CN105707509B - It is a kind of for preventing and treating the feed addictive of grass carp gill rot - Google Patents

Abstract

The present invention relates to a kind of for preventing and treating the feed addictive of grass carp gill rot, is mainly prepared by the raw material of following parts by weight: 50-100 parts of fish meal, 1-10 parts of lysozyme, 1-10 parts of sea grass polysaccharide.Compared with normal diet additive, aquatic biological feed addictive preparation method of the invention is simple, and compatibility is reasonable, significant in efficacy, can significantly reduce the death rate of fish, experiments have shown that having special efficacy to grass carp gill rot；Since, without western medicine composition, feed addictive has no toxic side effect, and has no drug resistance, pollution-free in additive.

Description

It is a kind of for preventing and treating the feed addictive of grass carp gill rot

Technical field

The present invention relates to field of fodder, and in particular to a kind of feeding additive aquatic animal and its application.

Background technique

It is extremely important to improve human health level to diet structure is improved for fishery -ies product nutritive value with higher.With Health perception is increasingly enhanced, and consumer is higher and higher to the consumption demand amount and quality requirements of fishery -ies product.In recent years, with feed The fast development breeded fish, grass carp are fast because of its speed of growth, it has also become the country main breed fish class, especially hilly pond type reservoir and miniature water Library, master supports, dense stocking grass carp is fairly common.It is continuously improved however as cultivation density, the reasons such as breeding environment pollution are various Fish disease disease incidence also sharply increases, due to grass carp gill rot it is frequent generation and it is difficult to treat and greatly reduce its at Motility rate has seriously affected the economic benefit of professional raising farmer.

Grass carp gill rot is that one kind infects rapid, the longer common fish disease of the course of disease in the fall.There are mainly two types of gill rots, and one Kind is parasitic gill rot, and one is bacterial gill rots.Parasitic gill rot is various gill rots caused by helminth, Helminth breeds in the cheek of grass carp, and stimulation cheek secretes a large amount of mucus, and the enlargement of cheek silk, discoloration seriously affect the breathing of fish；Carefully Bacterium property gill rot is invaded by myxobacter to be caused, and is shown as cheek silk and is rotted, and cheek lid inner surface is congested, and central epidermis is often etched into The duck eye of one circular non-opaque, is commonly called as blank.Sick fish swimming is slow after the onset, and loss of appetite alters trip up and down；Have after death Cheek silk rot, some puts in a skylight at the petal cheek, some.Long-term cultivation practice have shown that, when preventing preferable, be generally not susceptible to Fish disease, if neglected preventions, fish disease easily occurs, and spreads rapidly, and not easy to control.

It mainly prevents and treats the fish diseases such as gill rot in production with antibiotic and disinfectant at present, but is used for a long time and will lead to Extremely serious consequence: (1) abuse of antibiotics causes germ drug resistance to enhance, so that fish disease treatment is more difficult；(2) disinfectant Toxic side effect is big, and local stimulation is strong, is easy carcinogenic, causes fish serious；(3) breeding water body is destroyed, water environment, drop are polluted Low fish body immunity；(4) medicament residue increases, and Safety of Aquatic Products causes anxiety, and quality decline endangers human health.

To solve the above problems, scientific research personnel explores feeding additive aquatic animal new varieties constantly to replace antibiotic and disinfection Agent.Some scholars start to study disease control of aquatic animal from immunology angle, it is believed that immune increase is added in feed Strong agent is enhancing Fish Immunity, a kind of effectively practical method resisted the disease.But the immune system of animal is opposite Stable, the immune function of body can be incorporated into a suitable level by immune system under normal circumstances.Immunopotentiator It is only possible to greatly improve the immune level of the aquatic livestock of immunologic hypofunction, and aquatic products normal for immune function Animal can prevent the true effect that immunopotentiator is influenced due to its immune level is excessively high, condition because of the adjusting of body self immune system And also not the higher the better for immune level, immune level is excessively high to be will lead to and is immunized hyperfunction, and various types of hypersensitivity, inflammation are caused Disease and autoimmune disease.In numerous immunopotentiators, lysozyme and polysaccharide component are because its low medicine is residual, bio-toxicity Small advantage and cause scholars and pay attention to.

Lysozyme (lysozyme) is also known as muramidase (muramidase) or N- acetyl group murein endohydrolase (N- Acetylmuramide glycanohydrlase), it is a kind of water-solubility protein that can be destroyed in pathogen cell wall and stick polysaccharide Enzyme.It is by Fleming nineteen twenty-two found in people's saliva and tears there are the enzymes of dissolution of bacteria cell wall, therefore be named as Lysozyme.Hereafter, in birds, the egg white of poultry and mammal tear, saliva, blood plasma, milk, placenta and body fluid, tissue It is intracellular etc. to have found lysozyme, wherein most abundant (about 0.3%) with content in egg white；In some plants such as cabbage, trailing plants Foretell, there is also lysozymes in fig and microbial body.Research is found in bacillus subtilis (Bacillus subtilis) Dissolution is exactly the predecessor of lysozyme.

Lysozyme itself is a kind of alkaline protein, and under acidic environment, the thermal stability of lysozyme is very strong: pH value 3.0 When 96 DEG C heat treatment 15min be still able to maintain 87% enzymatic activity.Under field conditions (factors), most of lysozyme chemical property are steady Fixed, even if working as pH value acute variation within the scope of 1.2-11.3, the space structure of lysozyme can still be remained unchanged, enzymatic activity Variation is not also significant.But alkaline environment is easy to make the space structure of lysozyme to destroy, poor to the stability of heat at this time, works as place When alkaline pH ranges, temperature can make enzyme activity loss very fast.

Lysozyme can effective hydrolytic bacteria whole cell peptidoglycan, hydrolytic sites are N-acetylglucosamine amine (NAM) L position carbon atom and -acetylmuramic acid (NAG) 4 carbon atoms between β-l, 4 glycosidic bonds, can make bacteria cell wall become pine It relaxes, loses the protective effect to cell, bacterium content overflows and makes cell wall lysis, and last cell dissolution is dead.Lysozyme Why can have very strong lethality to gram-positive bacteria, be exactly because gram positive bacteria cell wall mainly by cytoplasm wall and Phosphoric acid matter composition, main component cytoplasm wall be again heteroglycan and polypeptide composition glycoprotein, and heteroglycan exactly by NAM with NAG with β-Isosorbide-5-Nitrae glucosides key joint, the bacteriolyze enzymatic hydrolysis glycosidic bond make bacteria cell wall rupture and it is dead.And remove from office blue yin Property bacterium inner wall layer be peptide glycan, therefore lysozyme can only destroy Gram-positive cell wall, and act on gram-negative bacteria little.

Lysozyme has a large amount of positive charges under neutral fluid environment, can directly make with the virus protein with negative electrical charge With, with DNA, RNA, desorption base albumen combine to form double salt, make virally inactivated.By the Hela cell training of herpesvirus infection Have in nutrient solution plus after lysozyme and inhibits cell degeneration effect.Lysozyme also can inhibit adenovirus growth, can be used for shingles zoster, the cheek The viral illness treatment such as adenositis, chicken varicella, hepatitis and influenza.

Lysozyme can keep organism physiology to balance, and participate in internal panimmunity reaction, be non-specificity important in body One of immune factor.Lysozyme itself has t cell epitope, can induce the reaction of 2 types (Th2) t helper cell.Oral lysozyme energy Inducing mouse generates systemic Th1 and Th2 immune response, enhances immune function, and therefore, oral lysozyme has to be breathed in control The function of road infection and treatment dysentery.In addition, lysozyme also has the function of activating blood platelet, tissue local blood can be improved and followed Ring obstacle decomposes fester, enhances local defense function, to embody its hemostasis, detumescence and the reparation for accelerating body injury tissue Effect.It is alternatively arranged as a kind of host's resistance factor, shields to tissue local.

