CN114540166A - Bioreactor's stabilizing mean is used in biological cell manufacturing - Google Patents
Bioreactor's stabilizing mean is used in biological cell manufacturing Download PDFInfo
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- CN114540166A CN114540166A CN202210052561.0A CN202210052561A CN114540166A CN 114540166 A CN114540166 A CN 114540166A CN 202210052561 A CN202210052561 A CN 202210052561A CN 114540166 A CN114540166 A CN 114540166A
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 23
- 230000000087 stabilizing effect Effects 0.000 title claims abstract description 17
- 238000005070 sampling Methods 0.000 claims abstract description 51
- 230000007246 mechanism Effects 0.000 claims abstract description 16
- 238000004659 sterilization and disinfection Methods 0.000 claims description 11
- 230000006978 adaptation Effects 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 230000000149 penetrating effect Effects 0.000 claims description 9
- 230000006641 stabilisation Effects 0.000 claims description 9
- 238000011105 stabilization Methods 0.000 claims description 9
- 238000007789 sealing Methods 0.000 claims description 7
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims 2
- 235000017491 Bambusa tulda Nutrition 0.000 claims 2
- 241001330002 Bambuseae Species 0.000 claims 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims 2
- 239000011425 bamboo Substances 0.000 claims 2
- 238000000034 method Methods 0.000 abstract description 9
- 230000008569 process Effects 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 4
- 238000002955 isolation Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 17
- 238000001914 filtration Methods 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008827 biological function Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 206010011409 Cross infection Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 206010028347 Muscle twitching Diseases 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
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- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/14—Disinfection, sterilisation or deodorisation of air using sprayed or atomised substances including air-liquid contact processes
- A61L9/145—Disinfection, sterilisation or deodorisation of air using sprayed or atomised substances including air-liquid contact processes air-liquid contact processes, e.g. scrubbing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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Abstract
The invention discloses a stabilizing mechanism of a bioreactor for manufacturing biological cells, and relates to the field of bioreactors. This biological cell makes with bioreactor's stabilizing mean is including cultivateing cauldron, sampler and aseptic case, cultivates the side fixedly connected with sampler of cauldron, the aseptic case of side fixedly connected with of sampler, the lower fixed surface of cultivateing the cauldron is connected with the landing leg, and the top of cultivateing the cauldron is fixed the joint and is had sealed lid. This biological cell makes with bioreactor's stabilizing mean can realize the isolation operation of sample process through sampler and the aseptic case of activity, prevent that the sample from being contaminated, also avoid taking a sample many times to cultivate the environment production influence in cultivateing the cauldron, the joint mode through to the sampling bottle makes things convenient for placing and taking of sampling bottle, and this device is lower to the requirement of sampling bottle, accord with the sample needs in the in-service use, can directly add through the intake pipe when needs add a small amount of materials, avoid opening sealed lid and cause the cultivation cauldron to pollute.
Description
Technical Field
The invention relates to the technical field of biological cell manufacturing, in particular to a stabilizing mechanism of a bioreactor for biological cell manufacturing.
Background
The bioreactor is a device system for carrying out biochemical reaction in vitro by utilizing the biological function of enzyme or organism (such as microorganism), is a biological function simulator, such as a fermentation tank, an immobilized enzyme or an immobilized cell reactor, and has important application in the aspects of wine, medicine production, jam concentration, fruit juice fermentation and organic pollutant degradation.
When the bioreactor is used for serum-free full suspension culture of cells, the growth condition of the cells can be detected according to the sampling inspection operation of the culture in the reactor, usually, a sampling bottle is connected with a tank body through a valve and a hose, a sample is pumped into the sampling bottle from a reactor, then the sample is pumped out to an external sample receiving device by the sampling bottle, but because the sampling bottle is positioned outside the reactor, the environment maintaining system in the reactor can not be maintained, so that the residual sample after sampling in the sampling bottle is remained in the sampling bottle, the next sampling or inoculation process is polluted, the existing bioreactor is not convenient for the disassembly, assembly and cleaning of the sampling bottle, and the sealing treatment of the communication part of the sampling bottle and the reactor is not perfect, which is not beneficial to the use of the bioreactor, therefore, one skilled in the art proposes a stabilization mechanism for a bioreactor for manufacturing biological cells to solve the above-mentioned problems.
