CN116549534B - Pharmaceutical composition for promoting growth of egg chicks and regulating intestinal functions - Google Patents
Pharmaceutical composition for promoting growth of egg chicks and regulating intestinal functions Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A61K31/00—Medicinal preparations containing organic active ingredients
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
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Abstract
The invention discloses a pharmaceutical composition for promoting the growth of egg chicks and regulating intestinal functions, which consists of 1 g-2 g/kg of medlar and 0.4g/kg of mannooligosaccharide. The composition selects the optimal combination proportion according to physiological characteristics of different growth stages of the egg chicks, promotes the growth of the chicks, and improves intestinal digestion function and mucous membrane barrier function of the chicks. In vivo experiments show that: the combination of the two can enhance the growth performance of the egg chicks, enhance the digestion function of the chicks by enhancing the intestinal digestive enzyme activity, enhance the villus height and the villus cryptoratio of the small intestine, enhance the intestinal barrier function by up-regulating the relative expression quantity of intestinal tissue tight junction protein ZO-1, mucin MUC2 and atresia Occludin mRNA, and regulate the intestinal health state by reducing the pH of the cecum and increasing the number of lactobacillus in the cecum content.
Description
Technical Field
The invention relates to the technical field of Chinese herbal medicines and the technical field of poultry anti-pathogen cultivation, in particular to a pharmaceutical composition for promoting the growth of egg chickens and regulating intestinal functions.
Background
Due to excessive and excessive use of antibiotics, bacterial drug resistance is generated, the clinical treatment effect of veterinary antibiotics is reduced, the dosage of antibiotics is forced to be increased in the breeding industry, the residual risk of veterinary antibacterial drugs is increased, and the quality safety of livestock and poultry products is threatened.
The wolfberry fruits are rich in rich alkaloid, polysaccharide, flavonoid, fenic acid and other components, have the effects of resisting inflammation, regulating immunity, promoting growth, enhancing intestinal barrier and the like, and according to statistics, the annual planting area of the wolfberry fruits in Ningxia county exceeds 20 mu, the annual yield of dried wolfberry fruits exceeds 40000 tons, and the leftovers (mainly inferior fruits: too small fruits, broken fruits and pomace) generated in the wolfberry processing process reach over 2000 tons.
The mannooligosaccharide has been widely studied in human and animal health care, and has the effects of promoting growth, improving immunity and regulating the environment in intestinal tract, and has the advantages of less drug resistance and less drug residue.
However, the effect of single use of traditional Chinese medicine or mannooligosaccharide may have a certain limitation, so that a high-efficiency combination scheme is sought, and therefore, the screening of effective medicines in traditional Chinese herbal medicines and the combination of mannooligosaccharide are used for replacing growth-promoting antibiotics, thereby completely meeting the development requirements of healthy cultivation in modern society.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a pharmaceutical composition for promoting the growth of the egg chicks and regulating the intestinal functions, and the pharmaceutical composition combines medlar fruit powder and mannooligosaccharide, so that the growth performance of the egg chicks is effectively improved, and the intestinal functions are enhanced.
The technical scheme adopted for achieving the aim of the invention is as follows: a pharmaceutical composition for promoting the growth of egg chicks and regulating intestinal functions comprises 1 g-2 g/kg of medlar and 0.4g/kg of mannooligosaccharide.
The optimal proportion of the pharmaceutical composition for promoting the growth of the egg chicks and regulating the intestinal functions is composed of 2g/kg of medlar and 0.4g/kg of mannooligosaccharide.
The invention relates to a pharmaceutical composition for promoting the growth of egg chickens and regulating intestinal functions, wherein medlar is medlar powder.
The invention relates to a pharmaceutical composition for promoting the growth of egg chickens and regulating intestinal functions, wherein the egg chickens are 7-49 days old.
It is still another object of the present invention to provide a pharmaceutical composition for promoting the growth of egg chickens and regulating intestinal function for the treatment of intestinal digestive function and intestinal mucosal barrier function.
The specific application of the pharmaceutical composition for promoting the growth of the egg chicks and regulating the intestinal functions in different growth stages of the egg chicks is as follows:
s1, in the daily ration of the chicks 7, starting to use 2g/kg, 1g/kg and 0.5g/kg of medlar residual fruit powder and above doses respectively with 0.4g/kg of mannooligosaccharide and 0.4g/kg of mannooligosaccharide in the daily ration, and calculating the average daily gain and the feed-weight ratio of the chicks per week.
