JP2000097952A - Preparatively isolating apparatus for sample solution - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a preparatively isolating apparatus by which a sample solution can be sampled precisely into a metering container by correcting the sampling level of the sampling solution automatically to a metering level. SOLUTION: The upper-part side inside a sealed metering container 2 and the bottom-part side inside a culture solution tank 3 in which a sample solution is stored are made to communicate with each other by a first transport pipe 4. A second transport pipe 6 which transports the sample solution inside the metering container 2 to a test tube 8 is attached to the bottom part of the metering container 2. A first pinch valve 11 and a second pinch valve 12 for an opening and closing operation are installed at the first transport pipe 4 and the second transport pipe 6. A sensor 24 which detects the level of the sample solution inside the metering container 2 and which outputs a signal at the level of a metering operation is installed at the metering container 2. In addition, a suction pump 18 which discharges an atmosphere inside the container 2 from the upper part of the container 2 to the metering container 2 and a pressurization pump 19 which pressurizes the level of the sample solution inside the metering container 2 are connected via a filter 14.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sample liquid collecting apparatus for collecting a sample liquid from a microorganism culturing apparatus or an enzyme reaction apparatus, and more particularly to a sample liquid collecting apparatus capable of automatically collecting a sample liquid. is there.

[0002]

2. Description of the Related Art In general, a series of operations such as an operation of collecting a sample from a microorganism culturing device or an oxygen reaction device at predetermined time intervals and a cleaning operation after collection are performed by a technician. Operating by operating pumps and valves.

[0003] Since a sample liquid needs to be collected at predetermined time intervals in a sample liquid collecting operation, a conventional collecting method performed manually requires a technician to work at night or on holidays. It is hard work. In addition, since it depends on human labor, there is a possibility that a problem may occur in the collection time, the collection amount, the collection quality, and the like.

[0004]

Therefore, a sample liquid separating apparatus shown in FIG. 3 has been proposed. As shown in the figure, the sample liquid separating device 30 communicates a bottom side in a sealed measuring vessel 31 with a bottom side in a culture solution tank 32 by a transport pipe 33, and has an upper part in the measuring vessel 31. Is connected to a supply / discharge pump 36 via a supply / discharge pipe 35 having a filter 34, a second transport pipe 37 is branched from the transport pipe 33, and a first pinch valve 38 is provided in the middle of the second transport pipe 37. A second pinch valve 39 is provided on the upstream side of the branch of the transport pipe 33 (on the side of the culture solution tank), and the first pinch valve 38 is opened and the second pinch valve 39 is closed. A fixed amount of the sample liquid is collected from the culture solution tank 32 by the pressure drop in the measuring vessel 31, and the second transport pipe 37 is placed in a state where the pipe end faces the inlet of the collection vessel such as the test tube 8. 1
This is a device in which the pinch valve 38 is closed, the second pinch valve 39 is opened, and the sample liquid is transferred to the test tube 8 by pressurizing the liquid level of the measuring container 31 by the supply / discharge pump 36. At this time, due to the closing timing of the second pinch valve 38, the viscosity difference of the culture solution, and the inertial rotation of the supply pump 36, the liquid level of the measuring container 31 tends to increase more than the fixed amount. There is a drawback that it is difficult to set the amount of the sample liquid.

Therefore, there arises a technical problem to be solved in order to automatically correct the collected liquid level of the sample liquid to a fixed liquid level and to collect an accurate sample liquid in the measuring container. Aims to solve the problem.

[0006]

SUMMARY OF THE INVENTION The present invention has been proposed to solve the above-mentioned object, and has an upper side in a sealed measuring container and a lower side in a sample liquid tank for storing a sample liquid. And a second transport pipe for transporting the sample liquid from the measuring vessel to the sample liquid collecting vessel is provided at the bottom of the measuring vessel, and the first transport pipe and the second transport pipe are connected to the first transport pipe and the second transport pipe. A first pinch valve and a second pinch valve for opening and closing are respectively provided, and a sensor for detecting a liquid level of a sample liquid in the measuring container and outputting a signal when the liquid level is constant is provided in the measuring container. A sample liquid collecting apparatus, wherein a suction pump for discharging the atmosphere in the container from the upper portion of the measuring container and a pressurizing pump for pressurizing the liquid surface of the sample liquid in the measuring container are connected to the measuring container via a filter. And an outlet in the measuring container of the first transport pipe When the sample start signal is input by a timer, the first pinch valve is opened and the second pinch valve is closed, and a signal is output from the sensor when the sample start signal is input by a timer. Activate the suction pump until the sample liquid is collected in the measuring container until the signal is output from the sensor, stop the suction pump and close the first pinch valve,
After opening the second pinch valve, to provide a sample liquid sorting apparatus provided with a controller that operates the pressure pump to transfer the sample liquid from the measuring container to the sample liquid collection container,
In addition, one end of the first transport pipe extending from the inside of the measuring container to the exposed portion outside the measuring container is formed of a metal tube,
A thin metal rod is extended upward from the bottom side in the closed container to penetrate the ceiling of the measuring container, and one of the metal rod and the metal tube is used as an anode or a cathode, and the other is a cathode or an anode. The present invention is to provide a sample liquid collecting apparatus which constitutes the sensor by being electrically connected to corresponding contacts.

[0007]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS One embodiment of the present invention applied to a culture liquid sorting apparatus will be described below in detail with reference to FIGS. FIG. 1 shows a culture liquid sorter as a sample liquid sorter. As shown in FIG. 1, the culture liquid sorter 1 includes a culture liquid tank (a sample liquid tank) in which an elongated cylindrical closed glass container is used as a measurement container 2 and an upper side of the measurement container 2 for storing the culture liquid. ) 3 communicates with the bottom side through a first transport pipe 4 made of silicon tube, and an elongated discharge pipe section 5 is provided at the bottom of the measuring container 2.
And a second transport pipe 6 made of a silicon tube is connected to the discharge pipe section 5, and the discharge port 7 at the tip of the second transport pipe 6 is
It faces the inlet 9 of the test tube 8 as a sample liquid collecting container. Then, the culture solution stored in the culture solution tank 3 made of glass is sucked into the measuring container 2 by suction negative pressure,
After the measurement, pressurized air is supplied into the measuring container 2 and the liquid level is pressurized at the predetermined pressure to transport the culture solution from the inside of the measuring container 2 to the test tube 8.
, A first pinch valve 11 is attached to the second transport pipe 6, and a second pinch valve 12 is attached to the second transport pipe 6.
The transport pipe 4 and the second transport pipe 6 are configured to be openable and closable, respectively. Here, the first pinch valve 11 and the second pinch valve 12 are connected to the first transport pipe 4 and the second transport pipe 6.
Is a valve which is closed radially from the outside, closed by crushing, and opened by releasing the pressing. Therefore, sterilization inside and outside of the first transport pipe 4 and the second transport pipe 6 can be easily performed.
A tubular pipe connection 13 for discharging the atmosphere in the measuring container 2 and supplying pressurized air of a predetermined pressure into the measuring container 2 is integrally formed at an upper portion of the pipe connection.
And a suction and discharge pipe 1 having a filter 14 for cleaning and sterilization.
5 is attached, and the end of the suction / discharge pipe 15 is branched into two branches, and the respective branch pipes 16 and 17 are respectively provided with suction pumps 18.
And the pressurizing pump 19.

Accordingly, the second transport pipe 6 is closed by closing the second pinch valve 12, and the first transport pipe 4 is opened by opening the first pinch valve 11, after which only the suction pump 18 is operated. When the atmosphere in the measuring container 2 is exhausted to the outside, the culture solution in the culture solution tank 3 can be sucked into the measuring container 2 in accordance with the pressure drop in the measuring container 2, and conversely, The two-pinch valve 12 is opened to open the second transport pipe 6, and the first pinch valve 11 is closed to close the first transport pipe 4.
Is operated, the culture solution in the measuring container 2 can be transferred to the test tube 8 via the second transport tube 6 by the pressure of the pressurized air acting on the liquid surface.

The height position of the outlet 20 in the measuring container 2 of the first transport pipe 4 in the measuring container 2 is equal to the level of the fixed amount of the culture solution collected in the measuring container 2 and the fixed amount of the culture solution. It is set so as to be in contact with the liquid level of the liquid. Therefore, when the pressurizing pump 19 is operated while the first pinch valve 11 is opened and the second pinch valve 12 is closed, the surplus of the culture solution is pushed back to the culture solution tank 3 and the fixed liquid level is When the outlet 20 of the first oil feed pipe 4 meets the air in the measuring container 2 at the time, the supply of the culture solution to the first transport pipe 4 is cut off, and the measured amount of the culture solution is Will be collected. As described above, in the present embodiment, the height position of the outlet 20 in the measuring container 2 of the first transport pipe 4 in the measuring container 2 is equal to the level of the fixed amount of culture solution collected in the measuring container 2 and By setting so that it comes into contact with the liquid surface of the fixed amount of the culture solution, excessive suction of the culture solution caused by the closing timing of the pinch valves 11 and 12, the viscosity difference of the culture solution, and the inertia of the pump 16 can be prevented. The air in the measuring container 2 and the outlet 20 of the first transport pipe 4 are used together.
By taking advantage of the impossibility of transmitting pressure due to the occurrence of the above, a fixed amount of culture solution can be obtained.

However, in order to control the opening / closing timing of the pinch valves 11 and 12 and the operation timing of the pumps 18 and 19, a certain standard is required. A sensor for detection is required.

Here, as the sensor, a float type sensor that opens and closes contacts using buoyancy may be used. However, due to the limitation of the capacity of the measuring container 2, cleanliness and sterilization are not required. Considering the difficulty, hiring is difficult.

Therefore, in this embodiment, one end of the first transport pipe 4 extending from the inside of the measuring container outlet 20 to the outside of the measuring container is constituted by the metal tube 21 and the thin metal rod 22 is connected to the metal tube 21. Extending upward from the bottom side in the measuring container 2, penetrating the ceiling of the measuring container 2, one of the metal rod 22 and the metal tube 21 as an anode or a cathode, the other as a cathode or an anode, Each is electrically connected to a corresponding contact (not shown), and a signal when the contact is closed is input to the controller 23.

Therefore, the sensor 24 composed of the metal tube 21 and the metal rod 22 is superior in cleanliness and sterility as compared with the float type sensor, and has a very small influence on the volume of the measuring container 2. Simple and cheap. Further, if the metal tube 21 is used as it is as a part of the sensor 24, it is possible to sense all liquid levels above the fixed liquid level, and it is possible to expand the detection accuracy and the detection range.

The controller 23 comprises a well-known sequencer or microcomputer. The controller 23 automatically collects the culture solution in the measuring container 2 and then opens and closes the pinch valves 11 and 12 to transfer the culture solution to the test tube 8. The operation and stop of each of the pumps 18 and 19 are controlled.

FIG. 2 is a block diagram showing the basic control of the controller 23. As shown in the figure, in this control, first, when there is an ON input of a switch for starting a sample, the controller 23 starts a timer for managing year, day, and time. Then, the apparatus enters the standby mode and waits until the time (year, day, time) set by the timer (step 0).

When a set time is reached by a timer, the first
The pinch valve 11 is opened, the second pinch valve 12 is closed, and then the pressure pump 19 is turned on for a predetermined time (about 10 seconds).
ec) Activate (step 1).

All the deposits in the first transport pipe 4 are blown back to the culture solution tank 3 by the pressure of the pressurized air, and all the high-viscosity culture solution due to the growth of microorganisms in the pipe is discharged out of the tube. Is done. Thereby, the inside of the first transport pipe 4 is cleaned, and the culture solution tank 3 is agitated so that the viscosity thereof becomes uniform.
Of course, depending on the setting of the cleaning time, the microorganisms can be evenly distributed by this stirring.