Disclosure of Invention
Technical problem to be solved
In order to overcome the defects in the prior art, the invention discloses a stabilizing mechanism of a bioreactor for manufacturing biological cells, which aims to solve the problems in the background technology.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides a biological cell makes stabilizing mean of bioreactor for, includes cultivates cauldron, sampler and aseptic box, the side fixedly connected with sampler of cultivateing the cauldron, the side fixedly connected with aseptic box of sampler, the sampler includes mounting panel, push-out drum, first diaphragm, second diaphragm and sampling bottle, mounting panel fixed connection is in the side of cultivateing the cauldron, the side fixedly connected with push-out drum of mounting panel, the side of mounting panel just is located the first diaphragm of fixedly connected with above the push-out drum, the side of mounting panel just is located push-out drum below sliding connection and has the second diaphragm, the last fixed surface joint of second diaphragm has the sampling bottle, aseptic box includes airtight ware, filter tube, intake pipe, check valve, curb plate, draw-in groove, fixture block and tight position spring, airtight ware has been seted up to the upper surface of first diaphragm, the inboard fixed joint of airtight ware has the filter tube, the lateral wall fixedly connected with intake pipe of filter cylinder, the one end and the feed cylinder fixed connection of filter cylinder are kept away from to the intake pipe, the inner wall fixedly connected with check valve of intake pipe, the last fixed surface of second diaphragm is connected with the curb plate, the draw-in groove has been seted up to the upper surface of second diaphragm, the inner wall sliding clamping of draw-in groove has the fixture block, the tight position spring of side fixedly connected with of fixture block.
Preferably, the lower surface of the culture kettle is fixedly connected with a supporting leg, and the top end of the culture kettle is fixedly clamped with a sealing cover.
Preferably, the side of mounting panel runs through and sets up the through-hole with push cylinder looks adaptation, the push cylinder runs through the through-hole and extends to the cultivation cauldron inboard, just the lateral wall and the through-hole inner wall fixed connection of push cylinder.
Preferably, the side fixedly connected with slide rail of mounting panel, one side that the second diaphragm is close to the mounting panel is provided with the spout with slide rail looks adaptation, the second diaphragm passes through spout and mounting panel sliding connection, the fixed joint of sample bottle is inboard at the draw-in groove.
Preferably, the fixed snap ring that is provided with of interior bottom surface of airtight ware, the inner wall of filter cylinder is provided with the spacing ring with snap ring looks adaptation, just the intussuseption of airtight ware is filled with disinfectant.
Preferably, the top fixedly connected with L-shaped piece of curb plate, the lower surface of first diaphragm is provided with the recess, and the inner wall of recess is pegged graft and is had the stopper with L-shaped piece looks adaptation, the side fixedly connected with reset spring of stopper.
Preferably, the two opposite lateral walls of the second transverse plate symmetrically penetrate through and are provided with clamping bolts matched with the slide rails, the side faces of the clamping bolts are fixedly connected with abutting springs, one ends, far away from the clamping bolts, of the abutting springs are fixedly connected with abutting plates, and one sides, far away from the abutting springs, of the clamping bolts are provided with limiting teeth.
Preferably, one end of the push cylinder penetrating through the culture kettle is fixedly connected with a one-way valve, and the inner wall of the push cylinder is connected with a piston in a sliding manner.
Preferably, cultivate the lower fixed surface of cauldron and be connected with the drain pipe, the drain pipe is kept away from and is cultivateed cauldron one end and run through the upper surface of first diaphragm and extend to the sampling bottle top, the drain pipe is close to the one end fixedly connected with check valve of sampling bottle.
The invention discloses a stabilizing mechanism of a bioreactor for manufacturing biological cells, which has the following beneficial effects:
this biological cell makes stabilizing mean of bioreactor for manufacturing, inject the air after filtering inflator filtration disinfection into the culture kettle through the pusher that runs through the setting to cultivate the cauldron side, the culture solution in will cultivating the cauldron is from the drain pipe extrusion of fixed connection at the culture kettle lower surface, and go into in the sample bottle of fixed joint at second diaphragm upper surface, on the one hand through the filterable air admission of airtight ware culture kettle helps sample postculture cauldron internal gas pressure balance, and the air after the disinfection can not pollute and cultivate the interior environment of culture kettle, on the other hand the cross infection of the interior external environment of culture kettle is stopped to the check valve that sets up respectively, the stability of the interior environment of culture kettle is cultivateed in the protection.
This biological cell makes and uses bioreactor's stabilizing mean, the interior bottom surface of airtight ware passes through the snap ring and strains the fixed joint of air cylinder, and the interpolation is used for the sterile disinfection liquid of filtration in airtight ware, when twitching the piston in the pusher, because the effect of check valve, the pusher can only inhale gas from the intake pipe, make and strain the interior atmospheric pressure of air cylinder and descend, strain the air cylinder and inhale the air from airtight ware outward, the air is through disinfection liquid's filtration, can prevent effectively that microorganism from the air in the intake pipe from getting into to cultivate the cauldron in to the culture environment production influence, guarantee to cultivate the cauldron internal environment and be difficult to because sample analysis or segmentation sampling cause the destruction of culture cauldron internal environment.
This biological cell makes with bioreactor's stabilizing mean, the aseptic box includes the disinfection part of the inboard air of cauldron and the protection two-sided to the sample environment to getting into cultivation, the stopper with first diaphragm lower surface of second diaphragm fixed connection's curb plate top fixed connection's L-shaped piece cooperation, and combine second diaphragm sliding connection's connected mode, form the enclosed environment through with curb plate and first diaphragm joint at sample inoculation in-process, cultivate the sample work of cultivateing the cell in the cauldron in the enclosed environment, pull the stopper and remove the second diaphragm to keeping away from first diaphragm department and carry out the change cleaning work of sampling bottle after accomplishing the sample work, can less cultivation cauldron internal environment because of the contaminated probability of sample.
This stabilizing mean of bioreactor for biological cell manufacturing, the screens is tied and is had and support tight board through supporting tight spring coupling, and the effect through supporting tight spring and supporting tight board is tied the screens tightly on spacing tooth to reach and tie spacing effect to the screens, make the second diaphragm can stop on the slide rail arbitrary high department, conveniently access and clear up the sampling bottle of joint at second diaphragm upper surface.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is an overall cross-sectional view of the present invention;
FIG. 3 is an enlarged view of the structure of FIG. 2;
FIG. 4 is an enlarged view of the structure of FIG. 3 at B in accordance with the present invention;
FIG. 5 is an enlarged view of the mounting plate structure of the present invention;
FIG. 6 is a schematic view of a first cross plate structure according to the present invention;
FIG. 7 is a schematic view of a limiting structure of the present invention;
fig. 8 is a schematic view of the structure of the locking bolt of the present invention.
In the figure: 1. a culture kettle; 2. a sampler; 201. mounting a plate; 202. a push cylinder; 203. a first transverse plate; 204. a second transverse plate; 205. sampling a bottle; 3. a sterile box; 301. an airtight vessel; 302. a gas filter cylinder; 303. an air inlet pipe; 304. a one-way valve; 305. a side plate; 306. a card slot; 307. a clamping block; 308. a tightening spring; 4. a support leg; 5. a sealing cover; 6. a through hole; 7. a slide rail; 8. a chute; 9. a snap ring; 10. a limiting ring; 11. an L-shaped block; 12. a limiting block; 13. a return spring; 14. a clamping bolt; 15. a propping plate; 16. limiting teeth; 17. a piston; 18. a liquid outlet pipe.
Detailed Description
The embodiment of the invention discloses a stabilizing mechanism of a bioreactor for manufacturing biological cells, which comprises a culture kettle 1, a sampler 2 and an aseptic tank 3, wherein the sampler 2 is fixedly connected to the side surface of the culture kettle 1, the aseptic tank 3 is fixedly connected to the side surface of the sampler 2, a supporting leg 4 is fixedly connected to the lower surface of the culture kettle 1, and a sealing cover 5 is fixedly clamped at the top end of the culture kettle 1, as shown in figures 1-8.
The sampler 2 comprises a mounting plate 201, a push cylinder 202, a first transverse plate 203, a second transverse plate 204 and a sampling bottle 205, the mounting plate 201 is fixedly connected to the side surface of the culture kettle 1, the side surface of the mounting plate 201 is fixedly connected with the push cylinder 202, the side surface of the mounting plate 201 is fixedly connected with the first transverse plate 203 above the push cylinder 202, the side surface of the mounting plate 201 is slidably connected with the second transverse plate 204 below the push cylinder 202, and the sampling bottle 205 is fixedly clamped on the upper surface of the second transverse plate 204.
The aseptic box 3 comprises an airtight container 301, an air filter cylinder 302, an air inlet pipe 303, a one-way valve 304, a side plate 305, a clamping groove 306, a clamping block 307 and a clamping spring 308, the airtight container 301 is arranged on the upper surface of a first transverse plate 203, the air filter cylinder 302 is fixedly clamped on the inner side of the airtight container 301, the air inlet pipe 303 is fixedly connected to the side wall of the air filter cylinder 302, one end, far away from the air filter cylinder 302, of the air inlet pipe 303 is fixedly connected with a push cylinder 202, the inner wall of the air inlet pipe 303 is fixedly connected with the one-way valve 304, the upper surface of a second transverse plate 204 is fixedly connected with the side plate 305, the clamping groove 306 is arranged on the upper surface of the second transverse plate 204, the clamping block 307 is clamped on the inner wall of the clamping groove 306 in a sliding mode, and the clamping spring 308 is fixedly connected to the side surface of the clamping block 307.
The side of the mounting plate 201 is provided with a through hole 6 matched with the push cylinder 202 in a penetrating manner, the push cylinder 202 penetrates through the through hole 6 and extends to the inner side of the culture kettle 1, and the side wall of the push cylinder 202 is fixedly connected with the inner wall of the through hole 6.
The side fixedly connected with slide rail 7 of mounting panel 201, one side that second diaphragm 204 is close to mounting panel 201 is provided with the spout 8 with slide rail 7 looks adaptation, and second diaphragm 204 passes through spout 8 and mounting panel 201 sliding connection, and the fixed joint of sampling bottle 205 is inboard at draw-in groove 306.
The inner bottom surface of the airtight dish 301 is fixedly provided with a clamping ring 9, the inner wall of the air filtering cylinder 302 is provided with a limiting ring 10 matched with the clamping ring 9, and the airtight dish 301 is filled with disinfection liquid.
The top fixedly connected with L-shaped piece 11 of curb plate 305, the lower surface of first diaphragm 203 is provided with the recess, and the inner wall of recess is pegged graft and is had stopper 12 with the 11 looks adaptations of L-shaped piece, and the side fixedly connected with reset spring 13 of stopper 12.
The two opposite side walls of the second transverse plate 204 are symmetrically provided with clamping bolts 14 matched with the slide rails 7 in a penetrating manner, the side surfaces of the clamping bolts 14 are fixedly connected with abutting springs, one ends of the abutting springs, far away from the clamping bolts 14, are fixedly connected with abutting plates 15, and one sides of the clamping bolts 14, far away from the abutting springs, are provided with limiting teeth 16.
One end of the push cylinder 202 penetrating the culture kettle 1 is fixedly connected with a one-way valve 304, and the inner wall of the push cylinder 202 is connected with a piston 17 in a sliding way.
The lower surface fixedly connected with drain pipe 18 of cultivateing cauldron 1, drain pipe 18 keep away from cultivateing cauldron 1 one end and run through the upper surface of first diaphragm 203 and extend to sampling bottle 205 top, and drain pipe 18 is close to the one end fixedly connected with check valve 304 of sampling bottle 205.
Example 1: referring to fig. 1-5, a stabilizing mechanism of a bioreactor for manufacturing biological cells includes a culture kettle 1, a sampler 2 and an aseptic tank 3, the sampler 2 is fixedly connected to a side surface of the culture kettle 1, the aseptic tank 3 is fixedly connected to a side surface of the sampler 2, the sampler 2 includes a mounting plate 201, a push cylinder 202, a first transverse plate 203, a second transverse plate 204 and a sampling bottle 205, the mounting plate 201 is fixedly connected to a side surface of the culture kettle 1, the push cylinder 202 is fixedly connected to a side surface of the mounting plate 201, the first transverse plate 203 is fixedly connected to a side surface of the mounting plate 201 and above the push cylinder 202, the second transverse plate 204 is slidably connected to a side surface of the mounting plate 201 and below the push cylinder 202, and the sampling bottle 205 is fixedly clamped to an upper surface of the second transverse plate 204.
The lower surface of the culture kettle 1 is fixedly connected with a supporting leg 4, and the top end of the culture kettle 1 is fixedly clamped with a sealing cover 5.
The side of the mounting plate 201 is provided with a through hole 6 matched with the push cylinder 202 in a penetrating manner, the push cylinder 202 penetrates through the through hole 6 and extends to the inner side of the culture kettle 1, and the side wall of the push cylinder 202 is fixedly connected with the inner wall of the through hole 6.
The side fixedly connected with slide rail 7 of mounting panel 201, one side that second diaphragm 204 is close to mounting panel 201 is provided with the spout 8 with slide rail 7 looks adaptation, and second diaphragm 204 passes through spout 8 and mounting panel 201 sliding connection, and the fixed joint of sampling bottle 205 is inboard at draw-in groove 306.
The two opposite side walls of the second transverse plate 204 are symmetrically provided with clamping bolts 14 matched with the slide rails 7 in a penetrating manner, the side surfaces of the clamping bolts 14 are fixedly connected with abutting springs, one ends of the abutting springs, far away from the clamping bolts 14, are fixedly connected with abutting plates 15, and one sides of the clamping bolts 14, far away from the abutting springs, are provided with limiting teeth 16.
One end of the push cylinder 202 penetrating the culture kettle 1 is fixedly connected with a one-way valve 304, and the inner wall of the push cylinder 202 is connected with a piston 17 in a sliding way.
The lower surface fixedly connected with drain pipe 18 of cultivateing cauldron 1, drain pipe 18 keep away from cultivateing cauldron 1 one end and run through the upper surface of first diaphragm 203 and extend to sampling bottle 205 top, and drain pipe 18 is close to the one end fixedly connected with check valve 304 of sampling bottle 205.
In this embodiment, the sampler 2 is disposed in the sterile box 3, by pushing and pulling the push cylinder 202 in the sampler 2, the piston 17 moves to compress air, and the air is injected into the culture kettle 1 through the one-way valve 304, the air pressure inside the culture kettle 1 increases, the culture solution is pressed out from the liquid outlet pipe 18 and enters the sampling bottle 205 fixedly clamped on the upper surface of the second horizontal plate 204, after the sampling bottle 205 completes the sampling process, the clamping bolt 14 is separated from the limit of the limit teeth 16 by pressing the fastening plate 15, the second horizontal plate 204 is moved away from the first horizontal plate 203, then the sampling bottle 205 fixedly clamped on the upper surface of the second horizontal plate 204 is taken out, the sampling bottle 205 is clamped on the upper surface of the second horizontal plate 204 through the clamping groove 306 and the clamping block 307, thereby facilitating the replacement and cleaning of the sampling bottle 205, and the second horizontal plate 204 forms a sterile operation space in the sampling process through the sliding connection manner of the sliding rail 7 and the sliding groove 8, reduce the pollution of the external air to the sampling environment in the sampling process, thereby avoiding the influence on the internal environment of the culture kettle 1 due to the sampling.
Example 2: referring to fig. 1-8, a stabilizing mechanism of a bioreactor for manufacturing biological cells comprises a culture kettle 1, a sampler 2 and an aseptic tank 3, wherein the sampler 2 is fixedly connected to the side surface of the culture kettle 1, the aseptic tank 3 is fixedly connected to the side surface of the sampler 2, the aseptic tank 3 comprises an airtight dish 301, an air filter 302, an air inlet pipe 303, a one-way valve 304, a side plate 305, a clamping groove 306, a clamping block 307 and a clamping spring 308, the airtight dish 301 is arranged on the upper surface of a first transverse plate 203, the air filter 302 is fixedly clamped on the inner side of the airtight dish 301, the air inlet pipe 303 is fixedly connected to the side wall of the air filter 302, one end of the air inlet pipe 303, which is far away from the air filter 302, is fixedly connected to a pushing cylinder 202, the one-way valve 304 is fixedly connected to the inner wall of the air inlet pipe 303, the side plate 305 is fixedly connected to the upper surface of a second transverse plate 204, the clamping groove 306 is arranged on the upper surface of the second transverse plate 204, the clamping groove 306 is slidably clamped with the clamping block 307, a clamping spring 308 is fixedly connected to the side surface of the clamping block 307.
