CN114680233A

CN114680233A - Application of cinnamaldehyde in preparing feed, feed additive, veterinary drug or water-drinking preparation for preventing or treating poultry adenoid myogastritis

Info

Publication number: CN114680233A
Application number: CN202011591319.8A
Authority: CN
Inventors: 周建成; 吴创
Original assignee: Liuyang Lebai Organic Nutrition Co ltd
Current assignee: Liuyang Lebai Organic Nutrition Co ltd
Priority date: 2020-12-29
Filing date: 2020-12-29
Publication date: 2022-07-01

Abstract

The invention provides an application of cinnamaldehyde in preparing feed, feed additive, veterinary drug or water-drinking preparation for preventing or treating poultry glandular gastritis and glandular stomach erosion. According to the invention, the cinnamaldehyde is added into the feed, the feed additive, the veterinary drug or the drinking preparation, so that the poultry adenomuscular gastritis and the adenomuscular stomach erosion can be effectively prevented and treated, and the use of antibiotics is avoided.

Description

Application of cinnamaldehyde in preparing feed, feed additive, veterinary drug or water-drinking preparation for preventing or treating poultry adenomuscular gastritis

Technical Field

The invention belongs to the technical field of livestock and poultry breeding, and particularly relates to an application of cinnamaldehyde in preparation of feed, feed additives, veterinary drugs or water-drinking preparations for preventing or treating poultry adenofibromyositis.

Background

Since poultry glandular myositis and muscular stomach erosion were first reported in the netherlands in 1978, the diseases occur all over the world, which brings great harm to the breeding industry and cannot be ignored. At present, cases of infectious proventriculitis frequently occur in the market, the treatment is difficult, and the loss is not small for the breeding industry. Almost all chicken flocks with proventriculitis can separate viruses, and the viruses widely occur in northeast, north China and south China.

Proventriculitis and muscular stomach erosion are common diseases of poultry, particularly broiler chicken and breeding hens, which are frequently accompanied with the diseases, the morbidity is 7-28%, the morbidity and mortality are 30-50%, the course of disease is long, the death and culling rate is high, and the incidence rate is in an ascending trend, so that great loss is caused. In a word, the adenomyositis (erosion) is a syndrome with complex causes, about 40 percent of which is caused by the mildew of a large amount of aged grains, feed raw materials and finished products; the other 40 percent of chickens and farms are infected with various viruses, including hatching embryonated chicken eggs with viruses to enable the viruses to infect the chickens and the farms one generation after another; if the virus is gonad-myositis, the disease is developed in one chicken farm, and almost 100% of the next batch is also developed; the rest 10 percent of the feed is caused by oxidation of feed raw materials, and the other 10 percent of the feed is poor in feeding management, such as poor ventilation, air moisture in a chicken house, musty and strong ammonia flavor, mycotoxin poisoning, massive proliferation of anaerobic bacteria in vivo, glandular and gastric dysfunction, increase of gastric acid secretion, glandular and gastric ulcers and muscular and gastric erosion.

The disease chicken with the adenoid gastritis is clinically mainly manifested by growth retardation, even stagnation, mental depression, disharmony of feather and fin drooping. Some patients have lacrimation and swollen eyes with respiratory symptoms, discharge white or green thin feces and have excessive stool. The scraping examination shows that the disease chicken has swollen glandular stomach like a ball, is milk white, has erosive bleeding and hyperplasia of glandular stomach wall, has ulcer focus, swollen and bleeding nipple, fuses between glandular stomach and muscular stomach nipple, has zonal bleeding and has no limit. The muscular stomach, thymus and spleen are obviously atrophied. The intestinal mucosa is fallen off, the intestinal wall is thin, and bleeding is caused to different degrees. Referring to fig. 1, fig. 1 is a photograph of a glandular myostomach of a sick chicken dissected for typical glandular myogastritis.

For the prevention and treatment of the proventriculitis, an effective treatment method has not been found for a long time, and the method is a worldwide problem, and in the prior art market, for the proventriculitis and the myogastric erosion, feed mould removal and western medicines are mainly adopted to control bacterial infection and inhibit gastric acid secretion, so that the treatment effect is poor. Patents and documents are not few, and a Chinese herbal medicine formula such as rhizoma cyperi, rhizoma atractylodis macrocephalae and cortex moutan is used for preventing and treating the adenomyositis, but the Chinese herbal medicine formula has no exact effect and is difficult to be widely used; the other type uses western medicines, such as cimetidine, aspirin, dimetridazole, amoxicillin, omeprazole sodium and the like, which not only have no stable effect and are widely used, but also use antibiotics and medicines which are used together with people, and the unsafe meat, eggs and milk are produced to cause adverse effects on the health of people. The western medicine formula patents include: penicillin, streptomycin (CN 10466429A); omeprazole (CN 107397719A); florfenicol, metronidazole, pyritinidine hydrochloride (CN 101966178A); new hope group terbinafine hydrochloride, ampicillin (CN 103394021B); gentamicin, pyritinidine (CN 101647883A); cimetidine, dimetridazole, amoxicillin, potassium citrate (CN 102983363A); amoxicillin, astragalus polysaccharides (CN 104208089A); industrial salts of penicillin, ranitidine (CN 102283886A); amoxicillin, mold remover (CN107789362A), and the like.

Patent application 201310629029.1 discloses a composition for treating chicken proventriculitis and a preparation method thereof, wherein the composition comprises the following components in parts by weight: 5-10 parts of penicillin, 5-10 parts of streptomycin, 10-3 parts of ash bark, 10-30 parts of montmorillonite and 10-30 parts of endothelium corneum gigeriae galli. Patent application 201510057201.X 'A compound preparation for treating broiler proventriculitis' comprises the following components in parts by weight: 1-5 percent of proton pump inhibitor, 5-30 percent of vitamin U, 5-30 percent of colloidal bismuth pectin and 50-91 percent of bulking agent. Wherein the proton pump inhibitor is one or more of omeprazole, rabeprazole, pantoprazole, esomeprazole, lansoprazole or salts corresponding to the proton pump inhibitor; the filler is one or more of medicinal fillers such as starch, glucose, lactose, maltose, dextrin, etc. Patent application 201510666591.0 discloses a traditional Chinese medicine oral liquid for treating chicken proventriculitis and muscular gastritis, which comprises the following raw materials in parts by weight: 20-60 parts of coptis chinensis, 50-80 parts of scutellaria baicalensis, 30-50 parts of gardenia, 10-30 parts of common andrographis herb, 10-30 parts of Chinese pulsatilla root, 8-20 parts of liquorice, 8-15 parts of wrinkled gianthyssop herb, 8-15 parts of bighead atractylodes rhizome, 8-15 parts of rhizoma atractylodis, 8-15 parts of mangnolia officinalis, 8-15 parts of poria cocos, 8-15 parts of fructus forsythiae, 8-15 parts of radix bupleuri, 8-15 parts of platycodon grandiflorum, 8-15 parts of dried orange peel and 8-15 parts of pericarpium arecae, and the raw materials are extracted by ethanol and decocted by water to prepare the traditional Chinese medicine oral liquid.

In the prior art, either antibiotic medicines are taken as main medicines or pure Chinese herbal medicines are combined to form complex components, so that the feed is not enough in the comprehensive consideration of feeding safety and efficacy. The feed additive catalogue in China requires definite components, is convenient to use, can be detected and traced, obviously cannot be realized by the prior art, is difficult to use in a feed factory, and can cause proventriculitis frequently in laying hens, the laying period is forbidden to use antibiotics and related products, even if the laying hens are used by theft, the laying hens can remain in eggs, and once the laying hens are found out, the punishment is quite serious.

Disclosure of Invention

In view of the above, the technical problem to be solved by the present invention is to provide an application of cinnamaldehyde in preparing a feed, a feed additive, a veterinary drug or a water-based preparation for preventing and treating poultry adenoid gastritis and adenoid gastric erosion, wherein the cinnamaldehyde can be added into the feed, the feed additive, the veterinary drug or the water-based preparation for effectively preventing and treating poultry adenoid gastritis and adenoid gastric erosion, thereby avoiding the use of antibiotics.

The invention provides an application of cinnamaldehyde in preparing feed, feed additive, veterinary drug or water-drinking preparation for preventing or treating poultry adenomuscular gastritis.

Preferably, when the feed additive or veterinary drug is added into the feed, the concentration of the cinnamaldehyde in the feed is 200-5000 mg/kg;

the concentration of cinnamaldehyde in drinking water prepared by the drinking water preparation is 20-2500 mg/kg.

Preferably, when the feed additive, the veterinary drug or the drinking water preparation is used for preventing poultry adenomuscular gastritis and adenomuscular stomach erosion, the concentration of cinnamaldehyde added into the feed is 250-500 mg/kg, or the concentration of cinnamaldehyde in drinking water prepared by the drinking water preparation is 20-250 mg/kg, and the using method is as follows: the preparation is used for 5-12 days.

Preferably, the using method comprises the following steps: the medicine is used for 5-12 days, and is used once after 15-45 days, and the medicine is used for 3-25 days once.

Preferably, the using method comprises the following steps: the medicine is used for 5 to 12 days, and is used once after every 20 to 25 days, and the medicine is used for 3 to 5 days.

Preferably, when the feed additive, the veterinary drug or the drinking water preparation is used for treating poultry adenomuscular gastritis and adenomuscular gastric erosion, the concentration of cinnamaldehyde in the feed is 350-1250 mg/kg when the feed is mixed with the feed, or the concentration of cinnamaldehyde in drinking water prepared by the drinking water preparation is 75-625 mg/kg, and the using method is as follows: the preparation is used for 3-15 days.

Preferably, the using method comprises the following steps: the medicine is used for 3-15 days, and is used once after every 10-30 days, and the time is 3-15 days.

Preferably, the poultry is laying poultry in the egg laying period, when the feed additive or veterinary drug is used for mixing with feed, the concentration of cinnamaldehyde in the feed is 75-250 mg/kg, or the concentration of cinnamaldehyde in drinking water prepared by a drinking water preparation is 37.5-125 mg/kg, the using time is 8-30 days/time, the cinnamaldehyde is used once every 20-45 days, and the cinnamaldehyde is used once every 8-15 days, so that the health of laying hens is ensured.

Preferably, the feed, feed additive, veterinary drug or drinking water preparation further comprises an auxiliary component for preventing or treating poultry adenomyositis and adenomyositis erosion, wherein the auxiliary component comprises plant essential oil, Chinese herbal medicine or chemical composition.

Preferably, the plant essential oil is selected from carvacrol, thymol or eugenol.

