CN114642129A - Callicarpa nudiflora cuttage planting method - Google Patents
Callicarpa nudiflora cuttage planting method Download PDFInfo
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- CN114642129A CN114642129A CN202210454732.2A CN202210454732A CN114642129A CN 114642129 A CN114642129 A CN 114642129A CN 202210454732 A CN202210454732 A CN 202210454732A CN 114642129 A CN114642129 A CN 114642129A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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- C—CHEMISTRY; METALLURGY
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- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/18—Prepolymers; Macromolecular compounds
- C09K17/32—Prepolymers; Macromolecular compounds of natural origin, e.g. cellulosic materials
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Abstract
The invention belongs to a cultivation method of economic tree species, and particularly relates to a callicarpa nudiflora cuttage planting method. Which comprises the following steps: (1) pruning: selecting 1-2 year old branches as cutting slips, cutting 15-20cm of cutting branches from positions 5-10cm away from the top ends of the branches, wherein each branch is provided with 4-7 buds; (2) cuttage: cutting the upper end and the lower end of a cutting into regular planes, soaking the planes in a branch soaking agent, and then cutting the planes into a substrate seedbed; (3) managing after cuttage: after the transplanting, watering thoroughly, fertilizing once, and transplanting after the seedling is formed; wherein, the branch soaking agent in the step (2) is prepared by adding vanillic acid, indoleacetic acid and peppermint oil into the callicarpa nudiflora extracting solution and mixing; the concentrations of vanillic acid, indolebutyric acid and peppermint oil are respectively 10-20%, 1-5% and 0.3-0.7%. The callicarpa nudiflora treated by the method has the advantages of short rooting time, high rooting rate, good survival rate after transplanting and important economic value.
Description
Technical Field
The invention belongs to a cultivation method of economic tree species, and particularly relates to a callicarpa nudiflora cuttage planting method.
Background
Callicarpa nudiflora (academic name: Callicarpa nudiflora hook. et Arn.) is Verbenaceae, and can reach 7 m; old branches have no hair and obvious skin holes, and branches with dense grayish brown color and hairy hair of twigs, petioles and inflorescences. The leaves are oval and oblong in shape and are coated with needles, the surfaces of the leaves are dark green, the leaves become black after being dried, the polyspora rosea is developed, and the bracts are linear or coated with needles; calyx, corolla purple or pink, anther oval, tiny, nearly spherical, red, flowering in 6-8 months, and bearing fruit in 8-12 months.
The callicarpa nudiflora leaf has the effects of stopping bleeding, relieving pain, removing blood stasis and reducing swelling. Treating traumatic hemorrhage, traumatic injury swelling and pain, rheumatic swelling and pain, pulmonary tuberculosis hemoptysis, and gastrointestinal hemorrhage; the medicine is refined into injection, and is a good hemostatic. The dried leaves of Callicarpa nudiflora are black after being crushed, so the material is commonly called Henan Heiyao, which is usually opposite to Yunnan Baiyao. Leaves, branches, stems, flowers, fruits and underground roots of callicarpa nudiflora can be used as the medicine, and as the leaves are rich in a plurality of pharmacodynamic active ingredients such as flavone, phenolic acid, saccharides and the like, the medicine has the effects of stopping bleeding, diminishing inflammation, dispelling wind and eliminating dampness, resisting oxidation, resisting tumors and the like, and can be used for treating traumatic bleeding, acne, hepatitis, suppurative inflammation and the like. In addition, the callicarpa nudiflora has good effects on improving liver and kidney functions, preventing and treating gynecological diseases and the like. The beautyberry medicinal material has rich wild resources and long civil history, and is prepared into various Chinese patent medicines such as beautyberry tablets, beautyberry capsules, beautyberry suppositories and the like.
In recent years, the medicinal value of beautyberry plants is continuously increased, the related pharmaceutical industry is rapidly developed, the market demand is more and more large, and the research and development of beautyberry plants are also concerned. For a long time, the research content of the medicinal materials has been focused on clinical application, pathology and chemical component analysis, and how to improve the yield and quality of the medicinal materials by a cultivation method is still in a groping stage.