S2, under the same treatment as the S1, determining the duodenal digestive enzyme activity of the chicks at 28 days old and 49 days old respectively, and determining the villus height, the crypt depth and the villus-crypt ratio of the small intestine, thereby determining the relative expression quantity of the protein mRNA related to the mucous membrane barrier of the small intestine.
According to the application method, the chicks are divided into different growth stages according to daily ration, and proper combination proportion is selected according to physiological characteristics of each growth stage and the existing research results, so that the medication science and safety are improved, and meanwhile, the production investment is reduced.
Drawings
FIG. 1 is a graph showing the effect of adding 2g/kg of wolfberry to the diet on the activity of the duodenal digestive enzyme of a chick;
FIG. 2 is a graph showing the effect of the combination of 2g/kg of wolfberry and 0.4g/kg of mannooligosaccharide on the activity of the duodenal digestive enzyme of a chick;
FIG. 3 is a graph showing the effect of adding 1g/kg of wolfberry and 0.4g/kg of mannooligosaccharide to the chick duodenal digestive enzyme activity;
FIG. 4 is a graph showing the effect of high doses of Lycium barbarum and mannooligosaccharide on the relative expression levels of chicken intestine Occlude;
FIG. 5 is a graph showing the effect of high doses of Lycium barbarum and mannooligosaccharide on the relative expression level of ZO-1mRNA in the small intestine of chickens;
FIG. 6 is a graph showing the effect of the dose of Lycium barbarum and mannooligosaccharide on the relative expression level of chicken intestine Occlude;
FIG. 7 is a graph showing the effect of the dose of Lycium barbarum and mannooligosaccharide on the relative expression level of ZO-1mRNA in the small intestine of chicks;
FIG. 8 is a graph showing the effect of low dose matrimony vine and mannooligosaccharide on the relative expression level of chick small intestine Occludin;
FIG. 9 is a graph showing the effect of low dose of Lycium barbarum and mannooligosaccharide on the relative expression level of ZO-1mRNA in the small intestine of chicks;
FIG. 10 is a graph showing the effect of high doses of Lycium barbarum and mannooligosaccharide on the relative expression level of MUC2 mRNA in the intestinal tract of chickens;
FIG. 11 is a graph showing the effect of dose of matrimony vine and mannooligosaccharide on the relative expression level of MUC2 mRNA in the intestinal tract of chickens;
FIG. 12 is a graph showing the effect of low doses of matrimony vine and mannooligosaccharide on the relative expression level of MUC2 mRNA in the intestinal tract of chickens;
FIG. 13 is a graph showing the effect of matrimony vine and mannooligosaccharide on the number of E.coli in chickens;
FIG. 14 is a graph showing the effect of matrimony vine and mannooligosaccharide on the number of Lactobacillus cecum in chickens.
Detailed Description
Hereinafter, only certain exemplary embodiments are briefly described. As will be recognized by those skilled in the pertinent art, the described embodiments may be modified in numerous different ways without departing from the spirit or scope of the embodiments of the present invention, which should also be considered as being within the scope of the present invention. Accordingly, the description of the embodiments is to be regarded as illustrative in nature and not as restrictive.
Example 1
A pharmaceutical composition for promoting growth of egg chicken and regulating intestinal function comprises 1g/kg fructus Lycii and 0.4g/kg mannooligosaccharide.
Example 2
A pharmaceutical composition for promoting growth of egg chicken and regulating intestinal function comprises fructus Lycii 2g/kg and mannooligosaccharide 0.4 g/kg.
Test example 1: testing of growth performance of egg chicks
Chicks are randomly divided into 8 groups, 3 level repeats are set, each repeat is carried out on 12 chicks, 2g/kg, 1g/kg, 0.5g/kg of medlar residue fruit powder and above in daily ration are respectively combined with 0.4g/kg of mannooligosaccharide and 0.4g/kg of mannooligosaccharide in daily ration 7 daily ration, and specific groups are shown in table 1. The test period was 42 days. In the raising process, the raising conditions of each group of chicks are consistent, the chicks can eat and drink water freely, and the chicken house can be subjected to normal disinfection and immunization procedures.
TABLE 1 chick grouping
| Grouping | Treatment of |
| Blank group (CON) | Basic daily ration for feeding |
| High-dose group of defective medlar fruit (LBH) | Basic ration +2g/kg of medlar residual fruit |
| Chinese wolfberry incomplete fruit dose group (LBM) | Basic ration +1g/kg of medlar residual fruit |
| Chinese wolfberry fruit low dose group (LBL) | Basic ration plus 0.5g/kg of medlar residual fruit |
| High dose combination (LBH+ACT) | Basic ration, 2g/kg of medlar residual fruit and 0.4g/kg of mannooligosaccharide |
| Medium dose combination (LBM+ACT) | Basic ration, 1g/kg of medlar residual fruit and 0.4g/kg of mannooligosaccharide |
| Low dose combination (LBL+ACT) | Basic ration, 0.5g/kg of medlar residual fruit, 0.4g/kg of mannooligosaccharide |
| Mannooligosaccharide group (ACT) | Basic ration plus 0.4g/kg mannooligosaccharide |
Note that: the control group, the high, medium, low dose combination group, and the mannooligosaccharide group are denoted by CON, LBH, LBM, LNL, LBH +act, lbm+act, lbl+act, and ACT, respectively, and are the same in the following tables and figures.
The weekly feed consumption of the test chicks was recorded during the test, the weight was weighed on an empty stomach before feeding every morning and recorded, and the Average Daily Gain (ADG) and the feed-to-weight ratio (F/G) of the chicks per week were calculated.
As shown in Table 2, when 2g/kg of fructus Lycii residue fruit powder and 0.4g/kg of mannooligosaccharide are mixed for feeding, average daily weight gain of chicks at 3, 4, 6 and 7 weeks can be improved, and the feed-weight ratio of 6 and 7 weeks can be reduced. Showing that the chick growth performance can be improved when 2g/kg of medlar residual fruit powder and 0.4g/kg of mannooligosaccharide are mixed for feeding.
TABLE 2 Effect of Lycium barbarum and mannooligosaccharide on chick growth Performance
Note that: the same lower case letters of the shoulder marks are compared with each other, two upper case letters indicate that the difference is extremely remarkable (P < 0.01), and one upper case letter indicates that the difference is remarkable (P < 0.05).
Test example 2: intestinal digestion function determination test for egg chicks
The above test example 1 group feeding scheme was carried out by dissecting chicks at 28 and 49 days of age, respectively, taking the duodenal contents, carrying out trypsin, lipase and amylase activity detection by using an enzyme activity test kit, taking the duodenum, jejunum and ileum sections, washing cleanly, placing in 4% paraformaldehyde, fully fixing to prepare paraffin sections, then HE staining, measuring the villus height and crypt depth of the small intestine, and calculating the villus-crypt ratio.
Fig. 1 to 3 are graphs showing the effect of various proportions of wolfberry and mannooligosaccharides on the activity of the chicken duodenal digestive enzyme. And (3) injection: * Indicating significant differences (P < 0.05), indicating very significant differences (P < 0.01).
As shown in figures 1-3, 2g/kg of medlar residual fruit powder and 2g/kg and 1g/kg of medlar residual fruit powder are added into daily ration, and when combined with 0.4g/kg of mannooligosaccharide, trypsin and lipase activities of 28-day old chickens and 49-day old chickens can be obviously improved; the combination of the three components can obviously improve the activity of 28-day-old duodenal amylase of chicks, and the combination effect of 2g/kg of medlar residual fruit powder and 0.4g/kg of mannooligosaccharide is the best.
2G/kg of medlar residual fruit powder and 2g/kg of medlar residual fruit powder are added into daily ration, and are respectively combined with 0.4g/kg of mannooligosaccharide, so that the jejunum villus height of 28-day-old chicks can be obviously increased; 1g/kg of medlar residual fruit powder is added, and the combination of the medlar residual fruit powder and 0.4g/kg of mannooligosaccharide can obviously improve jejunum villus height of 49-day-old chicks; the combination of 2g/kg and 1g/kg of medlar residual fruit powder and 0.4g/kg of mannooligosaccharide can obviously improve the villus height and the villus-to-crypto ratio of 28-day-old and 49-day-old ileum of chicks, as shown in tables 3, 4 and 5.
TABLE 3 influence of defective Lycium barbarum and mannooligosaccharide on the morphological structure of the duodenum of chicks
Note that: the same lower case letters of the shoulder marks are compared with each other, two upper case letters indicate that the difference is extremely remarkable (P < 0.01), and one upper case letter indicates that the difference is remarkable (P < 0.05).
TABLE 4 Effect of Lycium barbarum and mannooligosaccharide on the morphology and Structure of the jejunum of chicks
Note that: the same lower case letters of the shoulder marks are compared with each other, two upper case letters indicate that the difference is extremely remarkable (P < 0.01), and one upper case letter indicates that the difference is remarkable (P < 0.05).
TABLE 5 Effect of Lycium barbarum and mannooligosaccharide on ileum morphology in chicks
Note that: the same lower case letters of the shoulder marks are compared with each other, two upper case letters indicate that the difference is extremely remarkable (P < 0.01), and one upper case letter indicates that the difference is remarkable (P < 0.05).
It is proved that when 2g/kg and 1g/kg of medlar residual fruit powder and 0.4g/kg of medlar residual fruit powder are added into daily ration, the growth performance of chicks can be improved and the intestinal digestion function can be enhanced.
Test example 3: determination of chick intestinal mucosa barrier function
The above example 1 group feeding protocol was used to collect the duodenum, jejunum, and ileum of chicks at 28 and 49 days of age, respectively, and determine their relative amounts of barrier-related Occlutin, ZO-1, and MUC2 mRNA.
FIGS. 4 to 9 are graphs showing the effect of matrimony vine and mannooligosaccharide on the relative expression amounts of chicken small intestine Occludin and ZO-1mRNA, notes: * Indicating significant differences (P < 0.05), indicating very significant differences (P < 0.01).
Fig. 10 to 12 effect of matrimony vine and mannooligosaccharide on relative expression amount of chick intestinal MUC2 mRNA, note: * Indicating significant differences (P < 0.05), indicating very significant differences (P < 0.01).
From FIGS. 4 to 12, it is known that the combination of 2g/kg of the fructus Lycii residue fruit powder and 0.4g/kg of the mannooligosaccharide can increase the relative expression levels of jejunum Occludin and ZO-1mRNA of chicks at 28 days old, up-regulate the relative expression levels of intestinal Occludin and MUC2mRNA, up-regulate the relative expression levels of ileum Occludin at 49 days old, ZO-1 and MUC2mRNA and the relative expression level of MUC2mRNA of duodenum; the combination of 1g/kg of the medlar residual fruit powder and 0.4g/kg of the mannooligosaccharide can improve the relative expression quantity of the duodenal ZO-1 and jejunum MUC2mRNA of the chicks at 28 days old, and the combination of 0.5g/kg of the medlar residual fruit powder and 0.4g/kg of the mannooligosaccharide can improve the relative expression quantity of the duodenal ZO-1 and ileum ZO-1mRNA of the chicks at 49 days old.
Test example 4: determination of the pH of the cecal content and the intestinal flora of the chicks
The above example 1 group feeding protocol was used to collect cecum from chicks at 28 and 49 days of age, respectively, and the pH of the intestinal contents was measured with a pH meter, and the numbers of escherichia coli and lactobacillus were counted by plate counting.
The results are shown in Table 6, and it is clear that the combination of three doses of fructus Lycii residual fruit and 0.4g/kg of mannooligosaccharide can reduce the pH of 7-49 day-old cecum content of chicks, and the combination of 2g/kg of fructus Lycii residual fruit powder and 0.4g/kg of mannooligosaccharide has the best effect. TABLE 6 influence of defective Lycium barbarum and mannooligosaccharide on the pH of the cecum contents of chickens
| Grouping | 28 Days old | 49 Days old | |
| CON | 6.77±0.09a | 7.22±0.04a | |
| LBH | 6.45±0.05e | 7.17±0.01AAb | |
| LBM | 6.55±0.12b | 7.15±0.02AAb | |
| LBL | 6.77±0.08b | 7.20±0.01b | |
| LBH+ACT | 6.25±0.06AABBD | 7.02±0.03AABBDEE | |
| LBM+ACT | 6.22±0.06AABBE | 7.06±0.03AABB | |
| LBL+ACT | 6.21±0.04AABBE | 7.07±0.03AABBd | |
| ACT | 6.47±0.37AA | 7.09±0.02AAe |
Note that: the same lower case letters of the shoulder marks are compared with each other, two uppercase letters are extremely obvious in difference (P < 0.01), and one uppercase letter is obvious in difference (P < 0.05).
