CN205528795U - Device of desorption cumulus cell - Google Patents
Device of desorption cumulus cell Download PDFInfo
- Publication number
- CN205528795U CN205528795U CN201620347067.7U CN201620347067U CN205528795U CN 205528795 U CN205528795 U CN 205528795U CN 201620347067 U CN201620347067 U CN 201620347067U CN 205528795 U CN205528795 U CN 205528795U
- Authority
- CN
- China
- Prior art keywords
- culture dish
- layer
- sidewall
- druming
- piping
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Device of desorption cumulus cell belongs to the biotechnology field. The problem of current device of desorption cumulus cell cell in the culture dish in operation process receive the environmental impact big, it is poor for the piping and druming needle provides the pressure signal accuracy easily to influence cell activity and adopt the gasbag is solved. The utility model discloses a culture dish installation device is cylindrical structures, and partly lateral wall is fixed with cylindrical structures's bottom surface, and another part lateral wall is divided equally with cylindrical structures top disc and bottom disc and is left, cylindrical structures's top is equipped with the circular transparent sheet metal of three -layer, and the chamber is placed to culture dish installation device's bottom surface, the transparent sheet metal of first layer and the partial lateral wall constitution culture dish of fixing on the bottom surface, and the pressure signal output port of force pump passes through the air duct and communicates with piping and druming needle intake port, the one end and the inlet end of piping and druming needle of air duct are all fixed in operating handle. The utility model is suitable for a biotechnology field.
Description
Technical field
The invention belongs to biological technical field.
Background technology
The external fertilization of animal, transgenic, clone etc. are a kind of important technical that animal carries out manual intervention breeding now, along with external fertilization culture technique, transgenic, the optimization of cloned animal technology and the raising of efficiency, most animals has all proceeded by the production of external fertilization breeding, transgenic and cloned animal now, but ovum growth course in early days is wrapped up by cumulus cell, in order to further investigate the developmental mechanism of oocyte and improve external fertilization, transgenic and cloned animal production effect, it is often necessary to remove cumulus cell.Existing cumulus cell removal device it is frequently necessary to mouth or hand control suction, easily cause pollution and the loss of oocyte, simultaneously, owing to the obstruct of small opening ware is when operating, and the position of oocyte cannot be clearly viewed, therefore easily causing cell damage, to affect the vigor of oocyte and oocyte long and have impact on the effect that continues test in the outdoor time of staying.
Summary of the invention
The utility model proposes a kind of device removing cumulus cell, it be that the cell in order to solve in the device culture dish in operation of existing removing cumulus cell is affected by environment greatly, easily affect cytoactive and the problem using air bag to provide pressure signal poor accuracy for piping and druming pin.
The device of removing cumulus cell described in the utility model, this device includes compression pump 1, airway 2, operation handle 3, piping and druming pin 7 and culture dish erecting device；
nullDescribed culture dish erecting device is columnar structured，Inner side and the bottom surface of described columnar structured sidewall are respectively and fixedly provided with heat-insulation layer，Sidewall is divided into two parts along sidewall bus by described cylindrical shape，A portion sidewall is fixed with columnar structured bottom surface，Another part sidewall all separates with columnar structured top disc and bottom disc，Described columnar structured top is provided with three layers of circular transparent sheet，The bottom surface of culture dish erecting device、Ground floor transparent thin board 11 and the partial sidewall being fixed on bottom surface constitute culture dish placed cavity 12，The edge of described ground floor transparent thin board 11 and third layer transparent thin board 9 is all fixing with columnar structured sidewall to be connected，The length opening more than 1/2nd thin plate arc length is had on sidewall between ground floor transparent thin board 11 and third layer transparent thin board 9，Second layer circular transparent sheet 10 is arranged between ground floor transparent thin board 11 and third layer transparent thin board 9，The edge of second layer circular transparent sheet 10 is provided with protruding handle，Described protruding handle is arranged on length on sidewall and is more than the opening part of 1/2nd thin plate arc length，And the lower surface of the described upper and lower surface of second layer circular transparent sheet 10 and the upper surface of ground floor transparent thin board 11 and third layer transparent thin board 9 fits tightly；
The signal output end mouth of compression pump 1 is connected with piping and druming pin 7 air inlet port by airway 2, and one end of described airway 2 is each attached to operate in handle 3 with the inlet end of piping and druming pin 7；
Being additionally provided with pressure regulation button 5 on operation handle 3, described pressure regulation button 5 produces the size of pressure for regulating compression pump 1.
This utility model uses compression pump to replace thinking the air bag of Non-follow control pressure, realize the accurate control to pressure, decrease the uneven pressure affecting air bag generation owing to exerting oneself in operating process, the problem causing cell damage, heat-insulation layer is set in the inner side of foster ware erecting device simultaneously, avoid the external temperature impact on culture dish inner cell in the operating process carrying out removing cumulus cell, affect the activity of cell, use three layers of transparent thin board, second layer transparent thin board is enterprising is provided only with a line manhole, when operating by Plane Rotation second layer transparent thin board, operate, decrease contacting of culture dish and outside air, sidewall at piping and druming pin is fixedly installed pinhole cameras simultaneously, handle arranges display screen, realization pushes away piping and druming pin operating side and observes clear.
Accompanying drawing explanation
Fig. 1 is the apparatus structure schematic diagram of removing cumulus cell described in the utility model；
Fig. 2 is the structural representation of the second layer transparent thin board described in detailed description of the invention one.
Detailed description of the invention
Detailed description of the invention one, combining Fig. 1 and Fig. 2 present embodiment is described, the device of the removing cumulus cell described in present embodiment, this device includes compression pump 1, airway 2, operation handle 3, piping and druming pin 7 and culture dish erecting device；
nullDescribed culture dish erecting device is columnar structured，Inner side and the bottom surface of described columnar structured sidewall are respectively and fixedly provided with heat-insulation layer，Sidewall is divided into two parts along sidewall bus by described cylindrical shape，A portion sidewall is fixed with columnar structured bottom surface，Another part sidewall all separates with columnar structured top disc and bottom disc，Described columnar structured top is provided with three layers of circular transparent sheet，The bottom surface of culture dish erecting device、Ground floor transparent thin board 11 and the partial sidewall being fixed on bottom surface constitute culture dish placed cavity 12，The edge of described ground floor transparent thin board 11 and third layer transparent thin board 9 is all fixing with columnar structured sidewall to be connected，The length opening more than 1/2nd thin plate arc length is had on sidewall between ground floor transparent thin board 11 and third layer transparent thin board 9，Second layer circular transparent sheet 10 is arranged between ground floor transparent thin board 11 and third layer transparent thin board 9，The edge of second layer circular transparent sheet 10 is provided with protruding handle，Described protruding handle is arranged on length on sidewall and is more than the opening part of 1/2nd thin plate arc length，And the lower surface of the described upper and lower surface of second layer circular transparent sheet 10 and the upper surface of ground floor transparent thin board 11 and third layer transparent thin board 9 fits tightly；
The signal output end mouth of compression pump 1 is connected with piping and druming pin 7 air inlet port by airway 2, and one end of described airway 2 is each attached to operate in handle 3 with the inlet end of piping and druming pin 7；
Being additionally provided with pressure regulation button 5 on operation handle 3, described pressure regulation button 5 produces the size of pressure for regulating compression pump 1.
Use the device of removing cumulus cell described in present embodiment, compared with the mode of existing employing mouth inspiration row piping and druming, it is to avoid be easily caused the problem that oocyte pollutes.
Detailed description of the invention two, present embodiment are further illustrating of the device to the removing cumulus cell described in detailed description of the invention one, and compression pump 1 uses portable pressure pump.
Portable pressure pump described in present embodiment is that portable hand grasps pneumatic supply, including providing pressure source when pressure pressure difference transmitter and pressure transducer.
