CN114642241B - Composite plant polyphenol chelate and application method thereof - Google Patents

Composite plant polyphenol chelate and application method thereof Download PDF

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CN114642241B
CN114642241B CN202210347360.3A CN202210347360A CN114642241B CN 114642241 B CN114642241 B CN 114642241B CN 202210347360 A CN202210347360 A CN 202210347360A CN 114642241 B CN114642241 B CN 114642241B
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chelate
baicalin
plant polyphenol
intestinal
puerarin
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CN114642241A (en
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丁斌鹰
侯永清
张正帆
郭双双
吴涛
李中华
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Wuhan Polytechnic University
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/116—Heterocyclic compounds
    • A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry

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  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
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Abstract

The invention discloses a compound plant polyphenol chelate and a use method thereof, which belong to the technical field of animal husbandry, and comprise baicalin copper, 4' -butyryl puerarin and zinc tannate, wherein the mass ratio of the raw materials is 1: 3-5: 4-6. The invention can effectively inhibit intestinal bacteria and viruses, enhance the oxidation resistance and the immunity of the intestinal canal, promote the development of intestinal mucosa, thereby improving the intestinal canal function of livestock and poultry; the compound plant polyphenol chelate can reduce the incidence rate of intestinal diseases, promote the health of livestock and poultry and improve the production performance; compared with veterinary drug additives for feeding, the additive has the advantages of no toxic or side effect, no drug residue, no pollution and being beneficial to animal and human health.

Description

Composite plant polyphenol chelate and application method thereof
Technical Field
The invention relates to the technical field of animal husbandry, in particular to a compound plant polyphenol chelate and a use method thereof.
Background
Plant polyphenols are a class of secondary metabolites with a polyphenol structure that are widely present in plants, and are bioflavonoids with a specific molecular structure. The plant polyphenol is natural antioxidant, has effects of scavenging free radicals, resisting oxidation, resisting aging, resisting allergy, and inflammation, enhancing immunity, resisting tumor, and preventing cancer, and has protecting effects on heart and cerebral vessels. Such as baicalin has antibacterial, diuretic, antiinflammatory, cholesterol reducing, thrombosis resisting, asthma relieving, pathogenic fire purging, toxic substances removing, hemostatic, miscarriage preventing, antiallergic, and spasmolytic effects; tannic acid has been used in medicine for treating laryngopharyngitis, tonsillitis, hemorrhoid, skin herpes, etc., and can be used for preventing diarrhea and intestinal hemorrhage by internal use; puerarin has antipyretic, sedative and coronary blood flow increasing effects, and can be used for protecting acute myocardial hemorrhage caused by posterior pituitrin. However, most plant polyphenols do not perform well in humans and animals due to their low solubility in esters, water, or low ester/water numbers. In order to better exert the bioactive effect of plant polyphenols in protecting human and animal intestinal health, they are generally derived according to their physicochemical properties, in order that these substances are better soluble in water or esters. The animal intestinal canal is a water-soluble system, so that the baicalin copper, the tannic acid zinc and the 4' -butyric acid puerarin are found to have the characteristic of being easily dissolved in water in the existing baicalin, tannic acid and puerarin derivatives, and have better antioxidation property compared with the original plant polyphenol. The substances with the antioxidant function are added into animal feed or drinking water, so that the intestinal functions of livestock and poultry can be improved, the growth performance and the health level of the livestock and poultry can be promoted, the use of growth promoters and therapeutic drugs can be reduced, and a foundation is laid for the healthy and sustainable development of the breeding industry.
The intestinal tract of livestock and poultry is not only the largest digestive absorption organ in the animal body, but also the largest immune organ in the body. The development status and the functions of the intestinal canal are important to the growth and health of livestock and poultry. The intestinal tracts of young livestock and poultry are in rapid development, have imperfect structure and function, and have great limitations on digestion and absorption of nutrients and immune barrier function. In addition, various stresses, bacteria and viruses can promote the intestinal tracts of livestock and poultry to generate excessive oxygen free radicals, so that intestinal mucosa is atrophic, and intestinal epithelium integrity and mucosa barrier are destroyed, thereby causing intestinal dysfunction. Therefore, effective measures are urgently needed to be taken in livestock and poultry breeding production to improve the intestinal development condition of livestock and poultry, maintain and improve the intestinal function, and therefore, we propose a compound plant polyphenol chelate and a use method thereof.
