the vertical board body right flank of electric putter (83) corresponds with discharge gate (101) position.

7. The fully automated cell culture apparatus of claim 1, wherein: the glass box is characterized by further comprising a gas component analyzer (15), wherein the gas component analyzer (15) is arranged on the inner rear side face of the glass box (1), and the output end of the gas component analyzer (15) is electrically connected with the input end of the single chip microcomputer (4).