JPS5983031A - Liquid sampling and transferring apparatus - Google Patents

Abstract

PURPOSE:To draw a sample liquid at every specified time interval and to make it possible to perform transferring, by providing a liquid withdrawing part, a liquid holding container part, a sucking and injecting part, and a control means, which monitors and controls the operation of a liquid sampling and transferring apparatus. CONSTITUTION:A liquid sampling and transferring apparatus is constituted by a liquid withdrawing part A, a liquid holding container part B, a sucking and injecting part C, and a control means, which monitors and controls the operation of the liquid sampling and transferring apparatus. The liquid withdrawing part A has the following devices; a constant quantity sampling tube 3, which contains a specified amount of a sample liquid from a blood vessel 1 of a living body or a sample holding container through a blood withdrawing tube 2 and a supplying port 3A; a syringe port 5A, which is connected to said tube 3; and the like. In this constitution, the sample substance can be withdrawn at every specified time interval, and the transferring can be performed.

Description

[Detailed description of the invention]

The present invention provides a liquid collection/transfer device that continuously transfers a predetermined amount of sample liquid to a measuring section of an analyzer when testing or analyzing sample liquids such as whole blood, plasma, and serum. Regarding. This type of device injects a uniform amount of sample liquid into the measuring section of an analyzer, etc., has good reproducibility, makes cleaning the injection system easy, and can perform a series of operations automatically and reliably. This is requested. Conventionally, such liquid collection has been performed by combining a syringe and a collection port such as an injection needle, inserting the liquid collection port into a blood vessel or sample liquid storage container each time, and moving the syringe manually or mechanically to collect the sample. A method has been adopted in which the liquid is drained, and after the liquid is collected, it is again injected or transferred into the measuring section of an analyzer or the like using a syringe. 7. 'C: I'o, this injection amount is the minimum per injection, which is about several tens of μ6. There is still a method for continuously collecting blood from a living body, and there is a hemodialysis device using an artificial kidney. The blood collection system of this device usually does not have a cleaning function. That is, the measuring section continuously measures 7. Although it is necessary to obtain c data, normally biological changes occur over time and are 7f long. Generally, it is sufficient to repeat intermittent i11+1 constants of several minutes, and the measurement results can be regarded as continuous data. Also,
Except for special cases where the above-mentioned liquid dialysis apparatus is used, continuous collection of specimen fluid naturally increases the amount of specimen fluid collected, which may pose a safety problem for the living body. In view of the above-mentioned points, the present invention solves the problems of the prior art, in which a predetermined amount of sample fluid connected to a living body and repeatedly collected at predetermined time intervals is transferred to a rolling wheel J'1. It is an object of the present invention to provide a liquid sampling transfer device that is capable of automatically cleaning the passage of a sample liquid. According to the present invention, such a purpose is achieved by providing a liquid collection tube for collecting a sample liquid, a quantitative sampling tube for sampling and storing a predetermined amount of the sample liquid, and a residual liquid suction tube connected to the quantitative sampling tube. a liquid sampling section having a sampling syringe connected thereto, an air pump connected to the quantitative sampling tube, and a washing liquid pump connected to the quantitative sampling tube in parallel with the air pump; a liquid reservoir portion having a primary transfer tube, and a liquid reservoir container into which the sample liquid contained in the quantitative sampling tube is transferred and stored by the air pump via the primary transfer tube; a suction/injection unit having a syringe that aspirates a predetermined amount of the sample liquid in the liquid storage container via a secondary transfer tube connected to the container and injects it into the measurement unit via the injection tube; After the predetermined amount of the sample liquid is injected into the 6+1 collection unit, the liquid storage container (718j6, and the suction/injection unit) are injected into the sample liquid path from the cleaning liquid pump. By providing a control means for sending out the cleaning liquid for cleaning, and further repeating the steps of sending air by the air pump and discharging the cleaning liquid continuously, An embodiment of the present invention will be explained in detail based on the drawings ψJ
do. FIG. 1 shows the total configuration of one embodiment of the present invention (4, IM+), and FIG. 2 shows the a=b operation timing chart of FIG. 1. The liquid/roller device hood is used, for example, for a closed-loop whole blood tail continuous automatic dish sugar analyzer.
Operation of the suction/injection part C and this liquid collection/transfer device'ff:
! ii. Control means for viewing and controlling the L (not shown in the figure)
It consists of The liquid sampling section A collects the sample liquid from a living body, rllll tube] or an unfilled sample container.
A blood sampling tube 2, this blood sampling tube 2; a fixed, 1t tumpling tube 3 that accommodates a predetermined amount of sample liquid through 3A, and a residual liquid in the residual liquid inlet 3B of this quantitative sampling tube 3. A sampling syringe 5 having a syringe barrel 5A and a movable syringe rod 5B is connected via a suction tube 6, and a sampling syringe 5 is connected to a residual liquid inlet 3B of a quantitative sampling tube 3 via a secondary air tube 7 and a primary air tube 12. It consists of an air pump LIM and a cleaning liquid bonder 9 connected to the residual liquid suction port 3B via a secondary air tube 7 and a cleaning liquid tube 10. Further, the large mouth valve 4 is provided in the liquid path of the blood collection tube 2, and the air opening valve 8 is provided in the secondary air tube 7.
established in Note that 31 and 32 are a blood collection end detection switch and a residual liquid detection switch provided on the sampling syringe. The naube connection part 35 is a T-shaped connecting pipe that connects the secondary air tube 7 to the primary air tube 2 and then to the cleaning liquid tube 0 via the reverse valve 34, and connects the air pump 11 and the cleaning liquid pump 9 in parallel. Deploy. In addition, the liquid reservoir section B is connected to the supply of the quantitative sampling tube 3 [(the primary transfer tube 14 which is connected to three people and transfers the sample liquid, washing liquid, and air; the supply 1 tube 3A and the primary transfer tube 14 A liquid storage container 13 and a g storage container 13 that accommodate the liquid transferred through the primary transfer tube 14 from the transfer side valve 15 provided in between and the collecting section A through the inflow port 13A.
It consists of a discharge tune 16J6 and a volume valve 17 for discharging to the outside the sample liquid transport tank air and the washing liquid for washing supplied from the primary transfer tube 14 to the discharge port 13B of the pipe. Section C includes a secondary transfer tube 22j6 and a secondary transfer on/off valve 36 connected to the sample supply port 13C provided at the bottom of the liquid storage container 13, and a cross tube connection section 21.
The sample liquid in the liquid reservoir 13 is aspirated through the injection liquid reservoir tube 1.
A syringe 20 having a syringe cap 20A for injecting sample liquid into the measuring section (analyzer) 33 through a cross tube connection 21, an injection tube 19 and an injection valve 23, and a waste liquid tank 25 via a waste liquid valve 24. It consists of a waste liquid tube 26 for discharging waste liquid. The syringe 20 is also equipped with a suction end detection switch 27, a primary injection detection switch 28, a quantitative injection stop switch 29, and an injection end detection switch 30 for detecting the position of the syringe rod 20B. The present invention will be described in detail by Shiba and Sei, mentioned above. Collection part A
At this time, the air on-off valve 8 and the transfer side valve 15 are kept closed, the large mouth valve 4 is opened, and the sampling syringe rod 5B of the sampling syringe 5 is suctioned to aspirate the blood collection tube 2, quantitative sampling tube 3, and residual liquid. The pressure inside the fluid path of the tube 6 is reduced, and based on the pressure difference with the biological blood vessel 1, blood (specimen fluid) is smoothly supplied to the quantitative sampling tube 3 via the blood collection tube 2. In addition, the old residual liquid remaining in the blood collection tube 2 is passed through the quantitative sampling tube 3.

[, while being accommodated in the residual liquid suction tube 6, a new sample liquid is subsequently accommodated in the quantitative sampling tube 3 following this residual liquid. Note that at the same time that the residual liquid in the blood collection tube z is suctioned and removed, a small amount of the new sample liquid flowing in is removed while washing away the residual liquid adhering to the inner wall traps of the blood collection tube 2 and the quantitative sampling tube 3. It is accommodated in the liquid suction tube 6 together with the remaining liquid. Therefore, the quantitative sampling tube 3 contains fresh, uncontaminated sample liquid. In addition, when the air opening/closing valve 8, the transfer side valve 15, and the inlet valve 4 are all held closed, the zangling rod 5
If the suction operation by B is performed and the large mouth valve 4 is controlled to be opened after a predetermined period of time has elapsed, the degree of pressure reduction will be high.
It is possible to prevent the risk of air backflowing into the living blood vessel 1 through the blood collection tube 2. The sampling syringe rod 5Bld stops after a predetermined amount of sample liquid has been collected by the sampling end detection switch 31, closes the large mouth valve 4, and closes the air on/off valve 8 and the transfer side valve 15.
and discharge valve 17 are opened. In this state, the air pump 11 is operated and the sample liquid in the quantitative sampling tube 3 is pumped by the air pressure sent out.
The liquid is pneumatically transported to the liquid storage container 13 through the primary transfer tube 14 of the L storage container section B. The transfer time of the sample liquid by this pneumatic transportation is shortened, and the measurement cycle L shown in FIG. 2 is significantly shortened. The sample liquid flows into the liquid reservoir 13 from the inlet 13A and is stored at the bottom. On the other hand, excess air filling the inside of the liquid storage container 13 is discharged to the outside through the primary discharge tube 16 from the discharge port 13B. In the suction/injection part C, the injection valve 23 and the waste liquid valve 24
is held closed, the transfer on-off valve 36 is opened, and the syringe 20
By the suction operation of the syringe rod 20B, the secondary transfer tube 22 and the liquid path of the injection liquid reservoir tube 18 are brought into a reduced pressure state from the inside of the liquid reservoir container 13. Based on this pressure difference,
The sample liquid stored in the bottom of the liquid storage container 13 is transferred to the sample supply port 1.
From 3C, via the secondary transfer tube 22, the injection liquid reservoir tube 1
8 and contain it. Syringe rod zOB&1: When a sufficient amount of sample liquid is stored in the injection liquid reservoir tube 18, the suction end detection switch 27 is activated to stop the rod, the transfer opening/closing valve 36 is closed, and the injection valve z3i is opened. Next, when the transfer on-off valve 36 and the waste liquid valve 24 are kept closed and the injection valve 23 is kept open, the syringe rod 20B
Due to the injection operation, the air layer inside the syringe 20 becomes under high pressure, and the pressure of this air layer causes the injection tube 19 to open and transfer the sample liquid contained in the liquid reservoir (1s) to the analyzer 33. Inject. During this injection operation, the air (bubbles) remaining in the injection tube 19 is pushed out to the analyzer 33 by the sample liquid to be injected, and the injection tube 19 is filled with the sample liquid. At the same time, the analyzer 33 performs a cleaning operation to clean and discharge air bubbles generated inside the analyzer 33 by using the injected air. Air bubbles that have a negative impact on
- Be done. The syringe rod 20B continues to perform the injection operation, and the injection tube 19
, into the analyzer 33, and then press the quantitative injection stop switch 29.
The injection operation is stopped by the operation of . Note that the syringe rod 20B is connected between the position of the suction end detection switch 27 and the position of the primary injection detection switch 28.
The position of the primary injection detection switch 28 should be set so that the internal volume of the syringe 20 when it moves is larger than the internal volume of the injection tube 190. Further, the fixed amount injection stop switch 290 position 1 is set according to the position of the primary injection detection switch 28 according to the internal volume of the predetermined amount of sample liquid to be analyzed. Note that the position of the injection end detection switch 30 is FI4. In this case, the tip of syringe rod 20B is connected to syringe 1.
-'] 20A, and even when the syringe rod 20B stops injecting the tube, a small amount of precipitate remains in the injection tube 19, and the injection tube 1
9 to prevent the risk of air bubbles flowing into the analyzer 33. Furthermore, during the injection operation (・jl, the transfer on-off valve 36 is closed, the large mouth valve 4 is held in the closed state, and the air on-off valve 8, the transfer side valve 15J6, and the discharge valve 17 are opened (2), the sampling The syringe rod 5B removes the residual liquid in the residual liquid suction tube 6.
Discharge into quantitative sampling tube 3. At this point, the residual liquid discharge detection switch 32 stops the operation of the sampling syringe rod 5B after the discharge is completed. When the sampling syringe rod 5B stops, the cleaning liquid pump 9 and the air bong 1 are activated, and the cleaning liquid and air pass through the secondary air tube 7 and remove the residual liquid in the primary transfer tube 14. The inflow I] I 3 A is filled into the teaching chamber 13 for cleaning, and the surplus liquid and air are discharged from the outlet 13B through the discharge tube 16 to the external waste liquid tank 25.
reactor. After the preset cleaning time, the cleaning liquid pump 9 stops, but the air pump 11 continues to supply air and
1. The liquid inside the tank and the liquid storage container I3 are cleaned, and the cleaning liquid is discharged and cleaned 1-1. In this way, when the suction/injection part C is injected/analyzed, the liquid collection part Aj6 and the liquid reservoir part 13 are cleaned.
6111 It is possible to shorten the constant cycle time. After the analysis is completed, the transfer on-off valve 36 is closed, and the injection valve 23,
The waste liquid valve 24 is opened, and the syringe rod 2B performs the injection operation again at t2, and the remaining liquid in the injection liquid reservoir tube 18, the cross tube connection part 2 and the waste liquid A'fS of the injection tube 10 is drained into the waste liquid tube. 26: Discharge to the waste liquid tank 25 and the separation tank 33 through the r6 and the injection pipe 19. This injection operation is stopped by the operation of the injection end detection switch 30. Along with this stop, the transfer on/off valve 36, the injection valve 23, and the waste liquid valve 24 are opened, and the discharge valve 17 is closed, so that the cleaning liquid remaining in the liquid storage container 13 is discharged from the sample supply port 13C by air pressure. via secondary transfer tube 22 and injection tube 19 to analyzer 33 or waste tube 26
The respective liquids are washed and discharged to a waste liquid tank 26 through the process. In this way, the control means (
(not shown) is installed to clean the fluid path after the sample fluid is collected, transferred, and analyzed.
Continuous transition to the next blood collection, transfer, and analysis is possible. In addition, in the quantitative sampling tube 3, the amount of sample liquid to be collected is selected from the amount of sample liquid required for analysis and the amount of residual liquid remaining as a result of stirring in the liquid path of the entire device,
The internal volume of the quantitative sampling tube 3 is set to be the same as this sampling amount. With this configuration,
Since a constant sampling amount is always reproduced without being affected by the accuracy of the Zannolink syringe 5, a high-performance Zannolink syringe 5 is not required. 2. Residual liquid suction port 3B and supply port 3A on both sides of quantitative sampling tube 30
includes secondary air tube 7 left and primary transfer tube 14
Or each connected, ya! -! 7-7 The JL is formed in a straight line with the Noring tube 3, and the sample liquid is transferred smoothly by air pressure. At the awn, the connection part J between the residual liquid suction tube 6 and the residual liquid suction port 3B (and the connection part between the blood collection tube 2 and the supply port 3A is connected to the quantitative sampling naive 3 and the secondary air tube 7 J6 and 1 Next, the liquid passage formed by the transfer tube 14 should be connected in a straight line and at a predetermined angle.Therefore, it is possible to transfer the sample liquid in the printer tube 3 of a fixed length by air pressure. It is possible to avoid the liquid in the residual liquid suction tube 6 and the blood collection tube 2 from being entangled by air pressure and transferred, reducing contamination of the transferred sample liquid.In addition, the cleaning liquid pump 9 After the air pump 11 has stopped, when cleaning the air with the air pump 11, the air pump 11 connects the secondary air tube 7 with The sent air entrains and transfers the remaining cleaning liquid in the cleaning liquid tube 10, and the cleaning time for cleaning the remaining liquid due to the wall air throughout the liquid path becomes long (there is a risk that the cleaning will become insufficient as the air grows thicker). , the secondary air tube 7 and the cleaning liquid naublO
A check valve 34 is provided between the cleaning liquid tube 10 and the cleaning liquid tube 10 to prevent mixing of the cleaning liquid in the cleaning liquid tube 10, which occurs when the sample liquid is transferred by air. Further, after cleaning the liquid path with the cleaning liquid, the cleaning liquid can be easily discharged with air, and the configuration thereof is simplified. The cross tube connection part 21 of the suction/injection part C linearly connects the secondary transfer tube 22 and the waste liquid tube 26, and uses the air pressure of the air pump 11 to transfer the cleaning liquid in the liquid storage container 13 to the sample supply port 13Cj6 and 7. When discharging the liquid from the waste tube 26 to the outside through the secondary transfer tube 22, the flow is linear and has little resistance.7. 【stomach. In particular, when handling a very small amount of sample liquid of 100 μl or less, the inner diameter of the secondary transfer tube 22, waste liquid tube 26, etc. must be particularly thin, 1 mm or less. Also in this case, since the flow resistance is small, the low pressure air pump 11 is sufficient for the flow of the cleaning liquid. Similarly,
The injection liquid reservoir pipe 18 and the injection naube 19 are connected in an IA line through a cross tube connection part 21, and the syringe rod 20
There is little resistance to the injection flow of the sample liquid (injection is easy) that occurs during the injection operation of B. In other words, for example, the blood collection tube 2 made of a silicone tube is connected to the biological blood vessel 1, and the control means is Approximately 90 μl of sample liquid is automatically collected at predetermined time intervals set on the timer of
After that, it is injected into the analyzer 33, where it performs a predetermined analysis, and the results are displayed digitally and output as an analog hold value. In addition, the time of LU1 cycle shown in Fig. 2 can be adjusted arbitrarily from the minimum interval of about 3 minutes to the required time.
Normally, the time from blood collection to measurement value output can be a short time of about 3 minutes. Note that this measurement system forming a continuous closed loop can operate not only automatically but also by a predetermined external signal. As explained above, according to the present invention, the liquid collection section consists of a blood collection tube, a quantitative sampling tube, a Zanfling syringe, and a valve gap, and blood is collected in a reduced pressure state in an A-shaped manner, so that air does not enter the living blood vessel. There is no risk of contamination. It is possible to collect blood according to the internal volume of the quantitative sampling tube, and the reproducibility of the amount of blood collected is good, although it is related to the accuracy of the Zamprer syringe, and by selecting the internal volume appropriately, it is possible to collect a very small amount of blood. It is. 1. The liquid collection container has a liquid collection container, a discharge tube, and a discharge valve, and it is easy to pump the blood sample with air, transfer it with cleaning liquid, and wash it after each press-in. The measurement cycle time can be shortened. °In addition,
The suction/injection part combines the injection liquid reservoir pipe connected to the liquid reservoir container, the syringe, and the valve, and uses switches to detect the operating position of the syringe rod during the syringe operation process, and to set the specified pause time. Even if the amount of liquid to be sampled changes slightly, and even if the liquid path has a small inner diameter for a small amount of sample liquid, it is possible to ensure the reproducibility of a uniform one-time injection amount to the analyzer. Cleaning after cleaning is also reliable. Furthermore, the tube connection part of the liquid collection part and the cross tube connection part of the I-monbetsu/injection part are 7-way. The iL liquid route? Since it is connected in a straight line, 0. Air pumps 2 and 6 with reduced flow resistance and small capacity. Pneumatic transport by a purifying liquid pump, liquid cleaning 2 and cleaning liquid discharge by air are possible, and the form is realized.

[Brief explanation of drawings]

FIG. 1 is a schematic configuration diagram of an embodiment of the present invention, and FIG.
See the operation timing chart in the figure. A: Liquid collection part, B: Liquid reservoir part, C' suction injection part, 2: Blood collection tube, 3: Quantitative sampling tube, 5: +)
- Link syringe, 9: Cleaning liquid pump, 11: Air pump, 13; Liquid storage container, 14: Primary transfer tube, 1
8: Injection liquid reservoir tube, 19: Injection tube, 20: Syringe, 33: Analyzer. Agent: Takeo Yokoya, patent attorney

Claims (1)

[Scope of Claims] (2) A blood collection tube for collecting a sample liquid, a quantitative sampling tube connected to the blood sampling tube, and a predetermined amount of blood connected to the quantitative sampling tube via a residual liquid suction tube to the quantitative sampling tube. a sampling syringe for containing the sample liquid, an air pump connected to the quantitative sampling tube, and a cleaning liquid pump connected to the quantitative sampling tube in parallel with the air pump: the quantitative sampling tube; a primary transfer tube connected to the
a liquid reservoir unit having a liquid reservoir in which the sample liquid contained in the quantitative sampler tube is transferred and stored by the air pump via the primary transfer tube;
a suction/injection unit having a syringe that is connected to the liquid storage container via a secondary transfer tube and aspirates a predetermined amount of the sample liquid in the liquid storage container and injects it into the measurement unit via the injection tube; the syringe; After a predetermined amount of the sample liquid is injected into the measurement unit and the measurement is completed, the cleaning liquid pump sends out the cleaning liquid to each of the sample liquid paths of the liquid collection unit, the liquid storage container R, and the suction/injection unit. A liquid sampling/wheel rolling device characterized by comprising: a control means for continuously repeating the steps of cleaning the cleaning liquid by using the air pump, and discharging the cleaning liquid by sending out air using the air pump. (2) Range of special Ff 3< In the device described in paragraph 1, the air provided between the large mouth valve provided on the liquid sampling tube, the air pump and the cleaning liquid pump, and the quantitative sampling tube. The opening/closing valve and the transfer side valve installed between the quantitative sampling tube and the liquid storage container section are both closed, and the quantitative sampling tube is closed by the suction operation of the limp ring syringe. A liquid sampling and wheel rolling device characterized by comprising a control means for setting a time limit for opening the inlet valve and collecting a predetermined amount of sample liquid after the pressure is reduced. (3) In the device according to claim 1 or 2, after the air and residual liquid remaining in the liquid sampling tube are contained in the residual liquid suction tube by the suction operation of the sampling syringe. . A liquid sampling and wheel rolling device, characterized in that it is equipped with a control means for setting a time limit for subsequently storing the sample liquid into the quantitative sample tube via the liquid sampling tube.