Next, in order to collect a culture solution, the first pinch valve 11 is opened and the second pinch valve 12 is kept closed, and the suction pump 18 is held until an energization signal is input from the sensor 24. Is started (step 2).

The culture solution is sucked into the measurement container 2 from the culture solution tank 3 by the pressure drop of the measurement container 2, and both the metal tube 21 and the metal rod 22 come into contact with the culture solution and are electrically connected to each other. Then, the controller 23 considers that the collection of the culture solution into the measuring container 2 is completed, stops the suction pump 18, and simultaneously opens the first pinch valve 11 and holds the second pinch valve 12 closed. The pressurizing pump 19 is started for a predetermined time (about 10 seconds).

As a result, a pressing force acts on the surface of the culture solution collected in the measuring container 2, and the surplus of the culture solution is pushed back to the culture solution tank 3 by this pressure (step 3).

After a lapse of a predetermined time (about 10 sec), a drive signal is output to a rotary drive device (step motor or the like) 26 of a support 25 supporting the test tube 8 to test the discharge port 7 of the second transport tube 6. After facing the inlet 9 of the tube 8, a drive signal is output to an actuator (not shown) to open a slide shutter (not shown), thereby opening the inlet 9 of the test tube 8.

After the opening, the pressure pump 19
, The first pinch valve 11 is kept closed, the second pinch valve 12 is opened, and the culture solution is transferred to the test tube 8 by the pressure of the pressurizing pump 19 acting on the liquid level (step 4). ).

The operation of the pressurizing pump 19 and the first
After opening the pinch valve 11 and closing the second pinch valve 12 for a predetermined time (about 5 seconds), the remaining liquid in the first transport pipe 4 is blown back to the culture tank 3, thereby cleaning the inside of the first transport pipe 4. (Step 5).

Further, while the first pinch valve 11 is closed and the second pinch valve 12 is opened for a predetermined time (about 10 seconds), the pressure pump 19 is operated to dry the second transport pipe 6. (Step 6) Subsequently, a drive signal is output to the actuator to close the entrance 9 of the test tube to prevent evaporation of the collected culture solution.

After that, a driving signal is output to the rotary driving device (step motor or the like), and the support table 25 is output.
Is rotated by a predetermined angle, that is, by the pitch of the test tube 8, the next test tube 8 is made to face the discharge port 7 of the second transport tube 12 (Step 7), and the process returns to Step 0. Note that the rotation angle of the support 25 is controlled by the reflection plate 4 as shown in FIG.
0 and the photoelectric switch 41 are used.

As described above, the culture fluid sorter according to the present embodiment simply prevents the influence of the fluid inertia caused by the closing timing of the pinch valves 11 and 12 and the variation in the viscosity of the culture fluid as the sample fluid. Not only to eliminate, but also
Since the first transport pipe 4 is cleaned and the second transport pipe 8 is cleaned and dried to prepare for the next collection of the culture solution, the reliability of automatic collection of the culture solution of a constant quality is improved as much as possible. be able to.

In the present embodiment, a suction pump 18 and a pressure pump 19 are provided,
However, it is also possible to configure the supply and discharge pump 36 and one filter 14,
The present invention includes such a configuration. When it is necessary to control the temperature of the culture liquid sorter 1 depending on the type of the microorganism or the properties of the sample liquid, the culture liquid sorter 1 is naturally installed in a temperature-controlled storage such as a refrigerator. Things.

Further, the present invention can be variously modified without departing from the spirit of the present invention, and it goes without saying that the present invention extends to the modified ones.

[0029]

