CN220745855U

CN220745855U - Kit capable of rapidly separating and extracting dental pulp stem cells

Info

Publication number: CN220745855U
Application number: CN202322279104.8U
Authority: CN
Inventors: 胡树波; 葛婷婷; 李娟�
Original assignee: Hangzhou Tongce Biotechnology Co ltd
Current assignee: Hangzhou Tongce Biotechnology Co ltd
Priority date: 2023-08-24
Filing date: 2023-08-24
Publication date: 2024-04-09
Anticipated expiration: 2033-08-24

Abstract

The utility model belongs to the technical field of dental pulp stem cell separation and extraction, and particularly relates to a kit capable of rapidly separating and extracting dental pulp stem cells, which comprises the following components: a kit body, a control panel, and an endodontic inlet; the top of the reagent box body positioned on the same straight line direction of the dental pulp input port is provided with a flushing liquid box, a complete culture medium liquid box I, an air cylinder and a complete culture medium liquid box II at intervals, one side of the metal culture dish is connected with a power mechanism for driving the metal culture dish to move along the length direction of the reagent box body, the bottom of the flushing liquid box is connected with a flushing nozzle, the culture medium liquid output dropper is connected with the bottom of the complete culture medium liquid box I, the bottom of the cylinder is connected with the grinding plate, the bottom of the complete culture medium liquid box II is communicated with the incubation incubator, and the kit can automatically perform a series of operations on dental pulp, so that the rapid and efficient separation and extraction functions of dental pulp stem cells are realized.

Description

Kit capable of rapidly separating and extracting dental pulp stem cells

Technical Field

The utility model belongs to the technical field of dental pulp stem cell separation and extraction, and particularly relates to a kit capable of rapidly separating and extracting dental pulp stem cells.

Background

Dental pulp stem cells are derived from children's deciduous teeth, adult's wisdom teeth and orthodontic teeth, and are stored as they decrease in biological activity with age. The dental pulp stem cells are collected and required to be extracted in an oral cavity hospital, the extracted teeth are placed in a special tooth transfer box and sent to a laboratory within 48 hours for professional operations such as extraction, detection, preparation and the like, and finally frozen in liquid nitrogen at the temperature of minus 196 ℃, and when the dental pulp stem cells are extracted, special measures are required to be taken to avoid infection, particularly strict aseptic measures are required to be taken in the dental pulp extraction process, particularly in the dental pulp treatment process, so that dental pulp is not polluted.

When the existing dental pulp stem cells are extracted, the dental pulp stem cells are generally classified and extracted through manual operation, namely by using a culture medium, a culture dish, corresponding test tubes, a clamp and other equipment, and through carefully and carefully operating by an operator, the dental pulp stem cells can be accurately extracted to the required dental pulp stem cells in a certain period of time, but along with the continuous progress of the technology and the increase of the storage quantity scale of the dental pulp stem cells by people, the simple manual operation is often difficult to quickly and slowly meet the requirements of mass dental pulp stem cell separation and extraction;

therefore, in view of the above problems, the present technical solution proposes a kit capable of rapidly separating and extracting dental pulp stem cells.

Disclosure of Invention

The embodiment of the utility model aims to provide a kit capable of rapidly separating and extracting dental pulp stem cells, and aims to solve the problems.