The lower surface of the culture kettle 1 is fixedly connected with a supporting leg 4, and the top end of the culture kettle 1 is fixedly clamped with a sealing cover 5.
The inner bottom surface of the airtight dish 301 is fixedly provided with a clamping ring 9, the inner wall of the air filtering cylinder 302 is provided with a limiting ring 10 matched with the clamping ring 9, and the airtight dish 301 is filled with disinfection liquid.
In this embodiment, a snap ring 9 is fixedly connected to an inner bottom surface of the airtight vessel 301, the snap ring 9 is concentrically arranged with the airtight vessel 301, the air filter cylinder 302 is fixedly clamped with the snap ring 9 through a stop ring 10 fixedly connected to a bottom end, the sterilization liquid is added into the airtight vessel 301, and the sterilization liquid does not cover the bottom end of the air filter cylinder 302, when the push cylinder 202 sucks air from the air filter cylinder 302 through the air inlet pipe 303, a negative pressure is formed inside the air filter cylinder 302, the negative pressure causes the air filter cylinder 302 to suck air from the outside, the air is sterilized by the sterilization liquid in the process of entering the air filter cylinder 302, so as to prevent the culture environment in the culture kettle 1 from being polluted by the heterogeneous fungi when the outside air directly enters the culture kettle 1, and the first horizontal plate 203 and the second horizontal plate 204 are fixedly clamped through the side plate 305 in the aseptic tank 3, a closed sampling environment is formed in the sampling process, and the sample probability in the sampling process is effectively reduced, the sampling bottle 205 is fixedly clamped in the clamping groove 306 through the clamping block 307, so that the sampling bottle 205 with different specifications can be used in the device, and the sampling bottle 205 is convenient to take and fix, thereby avoiding the problem that the sampling bottle 205 is inconvenient to clean.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (9)
1. The utility model provides a biological cell makes stabilizing mean with bioreactor, includes culture kettle (1), sampler (2) and aseptic box (3), its characterized in that: cultivate side fixedly connected with sampler (2) of cauldron (1), side fixedly connected with aseptic box (3) of sampler (2), sampler (2) are including mounting panel (201), push cylinder (202), first diaphragm (203), second diaphragm (204) and sampling bottle (205), mounting panel (201) fixed connection is in the side of cultivating cauldron (1), the side fixedly connected with push cylinder (202) of mounting panel (201), the side of mounting panel (201) just is located push cylinder (202) top fixedly connected with first diaphragm (203), the side of mounting panel (201) just is located push cylinder (202) below sliding connection has second diaphragm (204), the last fixed surface joint of second diaphragm (204) has sampling bottle (205), aseptic box (3) are including airtight ware (301), a gas filter section of thick bamboo (302), intake pipe (303), Check valve (304), curb plate (305), draw-in groove (306), fixture block (307) and tight position spring (308), airtight ware (301) have been seted up to the upper surface of first diaphragm (203), the inboard fixed joint of airtight ware (301) has gas filter tube (302), the lateral wall fixedly connected with intake pipe (303) of gas filter tube (302), the one end and the push away a section of thick bamboo (202) fixed connection of gas filter tube (302) are kept away from in intake pipe (303), the inner wall fixedly connected with check valve (304) of intake pipe (303), the last fixed surface of second diaphragm (204) is connected with curb plate (305), draw-in groove (306) have been seted up to the upper surface of second diaphragm (204), the inner wall slip joint of draw-in groove (306) has fixture block (307), the side fixedly connected with tight position spring (308) of fixture block (307).
2. The stabilization mechanism of a bioreactor for manufacturing biological cells according to claim 1, wherein: the lower surface of the culture kettle (1) is fixedly connected with supporting legs (4), and the top end of the culture kettle (1) is fixedly clamped with a sealing cover (5).
3. The stabilization mechanism of a bioreactor for manufacturing biological cells according to claim 1, wherein: the side of mounting panel (201) runs through-hole (6) of seting up with push cylinder (202) looks adaptation, push cylinder (202) run through-hole (6) and extend to cultivation cauldron (1) inboard, just the lateral wall and through-hole (6) inner wall fixed connection of push cylinder (202).