Disclosure of Invention
The invention aims to provide a compound plant polyphenol chelate. The compound plant polyphenol chelate can effectively inhibit enterobacteria and viruses, enhance the oxidation resistance and the immune function of the intestinal tract, promote the development of intestinal mucosa, and further improve the intestinal function of livestock and poultry. The compound plant polyphenol chelate can reduce the incidence rate of intestinal diseases, promote the health of livestock and poultry and improve the production performance, and has the advantages of no toxic or side effect, no drug residue, no pollution and being beneficial to the health of animals and human beings compared with a veterinary drug additive for feeding.
Another object of the present invention is to provide a method of using the complex plant polyphenol chelate.
In order to solve the problems, the invention adopts the following technical scheme:
a compound plant polyphenol chelate comprises baicalin copper, 4' -butyryl puerarin and zinc tannate, wherein the mass ratio of the raw materials is 1: 3-5: 4-6.
As a preferable scheme of the invention, the baicalin copper is a product prepared from baicalin-7-O-beta-D-glucuronide and copper chloride in methanol medium, and the baicalin content is at least 75% and the copper content is 8.5-9.0%.
As a preferable scheme of the invention, the preparation method of the baicalin copper comprises the following steps: baicalin-7-O-beta-D-glucuronide is obtained from baicalein, baicalin-7-O-beta-D-glucuronide and copper chloride are reacted in methanol solution at 50 ℃ for 3 hours according to the mass ratio of 2:1 to obtain sediment, and the filter residue is dried at low temperature to obtain baicalin copper.
As a preferable scheme of the invention, the 4 '-butyric acid acyl puerarin is a product prepared from 8-beta-D-glucopyranose-4', 7-dihydroxyisoflavone and butyric acid acyl chloride in acetone and sodium hydroxide medium, and the puerarin content is at least 85% and the butyric acid content is 7.8-8.2%.
As a preferable scheme of the invention, the preparation method of the 4' -butyryl puerarin comprises the following steps: 8-beta-D-glucopyranose-4', 7-dihydroxyisoflavone is prepared from wild kudzuvine root according to the mass ratio of 1.5:1 weighing 8-beta-D-glucopyranose-4 ', 7-dihydroxyisoflavone and butyric acid acyl chloride, respectively dissolving in saturated sodium hydroxide solution and acetone solution with equal volume, uniformly mixing the two solutions, reacting at 20 ℃ for 2.5h, separating and filtering to obtain 4' -butyric acid puerarin.
As a preferable scheme of the invention, the product of the zinc tannate prepared from tannic acid and zinc chloride in methanol medium has the tannic acid content of at least 80% and the zinc content of 6.0-7.0%.
As a preferable scheme of the invention, the preparation method of the zinc tannate comprises the following steps: tannic acid is obtained from gallnut, and tannic acid and zinc chloride are mixed according to the mass ratio of 1:1.5 reacting in 45 ℃ ethanol solution for 2 hours, and obtaining zinc tannate through spray drying.
The application method of the compound plant polyphenol chelate comprises the steps of adding the compound plant polyphenol chelate into livestock and poultry feed or drinking water according to the weight of 0.01-0.05%; mixing the compound plant polyphenol chelate with water to directly drink water for use; and mixing the compound plant polyphenol chelate with a carrier to prepare a premix, or mixing the compound plant polyphenol chelate with a feed additive and a feed raw material to prepare a premix and a concentrate for feeding livestock and poultry.
Compared with the prior art, the invention has the advantages that:
the scheme can effectively inhibit intestinal bacteria and viruses, enhance the oxidation resistance and the immunity of the intestinal tracts, promote the development of intestinal mucosa, and further improve the intestinal functions of livestock and poultry; the compound plant polyphenol chelate can reduce the incidence rate of intestinal diseases, promote the health of livestock and poultry and improve the production performance; compared with veterinary drug additives for feeding, the additive has the advantages of no toxic or side effect, no drug residue, no pollution and being beneficial to animal and human health.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in connection with the embodiments of the present invention. It is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments, and that all other embodiments obtained by persons of ordinary skill in the art without making creative efforts based on the embodiments in the present invention are within the protection scope of the present invention.
