CN113403199A - Online real-time detection system and method for biological sample - Google Patents

Abstract

The application provides an online real-time detection system and a detection method for a biological sample. The application provides a biological sample on-line real-time detection system includes: a culture vessel; the sampling pipe fitting is used for sampling a sample to be detected from the culture container to the quantitative component; the quantitative component comprises a quantitative structure, and the quantitative structure is used for determining the amount of the sample to be detected, which is sampled from the culture container to the quantitative component; the sampling host comprises a main pump, wherein the main pump is used for driving the sample to be detected to the quantitative component from the culture container and driving the sample to be detected determined by the quantitative structure to the analyzer; the analyzer is used for receiving the sample to be detected and analyzing the sample to be detected to obtain an analysis result; the feeding assembly is connected to a feeding hole of the culture container through a pipeline; the control part, the main pump, the analyzer and the feeding assembly are in signal connection with the control part, and the control part is used for obtaining an analysis result and controlling the feeding assembly to feed materials to the culture container according to the analysis result.

Description

Online real-time detection system and method for biological sample

Technical Field

The application belongs to the technical field of biological sample detection, and particularly relates to a biological sample online real-time detection system and method.

Background

In the prior art, in industries such as microbial culture and cell culture, when a culture medium is detected, a sample is firstly manually taken out of a culture container, then the sample is put into a centrifuge for centrifugal treatment, then a supernatant is taken out, and the supernatant is manually transferred to an analysis device for detection. And after the detection result is obtained through detection, judging whether the culture medium in the container is supplemented, and if the culture medium needs to be supplemented, manually controlling a supplementing device to supplement the culture medium.

Specifically, in the industries of microbial culture, cell culture and the like, a culture tank made of glass or stainless steel is adopted at present, and the size of the tank body is from several milliliters to several hundred liters. The artifical culture medium sample that takes out in the culture tank, need let in high-pressure steam to the sample connection before the sample to carry out 30 minutes sterilizations, then open the sample connection and emit anterior segment sample, and get the back end sample and carry out quick encapsulation. After sampling, the sampling port was purged with high-pressure steam for 30 minutes for sterilization. And transferring the taken sample to a centrifuge for centrifugal separation, and taking the supernatant to an analysis device for detection and analysis. Typically, the centrifugation apparatus and the analysis apparatus are located in other laboratories, with inevitable intervals in space and time. And after obtaining the test result of the sample, an operator judges whether to feed according to calculation, and under the condition of feeding requirement, the operator manually controls feeding equipment to feed quantitatively.

The above prior art has the following technical drawbacks:

firstly, the sterilization operation before and after sampling is complicated and takes long time, and the condition of high sampling frequency requirement cannot be met;

secondly, the whole detection process comprises manual sampling, centrifugation, detection and feeding, the consumed time is long, the component content of the detected item in the culture tank is continuously changed, and the content of the detected item in the culture tank is possibly changed when the feeding amount is calculated, so that the feeding cannot achieve the expected effect;

thirdly, the manual sampling amount cannot be accurately controlled, and the repeated sampling and monitoring cannot be realized for the cell culture industry with less culture medium;

fourthly, the whole existing detection process needs manual operation, and a large amount of labor cost needs to be consumed.

Disclosure of Invention

An object of the embodiments of the present application is to provide an online real-time detection system and a detection method for a biological sample, which have the advantages of accurate sampling, simplified process and integrated control.

In order to achieve the purpose, the technical scheme adopted by the application is as follows: provided is a biological sample online real-time detection system, which comprises:

the culture container is used for accommodating a sample to be detected;

the first end of the sampling pipe fitting is used for extending into the position below the liquid level of the sample to be detected, the second end of the sampling pipe fitting is connected with the quantifying component, and the sampling pipe fitting is used for enabling the sample to be detected to be sampled from the culture container to the quantifying component;

the quantitative assembly comprises a quantitative structure, the first end of the quantitative structure is connected to the second end of the sampling pipe fitting, the second end of the quantitative structure is connected to the sampling host, and the quantitative structure is used for determining the amount of the sample to be detected, which is sampled from the culture container to the quantitative assembly;

the sampling host comprises a main pump, the main pump is connected between the quantitative component and the analyzer through a pipeline, and the main pump is used for driving a sample to be detected to the quantitative component from the culture container and driving the sample to be detected determined by the quantitative structure to the analyzer;

the analyzer is used for receiving the sample to be detected and analyzing the sample to be detected to obtain an analysis result;

the feeding assembly is connected to the feeding hole of the culture container through a pipeline;

and the control part is used for acquiring the analysis result and controlling the feeding assembly to feed materials to the culture vessel according to the analysis result.

In one embodiment, the online real-time biological sample detection system further comprises a sample receiving assembly;

the sample receiving assembly comprises a receiving pool and a sample moving needle connected to the receiving pool, the receiving pool is connected to the main pump, and the sample moving needle is connected to the analyzer.

In one embodiment, the quantitative assembly further comprises a first three-way electromagnetic valve, a second three-way electromagnetic valve, a disinfection container and an air filter; wherein the content of the first and second substances,

the normally closed end of the first three-way electromagnetic valve is connected to the second end of the sampling pipe fitting, the normally open end of the first three-way electromagnetic valve is connected to the common end of the second three-way electromagnetic valve through a pipeline, and the common end of the first three-way electromagnetic valve is connected to the first end of the quantitative structure;

the normally closed end of the second three-way electromagnetic valve is connected to the disinfection container through a pipeline, the disinfection container is used for containing disinfectant, and the normally open end of the second three-way electromagnetic valve is connected to the air filter through a pipeline;

the first three-way electromagnetic valve and the second three-way electromagnetic valve are in signal connection with the control part.

In one embodiment, the dosing assembly further comprises a first liquid detector and a second liquid detector; wherein the content of the first and second substances,

the normally closed end of the second three-way electromagnetic valve is connected to the first port of the first liquid detection instrument through a pipeline, and the disinfection container is connected to the second port of the first liquid detection instrument through a pipeline;

the second end of the quantitative structure is connected to the first port of the second liquid detection instrument, and the main pump is connected to the second port of the second liquid detection instrument through a pipeline;

the first liquid detector and the second liquid detector are in signal connection with the control part.

In one embodiment, the sampling host further comprises a solenoid valve, the second end of the quantitative structure is connected to the normally closed end of the solenoid valve through a pipeline, the main pump is connected to the normally closed end of the solenoid valve through a pipeline, and the solenoid valve is in signal connection with the control part.

In one embodiment, the sampling host comprises at least two electromagnetic valves, and at least two normally closed ends of the at least two electromagnetic valves are connected to the main pump through pipelines;

the online real-time detection system for the biological sample comprises at least two sampling pipe fittings, at least two quantitative components and at least two material supplementing components; the at least two sampling pipe fittings, the at least two quantitative components and the at least two material supplementing components are connected in a one-to-one correspondence manner; at least two second ends of at least two quantitative structures in at least two quantitative assemblies are connected with at least two normally closed ends of at least two electromagnetic valves in a one-to-one correspondence mode.

In one embodiment, the sample receiving assembly further comprises at least one of a drain assembly, a water inlet assembly, and an overflow assembly; wherein the content of the first and second substances,

the drainage assembly comprises a drainage pipeline, a first driving pump and a first collecting container, wherein the first end of the drainage pipeline is connected to the receiving pool, the second end of the drainage pipeline is connected to the first collecting container, and the first driving pump is arranged on the drainage pipeline;

the overflow assembly comprises an overflow pipeline, a second driving pump and a second collecting container, wherein the first end of the overflow pipeline is connected to the receiving pool, the second end of the overflow pipeline is connected to the second collecting container, and the second driving pump is arranged on the overflow pipeline;

the water inlet assembly comprises a water inlet pipeline, a third driving pump and a pure water container, the pure water container is used for containing pure water, the first end of the water inlet pipeline is connected to the receiving pool, the second end of the water inlet pipeline is connected to the pure water container, and the third driving pump is arranged on the water inlet pipeline;

the first driving pump, the second driving pump and the third driving pump are all in signal connection with the control part.

In one embodiment, the sample receiving assembly further comprises a third liquid detector, the main pump is connected to a first port of the third liquid detector through a pipeline, the sample receiving pool is connected to a second port of the third liquid detector through a pipeline, and the third liquid detector is in signal connection with the control part.

In one embodiment, the sampling tube comprises a probe tube, a membrane tube and a cock; wherein the content of the first and second substances,

the first end of the probe tube extends into the culture container, and the second end of the probe tube is connected to the quantifying assembly through a hose; the rotary valve is arranged at one end of the hose close to the probe; the membrane tube is connected to the first end of the probe tube and used for extending below the liquid level of the sample to be detected.

In one embodiment, the feed assembly comprises a feed pipeline, a feed pump and a feed container, wherein the feed container is used for accommodating feed liquid; wherein the content of the first and second substances,

the first end of the feed supplement pipeline is connected to the feed inlet of the culture container, and the second end of the feed supplement pipeline is used for extending into the position below the liquid level of the feed supplement liquid; the feeding pump is arranged on the feeding pipeline and is in signal connection with the control part.

Compared with the prior art, the online real-time biological sample detection system provided by the application can determine the amount of a sample to be detected from a culture container to a quantitative component in real time through the combination of the control part and the quantitative component through the quantitative structure, drive the sample to be detected from the culture container to the quantitative component through the main pump in real time, drive the sample to be detected determined by the quantitative structure to an analyzer, analyze the sample to be detected in real time through the analyzer to obtain an analysis result, and control the material supplementing component to supplement materials to the culture container in real time according to the analysis result through the real-time control of the control system. Which has the following

Therefore, the online real-time biological sample detection system provided by the application has the beneficial effects that:

firstly, the sampling procedure is simplified, the time from sampling to material supplementing is shortened, and the problem that high-frequency and high-efficiency sampling cannot be met due to complicated sampling procedure and long time consumption in the prior art is solved;

secondly, the sampling quantity of a single time can be accurately controlled, and the problem that the sampling quantity cannot be accurate due to manual sampling in the prior art is solved;

and fourthly, the whole process is automatic and integrated, and the problem that in the prior art, the labor cost is high due to the fact that a large amount of labor is needed in the whole monitoring process is solved.

Another object of the present application is to provide a detection method of the above-mentioned online real-time biological sample detection system, which includes:

the main pump is started and operated by the control part, so that the main pump drives the sample to be detected in the culture container to the quantitative structure of the quantitative component through the sampling pipe fitting for quantification;

the main pump drives the sample to be detected quantified by the quantifying structure to an analyzer by the quantifying component;

the analyzer receives the sample to be detected and analyzes the sample to be detected to obtain an analysis result;

the control part obtains the analysis result by the analyzer and controls the feeding component to feed materials to the culture vessel according to the analysis result.

In one embodiment, before the main pump drives the sample to be tested in the culture container into the quantifying structure of the quantifying assembly through the sampling pipe to be quantified, the method further comprises the following steps:

the control portion controls a solenoid valve to open a normally closed end of the solenoid valve and close a normally open end of the solenoid valve;

the control portion controls a second three-way electromagnetic valve to open a normally closed end of the second three-way electromagnetic valve and close a normally open end of the second three-way electromagnetic valve;

the main pump drives the disinfectant in the disinfection container to the quantitative structure through the second three-way electromagnetic valve and the first three-way electromagnetic valve so as to carry out quantitative determination;

the quantitative structure quantifies the disinfectant, the first liquid detector obtains a quantitative completion signal and sends the quantitative completion signal to the control part, and the control part controls the second three-way electromagnetic valve according to the quantitative completion signal so as to close the normally closed end of the second three-way electromagnetic valve and open the normally open end of the second three-way electromagnetic valve;

the main pump drives the disinfectant quantified by the quantifying structure to the sample receiving assembly through the quantifying structure.

In one embodiment, after the main pump drives the disinfecting liquid quantified by the quantifying structure to the sample receiving assembly through the quantifying structure, before the main pump drives the sample to be detected in the culture container to the quantifying structure of the quantifying assembly through the sampling pipe for quantification, the method further includes:

the receiving pool of the sample receiving assembly receives the disinfectant and overflows, the third liquid detector obtains an overflow signal and sends the overflow signal to the control part, the control part controls the second driving pump to drive the disinfectant in the overflow part to the second collecting container according to the overflow signal, and the control part controls the electromagnetic valve according to the overflow signal to close the normally closed end of the electromagnetic valve and open the normally open end of the electromagnetic valve;

the control part controls a first driving pump to drive the disinfectant in the receiving tank to a first collecting container;

the control part controls the third driving pump to drive the pure water in the pure water container to the receiving pool, and the control part controls the second driving pump to drive the pure water in the receiving pool to the second collecting container.

In one embodiment, after the control portion controls the third driving pump to drive the pure water in the pure water container into the receiving tank, and the control portion controls the second driving pump to drive the pure water in the receiving tank into the second collection container, before the main pump drives the sample to be detected in the culture container into the quantitative structure of the quantitative component through the sampling pipe to perform quantitative determination, the method further includes:

the control portion controls the first three-way solenoid valve to open a normally closed end of the second three-way solenoid valve and close a normally open end of the second three-way solenoid valve.

In one embodiment, after the main pump drives the sample to be detected in the culture container into the quantifying structure of the quantifying assembly through the sampling pipe to perform the quantification, before the main pump drives the sample to be detected quantified by the quantifying structure to the analyzer from the quantifying assembly, the method further comprises:

the control portion controls the first three-way solenoid valve to close a normally closed end of the second three-way solenoid valve and open a normally open end of the second three-way solenoid valve.

Compared with the prior art, the biological sample online real-time detection method provided by the application has the advantages that the main pump and the material supplementing assembly are controlled by the control part, so that a sample to be detected in the culture container is driven into the quantitative structure of the quantitative assembly through the sampling pipe fitting to be quantified by the main pump, the sample to be detected quantified by the quantitative structure can be driven to the analyzer by the quantitative assembly, the analyzer receives the sample to be detected and analyzes the sample to be detected to obtain an analysis result, and the control part obtains the analysis result by the analyzer and controls the material supplementing assembly to supplement materials to the culture container according to the analysis result.

Therefore, the online real-time detection method for the biological sample has the advantages that:

firstly, the sampling procedure is simplified, the time from sampling to material supplementing is shortened, and the problem that high-frequency and high-efficiency sampling cannot be met due to complicated sampling procedure and long time consumption in the prior art is solved;

secondly, the sampling quantity of a single time can be accurately controlled, and the problem that the sampling quantity cannot be accurate due to manual sampling in the prior art is solved;

and fourthly, the whole process is automatic and integrated, and the problem that in the prior art, the labor cost is high due to the fact that a large amount of labor is needed in the whole monitoring process is solved.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.

Fig. 1 is a first schematic view of an online real-time biological sample detection system provided in an embodiment of the present application;

fig. 2 is a second schematic view of an online real-time biological sample detection system provided in an embodiment of the present application.

Wherein, in the figures, the respective reference numerals:

10. a culture vessel; 20. a sampling tube; 30. a dosing assembly; 40. a sampling host; 50. a sample receiving assembly; 60. an analyzer; 70. a feed supplement assembly;

201. a probe tube; 202. a membrane tube; 203. turning a valve; 204. a hose;

301. a first three-way solenoid valve; 302. a second three-way solenoid valve; 303. sterilizing the container; 304. an air filter; 305. a first liquid detector; 306. a second liquid detector;

401. a main pump; 402. an electromagnetic valve;

501. a drainage assembly; 502. an overflow assembly; 503. a water intake assembly; 504. a receiving tank; 505. a sample transferring needle; 506. a third liquid detector; 507. a stirring device;

501a, a drainage pipeline; 501b, a first driving pump; 501c, a first collection container;

502a, an overflow conduit; 502b, a second drive pump;

503a, a water inlet pipeline; 503b, a third drive pump; 503c, a pure water container;

601-a display;

701. a material supplementing pipeline; 702. a feed pump; 703. a feeding container;

a. a normally closed end; b. a normally open end; c. a common terminal.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the present application clearer, the present application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.

It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings that is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the present application.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

The on-line real-time detection system and the detection method for biological samples provided by the embodiments of the present application will now be described.

Referring to fig. 1, an online real-time detection system for a biological sample provided in an embodiment of the present application includes: a culture container 10 for accommodating a sample to be tested; the sampling pipe fitting 20 is used for extending into the position below the liquid level of the sample to be detected, the second end of the sampling pipe fitting 20 is connected to the quantifying component 30, and the sampling pipe fitting 20 is used for enabling the sample to be detected to be sampled from the culture container 10 to the quantifying component 30; a quantitative assembly 30 including a quantitative structure, a first end of the quantitative structure being connected to the second end of the sampling pipe 20, a second end of the quantitative structure being connected to the sampling host 40, the quantitative structure being used for determining the amount of the sample to be detected sampled from the culture container 10 to the quantitative assembly 30; the sampling host 40 comprises a main pump 401, the main pump 401 is connected between the quantifying assembly 30 and the analyzer 60 through a pipeline, the main pump 401 is used for driving the sample to be detected from the culture container 10 to the quantifying assembly 30 and driving the sample to be detected determined by the quantifying structure to the analyzer 60; an analyzer 60 for receiving a sample to be detected and analyzing the sample to be detected to obtain an analysis result; a feeding assembly 70 connected to the feed inlet of the culture vessel 10 through a pipe; the control part, the main pump 401, the analyzer 60 and the feeding assembly 70 are all in signal connection with the control part, and the control part is used for obtaining the analysis result and controlling the feeding assembly 70 to feed the culture vessel 10 according to the analysis result.

Compared with the prior art, the online real-time biological sample detection system provided by the embodiment of the application determines the amount of the sample to be detected sampled from the culture container 10 to the quantitative component 30 in real time through the quantitative structure by combining the control part and the quantitative component 30, drives the sample to be detected from the culture container 10 to the quantitative component 30 in real time through the main pump 401, drives the sample to be detected determined by the quantitative structure to the analyzer 60, analyzes the sample to be detected in real time through the analyzer 60 to obtain an analysis result, and controls the feeding component 70 to feed the culture container 10 in real time according to the analysis result through the real-time control of the control system. Which has the following

Therefore, the online real-time biological sample detection system provided by the application has the beneficial effects that:

firstly, the sampling procedure is simplified, the time from sampling to material supplementing is shortened, and the problem that high-frequency and high-efficiency sampling cannot be met due to complicated sampling procedure and long time consumption in the prior art is solved;

secondly, the sampling quantity of a single time can be accurately controlled, and the problem that the sampling quantity cannot be accurate due to manual sampling in the prior art is solved;

and fourthly, the whole process is automatic and integrated, and the problem that in the prior art, the labor cost is high due to the fact that a large amount of labor is needed in the whole monitoring process is solved.

In the embodiment of the present application, the control portion may be independent of other components, or the control portion is integrated in the sampling host 40, or the control portion is integrated in the analyzer 60.

In the embodiment of the present application, the sampling host 40 and the analyzer 60 may be provided independently, or both may be integrated. When the sampling main body 40 and the analyzer 60 are integrated, the control portion is integrated in the integrated structure.

In one embodiment, the sampling tube 20 comprises a probe 201, a membrane tube 202 and a stopcock 203; wherein a first end of the probe 201 extends into the culture container 10, and a second end of the probe 201 is connected to the quantitative assembly 30 through the flexible tube 204. Wherein, the wall of the probe 201 is sealed with the culture container 10 by a sealing link, for example, by a sealing ring. The hose 204 is preferably a teflon tube, the rotary valve 203 is disposed at one end of the hose 204 close to the probe 201, the rotary valve 203 is a rotary valve for controlling the on/off of the hose 204, and the type of the rotary valve can be arbitrarily selected, for example, the rotary valve 203 can be selected as a manual rotary valve 203. Of course, in other embodiments, the valve 203 may be eliminated.

The membrane tube 202 is connected to a first end of the probe tube 201, and the membrane tube 202 is used for extending below the liquid level of the sample to be detected. Wherein, the membrane tube 202 adopts a micropore ceramic tube, wherein, the diameter of the micropore is more than or equal to 0.2 μm. The diameter of the micropores can be as small as 0.2 μm, and the diameter of the common bacteria is about 0.5 μm, so that the bacteria can be effectively isolated, and the filtration of the culture medium can be efficiently realized. Of course, other materials or other shapes of the membrane tube 202 may be used.

In one embodiment, the dosing assembly 30 further comprises a first three-way solenoid valve 301, a second three-way solenoid valve 302, a sterilization container 303, an air filter 304; wherein, the normally closed end a of the first three-way electromagnetic valve 301 is connected to the second end of the sampling pipe fitting 20, the normally open end b of the first three-way electromagnetic valve 301 is connected to the common end c of the second three-way electromagnetic valve 302 through a pipeline, and the common end c of the first three-way electromagnetic valve 301 is connected to the first end of the quantitative structure; the normally closed end a of the second three-way electromagnetic valve 302 is connected to a disinfection container 303 through a pipeline, the disinfection container 303 is used for containing disinfection liquid, and the normally open end b of the second three-way electromagnetic valve 302 is connected to an air filter 304 through a pipeline; the first three-way solenoid valve 301 and the second three-way solenoid valve 302 are in signal connection with the control part. The embodiment of the application adopts the three-way electromagnetic valve, so that the real-time operation of the detection system can be conveniently controlled, and the real-time degree and the accuracy of control are improved.

Wherein, the quantitative structure adopts a pipe with small inner diameter, and the quantitative structure realizes the quantitative determination by matching with the liquid detection device with fixed length. In other alternatives, the quantification may be achieved by timing or weighting, which is well known in the art and will not be described herein.

Wherein, the filter element of the air filter 304 is made of waterproof and breathable material with the diameter of the micropore of 0.2 μm, and can effectively isolate bacteria.

In one embodiment, the dosing assembly 30 further comprises a first fluid detector 305 and a second fluid detector 306; wherein, the normally closed end a of the second three-way solenoid valve 302 is connected to the first port of the first liquid detector 305 through a pipeline, and the disinfection container 303 is connected to the second port of the first liquid detector 305 through a pipeline; the second end of the quantitative structure is connected to the first port of the second liquid detector 306, and the main pump 401 is connected to the second port of the second liquid detector 306 through a pipeline; the first liquid detector 305 and the second liquid detector 306 are in signal connection with the control part.

In one embodiment, the sampling host 40 further comprises a solenoid valve 402, the second end of the quantitative structure is connected to the normally closed end a of the solenoid valve through a pipeline, the main pump 401 is connected to the normally closed end a of the solenoid valve through a pipeline, and the solenoid valve is in signal connection with the control part.

As shown in fig. 2, in an embodiment, the sampling main machine 40 includes at least two solenoid valves 402, and at least two normally closed ends a of the at least two solenoid valves 402 are connected to the main pump 401 through pipes; the online real-time detection system for the biological samples comprises at least two sampling pipes 20, at least two quantitative assemblies 30 and at least two material supplementing assemblies 70; the at least two sampling pipes 20, the at least two quantitative assemblies 30 and the at least two feeding assemblies 70 are correspondingly connected one by one; at least two second ends of at least two dosing structures in the at least two dosing assemblies 30 and at least two normally closed ends a of the at least two solenoid valves 402 are connected in a one-to-one correspondence.

In this embodiment, the sampling main unit 40 is provided with four channels, each of which is provided with a solenoid valve 402 for individually controlling the flow path, and the four channels are matched with the independent sampling pipe 20, the quantitative component 30, the supplementary material component 70 and the culture vessel 10, and the other ends of the four channels are gathered to the pump pipe of the main pump 401 through a plurality of three-way joints.

In one embodiment, the online real-time biological sample detection system further comprises a sample receiving assembly 50; the sample reception unit 50 includes a reception cell 504 and a transfer pin 505 connected to the reception cell 504, the reception cell 504 being connected to the main pump 401, and the transfer pin 505 being connected to the analyzer 60.

In the embodiment, the sample receiving member 50 may be integrated into the analyzer 60 or may be separately configured outside the analyzer 60. Preferably, in the present application, the sample receiving assembly 50 is integrated into the analyzer 60.

In one embodiment, sample receiving element 50 further comprises at least one of a drain element 501, a water inlet element 503, and an overflow element 502. In the present application, sample receiving member 50 preferably includes a drain member 501, a water inlet member 503, and an overflow member 502.

The drainage assembly 501 comprises a drainage pipeline 501a, a first driving pump 501b and a first collection container 501c, wherein a first end of the drainage pipeline 501a is connected to the receiving tank 504, a second end of the drainage pipeline 501a is connected to the first collection container 501c, and the first driving pump 501b is arranged on the drainage pipeline 501 a; the overflow assembly 502 includes an overflow pipe 502a, a second driving pump 502b, and a second collection container, a first end of the overflow pipe 502a is connected to the receiving tank 504, a second end of the overflow pipe 502a is connected to the second collection container, and the second driving pump 502b is disposed on the overflow pipe 502 a; the water inlet assembly 503 comprises a water inlet pipe 503a, a third driving pump 503b and a pure water container 503c, the pure water container 503c is used for containing pure water, a first end of the water inlet pipe 503a is connected to the receiving tank 504, a second end of the water inlet pipe 503a is connected to the pure water container 503c, and the third driving pump 503b is arranged on the water inlet pipe 503 a; the first driving pump 501b, the second driving pump 502b and the third driving pump 503b are all in signal connection with the control part.

In the embodiment of the present application, the first collection container 501c and the second collection container are common, but they may be provided separately.

In one embodiment, the sample receiving assembly 50 further comprises a third fluid detector 506, the main pump 401 is connected to a first port of the third fluid detector 506 through a pipeline, the sample receiving cell 504 is connected to a second port of the third fluid detector 506 through a pipeline, and the third fluid detector 506 is in signal connection with the control part.

In one embodiment, the feed assembly 70 comprises a feed pipeline 701, a feed pump 702 and a feed container 703, wherein the feed container 703 is used for accommodating a feed liquid; wherein, the first end of the feeding pipeline 701 is connected to the feeding port of the culture container 10, and the second end of the feeding pipeline 701 is used for extending below the liquid level of the feeding liquid; the feeding pump 702 is arranged on the feeding pipeline 701, and the feeding pump 702 is in signal connection with the control part.

In the embodiment of the present application, one feeding pump 702 is provided for each feeding channel, and in other alternatives, the feeding pump 702 is not provided, the driving feeding is realized by the main pump 401, or a plurality of feeding pumps 702 are provided in one feeding channel.

Another object of the present application is to provide a detection method of the above-mentioned online real-time biological sample detection system, which includes:

the main pump 401 is started and operated by the control part, so that the main pump 401 drives the sample to be detected in the culture vessel 10 into the quantitative structure of the quantitative component 30 through the sampling pipe member 20 for quantification;

the main pump 401 drives the sample to be detected quantified by the quantifying structure from the quantifying assembly 30 to the analyzer 60;

the analyzer 60 receives the sample to be detected and analyzes the sample to be detected to obtain an analysis result;

the control part obtains the analysis result from the analyzer 60 and controls the feeding assembly 70 to feed the culture vessel 10 according to the analysis result.

In one embodiment, before the main pump 401 drives the sample to be tested in the culture container 10 to the quantitative structure of the quantitative component 30 through the sampling pipe 20 for quantification, the method further comprises:

the control portion controls the solenoid valve 402 to open the normally closed end a of the solenoid valve 402 and close the normally open end b of the solenoid valve 402;

the control portion controls the second three-way solenoid valve 302 to open the normally closed end a of the second three-way solenoid valve 302 and close the normally open end b of the second three-way solenoid valve 302;

the main pump 401 drives the disinfectant in the disinfection container 303 to a quantitative structure through the second three-way electromagnetic valve 302 and the first three-way electromagnetic valve 301 for quantitative determination;

the quantifying structure quantifies the disinfectant, the first fluid detector 305 obtains a quantifying completion signal and sends the quantifying completion signal to the control part, and the control part controls the second three-way electromagnetic valve 302 according to the quantifying completion signal to close the normally closed end a of the second three-way electromagnetic valve 302 and open the normally open end b of the second three-way electromagnetic valve 302;

the main pump 401 drives the disinfectant solution dosed by the dosing structure from the dosing structure to the sample receiving assembly 50.

In one embodiment, after the main pump 401 drives the disinfectant liquid metered by the metering structure to the sample receiving assembly 50, before the main pump 401 drives the sample to be tested in the culture container 10 to the metering structure of the metering assembly 30 through the sampling pipe 20 for metering, the method further comprises:

the receiving pool 504 of the sample receiving assembly 50 receives the disinfecting liquid and overflows, the third liquid detector 506 acquires an overflow signal and sends the overflow signal to the control part, the control part controls the second driving pump 502b to drive the disinfecting liquid in the overflow part to the second collecting container according to the overflow signal, and the control part controls the electromagnetic valve 402 according to the overflow signal to close the normally closed end a of the electromagnetic valve 402 and open the normally open end b of the electromagnetic valve 402;

the control part controls the first driving pump 501b to drive the disinfectant in the receiving pool 504 to the first collecting container 501 c;

the control section controls the third drive pump 503b to drive pure water in the pure water container 503c to the receiving tank 504, and the control section controls the second drive pump 502b to drive pure water in the receiving tank 504 to the second collection container.

In one embodiment, after the control part controls the third driving pump 503b to drive the pure water in the pure water container 503c into the receiving tank 504, and the control part controls the second driving pump 502b to drive the pure water in the receiving tank 504 into the second collection container, before the main pump 401 drives the sample to be detected in the culture container 10 into the quantitative structure of the quantitative component 30 through the sampling pipe 20 for quantitative determination, the method further includes:

the control portion controls the first three-way solenoid valve 301 to open the normally closed end a of the second three-way solenoid valve 302 and close the normally open end b of the second three-way solenoid valve 302.

In one embodiment, after the main pump 401 drives the sample to be tested in the culture container 10 to the quantitative structure of the quantitative component 30 through the sampling pipe 20 for quantification, before the main pump 401 drives the sample to be tested quantified by the quantitative structure to the analyzer 60 from the quantitative component 30, the method further comprises:

the control portion controls the first three-way solenoid valve 301 to close the normally closed end a of the second three-way solenoid valve 302 and open the normally open end b of the second three-way solenoid valve 302.

The sampling tube 20 may be sterilized at a high temperature before being placed in the culture vessel 10, or the sampling tube 20 and the cock 203 at the end thereof may be sterilized in situ after the sampling tube 20 is placed in the culture vessel 10 in synchronization with the sterilization process of the culture vessel 10. Furthermore, all the lines of the feed pump 702 are also sterilized at high temperature.

Taking a single channel as an example, the online real-time detection method of the biological sample specifically comprises the following steps:

first, disinfecting, cleaning and emptying actions

After the system is started, the detection system automatically performs disinfecting, cleaning and emptying actions, the control part sends an instruction according to the starting signal, controls to open the normally closed end a and close the normally open end b of the electromagnetic valve 402 corresponding to the channel, and controls to open the normally closed end a and close the normally open end b of the second three-way electromagnetic valve 302. After that, the control part controls the main pump 401 to start to rotate anticlockwise, so as to drive the disinfectant in the disinfection container 303 to enter the quantitative structure of the quantitative component 30, the quantitative structure realizes the quantitative determination of the entering disinfectant through a fixed-length pipe with a certain inner diameter and matching with the second liquid detector 306, the default quantitative determination is 1mL, and the quantitative determination can be adjusted according to the situation.

When the metering is finished, the control part controls to close the normally closed end a of the second three-way electromagnetic valve 302 and open the normally open end b thereof, the main pump 401 continues to rotate, and the liquid tail of the disinfectant is communicated with the atmosphere through the air filter 304. Under the continuous rotation of the main pump 401, the disinfectant solution which is quantitatively taken out is pumped into the receiving tank 504 in the analyzer 60, and meanwhile, the flow path near the main pump 401 is emptied.

When the disinfectant reaches the third liquid detector 506 inside the analyzer 60, it indicates that the receiving tank 504 overflows, the second driving pump 502b inside the analyzer 60 starts to rotate continuously, and excess disinfectant overflowing after filling the receiving tank 504 is pumped into the second collection container.

When the third liquid detector 506 detects that the liquid is changed from liquid to no liquid, the second driving pump 502b operates for a short time and stops rotating. While the control portion controls to close the normally closed end a and open the normally open end b of the solenoid valve 402. At this time, the stirring device 507 in the analyzer 60 starts stirring for a predetermined time to perform stirring, disinfecting and cleaning in the receiving tank 504.

Then, the control section controls the first drive pump 501b in the analyzer 60 to discharge the disinfecting liquid in the receiving tank 504 into the first collection container 501c, and after a set time has elapsed, the control section controls and stops the rotation of the first drive pump 501 b. The control section controls the third driving pump 503b to pump pure water into the receiving tank 504, and controls the second driving pump 502b to rotate to pump pure water in the receiving tank 504 into the second collection container. After the set time has elapsed, the control portion controls and stops the third driving pump 503b, at which time the control portion controls the second driving pump 502b to stop operating for a short time. Then, the control section controls the first drive pump 501b to start rotating to drain the receiving tank 504, and then, the standby state is performed.

Second, on-line sampling action

And after the set time is reached, online sampling is carried out, the control part controls to open the normally closed end a of the first three-way electromagnetic valve 301 and simultaneously open the normally closed end a of the first three-way electromagnetic valve 301, and simultaneously the control part starts the main pump 401 to start running, so that the sample to be detected in the culture container 10 is pumped into the quantitative structure of the quantitative component 30 through the membrane tube 202 for quantification.

When the liquid head filtered by the sampling pipe 20 reaches the second liquid detector 306 of the quantitative component 30, the accurate quantitative determination of the sample is completed, and the default is 1 mL. At this time, the control part controls to close the normally closed end a of the first three-way electromagnetic valve 301 and simultaneously open the normally open end b of the first three-way electromagnetic valve 301, and at this time, the liquid tail of the sample to be detected is communicated with the air through the air filter 304.

The sample to be tested is pumped into a receiving well 504 inside the analyzer 60 during continuous operation of the main pump 401. When the liquid head of the sample to be detected reaches the third liquid detector 506 in the analyzer 60, the second driving pump 502b on the overflow pipe 502a starts to operate continuously, and after the sample fills the receiving tank 504, the excess sample is pumped away to prevent the sample from overflowing into the inside of the analyzer to form pollution. When the sample liquid tail in the pipeline is separated from the third liquid detector 506, the control part controls the main pump 401 to stop running, simultaneously closes the normally closed port a of the electromagnetic valve 402 and stops the running of the second driving pump 502b, and finishes the sampling action to wait for the sample moving needle 505 to move the sample into the receiving pool 504.

Third, analyze the movements

After the on-line sampling is completed, the sample moving needle 505 enters the receiving cell 504 to perform quantitative sampling, and the sample is moved to the testing position of the analyzer 60 to perform an analysis test. After the analysis is completed, the test result can be displayed on the display 601 and printed by the printer at the end of the analyzer 60, and finally, the sterilization, cleaning and emptying actions are performed on the whole system.

Four, reverse control action

Before use, the user can set the desired control concentration value of the measured parameter, and input the concentration value of the feed liquid and the initial liquid loading of the culture tank. When the sampling test result is obtained, the control part judges whether to feed the culture tank according to the expected control parameters set by the user. If the material is required to be supplemented, the control part automatically and accurately calculates the amount of the material to be supplemented according to the change of various parameters of the material supplement, and the analyzer 60 sends a signal to the control part, so that the material supplement pump 702 is controlled to quantitatively add the material supplement liquid into the culture container 10, and the quantitative control of the measured parameters in the culture container 10 is automatically realized.

