CN114616320B - 转化微生物、以及使用了该微生物的聚羟基烷酸酯的制造方法 - Google Patents

转化微生物、以及使用了该微生物的聚羟基烷酸酯的制造方法 Download PDF

Info

Publication number
CN114616320B
CN114616320B CN202080062790.XA CN202080062790A CN114616320B CN 114616320 B CN114616320 B CN 114616320B CN 202080062790 A CN202080062790 A CN 202080062790A CN 114616320 B CN114616320 B CN 114616320B
Authority
CN
China
Prior art keywords
ala
leu
gly
val
ser
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202080062790.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN114616320A (zh
Inventor
毛利佳弘
有川尚志
佐藤俊辅
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kaneka Corp
Original Assignee
Kaneka Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corp filed Critical Kaneka Corp
Publication of CN114616320A publication Critical patent/CN114616320A/zh
Application granted granted Critical
Publication of CN114616320B publication Critical patent/CN114616320B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202080062790.XA 2019-09-09 2020-08-04 转化微生物、以及使用了该微生物的聚羟基烷酸酯的制造方法 Active CN114616320B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-163743 2019-09-09
JP2019163743 2019-09-09
PCT/JP2020/029763 WO2021049207A1 (ja) 2019-09-09 2020-08-04 形質転換微生物、及び当該微生物を用いたポリヒドロキシアルカン酸の製造方法

Publications (2)

Publication Number Publication Date
CN114616320A CN114616320A (zh) 2022-06-10
CN114616320B true CN114616320B (zh) 2025-02-07

Family

ID=74866473

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080062790.XA Active CN114616320B (zh) 2019-09-09 2020-08-04 转化微生物、以及使用了该微生物的聚羟基烷酸酯的制造方法

Country Status (5)

Country Link
US (1) US12351854B2 (https=)
EP (1) EP4029946A4 (https=)
JP (1) JP7535527B2 (https=)
CN (1) CN114616320B (https=)
WO (1) WO2021049207A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7554177B2 (ja) * 2019-02-28 2024-09-19 株式会社カネカ 形質転換微生物、及びポリヒドロキシアルカン酸の製造方法
WO2023085374A1 (ja) * 2021-11-11 2023-05-19 株式会社カネカ ポリヒドロキシアルカン酸の製造方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105779488A (zh) * 2016-03-23 2016-07-20 清华大学 一种诱导外源基因在革兰氏阴性菌中表达的系统及其应用
WO2016194771A1 (ja) * 2015-05-29 2016-12-08 国立大学法人北海道大学 ｍｔｇＡ遺伝子の欠損した微生物

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007259708A (ja) 2006-03-27 2007-10-11 Kaneka Corp 新規生分解性ポリエステルの製造方法
US7384766B2 (en) 2006-07-26 2008-06-10 Kaneka Corporation Gene-substituted microorganisms, and production method of polyesters using the same
JP7270556B2 (ja) 2018-01-17 2023-05-10 株式会社カネカ 高組成比率の３ｈｈモノマー単位を含む共重合ｐｈａを生産する形質転換微生物およびそれによるｐｈａの製造方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016194771A1 (ja) * 2015-05-29 2016-12-08 国立大学法人北海道大学 ｍｔｇＡ遺伝子の欠損した微生物
CN105779488A (zh) * 2016-03-23 2016-07-20 清华大学 一种诱导外源基因在革兰氏阴性菌中表达的系统及其应用

Also Published As

Publication number Publication date
JP7535527B2 (ja) 2024-08-16
CN114616320A (zh) 2022-06-10
US20220315958A1 (en) 2022-10-06
JPWO2021049207A1 (https=) 2021-03-18
EP4029946A1 (en) 2022-07-20
US12351854B2 (en) 2025-07-08
EP4029946A4 (en) 2023-08-30
WO2021049207A1 (ja) 2021-03-18

Similar Documents

Publication Publication Date Title
JPWO2019142845A1 (ja) 高組成比率の３ｈｈモノマー単位を含む共重合ｐｈａを生産する形質転換微生物およびそれによるｐｈａの製造方法
WO2018021046A1 (ja) ３ｈｈ単位含有共重合ｐｈａを生産する形質転換体、及び当該ｐｈａの製造方法
JP7748499B2 (ja) 形質転換微生物、及びポリヒドロキシアルカン酸の製造方法
JP6313708B2 (ja) 高分子量ｐｈａ生産微生物とそれを用いた高分子量ｐｈａの製造方法
CN114450416B (zh) 聚羟基烷酸酯的制造方法
CN114616320B (zh) 转化微生物、以及使用了该微生物的聚羟基烷酸酯的制造方法
US6872564B1 (en) Method of producing copolymer polyester
JP6853787B2 (ja) スクロース資化性を有するｐｈａ生産微生物、及び該微生物を用いたｐｈａの製造方法
JPWO2005098001A1 (ja) 新規形質転換体
JP2021108581A (ja) 形質転換微生物、及びポリヒドロキシアルカン酸の製造方法
US10876140B2 (en) Method for producing polyhydroxyalkanoic acid, and microbes
WO2024166830A1 (ja) 形質転換微生物、及びポリヒドロキシアルカン酸の製造方法
JP7425783B2 (ja) 形質転換微生物、及びポリヒドロキシアルカン酸の製造方法
JP2008086238A (ja) ポリヒドロキシアルカノエートの製造方法
CN120659867A (zh) 转化微生物及聚羟基烷酸酯的制造方法
JP2009225775A (ja) ポリヒドロキシアルカン酸の製造方法
KR20220004130A (ko) 고분자량 코폴리머를 합성하기 위한 유전자

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant