CN114525175B - Bicontinuous phase extracting solution, preparation method thereof and extraction method applied to frankincense - Google Patents
Bicontinuous phase extracting solution, preparation method thereof and extraction method applied to frankincense Download PDFInfo
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- CN114525175B CN114525175B CN202111673261.6A CN202111673261A CN114525175B CN 114525175 B CN114525175 B CN 114525175B CN 202111673261 A CN202111673261 A CN 202111673261A CN 114525175 B CN114525175 B CN 114525175B
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/32—Burseraceae (Frankincense family)
- A61K36/324—Boswellia, e.g. frankincense
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/20—Recycling
Abstract
The application relates to the field of preparation of compounds, in particular to a bicontinuous phase extracting solution, a preparation method thereof and an extracting method applied to frankincense. The bicontinuous phase extracting solution consists of the following components in parts by weight, and 20-25 parts of deionized water; 32-45 parts of polyalcohol; 12-25 parts of polyglycerols; 2-10 parts of alkanes; 2-10 parts of ester grease; 1-5 parts of vegetable oil. The bicontinuous phase extracting solution obtained by the application can effectively retain the effective components in the frankincense besides ensuring the extracting efficiency when being applied to the extraction of the frankincense, and the extracted frankincense contains 3.526 percent (35.26 g/1000 g) of octyl acetate, 28.63 percent (286.3 g/1000 g) of triterpene total organic acid and 16.26 percent (162.6 g/1000 g) of total polysaccharide.
Description
Technical Field
The application relates to the field of medicine extraction and preparation thereof, in particular to a bicontinuous phase extracting solution, a preparation method thereof and an extraction method applied to frankincense.
Background
The olibanum is resin oozed from bark of Boswellia carterii (Boswellia carterii Birdw.) of Boswellia of Oleraceae and Boswellia abalone (Boswelia bhaw-dajiana Birdw.), and is a plant drug with wide development prospect. The research shows that the frankincense extract has pharmacological effects of activating blood, relieving pain, detumescence, promoting granulation, anti-inflammatory, anti-tumor, anti-oxidation, reducing blood sugar and the like, so how to extract the frankincense with high efficiency is also a main research subject at the present stage.
As known, the main active ingredients of olibanum include pentacyclic triterpenes, tetracyclic triterpenes, macrocyclic diterpenes, polysaccharides, disaccharides, monosaccharides, alkenes, esters, alcohols, alkanoic acids and the like, and the ingredients are complex and various, and contain fat-soluble ingredients and water-soluble ingredients at the same time, so that the extraction method is generally complicated, and the efficiency cannot be guaranteed.
The extraction method in the related art is a water decoction method: cutting or pulverizing Olibanum to obtain coarse powder, placing into a proper decocting device, soaking Olibanum in water for proper time, heating to boil, keeping slight boiling for a certain time, separating decoction, decocting residues for several times until the decoction is light in taste, mixing decoctions, and concentrating to obtain Olibanum extract.
Li Pinghua and the like have adopted steam distillation, ultrasonic extraction and Soxhlet extraction methods respectively, and the results show that the content of octyl acetate in volatile oil obtained by steam distillation is higher than that of the volatile oil obtained by other methods, namely 2.92 percent, zheng Yuli and the like are extracted by ethanol and ethyl acetate, and meanwhile, the content of total triterpene organic acid in the olibanum is about 7 percent by combining an acid-soluble alkali precipitation method, and the extraction and application of olibanum polysaccharide are rarely reported.
The extraction method has low extraction efficiency, the components in the extract mainly comprise water-soluble components, a small amount of fat-soluble and volatile oil, the loss of water-insoluble components in the materials is large, impurities are easy to generate, and the purification of the product is not facilitated.
Therefore, some enterprises use a steam distillation-ethanol continuous extraction method to extract the effective components in the olibanum, but the extraction steps are too complicated, the energy consumption is higher, the production cost of the enterprises is greatly increased, and the actual production is not facilitated.
In summary, there is an urgent need to provide an extraction method capable of efficiently and conveniently extracting olibanum.
Disclosure of Invention
The application provides a bicontinuous phase extracting solution, a preparation method thereof and an extraction method applied to frankincense, in order to ensure the extraction efficiency and simplify the extraction process.
In a first aspect, the present application provides a bicontinuous phase extraction solution, which adopts the following technical scheme:
the bicontinuous phase extracting solution is characterized by comprising a phase A, a phase B and a phase C, wherein the phase A comprises the following components in parts by weight:
20-25 parts of deionized water;
32-45 parts of polyalcohol;
1-5 parts of sodium surfactin;
the phase B consists of the following components in parts by weight:
12-25 parts of polyglycerols;
2-10 parts of alkanes;
2-10 parts of ester grease;
the phase C consists of the following components in parts by weight:
1-5 parts of vegetable oil.
