CN106967584A - A kind of automated cell suspension blows and beats evenly mixing device - Google Patents
A kind of automated cell suspension blows and beats evenly mixing device Download PDFInfo
- Publication number
- CN106967584A CN106967584A CN201710307386.4A CN201710307386A CN106967584A CN 106967584 A CN106967584 A CN 106967584A CN 201710307386 A CN201710307386 A CN 201710307386A CN 106967584 A CN106967584 A CN 106967584A
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- China
- Prior art keywords
- liquid
- arm
- transfering gun
- motor
- rotating disk
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- 239000006285 cell suspension Substances 0.000 title claims abstract description 21
- 238000009434 installation Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 description 7
- 230000033001 locomotion Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Evenly mixing device is blown and beaten the invention discloses a kind of automated cell suspension, mainly including support, liquid-transfering gun and culture dish；The support installs frame straight up, frame lower is inserted in movable sleeving, movable sleeving is provided with fixed knob, a transverse arm is fixedly connected with movable sleeving, transverse arm and motor rotating disk are connected by rotating shaft I, culture dish can be installed in the rotation with surface parallel to motor rotating disk, culture dish seat top under the driving of motor loaded on the culture dish seat on motor rotating disk；Rotating shaft II is installed in upper rack, rotating shaft II connects the liquid-transfering gun arm of force of level, liquid-transfering gun one end away from rotating shaft II on the liquid-transfering gun arm of force by chuck, the pressing button on liquid-transfering gun top is mounted in the movable end of switch arm, the fixing end of switch arm is arranged on the liquid-transfering gun arm of force, and switch arm is driven by motor.The present invention can easily control the speed that cell suspension is blown and beaten, and be efficiently completed the mixed uniformly task of cell suspension, save the time of experimenter.
Description
Technical field
The present invention relates to field of bioexperiment technology, and in particular to a kind of automated cell suspension blows and beats evenly mixing device.
Background technology
Cell field is goed deep into the development that deepens continuously studied with bioscience, increasing research, is related to cell
The microstructures such as form, albumen, DNA molecules, therefore, cell culture technology are widely used in Bioexperiment and as basis
, it is necessary to which artificial blow and beat cell suspension of experimenter mixes under one of condition, existence conditions, cell is set to be uniformly dispersed, as list
Cell suspension, and it is appropriate to blow and beat power control, it is too fast it is too quickly can make cellular damage, and the excessively slow scattered inequality of too slow then cell, easily
Unite, so as to influence the growth of cell.Particularly when handling high-volume cell, experimenter's plenty of time is occupied.Therefore, send out
A kind of bright automatic piping and druming evenly mixing device,
It is efficient to be well mixed cell suspension, and save the time of experimenter.
The content of the invention
The present invention blows and beats evenly mixing device there is provided a kind of automated cell suspension in order to overcome the above-mentioned deficiencies of the prior art,
It is efficient to be well mixed cell suspension, and save the time of experimenter.
The technical scheme is that, a kind of automated cell suspension blows and beats evenly mixing device, mainly including support, liquid-transfering gun and
Culture dish；It is characterized in that：The support installs frame straight up, and frame lower is inserted in movable sleeving, and movable sleeving is provided with
For fixed knob movable sleeving being fixed in frame, be fixedly connected with a transverse arm, transverse arm and motor rotating disk pass through on movable sleeving
Rotating shaft I is connected, and motor rotating disk I can be rotated up carrying out being provided with motor in position adjustments, motor rotating disk around the shaft, turn loaded on motor
Culture dish seat on disk can install training under the driving of motor in the rotation with surface parallel to motor rotating disk, culture dish seat top
Support ware；Rotating shaft II is installed, rotating shaft II connects the liquid-transfering gun arm of force of level in upper rack, the liquid-transfering gun arm of force can around the shaft II
Certain amplitude swings back and forth in horizontal direction, liquid-transfering gun one end away from rotating shaft II on the liquid-transfering gun arm of force by chuck, moves
The pressing button on liquid rifle top is mounted in the movable end of switch arm, and the fixing end of switch arm is arranged on the liquid-transfering gun arm of force, switch arm
Driven by motor.
