CN113881565B - Mesenchymal stem cell culture and separation device - Google Patents

Abstract

The invention discloses a mesenchymal stem cell culture separation device, which belongs to the field of cell culture and comprises an incubator, wherein a liquid draining box is arranged on the lower side of the incubator, a support is fixed on the outer wall of the liquid draining box, a sliding groove is formed in the top of the support, a sliding rod is arranged in the sliding groove, the outer wall of the incubator is slidably arranged with the sliding rod through a transverse plate, a spring is wound on the upper part of the sliding rod, a liquid adding pipe is arranged on the top of the incubator, a cross partition plate is arranged in the incubator, a closing plate is arranged at the bottom of the incubator, and the outer side of the closing plate is slidably arranged with the inner wall of the liquid draining box. The invention can increase the adsorption area in the incubator during cell culture, is convenient for a large amount of cell culture, can separate high-activity and low-activity cells in culture, can avoid accidental separation of the high-activity cells in separation, reduces loss, and can conveniently and rapidly take out and collect the cells from the incubator after the culture is finished, thereby being convenient and quick to operate.

Description

A device for culturing and separating mesenchymal stem cells

technical field

The invention relates to the technical field of cell culture, in particular to a mesenchymal stem cell culture and separation device.

Background technique

Mesenchymal stem cells are a kind of pluripotent stem cells, which have all the common characteristics of stem cells, that is, self-renewal and multidirectional differentiation capabilities. It is also the most clinically used, and combined with hematopoietic stem cells can improve the success rate of transplantation and accelerate hematopoietic reconstruction.

After retrieval, Chinese Patent No. CN 212476783 U discloses a mesenchymal stem cell culture and separation device, including a culture bottle and a bottle cap arranged on the culture bottle. The culture bottle is a cylindrical bottle structure, and the position of the bottle mouth is A bottle cap is fixedly installed at the upper end of the culture bottle, and a liquid inlet and a liquid outlet are arranged on both sides of the upper end of the culture bottle. Liquid outlet bottle cap.

The culture and separation device used in the prior art still has the following disadvantages in actual use: when the separation device mixes the culture solution, it is easy to cause the cells adsorbed on the inner wall of the culture bottle to be accidentally separated, resulting in low cell culture efficiency. The invention proposes a device for culturing and separating mesenchymal stem cells.

Contents of the invention

The purpose of the present invention is to solve the defects in the prior art, and propose a mesenchymal stem cell culture and separation device.

In order to achieve the above object, the present invention adopts the following technical solutions:

A mesenchymal stem cell culture and separation device, comprising an incubator, the underside of the incubator is provided with a drainage box, the outer wall of the drainage box is fixed with a bracket, the top of the bracket is provided with a chute, the A slide bar is installed in the chute, and the outer wall of the incubator is slidably installed through a horizontal plate and the slide bar. A spring is wound around the upper part of the slide bar, and a liquid feeding pipe is installed on the top of the incubator. A cross partition is installed inside, the bottom of the incubator is provided with a closing plate, the outer side of the closing plate is slidably installed with the inner wall of the drain tank, the lower side of the closing plate is provided with a jacking mechanism, and the incubator The lower side of the incubator is provided with an opening and closing mechanism installed on the horizontal plate, and the bottom of the incubator is detachably connected with the closing plate through the opening and closing mechanism.

Further, the jacking mechanism includes a two-way screw rod, the two ends of the two-way screw rod are rotatably installed with the discharge tank, the two-way screw rod is driven by a motor, and two moving seats are mounted on symmetrical threads on the two-way screw rod , the top ends of the moving seats are hingedly mounted with support arms, and the top ends of the support arms are hingedly mounted with the bottom wall of the closing plate;

The opening and closing mechanism includes a rotating rod that is rotatably installed with the horizontal plate. The top end of the rotating rod extends to the upper side of the horizontal plate and a knob is installed. The bottom end of the rotating rod is equipped with a limit piece, and the limit piece The top surface is in sliding contact with the bottom wall of the closure plate.

Further, a slider is fixedly installed on the outer side of the closure plate, a slide rail is fixedly installed on the inner wall of the liquid discharge box, and the slider is slidably arranged in the slide rail.

Further, a sealing ring is fixed on the top surface of the closing plate, and the sealing ring is in sliding contact with the outer wall at the bottom of the incubator.

Further, two horizontal plates are fixed on the outer wall of the incubator, and the horizontal plates are both slidably installed with the slide bar, and the spring is located above the upper horizontal plate.