Compared with the prior art, the invention provides the application of cinnamaldehyde in preparing feed, feed additives, veterinary drugs or water-based preparations for preventing or treating poultry glandular myositis and glandular stomach erosion. According to the invention, the cinnamaldehyde is added into the feed, the feed additive, the veterinary drug or the drinking preparation, so that the poultry adenomuscular gastritis and the adenomuscular stomach erosion can be effectively prevented and treated, and the use of antibiotics is avoided.

The results show that in order to ensure effective defense and treatment of glandular gastritis (erosion), the addition concentration of the feed needs 250-500 ppm (when egg-laying poultry generates the glandular gastritis in the egg laying period, in order to not affect the egg laying and egg quality, the cinnamaldehyde concentration in the feed can be as low as more than or equal to 75mg/kg), the severe glandular gastritis is caused to virulent viruses, 1000-2000 ppm needs to be added into the feed, and the removal of diseases by hands can be basically achieved.

The above description is a scheme for preventing and treating the adenomyosis which is confirmed or obtained by experience, the concentration of the cinnamaldehyde in the feed must reach 200-5000mg/kg, but because the cause of the adenomyosis is complicated, we do not exclude that the low dose of 20-150mg/kg added in the feed or drinking water has a certain effect on preventing the adenomyosis.

Drawings

FIG. 1 is a photograph of a glandular myostomach after dissection of a diseased chicken with typical glandular myogastritis;

FIG. 2 is a photograph of dissected adenomyosis of broiler chickens of example 6 control group;

FIG. 3 is a photograph of dissected adenomyosis of broiler chickens of example 6 three experimental groups;

FIG. 4 is a photograph of the dissected adenomyosis of the chicken of example 8;

FIG. 5 is a photograph of a farm according to example 9;

FIG. 6 is a photograph of the dissected adenomyosis of the laying hen of example 9;

FIG. 7 is a photograph of a flock of chickens according to example 10 after using the product of the invention;

FIG. 8 is a photograph of a chicken farm of example 12 after using the product of the present invention;

FIG. 9 is a photograph of a chicken farm of example 13 after use of the product of the present invention;

FIG. 10 is a graph of the symptoms of the cesarean-section myogastritis of the chicken of example 14;

FIG. 11 is a graph showing the muscular gastritis symptoms at the section of the chicken flocks of example 14;

FIG. 12 is a graph showing the symptoms of the cesarean-section myogastritis of the chicken group of example 15;

FIG. 13 is a graph showing the symptoms of the cesarean-section myogastritis of the chicken group of example 15;

FIG. 14 is a graph showing the symptoms of the cesarean-section myositis of the chicken group in example 23.

Detailed Description

The invention provides an application of cinnamaldehyde in preparing feed, feed additive, veterinary drug or water-drinking preparation for preventing or treating poultry adenoid myogastritis.

In the invention, the concentration of the cinnamaldehyde in the feed is 250-5000 mg/kg, or the concentration of the cinnamaldehyde in the drinking water prepared by the drinking water preparation is 20-2500 mg/kg,

when the feed, the feed additive, the veterinary drug or the drinking water preparation is used for preventing poultry adenomyosis and adenomyosis erosion, the concentration of the cinnamaldehyde added into the feed is 250-500 mg/kg, or the concentration of the cinnamaldehyde in the drinking water prepared by the drinking water preparation is 20-250 mg/kg, and the using method comprises the following steps: the composition is used for 5-20 days.

In some preferred embodiments of the present invention, the method of use is: the medicine is used for 5 to 20 days, and is used once after 15 to 45 days, and the medicine is used for 3 to 20 days once. Can ensure that almost 100 percent of newly-entered chicken groups and duck groups which can suffer from the adenomyositis in the polluted breeding farm which originally suffers from the adenomyositis and infected poultry seedlings which are derived from the breeding farm with the adenomyositis do not suffer from the adenomyositis any more.

In other preferred embodiments of the present invention, the method of use is: the medicine is used for 5-12 days, and is used once after 15-45 days, and the medicine is used for 3-12 days once. Can ensure that almost 100 percent of newly-entered chicken groups and duck groups which can suffer from the adenomyositis in the polluted breeding farm which originally suffers from the adenomyositis and infected poultry seedlings which are derived from the breeding farm with the adenomyositis do not suffer from the adenomyositis any more.

When the feed, the feed additive, the veterinary drug or the drinking water agent is used for treating poultry adenomyositis and adenomyositis erosion, the concentration of the cinnamaldehyde in the feed is 350-1250 mg/kg when the feed is mixed and fed, or the concentration of the cinnamaldehyde in the drinking water prepared by the drinking water preparation is 75-625 mg/kg, and the using method is as follows: the preparation is used for 6-15 days. The death and culling rate is 1-2%.

In other preferred embodiments of the present invention, the method of use is: the preparation is used for 6-15 days, and is used once after every 10-25 days, and the preparation is used for 6-15 days once. The death and culling rate is 1-2%.

The traditional Chinese medicine composition is used for preventing the adenomyositis of the population with a large risk of the adenomyositis for every 15-45 days.

For young chickens and poultries which do not lay eggs, the prevention and treatment of the adenomyositis can be carried out according to the using method.

When the poultry is laying poultry in the egg producing period and is mixed with feed, the concentration of the cinnamaldehyde in the feed is 75-250 mg/kg, or the concentration of the cinnamaldehyde in a drinking water preparation is 37.5-125 mg/kg, the using time is 8-30 days/time, the cinnamaldehyde is used once every 20-45 days, and the cinnamaldehyde is used once every 8-15 days, so that the health of the laying hens is ensured.

In some embodiments of the present invention, the feed, feed additive, veterinary drug or drinking water preparation further comprises an auxiliary ingredient for preventing or treating poultry adenomyositis and adenogastral erosion, wherein the auxiliary ingredient comprises plant essential oil, Chinese herbal medicine or chemical composition. Wherein the plant essential oil is selected from carvacrol, thymol or eugenol.

According to the invention, the cinnamaldehyde is added into the feed, the feed additive, the veterinary drug or the drinking preparation, so that the poultry adenomuscular gastritis and the adenomuscular stomach erosion can be effectively prevented and treated, and the use of antibiotics is avoided.

The above description is a scheme for preventing and treating the adenomyosis which is confirmed or obtained by experience, the concentration of the cinnamaldehyde in the feed must reach 200-5000mg/kg, but because the cause of the adenomyosis is complicated, we do not exclude that the low dosage of 25-150mg/kg added in the feed or drinking water has a certain effect on preventing the adenomyosis.

For further understanding of the present invention, the following examples are provided to illustrate the application of cinnamaldehyde provided in the present invention in preparing feed, feed additive, veterinary drug or water-based formulation for preventing or treating poultry adenofibromyositis and adenofibromyalgia erosion, and the scope of the present invention is not limited by the following examples.

Example 1

White carbon black is used for adsorbing cinnamyl aldehyde to prepare powdery or spherical feed additive containing 25 percent of cinnamyl aldehyde.

Example 2

Preparing a veterinary drug preparation containing 25% of cinnamaldehyde by using an emulsifier (Tween) at 80 ℃, water and cinnamaldehyde;

the veterinary drug preparation can be diluted into uniform liquid by being mixed with water to obtain a drinking water preparation.

Example 3

The influence of cinnamaldehyde on various production performances such as chicken weight gain, feed conversion ratio, feed intake and the like is evaluated by healthy chicken flocks, and the condition of replacing antibiotics is analyzed:

960 Chongqing Daaiyin white feather cocks were selected and randomly divided into 12 groups for blind test, each group had 8 replicates, and each replicate 10. The cinnamaldehyde products produced in example 1 were added to negative control basal diets (formula shown in table 2 below), concentrations and groups of antibiotics and cinnamaldehyde were shown in table, infrared lamps were used to maintain the temperature three days before night, natural temperature was used later, the ground was kept flat, the henhouse was modified from a pigsty, and 9.2019, 5.5.5.a.m.: and (3) weighing the chicken on an empty stomach at a ratio of 00-11: 00, feeding the chicken for 41 days, and weighing the chicken on an empty stomach at a ratio of 5: 00-11: 00 in 10 months and 16 days in the morning, wherein the results are shown in a table 1:

TABLE 1

And (4) conclusion: from table 1, the feed added with 50-500 mg/kg of cinnamaldehyde is close to or slightly superior to the antibiotic Virginiamycin which is recognized by the industry to have the best growth promoting effect.

The cinnamic aldehyde is preferably 100mg/kg, the daily gain is improved by 9.19 percent compared with the negative control, and the appropriate addition amount of the cinnamic aldehyde in the daily ration of the broiler is 50-125 mg/kg; the high dose groups 250 and 500mg/kg seem to have slightly lower feed intake than the low dose groups 50-125mg/kg, but the weight gain and feed conversion rate are slightly affected, even not affected, so that the addition range of the cinnamaldehyde in the daily ration is 75-2000 ppm, the short-term 7-15 days of prevention and treatment has no influence or little influence on the growth of poultry, but the long-term addition and use of the high dose of more than or equal to 200mg/kg are not recommended, and the high dose of cinnamaldehyde is reported to cause the growth inhibition of osteoblasts and needs to be further proved and researched. When the common poultry is used for promoting growth by replacing antibiotics, the adding concentration of the cinnamaldehyde in the feed is 25-100 mg/kg.

Example 4

Animal experiments: 144 healthy AA broiler chickens of 1 day old were selected and randomly divided into 2 treatments, 6 replicates each, and 12 chickens each.

The treatment group 1 is a blank group and is fed with basic ration;

the treatment group 2 was a test group, in which 1.5kg/T of cinnamaldehyde produced in example 1 was added to the base ration at a cinnamaldehyde addition concentration of 375 mg/kg.

The test period was 42 days. During the test, the feed and the remainder were recorded in units of each replicate group, from which the average daily gain, daily feed intake and feed-to-weight ratio were calculated.

After the test is finished, 3 test chickens close to the average weight are randomly selected from each repeating group, blood is collected from veins under wings by 10mL, serum is separated and stored at the temperature of 20 ℃ below zero for determining the content of the immunoglobulin. After blood collection, jugular vein bloodletting is slaughtered, spleen, thymus, bursa of Fabricius and other organs are picked, adipose tissues are removed, and fresh weight is quickly weighed and used for calculating immune organ indexes. The composition and nutritional ingredients of the basal diet are shown in table 2.

TABLE 2 basal diet formula and nutritional level

Raw materials	Content (wt.)	Nutritional levels	Numerical value
				Corn (corn)	58.2	Metabolizable energy (kcal/kg)	3000
Bean pulp	29.1	Crude protein (%)	21.5
				Cottonseed protein	4.0	Calcium (%)	0.92
Corn protein powder	3.0
				Stone powder	1.1	Phosphorus (%)	0.45
Salt	0.36	Lysine (%)	1.10
				Calcium hydrogen phosphate	2.1	Egg + cysts (%)	0.85
Methionine	0.12
				Lysine	0.02
Soybean oil	1.0
				1% premix	1

TABLE 3 Effect of the feed additives of the invention on broiler productivity

	Treatment group 1	Treatment group 2
			Average daily gain (g)	52.43±1.43^a	55.29±1.23^b
Average daily food intake	95.28±0.83^a	95.55±0.77^b
			Material to weight ratio	1.82±0.05^a	1.73±0.03^b

Significant difference in different letter representations of the same row

As can be seen from the data in Table 3, the average daily gain, feed intake and feed conversion ratio of treatment group 2 were improved by 5.5%, 0.3% and 4.9%, respectively.

TABLE 4 influence of the feed additive of the invention on the immune organ index of broilers

	Treatment of group 1	Treatment of 2 groups
			Spleen index (g/kg)	1.36±0.04^a	1.59±0.12^b
Thymus index (g/kg)	3.30±0.13^a	3.56±0.55^b
			Bursa index (g/kg)	1.52±0.08^a	1.75±0.09^b

Significant difference in different letters in the same row

As can be seen from the data in Table 4, the spleen index, thymus index and bursal index of the treated group 2 were all increased by 16.9%, 7.9% and 15.1%, respectively.

TABLE 5 Effect of the feed additives of the present invention on serum immunoglobulins of broilers

	Process 1	Treatment 2
			IgG(g/l)	2.52±0.06^a	2.79±0.08^b
IgA(g/l)	1.13±0.15^a	1.49±0.12^b
			IgM(g/l)	1.71±0.15^a	1.88±0.09^b

Significant difference in different letters in the same row

As can be seen from the data in Table 5, the IgG, IgA and IgM concentrations in treatment group 2 were all increased by 10.7%, 31.9% and 9.9%, respectively.

By combining the indexes, the cinnamaldehyde feed additive provided by the invention can greatly improve the production performance of the broiler chicken and can remarkably enhance the immune function of the broiler chicken.

Example 5

600 white feather broilers with 30-day-old diseased and toxic proventriculitis and no death are selected and randomly divided into 3 treatments, each treatment is divided into 5 repetitions, and each repetition comprises 40 chickens.

Treatment 1 control 1 was the basal diet group,

treatment 2 control 2 is a group of commercially available antibiotics (mainly containing terbinafine hydrochloride and ampicillin) for treating proventriculitis of a new company, which is added according to a label and simultaneously fed with bismuth potassium citrate in proper amount.

The treatment 3 is a test group, 2kg/T of the cinnamaldehyde product produced in example 1 is added into the basic ration, the adding amount of the cinnamaldehyde is 500mg/kg, and the death number and the death rate of the chickens in each treatment group are counted after the chickens are fed for 15 days.

TABLE 6 therapeutic effect of the feed additive of the present invention on proventriculitis of broiler chicken

	Control 1 group	Control 2 group	Treatment of 3 test groups
				Death only counts (only)	114±1.32^a	87±1.06^b	2.00±0.02^c
The mortality rate is%	57.00±0.66^a	43.5±0.06^b	1.0±0.05^c

Significant difference in different letters in the same row

As can be seen from the data in Table 6, the death number and death rate of the chickens treated by adding the cinnamaldehyde feed additive are far lower than those of the chickens treated by adding the cinnamaldehyde feed additive in two control groups, so that the feed additive has a good curative effect on proventriculitis and myogastritis, the curative effect of the feed additive is remarkably superior to that of conventionally used antibiotic drugs, and the feed additive is safer and more stable than the antibiotic drugs.

In conclusion, feeding experiments prove that the feed additive can obviously improve the production performance of the broiler chicken and obviously improve the immune function of the broiler chicken, thereby reducing the occurrence of the chicken with the gastritis and the proventriculitis, having good curative effect on the chicken with the gastritis and the proventriculitis, reducing the death rate and reducing the number of dead chickens.

Example 6

In the experiment for treating the gallinaceous proventriculitis, 4 experimental groups are set, the experiment 1-4 groups adopt the cinnamaldehyde product produced in the example 1, 500, 1000, 1500 and 2000mg/kg are sequentially added into basic daily ration, and a 5 th group of control groups adopt the basic daily ration. Feeding for 12 days.

The modeling experiment method of the sick chicken comprises the following steps:

preparing a mildew feed: under the conditions of 28 ℃ and 85% humidity, the water content of the feed is 25%, the feed is turned and mixed for 2 times per day for 4 consecutive days, and the feed containing the aflatoxin B1 is obtained for standby after drying, and the content of the obtained pathogenic aflatoxin B1 is 65.29 ug/kg;

secondly, the broiler chickens continuously eat the moldy feed for 10 days, and when the broiler chickens have proventriculitis, the modeling is successful.

Through comparison, the tests 1-4 groups mainly carry out comparison and determination through three indexes, namely, production performance indexes (average daily feed intake, average daily gain and feed conversion ratio), immune indexes (immunoglobulin content) and morphological indexes (the shapes of chicken stomachs and glandular stomachs).

The results are shown in figures 2 to 3, figure 2 is a photograph of the dissected glandular stomach of the broiler chicken of the control group in example 6, and the dissected figure 2 shows that the broiler chicken of the control group 5 has glandular stomach swelling and sarcogastric erosion which are typical symptoms of chicken proventriculitis. FIG. 3 is a photograph of dissected adenomyosis of broilers of three experimental groups of example 6, and the dissected adenomyosis of the three experimental groups of 500, 1000 and 1500mg/kg shown in FIG. 3 are normal and have no lesion. In FIG. 3, the glandular muscular stomach was dissected from three test groups of 500, 1000, and 1500mg/kg in order from left to right.

The results of the production performance index and the immunity index after the multiple comparisons are shown in tables 7 and 8:

TABLE 7 comparison of the Performance of broiler chickens in each group

TABLE 8 broiler immunoglobulin comparison (μ g/mL) of groups

	Test 1 group	Test 2 groups	Test 3 groups	Test 4 groups	Control group 5
						IgG	2923.65±0.29	3026.13±0.23	3567.29±0.12	3489.26±0.22	2735.80±0.98
IgM	823.24±0.66	929.35±0.73	1079.57±0.44	998.63±0.37	795.47±0.63
						IgA	361.64±0.33	408.34±0.32	436.53±0.42	457.33±0.55	319.37±0.12

The experiment result shows that 500-2000mg/kg of the feed can be added to effectively treat proventriculitis of chickens caused by aflatoxin B1, and intestinal health of broilers can be effectively improved. The growth performance of 500mg/kg broiler chicken is optimal, the feed intake and the weight gain are reduced to some extent along with the increase of the cinnamaldehyde concentration, but the feed conversion rate is improved more and more, and the contents of IgG, IgM and IgA can be improved by 500mg/kg, 1000 mg/kg, 1500mg/kg and 2000mg/kg of cinnamaldehyde. The daily gain and the feed conversion rate of the control group are far lower than those of each test group, the feed intake is small, the overfeeding phenomenon is serious, and the feed loss is as high as 21.81%.

The test conclusion shows that 500-2000mg/kg of feed can effectively treat the proventriculitis of the chicken caused by the mould, and all production performances are far better than those of a control group, even though the initial weight of 479.36 g of the control group 2 is far heavier than 426.54-429.73 g of the test group 1-4, and the health condition is good.

Example 7

More than 20 million white feather broilers in a chicken farm in 4 months in 2019 of Xinjiang Taiquan group Limited company have proventriculitis, and the reaction is serious, 600mg/kg (containing 150mg/kg of cinnamaldehyde) of the cinnamaldehyde feed additive produced in the embodiment 1 is added into each ton of feed, so that the reaction is basically ineffective, and the fact that the feed with the cinnamaldehyde concentration lower than 150mg/kg is basically ineffective in preventing and treating the serious white feather broilers with the proventriculitis is shown.

Example 8

The Weifang city of Shandong province has been imitatied, 26384m, a prefecture-integrated agriculture development Limited company, Law: some Liu, 134 is a domestic contract chicken company, which provides breeders with chicks, feeds and poultry drugs and recovers chickens, 30 thousand chickens are 817 chickens per month, 120 thousand chickens are eaten as big meat, all bred chickens have adenomyositis from 2019, 10 months to the present, and after dissection, see figure 4, and as can be seen from figure 4, the glandular stomach is congested and swollen, and the sarcogenic stomach becomes black and erosive:

all the chickens fed 2000 g of the feed additive containing 25% of cinnamaldehyde produced in example 1 per ton of the feed are recovered to feed after 10 days of continuous use, and the adenoid gastritis symptom disappears completely. Farmers would like this product very much.

The client contract chicken is changed from 11 months in 2019, the feed additive containing 25% of cinnamaldehyde and produced in example 1 is added into the feed, each ton of the feed is mixed with 1500 g of the feed for 10 days, and the glandular myositis does not occur in the later growth process of all chicken groups. The product becomes a necessary product for the same agriculture development company of \26384m.

Example 9

37075of city of lotus, Shandong province, Huang Anzhen of city, 186 x, hen breeding, 2 ten thousand hen breeding, see FIG. 5, and FIG. 5 is a photograph of the farm in example 9. The farmer and the chickens which have been laying at the peak of egg production in 5 months of 2020 have adenomyositis, the feed intake is reduced, the egg production is reduced by 10%, the chickens which have little feed intake and are in mental depression are dissected, the adenomyositis stomachs are as shown in figure 6, and the adenomyositis stomachs are obviously swollen.

The feed contains 25% of cinnamaldehyde produced in example 1, 500 g (125 mg/kg) of cinnamaldehyde is added to each ton of feed, the feed is continuously used for 10 days, the feed intake is recovered to a normal level, the egg laying is recovered to a normal level, and the chicken flock is recovered to a normal production.

Example 10

Shandong chatting about persimmon garden Zhennanguo, one of the grand, 156, each of the farmers, 8 thousands of meat in each batch, severe proventriculitis developed in 2019 in 2-9 months, from 11 months to the present in 2019, 25% cinnamaldehyde in the feed opening prepared in example 1, 1500 g cinnamaldehyde in each ton of feed, 10 days of continuous use, a total of 5 batches of 40 ten thousand 817 thousand chickens used, and the chickens of the farmers have no proventriculitis, and the chicken flocks are all healthy as shown in FIG. 7.

