CN114569513B - Acne-removing antibacterial skin care product and preparation method thereof - Google Patents
Acne-removing antibacterial skin care product and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an acne-removing antibacterial skin care product and a preparation method thereof. The skin care product comprises the following components in percentage by weight: 25 to 40 percent of active extract, 2 to 5 percent of pine needle extract, 0.04 to 0.48 percent of citric acid modified mixture, 5 to 10 percent of alpha-lipoic acid, 2 to 8 percent of orange leaf essential oil, 0.6 to 1.2 percent of papain, 5 to 10 percent of humectant, 0.2 to 0.8 percent of preservative, 1 to 6 percent of acne-removing antibacterial extract and the balance of water. The invention also provides a preparation method of the composite. Compared with the prior art, the acne-removing antibacterial skin care product has good whitening, yellowing-removing and moisturizing effects, can effectively penetrate through the stratum corneum of the skin to act on the skin, lighten the acne marks and reduce the skin inflammation.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to an acne-removing antibacterial skin care product and a preparation method thereof.
Background
Propionibacterium acnes is an intracellular parasitic bacterium belonging to the normal flora of skin, is gram-positive bacillus cereus, is arranged in X, Y, V and grid shapes, has no capsule, no flagellum, is obligate anaerobic, has small colony, is round, is off-white and is opaque. Acne is a chronic inflammatory skin disease which is frequently generated in adolescent population, clinically manifested as pustules, nodules, acnes, pimples and cysts, and is mostly seen in areas such as face, chest and back, and the like, and the acne is used for treating improper skin damage which is easy to cause pigmentation, scar left and the like. The pathogenesis of acne is complex, mainly related to the factors such as sebum secretion, microbial proliferation, sex hormone level, abnormal keratosis of the pilosebaceous ducts, inflammation and the like.
Propionibacterium acnes has been shown to play an important role in a number of pathogenic events. Propionibacterium acnes can be detected in acne-suffering parts such as non-inflammatory acne, inflammatory papules and pustules, bacteria are detected in skin lesions of non-inflammatory acne and mainly Propionibacterium acnes, and a large number of staphylococcus aureus can be detected in inflammatory skin lesions of acne in addition to Propionibacterium acnes. However, inhibition against propionibacterium acnes has been recently reported. Develops an acne-removing antibacterial skin care product with important functions.
The invention patent with publication number of CN108653159A discloses a scar and acne mark removing skin care product, which comprises the following main raw materials in parts by weight: 5-15 g of brucea javanica, 12-21 g of angelica sinensis, 21-30 g of safflower, 11-19 g of trogopterus dung, 12-24 g of radix stemonae, 18-22 g of peach kernel, 26-36 g of red sage root, 15-30 g of gallnut, 6-21 g of borneol, 9-18 g of phellodendron bark, 12-19 g of white mulberry root-bark, 9-18 g of rhizoma typhonii and 2-3 g of borax. The beneficial effects of the invention are as follows: the product can be made into liquid and paste, is convenient for users to select according to conditions, has no side effect on users, is safe to use, has good effect of repairing new and old scars, has quick acne removing effect, does not leave acne marks, and is not repeated. However, the method has the defects of poor acne removal and whitening effects and poor skin wettability due to the presence of a large number of Chinese herbal medicines.
The invention patent with publication number of CN110897921A discloses an acne-removing skin care product containing a regenerative medical material, wherein the acne-removing skin care product containing the regenerative medical material comprises a regenerative medical material and an active substance, the regenerative medical material is a POSS-based regenerative medical material, and the acne-removing skin care product has a cage-shaped structure formed by Si-O-Si bond frames of silicon oxides, and calcium and phosphorus elements are inlaid in the cage-shaped structure; the active has ion resistance and one or more of sebum hypersecretion reduction, antibacterial, bacteriostatic, anti-inflammatory, anti-allergic or moisturizing properties. The acne-removing skin care product has the advantages of simple components, quick acne-removing effect and easy preservation. However, the acne-removing skin care product containing the regenerated medical material has low transmittance, poor acne-removing effect and no whitening effect.
Disclosure of Invention
In view of the defects of water supplementing, low utilization rate of active substances, poor acne removing and antibacterial effects, slow skin effect of the active substances and the like in the prior art during use, the invention aims to solve the technical problem of obtaining the acne removing and antibacterial skin care product with good water supplementing and acne removing effects, wherein the active substances can effectively act on the inner part of the skin through the surface layer of the skin.
The invention provides an acne-removing antibacterial skin care product, which is characterized by comprising the following components in percentage by weight: 25 to 40 percent of active extract, 2 to 5 percent of pine needle extract, 0.05 to 0.54 percent of citric acid modified mixture, 5 to 10 percent of alpha-lipoic acid, 2 to 8 percent of orange leaf essential oil, 0.06 to 0.12 percent of papain, 5 to 10 percent of humectant, 0.2 to 0.8 percent of preservative, 1 to 6 percent of acne-removing antibacterial extract and the balance of water.
Preferably, the active extract is prepared by the following method, and the parts are all parts by weight:
step 1, respectively washing cowberry leaves and picked pumpkin leaves with water, drying until the cowberry leaves and the picked pumpkin leaves can be kneaded, grinding and sieving to obtain pumpkin leaf powder and cowberry leaf powder, sealing and freezing for preservation;
step 2, mixing 200-500 parts of the cowberry leaf powder prepared in the step 1, 100-300 parts of the pumpkin leaf powder prepared in the step 1 and 1500-4000 parts of the aqueous solution, stirring at 100-110 ℃ and condensing and refluxing for 1-3 hours, stopping condensing and refluxing to obtain a primary extraction mixture, stirring, heating and condensing the primary extraction mixture at 55-75 ℃, collecting volatile liquid, and freeze-drying to obtain active volatile oil; heating and evaporating the rest primary extraction mixture at 100-110 ℃ until the volume becomes 1/4-1/2 of the original volume, adding 600-2000 parts of absolute ethyl alcohol, stirring and heating at 65-85 ℃ and condensing and refluxing for 1-3 hours, filtering, collecting filtrate, heating and evaporating at 80-100 ℃ until solids appear in the filtrate, vacuum drying at 80-100 ℃ for 6-8 hours, mixing with active volatile oil, irradiating with ultraviolet light for 30-60 minutes, sealing and freezing for preservation, and obtaining the active extract.
Preferably, the drying temperature in the step 1 is 40-55 ℃.
Preferably, the grinding condition in the step 1 is 300-500 rpm for 30-60 minutes, and the grinding condition is 80-200 meshes.
Preferably, in the step 1, the sealed freezing preservation is to vacuumize the material and preserve the material at the temperature of-10 to-16 ℃.
Preferably, the stirring speed in the step 2 is 300-500 rpm.
Preferably, the filtration in the step 2 is performed by using one of water-based filter papers having pore diameters of 0.2 μm, 0.22 μm and 0.45 μm.
Preferably, the freeze drying time in the step 2 is 24-48 hours.
Skin irritation is a common skin inflammatory response that manifests as localized skin erythema, itching, burning, stinging and other subjective symptoms. Mainly because the inflammatory factors, sebum and hormone levels secreted by lymphocytes after stimulation by external pathogens are related.
Preferably, the preparation steps of the acne-removing antibacterial extract are as follows:
s1, cleaning azalea leaves and mangosteen pericarps with water, cutting into pieces, drying, grinding into powder, and sieving to prepare functional powder;
s2, adding the functional powder obtained in the step S1 into dichloromethane, performing ultrasonic treatment, evaporating under reduced pressure, filtering and concentrating; drying to obtain acne-removing antibacterial extract.
Further preferably, the preparation steps of the acne-removing antibacterial extract are as follows, and the parts are all parts by weight:
s1, cleaning 10-30 parts of azalea leaves and 40-60 parts of mangosteen pericarp with water, cutting into small blocks, drying at 50-65 ℃ for 40-50 hours, grinding the dried small blocks into fine powder, and screening by a 100-300 mesh sieve to prepare functional powder;
s2, adding the functional powder obtained in the step S1 into 2000-3000 parts of dichloromethane, adopting ultrasonic treatment, evaporating, filtering and concentrating the extracting solution at 35-45 ℃ under reduced pressure, wherein the pressure is 0.05-0.2 MPa; and then drying for 1-3 hours at 35-50 ℃ in a dryer to obtain the acne-removing antibacterial extract.
