CN114533800B

CN114533800B - Traditional Chinese medicine composition with antibacterial function and method for preparing hand cream by using traditional Chinese medicine composition

Info

Publication number: CN114533800B
Application number: CN202210153821.3A
Authority: CN
Inventors: 刘永琦; 张志明; 魏舒畅
Original assignee: Gansu University of Chinese Medicine
Current assignee: Gansu University of Chinese Medicine
Priority date: 2022-02-19
Filing date: 2022-02-19
Publication date: 2023-06-06
Anticipated expiration: 2042-02-19
Also published as: CN114533800A

Abstract

The invention discloses a traditional Chinese medicine composition with a bacteriostatic function and a method for preparing hand cream by using the same. The traditional Chinese medicine composition and the prepared hand cream can nourish blood, promote tissue regeneration, remove cocoons and moisten skin, and have good inhibition effects on bacteria in the air, escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and enterococcus faecalis.

Description

Traditional Chinese medicine composition with antibacterial function and method for preparing hand cream by using traditional Chinese medicine composition

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a traditional Chinese medicine composition with a bacteriostatic function and a method for preparing hand cream by using the same.

Background

Studies have shown that diethyl ether, 75% ethanol, chlorine-containing disinfectants, peracetic acid, chloroform and other lipid solvents can effectively inactivate 2019-nCoV. However, the prior alcohol, chemical disinfection, hand washing liquid and the like have strong skin corrosiveness and have no pertinence to viruses. The market lacks a targeted disinfectant and a disinfecting hand cleanser, and most of the long-term use of the hand cleanser can damage skin mucous membrane and increase the chance of infection. The traditional Chinese medicine plays a unique role in preventing and treating the new sudden infectious diseases in the past, the national Wei Jian commission, each province and a plurality of traditional Chinese medicine specialists sequentially put forward different traditional Chinese medicine preventing and treating schemes, and the traditional Chinese medicine is proved to play an important role in reducing morbidity, reducing mortality, reducing side effects and improving cure rate.

Disclosure of Invention

The invention aims at providing a traditional Chinese medicine composition with a bacteriostasis function;

the second purpose of the invention is to provide a method for preparing hand cream by using the traditional Chinese medicine composition with the antibacterial function.

The traditional Chinese medicine composition and the hand cream prepared by the traditional Chinese medicine composition can solve the problems of skin injury, allergy, eczema, rhagadia and the like caused by repeated or long-term use of chemical disinfection products.

In order to achieve the above purpose, the invention adopts the following technical scheme:

a traditional Chinese medicine composition with a bacteriostasis function is prepared from the following raw materials in parts by weight: 6-18 parts of rhizoma atractylodis, 6-21 parts of cyrtomium rhizome, 6-21 parts of raw astragalus, 10-30 parts of angelica, 1-12 parts of saffron, 3-15 parts of cortex dictamni, 1-12 parts of lithospermum, 6-18 parts of talcum powder, 1-12 parts of rheum officinale, 6-18 parts of ageratum and 6-21 parts of gallnut.

In order to further realize the invention, the traditional Chinese medicine composition is preferably prepared from the following raw materials in parts by weight: 15 parts of rhizoma atractylodis, 15 parts of cyrtomium rhizome, 15 parts of raw astragalus, 20 parts of angelica, 8 parts of saffron, 8 parts of cortex dictamni, 8 parts of lithospermum, 10 parts of talcum powder, 8 parts of rheum officinale, 10 parts of ageratum and 15 parts of gallnut.

The method for preparing the hand cream by using the traditional Chinese medicine composition with the antibacterial function comprises the following steps of:

(1) Preparing fluid extract:

pulverizing the Chinese medicinal composition, sieving, reflux extracting with 8 times of ethanol solution for 2 times, each for 1-2 hr, filtering, concentrating under reduced pressure at 55-65deg.C until no ethanol smell exists, and obtaining fluid extract;

(2) And (3) preparing a mixed aqueous phase:

uniformly mixing 10-50 parts of the fluid extract obtained in the step (1) with 3-25 parts of propylene glycol, 0.2-2 parts of triethanolamine and 2-15 parts of carbomer to obtain a mixed water phase;

(3) Preparing an oil phase:

heating and melting 0.5-5 parts of simethicone, 2-20 parts of stearyl alcohol, 1-15 parts of polyethylene glycol stearate and 1-15 parts of glyceryl monostearate to prepare an oil phase;

(4) Emulsifying and preparing paste:

adding the mixed water phase obtained in the step (2) and the oil phase obtained in the step (3) into a homogenizer, homogenizing and emulsifying until the color, uniformity, viscosity and oil-water separation meet the requirements, discharging, and packaging.

In order to further realize the invention, the emulsification temperature in the step (4) is 60-80 ℃, and after the emulsification is rapidly stirred for 5-30min at the rotating speed of 3000RPM, the emulsification is slowly stirred for 5-20min at the rotating speed of 30 RPM.

In order to further realize the invention, the pore diameter of the screen mesh of the crushing and sieving in the step (1) is not more than 0.5cm.

In order to further realize the invention, the filtering screen for filtering the extracting solution in the step (1) is 180-220 meshes.

In order to further carry out the present invention, the concentration of the ethanol solution in step (1) is 65 to 76%.

In order to further carry out the present invention, the relative density of the fluid extract in step (1) is 1.01.

The formula principle of the invention:

the recipe is mainly used for eliminating dampness, avoiding dirt, nourishing blood, promoting tissue regeneration, eliminating cocoons and moisturizing skin; monarch drug: rhizoma Atractylodis, invigorating spleen, eliminating dampness, resolving stagnation, removing dirt, promoting radix astragali, invigorating qi, consolidating superficial resistance, removing toxic substances, and promoting granulation; ministerial drugs: cyrtomium fortunei has the effects of killing parasites, clearing heat and detoxicating, saffron has the effects of clearing heat, detoxifying, promoting diuresis, preventing malaria, removing blood stasis, chinese angelica has the effects of replenishing blood, harmonizing blood, moistening dryness, chinese gall has the effects of astringing lung and reducing pathogenic fire, and has the effects of astringing dampness and healing sore; adjuvant drug: cortex Dictamni Radicis has effects of clearing heat and eliminating dampness, dispelling pathogenic wind and removing toxic substances, radix Arnebiae has effects of cooling blood, promoting blood circulation, clearing heat and removing toxic substances, radix et rhizoma Rhei has effects of purging pathogenic heat and toxic substances, removing food stagnation, promoting blood stasis, herba Agastaches has effects of removing turbid pathogen, regulating middle energizer and relieving vomiting, relieving exterior syndrome and relieving summer heat, and pulvis Talci has effects of clearing heat and removing dampness.

The cyrtomium fortunei, the safflower, the angelica and the gallnut are used as ministerial drugs, the cyrtomium fortunei, the bitter and cool, the good effect is killing insects, clearing heat and detoxicating, the principal meaning of materia medica: therefore, it can stop bleeding due to pathogenic heat, so it is bitter in all actions of drying dampness and dispelling cold. However, because of its strong nature, it can dispel the toxic heat. Modern pharmacological studies suggest that cyrtomium fortunei has antiviral and dermatophyte inhibition effects. Dang Gui, ganjing, warm, good at replenishing blood, harmonizing blood and moistening dryness, ming Yi Bie Lu (miscellaneous records of famous physicians): the "warm-stopping pain, removing blood stasis, apoplexy, spasm, sweat, damp arthralgia, middle-jiao and malignant passenger qi, deficiency cold, replenishing five viscera and promoting muscle production". Modern pharmacological researches prove that angelica has antibacterial effects on paratyphoid, escherichia coli, shigella dysenteriae, typhoid and the like in vitro, and can accelerate the healing of the wound surface of rabbit ears by external application, promote local congestion, leukocyte infiltration and fiber infiltration, regenerate new epithelium and the like. Safflower (Tibetan), bitter, pungent and cool, has the effects of clearing heat, detoxifying, promoting diuresis, checking malaria and removing blood stasis, and modern pharmacological researches prove that the alcohol extract and the water extract of safflower have anti-inflammatory effects. The Chinese gall has sour and salty taste, can astringe lung to stop bleeding, resolve phlegm, quench thirst and sweat, has cold qi and can dispel toxic sore and swelling, and in-vitro researches show that the Chinese gall has obvious antibacterial or bactericidal effects on streptococcus, staphylococcus aureus, pseudomonas aeruginosa and the like; the Galla chinensis decoction has inhibiting effect on influenza A PR8 strain virus inoculated to chick embryo. Rhizoma Osmundae, flos Carthami, radix Angelicae sinensis, and Galla chinensis are used as ministerial drugs to assist principal drug such as rhizoma Atractylodis and radix astragali, and has effects in eliminating dampness, invigorating qi, consolidating superficial resistance, removing toxic substances, nourishing blood, promoting granulation, and removing cocoon to moisten skin.

In the recipe, cortex dictamni, lithospermum, rheum officinale, ageratum and talcum powder are taken as adjuvant drugs, and the cortex dictamni, bitter and cold are good at clearing heat and drying dampness, dispelling wind and detoxifying, and the original of materia medica: cortex Dictamni Radicis can be used for treating all scabies, aversion to wind, scabies, waxberry, and skin sore. Modern pharmacological studies have proved that cortex Dictamni Radicis has inhibitory effect on various pathogenic fungi such as Trichophyton. Radix Arnebiae, bitter and cold, has the effects of cooling blood, promoting blood circulation, clearing heat and removing toxic substances, and is described in the theory of medicine: it can be used for treating malignant boil and tinea. "compendium of materia Medica: it is indicated for macula and acne. Modern pharmacological studies prove that lithospermum has an inhibiting effect on staphylococcus aureus and also has an antiviral effect. Talcum powder, sweet and bland, cold. Proved by modern pharmacological researches, the talcum powder has an inhibiting effect on typhoid bacillus and paratyphoid bacillus. Rhubarb, radix et rhizoma Rhei, bitter in flavor and cold in nature, has the actions of purging heat toxin, removing food stagnation and removing blood stasis, and is used in Japanese Su Cao (Japanese Su Cao): and applied to all sores, furuncles and carbuncles. Modern pharmacological researches have proved that the rhubarb has strong antibacterial effect and broad antibacterial spectrum, and has antibacterial effect on typhoid bacillus, dysentery bacillus, vibrio cholerae, dermatophytes and the like. Huo Xiangxing and Gui Jing Xin are slightly warm. Enter spleen, stomach and lung meridians. Has the functions of treating and eliminating turbid pathogen, regulating middle energizer and vomiting, and relieving summer-heat. It is used for treating damp obstruction in middle energizer, gastric fullness and vomiting, summer-heat and dampness exterior syndrome, and modern pharmacological researches prove that wrinkled giant hyssop has the function of sterilizing, and one leaf of the wrinkled giant hyssop can remove halitosis after being orally taken and can be used as a preservative. Talcum powder has effects of clearing heat and eliminating dampness. The cortex dictamni, the lithospermum, the rheum officinale, the ageratum and the talcum powder are taken as adjuvant drugs and are matched with monarch drugs, minister drugs and assistant drugs to treat the combined symptoms and blend the drugs.

The medicines are mutually matched, and have the effects of nourishing blood, promoting tissue regeneration, removing cocoons and moisturizing skin, and can be used for bacteriostasis and disinfection.

Compared with the prior art, the invention has the beneficial effects that:

the traditional Chinese medicine composition and the prepared hand cream can nourish blood, promote tissue regeneration, remove cocoons and moisten skin, and have good inhibition effects on bacteria in the air, escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and enterococcus faecalis.

The invention not only has better skin disinfection effect, but also can protect skin, promote the repair and regeneration of damaged skin, and has no skin irritation and allergy.

Drawings

FIG. 1 is a graph showing the effect of the fluid extract of the Chinese medicinal composition with different concentrations on inhibiting bacteria in the air;

FIG. 2 is a graph showing the effect of the fluid extract of the Chinese medicinal composition with different concentrations on inhibiting Escherichia coli;

FIG. 3 is a graph showing the inhibitory effect of the fluid extract of the Chinese medicinal composition with different concentrations on Staphylococcus aureus;

FIG. 4 is a graph showing the effect of fluid extract of Chinese medicinal composition with different concentrations on inhibiting Pseudomonas aeruginosa;

fig. 5 is a graph showing the inhibitory effect of the fluid extract of the Chinese medicinal composition with different concentrations on enterococcus faecalis.

Detailed Description

The invention is further described below with reference to the drawings and the detailed description.

Examples section:

traditional Chinese medicine composition example 1: 15g of rhizoma atractylodis, 15g of cyrtomium rhizome, 15g of raw astragalus, 20g of angelica, 8g of saffron, 8g of cortex dictamni, 8g of lithospermum, 10g of talcum powder, 8g of rheum officinale, 10g of ageratum and 15g of gallnut.

Traditional Chinese medicine composition example 2: 6g of rhizoma atractylodis, 6g of cyrtomium rhizome, 6g of raw astragalus, 10g of angelica, 1g of saffron, 3g of cortex dictamni, 1g of lithospermum, 6g of talcum powder, 1g of rheum officinale, 6g of ageratum and 6g of gallnut.

Traditional Chinese medicine composition example 3: 18g of rhizoma atractylodis, 21g of cyrtomium rhizome, 21g of raw astragalus, 30g of angelica, 12g of saffron, 15g of cortex dictamni, 12g of lithospermum, 18g of talcum powder, 12g of rheum officinale, 18g of ageratum and 21g of gallnut.

Preparation method of hand cream with antibacterial function example 1:

a method for preparing hand cream by using a traditional Chinese medicine composition with antibacterial function disclosed in a traditional Chinese medicine composition example 1, comprising the following steps:

(1) Preparing fluid extract:

pulverizing the Chinese medicinal composition, sieving with sieve with aperture not larger than 0.5cm, reflux extracting with 70% ethanol solution 8 times the weight of the Chinese medicinal composition for 2 times, each time for 1 hr, filtering the extractive solution (200 mesh), concentrating under reduced pressure at 60deg.C until no ethanol smell exists, to obtain fluid extract with relative density of 1.01;

(2) And (3) preparing a mixed aqueous phase:

uniformly mixing 30ml of the fluid extract obtained in the step (1) with 18ml of propylene glycol, 1ml of triethanolamine and 8ml of carbomer to obtain a mixed water phase;

(3) Preparing an oil phase:

3ml simethicone, 12g stearyl alcohol, 8g polyethylene glycol stearate and 8g glyceryl monostearate are heated and melted to prepare an oil phase;

(4) Emulsifying and preparing paste:

adding the mixed water phase obtained in the step (2) and the oil phase obtained in the step (3) into a homogenizer, rapidly stirring for 20min at a rotating speed of 3000RPM, slowly stirring for 15min at a rotating speed of 30RPM, homogenizing, emulsifying until the color, uniformity, viscosity and oil-water separation meet the requirements, discharging, and packaging.

Preparation method of hand cream with antibacterial function example 2:

a method for preparing hand cream by using a traditional Chinese medicine composition with antibacterial function disclosed in a traditional Chinese medicine composition example 2, comprising the following steps:

(1) Preparing fluid extract:

pulverizing the Chinese medicinal composition, sieving with sieve with aperture not larger than 0.5cm, reflux extracting with 65% ethanol solution 8 times the weight of the Chinese medicinal composition for 2 times, each time for 1 hr, filtering the extractive solution (180 mesh), concentrating under reduced pressure at 55deg.C until no ethanol smell exists, to obtain fluid extract with relative density of 1.01;

(2) And (3) preparing a mixed aqueous phase:

uniformly mixing 10ml of the fluid extract obtained in the step (1) with 3ml of propylene glycol, 0.2ml of triethanolamine and 2ml of carbomer to obtain a mixed water phase;

(3) Preparing an oil phase:

heating and melting 0.5ml simethicone, 2g stearyl alcohol, 1g polyethylene glycol stearate and 1g glyceryl monostearate to obtain an oil phase;

(4) Emulsifying and preparing paste:

adding the mixed water phase obtained in the step (2) and the oil phase obtained in the step (3) into a homogenizer, rapidly stirring for 5min at a rotating speed of 3000RPM, slowly stirring for 5min at a rotating speed of 30RPM, homogenizing, emulsifying until the color, uniformity, viscosity and oil-water separation meet the requirements, discharging, and packaging.

Preparation method of hand cream with antibacterial function example 3:

a method for preparing hand cream by using a traditional Chinese medicine composition with antibacterial function disclosed in a traditional Chinese medicine composition example 3, comprising the following steps:

(1) Preparing fluid extract:

pulverizing the Chinese medicinal composition, sieving with sieve with aperture not larger than 0.5cm, reflux extracting with 76% ethanol solution 8 times the weight of the Chinese medicinal composition for 2 times, each time for 2 hr, filtering the extractive solution (220 mesh), concentrating under reduced pressure at 65deg.C until no ethanol smell exists, and obtaining fluid extract with relative density of 1.01;

(2) And (3) preparing a mixed aqueous phase:

uniformly mixing 50ml of the fluid extract obtained in the step (1) with 25ml of propylene glycol, 2ml of triethanolamine and 15ml of carbomer to obtain a mixed water phase;

(3) Preparing an oil phase:

5ml of simethicone, 20g of stearyl alcohol, 15g of polyethylene glycol stearate and 15g of glyceryl monostearate are heated and melted to prepare an oil phase;

(4) Emulsifying and preparing paste:

adding the mixed water phase obtained in the step (2) and the oil phase obtained in the step (3) into a homogenizer, rapidly stirring for 30min at a rotating speed of 3000RPM, slowly stirring for 20min at a rotating speed of 30RPM, homogenizing, emulsifying until the color, uniformity, viscosity and oil-water separation meet the requirements, discharging, and packaging.

Experimental example part:

materials and methods:

1. experimental strains, see table 1:

。

2. experimental drugs:

the experimental medicine adopts the preparation method of the hand cream with the antibacterial function, and the fluid extract obtained in the example 1 is used for antibacterial experiments, and the concentration of the liquid medicine is 20mg/100ml.

3. Experimental materials:

agarose basal medium (Qingdao sea Bo Biotechnology Co., ltd.), sterile cotton swab (Zhejiang European technology Co., ltd.), physiological saline (Sichuan Korea pharmaceutical Co., ltd.).

4. Experimental instrument:

biological safety cabinet (manufacturer: shandong Boke biotechnology Co., ltd.; model: BSC-1100IIB 2), biochemical incubator (manufacturer: tianjin Tes Co., ltd.; model: SXP-70B); autoclave (manufacturer: shanghai Shen An; model: LDZX-50 KBS).

5. Preparation of bacterial suspension:

inoculating the preserved strain into solid culture medium, and culturing at 37deg.C for 16-24 hr. Selecting single colony, placing into normal saline, shaking thoroughly, mixing, performing turbidimetry, correcting bacterial concentration to 0.2 Mc10 standard, equivalent to 4 ×10 ⁷ CFU/ml。

6. Preparing a medicine-containing culture medium:

in a biosafety cabinet, adding 10ml of medicine liquid and liquid culture medium into a culture dish respectively, wherein the amounts of the medicine liquid and the culture medium added are 2ml,1.2ml and 0.6ml respectively: 8ml,8.8ml and 9.4ml, the ratio of the prepared liquid medicine to the culture medium is 1:4, 1:8 and 1:16 respectively, and the high dose, the medium dose and the low dose are respectively carried out, and each concentration is repeated three times.

7. Bacterial inoculation and cultivation:

sterilizing the mixed agar culture media containing medicines with different concentrations by high-pressure steam, keeping the temperature at 45 ℃, pouring the plates at 10ml each time, dipping the plates with sterile cotton swabs after cooling, rotationally squeezing out redundant bacterial liquid on the inner wall of a test tube, inoculating, uniformly coating the bacterial liquid on the whole culture medium by the sterile cotton swabs, repeating for 3 times, rotating the plates for 60 degrees each time, and finally winding 2 circles around the plates, and uniformly coating. The inoculated dishes were placed in a 37℃incubator, and the results were observed after culturing for 24 hours.

Experimental example 1: inhibition of bacterial in air by fluid extract of Chinese medicinal composition with different concentrations

The fluid extract of the Chinese medicinal composition with high, medium and low concentrations can inhibit the growth of bacteria in the air, no bacterial lawn or bacterial colony growth is seen on the culture medium, and the control group has bacterial colony growth. See table 2, fig. 1, wherein a is a control group, and B is a group to which the fluid extract of the Chinese medicinal composition is added.

。

Experimental example 2: inhibiting effect of Chinese medicinal composition fluid extract with different concentrations on Escherichia coli

The high-concentration Chinese medicinal composition fluid extract can obviously inhibit the growth of escherichia coli, and no bacteria grow on a culture medium. The fluid extract of the Chinese medicinal composition in the medium and low dosage groups has no inhibition effect on Escherichia coli, and the bacteria can grow in a lawn shape, and the lower the medicinal concentration is, the thicker the lawn is, as shown in Table 3 and figure 2.

。

Experimental example 3: inhibition of staphylococcus aureus by different concentrations of Chinese medicinal composition fluid extract

The fluid extracts of the Chinese medicinal compositions in the high, medium and low dosage groups all show the inhibition effect on staphylococcus aureus, no lawn is formed, the colony growth phenomenon is visible, and the higher the concentration is, the smaller the number of the colonies is, and the smaller the colonies are. Colony counts were statistically significant (P < 0.01) in the high and medium concentration groups compared to the low concentration groups, and statistically significant (P < 0.01) in the high concentration groups compared to the medium concentration groups, as shown in table 4, fig. 3.

。

Experimental example 4: inhibition effect of Chinese medicinal composition with different concentrations on pseudomonas aeruginosa

The fluid extract of the Chinese medicinal composition in the high, medium and low dosage groups has no inhibition effect on pseudomonas aeruginosa, and the bacteria are seen to be in a lawn-like growth phenomenon, have a copper green color, and the thicker and denser the growing lawn is the lower the medicament concentration is, as shown in table 5 and fig. 4.

。

Experimental example 5: inhibition effect of Chinese medicinal composition fluid extract with different concentrations on enterococcus faecalis

The fluid extract of the Chinese medicinal composition in the high, medium and low dosage groups has good effect of inhibiting enterococcus faecalis, and the bacterial growth is not seen in the three medicament concentrations, as shown in Table 6 and figure 5.

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Claims

1. The traditional Chinese medicine composition with the antibacterial function is characterized by being prepared from the following raw materials in parts by weight: 6-18 parts of rhizoma atractylodis, 6-21 parts of cyrtomium rhizome, 6-21 parts of raw astragalus, 10-30 parts of angelica, 1-12 parts of saffron, 3-15 parts of cortex dictamni, 1-12 parts of lithospermum, 6-18 parts of talcum powder, 1-12 parts of rheum officinale, 6-18 parts of ageratum and 6-21 parts of gallnut.

2. The traditional Chinese medicine composition with the antibacterial function as claimed in claim 1, which is characterized by being prepared from the following raw materials in parts by weight: 15 parts of rhizoma atractylodis, 15 parts of cyrtomium rhizome, 15 parts of raw astragalus, 20 parts of angelica, 8 parts of saffron, 8 parts of cortex dictamni, 8 parts of lithospermum, 10 parts of talcum powder, 8 parts of rheum officinale, 10 parts of ageratum and 15 parts of gallnut.

3. The method for preparing the hand cream by using the traditional Chinese medicine composition with the antibacterial function as claimed in any one of claims 1 to 2, which is characterized by comprising the following steps:

(1) Preparing fluid extract:

(2) And (3) preparing a mixed aqueous phase:

(3) Preparing an oil phase:

(4) Emulsifying and preparing paste:

4. A method for preparing a hand cream with antibacterial function as claimed in claim 3, characterized in that: and (3) in the step (4), the emulsification temperature is 60-80 ℃, the stirring is fast carried out for 5-30min at the rotating speed of 3000RPM, and then the stirring is slowly carried out for 5-20min at the rotating speed of 30 RPM.

5. A method for preparing a hand cream with antibacterial function as claimed in claim 3, characterized in that: the mesh size of the crushing and sieving screen in the step (1) is not more than 0.5cm.

6. A method for preparing a hand cream with antibacterial function as claimed in claim 3, characterized in that: the filtering screen for filtering the extracting solution in the step (1) is 180-220 meshes.

7. A method for preparing a hand cream with antibacterial function as claimed in claim 3, characterized in that: the concentration of the ethanol solution in the step (1) is 65-76%.

8. A method for preparing a hand cream with antibacterial function as claimed in claim 3, characterized in that: the relative density of the fluid extract in the step (1) is 1.01.