The transmission medium of compression pump is air, buck is steady, use ultra-fine trimming part, ensure that sufficiently small fineness adjustment minimal adjustment fineness is 0.01pa, shell uses heat insulating material to suspect that heat-insulation and heat-preservation, greatly reducing the impact that minute-pressure instrument is verified by temperature, this compression pump have employed snap joint and the connecting line of Germany's import.Simple in construction, reliability height, convenient operating maintenance, be difficult to reveal.Regulator Cao Yong precision bulk container cavity volume piston, pressure regulation spectrum is big, and buck is steady.It is more beneficial for this utility model and is taken out during cumulus cell the requirement to pressure.
Detailed description of the invention three, present embodiment are further illustrating of the device to the removing cumulus cell described in detailed description of the invention one, and it also includes display screen 4, constant temperature regulation and control layer and temperature sensor；Display screen 4 is arranged on the outer surface of the sidewall of culture dish erecting device, constant temperature regulation and control layer and temperature sensor are equipped with the inner side of the heat-insulation layer of culture dish erecting device, described constant temperature regulation and control layer includes resistive heater, cooling piece and single-chip microcomputer, the power control signal input of described resistive heater connects the thermal control signals input of single-chip microcomputer, the power control signal input of cooling piece connects the refrigeration work consumption control signal outfan of single-chip microcomputer, described temperature sensor is for gathering the temperature signal in culture dish placed cavity 12, the signal output part of described temperature sensor is simultaneously connected with the signal input part of display screen 4 and the temperature signal input of single-chip microcomputer.
Detailed description of the invention four, present embodiment are further illustrating of the device to the removing cumulus cell described in detailed description of the invention three, and a limit of display screen 4 and being hinged at of culture dish erecting device, during use, the display surface of display screen 4 is upwards.
Display screen described in present embodiment is hinged on the side of culture dish erecting device, in use display screen is rotated vertical as the side of culture dish erecting device, to the temperature signal in the side observing culture dish erecting device in culture dish during convenient to operate, when not using, display screen is fitted in the side of culture dish erecting device.
Claims (4)
1. remove the device of cumulus cell, it is characterised in that this device includes compression pump (1), airway (2), manipulator
Handle (3), piping and druming pin (7) and culture dish erecting device；
Culture dish erecting device is columnar structured, and inner side and the bottom surface of described columnar structured sidewall are respectively and fixedly provided with heat-insulation layer,
Sidewall is divided into two parts, a portion sidewall and columnar structured bottom surface and cylinder along sidewall bus by described cylindrical shape
Shape structure top end is the most fixing, and another part sidewall all separates with columnar structured top disc and bottom disc, described cylindrical shape
The top of structure is provided with three layers of circular transparent sheet, the bottom surface of culture dish erecting device, ground floor transparent thin board (11) and solid
The partial sidewall being scheduled on bottom surface constitutes culture dish placed cavity (12), and described ground floor transparent thin board (11) and third layer are transparent
The edge of thin plate (9) is all fixing with columnar structured sidewall to be connected, transparent at ground floor transparent thin board (11) and third layer
The length opening more than 1/2nd thin plate arc length, second layer circular transparent sheet (10) is had on sidewall between thin plate (9)
It is arranged between ground floor transparent thin board (11) and third layer transparent thin board (9), second layer circular transparent sheet (10)
Edge is provided with protruding handle, and described protruding handle is arranged on length on sidewall and is more than the opening part of 1/2nd thin plate arc length, and
The upper and lower surface of described second layer circular transparent sheet (10) is saturating with the upper surface of ground floor transparent thin board (11) and third layer
The lower surface of bright thin plate (9) fits tightly；
The signal output end mouth of compression pump (1) is connected with piping and druming pin (7) air inlet port by airway (2), described
One end of airway (2) is each attached to operate in handle (3) with the inlet end of piping and druming pin (7)；
Being additionally provided with pressure regulation button (5) in operation handle (3), described pressure regulation button (5) is used for regulating compression pump
(1) size of pressure is produced.
The device of removing cumulus cell the most according to claim 1, it is characterised in that compression pump (1) uses portable
Formula compression pump.
The device of removing cumulus cell the most according to claim 1, it is characterised in that it also include display screen (4),
Constant temperature regulation and control layer and temperature sensor；Display screen (4) is arranged on the outer surface of the sidewall of culture dish erecting device, and constant temperature is adjusted
Control layer and temperature sensor are equipped with the inner side of the heat-insulation layer of culture dish erecting device, and described constant temperature regulation and control layer includes adding thermal resistance
Silk, cooling piece and single-chip microcomputer, the power control signal input of described resistive heater connects the thermal control signals of single-chip microcomputer
Input, the power control signal input of cooling piece connects the refrigeration work consumption control signal outfan of single-chip microcomputer, described temperature
Sensor is used for gathering the temperature signal in culture dish placed cavity (12), and the signal output part of described temperature sensor connects simultaneously
Connect signal input part and the temperature signal input of single-chip microcomputer of display screen (4).
The device of removing cumulus cell the most according to claim 3 a, it is characterised in that limit of display screen (4)
With being hinged at of culture dish erecting device, during use, the display surface of display screen (4) is upwards.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620347067.7U CN205528795U (en) | 2016-04-22 | 2016-04-22 | Device of desorption cumulus cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620347067.7U CN205528795U (en) | 2016-04-22 | 2016-04-22 | Device of desorption cumulus cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205528795U true CN205528795U (en) | 2016-08-31 |
Family
ID=56797672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620347067.7U Expired - Fee Related CN205528795U (en) | 2016-04-22 | 2016-04-22 | Device of desorption cumulus cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205528795U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967584A (en) * | 2017-05-04 | 2017-07-21 | 南昌大学 | A kind of automated cell suspension blows and beats evenly mixing device |
-
2016
- 2016-04-22 CN CN201620347067.7U patent/CN205528795U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967584A (en) * | 2017-05-04 | 2017-07-21 | 南昌大学 | A kind of automated cell suspension blows and beats evenly mixing device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203873915U (en) | Electronic double control semen collection device | |
| CN205528795U (en) | Device of desorption cumulus cell | |
| CN102161970A (en) | Portable cell incubator | |
| CN208947895U (en) | A kind of New Type of Intelligent Instrument protective device | |
| CN207047251U (en) | A kind of high pressure cell incubator | |
| CN203680506U (en) | Cement specimen maintenance truck | |
| CN110468043A (en) | A kind of Embryo Culture case | |
| CN206359537U (en) | Histocyte culture environment controls tape deck | |
| CN206498738U (en) | One plant growth climate box | |
| CN206238190U (en) | A kind of portable marine alga specimen making box | |
| CN206369559U (en) | A kind of USB interface-based equipment condition monitoring and fault location system | |
| CN221740273U (en) | Portable inoculation device for wheat powdery mildew | |
| CN216557999U (en) | Sample drying device for environmental monitoring laboratory | |
| CN113145026A (en) | Culture apparatus of single crystal and gel | |
| CN206486543U (en) | A kind of CO2gas incubator of medical mass cell culture | |
| CN221465515U (en) | Soil humidity early warning device of sand paddy field | |
| CN207330942U (en) | A kind of microbial teting box | |
| CN205413062U (en) | Portable biomedicine experiment box | |
| CN207610766U (en) | A kind of mounting structure of electronic scale pressure sensor | |
| CN216084671U (en) | Visualized and easy-to-operate control panel | |
| CN209449350U (en) | A kind of micro computer artificial climate device | |
| CN221710939U (en) | Novel hatching device of hermetia illucens | |
| CN205556691U (en) | Mould assembling of microbial cultivation inspection group | |
| CN206906913U (en) | A kind of internet culture dish management system | |
| CN205181361U (en) | Internal medicine clinical examination fixed bolster of taking a blood sample |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160831 Termination date: 20170422 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |