CN114574395A - Bacillus megaterium and biofertilizer capable of antagonizing colletotrichum gloeosporioides - Google Patents
Bacillus megaterium and biofertilizer capable of antagonizing colletotrichum gloeosporioides Download PDFInfo
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- CN114574395A CN114574395A CN202210264928.5A CN202210264928A CN114574395A CN 114574395 A CN114574395 A CN 114574395A CN 202210264928 A CN202210264928 A CN 202210264928A CN 114574395 A CN114574395 A CN 114574395A
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- 241000194107 Bacillus megaterium Species 0.000 title claims abstract description 37
- 241001529387 Colletotrichum gloeosporioides Species 0.000 title claims description 9
- 230000003042 antagnostic effect Effects 0.000 title description 8
- 229960000892 attapulgite Drugs 0.000 claims abstract description 42
- 229910052625 palygorskite Inorganic materials 0.000 claims abstract description 42
- 239000003337 fertilizer Substances 0.000 claims abstract description 35
- 241000209094 Oryza Species 0.000 claims abstract description 25
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 25
- 235000009566 rice Nutrition 0.000 claims abstract description 25
- 241000894006 Bacteria Species 0.000 claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 239000002270 dispersing agent Substances 0.000 claims abstract description 22
- 239000002994 raw material Substances 0.000 claims abstract description 16
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 12
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 12
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 12
- 239000000661 sodium alginate Substances 0.000 claims abstract description 12
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 12
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 12
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 11
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 11
- 238000001354 calcination Methods 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 14
- 239000003610 charcoal Substances 0.000 claims description 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 10
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 10
- 239000002202 Polyethylene glycol Substances 0.000 claims description 10
- 230000003213 activating effect Effects 0.000 claims description 10
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 10
- 229920001223 polyethylene glycol Polymers 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 239000011790 ferrous sulphate Substances 0.000 claims description 5
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000001509 sodium citrate Substances 0.000 claims description 5
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 238000001132 ultrasonic dispersion Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 238000002715 modification method Methods 0.000 claims description 4
- 239000010903 husk Substances 0.000 claims 3
- 230000000694 effects Effects 0.000 abstract description 9
- 241000196324 Embryophyta Species 0.000 abstract description 7
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 4
- 239000011574 phosphorus Substances 0.000 abstract description 4
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 4
- 230000008635 plant growth Effects 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 239000002956 ash Substances 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000004090 dissolution Methods 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract description 2
- 150000007524 organic acids Chemical class 0.000 abstract description 2
- 239000002689 soil Substances 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000002068 microbial inoculum Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000002485 combustion reaction Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000010304 firing Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a biological fertilizer capable of effectively preventing and controlling anthracnose, which comprises the following raw materials in parts by weight: 10-30 parts of bacillus megatherium, 5-10 parts of rice hull ash, 1-6 parts of monopotassium phosphate, 1-6 parts of modified attapulgite and 5-10 parts of sodium alginate. The biological fertilizer for preventing and controlling anthracnose of the invention takes bacillus megaterium as a main active ingredient and takes rice hull ash, monopotassium phosphate, sodium alginate and modified attapulgite as synergistic ingredients. The bacillus megaterium is activated by the viable bacteria liquid, the activated liquid bacteria liquid can secrete organic acid, can effectively accelerate the dissolution of inorganic phosphorus in soil, improves the absorption and utilization of phosphorus by plants, can improve the plant growth efficiency when applied to a fertilizer, and can effectively prevent and control the occurrence of plant anthracnose; the attapulgite is modified and calcined, the activity is enhanced, and the dispersing capacity of the raw materials can be improved after the treatment of the ultrasonic dispersing agent; meanwhile, the added rice hull ash has high specific surface area, enhances the matching effect among raw materials, promotes the performance of the raw material products to be stronger, further improves the fertilizer effect, is beneficial to the production of biological fertilizer and growth-promoting microbial inoculum, and can be further developed and developed.
Description
Technical Field
The invention relates to the technical field of bacillus megaterium, in particular to bacillus megaterium and a biological fertilizer capable of antagonizing colletotrichum gloeosporioides.
Background
A biological fertilizer in the narrow sense is a product which can make agricultural and forestry crops obtain specific fertilizer effect by means of microbial life activity, and is also called as an inoculant or bacterial fertilizer, and does not contain nutrient elements per se, so that the biological fertilizer cannot replace chemical fertilizers. The biological fertilizer is a product containing both nutrient elements required by crops and microorganisms, is a combination of organisms, organic matters and inorganic matters, and can replace chemical fertilizers to provide various nutrient elements required by the growth and development of agricultural and forestry crops. The application of a large amount of chemical fertilizers and pesticides has the advantages and disadvantages for human beings, advocates 'ecological forestry and organic agriculture' for promoting interest and removing disadvantages, gradually realizes the purpose of preventing and treating plant diseases and insect pests by using organic biofertilizers and adopting a microbial method without using chemical fertilizers or less medicaments.
The prior biological fertilizer has poor fertilizer efficiency, has no obvious inhibition effect on the occurrence of anthracnose of forest plants, and simultaneously has poor effect of applying the common bacillus megaterium to the biological fertilizer, so further improvement and treatment are needed.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide bacillus megaterium and a biological fertilizer capable of antagonizing colletotrichum gloeosporioides.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the invention provides a bacillus megaterium capable of antagonizing colletotrichum gloeosporioides, which is obtained by activating bacillus megaterium with viable bacteria liquid:
the specific activating treatment method comprises the following steps:
step one, preparation of viable bacteria liquid: mixing 10-20 parts of sucrose, 5-10 parts of ammonium sulfate and 1-5 parts of iron sulfate heptahydrate, then adding 1-5 parts of ferrous sulfate, and then adjusting the pH value to 7.5-7.8 to obtain viable bacteria liquid;
and step two, feeding the bacillus megaterium into live bacteria liquid for culturing, wherein the culture temperature is 26-30 ℃, and finishing the culture.
A biological fertilizer comprises the following raw materials in parts by weight:
10-30 parts of bacillus megatherium, 5-10 parts of rice hull ash, 1-6 parts of monopotassium phosphate, 1-6 parts of modified attapulgite and 5-10 parts of sodium alginate.
Preferably, the biological fertilizer comprises the following raw materials in parts by weight:
20-30 parts of bacillus megatherium, 7-10 parts of rice hull ash, 3-5 parts of monopotassium phosphate, 2-5 parts of modified attapulgite and 6-9 parts of sodium alginate.
Preferably, the biological fertilizer comprises the following raw materials in parts by weight:
20 parts of bacillus megatherium, 7.5 parts of rice hull ash, 3.5 parts of monopotassium phosphate, 3.5 parts of modified attapulgite and 7.5 parts of sodium alginate.
Preferably, the method for modifying the modified attapulgite comprises the following steps: calcining the attapulgite for 10-20min at the calcining temperature of 500-600 ℃, cooling to 60 ℃ after the calcining is finished, then sending the attapulgite into a dispersant for ultrasonic dispersion for 10-20min at the ultrasonic power of 500-700W, then taking out the attapulgite, washing and drying the attapulgite to obtain the modified attapulgite.
Preferably, the preparation method of the dispersant is as follows: the preparation method of the dispersant comprises the following steps: adding polyethylene glycol into an acetone solvent according to the weight ratio of 1:3, then adding sodium citrate accounting for 10-20% of the total weight of the polyethylene glycol, then adding phosphoric acid, and adjusting the pH value to 6.0 to obtain the dispersing agent.
Preferably, the rice hull ash is obtained by performing charcoal combustion on rice hulls at the charcoal combustion temperature of 1000-1500 ℃, and then performing proton irradiation treatment.
Preferably, the power of the proton irradiation treatment is 500-700W, and the irradiation time is 25-35 min.
Preferably, the power of the proton irradiation treatment is 600W, and the irradiation time is 30 min.
Preferably, the charcoal firing temperature is 1000-.
Compared with the prior art, the invention has the following beneficial effects:
the biological fertilizer for preventing and controlling anthracnose of the invention takes bacillus megaterium as a main active ingredient and takes rice hull ash, monopotassium phosphate, sodium alginate and modified attapulgite as synergistic ingredients. The bacillus megaterium is activated by the viable bacteria liquid, the activated liquid bacteria liquid can secrete organic acid, can effectively accelerate the dissolution of inorganic phosphorus in soil, improve the absorption and utilization of phosphorus by plants, can improve the plant growth efficiency when being applied to a fertilizer, and can effectively prevent and control the occurrence of plant anthracnose; the attapulgite is modified and calcined, the activity is enhanced, and the dispersing capacity of the raw materials can be improved after the treatment of the ultrasonic dispersing agent; meanwhile, the added rice hull ash has high specific surface area, enhances the matching effect of the raw materials, promotes the performance of the raw material products to be stronger, and further improves the efficacy of the fertilizer, and the invention adopts the bacillus megaterium material in the patent with the application number of 201710989469.6.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1.