According to the first aspect of the present invention, as described in detail in the first embodiment, the upper side of the sealed measuring container and the lower side of the sample liquid tank for storing the sample liquid are separated. A second transport pipe is provided at the bottom of the measuring vessel for transporting a sample liquid from the measuring vessel to a sample liquid collecting vessel, and the first transport pipe and the second transport pipe are respectively opened and closed. A first pinch valve and a second pinch valve for
Providing a sensor for detecting the liquid level of the sample liquid in the measuring container in the measuring container and outputting a signal when the liquid level of the fixed amount,
A sample liquid sorting apparatus, wherein a suction pump for discharging the atmosphere in the container from the upper part of the measurement container to the measurement container and a pressurizing pump for pressurizing the liquid surface of the sample liquid in the measurement container are connected via a filter. Setting the outlet inside the measuring container of the first transport pipe to a position in contact with the liquid surface of a fixed amount of sample liquid, and when a sample start signal is input by a timer, opening the first pinch valve; With the valve closed, the suction pump is operated until a signal is output from the sensor to collect a sample liquid in the measuring container, and when a signal is output from the sensor, the suction pump is stopped. After closing the first pinch valve and opening the second pinch valve, a controller was provided to transfer the sample liquid from the measuring container to the sample liquid collection container by operating the pressure pump. Serial Each effect of fluid inertia due to variations in the viscosity of the pinch closing timing and sample liquid valve is eliminated, the sample solution quantitatively is automatically collected.

According to a second aspect of the present invention, one end of the first transport pipe extending from the inside of the measuring container to the exposed portion outside the measuring container is formed of a metal tube, and a thin metal rod is connected to the closed container. Extending upward from the bottom side of the inside and penetrating the ceiling of the measuring container, further, one of the metal rod and the metal tube as an anode or a cathode, the other as a cathode or an anode,
By electrically connecting to each corresponding contact,
Since the sensor is configured and the metal pipe portion is used as it is as a part of the sensor and all the liquid levels above the fixed liquid level are sensed, the detection accuracy can be greatly improved. Further, it is excellent in cleanliness and sterility, has a very small influence on the volume of the measuring container, and has a simple structure and low cost.

[Brief description of the drawings]

FIG. 1 is a configuration diagram of a culture liquid sorter according to an embodiment of a sample liquid separator of the present invention.

FIG. 2 is a block diagram illustrating control contents of a controller.

FIG. 3 is a configuration diagram of a conventional sample liquid sorting apparatus studied.

[Explanation of symbols]

 REFERENCE SIGNS LIST 1 culture liquid collecting device (sample liquid collecting device) 2 measuring container 3 culture liquid tank 4 first transport tube 5 second transport tube 8 test tube (sample liquid collecting container) 11 first pinch valve 12 second pinch valve 14 Filter 18 Suction pump 19 Pressure pump 20 Measuring container outlet 21 Metal tube 22 Metal rod 23 Controller 24 Sensor

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[Procedure amendment]

[Submission date] September 30, 1998 (1998.9.3
0)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] Claim 2

[Correction method] Change

[Correction contents]

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0004

[Correction method] Change

[Correction contents]

[0004]

Therefore, a sample liquid sorting apparatus shown in FIG. 3 has been proposed. As shown in the figure, the sample liquid sorting apparatus 30 communicates the bottom side in the sealed measuring vessel 31 and the bottom side in the culture solution tank 32 with the transport pipe 33, and also the inside of the measuring vessel 31. The upper part is connected to a supply / discharge pump 36 via a supply / discharge pipe 35 having a filter 34, a second transport pipe 37 is branched from the transport pipe 33, and a first pinch valve 38 is provided in the middle of the second transport pipe 37. A second pinch valve 39 is provided on the upstream side of the branch of the transport pipe 33 (on the side of the culture solution tank), the first pinch valve 38 is opened, and the second pinch valve 39 is closed. A constant amount of the sample liquid is collected from the culture solution tank 32 by the pressure drop in the measuring container 31 due to the above, and with the pipe end of the second transport pipe 37 facing the inlet of the collection container such as the test tube 8, First
This is a device in which the pinch valve 38 is closed, the second pinch valve 39 is opened, and the sample liquid is transferred to the test tube 8 by pressurizing the liquid level of the measuring container 31 by the supply / discharge pump 36. At this time, due to the closing timing of the second pinch valve 38, the viscosity difference of the culture solution, and the inertial rotation of the supply pump 36, the liquid level of the measuring container 31 tends to increase more than the fixed amount. There is a drawback that it is difficult to set the amount of the sample liquid.