Chinese patent application CN104663191A discloses a method for breeding callicarpa nudiflora, which comprises selecting annual semi-lignified callicarpa nudiflora branch, cutting in 7-8 months, cutting into 8-10cm cuttings, soaking with 0.1-0.3mg/BRs solution for 12-24h before cutting, wherein the cutting density is 5cm x 10cm, and refining the seedling after 2 months of cutting. Cultivating until 3 months of the next year, and transplanting. The method can improve the rooting rate and the survival rate of the callicarpa nudiflora bracket-inserted seedlings, realizes large-scale quick propagation, ensures that the seedlings grow quickly, can grow to about 1.2m in the same year as transplanting, has low seedling raising cost and high labor efficiency, ensures that the rooting rate of cuttage reaches 85.3 percent, the survival rate of transplanting reaches 91.52 percent, and can better meet the requirements of production and market.
The Chinese patent application CN201410438391.5 discloses a method for rapidly propagating callicarpa nudiflora, which comprises the following steps: (1) preparing spike seedlings; (2) preparing a mixed matrix; (3) preparing cutting slips and cutting; (4) and (5) later-period management and protection. The invention can effectively shorten the time needed by rooting, obviously improve the rooting rate, the rooting number and the maximum root length, and reduce the partial rooting rate; the seedling rate and the transplanting survival rate of the produced nursery stock are high.
The researches explore the cuttage rooting rate and the transplanting survival rate of the callicarpa nudiflora, and obtain certain effect, but the rooting period and the rooting uniformity are not improved, the breeding period is long, the time consumption is long, and the rooting rate and the transplanting survival rate are to be further improved.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a cuttage propagation technology with high rate of callicarpa nudiflora rooting, short rooting period, uniform rooting and developed root system.
In order to realize the purpose of the invention, the technical scheme is as follows:
a callicarpa nudiflora cutting planting method comprises the following steps:
(1) pruning: selecting 1-2 year old branches as cutting slips, cutting 15-20cm of cutting branches from positions 5-10cm away from the top ends of the branches, wherein each branch is provided with 4-7 buds;
(2) cuttage: cutting the upper end and the lower end of a cutting into regular planes, soaking the planes in a branch soaking agent, and then cutting the planes into a substrate seedbed;
(3) managing after cuttage: after the seedlings are inserted, watering, transplanting after the seedlings are grown, and fertilizing;
wherein, the branch soaking agent in the step (2) is prepared by adding vanillic acid, indoleacetic acid and peppermint oil into the callicarpa nudiflora extracting solution and mixing.
Preferably, the concentration of vanillic acid, indoleacetic acid and peppermint oil is 10-20%, 1-5% and 0.3-0.7%, respectively.
Preferably, the callicarpa nudiflora extract in the step (2) is a concentrated solution obtained by extracting 50-75% ethanol, the relative density of the concentrated solution is 1.2 +/-0.1, and the soaking is performed for 1-2h at 10-20 ℃ and then for 1-5h at 25-35 ℃.
Preferably, the matrix in step (2) consists of a base matrix and a nutritional composition in a mass ratio of 1: 0.2-0.5.
Preferably, the basic matrix in step (2) is selected from more than two of fermented traditional Chinese medicine dregs, silkworm excrement, faeces Vespertilionis and fine soil.
Preferably, in step (2) the base matrix is prepared by mixing, by mass, 0.5-1: 0.1-0.5: 0.1-0.3:1 of fermented traditional Chinese medicine dregs, silkworm excrement, faeces Vespertilionis and fine soil; silkworm excrement and faeces Vespertilionis need to be pulverized in advance.
Preferably, the medicine dregs are prepared from the following components in percentage by mass of 1: 2: 0.3:1, naturally fermenting the dregs of the decoction after the callicarpa nudiflora leaf, the senecio scandens and the eucommia ulmoides leaf are extracted for 2-4 months.
Preferably, the extraction is water extraction or alcohol extraction.
Preferably, the nutritional composition comprises indoleacetic acid, glutamic acid, vitamin B2, sodium alginate and hypromellose.
Preferably, the mass ratio of the indoleacetic acid to the glutamic acid to the vitamin B2 to the sodium alginate to the hypromellose is 1:1-5:0.3-0.6:0.5-0.8: 0.2-0.5.
Preferably, the preparation method of the nutrient comprises: mixing sodium alginate, 50-70% indoleacetic acid, glutamic acid and vitamin B2, adding 70-75% ethanol, granulating by wet method, oven drying, adding hypromellose and the rest indoleacetic acid, adding 85% ethanol, mixing, and oven drying.
Preferably, the top buds of the selected cuttings are subtracted 6-10 days before pruning in the step (1); the row spacing of the cuttage plants in the step (2) is 15cm multiplied by 25 cm.
Preferably, the fertilizer applied in the step (3) consists of cow dung, human excrement, kelp powder and duck egg shell powder in a mass ratio of 50-60:30-40:5-10: 0.5-1.
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention mixes the nutrition combination containing rooting liquid, glutamic acid and vitamin B2, adds sodium alginate with proper proportion to prepare nutrition particles, wraps a certain amount of indoleacetic acid and hydroxypropyl methylcellulose outside the nutrition particles, mixes the nutrition particles into the cuttage substrate, after watering, the hydroxypropyl methylcellulose swells firstly, slowly releases the indoleacetic acid in the substrate into the soil, not only greatly improves the moisture retention of the soil, but also plays a role in uniform and mild rooting for callicarpa nudiflora cuttings, then the sodium alginate swells inside the nutrition particles, slowly releases the rest indoleacetic acid and nutrients, promotes the growth and breeding of the root system.
(2) The branch soaking agent is added with the vanillic acid and the mint oil, so that the good sterilization effect is achieved, the surface of a root system can be activated, the rooting of the cutting branches is promoted, and the rooting time is shortened.
(3) According to the invention, fermented traditional Chinese medicine dregs, silkworm excrement and faeces Vespertilionis are added into the seedbed substrate for cuttage of callicarpa nudiflora branches, the substrate is loose and fertile, the rooting effect can be obviously promoted, the rooting rate is high, the root system is developed, the survival rate after transplantation is good, and the seedling raising method is superior to that of the traditional cuttage propagation seedling raising.
Detailed Description
The present invention will be further described with reference to the following embodiments.
The fermented traditional Chinese medicine dregs in the following examples are prepared by mixing the following raw materials in a mass ratio of 1: 2: 0.3:1, the dregs of the decoction after the callicarpa nudiflora leaf, the senecio scandens and the eucommia ulmoides leaf are extracted are fermented by natural stacking in a field for three months.
Example 1
The cutting planting method of callicarpa nudiflora of the embodiment comprises the following steps:
(1) pruning: selecting 1-year-old branches as cutting slips, cutting off top buds of the selected cutting branches 6 days before pruning, cutting off 15cm cutting branches from 5cm away from the tops of the branches, wherein each branch is provided with 4 buds;
(2) cuttage: cutting the upper and lower ends of the cutting into regular planes, soaking in a branch soaking agent at 20 deg.C for 2 hr, and then soaking at 25-35 deg.C for 4 hr. Taking out, and cutting into a specific seedbed matrix with the cutting depth of 5 cm;
A. the preparation of the branch soaking agent is as follows:
1) extracting folium Callicarpae Formosanae with 5 times of 75% ethanol under heating and refluxing, and concentrating under reduced pressure at 60 deg.C to relative density of 1.2 to obtain folium Callicarpae Formosanae extractive solution;
2) adding appropriate vanillic acid, indoleacetic acid and peppermint oil into the Callicarpa nudiflora extractive solution to make the concentrations of the vanillic acid, the indoleacetic acid and the peppermint oil respectively 20%, 1% and 0.3%, and mixing uniformly to obtain the branch soaking agent.
B. The matrix is prepared as follows:
1) simply crushing the silkworm excrement and the faeces bombycis, and mixing the fermented traditional Chinese medicine dregs, the silkworm excrement, the faeces bombycis and the fine soil according to a mass ratio of 0.5: 0.1: mixing and stirring at a ratio of 0.1:1 to obtain a basic matrix;
2) mixing sodium alginate, 50% indoleacetic acid, glutamic acid and vitamin B2, adding 75% ethanol, granulating by wet method, oven drying, adding hypromellose and the rest indoleacetic acid, adding 85% ethanol, mixing, and oven drying to obtain nutritional granule. Wherein the mass ratio of the indoleacetic acid to the glutamic acid to the vitamin B2 to the sodium alginate to the hydroxypropyl methylcellulose is 1:1:0.3:0.5: 0.2.
3) And uniformly mixing and stirring the basic matrix and the nutrient particles according to the mass ratio of 1:0.2 to obtain the seedbed matrix.
(3) Managing after cuttage: watering once after cuttage, and selecting proper time for transplanting after seedling formation.
Example 2
The cutting planting method of callicarpa nudiflora of the embodiment comprises the following steps:
(1) pruning: selecting branches growing for 2 years as cutting slips, cutting off top buds of the selected cutting branches 8 days before pruning, cutting off 20cm cutting branches from the position 8cm away from the top ends of the branches, wherein each branch is provided with 6 buds;
(2) cuttage: cutting the upper and lower ends of the cutting into regular planes, soaking in a branch soaking agent at 15 deg.C for 2 hr, and then soaking at 30 deg.C for 4 hr. Taking out, and cutting into specific seedbed matrix;
A. the preparation of the branch soaking agent is as follows:
1) extracting folium Callicarpae Formosanae with 5 times of 70% ethanol under reflux, and concentrating at 60 deg.C under reduced pressure to relative density of 1.2 to obtain folium Callicarpae Formosanae extractive solution;
2) adding appropriate vanillic acid, indoleacetic acid and peppermint oil into the Callicarpa nudiflora extractive solution to make the concentrations of 15%, 3% and 0.5%, respectively, and mixing to obtain the final product.
B. The matrix is prepared as follows:
1) simply crushing the silkworm excrement and the faeces bombycis, and mixing the fermented traditional Chinese medicine dregs, the silkworm excrement, the faeces bombycis and the fine soil according to a mass ratio of 1:0.5: mixing and stirring at a ratio of 0.3:1 to obtain a basic matrix;
2) mixing sodium alginate, 60% indoleacetic acid, glutamic acid and vitamin B2, adding 70% ethanol, granulating by wet method, oven drying, adding hypromellose and the rest indoleacetic acid, adding 85% ethanol, mixing, and oven drying to obtain nutritional granule. Wherein the mass ratio of the indoleacetic acid to the glutamic acid to the vitamin B2 to the sodium alginate to the hydroxypropyl methylcellulose is 1:5:0.6:0.8: 0.5.
3) And uniformly mixing and stirring the basic matrix and the nutritional particles according to the mass ratio of 1:0.5 to obtain the seedbed matrix.
(3) Managing after cuttage: watering once after cuttage, and selecting proper time for transplanting after seedling formation.
Example 3
The cutting planting method of callicarpa nudiflora of the embodiment comprises the following steps:
(1) pruning: selecting 1-year-old branches as cutting slips, cutting off top buds of the selected cutting branches 6 days before pruning, cutting off 20cm cutting branches from a position 10cm away from the top ends of the branches, and arranging 5 buds on each branch;
(2) cuttage: cutting the upper and lower ends of the cutting into regular planes, soaking in a branch soaking agent at 15 deg.C for 1 hr, and soaking at 25 deg.C for 2 hr. Taking out, and cutting into specific seedbed matrix;
A. the preparation of the branch soaking agent is as follows:
1) extracting folium Callicarpae Formosanae with 5 times of 75% ethanol under heating and refluxing, and concentrating under reduced pressure at 60 deg.C to relative density of 1.2 to obtain folium Callicarpae Formosanae extractive solution;
2) adding appropriate vanillic acid, indoleacetic acid and peppermint oil into the Callicarpa nudiflora extractive solution to make the concentrations of 15%, 3% and 0.3%, respectively, and mixing to obtain the final product.