Further, above-mentioned automated cell suspension piping and druming evenly mixing device, described switch arm, rotating shaft II, motor rotating disk
Provided with different motors, and these motors are controlled by the power switch on support.
Further, above-mentioned automated cell suspension piping and druming evenly mixing device, described motor rotating disk can be around the shaft I vertical
Rotation 0-90 degree in plane.
Further, above-mentioned automated cell suspension piping and druming evenly mixing device, described frame is at liquid-transfering gun arm of force swing
Provided with arc-shaped guide rail.
Before operation, liquid-transfering gun is installed, and the position of culture dish is adjusted by movable sleeving, rotating shaft I, liquid level is soaked
Moisten liquid-transfering gun pipette tips, culture dish seat produces an inclined angle, so that the liquid in culture dish is built up to side；Electricity is opened again
Source switch, culture dish seat drive culture dish rotation, the liquid-transfering gun arm of force at the uniform velocity back and forth movement, liquid relief in the arc-shaped guide rail in frame
Up and down motion of the button by switch arm control law is pressed at the top of rifle, so that constant piping and druming；Liquid-transfering gun pipette tips use disposable rifle
Head, it is replaceable, it is to avoid cross pollution.
The beneficial effects of the invention are as follows：The device can easily control the speed that cell suspension is blown and beaten, and be efficiently completed
The mixed uniformly task of cell suspension, saves the time of experimenter, experiment success rate and efficiency is ensure that well.
Brief description of the drawings
Fig. 1 is that a kind of cell suspension blows and beats evenly mixing device front view.
Fig. 2 is Fig. 1 partial enlarged drawing.
Fig. 3 is partial top views of the Fig. 1 at the liquid-transfering gun arm of force.
In figure：1. support, 2. power switches, 3. motor rotating disks, 4, culture dish seat, 5. fixed knobs, 6. movable sleevings, 7.
Frame, the 8. liquid-transfering gun arm of forces, 9. switch arms, 10. chucks, 11. liquid-transfering guns, 12 culture dishes, 13. transverse arms, 14, rotating shafts I, 15.
Rotating shaft II, 16. arc-shaped guide rails.
Embodiment
The present invention is further described below in conjunction with the accompanying drawings.
As Figure 1-3, a kind of automated cell suspension piping and druming evenly mixing device, mainly includes support 1, liquid-transfering gun 11 and culture
Ware 12；The support 1 installs frame 7 straight up, and movable sleeving 6 is inserted in the bottom of frame 7, and movable sleeving 6, which is provided with, to be used to move
Dynamic set 6 is fixed on the fixed knob 5 in frame 7, movable sleeving 6 and is fixedly connected with a transverse arm 13, and transverse arm 12 and motor rotating disk 3 pass through
The connection of rotating shaft I 14, motor rotating disk 3 I 14 can be rotated up carrying out being provided with motor in position adjustments, motor rotating disk 3 around the shaft, be loaded on
Culture dish seat 4 on motor rotating disk 3 can be in the rotation with surface parallel to motor rotating disk 3, culture dish seat 4 under the driving of motor
Top is installed by culture dish 12；Rotating shaft II 15 is installed, rotating shaft II 15 connects the liquid-transfering gun arm of force 8 of level, moves in the top of frame 7
The liquid rifle arm of force 8 can around the shaft II 15 in the horizontal direction certain amplitude swing back and forth, liquid-transfering gun 11 by chuck 10 be mounted in liquid relief
One end away from rotating shaft II 15 on the rifle arm of force 8, the pressing button on the top of liquid-transfering gun 11 is mounted in the movable end of switch arm 9, switch arm 9
Fixing end be arranged on the liquid-transfering gun arm of force 8 on, switch arm 9 is driven by motor.Described switch arm 9, rotating shaft II 15, motor rotating disk
3 are provided with different motors, and these motors are controlled by the power switch 2 on support 1.Described motor rotating disk 3 can be around
Rotating shaft I 14 rotates 0-90 degree in perpendicular.Described frame 7 is provided with arc-shaped guide rail 16 at the swing of the liquid-transfering gun arm of force 8.