The utility model is realized in such a way that a kit for rapidly separating and extracting dental pulp stem cells comprises: a kit body, a control panel, and an endodontic inlet; the dental pulp input port is arranged on one side of the top of the reagent box body, the top of the reagent box body positioned on the same straight line direction of the dental pulp input port is provided with a flushing liquid box, a complete culture medium liquid box I, a cylinder and a complete culture medium liquid box II at intervals, the interior of the reagent box body positioned right below the dental pulp input port is circularly moved along the directions of the flushing liquid box, the complete culture medium liquid box I, the cylinder and the complete culture medium liquid box II to be provided with a metal culture dish, the top of the metal culture dish is provided with a culture dish groove with a circular structure, one side of the metal culture dish is connected with a power mechanism for driving the metal culture dish to move along the length direction of the interior of the reagent box body, the dental pulp is input into the culture dish groove along the dental pulp input port through the dental pulp, and then is sequentially transferred to the lower parts of the flushing liquid box, the complete culture medium liquid box I and the complete culture medium liquid box II under the driving of the power mechanism, the bottom of the flushing liquid tank is connected with a flushing nozzle, the cell cleaning liquid in the flushing liquid tank is flushed towards the dental pulp in the culture dish tank through the flushing nozzle, pollution is reduced, the bottom of the complete culture medium tank I is connected with a culture medium liquid output dropper, the culture medium liquid output dropper is used for inputting the culture medium liquid in the complete culture medium tank I into the culture dish tank, immersing the dental pulp, the bottom of the air cylinder is connected with a grinding plate, the air cylinder controls the grinding plate to circularly lift and fall to grind the dental pulp in the culture dish tank to form dental pulp tissue, the bottom of the complete culture medium tank II is communicated with an incubation incubator, the dental pulp tissue after grinding enters the incubation incubator, the dental pulp tissue is incubated in the incubation incubator, the culture medium liquid is replaced until the dental pulp stem cells with required content are cultured, thereby realizing the automatic separation and extraction function of the dental pulp stem cells.

According to the kit capable of rapidly separating and extracting dental pulp stem cells, provided by the utility model, the power mechanism is arranged in the kit body to control the metal culture dish to move, and then the metal culture dish is placed in the flushing nozzle, the culture medium liquid output dropper, the grinding plate and the incubation incubator to perform a series of dental pulp treatment, and the whole process is automatically operated to flush, soak, grind and culture dental pulp, so that stem cells in dental pulp can be rapidly separated and extracted, manual participation is reduced, and extraction efficiency is improved.

Drawings

Fig. 1 is a schematic diagram showing the structure of a kit for rapidly separating and extracting dental pulp stem cells at a first visual angle.

Fig. 2 is a schematic diagram of a second visual angle structure of a kit for rapidly separating and extracting dental pulp stem cells.

Fig. 3 is a schematic diagram showing the structure of a third visual angle of a kit for rapidly separating and extracting dental pulp stem cells.

Fig. 4 is a schematic diagram showing the internal structure of a kit for rapidly separating and extracting dental pulp stem cells in front view.

Fig. 5 is an enlarged schematic view of the structure of fig. 3C.

Fig. 6 is an enlarged schematic view of the structure of fig. 4 a.

FIG. 7 is a schematic diagram showing the structure of the side wall of an incubator in a kit for rapid isolation and extraction of dental pulp stem cells.

In the accompanying drawings: the kit comprises a kit body 10, a control panel 11, a dental pulp input port 12, a servo motor 13, a nut 14, a screw rod 15, a flushing liquid tank 16, a complete medium liquid tank I17, a complete medium liquid tank II 18, a flushing nozzle 19, a medium liquid output dropper 20, a grinding plate 21, an air cylinder 22, an incubation incubator 23, a connecting rod 24, a metal culture dish 25, a culture dish groove 26, a sliding groove 27, a sliding rail 29, a top column 30, a liquid suction pipe 32, a pump body 33, an electric lifting rod 34, a liquid discharge pipe 35, a waste liquid storage bin 36, an elastic rubber baffle 37, a connecting rod slideway 38, a split sealing retainer ring 39 and a sealing block 41.

Detailed Description

The present utility model will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present utility model more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.

Specific implementations of the utility model are described in detail below in connection with specific embodiments.

