CN114585736A - 一种嘌呤霉素连接子及其在体外核酸展示肽合成中的应用 - Google Patents

一种嘌呤霉素连接子及其在体外核酸展示肽合成中的应用 Download PDF

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CN114585736A
CN114585736A CN202080066305.6A CN202080066305A CN114585736A CN 114585736 A CN114585736 A CN 114585736A CN 202080066305 A CN202080066305 A CN 202080066305A CN 114585736 A CN114585736 A CN 114585736A
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mrna
sequence
nucleotides
puromycin
puromycin linker
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CN114585736B (zh
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桑国芹
焦少灼
谢莹莹
徐猛
李宗文
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Beijing Xunyin Biological Technology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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Abstract

提供了一种嘌呤霉素连接子及其在体外核酸展示肽合成中的应用。所述嘌呤霉素连接子为含修饰无分支的单链DNA，该单链DNA包括第一段核苷酸和第二段核苷酸；所述第一段核苷酸的5'端和所述第二段核苷酸的5'端相连接形成包含两个3’末端的连接体结构；所述第一段核苷酸的3’端修饰有嘌呤霉素；所述第二段核苷酸按照5’至3’的顺序包括mRNA连接位点和逆转录位点。所述嘌呤霉素连接子合成方法简单，效率高达53％，极大地提高了关键原料获取的效率，进一步提高系统效率及缩减了cDNA‑蛋白的合成成本。

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PCT国内申请，说明书已公开。

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  1. PCT国内申请，权利要求书已公开。
CN202080066305.6A 2020-10-27 2020-10-27 一种嘌呤霉素连接子及其在体外核酸展示肽合成中的应用 Active CN114585736B (zh)

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PCT/CN2020/123835 WO2022087804A1 (zh) 2020-10-27 2020-10-27 一种嘌呤霉素连接子及其在体外核酸展示肽合成中的应用

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007046520A1 (ja) * 2005-10-18 2007-04-26 National Institute Of Advanced Industrial Science And Technology 固定化ピューロマイシン・リンカーを用いたタンパク質のスクリーニング方法
CN102227638A (zh) * 2008-09-30 2011-10-26 雅培制药有限公司 Rna展示的改良方法
US20160076022A1 (en) * 2013-03-13 2016-03-17 The University Of Tokyo Nucleic acid linker

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104774923B (zh) * 2015-03-11 2017-10-27 华中农业大学 一种测定转录调控复合体的方法
JP6506838B2 (ja) * 2015-03-31 2019-05-08 国立大学法人埼玉大学 試験管内淘汰及び分子間相互作用解析用高速光架橋型共用リンカー、そのリンカーを用いた試験管内淘汰方法
ES2929650T3 (es) * 2016-03-30 2022-11-30 Epsilon Molecular Eng Inc Método para el cribado in vitro de alta velocidad

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007046520A1 (ja) * 2005-10-18 2007-04-26 National Institute Of Advanced Industrial Science And Technology 固定化ピューロマイシン・リンカーを用いたタンパク質のスクリーニング方法
CN102227638A (zh) * 2008-09-30 2011-10-26 雅培制药有限公司 Rna展示的改良方法
US20160076022A1 (en) * 2013-03-13 2016-03-17 The University Of Tokyo Nucleic acid linker

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WO2022087804A1 (zh) 2022-05-05

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