A compound plant polyphenol chelate comprises baicalin copper, 4' -butyryl puerarin and zinc tannate, wherein the mass ratio of the raw materials is 1: 3-5: 4-6; wherein, the baicalin copper is a product prepared from baicalein-7-O-beta-D-glucuronide and copper chloride in methanol medium, and the baicalein content is at least 75 percent, and the copper content is 8.5-9.0 percent; the puerarin 4 '-butyrate is a product prepared from 8-beta-D-glucopyranose-4', 7-dihydroxyisoflavone and butyric acid acyl chloride in acetone and sodium hydroxide medium, and the puerarin content is at least 85% and the butyric acid content is 7.8-8.2%; the zinc tannate is a product prepared from tannic acid and zinc chloride in methanol medium, wherein the tannic acid content is at least 80%, and the zinc content is 6.0-7.0%.
The preparation method of the baicalin copper comprises the following steps: baicalin-7-O-beta-D-glucuronide is obtained from baicalein, baicalin-7-O-beta-D-glucuronide and copper chloride are reacted in methanol solution at 50 ℃ for 3 hours according to the mass ratio of 2:1 to obtain sediment, and the filter residue is dried at low temperature to obtain baicalin copper;
the preparation method of the 4' -butyryl puerarin comprises the following steps: 8-beta-D-glucopyranose-4', 7-dihydroxyisoflavone is prepared from wild kudzuvine root according to the mass ratio of 1.5:1, weighing 8-beta-D-glucopyranose-4 ', 7-dihydroxyisoflavone and butyric acid acyl chloride, respectively dissolving in saturated sodium hydroxide solution and acetone solution with equal volume, uniformly mixing the two solutions, reacting for 2.5 hours at 20 ℃, separating and filtering to obtain 4' -butyric acid puerarin;
the preparation method of the zinc tannate comprises the following steps: tannic acid is obtained from gallnut, and tannic acid and zinc chloride are mixed according to the mass ratio of 1:1.5 reacting in 45 ℃ ethanol solution for 2 hours, and obtaining zinc tannate through spray drying.
Example 1:
the mass ratio of the baicalin copper to the 4' -butyryl puerarin to the zinc tannate is 1:4: and 5, combining, and uniformly mixing the compounds to obtain the compound plant polyphenol chelate.
The compound plant polyphenol chelate is suitable for piglets in a drinking water drenching mode, and has obvious application effect.
The compound plant polyphenol chelate of the example is used for feeding piglets in a drinking water way. 24 piglets with age of 2.1+/-0.5 kg of 7 days are selected and divided into 3 groups, no medicine or health care agent is added in drinking water of a blank control group, the doxycycline and amoxicillin mixed agent is infused into the drinking water of a test group I, the doxycycline and amoxicillin mixed agent is 0.5g/L, the compound plant polyphenol chelate is 0.5g/L in the drinking water of a test group II, and the weight, diarrhea incidence and diarrhea index of the piglets are counted after the 21-day-age test of the piglets is finished. The results are shown in Table 1.
TABLE 1 influence of the addition of the complex plant polyphenol chelate to the drinking water on piglets
Project Blank control group Test group I Test group II
21-day-old body weight (kg/man) 5.19 b ±0.85 5.67 a ±0.64 5.75 a ±0.47
Incidence of diarrhea (%) 23.27 a ±9.93 1.68 b ±0.52 1.72 b ±0.57
Diarrhea index 1.35 a ±1.70 0.35 b ±0.22 0.32 b ±0.19
Note that: the same row of shoulder letters indicates significant differences (p < 0.05). The following is similar.
The results show that compared with a blank control group, the feed additive provided by the invention can obviously improve the weight of piglets by 10.79% at 21 days, and reduce the diarrhea incidence rate by 92.61% and the diarrhea index by 74.07%. Compared with the medicine drinking water group, the difference of various observation indexes is not obvious. The results show that the compound plant polyphenol chelate is used for feeding piglets in a drinking water mode, and the compound plant polyphenol chelate has the advantages of replacing medicine drinking water to ensure the growth performance of the piglets.
Example 2:
the mass ratio of the baicalin copper to the 4' -butyryl puerarin to the zinc tannate is 1:5: and 4, combining, and uniformly mixing the compounds to obtain the compound plant polyphenol chelate.
The compound plant polyphenol chelate is suitable for laying hens in a drinking manner, and has obvious application effect.