During using the device, culture dish 12 is placed on culture dish base 4 first, an inclination angle is formed, movable sleeving is adjusted
6, the liquid level of nutrient solution is infiltrated the pipette tips of liquid-transfering gun 11, turn on the power switch 2, switch arm 9 comes and goes pressing liquid-transfering gun 11 automatically,
At the same time, liquid-transfering gun rifle arm 8 also does back and forth movement by a small margin, and culture dish 12 remains a constant speed rotation, realization on motor rotating disk 3
Automatic piping and druming cell suspension, makes its uniform, it is ensured that cytoactive and growth, and liberates experimenter's both hands, can be in this device
Other operations are handled during work, save particularly important during the time, especially tangible mass disposal cell of experimenter.
Claims (4)
1. a kind of automated cell suspension blows and beats evenly mixing device, mainly including support, liquid-transfering gun and culture dish；It is characterized in that：Institute
The frame of support installation straight up is stated, frame lower is inserted in movable sleeving, and movable sleeving, which is provided with, to be used to movable sleeving being fixed on machine
A transverse arm is fixedly connected with fixed knob on frame, movable sleeving, transverse arm and motor rotating disk are connected by rotating shaft I, and motor rotating disk can
I it is rotated up carrying out being provided with motor in position adjustments, motor rotating disk around the shaft, loaded on the culture dish seat on motor rotating disk in motor
Driving under can be in the rotation with surface parallel to motor rotating disk, culture dish is installed in culture dish seat top；Installed in upper rack
There is a rotating shaft II, rotating shaft II connects the liquid-transfering gun arm of force of level, the liquid-transfering gun arm of force can II certain amplitude in the horizontal direction around the shaft
Swing back and forth, liquid-transfering gun one end away from rotating shaft II, the pressing button on liquid-transfering gun top on the liquid-transfering gun arm of force by chuck
Mounted in the movable end of switch arm, the fixing end of switch arm is arranged on the liquid-transfering gun arm of force, and switch arm is driven by motor.
2. cell according to claim 1 blows and beats device, it is characterised in that：Described switch arm, rotating shaft II, motor rotating disk
Provided with different motors, and these motors are controlled by the power switch on support.
3. cell according to claim 1 blows and beats device, it is characterised in that：Described motor rotating disk I can erected around the shaft
Rotation 0-90 degree in straight plane.