The bacillus megaterium for antagonizing colletotrichum gloeosporioides of the embodiment is obtained by activating bacillus megaterium with live bacteria liquid:
the specific activating treatment method comprises the following steps:
step one, preparation of viable bacteria liquid: mixing 10 parts of sucrose, 5 parts of ammonium sulfate and 1 part of iron sulfate heptahydrate, then adding 1 part of ferrous sulfate, and then adjusting the pH value to 7.5 to obtain a viable bacteria solution;
and step two, feeding the bacillus megaterium into live bacteria liquid for culturing, wherein the culture temperature is 26 ℃, and finishing the culture.
The biological fertilizer comprises the following raw materials in parts by weight:
10 parts of bacillus megatherium, 5 parts of rice hull ash, 1 part of potassium dihydrogen phosphate and 1 part of modified attapulgite
And 5 parts of sodium alginate.
The modification method of the modified attapulgite in this example was: calcining the attapulgite for 10min at the calcining temperature of 500 ℃, cooling to 60 ℃ after the calcining is finished, then sending the attapulgite into a dispersant for ultrasonic dispersion for 10min at the ultrasonic power of 500W, then taking out the attapulgite, washing with water and drying to obtain the modified attapulgite.
The preparation method of the dispersant of the embodiment comprises the following steps: the preparation method of the dispersant comprises the following steps: adding polyethylene glycol into an acetone solvent according to the weight ratio of 1:3, then adding sodium citrate accounting for 10% of the total weight of the polyethylene glycol, then adding phosphoric acid, and adjusting the pH value to 6.0 to obtain the dispersing agent.
The rice hull ash of the embodiment is obtained by performing charcoal burning on rice hulls at the charcoal burning temperature of 1000 ℃, and then performing proton irradiation treatment.
The power of the proton irradiation treatment in this example was 500W, and the irradiation time was 25 min.
The charcoal burning temperature in this example was 1000 ℃.
Example 2.
The bacillus megaterium capable of antagonizing colletotrichum gloeosporioides of the embodiment is obtained by activating live bacteria liquid of the bacillus megaterium:
the specific activating treatment method comprises the following steps:
step one, preparation of viable bacteria liquid: mixing 20 parts of sucrose, 10 parts of ammonium sulfate and 5 parts of iron sulfate heptahydrate, then adding 5 parts of ferrous sulfate, and then adjusting the pH value to 7.8 to obtain viable bacteria liquid;
and step two, feeding the bacillus megaterium into live bacteria liquid for culturing, wherein the culture temperature is 30 ℃, and finishing the culture.
The biological fertilizer comprises the following raw materials in parts by weight:
20 parts of bacillus megatherium, 7.5 parts of rice hull ash, 3.5 parts of monopotassium phosphate, 3.5 parts of modified attapulgite and 7.5 parts of sodium alginate.
The modification method of the modified attapulgite in this example was: calcining the attapulgite for 20min at the calcining temperature of 600 ℃, cooling to 60 ℃ after the calcining is finished, then sending the attapulgite into a dispersant for ultrasonic dispersion for 20min with the ultrasonic power of 700W, then taking out the attapulgite, washing with water and drying to obtain the modified attapulgite.
The preparation method of the dispersant of the embodiment comprises the following steps: the preparation method of the dispersant comprises the following steps: adding polyethylene glycol into an acetone solvent according to the weight ratio of 1:3, then adding sodium citrate accounting for 20% of the total weight of the polyethylene glycol, then adding phosphoric acid, and adjusting the pH value to 6.0 to obtain the dispersing agent.
The rice hull ash of the embodiment is obtained by performing charcoal burning on rice hulls at 1500 ℃, and then performing proton irradiation treatment.
The power of the proton irradiation treatment in this example was 700W, and the irradiation time was 35 min.
The charcoal burning temperature in this example was 1500 ℃.
Example 3.
The bacillus megaterium capable of antagonizing colletotrichum gloeosporioides of the embodiment is obtained by activating live bacteria liquid of the bacillus megaterium:
the specific activating treatment method comprises the following steps:
step one, preparation of viable bacteria liquid: mixing 15 parts of sucrose, 7.5 parts of ammonium sulfate and 3 parts of iron sulfate heptahydrate, then adding 3 parts of ferrous sulfate, and then adjusting the pH value to 7.6 to obtain a viable bacteria solution;
and step two, feeding the bacillus megaterium into live bacteria liquid for culturing, wherein the culture temperature is 28 ℃, and finishing the culture.
The biological fertilizer comprises the following raw materials in parts by weight:
20 parts of bacillus megatherium, 7.5 parts of rice hull ash, 3.5 parts of monopotassium phosphate, 3.5 parts of modified attapulgite and 7.5 parts of sodium alginate.