The compound plant polyphenol chelate compound of the example is used for feeding the laying hens with enteritis in a drinking manner. 300 sea-blue brown laying hens of the same day age, which are infected with mycoplasma and bacillus to cause diarrhea, are selected to be divided into 3 groups, the control group does not receive any treatment, the test I group receives 0.5g/L of tylosin tartrate through drinking water for 5 days, the test II group receives 0.2g/L of the compound plant polyphenol chelate through drinking water for 5 days, and the production performance, the diarrhea incidence and the intestinal score are counted after the test is finished. The results are shown in Table 2.
TABLE 2 influence of addition of the present complex plant polyphenol chelates to layer eggs in drinking water
Project Control group Test group I Test group II
Feed intake (g/d/only) 82.06 a ±2.26 112.13 b ±1.72 111.16 b ±2.41
Laying rate (%) 37.89 c ±7.08 76.3 b ±6.48 78.05 a ±5.74
Incidence of diarrhea (%) 91.23 c ±0.39 5.68 b ±0.52 4.80 a ±0.58
Intestinal scoring 4.53 a ±1.07 2.47 b ±1.05 2.48 b ±0.98
The results show that compared with a control group which is not treated, the feed additive provided by the invention can obviously improve the feed intake of the laying hens by 35.46% and the laying rate by 51.45%, reduce the diarrhea rate by 51.6% and reduce the intestinal score by 45.25%. Compared with the drinking water medicine treatment group, the method has the advantages of remarkably improving the laying rate, reducing the diarrhea incidence rate, and having no remarkable difference in feed intake and intestinal score. The results show that the compound plant polyphenol chelate is used for feeding the laying hen in a drinking water manner, so that the egg laying performance and feed intake of the laying hen are guaranteed to be equivalent to the effect of the medicine, and the effect of relieving diarrhea incidence and intestinal scores of the laying hen suffering from enteritis is better than the effect of the medicine.
Example 3:
the mass ratio of the baicalin copper to the 4' -butyryl puerarin to the zinc tannate is 1:3:6, combining, and uniformly mixing the compounds to obtain the compound plant polyphenol chelate.
The compound plant polyphenol chelate is suitable for the suckling piglets and has obvious application effect.
The compound plant polyphenol chelate of the example is used for feeding weaned pigs. The 72 10 day old piglets were divided into three groups, three replicates (columns) of each group, 8 heads per column. The source, the age of the piglets and the weight of the piglets are close, and the sexes are consistent. The basic ration is fed every day, wherein the blank control group is not added with the compound plant polyphenol chelate and the florfenicol premix, 100mg/kg of florfenicol is added in the test group I, and 0.01% of the compound plant polyphenol chelate is added in the test group II. Raising for 21 days. The results are shown in tables 3, 4, 5 and 6.
TABLE 3 influence of addition of Compound plant polyphenol chelate on growth performance of weaned pigs
Project Blank control group Test group I Test group II
Average daily gain ADG (g) 103.33 b ±10.21 115.00 ab ±10.00 119.67±7.55 a
Average daily feed intake ADFI (g) 200.57±7.47 210.60±10.77 215.60±7.93
Ratio of weight to weight F/G 1.94 a ±0.19 1.83 b ±0.42 1.80 b ±0.13
Incidence of diarrhea (%) 7.60 a ±0.02 3.50 b ±0.04 3.15 b ±0.04
TABLE 4 influence of addition of Compound plant polyphenol chelate on the number of cells related to jejunal mucosa immunity of weaned pigs
Project Blank control group Test group I Test group II
Lymphocyte (individual/100 IEL) 53.5 c ±1.6 60.2 b ±0.9 65.2 a ±1.2
Mast cell (individual/intestinal noodle) 1625 b ±391 1899 b ±331 2118 a ±433
Goblet cell (personal/100 IEL) 4.6 c ±0.3 5.1 b ±0.4 6.1 a ±0.4
TABLE 5 influence of addition of complex plant polyphenol chelate on the villus height/crypt depth of small intestine of weaned pigs
Project Blank control group Test on testGroup I test Test group II
Duodenum 1.12 c ±0.02 1.23 b ±0.08 1.36 a ±0.07