[Procedure amendment 3]

[Document name to be amended] Statement

[Correction target item name] 0006

[Correction method] Change

[Correction contents]

[0006]

SUMMARY OF THE INVENTION The present invention has been proposed to solve the above-mentioned object, and has an upper side in a sealed measuring container and a lower side in a sample liquid tank for storing a sample liquid. And a second transport pipe for transporting the sample liquid from the measuring vessel to the sample liquid collecting vessel is provided at the bottom of the measuring vessel, and the first transport pipe and the second transport pipe are connected to the first transport pipe and the second transport pipe. A first pinch valve and a second pinch valve for opening and closing are respectively provided, and a sensor for detecting a liquid level of a sample liquid in the measuring container and outputting a signal when the liquid level is constant is provided in the measuring container. A sample liquid collecting apparatus, wherein a suction pump for discharging the atmosphere in the container from the upper portion of the measuring container and a pressurizing pump for pressurizing the liquid surface of the sample liquid in the measuring container are connected to the measuring container via a filter. And an outlet in the measuring container of the first transport pipe When the sample start signal is input by a timer, the first pinch valve is opened and the second pinch valve is closed, and a signal is output from the sensor when the sample start signal is input by a timer. Activate the suction pump until the sample liquid is collected in the measuring container until the signal is output from the sensor, stop the suction pump and close the first pinch valve,
After opening the second pinch valve, to provide a sample liquid sorting apparatus provided with a controller that operates the pressure pump to transfer the sample liquid from the measuring container to the sample liquid collection container,
In addition, one end of the first transport pipe extending from the inside of the measuring container to the exposed portion outside the measuring container is formed of a metal tube,
Thin metal rod is extended upward from the bottom side of the metering vessel are passed through the ceiling portion of the weighing container, further, one of the metal rod and the metal tube as the anode or the cathode, while the cathode or anode The present invention is to provide a sample liquid collecting apparatus which constitutes the sensor by being electrically connected to corresponding contacts.

[Procedure amendment 4]

[Document name to be amended] Statement

[Correction target item name] 0007

[Correction method] Change

[Correction contents]

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, described in detail an embodiment of the present invention applied to the culture preparative apparatus according to FIG 1 and FIG 2. Figure 1 shows the preparative culture partial device as a device preparative sample liquid fraction. As shown in FIG. 1, the culture solution sorting apparatus 1 is configured such that a long and narrow cylindrical closed glass container is used as a measurement container 2, and a culture solution tank (a sample solution tank) for storing a culture solution in an upper portion of the measurement container 2. ) 3 communicates with the bottom side through a first transport pipe 4 made of silicon tube, and an elongated discharge pipe section 5 is provided at the bottom of the measuring container 2.
And a second transport pipe 6 made of a silicon tube is connected to the discharge pipe section 5, and the discharge port 7 at the tip of the second transport pipe 6 is
It faces the inlet 9 of the test tube 8 as a sample liquid collecting container. Then, the culture solution stored in the culture solution tank 3 made of glass is sucked into the measuring container 2 by suction negative pressure,
After the measurement, pressurized air is supplied into the measuring container 2 to pressurize the liquid surface at a predetermined pressure, thereby transporting the culture solution from the measuring container 2 to the test tube 8.
, A first pinch valve 11 is attached to the second transport pipe 6, and a second pinch valve 12 is attached to the second transport pipe 6.
The transport pipe 4 and the second transport pipe 6 are configured to be openable and closable, respectively. Here, the first pinch valve 11 and the second pinch valve 12 are connected to the first transport pipe 4 and the second transport pipe 6.
This is a valve that is squeezed radially from the outside, closed by crushing, and opened by releasing the squeezing pressure. For this reason, the sterilization inside and outside the first transport pipe 4 and the second transport pipe 6 can be easily performed . Then, the metering container to the second upper and discharging the container atmosphere in weighing container 2, a tubular pipe connecting portion 13 for supplying pressurized air at a predetermined pressure in the metering container 2 is integrally formed And this pipe connection 1
3 is provided with a suction and discharge pipe 15 having a filter 14 for cleaning and sterilization.
8 and the pressurizing pump 19.