Example 11

The method comprises the steps of culturing laying hens in Shandong province Yizishi 37075and Cheng county Huang Anzhen, culturing a certain Guo, 138 Yu xi, culturing the laying hens, breeding 1 ten thousand young chickens, enabling the young chickens to have adenoid gastritis and decrease the feed intake, adding the cinnamaldehyde product containing 25% of the cinnamaldehyde product produced in example 1 into the feed in the year 2020 and 5 months, continuously using the feed for 10 days, recovering the feed intake to a normal level, and recovering the normal growth of young chicken flocks.

Example 12

Anhui Hefei Mao Yuan company (business person: Luo Jing 182X)

2 ten thousand 817 chickens are used by a farmer in Biqian town of Lanxi county, Xuancheng City, 8 months in 2020, 2kg of the product produced in example 1 is added into each ton of feed after opening the feed, the feed is continuously used for 10 days, and the glandular myositis does not occur in the whole chicken group within 30 days. Referring to fig. 8, fig. 8 is a photograph of the chicken farm of example 12 after using the product of the present invention.

Example 13

Some 135 x y of the city of commercially available commercial beans in Henan

In the persimmon garden of Liuzhaicun bean collected in Yongcheng county of Shang city, Henan province, the company has 2 ten thousand 817 chickens, 2kg of the cinnamaldehyde product containing 25% produced in example 1 is added to each ton of feed from the starter feed, the feed is continuously used for 10 days, and the whole chicken flock grows at the later stage without adenoid gastritis. Referring to fig. 9, fig. 9 is a photograph of the chicken farm of example 13 after using the product of the present invention.

Example 14

In the individual breeding of Yuanmen country peach source village in Yuanyang county, Xinxiang city, Henan province, 183X Y from Kudzuvine had feed intake of 5000 chickens (10 days old) only 50% of normal level, and the autopsy for the symptoms of the muscular gastritis is shown in figure 10, as shown in figure 10, the swelling of glandular stomach is large, and as shown in figure 11, the erosion of muscular stomach, the stratum corneum is almost perforated, and the diagnosis is confirmed.

2 kg/ton of the cinnamaldehyde product containing 25% produced in example 1 was mixed with the feed, feeding was recovered by more than 70% on the third day, 99% of the chickens returned to the normal feeding state on the fifth day after use, 12 chickens died in total, the anatomically recovered chickens had disappeared the proventriculitis symptom. However, 1% of the chickens had almost stopped feeding, had the symptoms of proventriculitis when dissected and were rejected. The previous drugs for treating proventriculitis with antibiotics have no effect.

Example 15

8000 chickens (22 days old) of 817 from Yuanmen county Yuanyang city, Xinxiang province (138 x) feed intake is 50% of normal amount before use, proventriculitis is serious, the state of the whole chicken group is not good, and the dissected dead chicken have cracked and damaged glandular stomach cuticle, and the glandular stomach hyperemia and swelling and the sarcomere of the sarcomere completely fall off in a figure 12.

The feed is mixed with 1 kg/ton of the product containing 25% of cinnamyl aldehyde produced in example 1, feeding is recovered to 80% after 3 days after use, 98% of chickens recover to a normal feeding state after 5 days after use, 16 chickens die in total, the glandular gastritis symptoms of the anatomically recovered chickens disappear, and only very few mild myogastritis symptoms exist. But less than 2 percent of the chickens fed by the feed are almost stopped, the symptoms of the anatomical proventriculitis are obvious, the chickens dissected and recovered on the 8 th day can not find the symptoms of the muscular gastritis any more, and the chickens hardly fed by the feed have the symptoms of the proventriculitis and the muscular gastritis.

Example 16

In 6 late 6 months in 2018 in Hunan Xiangjia animal husbandry, nearly 3 million chickens in a chicken farm of a generation of farmers are abnormally fed, the spirit is not good and proventriculitis is suspected, and the autopsy proves that 800 g/t (200 mg/kg of cinnamaldehyde) of the cinnamaldehyde feed additive produced in example 1 is fed into the feed from 28 days 6 months, and the reaction and the feed intake of the farmers are improved and the growth is normal after 10 days 7 months.

In the first 7 th month, the company has a breeding farm with twenty thousand chickens and thirty thousand chickens, proventriculitis is confirmed by a caesarean section, 1000 g/ton (250 mg/kg) of the cinnamaldehyde feed additive produced in example 1 is mixed in the feed, and the mixture is mixed for 12 days and is completely recovered to be normal.

Example 17

In a 20-million chicken farm with proventriculitis occurring in 12 months and 1 day in 2019 and belonging to an economic adult feed factory of five-channel Anhui in the economic development district of Union Town in the fixed town county of Union Town City, 300kg of the cinnamaldehyde feed additive produced in example 1 is provided for free, the cinnamaldehyde feed additive is received in 3 days in 12 months and 3 days in 2019 at a ratio of 20:31 seconds (from the well of the consignee), 1.0 kg/ton of the cinnamaldehyde feed additive is added into the feed at night, and all chickens are normal after 15 days of administration.