Jejunum 1.01 c ± 0.09 1.12 b ±0.04 1.27 a ±0.12
Ileum of the body 1.38 c ±0.10 1.51 b ±0.09 1.65 a ±0.06
TABLE 6 influence of addition of Compound plant polyphenol chelate on antioxidant capacity of intestinal mucosa of weaned pigs
Project Blank control group Test group I Test group II
MDA (nmol/mg protein) 1.07 a ± 0.12 0.79 b ± 0.20 0.81 b ± 0.11
SOD (U/mg protein) 43.28 c ± 3.18 49.27 b ± 1.93 53.73 a ± 2.06
CAT (U/mg protein) 23.85 c ± 2.72 26.63 b ± 2.19 31.16 a ± 1.68
GSH (mg/g protein) 21.76 ± 1.54 23.11 ± 1.08 22.45 ± 1.89
The data in tables 3-6 show that when the composite plant polyphenol chelate disclosed by the invention is applied, compared with a blank control group, the daily gain of piglets is improved by 15.81%, the feed weight ratio is reduced by 7.22%, and the diarrhea rate is reduced by 59.21% by adding the composite plant polyphenol chelate in the feed; the numbers of lymphocytes, mast cells and goblet cells in jejunum cells increased by 21.87%, 30.34% and 32.61%, respectively; the ratio of villus height to crypt depth of duodenum, jejunum and ileum increased by 21.43%, 25.74% and 19.56%, respectively; the content of Malondialdehyde (MDA) in intestinal mucosa is reduced by 24.30%, and the activity of superoxide dismutase (SOD) is improved by 24.15% and the activity of Catalase (CAT) is improved by 30.64%; compared with a drug group, the compound plant polyphenol chelate is added into the feed, so that the daily gain of piglets is increased by 4.06%; the material weight ratio is reduced by 1.64%; the diarrhea rate is reduced by 10%; the numbers of lymphocytes, mast cells and goblet cells in jejunum cells were increased by 8.30%, 11.53% and 19.60%, respectively; the ratio of the villus height to the crypt depth of the duodenum, jejunum and ileum is increased by 10.57%, 13.40% and 9.27%, respectively; the content of Malondialdehyde (MDA) in intestinal mucosa is not different, and the activities of super SOD and CAT are respectively improved by 9.05 percent and 17.01 percent.
In a word, the results show that the compound plant polyphenol chelate has the effects of improving the small intestinal mucosa structure of piglets, improving the oxidation resistance of intestinal tracts, and improving the number of intestinal mucosa immune cells, and is superior to antibiotics, so that the compound plant polyphenol chelate can replace antibiotics to promote the intestinal immunity and oxidation resistance in piglet feeds, thereby improving the productivity of piglets.
Example 4:
the mass ratio of the baicalin copper to the 4' -butyryl puerarin to the zinc tannate is 1:5: and 4, combining, and uniformly mixing the compounds to obtain the compound plant polyphenol chelate.
Meanwhile, firstly, 1 part of 75% baicalin is dissolved in 1 part of copper chloride aqueous solution containing 8.5% of copper, and the mixture is obtained by drying at 65 ℃; secondly, 1 part of 85% puerarin and 1 part of 8% butyric acid aqueous solution are dried at 65 ℃ to obtain a second mixture; then 1 part of 80% tannic acid and 1 part of aqueous solution containing 6% zinc chloride are dried at 65 ℃ to obtain a third mixture; finally, three compounds are mixed according to the mass ratio of 1:5:4 to obtain a mixture (hereinafter referred to as a mixture) with the same composition as the compound plant polyphenol chelate.
The compound plant polyphenol chelate of this example was used for broiler feeding experiments. 817 broilers 360 hatched in the same batch are divided into 3 groups of 8 replicates (columns) each, and 15 chickens each. The nutrition level of three groups of basic diets is consistent, wherein the aflatoxin content in the feed is 500 mug/kg, the blank control group is not added with the compound plant polyphenol chelate and the compound of the invention, the test I group is added with 0.04 percent of the compound plant polyphenol chelate consistent with the compound plant polyphenol chelate composition of the example, and the test II group is added with 0.04 percent of the compound plant polyphenol chelate of the example. Raising for 35 days. The results are shown in tables 7, 8 and 9.