Lysozyme can also reduce bacterial endotoxin release in conjunction with the various acidic materials for inducing inflammation, thus in mitigating Endotoxemia occurs, and slows down inflammation generating process, and be known as certain synergistic effect to antibiosis, improves the glutinous polysaccharide generation of periplast It thanks, plays the role of antibacterial anti-inflammatory, repair tissue.Tests prove that with a kind of cellulose copolymer by human lysozyme and albumen water Enzyme coupling is solved, lysozyme antibiotic ability, accelerating wound healing are remarkably improved.

In recent years, going deep into research, people are not only further understood that the mechanism of action of lysozyme, and according to Its bacteriolyze characteristic, has been widely used in medical treatment, food antiseptic, bioengineering and animal productiong.The World Health Organization and Many countries all generally acknowledge the nontoxicitys of lysozymes, and check and approve it and can be used as therapeutic substance and use in some food.As one Nontoxic, the harmless high alkali native protein enzyme of kind, it is safe nutritional feed that lysozyme, which has no effect to zooblast, Additive has non-dedicated property growth hormone effects, can improve alimentary canal microbiologic population, and nutrition digestion is promoted to absorb.

As a kind of important nospecific immunity factor of fish protection pathogen invasion, lysozyme can be hydrolyzed in pathogenic bacteria The alkaline enzyme of glutinous polysaccharide, plays an important role when resisting extraneous bacterial invasion, and it is viscous that it is widely present in the serum of animal, skin In liquid and histoorgan, also there is presence in other biological.Although the research of fish lysozyme is started late, but because its is special Mechanism and its important function in immune defense, cause extensive concern in recent years.Scientific research personnel is in fish bacteriolyze Enzyme gene feature, expression activity, molecular structure and its mechanism of action, the cultivation of Transgenic fish, the exploitation for recombinating lysozyme etc. Aspect has made intensive studies, and is applied to the production in the fields such as food preservative, feed addictive, anti-inflammation and analgesic drugs.

Polysaccharide and its conjugate are to be prevalent in one of plant cell wall and cell ingredient, not due to composed structure Together, physical and chemical feature and bioactivity are also different.Some polysaccharide (such as starch, pectin, cellulose) early have become the mankind Important component in daily life is a kind of important economic raw material, does not have specific bio-active to animal.From 20 The end of the fifties in century makes people start the chemical research of polysaccharide series the discovery of the anticancer effect of fungi polysaccharide.Japan is from 20 Begin one's study the sixties in century the pharmaceutical activity of Basidiomycetes polysaccharide, and develops some famous drugs.Polysaccharide grinds in China Studying carefully starts from the 1970s, in recent years quickly to the research and development of plant polyose, in terms of immune mechanism and clinical application Research develops to molecule, acceptor levels by general observation, and the system of clinical application and health food is developed to by zoopery Make, by mushroom polysaccharide to the more sections of higher plant belong to, multiple groups are knitted, the research of multiple organ.Research finds polysaccharide contained by not kindred plant Class compound is a kind of immunomodulator, it, which has, inhibits tumour growth, immune cell activated, improves body immune function etc. to make With having no toxic side effect to normal cell.Up to the present, the water-soluble polysaccharide from the polysaccharide compound separated in plant It is mostly important.

Polysaccharide has important humidification to body immune system, has multiple target point and is prevalent in specificity and non-spy The characteristics of links of specific immunological activation.A large amount of pharmacology and clinical trial confirm that polysaccharide can not only activate T to drench The panimmunities cells such as bar cell, bone-marrow-derived lymphocyte, macrophage, natural kill (NK) cell, and can be with complement activation system System, effectively facilitates IL-1, IL-2, IL-6, the secretion and expression of the cell factors such as TNF.Immunocompetence, mechanism of action to polysaccharide And the research of structure-activity relationship can not only find active polysaccharide class drug and ancillary drug, but also can carry out to the structure of existing polysaccharide Optimization, to further increase activity.Polysaccharide is since its unique property is gradually recognized by people, and some polysaccharide medicines are Clinically used as the ancillary drug for the treatment of cancer.

Polysaccharose substance has the immunity for improving animal body, prevents virus replication, kills the function such as bacterium, discharge toxin Effect, and have the characteristics that dosage is few, effect is fast, holding effect is waited so long.Research finds that astragalus polyose is added in feed can make carp white The phagocytic rate of cell significantly increases, also more obvious to the facilitation of leukocyte activity.