Another object of the present invention is: take out in the convenient incubator of cell after cell culture, therefore the present invention proposes following technical scheme simultaneously on the basis of above-mentioned technical scheme:

Further, the inside of the incubator is rotatably installed with a one-way screw rod, the top of the one-way screw rod extends to the upper side of the incubator and is equipped with a knob, and the thread sleeve on the one-way screw rod is provided with a threaded tube , the middle part of the cross partition is provided with a vertical hole, the one-way screw rod is located in the vertical hole, and the outer wall of the one-way screw rod is fixed with four fan-shaped frames, and each fan-shaped frame is respectively Frictional contact with the inner wall of the incubator and the side wall of the cross partition.

Further, there is a gap between the top of the cross partition and the inner top wall of the incubator.

Further, a positioning rod is installed on the top wall of the closing plate, and the positioning rod is slidably inserted inside the casing of the incubator.

Compared with the prior art, the beneficial effects of the present invention are:

1. The present invention arranges a detachable closing plate on the bottom wall of the incubator, and uses a jacking mechanism to repeatedly lift the closing plate and the incubator, thereby shaking the culture solution in the incubator, and utilizing the shaking of the culture solution Flush the cells inside the culture flask, separate the highly active cells from the low active cells, and control the closing plate to separate from the incubator through the lifting mechanism after separation, and discharge the discarded culture medium and its low active cells Incubator for cell separation operation.

2. The interior of the incubator in the present invention is equipped with a cross partition, which can increase the adsorption area of cells in the incubator and utilize the mass cultivation of cells.

3. The present invention sets the fan-shaped frame and moves the fan-shaped frame up and down through the mutual cooperation of the one-way screw rod and the threaded pipe, and uses the moving fan-shaped frame to rub the inner wall of the incubator and the side wall of the cross partition, so that the adsorption The cell separation facilitates the collection of cells.

In summary, the present invention can increase the adsorption area inside the incubator during cell culture, facilitate the cultivation of a large number of cells, separate highly active cells from low active cells in culture, and avoid high active cell accidents during separation. Separation reduces loss, and it is also convenient and quick to take out and collect cells from the incubator after the culture is over, and the operation is convenient.

Description of drawings

The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the description, and are used together with the embodiments of the present invention to explain the present invention, and do not constitute a limitation to the present invention.

FIG. 1 is a schematic diagram of the overall structure of Embodiment 1 of the present invention;

Figure 2 is an internal sectional view of Figure 1;

Fig. 3 is the schematic diagram of the sliding installation of the incubator through the horizontal plate and the support in the present invention;

Fig. 4 is the schematic diagram when the incubator is pushed up by the closed plate in the present invention;

Fig. 5 is a schematic diagram when the closing plate is separated from the incubator downwards in the present invention;

Figure 6 is a schematic diagram of the installation of the bottom of the closing plate and the jacking mechanism in the present invention;

7 is a schematic diagram of the overall structure of Embodiment 2 of the present invention;

Figure 8 is an internal sectional view of Figure 7;

FIG. 9 is a schematic diagram of the fan-shaped frame in FIG. 7 moving downward.

In the figure: 1 drain tank, 2 incubator, 3 bracket, 4 slide rod, 5 horizontal plate, 6 spring, 7 liquid filling pipe, 8 motor, 9 cross partition, 10 closing plate, 11 slide block, 12 slide rail , 13 sealing ring, 14 two-way screw, 15 moving seat, 16 arm, 17 rotating rod, 18 limit plate, 19 positioning rod, 20 one-way screw, 21 threaded pipe, 22 sector frame.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them.

In describing the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", " The orientation or positional relationship indicated by "outside", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, so as to Specific orientation configurations and operations, therefore, are not to be construed as limitations on the invention.

Embodiment one

Referring to Figures 1-6, a mesenchymal stem cell culture and separation device includes an incubator 2, the underside of the incubator 2 is provided with a drainage box 1, the outer wall of the drainage box 1 is fixed with a support 3, and the top of the support 3 is provided with a There is a chute, a slide bar 4 is installed in the chute, the outer wall of the incubator 2 slides through the horizontal plate 5 and the slide bar 4, and the upper part of the slide bar 4 is wound with a spring 6, as shown in Figure 3, in the incubator The outer wall of 2 is fixed with two horizontal plates 5, and the horizontal plates 5 are all slidably installed with the slide bar 4, and the spring 6 is positioned above the horizontal plate 5 on the upper side.