The 1, 4-benzopyran backbone (chromone) of α -mangosteen and the prenyl side chains and substitution positions thereof may play an important role in their antibacterial activity. Alpha-mangosteen has an inhibitory effect on beta-bamboo lactamase, and in addition, alpha-acyl mangosteen damages peptidoglycan and cytoplasmic membrane, resulting in leakage of intracellular material. But also has synergistic effect with the antibacterial activity of azalea acid. Its synergistic bacteriostatic activity may be related to the destruction of bacterial cell membranes by azaleic acid. The increase of the permeability of bacterial cell membranes makes alpha thin mangosteen enter cells more easily, and plays a stronger antibacterial role by inhibiting the synthesis of bacterial DNA.
Preferably, the ultrasonic extraction temperature in the step S2 is 32-37 ℃, the frequency is 40-50 kHz, and the time is 5-15 min.
Preferably, the citric acid modified mixture is prepared as follows:
d1, adding 0.01 to 0.06 part of citric acid into 100 to 250 parts of absolute ethyl alcohol, adding chitosan after complete dissolution, stirring for 30 to 60 minutes at 400 to 600 revolutions per minute, stirring and heating at 95 to 105 ℃ at 400 to 600 revolutions per minute until the absolute ethyl alcohol is completely evaporated, obtaining a citric acid modified mixture,
preferably, in the step D1, the weight part ratio of citric acid to chitosan is 1:4 to 8.
Preferably, the pine needle extract is the extract of pine needle of pine tree, including the extract of pine needle of Pinus massoniana, pinus koraiensis.
Preferably, the humectant is tea seed oil and hyaluronic acid with a weight ratio of 1.5: (0.1-1.2).
Preferably, the preservative is p-hydroxyacetophenone and limonene in a weight ratio of 1: the mixture of (1-3).
The invention also provides a preparation method of the acne-removing antibacterial skin care product, which comprises the following preparation steps: adding the active extract, the pine needle extract, the citric acid modified mixture, alpha-lipoic acid, orange leaf essential oil, papain, humectant, preservative and acne-removing antibacterial extract into water, stirring for 30-90 minutes, homogenizing for 30-90 minutes at 6000-8000 rpm, and irradiating with ultraviolet light for 30-60 minutes to obtain the acne-removing antibacterial skin care product; the stirring speed is 400-600 rpm.
According to the invention, the water extracts of pumpkin leaves and cowberry leaves are used as main active substances, pine needle extracts and orange leaf essential oil are used as auxiliary materials, and acne-removing antibacterial extracts extracted from azalea leaves and mangosteen peels are added, so that the skin care product has the acne-removing antibacterial moisturizing effect, can effectively repair skin dryness, quicken skin scar healing, and maintain skin saline-alkali balance. The papain is compounded with the chitosan grafted with the citric acid, so that the force of the active ingredients of the skin product on the inner layer of the skin through skin cutin is increased, the effect of the skin care product is effectively improved, and the growth of harmful bacteria on the surface of the skin is inhibited when the skin care product is acted on the skin. The p-hydroxyacetophenone and the limonene are mixed to serve as preservatives, antibacterial and antiseptic effects of the skin care product during storage are enhanced, meanwhile, the limonene also has the effects of degreasing and antioxidation, skin grease balance is balanced, alpha, mangosteen and azalea acid enter skin hair follicles, mass propagation of parasitic pathogenic microorganisms can be restrained, and occurrence of skin inflammation is effectively relieved.
Due to the adoption of the scheme, compared with the prior art, the invention has the following functions: 1) The skin care product has the functions of acne removal, bacteriostasis and moisture preservation, can improve the penetration of skin care product active substances into deep layers of skin through stratum corneum, and improves the action effect of the skin care product; 2) Can effectively relieve skin inflammation and repair skin dryness, and accelerate skin scar healing; 3) Can inhibit mass proliferation of pathogenic microorganisms, lighten vaccinia, and reduce skin inflammation.
The folium Vaccinii Vitis-idaeae plant Vaccinium myrtillus L.leaf is also called bearberry leaf, and is obtained by collecting leaf during 6 months of flowering, sun drying, and drying. The folium Vaccinii Vitis-idaeae contains arbutin, ursolic acid, pear glycoside, mao Liudai, 4-hydroxyphenyl, beta-gentiobioside, hyperin, avicularin, quercetin 3-D-glucose-L-rhamnoside. 2-o-caffeoyl arbutin and isoquercitrin. And 7.9 to 8.2 percent of burnt catechol type tannins, has the effects of detoxification, diuresis and the like.
Pine needle extract is needle leaf extracted from pine tree, and the pine is the long of all kinds of wood, and the leaves, skin and ointment are mainly used for treating rheumatism, wind pain, hair growth, five viscera safety, yang strengthening, middle-jiao strengthening, hunger eliminating, life prolonging, tooth strengthening, face retaining, skin jade and body weight reducing. There are many records of health care and disease treatment effects of pine needles in ancient books of pharmacopoeias in China, and pine needles with life-promoting functions are listed as 'medicine feeding' in "Ben Cao gang mu" in Li Shizhen. Recent scientific research shows that pine needles contain abundant flavonoid substances, vitamins (vitamin C, vitamin A, beta-carotene and vitamin E, B), various amino acids and mineral elements, and can prevent and treat influenza, night blindness, rheumatism and strengthen body. At present, the medicinal use of pine needles and pine trees is more widespread in the united states, france, spanish, etc. The pine needle extract is rich in natural water-soluble bioflavonoid concentrate, and is a compound antioxidant substance. The pine needle extract can obviously reduce the cancerogenic hazard of three traditional foods of fumigation, curing and fermentation, and is a natural anti-gene mutation food. Deep processing to obtain brown tablet, which can be directly administered with water.
The chitosan is prepared from chitin as raw material by deacetylation, and has chemical name of polyglucosamine (1-4) -2-amino-B-D glucose, and is insoluble in water, soluble in dilute acid, and absorbable by human body. Chitosan is a primary derivative of chitin. The chemical structure of the polymer is a high molecular basic polysaccharide polymer with cations, and the polymer has unique physical and chemical properties and biological activation functions.
Lipoic acid is coenzyme existing in mitochondria, enters cells after being absorbed by intestinal tracts in vivo, has the characteristics of fat solubility and water solubility, and can pass through the whole body without resistance to reach any cell part, thereby providing comprehensive efficacy for human body. Alpha-lipoic acid is also called lipoic acid or caprylic acid, is a powerful antioxidant, and can help cells to convert glucose into energy, and has the effects of detoxifying the body, resisting skin inflammation, stabilizing blood sugar and the like. The water-soluble and fat-soluble organic compound can permeate the tissues such as nervous system and heart mainly composed of fat and water, thus protecting them from free radical damage and cleaning them out of the body before the free radicals damage cells. Lipoic acid also helps the body to more effectively utilize vitamins E and C, as well as other antioxidants such as glutathione. This nutrient also allows vitamin B to be more effective in converting protein, carbohydrate and fat into energy.
Papain, also called papain for short, is a pure natural biological enzyme product extracted by using milk in immature papaya fruits and adopting modern biological engineering technology, is a low-specificity proteolytic enzyme, and widely exists in roots, stems, leaves and fruits of papaya, wherein the content of the papaya enzyme in the immature milk is most abundant. It has strong hydrolytic capacity to animal and plant protein, polypeptide, ester, amide, etc. and also has synthetic function, and can synthesize protein hydrolysate into protein. Papain is a sulfhydryl (-SH) endopeptidase, has the activities of protease and esterase, contains cysteine in the active center, belongs to the sulfhydryl protease, has the characteristics of high enzyme activity, good heat stability, natural sanitation, safety and the like, is dissolved in water and glycerol, and is colorless or light yellow in water solution and sometimes milky; is hardly dissolved in organic solvents such as ethanol, chloroform, diethyl ether and the like. Most suitable for PH value of 6-7 (generally 3-9.5), and has effect in neutral or slightly acidic, isoelectric point of 8.75; the most suitable temperature is 55-65 ℃ (generally 10-85 ℃ all the time), the heat resistance is strong, and the heat cannot be completely inactivated at 90 ℃; is inhibited by an oxidizing agent and the reducing substance is activated. Papain consists of 212 amino acid residues, and after 2/3 peptide chains in molecules are hydrolyzed from N terminal by aminopeptidase, the remaining 1/3 peptide chains still keep 99% of activity, so that the papain has wide application in industries such as food, medicine, feed, daily chemicals, leather, textile and the like.
Hyaluronic acid, also known as hyaluronic acid, is an acidic mucopolysaccharide. It is an important basic substance for skin care, is a component of human body, has special moisturizing effect, has the weight up to 100 times of the weight of the skin care, is the substance with the best moisturizing property in the nature, and is called ideal natural moisturizing factor. It can improve skin nutrition metabolism, make skin tender, smooth, remove wrinkle, increase elasticity, prevent aging, and is good transdermal absorption promoter while keeping moisture.
Detailed Description
The specific raw material sources of the embodiment are as follows:
pumpkin leaves were purchased from green organic vegetable gardens of vegetable farmers.
Cowberry leaf: in the producing area, salty yang is present.
Azalea leaf: and (3) at the production place, yellow oka.
Mangosteen pericarp: malaysia at the place of production.
Chitosan: molecular weight of sienga and biotechnology limited: 1526.4539 degree of deacetylation: greater than 90%.
Pine needle extract: xian Guangdong Biotechnology Limited liability company, goods number: TGY-1650, content: more than or equal to 99 percent.
Orange leaf essential oil: shenzhen city Dingcheng plant perfume limited company, product number: DC-054, pale yellow liquid.
Papain: henan Zhengxing food additive Co., ltd., preservation rate of enzyme activity: 99%, enzyme activity: 10 ten thousand, goods number: 1001, arsenic content is 0.00003%.
Tea seed oil: jian city Zhongxiang natural plant Co., ltd., model: jc-0037, pale yellow to yellow liquid, relative density: 0.912 to 0.927.
Limonene: wuhan belcat biomedical limited, CAS number: 7705-14-8.
Example 1
A preparation method of an anti-acne antibacterial skin care product comprises the following preparation steps:
30g of active extract, 3.5g of pine needle extract, 0.3g of citric acid modified mixture, 7g of alpha-lipoic acid, 5g of orange leaf essential oil, 0.1g of papain, 4.2g of tea seed oil, 2.8g of hyaluronic acid, 0.2g of p-hydroxyacetophenone and 0.4g of limonene are added into 46.5g of water, stirred for 60 minutes at 500 rpm, homogenized for 60 minutes at 7000 rpm, and irradiated by ultraviolet light for 40 minutes, so that the acne-removing antibacterial skin care product is obtained.
The active extract is prepared by the following method:
step 1, respectively washing 300g of cowberry leaves and 200g of picked pumpkin leaves with water, drying at 45 ℃ until the cowberry leaves and the cowberry leaves can be kneaded, ball-milling at 400 revolutions per minute for 45 minutes, sieving with a 100-mesh sieve to obtain pumpkin leaf powder and cowberry leaf powder, vacuumizing and then preserving at the temperature of minus 14 ℃;
step 2, mixing 300g of the cowberry leaf powder prepared in the step 1, 150g of the pumpkin leaf powder prepared in the step 1 and 2000g of the water solution, stirring at 105 ℃ at 400 rpm, condensing and refluxing for 2 hours, stopping condensing and refluxing to obtain a primary extraction mixture, stirring, heating and condensing the primary extraction mixture at 65 ℃, collecting volatile liquid, and freeze-drying for 36 hours to obtain active volatile oil; evaporating the rest primary extract mixture at 105deg.C until the volume becomes 1/3 of the original volume, adding 1000g of anhydrous ethanol, stirring at 75deg.C, heating under reflux for 2 hr, filtering with 0.22 μm pore diameter water-based filter paper, collecting filtrate, evaporating at 100deg.C until solid appears in the filtrate, vacuum drying at 85deg.C for 7 hr, mixing with active volatile oil, irradiating with ultraviolet light for 45 min, vacuumizing, and preserving at-14deg.C to obtain active extract.
The citric acid modified mixture is prepared by the following method:
d1, adding 0.04g of citric acid into 150g of absolute ethyl alcohol, adding 0.24g of chitosan after complete dissolution, stirring for 45 minutes at 500 rpm, and heating at 100 ℃ at 500 rpm until the absolute ethyl alcohol is completely evaporated, thus obtaining a citric acid modified mixture.
Example 2
A preparation method of an anti-acne antibacterial skin care product comprises the following preparation steps:
30g of active extract, 3.5g of pine needle extract, 0.3g of citric acid modified mixture, 7g of alpha-lipoic acid, 5g of orange leaf essential oil, 0.1g of papain, 4.2g of tea seed oil, 2.8g of hyaluronic acid, 0.2g of p-hydroxyacetophenone, 0.4g of limonene and 4g of acne-removing antibacterial extract are added into 42.5g of water, stirred for 60 minutes at 500 rpm, homogenized for 60 minutes at 7000 rpm, and irradiated by ultraviolet light for 40 minutes to obtain the acne-removing antibacterial skin care product.
The active extract and citric acid modified mixture were prepared in the same manner as in example 1.
The preparation method of the acne-removing antibacterial extract comprises the following steps:
s1, cleaning 20g of azalea leaves and 50g of mangosteen pericarps with water, cutting into small pieces, drying the small pieces for 45 hours in a hot air oven at 60 ℃, grinding the dried small pieces into fine powder by a grinder, and screening the fine powder by a 200-mesh screen to prepare functional powder;
s2, adding the functional powder obtained in the step S1 into 2500g of dichloromethane, and performing ultrasonic treatment, wherein the ultrasonic extraction temperature is 35 ℃, the frequency is 45kHz, the time is 10min, and the extracting solution is filtered and concentrated under the reduced pressure of a rotary vacuum evaporator at 40 ℃ and the pressure is 0.1MPa; and then drying for 2 hours at 40 ℃ in a dryer to obtain the acne-removing antibacterial extract.
Comparative example 1
A preparation method of an anti-acne antibacterial skin care product comprises the following preparation steps:
30g of active extract, 3.5g of pine needle extract, 0.3g of citric acid modified mixture, 7g of alpha-lipoic acid, 5g of orange leaf essential oil, 0.1g of papain, 4.2g of tea seed oil, 2.8g of hyaluronic acid and 0.6g of p-hydroxyacetophenone are added into 46.5g of water, stirred for 60 minutes at 500 rpm, homogenized for 60 minutes at 7000 rpm, and irradiated by ultraviolet light for 40 minutes, so that the acne-removing antibacterial skin care product is obtained.
The active extract and citric acid modified mixture were prepared in the same manner as in example 1.
Comparative example 2
A preparation method of an anti-acne antibacterial skin care product comprises the following preparation steps:
30g of active extract, 3.5g of pine needle extract, 0.4g of citric acid modified mixture, 7g of alpha-lipoic acid, 5g of orange leaf essential oil, 4.2g of tea seed oil, 2.8g of hyaluronic acid, 0.2g of p-hydroxyacetophenone and 0.4g of limonene are added into 46.5g of water, stirred for 60 minutes at 500 rpm, homogenized for 60 minutes at 7000 rpm, and irradiated by ultraviolet light for 40 minutes, so that the acne-removing antibacterial skin care product is obtained.
The active extract and citric acid modified mixture were prepared in the same manner as in example 1.
Comparative example 3
A preparation method of an anti-acne antibacterial skin care product comprises the following preparation steps:
30g of active extract, 3.5g of pine needle extract, 7g of alpha-lipoic acid, 5g of orange leaf essential oil, 0.4g of papain, 4.2g of tea seed oil, 2.8g of hyaluronic acid, 0.2g of p-hydroxyacetophenone and 0.4g of limonene are added into 46.5g of water, stirred for 60 minutes at 500 rpm, homogenized for 60 minutes at 7000 rpm, and irradiated by ultraviolet light for 40 minutes to obtain the acne-removing antibacterial skin care product.
The active extract was prepared in the same manner as in example 1.
Comparative example 4
A preparation method of an anti-acne antibacterial skin care product comprises the following preparation steps:
30g of active extract, 3.5g of pine needle extract, 7g of alpha-lipoic acid, 5g of orange leaf essential oil, 4.2g of tea seed oil, 2.8g of hyaluronic acid, 0.2g of p-hydroxyacetophenone and 0.4g of limonene are added into 46.5g of water, stirred for 60 minutes at 500 rpm, homogenized for 60 minutes at 7000 rpm, and irradiated by ultraviolet light for 40 minutes to obtain the acne-removing antibacterial skin care product.
The active extract was prepared in the same manner as in example 1.
Comparative example 5
A preparation method of an anti-acne antibacterial skin care product comprises the following preparation steps:
3.5g of pine needle extract, 0.3g of citric acid modified mixture, 7g of alpha-lipoic acid, 5g of orange leaf essential oil, 0.1g of papain, 4.2g of tea seed oil, 2.8g of hyaluronic acid, 0.2g of p-hydroxyacetophenone and 0.4g of limonene are added into 46.5g of water, stirred for 60 minutes at 500 rpm, homogenized for 60 minutes at 7000 rpm, and irradiated by ultraviolet light for 40 minutes to obtain the acne-removing antibacterial skin care product.
The preparation method of the citric acid modified mixture is the same as in example 1.
Test example 1
Whitening, yellow-removing and moisturizing test:
the whitening, yellowing and moisturizing test of the skin care product is examined according to the following method: the skin care products prepared in examples 1-2 and comparative examples 1-5 of the present invention were used after washing the face in the morning and evening, respectively, were used for selecting 42 volunteers, the skin care products were prepared in the same diet after washing the face in the morning and evening, the brightness of the skin before and after the volunteers were tested was measured by using a color difference meter in the test period of 3 months, the average value of each group was taken, the skin tenderness and smoothness improvement condition was evaluated by observing the touch, the evaluation was classified into three grades with obvious improvement, general effect and no change, and the test results are shown in table 1.
Table 1 whitening, anti-yellowing and moisturizing test results
( Remarks: + indicates an increase, -indicates a decrease, and the more the change in skin brightness indicates a better whitening effect )
As can be seen by comparing examples 1-2 with comparative examples 1-4, the skin whitening effect of example 1 is better, and the skin tendering and smoothness improvement effect of comparative examples 3-5 is general because the effect of promoting the absorption and utilization of the whitening and moisturizing substances by the skin is better after the papain and the citric acid modified mixture are compounded. The citric acid modified mixture has the effects of moisture absorption and moisture preservation, acts on the inside of skin, enables the skin to be more moist and glossy, and can promote the skin care product to be absorbed by the skin, thereby effectively improving the rough skin condition and enabling the skin to be tender and smooth. Comparative example 5 comparative example 1 shows that the whitening effect is very different, and it is possible that the active extract contains various active substances to improve the brightness of skin, so as to achieve the effects of whitening and eliminating yellow.
Test example 2
Active permeation test:
the active substance of the invention is examined by taking alpha-lipoic acid as a test target substance through a test and referring to journal papers (preparation of arbutin chitosan nanoparticles and in vitro transdermal absorption research, author: wang Zanli, northwest pharmaceutical journal, 2021, month 1), and the specific steps are as follows: SD rats were sacrificed by removing abdominal hair and cutting the abdomen 3.14cm 2 Removing skin adipose tissue, washing with normal saline, removing excessive water, and cutting mouse skinThe stratum corneum faces the supply tank, the dermis faces the receiving tank, the stratum corneum is fixed in the middle of the Franz diffusion tank, the volume of the receiving solution is 8mL of physiological saline, the temperature of the water bath is kept at (32+/-0.5), skin care product samples (each containing 7mg of alpha-lipoic acid) are respectively taken and added into the supply tank for transdermal experiments, 0.5mL of receiving solution is taken from the receiving tank within 3 hours, the supernatant is centrifugally taken, the supernatant is diluted to proper mass concentration, and the alpha-lipoic acid concentration C is detected and calculated by liquid chromatography i The cumulative transdermal amount is calculated as in formula 1.
V 0 -receiving volume (8 mL);
a- -skin transdermal area (3.14 cm) 2 )
TABLE 2 alpha lipoic acid 3 hours cumulative transdermal amount
By comparing examples 1-2 with comparative examples 1-5, it was found that the cumulative permeation of alpha-lipoic acid of example 1 was superior to comparative examples 1-5, which is caused by the papain and citric acid modified mixture opening the skin stratum corneum channel, promoting the permeation of alpha-lipoic acid through the stratum corneum. When the papain and the citric acid modified mixture are compounded, the effect of promoting alpha-lipoic acid to penetrate through the stratum corneum is better than that of the papain and the citric acid modified mixture. Example 1 was compared with comparative example 1 and found that the cumulative transdermal amount of alpha-lipoic acid of example 1 was higher than that of comparative example 1, probably because the addition of limonene increased the rate of alpha-lipoic acid penetration through the stratum corneum.
Test example 3
Acne removal and bacteriostasis test:
test of test object bacteriostasis rate: propionibacterium acnes strain (ATCC 11827) and Staphylococcus aureus strain (CICC B0142) were taken and diluted to 1.0X108 CFU/mL with sterilized physiological saline, respectively. Propionibacterium acnes strain and staphylococcus aureus strain are respectively inoculated on a surface dish culture medium by a 6 x 6 streaking method on an ultra-clean workbench, and each strain of each sample adopts one culture medium. The culture medium was perforated with a sterilized punch, 5 for each medium. The samples of examples 1 and 2 were filled into a 0.1mL volume using a disposable sterile syringe. After pipetting, placing a propionibacterium acnes strain culture dish in an anaerobic culture tank at 35 ℃ for 48 hours, placing a staphylococcus aureus strain culture dish in an aerobic culture tank at 37 ℃ for 48 hours, and then measuring the diameter of a bacteriostasis ring of each sample liquid to be measured by using a vernier caliper, wherein the average value of the test results is shown in table 3.