[Procedure amendment 5]

[Document name to be amended] Statement

[Correction target item name] 0026

[Correction method] Change

[Correction contents]

The fluid thus collected culture solution fraction <br/> apparatus according to this embodiment, merely due the the variation of the viscosity of the culture broth as closing point in time and sample solution of each pinch valve 11, 12 In addition to eliminating the effects of inertia,
Since the first transport pipe 4 is cleaned and the second transport pipe 8 is cleaned and dried to prepare for the next collection of the culture solution, the reliability of automatic collection of the culture solution of a constant quality is improved as much as possible. be able to.

[Procedure amendment 6]

[Document name to be amended] Statement

[Correction target item name] 0027

[Correction method] Change

[Correction contents]

In the present embodiment, a suction pump 18 and a pressure pump 19 are provided,
However, it is also possible to configure the supply and discharge pump 36 and one filter 14,
The present invention includes such a configuration. In addition, when it is necessary to control the temperature of the culture solution sorting device 1 depending on the type of microorganism or the properties of the sample solution, the culture solution sorting device 1 is naturally installed in a temperature-controlled refrigerator such as a refrigerator. Things.

[Procedure amendment 7]

[Document name to be amended] Statement

[Correction target item name] 0030

[Correction method] Change

[Correction contents]

The invention according to claim 2 is characterized in that one end of the first transport pipe extending from the outlet inside the measuring container to the exposed portion outside the measuring container is made of a metal tube, and the thin metal rod is connected to the metal pipe.
Extending upward from the bottom side of the measuring container and penetrating the ceiling of the measuring container, further, one of the metal rod and the metal tube as an anode or a cathode, the other as a cathode or an anode,
By electrically connecting to each corresponding contact,
Since the sensor is configured and the metal pipe portion is used as it is as a part of the sensor and all the liquid levels above the fixed liquid level are sensed, the detection accuracy can be greatly improved. Further, it is excellent in cleanliness and sterility, has a very small influence on the volume of the measuring container, and has a simple structure and low cost.

[Procedure amendment 8]

[Document name to be amended] Statement

[Correction target item name] Fig. 1

[Correction method] Change

[Correction contents]

1 is a configuration diagram of a culture preparative apparatus according to an embodiment of the sample liquid preparative apparatus of the present invention.

Claims (2)

[Claims] 1. A first transport pipe communicates between an upper side in a sealed measuring container and a bottom side in a sample liquid tank for storing a sample liquid, and the sample liquid is connected to the bottom of the measuring container from the measuring container. A second transport pipe for transporting the sample fluid to the collection container; a first pinch valve and a second pinch valve for opening and closing the first transport pipe and the second transport pipe, respectively; A sensor for detecting the liquid level of the sample liquid in the measuring container and outputting a signal when the liquid level reaches a fixed level; and a suction pump and a measuring container for discharging the atmosphere in the measuring container from the upper part of the measuring container. A sample liquid fractionating device, which is connected via a filter to a pressurizing pump that pressurizes the liquid level of the sample liquid in the sample container, wherein the outlet in the measuring container of the first transport pipe is placed at a liquid level of a fixed amount of the sample liquid. Contact position, and the sample start signal by the timer Is input, the first pinch valve is opened, the second pinch valve is closed, and the suction pump is operated until a signal is output from the sensor to collect a sample liquid in the measuring container. ,
When a signal is output from the sensor, the suction pump is stopped, the first pinch valve is closed, and the second pinch valve is opened. A sample liquid collecting apparatus, comprising a controller for transferring a sample liquid to a collection container. 2. One end of the first transport pipe extending from the inner outlet of the measuring container to the exposed portion outside the measuring container is formed of a metal tube, and a thin metal rod extends upward from the bottom side in the closed container. And let the ceiling part of the measuring container penetrate, and further, by electrically connecting one of the metal rod and the metal tube as an anode or a cathode, and the other as a cathode or an anode to corresponding contacts, respectively. The sample liquid collecting apparatus according to claim 1, wherein the sensor is configured.