The top of the incubator 2 is equipped with a liquid feeding pipe 7, and a cross partition 9 is installed in the incubator 2, leaving a gap between the top of the cross partition 9 and the inner top wall of the incubator 2. It is convenient for the culture solution to be quickly and evenly distributed in the interior of the incubator 2 .

The culture solution containing cells is added to the interior of the incubator 2 through the liquid feeding pipe 7, and the cells are divided in the interior of the incubator 2.

The bottom of the incubator 2 is provided with a closing plate 10, the top surface of the closing plate 10 is fixed with a sealing ring 13, and the sealing ring 13 is in sliding contact with the outer wall at the bottom of the incubating box 2.

The sealing ring 13 increases the sealing between the bottom of the incubator 2 and the top wall of the closing plate 10, and the diameter of the closing plate 10 is greater than the outer diameter of the bottom of the incubator 2 to ensure that the bottom of the incubator 2 can be closed.

A positioning rod 19 is installed on the top wall of the closing plate 10 , and the positioning rod 19 is slidably inserted in the housing of the incubator 2 . The outer side of the closing plate 10 is slidably installed with the inner wall of the drainage box 1, the outer side of the closing plate 10 is fixedly installed with a slider 11, and the inner wall of the drainage box 1 is fixedly installed with a slide rail 12, and the slider 11 is slidably arranged in the sliding rail 12 .

The lower side of the closing plate 10 is provided with a jacking mechanism, the lower side of the incubator 2 is provided with an opening and closing mechanism installed with the horizontal plate 5, and the bottom of the incubator 2 is detachably connected with the closing plate 10 through the opening and closing mechanism.

The jacking mechanism includes a two-way screw rod 14, the two ends of the two-way screw rod 14 are installed in rotation with the discharge tank 1, the two-way screw rod 14 is driven by a motor 8, and two movable seats 15 are installed on the symmetrical thread on the two-way screw rod 14, and the movable seat The tops of 15 are all hingedly equipped with support arms 16, and the tops of support arms 16 are all hingedly installed with the bottom wall of the closing plate 10;

Opening and closing mechanism comprises the rotating rod 17 that is installed with horizontal plate 5 rotations, and the top end of rotating rod 17 extends to the upper side of horizontal plate 5 and is equipped with knob, and the bottom end of rotating rod 17 is equipped with limit piece 18, and the limit plate 18 The top surface is in sliding contact with the bottom wall of the closure plate 10 .

Rotate the rotating rod 17 to rotate the stop plate 18 to the bottom wall of the closing plate 10, at this time, the closing plate 10 can be restricted from separating from the bottom of the incubator 2.

Then drive the two-way screw mandrel 14 to rotate in the forward direction by the motor 8, and the two-way screw mandrel 14 simultaneously controls the two moving seats 15 to approach each other. 8. When the two-way screw rod 14 is reversely driven to rotate, the two moving seats 15 are controlled to move away from each other, thereby controlling the closing plate 10 and the upper incubator 2 to move down, and repeatedly moving the closing plate 10 and the incubator 2 up and down, and then the cultivation The nutrient solution inside the case 2 is shaken, and the swaying nutrient solution can wash the inwall of the incubator 2 and the side wall of the cross partition 9, wherein the cells with lower activity and death can be separated from the inwall of the incubator 2 and the cross partition. The side wall of the plate 9 is separated, and the highly active cells continue to be adsorbed on the inner wall of the incubator 2 and the side wall of the cross partition 9 .

The above process is more efficient than stirring, and avoids the accidental separation of highly active cells.

After the high-activity cells are separated from the low-activity cells, the rotating shaft 17 is reversed, the limit of the stop plate 18 to the closing plate 10 is canceled, and then the closing plate 10 is separated from the incubator 2 by the jacking mechanism. At this time, the discarded culture medium And the low-activity cells and dead cells mixed therein can be discharged into the drain box 1, and finally discharged from the device.

The horizontal plate 5 arranged on the outside of the incubator 2 can increase the stability when the incubator 2 moves up and down, and the spring 6 arranged on the slide bar 4 can increase the stability of the seal when the closing plate 10 and the incubator 2 move up and down sex.

Embodiment two

Referring to Figures 7-9, the second embodiment is based on the first embodiment above, and a one-way screw 20 is rotated inside the incubator 2, and the top of the one-way screw 20 extends to the upper side of the incubator 2 and Knobs are installed, the threaded sleeve on the one-way screw mandrel 20 is provided with a threaded pipe 21, the middle part of the cross partition 9 is penetrated with a vertical hole, the one-way screw mandrel 20 is located in the vertical hole, and the outer wall of the one-way screw mandrel 20 is fixed. There are four fan-shaped frames 22, and each fan-shaped frame 22 is in frictional contact with the inner wall of the incubator 2 and the side wall of the cross partition 9 respectively. The fan-shaped frame 22 is fan-shaped, and the upper surface and the lower surface are hollow.