As shown in fig. 1 to 4, a structure diagram of a kit for rapidly separating and extracting dental pulp stem cells according to an embodiment of the present utility model includes: a kit body 10, a control panel 11, and an dental pulp input port 12; the dental pulp input port 12 is arranged on one side of the top of the reagent box body 10, a flushing liquid tank 16, a complete medium liquid tank I17, an air cylinder 22 and a complete medium liquid tank II 18 are arranged on the top of the reagent box body 10 on the same straight line direction of the dental pulp input port 12 at intervals, a metal culture dish 25 is circularly arranged in the reagent box body 10 right below the dental pulp input port 12 along the flushing liquid tank 16, the complete medium liquid tank I17, the air cylinder 22 and the complete medium liquid tank II 18, a culture dish groove 26 with a circular structure is arranged on the top of the metal culture dish 25, a power mechanism for driving the metal culture dish 25 to move along the length direction of the interior of the reagent box body 10 is connected on one side of the metal culture dish 25, the dental pulp is input into the culture dish groove 26 along the dental pulp input port 12, and then driven by the power mechanism, the method comprises sequentially transferring to a flushing liquid tank 16, a complete medium liquid tank I17 and a complete medium liquid tank II 18, connecting a flushing nozzle 19 to the bottom of the flushing liquid tank 16, flushing cell cleaning liquid in the flushing liquid tank 16 toward dental pulp in a culture dish groove 26 by the flushing nozzle 19, reducing pollution, connecting a medium liquid output dropper 20 to the bottom of the complete medium liquid tank I17, inputting medium liquid in the complete medium liquid tank I17 into the culture dish groove 26 by the medium liquid output dropper 20, soaking dental pulp, connecting a grinding plate 21 to the bottom of a cylinder 22, controlling the grinding plate 21 to circularly lift and fall by the cylinder 22 to grind dental pulp in the culture dish groove 26 to form dental pulp tissue, connecting an incubation culture tank 23 to the bottom of the complete medium liquid tank II 18, incubating the dental pulp tissue after grinding into the incubation culture tank 23, incubating the dental pulp tissue in the incubation culture tank 23, and replacing the culture medium until the dental pulp stem cells with the required content are cultured, thereby realizing the automatic separation and extraction function of the dental pulp stem cells.

In the embodiment of the utility model, one side of the top of the kit body 10 is provided with a control panel 11, the control panel 11 is used for controlling the start and stop of all electric power components in the kit body 10, the tops of a flushing fluid tank 16, a complete medium fluid tank I17 and a complete medium fluid tank II 18 are respectively communicated with a solution inlet upwards and are used for supplying corresponding internal solutions, wherein a cell cleaning solution is arranged in the flushing fluid tank 16 and is used for safely and fully cleaning dental pulp, and a culture medium fluid is arranged in the complete medium fluid tank I17 and the complete medium fluid tank II 18 and is used for keeping the survival and the culture of cells; in order to maintain the tightness of the inside of the kit body 10, sealing plates are arranged at the dental pulp input port 12 and the solution inlet port to prevent external dust, bacteria and the like from entering the inside of the kit body 10;

specifically, the power mechanism comprises a screw rod 15 rotatably arranged at one side inside the kit body 10, one end of the screw rod 15 is connected with a servo motor 13, the other end of the screw rod 15 is rotatably connected to the inner wall of the kit body 10 through a bearing, a nut 14 is connected to the screw rod 15 in a threaded manner, a guiding device for limiting the screw rod to rotate is arranged on the nut 14, the servo motor 13 is electrically connected with the control panel 11, namely, the servo motor 13 is started to drive the screw rod 15 to rotate, then the nut 14 is controlled to move on the screw rod 15, one side of the nut 14, facing the metal culture dish 25, is fixedly connected with the side wall of the metal culture dish 25 through a connecting rod 24, namely, when the nut 14 moves, the metal culture dish 25 is synchronously controlled to move along the bottoms of a flushing fluid box 16, a complete culture medium liquid box I17, a cylinder 22, a complete culture medium liquid box II 18 and the like.

In one example of the present utility model, referring to fig. 5, when the culture dish 26 is washed through the bottom of the washing nozzle 19, the washing liquid entering the culture dish 26 needs to be discharged in time, so that a drain hole with a T-shaped vertical section is formed in the bottom wall of the culture dish 26, a sealing block 41 with a T-shaped vertical section is inserted into the drain hole, when the sealing block 41 is completely inserted into the drain hole, the bottom end of the sealing block 41 extends to the outside of the bottom of the metal culture dish 25, when the sealing block 41 is in a free state, the sealing block 41 is inserted into the drain hole in a sealing manner, when the culture dish 26 moves towards the bottom of the washing nozzle 19, a set of transverse sliding rails 29 are arranged in the reagent box 10 under the washing nozzle 19, the medium output dropper 20 and the grinding plate 21, one end of the sliding rail 29 away from the culture dish 26 extends to the inside of the incubation incubator 23, simultaneously, a sliding groove 27 is formed in the bottom of the metal culture dish 25 corresponding to the sliding rail 29, when the culture dish groove 26 moves towards the sliding rail 29, the sliding groove 27 is in sliding connection with the sliding rail 29, a jacking column 30 with the top being in an arc-shaped structure is elastically connected to the sliding rail 29 under the flushing nozzle 19, when the sliding groove 27 slides along the sliding rail 29, the jacking column 30 is compressed until the jacking column 30 moves to the bottom end of the sealing block 41, at the moment, the jacking column 30 stretches to jack the sealing block 41, at the moment, flushing fluid entering the culture dish groove 26 flows out along a gap between the sealing block 41 and the drainage hole, so that the flushing fluid inside the culture dish groove 26 is automatically discharged, and a group of flushing fluid collecting boxes are arranged inside the kit body 10 under the sliding rail 29 and used for collecting waste liquid released in the drainage hole;

specifically, for keeping the stability of automatic connection between sealing block 41 and the wash port, all be provided with one deck magnet piece between sealing block 41 and the inside upper surface of wash port, utilize the magnetism between the magnet piece to inhale the effect, then cooperate the gravity of sealing block 41, the inseparable laminating between automatic sealing block 41 and the wash port is connected, prevents the leakage of culture dish groove 26 inside liquid, and the parcel has the one deck sealing washer on the circumference lateral wall that sealing block 41 and wash port contacted simultaneously for increase waterproof sufficiency.

As a preferred embodiment of the present utility model, referring to fig. 6-7, an incubator 23 is provided with an opening towards one side of a metal culture dish 25, and an elastic rubber baffle 37 is vertically and swingably provided in the opening, the elastic rubber baffle 37 is provided to keep the tightness of the inside of the incubator 23 to a certain extent while the temperature inside the incubator 23 is not affected by the arrangement of the elastic rubber baffle 37, the bottom of a complete culture medium tank ii 18 corresponding to the middle part of the incubator 23 is connected with a culture medium output dropper 20, the culture medium in the complete culture medium tank ii 18 is input into the culture dish 26 placed inside the incubator 23 through the culture medium output dropper 20, a temperature adjusting device (not shown in the drawing) and a carbon dioxide gas conveying device (not shown in the drawing) are detachably connected to one side of the incubator 23, the temperature adjusting device adjusts the inside temperature inside the incubator 23 to 37 °, and controls the carbon dioxide content inside the incubator 23 to 5%, then the pulp tissue is placed inside the incubator 26, a tissue culture medium is subjected to grinding block under the environment of 37 ° and 5% carbon dioxide content, the inside the incubator is subjected to a constant-flow medium is not shown in the inside the incubator 26, and the culture medium is periodically discharged outside the incubator 26, and the culture medium is regularly discharged outside the incubator 23, and the culture medium is not shown in the culture medium is discharged to the culture medium is completely;

specifically, the liquid draining mechanism comprises a liquid draining pipe 32 arranged at one side in the incubation incubator 23, the liquid draining pipe 32 is externally communicated with a liquid draining pipe 35, the bottom of the liquid draining pipe 35 is communicated with a waste liquid storage bin 36 arranged in the reagent box body 10, a pump body 33 is arranged on the liquid draining pipe 32, meanwhile, one side of the liquid draining pipe 32 is connected with an electric lifting rod 34 for controlling the liquid draining pipe 32 to lift, and under the cooperation of the electric lifting rod 34 and the pump body 33, the liquid draining pipe 32 is controlled to output the culture medium in the culture dish groove 26 outwards;

because the nut 14 needs to control the metal culture dish 25 to transfer the metal culture dish 25 into the incubation incubator 23, at the moment, collision can be generated between the connecting rod and the side wall of the incubation incubator 23 to prevent the entrance of the culture dish groove 26, so that the side, facing the connecting rod, of the incubation incubator 23 is provided with the connecting rod slide way 38, the inside of the connecting rod slide way 38 is provided with the split sealing check ring 39, the connecting rod slide way 38 is automatically closed by utilizing the split sealing check ring 39, and meanwhile, the entrance of the connecting rod is not influenced.

In the above embodiment of the utility model, a kit for rapidly separating and extracting dental pulp stem cells is provided, in use, dental pulp is input into a culture dish tank 26 on the upper side of a metal culture dish 25 along a dental pulp input port 12, then a servo motor 13 is started to drive a screw 15 to rotate, a nut 14 is driven to control the movement of the metal culture dish 25, when the culture dish tank 26 moves below a flushing nozzle 19, the dental pulp in the culture dish tank 26 is flushed through the flushing nozzle 19, at the moment, a sealing block 41 is in an opened state under the jack of the jack post 30, flushing fluid entering the culture dish tank 26 flows out along a gap between the sealing block 41 and a drain hole, after flushing is completed, the culture dish tank 26 continues to move to the lower side of a complete culture medium tank I17, a culture medium is injected into the culture dish tank 26 through a culture medium output dropper 20, then the culture tank 26 is controlled to move to the lower side of a grinding plate 21 again, a cylinder 22 is started to control the lifting of the grinding plate 21, the dental pulp is crushed, then the dental pulp is controlled to be transferred into a tissue culture tank 23 in the culture dish 26, the culture tank is stopped at the inner side of the culture dish 26, the inner wall of the culture tank is stopped at 37 ℃ and the inner wall of the culture tank is completely, and the culture medium is removed from the culture tank is periodically and is removed from the inner wall of the culture tank by a tissue culture tank is filled with a tissue culture medium tank under the condition of a certain volume of a medium, and the culture medium is completely is removed from the culture medium tank is filled in a culture medium tank is discharged under the condition of a medium tank is at 37, and then a certain condition is filled into a medium is filled into a culture medium tank is filled in a culture medium tank is at a buffer tank is at the inner container is at the inside a buffer tank is at the inside a container is under the tissue tank is under the tissue.

The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the utility model.

Claims

1. A kit for rapidly isolating and extracting dental pulp stem cells, comprising: a reagent box body (10), a control panel (11) and an dental pulp input port (12); the dental pulp input port (12) is arranged on one side of the top of the reagent box body (10), a flushing fluid tank (16), a complete culture medium liquid tank I (17), a cylinder (22) and a complete culture medium liquid tank II (18) are arranged on the top of the reagent box body (10) in the same linear direction of the dental pulp input port (12) at intervals, a metal culture dish (25) is circularly moved in the direction of the flushing fluid tank (16), the complete culture medium liquid tank I (17), the cylinder (22) and the complete culture medium liquid tank II (18) in the reagent box body (10), a culture dish groove (26) with a circular structure is formed in the top of the metal culture dish (25), a power mechanism for driving the metal culture dish (25) to move along the inner length direction of the reagent box body (10) is connected to one side of the metal culture dish (25), a flushing spray nozzle (19) is connected to the bottom of the flushing fluid tank (16), a culture medium liquid output (20) is connected to the bottom of the complete culture medium liquid tank I (17), a grinding plate (21) is connected to the bottom of the cylinder (22), and the culture medium liquid tank II (23) is communicated with the bottom of the complete culture medium liquid tank II.