Enteromorpha (Enteromorpha prolifera) is Chlorophyta, Chlorophyceae, Ulvales, the algae of Enteromorpha. Enteromorpha is a kind of important economical alga, belongs to global waters biology, and natural propagation ability is especially strong, and yield is huge, It is widely distributed in the Shi Zhaozhong in river mouth, middle damp band, can also grow nonparasitically upon another plant on the frond of tangleweed sometimes, China sea area is special It is the green alga resource very abundant of Area of The East China Sea, only ALONG COASTAL FUJIAN annual output is up to 100,000 tons or more.Enteromorpha breaks out frequency Growth and the expansion of geographic range have gradually caused the concern of people.Therefore how this marine resources are preferably utilized, makes it Turning waste into wealth becomes current hot topic.

Polyoses content is 48-55.69%, crude fiber content 8.53-10.2%, protein content 18.91- in Enteromorpha 29.9%, amino acid classes are complete, while also containing minerals and vitamins, are a kind of high protein, high dietary-fiber, low fat Fat, low energy natural ideal nutritional food and health food raw material.Enteromorpha not only has nutritive value abundant, also has Clearing heat and detoxicating, antibacterial anti-inflammatory reduces the effect of cholesterol, enhancing immunity of organisms, swelling diuretic.Enteromorpha deep processed product conduct Green algae breeding feed or feed addictive are used for aquaculture, can effectively facilitate the growth of the aquatic livestocks such as fishes and shrimps, improve and produce Amount promotes quality.

Amino acid is the basic unit of protein, and more than 20 kinds of amino acid being present in protein are aliphatic, fragrance Race and heterocycle three categories.According to contained amino, carboxyl number and whether sulfur-bearing or hydroxyl, during aliphatic amino acid can be divided into again Property, acidity, alkalinity, hydroxyl and sulfur-containing amino acid are raised.It can also classify from nutritional point, amino contained in feed protein Acid is divided into essential amino acid and nonessential amino acid.So-called essential amino acid i.e. cannot synthesize in animal body or aggregate velocity and Quantity is not able to satisfy normal growth needs, it is necessary to supply from feed.Nonessential amino acid synthesizes more in animal body Or need it is less, without supplying the needs for being also able to maintain animal normal growth from feed.

Methionine mainly has the hydroxy analogs (MHB) and its calcium salt of DL- METHIONINE and methionine.The nutrition balance of MHB It is equivalent to 80% or so of DL- METHIONINE.Methionine content in vegetable protein is seldom, and birds will make full-fledged needs Absorb enough methionine (sulfur-containing amino acid).The dosage that Japan adds methionine in the insufficient chicken feed of fish meal is 0.1% ~0.2%.American-European countries adds egg ammonia because not using fish meal generally in the diet that corn adds soya-bean cake or small Meccah soya-bean cake The dosage of acid is 3 times of Japanese dosage.The U.S. is often using MHB as methionine additive.Adding methionine in diet can be albumen Matter is consolidated to the level of fish meal, and 1 kilogram of methionine of every addition can replace 50 kilograms of fish meal.Such as based on corn and Oilseed meal Daily ration in add methionine reference ratio be broiler chicken and laying hen 0.1%~0.2%, growing and fattening pigs be 0.02%~0.05%, Piglet is 0.05%~0.10%.

Content of the lysine in vegetable protein be not high, and containing on a small quantity in soya-bean cake, corn content is seldom.Therefore lacking fish Addition lysine is needed in powder and the insufficient diet of soya-bean cake.Lysine is the 1st limiting amino acid of animal.According to test, in animal Lysine is added in diet, and the protein level 2%~3% in diet can be improved.1 kilogram of bad ammonia of every addition in broiler fodder Acid can save 25 kilogram of feed, can improve lean meat percentage using lysine in the diet of pig.It is added in pig fowl diet and relies ammonia The ratio of acid, depending on the level of lysine in diet, generally 0.1%~0.2%, lack fish meal and the insufficient feeding of soya-bean cake Material can be added to 0.2%, and general piglet and growing and fattening pigs are 0.1%.