4. cell according to claim 1 blows and beats device, it is characterised in that：Described frame is at liquid-transfering gun arm of force swing
Provided with arc-shaped guide rail.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710307386.4A CN106967584A (en) | 2017-05-04 | 2017-05-04 | A kind of automated cell suspension blows and beats evenly mixing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710307386.4A CN106967584A (en) | 2017-05-04 | 2017-05-04 | A kind of automated cell suspension blows and beats evenly mixing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106967584A true CN106967584A (en) | 2017-07-21 |
Family
ID=59331986
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710307386.4A Pending CN106967584A (en) | 2017-05-04 | 2017-05-04 | A kind of automated cell suspension blows and beats evenly mixing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106967584A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108330097A (en) * | 2018-03-16 | 2018-07-27 | 苏杰 | A kind of cell culture, detection method |
| CN110551632A (en) * | 2019-07-25 | 2019-12-10 | 南方医科大学珠江医院 | Real-time monitoring cell culture system and culture method thereof |
| CN111925923A (en) * | 2020-09-17 | 2020-11-13 | 天津速一腾高新技术有限公司 | A extrusion is blown and is beaten device for breaking up cell |
| CN114525206A (en) * | 2021-11-22 | 2022-05-24 | 浙江泰林医学工程有限公司 | Automatic cell preparation device |
| CN114703061A (en) * | 2022-06-07 | 2022-07-05 | 中国人民解放军总医院第六医学中心 | Be used for cell culture with multi-functional intelligent cell culture dish |
| CN117683613A (en) * | 2024-02-04 | 2024-03-12 | 科泰(山东)健康营养有限公司 | Experimental equipment for influence of choline bitartrate on lung cancer cells |
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| US6447728B1 (en) * | 1998-10-16 | 2002-09-10 | Dade Behring Marburg Gmbh | Exchangeable oscillating pipette needle |
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| CN104152343A (en) * | 2014-07-16 | 2014-11-19 | 北京赛智创业科技有限公司 | Spin coating device |
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| CN106367343A (en) * | 2016-08-29 | 2017-02-01 | 宁波键生物科技有限公司 | Full-automatic intelligent cell culture device and control method thereof |
| CN206986152U (en) * | 2017-05-04 | 2018-02-09 | 南昌大学 | A kind of automated cell suspension blows and beats evenly mixing device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| DE2211098A1 (en) * | 1972-03-08 | 1973-09-13 | Guenther Kreft | Suction member for pipettes - sliding over the upper end |
| US6447728B1 (en) * | 1998-10-16 | 2002-09-10 | Dade Behring Marburg Gmbh | Exchangeable oscillating pipette needle |
| JP2002346356A (en) * | 2001-05-25 | 2002-12-03 | Nikuni:Kk | Gas/liquid mixing dissolving device |
| JP2004089849A (en) * | 2002-08-30 | 2004-03-25 | Yasukatsu Inouchi | Suction and discharging apparatus for minute amount of liquid |
| CN201316623Y (en) * | 2008-12-04 | 2009-09-30 | 东北师范大学 | Constant-speed booster for pipettor |
| CN202221940U (en) * | 2011-09-05 | 2012-05-16 | 罗耿荣 | Swing-preventing motor of high-precision electric pipette |
| CN102586091A (en) * | 2012-03-01 | 2012-07-18 | 山东省千佛山医院 | Disposable cell passage, blowing, beating and subpackaging suction tube |
| CN203862266U (en) * | 2014-05-09 | 2014-10-08 | 虞海峰 | Electric liquid relief device for medical examination |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108330097A (en) * | 2018-03-16 | 2018-07-27 | 苏杰 | A kind of cell culture, detection method |
| CN108330097B (en) * | 2018-03-16 | 2020-10-23 | 天信和(苏州)生物科技有限公司 | Cell culture and detection method |
| CN110551632A (en) * | 2019-07-25 | 2019-12-10 | 南方医科大学珠江医院 | Real-time monitoring cell culture system and culture method thereof |
| CN111925923A (en) * | 2020-09-17 | 2020-11-13 | 天津速一腾高新技术有限公司 | A extrusion is blown and is beaten device for breaking up cell |
| CN114525206A (en) * | 2021-11-22 | 2022-05-24 | 浙江泰林医学工程有限公司 | Automatic cell preparation device |
| CN114703061A (en) * | 2022-06-07 | 2022-07-05 | 中国人民解放军总医院第六医学中心 | Be used for cell culture with multi-functional intelligent cell culture dish |
| CN117683613A (en) * | 2024-02-04 | 2024-03-12 | 科泰(山东)健康营养有限公司 | Experimental equipment for influence of choline bitartrate on lung cancer cells |
| CN117683613B (en) * | 2024-02-04 | 2024-04-19 | 科泰(山东)健康营养有限公司 | Experimental equipment for influence of choline bitartrate on lung cancer cells |
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| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170721 |
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| WD01 | Invention patent application deemed withdrawn after publication |