B. The matrix is prepared as follows:
1) simply crushing the silkworm excrement and the faeces bombycis, and mixing the fermented traditional Chinese medicine dregs, the silkworm excrement, the faeces bombycis and the fine soil according to a mass ratio of 0.8: 0.2: mixing and stirring at a ratio of 0.2:1 to obtain a basic matrix;
2) mixing sodium alginate, 60% indoleacetic acid, glutamic acid and vitamin B2, adding 75% ethanol, granulating by wet method, oven drying, adding hypromellose and the rest indoleacetic acid, adding 85% ethanol, mixing, and oven drying to obtain nutritional granule. Wherein the mass ratio of the indoleacetic acid to the glutamic acid to the vitamin B2 to the sodium alginate to the hydroxypropyl methylcellulose is 1:3:0.4:0.6: 0.3.
3) And uniformly mixing and stirring the basic matrix and the nutrient particles according to the mass ratio of 1:0.3 to obtain the seedbed matrix.
(3) Managing after cuttage: watering once after cuttage, and selecting proper time for transplanting after seedling formation.
Comparative example 1
The comparative example is different from the example 1 in that vanillic acid is removed, citric acid with the same concentration is added, and the rest is subjected to cuttage breeding according to the method in the example 1.
Comparative example 2
The difference between the comparative example and the example 1 is that the raw material composition of the nutritional composition is different, and the mass ratio of the indoleacetic acid, the glutamic acid, the vitamin B2, the sodium alginate and the hypromellose is 1:0.5:1:1: 0.1. The rest is cut and bred according to the method of the embodiment 1.
Comparative example 3
The difference between the comparative example and the example 1 is that the preparation method of the nutritional composition is different, and the specific steps are mixing sodium alginate, indoleacetic acid, glutamic acid, vitamin B2 and hydroxypropyl methylcellulose, adding 85% ethanol, mixing, performing wet granulation, and drying to obtain the nutritional composition.
Comparative example 4
The difference between the comparative example and the example 1 is that the basic matrix does not contain silkworm excrement and faeces Vespertilionis, the dosage is supplemented by fermented traditional Chinese medicine dregs, and the rest is subjected to cuttage propagation according to the method in the example 1.
Test 1
30 cuttings are respectively inserted according to the methods of the above examples and comparative examples, a small arch shed is built, the rooting propagation conditions of the callicarpa nudiflora cutting branches of the examples 1-3 and the comparative examples 1-4 are observed, the number of rooting plants in 10 days is counted, the root system conditions of the cuttings are checked, and the results are shown in table 1.
TABLE 1 Callicarpa nudiflora cutting propagation technique rooting conditions
| Grouping | Rooting cuttings/plants | Average longest root length per plant/cm |
| Example 1 | 30 | 23 |
| Example 2 | 30 | 22 |
| Example 3 | 29 | 23 |
| Comparative example 1 | 27 | 19 |
| Comparative example 2 | 26 | 21 |
| Comparative example 3 | 26 | 20 |
| Comparative example 4 | 24 | 16 |
Test example 2
50 cuttings are respectively inserted according to the methods of the above embodiments and comparative examples, the rooted cuttings are transplanted in the same period after 2 months, the transplanting period is selected from Jiangxi from late 2 months to 3 months, the transplanting row spacing is 2m, the plant spacing is 1.5m, 5 kg of fertilizer is applied to each plant after transplanting, and the fertilizer is prepared by mixing the following components in a mass ratio of 55: 35: 8:1, compounding cow dung, human excrement, kelp powder and duck egg shell powder, and watering properly; the survival of the plants was counted and the results are shown in Table 2. Survival% — number of surviving plants/rooted cuttings + 100%.