As vitamine supplement is widely applied in feed industry rapid development in feed.Vitamine supplement has natural With synthesis, the vitamin as feed addictive is mainly using synthesis vitamin.There are many vitamine supplement type, commonly Vitamine supplement has 14 kinds, can be divided into liposoluble vitamin and soluble vitamin preparation two major classes by its dissolubility.It is common Liposoluble vitamin include vitamin A, vitamine D3, vitamin E and four kinds of vitamin K, water soluble vitamin includes dimension life Plain B race, vitamin C, niacin, pantothenic acid etc..Although vitamin demand is more micro, each vitamin all plays other substances Irreplaceable special dietary physiological action.If animal lack or cross Multivitamin growth and development will be generated significantly it is bad after Fruit.Therefore, it is necessary to add the desired amount of vitamin, in daily feed for animal body needs.

Vitamin A also known as retinene, antixerophthalmic vitamin are containing β-root of Dahurain angelica ketone ring unsaturated monohydric alcohol, by different At diene building-block molecule biosynthesis.Pure vitamin A is light yellow crystal, to thermostabilization when anoxic, to thermally labile when aerobic, And easily by ultraviolet damage.Vitamin A exists only in animal body, there was only provitamin A in plant --- and carrotene and class are recklessly Radish element.Vitamin A includes A1 and two kinds of A2, is existed in the liver of lactation and saltwater fish, and A2 is present in the liver of fresh-water fishes In, the two can be digested and assimilated directly by animal body.α, β, gamma carotene and luteole are in animal intestine parietal cell and liver Vitamin A can be converted into through carrotene enzyme effect in dirty, mammary gland.Vitamin A can irritate the growth of experimental animal, can also irritate The synthesis of DNA in many tissues；Vitamin A is adjustable the immune function of animal body；It can restore the growth of certain cell types Inhibition contact, and improve sticking between cell；The derivative of vitamin A is alternatively arranged as sugar in special glycoprotein synthesis Carrier.

Vitamin C is also known as ascorbic acid, exists in two forms in nature, i.e. reduction form L-AA and oxygen Change form L- deoxidation ascorbic acid, most of vitamin C exist in the form of restoring.During Fodder making, ascorbic reality Border additive amount should be greater than theory demands amount.Currently, mainly foundation has two o'clock to actual interpolation amount of the vitamin C in feed: first is that The nutritional requirement of relevant animal；Second is that the stability of the kind used.Recommendation dosage of the vitamin C in feed generally needs 4-5 times of the amount of asking.Tonic material, livestock and poultry resisting stress daily ration and fast elongated meat are opened in increasingly developed aquatic feeds, mammal The higher vitamin C of Add-On Stabilization or vitamin C derivatives in the mineralization special daily ration of fowl bone, it has also become feeding vitamin An important factor for C demand growth.

Vitamin E also known as tocopherol, C antisterility vitamin are the similar phenols of one group of biologically active chemical structure Compound is primarily present in vegetable oil.Natural vitamin E has 8 kinds, it may be assumed that α, β, γ, Delta-Tocopherol and α, β, γ, δ-are raw Trienol is educated, is the derivative that benzene is total to dihydropyran.Vitamin E is easily oxidized and protects other substances not oxidized, is Most effective antioxidant in animal body.It can fight the peroxidization of unsaturated fatty acid in biological film phospholipid, avoid rouge The generation of peroxide in matter, protects the structure and function of biomembrane；Vitamin E can also be cooperateed with selenium passes through glutathione peroxide Change enzyme play antioxidation, prevent necrosis of liver tissue and muscle damage, maintain red blood cell stability and capillary it is complete Whole property；Alpha-tocopherol can influence the formation of biomembrane by influencing the structure of membrane phospholipid；The reproductive capability of vitamin E and animal It is closely related, have and promote gonad development, facilitates the effects of becoming pregnant and preventing miscarriage；Vitamin E can promote the conjunction of ferroheme At；The vitamin E of high dose can also promote the generation of immunoglobulin, improve animal body to the resistance of disease, enhancing is anti- Stress.

Vitamin K is the general name of a kind of antihaemorrhagics compound containing menadione structure.Vitamin K can be divided into two major classes Compound: K1, K2 and K3 can be extracted in animal and plant body；Another kind of is water soluble compound K4, is artificial chemical synthesis.Dimension Raw element K is that vitamin necessary to factor is formed in animal body, can promote dimension life necessary to liver synthesis factor Element can promote liver to synthesize factor (factor II)；Adjust the synthesis of in addition several factor VIIs, Ⅸ and Ⅹ；It removes Outside hemoglutination, vitamin K is also possible to dependent protein matter and peptide participates in calcium metabolism.

It is used to prevent and treat the application of grass carp gill rot currently, combining with sea grass polysaccharide lysozyme there is not been reported.

Summary of the invention

The anti-gill rot ability of grass carp, and nontoxic pair can be effectively improved technical problem to be solved by the invention is to provide a kind of Effect, pollution-free, the fish feed additive having no drug resistance and the feed containing the additive.

In addition, the present invention also provides a kind of application methods of above-mentioned additive.

The present invention solves the above problems the technical scheme adopted is that providing a kind of feed addition for preventing and treating grass carp gill rot Agent is mainly made of the component of following parts by weight: 50-100 parts of fish meal, 1-10 parts of lysozyme, 1-10 parts of sea grass polysaccharide.

Preferably, in the feed addictive, the parts by weight of each component are as follows: 50 parts of fish meal, 1-4 parts of lysozyme, Enteromorpha is more It is 1-4 parts sugared.

It is furthermore preferred that in the feed addictive, the parts by weight of each component are as follows: 50 parts of fish meal, 1 part of lysozyme, Enteromorpha is more 3 parts of sugar.

Preferably, the raw material further comprises other active components.

It is furthermore preferred that the other active components are selected from one of amino acid, vitamin etc. or a variety of.

Most preferably, the amino acid is methionine and/or lysine etc., the vitamin be vitamin A, vitamin C, Vitamin E and/or vitamin K etc..

The present invention also provides a kind of application of above-mentioned feed addictive in the feed of preparation prevention and treatment grass carp gill rot.

Preferably, in the feed of 100 parts by weight, the feed addictive containing 1-5 weight %.

The invention has the advantages that:

It is surprising that by repeated tests, present inventors have unexpectedly found that by lysozyme and polysaccharose substance Enteromorpha Polysaccharide, which is applied in combination, has the effect of synergy for the prevention and treatment of grass carp gill rot, and cooperation other active components using effect is more It is good.

Compared with normal diet additive, aquatic biological feed addictive preparation method of the invention is simple, and compatibility is reasonable, It is significant in efficacy, the death rate of fish can be significantly reduced, experiments have shown that having special efficacy to grass carp gill rot；Due in additive without west Medicine ingredient, therefore feed addictive has no toxic side effect, and has no drug resistance, it is pollution-free.

Specific embodiment

Below with reference to embodiment, the present invention will be further explained, and embodiments of the present invention are not limited thereto.Under It states experimental method used in embodiment unless otherwise specified, is conventional method.

The extraction of embodiment 1, sea grass polysaccharide

It takes Enteromorpha dry product to add the flooding of 50 times of weight, the pH value of aqueous solution is adjusted before extracting to 5 or so, is extracted at 60 DEG C 2 hours, supernatant is taken to be concentrated in vacuo, with 95% ethanol precipitation of 5 times of volume of the concentrated liquid, sediment is re-dissolved in suitable quantity of water, is used Sevag method takes off albumen, and appropriate 95% ethanol precipitation is dried in vacuo to obtain sea grass polysaccharide.

The preparation of embodiment 2, feed addictive

50 parts by weight of fish meal are taken, feed addictive A1 is made in 5 parts by weight of lysozyme after mixing.

50 parts by weight of fish meal are taken, feed addictive is made after mixing in 5 parts by weight of sea grass polysaccharide that embodiment 1 is prepared A2。

Take 50 parts by weight of fish meal, 4 parts by weight of lysozyme, 1 parts by weight of sea grass polysaccharide that embodiment 1 is prepared, after mixing Lysozyme and sea grass polysaccharide weight ratio is made as the feed addictive A3 of 4:1.