Fig. 13 to 14 are graphs showing the effects of the defective fruits of matrimony vine and mannooligosaccharides on the numbers of E.coli and Lactobacillus in chickens. And (3) injection: * Indicating significant differences (P < 0.05), indicating very significant differences (P < 0.01).
From FIGS. 13 to 14, it is understood that the combination of 2g/kg and 1g/kg of residual fruit powder of Lycium barbarum with 0.4g/kg of mannooligosaccharide can increase the number of Lactobacillus cecum in 28-day-old chicks; the daily ration is added with the residual fruit powder of the medlar and the combination of the residual fruit powder and 0.4g/kg of mannooligosaccharide, so that the quantity of lactobacillus in the cecum content of the 49-day-old chicks can be increased, and the combination effect of 2g/kg and 0.4g/kg of mannooligosaccharide is the best.
In the whole, when 2g/kg and 1g/kg of medlar residual fruit powder are added into daily ration and are respectively combined with 0.4g/kg of mannooligosaccharide, the intestinal mucosa barrier of the chick can be enhanced.
As can be seen from a combination of the above examples: when 2g/kg of medlar residue fruit powder and 0.4g/kg of mannooligosaccharide are added into daily ration of the egg chicks for combined use, the growth performance of the chicks can be improved by improving average daily gain of the chicks and reducing the feed-weight ratio; the intestinal tract digestive enzyme activity is improved, so that the small intestinal villus height and the villus cryptoratio are improved, and the digestion function of the chicks is enhanced; the intestinal barrier function is enhanced by up-regulating the relative expression levels of intestinal tissue-tight junction protein ZO-1, mucin MUC2, and atresia Occludin mRNA, and the intestinal health is regulated by lowering the cecum pH and increasing the number of Lactobacillus in the cecum contents.
Large group test: based on the above examples, the most suitable combination amount of 2g/kg of medlar residual fruit powder and 0.4g/kg of mannooligosaccharide are selected for feeding. Starting feeding at 7 days old, feeding for 10 days every month, and comparing egg yield and egg quality (eggshell color, eggshell strength, egg shape index, yolk color, etc.) of the laying hens before and after feeding.
Claims (4)
1. A pharmaceutical composition for promoting the growth of egg chicks and regulating intestinal functions, which is characterized in that: the composition consists of medlar and mannooligosaccharide, and the dosage of the medlar and the mannooligosaccharide is 1-2: 0.4.
2. A pharmaceutical composition for promoting the growth of egg chicks and regulating intestinal function according to claim 1, wherein: the composition consists of 2g medlar and 0.4 g mannooligosaccharide.
3. A pharmaceutical composition for promoting the growth of egg chickens and regulating intestinal function according to claim 1 or 2, wherein: the medlar is medlar powder.
4. Use of the pharmaceutical composition of claim 1 for the manufacture of a medicament for promoting growth of an egg chicken and regulating intestinal function.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102987107A (en) * | 2012-09-29 | 2013-03-27 | 凤台县瑞普农业发展有限公司 | Chick feed |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102987107A (en) * | 2012-09-29 | 2013-03-27 | 凤台县瑞普农业发展有限公司 | Chick feed |
Non-Patent Citations (2)
| Title |
|---|
| Effects of mannan-oligosaccharide supplementation on gut health, immunity, and production performance of broilers.;Asif M;Brazilian journal of biology;20220101;第84卷;e250132-e250132 * |
| Goloba kusi (Hornstedtia scottiana [F. Muell.] K. Schum.) fruit as a feed additive to improve the histological structures and growth performance of broiler;Blatama D等人;Veterinary world;20230228;第16卷(第2期);329-340 * |
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