TABLE 3 acne removal and bacteriostasis test results
From the test results, it can be seen that the diameter of the inhibition zone is larger in example 2, to which the acne-removing antibacterial extract is added, than in example 1, to which no acne-removing antibacterial extract is added, probably because the alpha-mangosteen in the extracted mangosteen pericarp has an inhibitory effect on beta-lactamase, and furthermore, the alpha-mangosteen can damage peptidoglycan and cytoplasmic membrane, resulting in leakage of intracellular substances. Moreover, the azalea acid extracted from the azalea leaves can destroy bacterial cell membranes, and the increase of the permeability of the bacterial cell membranes enables the alpha-mangosteen to enter cells more easily, so that a synergistic effect is generated, and a stronger antibacterial effect is exerted by inhibiting the synthesis of bacterial DNA.
Claims (5)
1. An acne-removing antibacterial skin care product is characterized by comprising the following components: 25-40% of active extract, 2-5% of pine needle extract, 0.05-0.54% of citric acid modified mixture, 5-10% of alpha-lipoic acid, 2-8% of orange leaf essential oil, 0.06-0.12% of papain, 5-10% of humectant, 0.2-0.8% of preservative, 1-6% of acne-removing antibacterial extract and the balance of water;
the preparation method of the active extract comprises the following steps:
step 1, cleaning pumpkin leaves and cowberry leaves with water, drying until the pumpkin leaves and cowberry leaves can be kneaded, grinding and sieving to obtain pumpkin leaf powder and cowberry leaf powder, sealing and freezing for preservation;
step 2, mixing the cowberry leaf powder prepared in the step 1, the pumpkin leaf powder prepared in the step 1 and water, heating, stirring, condensing and refluxing to obtain a primary extraction mixture, stirring, heating, condensing and collecting volatile liquid at 55-75 ℃, and freeze-drying to obtain active volatile oil; heating and evaporating the rest primary extraction mixture until the volume is 1/4-1/2 of the original volume, adding absolute ethyl alcohol, stirring, heating, condensing and refluxing, filtering, collecting filtrate, heating and evaporating until solid appears in the filtrate, vacuum drying, mixing with active volatile oil, irradiating with ultraviolet light, sealing, freezing and preserving to obtain active extract;
the citric acid modified mixture is obtained by mixing citric acid, absolute ethyl alcohol and chitosan, stirring for 30-60 minutes, stirring and heating at 95-105 ℃ until the absolute ethyl alcohol is completely evaporated; the weight ratio of the citric acid to the chitosan is 1 (4-8);
the preparation method of the acne-removing antibacterial extract comprises the following steps:
s1, cleaning azalea leaves and mangosteen pericarps with water, cutting into pieces, drying, grinding into powder, and sieving to prepare functional powder;
s2, adding the functional powder obtained in the step S1 into dichloromethane, performing ultrasonic treatment, evaporating under reduced pressure, filtering and concentrating; drying to obtain acne-removing antibacterial extract.
2. The acne-removing antibacterial skin care product according to claim 1, wherein the ultrasonic treatment temperature in the step S2 is 32-37 ℃, the frequency is 40-50 kHz, and the time is 5-15 min.
3. The acne-removing antibacterial skin care product as claimed in claim 1, wherein: the humectant is prepared from tea seed oil and hyaluronic acid in a weight ratio of 1.5: (0.1-1.2).
4. The acne-removing antibacterial skin care product as claimed in claim 1, wherein: the preservative is prepared from p-hydroxyacetophenone and limonene in a weight ratio of 1: (1-3).
5. The method for preparing the acne-removing antibacterial skin care product according to any one of claims 1-4, which is characterized by comprising the following steps: adding the active extract, the pine needle extract, the citric acid modified mixture, alpha-lipoic acid, orange leaf essential oil, papain, a humectant, a preservative and an anti-acne antibacterial extract into water, stirring for 30-90 minutes, homogenizing for 30-90 minutes, and irradiating with ultraviolet light for 30-60 minutes to obtain the anti-acne antibacterial skin care product.
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