After the highly active cells are separated from the low active cells and dead cells, by turning the one-way screw 20, the threaded tube 21 and the fan-shaped frame 22 installed with it can be controlled to move up and down, so that the highly active cells can be cultured by using the fan-shaped frame 22. Separate on the inner wall of case 2 and the side wall of cross dividing plate 9, conveniently it is collected. Operation is faster and more efficient.

The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto, any person familiar with the technical field within the technical scope disclosed in the present invention, according to the technical solution of the present invention Any equivalent replacement or change of the inventive concepts thereof shall fall within the protection scope of the present invention.

Claims (6)

1. A device for culturing and separating mesenchymal stem cells, comprising an incubator (2), characterized in that a drain tank (1) is provided on the underside of the incubator (2), and the drain tank (1) The outer wall of the incubator (3) is fixed with a bracket (3), the top of the bracket (3) is provided with a chute, the slide bar (4) is installed in the chute, and the outer wall of the incubator (2) passes through the horizontal plate (5) Slidingly installed with the slide bar (4), the upper part of the slide bar (4) is wound with a spring (6), the top of the incubator (2) is equipped with a liquid feeding pipe (7), and the incubator (2) ) is installed with a cross partition (9), the bottom of the incubator (2) is provided with a closing plate (10), and the outer side of the closing plate (10) is slidingly installed with the inner wall of the drain tank (1), so The lower side of the closed plate (10) is provided with a jacking mechanism, the lower side of the incubator (2) is provided with an opening and closing mechanism installed with the horizontal plate (5), and the bottom of the incubator (2) is opened The closing mechanism is detachably connected with the closing plate (10); The jacking mechanism includes a two-way screw rod (14), the two ends of the two-way screw rod (14) are rotatably installed with the discharge tank (1), and the two-way screw rod (14) is driven by a motor (8). The two-way screw rod (14) is symmetrically threaded with two moving seats (15), and the tops of the moving seats (15) are hingedly equipped with support arms (16), and the top ends of the support arms (16) are connected to The bottom wall of the closing plate (10) is hingedly installed; The opening and closing mechanism includes a rotating rod (17) that is rotatably installed with the horizontal plate (5). The top end of the rotating rod (17) extends to the upper side of the horizontal plate (5) and is equipped with a knob. The rotating rod ( The bottom end of 17) is equipped with a limiting piece (18), and the top surface of the limiting piece (18) is in sliding contact with the bottom wall of the closing plate (10); The inside of the incubator (2) is rotatably installed with a one-way screw rod (20). The top of the one-way screw rod (20) extends to the upper side of the incubator (2) and is equipped with a knob. The one-way screw rod (20) A threaded pipe (21) is provided on the screw rod (20), and a vertical hole is formed in the middle of the cross partition (9), and the one-way screw rod (20) is located in the vertical hole. Four fan-shaped frames (22) are fixed on the outer wall of the one-way screw rod (20), and each of the fan-shaped frames (22) is respectively in frictional contact with the inner wall of the incubator (2) and the side wall of the cross partition (9) . 2. A mesenchymal stem cell culture and separation device according to claim 1, characterized in that a slider (11) is fixedly installed on the outer side of the closing plate (10), and the drain box (1) A slide rail (12) is fixedly installed on the inner wall, and the slide block (11) is slidably arranged in the slide rail (12). 3. A mesenchymal stem cell culture and separation device according to claim 1, characterized in that a sealing ring (13) is fixed on the top surface of the closing plate (10), and the sealing ring (13) is in contact with the culture The outer wall of the bottom end of the box (2) is in sliding contact. 4. A mesenchymal stem cell culture and separation device according to claim 1, characterized in that two horizontal plates (5) are fixed on the outer wall of the incubator (2), and the horizontal plates (5) are both Slidingly installed with the slide bar (4), the spring (6) is located above the upper horizontal plate (5). 5. The device for culturing and separating mesenchymal stem cells according to claim 1, characterized in that there is a gap between the top of the cross partition (9) and the inner top wall of the incubator (2). 6. A device for culturing and separating mesenchymal stem cells according to claim 1, characterized in that a positioning rod (19) is installed on the top wall of the closing plate (10), and the positioning rod (19) is slidably inserted Inside the shell of the incubator (2).