Compared with the prior art, the online real-time detection method for the biological sample provided by the application has the advantages that the main pump 401 and the feeding component 70 are controlled by the control part, so that the main pump 401 drives the sample to be detected in the culture container 10 to the quantitative structure of the quantitative component 30 through the sampling pipe fitting 20 for quantification, the sample to be detected quantified by the quantitative structure can be driven to the analyzer 60 through the quantitative component 30, the analyzer 60 receives the sample to be detected and analyzes the sample to be detected to obtain an analysis result, and the control part obtains the analysis result through the analyzer 60 and controls the feeding component 70 to feed the culture container 10 according to the analysis result.

The above description is only exemplary of the present application and should not be taken as limiting the present application, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims (15)

1. An online real-time biological sample detection system, comprising:

a culture container (10) for containing a sample to be tested;

the sampling pipe fitting (20), a first end of the sampling pipe fitting (20) is used for extending below the liquid level of the sample to be detected, a second end of the sampling pipe fitting (20) is connected to the quantifying component (30), and the sampling pipe fitting (20) is used for enabling the sample to be detected to be sampled from the culture container (10) to the quantifying component (30);

the quantitative assembly (30) comprises a quantitative structure, the first end of the quantitative structure is connected to the second end of the sampling pipe fitting (20), the second end of the quantitative structure is connected to a sampling host (40), and the quantitative structure is used for determining the amount of the sample to be detected, which is sampled from the culture container (10) to the quantitative assembly (30);

the sampling host (40) comprises a main pump (401), the main pump (401) is connected between the quantifying assembly (30) and the analyzer (60) through a pipeline, and the main pump (401) is used for driving the sample to be detected from the culture container (10) to the quantifying assembly (30) and driving the sample to be detected determined by the quantifying structure to the analyzer (60);

the analyzer (60) is used for receiving the sample to be detected and analyzing the sample to be detected to obtain an analysis result;

a feed supplement assembly (70) connected to the feed inlet of the culture vessel (10) by a pipe;

a control part, wherein the main pump (401), the analyzer (60) and the feeding assembly (70) are in signal connection with the control part, and the control part is used for obtaining the analysis result and controlling the feeding assembly (70) to feed to the culture vessel (10) according to the analysis result.

2. The online real-time biological sample detection system of claim 1, wherein:

the online real-time detection system for the biological sample further comprises a sample receiving component (50);

the sample receiving assembly (50) comprises a receiving pool (504) and a sample transferring needle (505) connected to the receiving pool (504), the receiving pool (504) is connected to the main pump (401), and the sample transferring needle (505) is connected to the analyzer (60).

3. The online real-time biological sample detection system of claim 1 or 2, wherein:

the quantitative component (30) further comprises a first three-way electromagnetic valve (301), a second three-way electromagnetic valve (302), a disinfection container (303) and an air filter (304); wherein the content of the first and second substances,

the normally closed end (a) of the first three-way electromagnetic valve (301) is connected to the second end of the sampling pipe fitting (20), the normally open end (b) of the first three-way electromagnetic valve (301) is connected to the common end (c) of the second three-way electromagnetic valve (302) through a pipeline, and the common end (c) of the first three-way electromagnetic valve (301) is connected to the first end of the quantitative structure;

the normally closed end (a) of the second three-way electromagnetic valve (302) is connected to the disinfection container (303) through a pipeline, the disinfection container (303) is used for containing disinfection liquid, and the normally open end (b) of the second three-way electromagnetic valve (302) is connected to the air filter (304) through a pipeline;

the first three-way electromagnetic valve (301) and the second three-way electromagnetic valve (302) are in signal connection with the control part.

4. The online real-time biological sample detection system of claim 3, wherein:

the dosing assembly (30) further comprises a first liquid detector (305) and a second liquid detector (306); wherein the content of the first and second substances,

the normally closed end (a) of the second three-way solenoid valve (302) is connected to a first port of the first liquid detector (305) through a pipeline, and the disinfection container (303) is connected to a second port of the first liquid detector (305) through a pipeline;

the second end of the dosing structure is connected to a first port of the second liquid detector (306), and the main pump (401) is connected to a second port of the second liquid detector (306) through a pipeline;

the first liquid detector (305) and the second liquid detector (306) are in signal connection with the control part.

5. The online real-time biological sample detection system of claim 1 or 2, wherein:

the sampling host (40) further comprises a solenoid valve (402), the second end of the quantitative structure is connected to the normally closed end (a) of the solenoid valve through a pipeline, the main pump (401) is connected to the normally closed end (a) of the solenoid valve (402) through a pipeline, and the solenoid valve (402) is in signal connection with the control part.

6. The online real-time biological sample detection system of claim 5, wherein:

the sampling main machine (40) comprises at least two electromagnetic valves (402), and at least two normally closed ends (a) of the at least two electromagnetic valves (402) are connected to the main pump (401) through pipelines;

the online real-time detection system for the biological samples comprises at least two sampling pipes (20), at least two quantitative assemblies (30) and at least two feeding assemblies (70); at least two sampling pipes (20), at least two quantitative assemblies (30) and at least two feeding assemblies (70) are connected in a one-to-one correspondence manner; at least two second ends of at least two of the dosing structures in at least two of the dosing assemblies (30) and at least two normally closed ends (a) of at least two of the solenoid valves (402) are connected in a one-to-one correspondence.

7. The online real-time biological sample detection system of claim 2, wherein:

the sample receiving assembly (50) further comprises at least one of a drainage assembly (501), a water inlet assembly (503) and an overflow assembly (502); wherein the content of the first and second substances,

the drainage assembly (501) comprises a drainage pipeline (501a), a first driving pump (501b) and a first collection container (501c), a first end of the drainage pipeline (501a) is connected to the receiving tank (504), a second end of the drainage pipeline (501a) is connected to the first collection container (501c), and the first driving pump (501b) is arranged on the drainage pipeline (501 a);

the overflow assembly (502) comprises an overflow pipe (502a), a second driving pump (502b) and a second collecting container, a first end of the overflow pipe (502a) is connected to the receiving tank (504), a second end of the overflow pipe (502a) is connected to the second collecting container, and the second driving pump (502b) is arranged on the overflow pipe (502 a);

the water inlet assembly (503) comprises a water inlet pipeline (503a), a third driving pump (503b) and a pure water container (503c), the pure water container (503c) is used for containing pure water, a first end of the water inlet pipeline (503a) is connected to the receiving pool (504), a second end of the water inlet pipeline (503a) is connected to the pure water container (503c), and the third driving pump (503b) is arranged on the water inlet pipeline (503 a);

the first driving pump (501b), the second driving pump (502b) and the third driving pump (503b) are all in signal connection with the control part.

