CN105454695A - Feed additive for preventing and curing porcine diarrhea - Google Patents

Abstract

The invention relates to a feed additive for preventing and curing porcine diarrhea. The feed additive is mainly prepared from, by weight, 50-100 parts of bone meal, 1-10 parts of lysozyme and 1-10 parts of coriolus versicohr polysaccharide. Compared with an ordinary feed additive, according to the feed additive for preventing and curing the porcine diarrhea, a preparation method of the livestock biological feed additive is simple, compatibility is reasonable, the curative effect is significant, the diarrhea rate of livestock can be obviously decreased, and it is proved through experiments that the feed additive has the special effect on preventing and curing the porcine diarrhea; due to the fact that the additive does not contain western medicine components, the feed additive does not have toxic and side effect, the drug resistance does not exist, and pollution does not exist.

Description

A kind of feed addictive for preventing and treating diarrhea of pigs

Technical field

The present invention relates to field of fodder, be specifically related to a kind of domestic animal feed additive and application thereof.

Background technology

Livestock feed additive refers to a small amount of or micro substance added in livestock feed production and processing, use procedure, in livestock feed consumption seldom but effect significantly.Livestock feed additive is the raw material that the industry of modern livestock feed must use, and to the basic livestock feed nutritive value of strengthening, improve breeding performonce fo animals, ensure animal health, save livestock feed cost, improving the aspects such as domestic animals product quality has obvious effect.

Diarrhea of pigs is sick to be produced because pig infects virus, and the pig at various age can infection morbidity.The incidence of disease of suckling pig, feeder pig or fattening pig is very high, and be especially injured with suckling pig the most serious, the variation of the sow incidence of disease is very large, is about 15-90%.Sick pig is major source of infection.Virus is present in intestinal villus epithelial cell and lymphonodi mesenterici, after discharging with ight soil, and contaminated environment, feed, drinking-water, the vehicles and apparatus etc. and infect.If a pig farm has many nest piglets to be born or wean successively, virus can constantly infect the weanling pig losing maternal antibody, makes this disease in endemicity, and in this breeding farm, pig epidemic diarrhea can cause the weaning period intractable diarrhea of 5-8 piglet in age in week.Diarrhea of pigs is generally 5-8 days sick incubation period, and artificial challenge is 8-24 hour incubation period.Main clinical symptoms are watery diarrhea, or have vomiting between diarrhoea.Vomit multiple be born in food or suck the breast after.The weight of symptom is variant with the size at age, and the age is less, and symptom is heavier.In one week age there is 3-4 days after diarrhoea in newborn piglet, and present serious dehydration and dead, the death rate can reach 50%, and the highest death rate reaches 100%.Sick temperature of pig body is normal or slightly high, and spirit is depressed, anorexia or absolutely useless.Wean pig, sow are often about a week of down in spirits, apocleisis and persistent diarrhea, and recover normal gradually.Retarded growth after minority pig recovers.Fattening pig is all suffered from diarrhoea after raising infection with circle, rehabilitation after a week, death rate 1%--3%.Adult Pig symptom is comparatively light, and the only performance vomiting had, severe one watery diarrhea 3-4 days can self-healing.

Antibiotic is the main feed addictive for the treatment of diarrhea of pigs disease, and it is while preventing and curing diseases, and also has the effect promoting growth of animal, improve food conversion ratio.In addition, be also improved the quality of animal product, the excrement reducing animal is smelly, thus improves the effects such as feeding environment.The dosage that different types of antibiotic is used for feed interpolation and the growth promoting effects effect had are not quite similar.But on the whole, consumption generally adds between 10 ~ 50g antibiotic in feed per ton.In general effect can improve pig chicken throughput rate and efficiency of feed utilization 10% ~ 15%, reduce the death rate 5%, wherein will slightly lower than the effect to pig to the effect of chicken.For salinomycin, it can improve 37 ~ 76 percentage points to the incubation rate of broiler chicken, and average weight gain can improve 5% ~ 38%, and food consumption reduces by 2% ~ 37%.Add result of the test display at the layer chicken feed carried out with salinomycin, its average age at first calving increases by 13 days, and weight of laying eggs improves 8.1%, and spawning rate improves 3.5%.As can be seen here, antibiotic is obviously as the effect that feed addictive is applied.

Along with the increase day by day that antibiotic uses as feed addictive, also occurred the suggestion opposing antibiotic to use as feed addictive, and this opposing views are further strong in recent years.The personnel being mainly engaged in medical antibiotic investigation and application dissented.They think and use antibiotic to the long-time low dosage of edible animal, no matter be used as feed addictive or prophylactic, all ecological environment more can be caused the screening of drug-fast bacteria than short time heavy dose medicine for treatment, and drug resistant gene can transmit mutually between bacterium in bacterium and the ecosystem in bacterium, fauna in crowd in bacterium, can cause thus pathogenic bacteria produce drug resistance, thus cause human and animal catch treatment failure.In fact, since antibiotic is found, really become its service life shorter and shorter.When penicillin just enters clinical practice, its using dosage is only several, ten units, to 60 ~ seventies, the general intramuscular injection for treating dosage of penicillin on medical and clinical is 100,000 units, more universal along with penicillin application, and its using dosage has to increase rapidly.Up till now, use the intramuscular injection dosage of 800,000 units to treat clinically, effect is even not as good as the past.In the antibiotics constantly occurred, the Clinical practice life-span all becomes shorter and shorter.The appearance of this phenomenon, makes increasing to the demand of antibiotics clinically.Very huge owing to finding the input needed for a kind of antibiotics, this phenomenon causes extreme loss economically.Moreover, along with the increase of antibiotics, find that the difficulty of antibiotics has become increasing.

For solving the problem, scientific research personnel constantly explores domestic animal feed additive new varieties to replace antibiotic.Some scholars start to study from immunology angle domestic animal disease control, think that in feed, add immunopotentiator is strengthen domestic animal immunologic function, the method for a kind of effective practicality of opposing diarrhoeal diseases.But the immune system of animal is metastable, the immunologic function of body can be incorporated into a suitable level by immune system under normal circumstances.Immunopotentiator only may improve the immune level of the domestic animal of immunologic hypofunction greatly, and the true effect of immunopotentiator can be affected because the adjustment of body self immune system stops its immune level too high for the normal domestic animal of immunologic function, moreover immune level is also not more high better, the too high meeting of immune level causes immunity hyperfunction, causes various types of hypersensitivity, inflammation and autoimmune disease.In numerous immunopotentiators, lysozyme and polysaccharide composition cause scholars because of its advantage that low medicine is residual, bio-toxicity is little and pay attention to.

Lysozyme (lysozyme), also known as muramidase (muramidase) or N-acetyl group murein endohydrolase (N-acetylmuramideglycanohydrlase), is a kind of water-solubility protein enzyme that can destroy glutinous polysaccharide in pathogen cell wall.It is the enzyme that there is dissolution of bacteria cell membrane found in people's saliva and tears in nineteen twenty-two by Fleming, therefore called after lysozyme.After this, lysozyme has been found, wherein with content in egg white the abundantest (about 0.3%) in the egg white of birds, poultry and mammal tear, saliva, blood plasma, milk, placenta and body fluid, histocyte etc.; At some plants as also there is lysozyme in cabbage, radish, fig and microbial body.Dissolving in research discovery bacillus subtilis (Bacillussubtilis) is exactly the predecessor of lysozyme.

Lysozyme itself is a kind of alkaline protein, and under sour environment, the heat endurance of lysozyme is very strong: during pH value 3.0,96 DEG C are heated the enzymatic activity that 15min still can keep 87%.Under field conditions (factors), most of lysozyme stable chemical nature, even if when pH value acute variation within the scope of 1.2-11.3, the space structure of lysozyme still can remain unchanged, and the change of enzymatic activity is not remarkable yet.But alkaline environment is easy to make the space structure of lysozyme to destroy, now to the less stable of heat, when being in alkaline pH ranges, temperature can make enzyme activity lose quickly.

Lysozyme can effective hydrolytic bacteria whole cell peptidoglycan; its hydrolytic sites is the β-l between the l position carbon atom of 2-Acetamido-2-deoxy-D-glucose amine (NAM) and 4 carbon atoms of-acetylmuramic acid (NAG); 4 glycosidic bonds; bacteria cell wall can be made to become lax; lose the protective effect to cell; bacterium content overflows and makes cell wall lysis, and last cytolysis is dead.Why lysozyme can have very strong lethality to gram-positive bacteria, because the cell membrane of gram positive bacteria is mainly made up of kytoplasm wall and phosphoric acid matter, its main component kytoplasm wall is again the glycoprotein that heteroglycan and polypeptide form, and heteroglycan just by NAM and NAG with β-1,4 glycosidic bonds connections, this glycosidic bond of lysozyme catalyzing hydrolysis makes bacteria cell wall break and dead.And gram-negative bacteria inner wall layer is peptide glycan, therefore lysozyme can only destroy Gram-positive cell wall, and little to gram-negative bacteria effect.

Lysozyme under neutral fluid environment with a large amount of positive charge, can with the virus protein direct effect with negative electrical charge, combine with DNA, RNA, desorption base albumen formation double salt, makes virally inactivated.T suppression cell denaturation is had after adding lysozyme in by the Hela cell culture fluid of herpesvirus infection.Lysozyme also can suppress adenovirus to grow, and can be used for the viral illness treatment such as herpes zoster, mumps, chicken varicella, hepatitis and influenza.

Lysozyme can keep organism physiology to balance, and participates in panimmunity reaction in body, is one of nospecific immunity factor important in body.Lysozyme itself has t cell epitope, and 2 types (Th2) t helper cell can be induced to react.Oral lysozyme energy inducing mouse produces general Th1 and Th2 immune response, strengthens immunologic function, and therefore, oral lysozyme has the function controlling the infection of the upper respiratory tract and treatment dysentery.In addition, lysozyme also has the hematoblastic function of activation, can improve tissue local blood circulation disorder, decomposes fester, strengthens local defense function, thus embodies the repair that it stops blooding, subsides a swelling and accelerate body injury tissue.It also can be used as a kind of host's resistance factor, shields to tissue local.

Lysozyme also can be combined with the various acidic materials bringing out inflammation, reduces bacterial endotoxin release, thus alleviates endotoxemia generation, slow down inflammation generating process, and certain synergistic effect is have to antibiosis, improve the glutinous polysaccharide metabolism of periplast, play the effect of anti-inflammation, repair tissue.Prove through test, with a kind of cellulose copolymer by human lysozyme and proteolytic enzyme coupling, lysozyme antibiotic ability can be significantly improved, accelerating wound healing.

In recent years, along with going deep into of research, people not only have the mechanism of action of lysozyme and further understand, and according to its bacteriolyze characteristic, have been widely used in medical treatment, food antiseptic, bioengineering and animal productiong.The World Health Organization and many countries all generally acknowledge the nontoxicity of lysozyme, and check and approve it and can be used as therapeutic substance and use in some food.As a kind of nontoxic, harmless high alkali native protein enzyme, lysozyme has no effect to zooblast, is safe nutritional feed additive, has non-dedicated growth hormone effects, can improve alimentary canal microbiologic population, promotes that nutrition digestion absorbs.

As the important nospecific immunity factor of one of domestic animals protection pathogen invasion, lysozyme can be hydrolyzed the alkaline enzyme of glutinous polysaccharide in pathogenic bacteria, play an important role when resisting extraneous bacterial invasion, it is extensively present in the serum of animal, Skin mucus and histoorgan, in other biological, also have existence.Although the research of domestic animals lysozyme is started late, the mechanism special because of it and the important function in immune defense thereof, cause in recent years and pay close attention to widely.Scientific research personnel conducts in-depth research in exploitation of domestic animals lysozyme gene feature, expression activity, molecular structure and the mechanism of action thereof, restructuring lysozyme etc., and is applied to the production in the fields such as food preservative, feed addictive, anti-inflammation and analgesic drugs.

Polysaccharide and conjugate thereof are a kind of compositions be prevalent in plant cell wall and cell, because composition structure is different, its physics and chemistry feature and biologically active also different.Some polysaccharide (as starch, pectin, cellulose etc.) becomes the important component part in mankind's daily life already, is a kind of important economic raw material, does not have specific bio-active to animal.People are made to start the chemical research of polysaccharide series from the discovery of late 1950s to the anticancer effect of fungi polysaccharide.Japan begins one's study from the sixties in 20th century pharmaceutically active of Basidiomycetes polysaccharide, and develops some famous medicines.China starts from 20 century 70s to the research of polysaccharide, very fast to the research and development of plant polyose in recent years, research in immune mechanism and clinical practice develops into molecule, acceptor levels by general observation, the making of clinical practice and health food is developed into, by the research of mushroom polysaccharide to higher plant many sections genus, many tissues, multiple organ by zoopery.Research finds that polysaccharide compound contained by kindred plant is not a kind of immunomodulator, and it has Tumor suppression growth, immune cell activated, improves the effects such as body immune function, to not normal cells side effect.Up to the present, in the polysaccharide compound separated from plant, water-soluble polysaccharide is the most important.

Polysaccharide has important humidification to body immune system, has Mutiple Targets and is prevalent in the feature of links of specificity and nospecific immunity activation.A large amount of pharmacology and clinical trial confirm, polysaccharide not only can the panimmunity cell such as activated T lymphocytes, bone-marrow-derived lymphocyte, macrophage, NKT (NK) cell, and can complement activation system, effective promotion IL-1, IL-2, the secretion of the cell factors such as IL-6, TNF and expression.Both can find active polysaccharide class medicine and ancillary drug to the research of the immunocompetence of polysaccharide, mechanism of action and structure-activity relationship, and can be optimized the structure of existing polysaccharide again, thus improve activity further.Polysaccharide due to its uniqueness character gradually be familiar with by people, some polysaccharide medicines use as the ancillary drug of Therapeutic cancer clinically.

Polysaccharose substance have improve animal body immunity, stop the effect such as virus replication, kill bacteria, discharge toxin, and have that consumption is few, effect is fast, holding effect waits so long feature.Research finds that in feed, add astragalus polyose can make the leukocytic phagocytic rate of carp significantly raise, also comparatively obvious to the facilitation of leukocyte activity.

Rainbow conk (Coriolusverscidor) belongs to Basidiomycetes rainbow conk and belongs to, and is the Chinese herbal medicine resource of China's preciousness.All coriolan (Coriolusversciclorpolysaccharides can be produced with external in prepared from coriolus versicolor mycelium, CVP), it is a kind of proteinaceous Polysaccharide-Peptide complex, main component is based on the glucan peptide complexes of β-(1-4), β-(1-3) or β-(1-4), β-(1-6) glycosidic bond, on have β-(1-3) or β-(1-6) to combine branch, polyoses content 30%-60%, Binding Protein 1 5%.

The research of Chinese scholars to mouse, shrimps and fish shows, coriolan can transfer the immune system of animal comprehensively, and having and improve the function such as immunocompetence and anti-inflammatory, antiviral, radioresistance, is a kind of good BRM and immunopotentiator.Research finds that the coriolan of interpolation 0.1% in feed can strengthen the phagocytosis of Haemocyte of Macrobrachium Nipponense significantly.

Amino acid is the base unit of protein, and 20 several amino acids be present in protein are aliphatic, aromatic series and heterocycle three major types.Whether according to the number of contained amino, carboxyl and sulfur-bearing or hydroxyl, aliphatic amino acid can be divided into again neutrality, acidity, alkalescence, hydroxyl and sulfur-containing amino acid to raise.Also can classify from nutritional point, be essential amino acid and nonessential amino acid Amino acid score contained in feed protein.Namely so-called essential amino acid can not synthesize in animal body or aggregate velocity and quantity can not meet normal growth needs, must supply from feed.Namely the acid of nonessential nitrogen base synthesized more in animal body or needed less, need not supply the needs that also can keep animal normal growth from feed.

Methionine mainly contains hydroxy analogs (MHB) and the calcium salt thereof of DL-METHIONINE and methionine.The nutrition balance of MHB is equivalent to about 80% of DL-METHIONINE.Methionine content in vegetable protein is little, and bird will make full-fledged needs absorb enough methionine (sulfur-containing amino acid).The consumption that Japan adds methionine in the chicken feed of fish meal deficiency is 0.1% ~ 0.2%.American-European countries is not because generally using fish meal, and the consumption thus adding methionine in the diet of corn with soya-bean cake or little Meccah soya-bean cake is 3 times of Japanese consumption.The U.S. is often methionine additive with MHB.In diet, add methionine the level of protein fortification to fish meal, often to add 1 kilogram of methionine, 50 kilograms of fish meal can be replaced.As based on the reference ratio of adding methionine in the daily ration of corn and Oilseed meal being, broiler and layer 0.1% ~ 0.2%, growing and fattening pigs are 0.02% ~ 0.05%, and piglet is 0.05% ~ 0.10%.

The content of lysine in vegetable protein is not high, and containing a small amount of in soya-bean cake, corn content is little.Therefore need to add lysine in the diet lacking fish meal and soya-bean cake deficiency.Lysine is animal the 1st limiting amino acid.According to test, in animal diet, add lysine, the protein level 2% ~ 3% in diet can be improved.Often add 1 kilogram of lysine in broiler fodder, 25 kilogram of feed can be saved, in the diet of pig, use lysine to improve lean meat percentage.In pig fowl diet, add the ratio of lysine, depending on the level of lysine in diet, be generally 0.1% ~ 0.2%, lack the feed of fish meal and soya-bean cake deficiency, can add 0.2% to, general piglet and growing and fattening pigs are 0.1%.

Along with feed industry develops extensive use vitamine supplement in feed rapidly.Vitamine supplement has natural in synthesis, and the vitamin as feed addictive mainly adopts synthetic vitamin.Vitamine supplement kind is a lot, and conventional vitamine supplement has 14 kinds, can be divided into liposoluble vitamin and the large class of soluble vitamin preparation two by its dissolubility.Conventional liposoluble vitamin comprises vitamin A, vitamine D3, vitamin E and vitamin K four kinds, and water soluble vitamin comprises vitamin B complex, vitamin C, nicotinic acid, pantothenic acid etc.Although vitamin demand is more micro-, each vitamin all plays the irreplaceable special dietary physiological action of other materials.If animal lacks or crosses Multivitamin will produce obvious adverse consequences to growing.Therefore, the vitamin of aequum must be added in daily feed, need for animal body.

Vitamin A has another name called retinene, antixerophthalmic vitamin, is the unsaturated monohydric alcohol containing β-root of Dahurain angelica ketone ring, by the biosynthesis of isoprene building-block molecule.Pure vitamin A is light yellow crystal, to thermally-stabilised during anoxic, to thermally labile during aerobic, and easily by ultraviolet damage.Vitamin A exists only in animal body, only has provitamin A in plant---carrotene and carotenoid.Vitamin A comprises A1 and A2 two kinds, and be also present in the liver of lactation and saltwater fish, A2 is present in the liver of fresh-water fishes, and the two can directly be digested and assimilated by animal body.α, β, gamma carotene and luteole are in animal intestine parietal cell and liver, can be converted into vitamin A through carotinase effect in mammary gland.Vitamin A can irritate the growth of animal used as test, also can irritate the synthesis of DNA in many tissues; Vitamin A can regulate the immunologic function of animal body; The growth inhibition contact of some cell type can be recovered, and improve sticking between cell; The derivative of vitamin A also can be used as the carrier of sugar in the synthesis of special glycoprotein.

Vitamin C, also known as ascorbic acid, exists in two forms at occurring in nature, i.e. reduction form L-AA and oxidised form L-deoxidation ascorbic acid, and most of vitamin C exists with form of reducing.In Fodder making process, ascorbic actual addition should be greater than theory demands amount.At present, the actual addition Main Basis of vitamin C in feed has 2 points: one is the nutritional requirement of relevant animal; Two is the stability of kind used.The recommendation consumption of vitamin C in feed is generally the 4-5 of demand doubly.At growing aquatic feeds, mammiferously open the vitamin C or vitamin C derivatives that in tonic material, livestock and poultry resisting stress daily ration and the mineralization special daily ration of fast elongated poultry bone, Add-On Stabilization is higher, become the key factor that feeding vitamin C requirements increases.

Vitamin E has another name called tocopherol, C antisterility vitamin, is one group and has the similar phenolic compound of bioactive chemical constitution, be mainly present in vegetable oil.Natural vitamin E has 8 kinds, that is: α, β, γ, Delta-Tocopherol and α, β, γ, δ-tocotrienols, is all the derivative that benzene is total to dihydropyran.Vitamin E is very easily oxidized and protect other materials not oxidized, is the most effective antioxidant in animal body.In its energy antibiont membrane phospholipid, the peroxidization of unrighted acid, avoids the generation of peroxide in lipid, protects biomembranous 26S Proteasome Structure and Function; Vitamin E also can play antioxidation with selenium is collaborative by glutathione peroxidase, prevents necrosis of liver tissue and muscle damage, maintains the integrality of erythrocytic stability and capillary; Alpha-tocopherol affects biomembranous formation by affecting the structure of membrane phospholipid; The reproductive capability of vitamin E and animal is closely related, has promotion gonad development, facilitates effects such as becoming pregnant and prevent miscarriage; Vitamin E can promote the synthesis of ferroheme; The vitamin E of high dose can also the generation of Promote immunity globulin, improves animal body to the resistance of disease, strengthens anti-stress effect.

Vitamin K is the general name that a class contains the antihaemorrhagics compound of menadione structure.Vitamin K can be divided into two large compounds: K1, K2 and K3, can extract in animal and plant body; Another kind of is water soluble compound K4, is artificial chemical synthesis.Vitamin K forms the necessary vitamin of factor in animal body, can promote that liver synthesizes the necessary vitamin of factor, can promote that liver synthesizes factor (factor II); Regulate other several factor VII, Ⅸ and Ⅹ synthesis; Except hemoglutination, vitamin K also may dependent protein matter and peptide participation calcium metabolism.

At present, there is not been reported lysozyme and coriolan to be combined the application being used for preventing and treating diarrhea of pigs.

Summary of the invention

Technical problem to be solved by this invention is to provide one effectively can improve diarrhea of pigs, and has no side effect, pollution-free, the pig feed additive had no drug resistance and the feed containing this additive.

In addition, the present invention also provides a kind of application process of above-mentioned additive.

The present invention's adopted technical scheme that solves the problem is that provide a kind of feed addictive preventing and treating diarrhea of pigs, the component primarily of following weight portion is made: bone meal 50-100 part, lysozyme 1-10 part, coriolan 1-10 part.

Preferably, in described feed addictive, the weight portion of each component is: bone meal 50 parts, lysozyme 1-4 part, coriolan 1-4 part.

Preferred, in described feed addictive, the weight portion of each component is: bone meal 50 parts, lysozyme 1 part, coriolan 3 parts.

Preferably, described raw material comprises other active components further.

Preferred, other active components described be selected from amino acid, vitamin etc. one or more.

Most preferred, described amino acid is methionine and/or lysine etc., and described vitamin is vitamin A, vitamin C, vitamin E and/or vitamin K etc.

The present invention also provides the application of a kind of above-mentioned feed addictive in the feed of preparation control diarrhea of pigs.

Preferably, in the feed of described 100 weight portions, the feed addictive containing 1-5 % by weight.

The present invention has actively useful effect:

Surprisingly, through repeated tests, the present invention surprisingly finds lysozyme and polysaccharose substance coriolan to combinationally use the effect for preventing and treating diarrhea of pigs and have Synergistic, coordinates other active component results of use better.

Compared with normal diet additive, domestic animal biology feed additive preparation method of the present invention is simple, and compatibility is reasonable, evident in efficacy, can significantly reduce diarrhea of pigs rate, and experiment proves there is special efficacy to improving diarrhea of pigs; Due in additive without western medicine composition, therefore feed addictive has no side effect, and has no drug resistance, pollution-free.

Detailed description of the invention

Below in conjunction with embodiment, the present invention is further described, but embodiments of the present invention are not limited thereto.The experimental technique used in following embodiment if no special instructions, is conventional method.

The extraction of embodiment 1, coriolan

Get the water mixing that rainbow conk dry product adds 50 times of weight, 80 DEG C of lixiviates 4 hours, get supernatant Vacuum Concentration, with 95% alcohol settling of 5 times of concentrate volumes, sediment is dissolved in suitable quantity of water again, takes off albumen by Sevag method, appropriate 95% alcohol settling, vacuum drying obtains coriolan.

The preparation of embodiment 2, feed addictive

Get bone meal 50 weight portion, lysozyme 5 weight portion, makes feed addictive A1 after mixing.

Get bone meal 50 weight portion, coriolan 5 weight portion that embodiment 1 prepares, makes feed addictive A2 after mixing.

Get bone meal 50 weight portion, lysozyme 4 weight portion, coriolan 1 weight portion that embodiment 1 prepares, after mixing, make the feed addictive A3 that lysozyme and coriolan weight ratio are 4:1.

Get bone meal 50 weight portion, lysozyme 2.5 weight portion, coriolan 2.5 weight portion that embodiment 1 prepares, after mixing, make the feed addictive A4 that lysozyme and coriolan weight ratio are 1:1.

Get bone meal 50 weight portion, lysozyme 1.25 weight portion, coriolan 3.75 weight portion that embodiment 1 prepares, after mixing, make the feed addictive A5 that lysozyme and coriolan weight ratio are 1:3.

Get bone meal 50 weight portion, lysozyme 1 weight portion, coriolan 4 weight portion that embodiment 1 prepares, after mixing, make the feed addictive A6 that lysozyme and coriolan weight ratio are 1:4.

The lysozyme used in above-described embodiment adopts gel method, makes the particle of diameter 0.5mm, be lysozyme bag by product through freeze drying.

The application in diarrhea of pigs prevented and treated by embodiment 3, the feed addictive comprising lysozyme and coriolan

For verifying effect of the present invention, feed addictive of the present invention is tested in healthy, that body weight is close porkling.Pig is divided into experimental group (A1-A6) and control group, often organizing is 50.Wherein, the daily ration of experimental group adds above-mentioned feed addictive according to 1% of feed gross weight, the daily ration of control group is the pig feed of the identical weight of not adding above-mentioned feed addictive, each group of pig is thrown something and fed 3 times every day, free choice feeding in experimental period, sufficient drinking-water is provided simultaneously, other every daily management measures are carried out according to the Routine Management of plant, regularly carry out immunity and expelling parasite, the incidence of pig in experimental group and control group in the statistical test phase after 30 days, and calculate the diarrhea of pigs rate (see table 1) of each group.

Table 1

	Diarrhoea head number	Diarrhea rate
			Control group	18	36％
Lysozyme group	15	30％
			Coriolan group	12	24％
Lysozyme+coriolan group (4:1)	8	16％
			Lysozyme+coriolan group (1:1)	6	12％
Lysozyme+coriolan group (1:3)	1	2％
			Lysozyme+coriolan group (1:4)	4	8％

As can be seen from the contrast experiment of embodiment 3, feed addictive of the present invention effectively can prevent and treat diarrhea of pigs, strengthens resistance to infection, reduces the diarrhea rate of pig, particularly lysozyme and combinationally using of coriolan create synergy, decrease the use amount of medicine.When active component total amount remains unchanged, the Be very effective that feed addictive of the present invention is comparatively used alone the feed addictive control diarrhea of pigs of lysozyme or coriolan improves, diarrhea of pigs rate at least reduces 8%, wherein lysozyme and coriolan weight ratio are that the feed addictive effect of 1:3 is the most outstanding, diarrhea of pigs rate is reduced 22%, creates the excellent effect being difficult to expect.

Claims (8)

1. for preventing and treating a feed addictive for diarrhea of pigs, it is characterized in that, the raw material primarily of following weight portion is prepared from: bone meal 50-100 part, lysozyme 1-10 part, coriolan 1-10 part.

2. feed addictive according to claim 1, is characterized in that, described parts by weight of raw materials is bone meal 50-75 part, lysozyme 1-4 part, coriolan 1-4 part.

3. feed addictive according to claim 2, is characterized in that, described parts by weight of raw materials is bone meal 50 parts, lysozyme 1 part, coriolan 3 parts.

4. feed addictive according to claim 1, is characterized in that, described raw material comprises other active components further.

5. feed addictive according to claim 4, is characterized in that, other active components described be selected from amino acid, vitamin etc. one or more.

6. feed addictive according to claim 5, is characterized in that, described amino acid is methionine and/or lysine etc., and described vitamin is vitamin A, vitamin C, vitamin E and/or vitamin K etc.

7. the application of the feed addictive described in any one of claim 1-6 in the feed of preparation control diarrhea of pigs.

8. application according to claim 7, is characterized in that, in the feed of described 100 weight portions, and the feed addictive containing 1-5 % by weight.