Example 18

In 2019, 20 days in 4 months, proventriculitis and enteritis commonly occur to broiler users (hope six and Sunday) of Shandong Weifang Xinrong feed Limited company (hope six and Sunday) due to seasonal reasons, the antibiotic prevention amount is invalid, 4 tons of cinnamaldehyde feed additive (100 mg/kg of cinnamaldehyde) produced in example 1 freely given by the Shandong Weifang Xinrong feed is added into the feed at the late 5 months and 7 days later, the market feedback has obvious effect after half a month, and particularly, the feed is used from 1 day old chicks, so that the feed is well managed, has no proventriculitis and obviously reduces the incidence of poor management. By the month of febrile and humid season proventriculitis began to worsen again, with some relief from addition to 600 g/t (cinnamaldehyde 150mg/kg), but poor management and barton conditions, especially in poorly ventilated chicken farms, were not used (1 kg/t was recommended) even with addition to 600 g/t (cinnamaldehyde 150mg/kg) (contact Qu teacher 135).

Example 19

159 x y x y x y x.

Symptoms are: proventriculitis occurs, 1.5kg of Jingluan-50B is continuously added into each ton of feed for 9 days, and all the feeds are recovered to be normal.

Example 20

One 156X Y H A V C B C A V C A V C A C B C A C B C A C B C A C B C A C B C A C B C A C B C A C B C A C B C A C B C.

Example 21

Shang guan Zhen of Shang Han county, Henan province, and Zi (Mobile phone 135X)

When 817 kinds of chickens are bred, proventriculitis begins to occur at 15 days, the symptom of anatomical proventriculitis can be clearly diagnosed, 1.5 kg/ton of the cinnamaldehyde feed additive produced in the example 1 is added into the feed, the feed is continuously used for 8 days, the whole chicken crowd normally feeds, and the symptom of anatomical proventriculitis is obviously slight.

Example 22

A species of a certain (cell phone 186 x) bred in guan tai town of Handan city, Hebei province: 817, the chickens grow unevenly, the ingestion is only about 60% of the normal level, the dissected glandular gastric ulcer is serious, 1.5 kg/ton of the cinnamaldehyde feed additive produced in the example 1 is added into the feed immediately, the feed is continuously used for 12 days, the whole chicken group ingests the chickens which are normal, and the dissected chickens are difficult to see the glandular gastritis symptoms again.

Example 23

The method comprises the following steps of (1) chatting in Shandong province city Gaotang county, Qing Ping Tou Liuhai village, breeding a certain Liu (cell phone 182. x.) with chicken breed: 817, uneven growth, listlessness, poor appetite, large swelling of the dissected glandular stomach, severe erosion of the muscular stomach, as shown in FIG. 14, for accurate diagnosis.

2 kg/ton of the cinnamaldehyde feed additive produced in the example 1 is added into the feed, the feed is continuously used for 12 days, the whole chicken flock is relatively good in alignment, the feeding is recovered to be normal, and only the chickens with the adenoid myogastritis symptoms cannot be seen through dissection.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. Application of cinnamaldehyde in preparing feed, feed additive, veterinary drug or water-drinking preparation for preventing or treating poultry adenoid myogastritis.

2. The use according to claim 1, wherein when the feed additive or veterinary drug is added to a feed, the concentration of cinnamaldehyde in the feed is 200-5000 mg/kg;

3. The use of claim 1, wherein when the feed additive, veterinary drug or drinking water preparation is used for preventing poultry adenoid gastritis and adenoid gastric erosion, the concentration of cinnamaldehyde added to the feed is 250-500 mg/kg, or the concentration of cinnamaldehyde in drinking water prepared from the drinking water preparation is 20-500 mg/kg, and the use method is as follows: the preparation is used for 5-12 days.

4. The use according to claim 3, characterized in that the method of use is: the medicine is used for 5-12 days, and is used once after 15-45 days, and the medicine is used for 3-25 days once.

5. The use according to claim 3, wherein the method of use is: the medicine is used for 5 to 12 days, and is used once after every 20 to 25 days, and the medicine is used for 3 to 5 days.

6. The use of claim 1, wherein when the feed additive, veterinary drug or drinking water preparation is used for treating poultry adenoid gastritis and adenoid gastric erosion, the concentration of cinnamaldehyde in the feed is 350-1250 mg/kg when the feed is mixed with the feed, or the concentration of cinnamaldehyde in drinking water prepared by the drinking water preparation is 75-625 mg/kg, and the use method is as follows: the preparation is used for 3-15 days.

7. The use according to claim 6, wherein the method of use is: the preparation is used for 3-15 days, and is used once after every 10-30 days, and the preparation is used for 3-15 days once.

8. The application of claim 1, wherein the poultry is laying poultry in an egg laying period, and when the feed additive or veterinary drug is mixed with a feed, the concentration of the cinnamaldehyde in the feed is 75-250 mg/kg, or the concentration of the cinnamaldehyde in drinking water prepared by a drinking water preparation is 37.5-125 mg/kg, the using time is 8-30 days/time, and the cinnamaldehyde is used once every 20-45 days and 8-15 days once to ensure the health of laying hens.

9. The use according to claim 1, wherein the feed, feed additive, veterinary drug or drinking water preparation further comprises an auxiliary component for preventing or treating poultry adenomyositis and adenomygastric erosion, and the auxiliary component comprises plant essential oil, Chinese herbal medicine or chemical composition.

10. Use according to claim 9, wherein the plant essential oil is selected from carvacrol, thymol or eugenol.