TABLE 2 influence of Callicarpa nudiflora cutting propagation technique on survival rate of transplantation
| Grouping | Rooting cuttings/plants | Adult plant | The survival rate is high |
| Example 1 | 50 | 50 | 100.0 |
| Example 2 | 50 | 50 | 100.0 |
| Example 3 | 50 | 48 | 96.0 |
| Comparative example 1 | 45 | 42 | 93.3 |
| Comparative example 2 | 48 | 44 | 91.7 |
| Comparative example 3 | 45 | 41 | 91.1 |
| Comparative example 4 | 44 | 38 | 86.4 |
The above detailed description is specific to one possible embodiment of the present invention, and the embodiment is not intended to limit the scope of the present invention, and all equivalent implementations or modifications without departing from the scope of the present invention should be included in the technical scope of the present invention.
Claims (10)
1. A cutting planting method of callicarpa nudiflora is characterized by comprising the following steps:
(1) pruning: selecting 1-2 year-old branches as cutting slips, cutting 15-20cm of cutting slips downwards from a position 5-10cm away from the top ends of the branches, wherein each branch is provided with 4-7 buds;
(2) cuttage: cutting the upper end and the lower end of a cutting into regular planes, soaking the planes in a branch soaking agent, and then cutting the planes into a substrate seedbed;
(3) managing after cuttage: after the seedlings are inserted, watering, transplanting after the seedlings are grown, and fertilizing;
wherein, the branch soaking agent in the step (2) is prepared by adding vanillic acid, indoleacetic acid and peppermint oil into the callicarpa nudiflora extracting solution and mixing.
2. The cutting propagation method according to claim 1, wherein the concentration of vanillic acid, indolylacetic acid and peppermint oil in step (2) is 10-20%, 1-5% and 0.3-0.7%, respectively.
3. The cutting propagation method according to claim 1, wherein the callicarpa nudiflora extract in step (2) is a concentrate of callicarpa nudiflora leaves extracted with 50-75% ethanol, the relative density of the concentrate is 1.2 ± 0.1, the soaking is performed at 10-20 ℃ for 1-2h, and then at 25-35 ℃ for 1-5 h.
4. The cutting propagation method according to claim 1, wherein the substrate in the step (2) is composed of a base substrate and a nutrient composition in a mass ratio of 1:0.2-0.5, and the base substrate is selected from more than two of fermented traditional Chinese medicine dregs, silkworm excrement, faeces Vespertilionis and fine soil.
5. The cutting propagation method according to claim 1, wherein the basic matrix in the step (2) is prepared by mixing the components in a mass ratio of 0.5-1: 0.1-0.5: 0.1-0.3:1, and is prepared by mixing fermented traditional Chinese medicine dregs, silkworm excrement, faeces Vespertilionis and fine soil.
6. The cutting propagation method according to claim 5, wherein the medicine residue is prepared from the following components in a mass ratio of 1: 2: 0.3:1, extracting folium Callicarpae Formosanae, herba Senecionis Scandentis and folium Eucommiae, and naturally fermenting.
7. The cutting propagation method according to claim 4, wherein the nutritional composition in step (2) comprises indoleacetic acid, glutamic acid, vitamin B2, sodium alginate and hypromellose; the mass ratio of the indoleacetic acid to the glutamic acid to the vitamin B2 to the sodium alginate to the hydroxypropyl methylcellulose is 1:1-5:0.3-0.6:0.5-0.8: 0.2-0.5.
8. The cutting propagation method according to claim 7, wherein the preparation method of the nutritional composition comprises: mixing sodium alginate, 50-70% indoleacetic acid, glutamic acid and vitamin B2, adding 70-75% ethanol, granulating by wet method, oven drying, adding hypromellose and the rest indoleacetic acid, adding 85% ethanol, mixing, and oven drying.
9. The cutting propagation method according to claim 1, wherein the apical buds of the selected cuttings are subtracted 6-10 days before pruning in step (1); the row spacing of the cuttage plants in the step (2) is 15cm multiplied by 25 cm.
10. The cutting propagation method according to claim 1, wherein the fertilizer applied in the step (3) consists of cow dung, human excrement, kelp powder and duck egg shell powder in a mass ratio of 50-60:30-40:5-10: 0.5-1.
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