The modification method of the modified attapulgite in this example was: calcining the attapulgite for 15min at the calcining temperature of 550 ℃, cooling to 60 ℃ after the calcining is finished, then sending the attapulgite into a dispersing agent for ultrasonic dispersion for 15min with the ultrasonic power of 600W, then taking out the attapulgite, washing and drying to obtain the modified attapulgite.
The preparation method of the dispersant of the embodiment comprises the following steps: the preparation method of the dispersant comprises the following steps: adding polyethylene glycol into an acetone solvent according to the weight ratio of 1:3, then adding sodium citrate accounting for 10-20% of the total weight of the polyethylene glycol, then adding phosphoric acid, and adjusting the pH value to 6.0 to obtain the dispersing agent.
The rice hull ash of the embodiment is obtained by performing charcoal burning on rice hulls at 1250 ℃, and then performing proton irradiation treatment.
The power of the proton irradiation treatment in this example was 600W, and the irradiation time was 30 min.
The charcoal burning temperature of this example was 1250 ℃.
Comparative example 1.
The only difference from example 3 is that no modified attapulgite was added.
The plants were used for testing, and the performance of the products of examples 1-3 and comparative example 1 was tested as follows:
| group of | Plant growth rate (%) |
| Example 1 | 78 |
| Example 2 | 75 |
| Example 3 | 79 |
| Comparative example 1 | 62 |
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. A bacillus megaterium which can antagonize colletotrichum gloeosporioides is characterized in that,
the bacillus megaterium is obtained by activating bacillus megaterium with viable bacteria liquid:
the specific activating treatment method comprises the following steps:
step one, preparation of viable bacteria liquid: mixing 10-20 parts of sucrose, 5-10 parts of ammonium sulfate and 1-5 parts of iron sulfate heptahydrate, then adding 1-5 parts of ferrous sulfate, and then adjusting the pH value to 7.5-7.8 to obtain viable bacteria liquid;
and step two, feeding the bacillus megaterium into live bacteria liquid for culturing, wherein the culture temperature is 26-30 ℃, and finishing the culture.
2. The biological fertilizer as claimed in claim, characterized by comprising the following raw materials in parts by weight:
10-30 parts of bacillus megatherium, 5-10 parts of rice hull ash, 1-6 parts of monopotassium phosphate, 1-6 parts of modified attapulgite and 5-10 parts of sodium alginate.
3. The biological fertilizer as claimed in claim 2, characterized in that the biological fertilizer comprises the following raw materials in parts by weight:
20-30 parts of bacillus megatherium, 7-10 parts of rice hull ash, 3-5 parts of monopotassium phosphate, 2-5 parts of modified attapulgite and 6-9 parts of sodium alginate.
4. The biological fertilizer according to claim 3, characterized in that the biological fertilizer comprises the following raw materials in parts by weight:
20 parts of bacillus megatherium, 7.5 parts of rice hull ash, 3.5 parts of monopotassium phosphate, 3.5 parts of modified attapulgite and 7.5 parts of sodium alginate.
5. The biofertilizer of claim 4, wherein the modification method of the modified attapulgite is: calcining the attapulgite for 10-20min at the calcining temperature of 500-600 ℃, cooling to 60 ℃ after the calcining is finished, then sending the attapulgite into a dispersant for ultrasonic dispersion for 10-20min at the ultrasonic power of 500-700W, then taking out the attapulgite, washing and drying the attapulgite to obtain the modified attapulgite.
6. The biological fertilizer as claimed in claim 5, wherein the dispersant is prepared by a method comprising: the preparation method of the dispersant comprises the following steps: adding polyethylene glycol into an acetone solvent according to the weight ratio of 1:3, then adding sodium citrate accounting for 10-20% of the total weight of the polyethylene glycol, then adding phosphoric acid, and adjusting the pH value to 6.0 to obtain the dispersing agent.
7. The biofertilizer according to claim 6, wherein the rice husk ash is obtained by charcoal-burning rice husk at 1000-1500 ℃ and then subjecting the rice husk ash to proton irradiation treatment.
8. The biofertilizer of claim 7, wherein the power of proton irradiation treatment is 500-700W, and the irradiation time is 25-35 min.
9. The biofertilizer of claim 8, wherein said proton irradiation treatment power is 600W and irradiation time is 30 min.
10. The biological fertilizer as claimed in claim 7, wherein the charcoal burning temperature is 1000-1500 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210264928.5A CN114574395A (en) | 2022-03-17 | 2022-03-17 | Bacillus megaterium and biofertilizer capable of antagonizing colletotrichum gloeosporioides |
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| CN202210264928.5A CN114574395A (en) | 2022-03-17 | 2022-03-17 | Bacillus megaterium and biofertilizer capable of antagonizing colletotrichum gloeosporioides |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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