TABLE 7 influence of addition of complex plant polyphenol chelates on broiler growth performance
Project Blank control group Test group I Test group II
Daily gain ADG (g/d) 16.7 c ±0.12 18.3 b ±0.17 20.1 a ±0.09
Daily feed intake ADFI (g/d) 32.6 c ±0.2 35.2 b ±0.1 37.8 a ±0.1
Ratio of weight to weight F/G 1.95 a ±0.02 1.92 a ±0.01 1.88 b ±0.02
TABLE 8 influence of addition of Compound plant polyphenol chelate on antioxidant capacity and SlgA expression level of intestinal empty field mucosa of broiler chickens
Project Blank control group Test group I Test group II
MDA (nmol/mg protein) 3.87 a ±0.66 2.82 b ±0.71 1.96 c ±0.64
CAT (U/g protein) 2.15 c ±0.13 3.03 b ±0.20 a 4.10 a ±0.26
Area of expression of SlgA duodenum μm 2 4029.21 c ±335.61 4518.12 b ±193.15 5372.94 a ±167.45
TABLE 9 influence of addition of complex plant polyphenol chelates on the small intestine villus height/crypt depth of broilers
Project Blank control group Test group I Test group II
Duodenum 1.09 b ±0.17 1.21 b ±0.09 1.58 a ±0.14
Jejunum 1.18 b ±0.25 1.31 b ±0.25 1.63 a ±0.33
The result shows that when the ration contains 500 mug/kg aflatoxin, the daily gain and the feed intake of the broiler chickens are respectively improved by 20.35 percent and 15.95 percent and the feed weight ratio is reduced by 3.59 percent by applying the composite plant polyphenol chelate disclosed by the invention compared with a blank control group; the ratio of the villus height to the crypt depth of the duodenum and jejunum ileum is increased by 44.95% and 38.14%, respectively; the content of Malondialdehyde (MDA) in jejunum mucous membrane is reduced by 49.35%, and the super CAT activity 90.07% and the mucous membrane SlgA expression level are improved by 33.35%. Compared with a test II group fed with a mixture with the same composition as the compound plant polyphenol chelate compound of the example, the daily gain and the feed intake of the broiler chickens are respectively improved by 9.52 percent and 7.38 percent, and the feed weight ratio is reduced by 2.08 percent; the ratio of the villus height to the crypt depth of the duodenum and jejunum ileum is respectively improved by 30.57 percent and 24.43 percent; the content of Malondialdehyde (MDA) in jejunum mucous membrane is reduced by 30.49%, and the super CAT activity is improved by 35.31% and the mucous membrane SlgA expression quantity is improved by 18.92%.
In conclusion, the results show that the compound plant polyphenol chelate has the advantages of improving the small intestine mucous membrane structure of the broiler chickens, improving the oxidation resistance of the intestinal tracts and increasing the SIgA expression quantity of the intestinal mucous membranes, so that the production performance of the broiler chickens is improved, and the effect is better than that of a mixture with the same composition as that of the compound plant polyphenol chelate.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical solution and the modified concept thereof, within the scope of the present invention.

Claims (1)

1. The compound plant polyphenol chelate is characterized by comprising baicalin copper, 4' -butyryl puerarin and zinc tannate, wherein the mass ratio of the raw materials is 1:3 to 5: 4-6, wherein the preparation method of the baicalin copper comprises the following steps: baicalin-7-O-beta-D-glucuronide is obtained from baical skullcap root, baicalin-7-O-beta-D-glucuronide and cupric chloride are reacted in methanol solution at 50 ℃ for 3 hours according to the mass ratio of 2:1 to obtain precipitate, filter residues are dried at low temperature to obtain baicalin copper, and the baicalin content is at least 75 percent, and the copper content is 8.5-9.0 percent; the preparation method of the 4' -butyryl puerarin comprises the following steps: 8-beta-D-glucopyranose-4', 7-dihydroxyisoflavone is prepared from wild kudzuvine root according to the mass ratio of 1.5:1, weighing 8-beta-D-glucopyranose-4 ', 7-dihydroxyisoflavone and butyric acid acyl chloride, respectively dissolving in saturated sodium hydroxide solution and acetone solution with equal volume, uniformly mixing the two solutions, reacting for 2.5 hours at 20 ℃, separating and filtering to obtain 4' -butyric acid puerarin, wherein the puerarin content is at least 85% and the butyric acid content is 7.8-8.2%; the preparation method of the zinc tannate comprises the following steps: tannic acid is obtained from gallnut, and tannic acid and zinc chloride are mixed according to the mass ratio of 1:1.5 reacting in 45 ℃ ethanol solution for 2 hours, and obtaining zinc tannate through spray drying.
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