50 parts by weight of fish meal, 2.5 parts by weight of lysozyme are taken, 2.5 parts by weight of sea grass polysaccharide that embodiment 1 is prepared are mixed Lysozyme and sea grass polysaccharide weight ratio are made after conjunction as the feed addictive A4 of 1:1.

Take 50 parts by weight of fish meal, 1.25 parts by weight of lysozyme, 3.75 parts by weight of sea grass polysaccharide that embodiment 1 is prepared, Lysozyme and sea grass polysaccharide weight ratio are made after mixing as the feed addictive A5 of 1:3.

Take 50 parts by weight of fish meal, 1 parts by weight of lysozyme, 4 parts by weight of sea grass polysaccharide that embodiment 1 is prepared, after mixing Lysozyme and sea grass polysaccharide weight ratio is made as the feed addictive A6 of 1:4.

Using gel method, the freeze-dried particle that diameter 0.5mm is made, i.e., lysozyme used in above-described embodiment is Product are coated with for lysozyme.

The application of embodiment 3, the feed addictive comprising lysozyme and sea grass polysaccharide in anti-grass carp gill rot

To verify application effect of the invention, feed addictive of the invention is tested in the grass carp in 7 fishing grounds.It will Grass carp is divided into experimental group (A1-A6) and control group, and every group is 200 tails.Wherein, the daily ration of experimental group is according to feed total weight The 1% above-mentioned feed addictive of addition, the daily ration of control group is the fish meal for not adding the identical weight of above-mentioned feed addictive, respectively Group fish feeds 5 times daily, and feeding carries out infection experiment after 60 days, and experimental group and control group, which are injected, can lead to causing a disease for gill rot Bacterium is observed continuously 7 days, the The dead quantity of grass carp in statistical experiment group and control group, and calculates the death rate (being shown in Table 1).

Table 1

	Infect death toll	The death rate
			Control group	134	67%
Lysozyme group	98	49%
			Sea grass polysaccharide group	86	43%
Lysozyme+sea grass polysaccharide group (4:1)	36	18%
			Lysozyme+sea grass polysaccharide group (1:1)	31	15.5%
Lysozyme+sea grass polysaccharide group (1:3)	12	6%
			Lysozyme+sea grass polysaccharide group (1:4)	26	13%

Feed addictive of the invention can effectively improve the immune function of grass carp it can be seen from the comparative experiments of embodiment 3 Can, enhance resistance to infection, reduces the death rate of grass carp, especially lysozyme and being applied in combination for sea grass polysaccharide produces Synergy reduces the usage amount of drug.In the case where active constituent total amount remains unchanged, feed addictive of the invention Significant effect compared with the anti-grass carp gill rot of feed addictive that lysozyme or sea grass polysaccharide is used alone improves, and the fish death rate is extremely 25% is reduced less, and the feed addictive effect that wherein lysozyme and sea grass polysaccharide weight ratio are 1:3 is the most prominent, and fish are dead The rate of dying at least reduces 37%, produces and is difficult to expected excellent effect.

Claims (6)

1. a kind of for preventing and treating the feed addictive of grass carp gill rot, which is characterized in that mainly by the raw material system of following parts by weight It is standby to form: 50 parts of fish meal, 1 part of lysozyme, 3 parts of sea grass polysaccharide.

2. feed addictive according to claim 1, which is characterized in that the raw material further comprise other activity at Point.

3. feed addictive according to claim 2, which is characterized in that the other active components are selected from amino acid, dimension One of raw element is a variety of.

4. feed addictive according to claim 3, which is characterized in that the amino acid be methionine and/or lysine, The vitamin is vitamin A, vitamin C, vitamin E and/or vitamin K.

5. application of the described in any item feed addictives of claim 1-4 in the feed of preparation prevention and treatment grass carp gill rot.

6. application according to claim 5, which is characterized in that in the feed of 100 parts by weight, contain 1-5 weight % Feed addictive.