4. The stabilization mechanism of a bioreactor for manufacturing biological cells according to claim 1, wherein: the side fixedly connected with slide rail (7) of mounting panel (201), one side that second diaphragm (204) is close to mounting panel (201) is provided with spout (8) with slide rail (7) looks adaptation, second diaphragm (204) are through spout (8) and mounting panel (201) sliding connection, it is inboard at draw-in groove (306) to take sample bottle (205) fixed joint.
5. The stabilization mechanism of a bioreactor for manufacturing biological cells according to claim 1, wherein: the inner bottom surface of the airtight dish (301) is fixedly provided with a clamping ring (9), the inner wall of the filter gas cylinder (302) is provided with a limiting ring (10) matched with the clamping ring (9), and the airtight dish (301) is filled with disinfection liquid.
6. The stabilization mechanism of a bioreactor for manufacturing biological cells according to claim 1, wherein: the top fixedly connected with L shape piece (11) of curb plate (305), the lower surface of first diaphragm (203) is provided with the recess, and the inner wall of recess is pegged graft and is had stopper (12) with L shape piece (11) looks adaptation, the side fixedly connected with reset spring (13) of stopper (12).
7. The stabilization mechanism of a bioreactor for manufacturing biological cells according to claim 1, wherein: two lateral walls opposite to the second transverse plate (204) are symmetrically provided with clamping bolts (14) matched with the sliding rails (7) in a penetrating mode, the side faces of the clamping bolts (14) are fixedly connected with abutting springs, one ends, far away from the clamping bolts (14), of the abutting springs are fixedly connected with abutting plates (15), and one sides, far away from the abutting springs, of the clamping bolts (14) are provided with limiting teeth (16).
8. The stabilization mechanism of a bioreactor for manufacturing biological cells according to claim 1, wherein: one end of the push cylinder (202) penetrating through the culture kettle (1) is fixedly connected with a one-way valve (304), and the inner wall of the push cylinder (202) is connected with a piston (17) in a sliding manner.
9. The stabilization mechanism of a bioreactor for manufacturing biological cells according to claim 1, wherein: cultivate lower surface fixedly connected with drain pipe (18) of cauldron (1), drain pipe (18) are kept away from and are cultivateed cauldron (1) one end and run through the upper surface of first diaphragm (203) and extend to sample bottle (205) top, drain pipe (18) are close to one end fixedly connected with check valve (304) of sample bottle (205).
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| CN202210052561.0A CN114540166A (en) | 2022-01-17 | 2022-01-17 | Bioreactor's stabilizing mean is used in biological cell manufacturing |
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| CN202210052561.0A CN114540166A (en) | 2022-01-17 | 2022-01-17 | Bioreactor's stabilizing mean is used in biological cell manufacturing |
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| JP2000097952A (en) * | 1998-09-24 | 2000-04-07 | Marubishi Baioenji:Kk | Preparatively isolating apparatus for sample solution |
| CN205115498U (en) * | 2015-11-10 | 2016-03-30 | 信达生物制药(苏州)有限公司 | Bioreactor's sampling device |
| CN209231027U (en) * | 2018-11-29 | 2019-08-09 | 河套学院 | A kind of sampler of bioreactor |
| US20210024869A1 (en) * | 2019-07-25 | 2021-01-28 | Nihon Kohden Corporation | Sterile sampling apparatus |
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2022
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000097952A (en) * | 1998-09-24 | 2000-04-07 | Marubishi Baioenji:Kk | Preparatively isolating apparatus for sample solution |
| CN205115498U (en) * | 2015-11-10 | 2016-03-30 | 信达生物制药(苏州)有限公司 | Bioreactor's sampling device |
| CN209231027U (en) * | 2018-11-29 | 2019-08-09 | 河套学院 | A kind of sampler of bioreactor |
| US20210024869A1 (en) * | 2019-07-25 | 2021-01-28 | Nihon Kohden Corporation | Sterile sampling apparatus |
| CN213239597U (en) * | 2020-10-22 | 2021-05-18 | 无锡格瑞普恩医疗科技有限公司 | Sampling device is collected to infectious disease source |
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