8. The online real-time biological sample detection system of claim 7, wherein:

the sample receiving assembly (50) further comprises a third liquid detector (506), the main pump (401) is connected to a first port of the third liquid detector (506) through a pipeline, the sample receiving pool (504) is connected to a second port of the third liquid detector (506) through a pipeline, and the third liquid detector (506) is in signal connection with the control part.

9. The online real-time biological sample detection system of claim 1 or 2, wherein:

the sampling pipe fitting (20) comprises a probe pipe (201), a membrane pipe (202) and a rotary valve (203); wherein the content of the first and second substances,

a first end of the probe (201) extends into the culture container (10), and a second end of the probe (201) is connected to the quantitative assembly (30) through a hose (204); the rotary valve (203) is arranged at one end of the hose (204) close to the probe (201); the membrane tube (202) is connected to the first end of the probe tube (201), and the membrane tube (202) is used for extending into the position below the liquid level of the sample to be detected.

10. The online real-time biological sample detection system of claim 1 or 2, wherein:

the feed assembly (70) comprises a feed pipeline (701), a feed pump (702) and a feed container (703), wherein the feed container (703) is used for accommodating feed liquid; wherein the content of the first and second substances,

the first end of the feed supplement pipeline (701) is connected to the feed inlet of the culture container (10), and the second end of the feed supplement pipeline (701) is used for extending into the position below the liquid level of the feed supplement liquid; the feeding pump (702) is arranged on the feeding pipeline (701), and the feeding pump (702) is in signal connection with the control part.

11. A method for detecting a biological sample on-line real-time detection system according to any one of claims 1 to 10, wherein:

the main pump (401) is started and operated by the control part, so that the main pump (401) drives the sample to be detected in the culture container (10) into the quantitative structure of the quantitative component (30) through the sampling pipe fitting (20) for quantitative determination;

the main pump (401) drives the sample to be detected, which is quantified by the quantifying structure, to an analyzer (60) by the quantifying assembly (30);

the analyzer (60) receives the sample to be detected and analyzes the sample to be detected to obtain an analysis result;

the control part obtains the analysis result from the analyzer (60) and controls a feeding component (70) to feed the culture vessel (10) according to the analysis result.

12. The online real-time detection method of a biological sample according to claim 11, characterized in that:

before the main pump (401) drives the sample to be tested in the culture container (10) into the quantitative structure of the quantitative component (30) through the sampling pipe (20) for quantification, the method further comprises the following steps:

the control portion controls a solenoid valve (402) to open a normally closed end (a) of the solenoid valve (402) and close a normally open end (b) of the solenoid valve (402);

the control portion controls a second three-way solenoid valve (302) to open a normally closed end (a) of the second three-way solenoid valve (302) and close a normally open end (b) of the second three-way solenoid valve (302);

the main pump (401) drives the disinfectant in the disinfection container (303) to the quantitative structure through the second three-way electromagnetic valve (302) and the first three-way electromagnetic valve (301) for quantitative determination;

the quantitative structure quantifies the disinfectant, the first liquid detector (305) obtains a quantitative completion signal and sends the quantitative completion signal to the control part, and the control part controls the second three-way electromagnetic valve (302) according to the quantitative completion signal to close a normally closed end (a) of the second three-way electromagnetic valve (302) and open a normally open end (b) of the second three-way electromagnetic valve (302);

the main pump (401) drives the sterilizing fluid dosed by the dosing structure from the dosing structure to a sample receiving assembly (50).

13. The online real-time detection method of a biological sample according to claim 12, characterized in that:

after the main pump (401) drives the sterilizing fluid metered by the metering structure to a sample receiving assembly (50), before the main pump (401) drives the sample to be tested in the culture container (10) into the metering structure of the metering assembly (30) through the sampling pipe (20) for metering, the method further comprises:

a receiving pool (504) of the sample receiving assembly (50) receives the sterilizing liquid and overflows, a third liquid detector (506) acquires an overflow signal and sends the overflow signal to the control part, the control part controls a second driving pump (502b) to drive the sterilizing liquid of the overflow part to a second collecting container according to the overflow signal, and the control part controls the electromagnetic valve (402) to close a normally closed end (a) of the electromagnetic valve (402) and open a normally open end (b) of the electromagnetic valve (402) according to the overflow signal;

the control part controls a first driving pump (501b) to drive the disinfectant in the receiving pool (504) into a first collection container (501 c);

the control section controls a third drive pump (503b) to drive pure water in a pure water container (503c) to the receiving tank (504), and the control section controls the second drive pump (502b) to drive the pure water in the receiving tank (504) to a second collection container.

14. The online real-time detection method of a biological sample according to claim 13, characterized in that:

before the main pump (401) drives the sample to be detected in the culture container (10) into the quantitative structure of the quantitative assembly (30) through the sampling pipe (20) for quantification after the control part controls the third driving pump (503b) to drive the pure water in the pure water container (503c) into the receiving tank (504), and the control part controls the second driving pump (502b) to drive the pure water in the receiving tank (504) into the second collection container, the control part further comprises:

the control portion controls the first three-way solenoid valve (301) to open a normally closed end (a) of the second three-way solenoid valve (302) and close a normally open end (b) of the second three-way solenoid valve (302).

15. The online real-time detection method of a biological sample according to claim 14, characterized in that:

after the main pump (401) drives the sample to be detected in the culture container (10) into the quantifying structure of the quantifying assembly (30) through the sampling pipe (20) for quantifying, before the main pump (401) drives the sample to be detected quantified by the quantifying structure to the analyzer (60) by the quantifying assembly (30), the method further comprises the following steps:

the control portion controls the first three-way solenoid valve (301) to close a normally closed end (a) of the second three-way solenoid valve (302) and open a normally open end (b) of the second three-way solenoid valve (302).

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