2. The kit capable of rapidly separating and extracting dental pulp stem cells according to claim 1, wherein a control panel (11) is arranged on one side of the top of the kit body (10), and solution inlets are communicated upwards at the tops of a flushing liquid tank (16), a complete medium liquid tank I (17) and a complete medium liquid tank II (18).

3. The kit capable of rapidly separating and extracting dental pulp stem cells according to claim 2, wherein the power mechanism comprises a screw rod (15) rotatably arranged on one side of the interior of the kit body (10), one end of the screw rod (15) is connected with a servo motor (13), the other end of the screw rod is rotatably connected to the inner wall of the kit body (10) through a bearing, a nut (14) is connected to the screw rod (15) in a threaded manner, a guiding device for limiting the rotation of the screw rod is arranged on the nut (14), the servo motor (13) is electrically connected with the control panel (11), and one side of the nut (14) facing the metal culture dish (25) is fixedly connected with the side wall of the metal culture dish (25) through a connecting rod (24).

4. The kit capable of rapidly separating and extracting dental pulp stem cells according to claim 3, wherein a drain hole with a T-shaped structure is formed in the inner bottom wall of the culture dish groove (26), a sealing block (41) with a T-shaped structure is inserted into the drain hole, when the sealing block (41) is completely inserted into the drain hole, the bottom end of the sealing block (41) extends to the bottom outside of the metal culture dish (25), when the sealing block (41) is in a free state, the sealing block (41) is in sealing insertion with the drain hole, a group of transverse sliding rails (29) are arranged in the kit body (10) right below the flushing nozzle (19), the medium liquid output dropper (20) and the grinding plate (21), one end, far away from the culture dish groove (26), of the sliding rail (29) extends to the inside of the incubation culture dish (23), a sliding groove (27) is formed in the bottom of the metal culture dish (25) corresponding to the sliding rail (29), the sliding groove (27) is in sliding connection with the sliding rail (29), and the top end of the sliding rail (29) right below the flushing nozzle (19) is in elastic connection with an arc-shaped column (30).

5. The kit for rapidly separating and extracting dental pulp stem cells according to claim 4, wherein a layer of magnet sheet is arranged between the sealing block (41) and the upper surface inside the drain hole, and a layer of sealing ring is wrapped on the circumferential side wall of the sealing block (41) contacted with the drain hole.

6. The kit capable of rapidly separating and extracting dental pulp stem cells according to claim 5, wherein one side of the incubation incubator (23) facing the metal culture dish (25) is provided with an opening, an elastic rubber baffle (37) is vertically arranged in the opening in a swinging manner, the bottom of a complete culture medium liquid box II (18) corresponding to the middle part in the incubation incubator (23) is connected with a culture medium liquid output dropper (20), one side of the incubation incubator (23) is detachably connected with a temperature regulating device and a carbon dioxide gas conveying device, and one side of the inside of the incubation incubator (23) is provided with a liquid draining mechanism.

7. The kit for rapidly separating and extracting dental pulp stem cells according to claim 6, wherein the liquid draining mechanism comprises a liquid draining pipe (32) arranged on one side of the interior of the incubation incubator (23), the liquid draining pipe (32) is externally communicated with a liquid draining pipe (35), the bottom of the liquid draining pipe (35) is communicated with a waste liquid storage bin (36) arranged in the interior of the kit body (10), the liquid draining pipe (32) is provided with a pump body (33), and one side of the liquid draining pipe (32) is connected with an electric lifting rod (34) for controlling the lifting of the liquid draining pipe (32).

8. Kit for rapid isolation and extraction of dental pulp stem cells according to claim 7, wherein a connecting rod slide (38) is provided on one side of the incubation incubator (23) facing the connecting rod, and a split sealing retainer ring (39) is provided inside the connecting rod slide (38).