JP7535527B2 - 形質転換微生物、及び当該微生物を用いたポリヒドロキシアルカン酸の製造方法 - Google Patents

形質転換微生物、及び当該微生物を用いたポリヒドロキシアルカン酸の製造方法 Download PDF

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JP7535527B2
JP7535527B2 JP2021545163A JP2021545163A JP7535527B2 JP 7535527 B2 JP7535527 B2 JP 7535527B2 JP 2021545163 A JP2021545163 A JP 2021545163A JP 2021545163 A JP2021545163 A JP 2021545163A JP 7535527 B2 JP7535527 B2 JP 7535527B2
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amino acid
expression
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JPWO2021049207A1 (https=
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佳弘 毛利
尚志 有川
俊輔 佐藤
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Kaneka Corp
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2021545163A 2019-09-09 2020-08-04 形質転換微生物、及び当該微生物を用いたポリヒドロキシアルカン酸の製造方法 Active JP7535527B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019163743 2019-09-09
JP2019163743 2019-09-09
PCT/JP2020/029763 WO2021049207A1 (ja) 2019-09-09 2020-08-04 形質転換微生物、及び当該微生物を用いたポリヒドロキシアルカン酸の製造方法

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JPWO2021049207A1 JPWO2021049207A1 (https=) 2021-03-18
JP7535527B2 true JP7535527B2 (ja) 2024-08-16

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US (1) US12351854B2 (https=)
EP (1) EP4029946A4 (https=)
JP (1) JP7535527B2 (https=)
CN (1) CN114616320B (https=)
WO (1) WO2021049207A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7554177B2 (ja) * 2019-02-28 2024-09-19 株式会社カネカ 形質転換微生物、及びポリヒドロキシアルカン酸の製造方法
WO2023085374A1 (ja) * 2021-11-11 2023-05-19 株式会社カネカ ポリヒドロキシアルカン酸の製造方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105779488A (zh) 2016-03-23 2016-07-20 清华大学 一种诱导外源基因在革兰氏阴性菌中表达的系统及其应用
WO2016194771A1 (ja) 2015-05-29 2016-12-08 国立大学法人北海道大学 ｍｔｇＡ遺伝子の欠損した微生物
WO2019142845A1 (ja) 2018-01-17 2019-07-25 株式会社カネカ 高組成比率の３ｈｈモノマー単位を含む共重合ｐｈａを生産する形質転換微生物およびそれによるｐｈａの製造方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007259708A (ja) 2006-03-27 2007-10-11 Kaneka Corp 新規生分解性ポリエステルの製造方法
US7384766B2 (en) 2006-07-26 2008-06-10 Kaneka Corporation Gene-substituted microorganisms, and production method of polyesters using the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016194771A1 (ja) 2015-05-29 2016-12-08 国立大学法人北海道大学 ｍｔｇＡ遺伝子の欠損した微生物
CN105779488A (zh) 2016-03-23 2016-07-20 清华大学 一种诱导外源基因在革兰氏阴性菌中表达的系统及其应用
WO2019142845A1 (ja) 2018-01-17 2019-07-25 株式会社カネカ 高組成比率の３ｈｈモノマー単位を含む共重合ｐｈａを生産する形質転換微生物およびそれによるｐｈａの製造方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
SHEN, Rui et al.，Manipulation of polyhydroxyalkanoate granular sizes in Halomonas bluephagenesis，Metablic Engineering，2019年04月06日，Vol.54，p.117-126
UniProtKB，Accesion No.Q0K924, H16_A2405 gene，［online］, Last sequence update: October 3, 2006, ［retrived on 2020.08.25］, 〈URL:https://www.uniprot.org/uniprot/Q0K924〉
UniProtKB，Accesion No.Q0KBU9, H16_A1386 gene，［online］, Last sequence update: October 3, 2006, ［retrived on 2020.08.25］, 〈URL:https://www.uniprot.org/uniprot/Q0KBU9〉
ZHANG, Xing-Chen et al.，Engineering cell wall synthesis mechanism for enhanced PHB accumulation in E.coli，Metabolic Engineering，2017年11月24日，Vol.45，p.32-42

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Publication number Publication date
CN114616320A (zh) 2022-06-10
US20220315958A1 (en) 2022-10-06
JPWO2021049207A1 (https=) 2021-03-18
EP4029946A1 (en) 2022-07-20
US12351854B2 (en) 2025-07-08
EP4029946A4 (en) 2023-08-30
WO2021049207A1 (ja) 2021-03-18
CN114616320B (zh) 2025-02-07

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