By adopting the technical scheme, the bicontinuous phase extracting solution prepared from the water-soluble components, the grease and the surfactant has higher extraction rate when the frankincense is extracted, the effective components are not easy to cause loss, and the obtained frankincense extract contains 3.52 percent of octyl acetate, 28.63 percent of triterpenes total organic acid and 16.28 percent of total polysaccharide.
The analysis is possible that the bicontinuous phase extracting solution simultaneously contains water-soluble components and grease, and the water-soluble components and the grease can form a stable microemulsion system under the action of a surfactant, the microemulsion system is easy to be similar to the components in the olibanum, and the water-soluble components can be wrapped in the inner core of the microemulsion, so that the loss and activity loss phenomena of the microemulsion are effectively reduced, and the extraction rate of the bicontinuous phase extracting solution is further ensured;
the sodium surfactin is added to promote the emulsification effect of the microemulsion as an anionic emulsifier, and can promote the extraction of volatile oil-based liposoluble components, and after being compounded with polyalcohol and grease, the hydrophilic-lipophilic balance value of the bicontinuous phase extract can be effectively regulated, wherein the HLB value of the extract is 3-10.
Preferably, the polyol is one or more of 1, 3-butanediol, dipropylene glycol, 1, 2-propanediol, 1, 2-pentanediol, hexanediol, glycerol, diglycerol, and polyethylene glycol.
By adopting the technical scheme, the polyalcohol of the components can not only effectively reduce the hydrophilicity of the polyglycerol emulsifier, but also be compounded with the sodium surfactin to adjust the HLB value of the bicontinuous phase extract, thereby forming the stable bicontinuous phase extract.
Preferably, the polyglycerin is one or more of polyglyceryl-2 stearate, polyglyceryl-2 isostearate, polyglyceryl-4 stearate, polyglyceryl-4 pentastearate, polyglyceryl-5 trioleate, polyglyceryl-6 laurate, polyglyceryl-6 stearate, polyglyceryl-6 tristearate, polyglyceryl-7 dioleate, polyglyceryl-10 laurate, polyglyceryl-10 isostearate, polyglyceryl-10 diisostearate, polyglyceryl-10 dioleate, polyglyceryl-10 tristearate, polyglyceryl-10 pentastearate, polyglyceryl-10 dicaprate.
By adopting the technical scheme, polyglycerols of the components can be used as an emulsifier, so that the molding of a bicontinuous-phase microemulsion system is effectively promoted, and meanwhile, the polyglycerols can be compounded with sodium surfactin and polyalcohol to reach a proper HLB value, so that a stable bicontinuous-phase extract is formed.
Preferably, the alkane is squalane, hydrogenated polyisobutene, hydrogenated polydecene, C 12-19 Alkane, month hydrogenation C 6-14 One or more of olefin polymers and isododecane.
By adopting the technical scheme, the selected alkane can form a stable bicontinuous phase solution, because the affinity of the grease to water and the influence of the grease on the directional arrangement of the surfactant can influence the preparation of bicontinuous phases, the grease with different molecular weights in the emulsification process has different performances, and the grease with lower molecular weight and viscosity can easily obtain fine nano-emulsified particle size and also easily form bicontinuous phases (such as alkane and essential oil).
Preferably, the ester oil is caprylic/capric triglyceride, ethylhexyl palmitate, isopropyl myristate, dioctyl carbonate, isoamyl laurate, butylene glycol dicaprylate/dicaprate, tri (ethylhexanoate), cetyl ethylhexanoate, and di C 12-13 Alcohol malate, diisostearyl alcohol malate, pentaerythritol tetra (ethylhexanoate), pentaerythritol tetraisostearate, C 12-15 One or more of the benzoates.
By adopting the technical scheme, the nanoemulsion formed by the medium chain triglyceride oil is selected to have a lower curvature radius, the formed nanoemulsion has small particle size, and the curvature radius of different esters is smaller than that of long chain triglyceride along the medium chain triglyceride, so that the rule is also applicable to the formation of bicontinuous phases.
Preferably, the vegetable oil is one or more of jojoba seed oil, shea butter, sweet almond oil, castor oil, grape seed oil, sunflower seed oil, white pool seed oil and linseed oil.
By adopting the technical scheme, the vegetable oil and fat of the components can be effectively compounded with the ester oil and fat and polyglycerols, which is favorable for the formation of a bicontinuous phase, wherein the white pool flower seed oil contains a high-efficiency natural oxidant, is the most stable vegetable oil known in nature, does not contain conjugated double bonds, is not easy to react, and contains 18 percent of docosadienic acid C 22:2 The structure of DHA is very similar to that of the extract, and the extract provides excellent skin feel for the application of the subsequent frankincense extract.
In a second aspect, the application provides a preparation method of a bicontinuous phase extracting solution, which adopts the following technical scheme: a preparation method of a bicontinuous phase extracting solution comprises the following steps:
a1, heating the phase A to 80-85 ℃ respectively, and stirring for 15-30min at 300-500 r/min; heating the phase B to 80-85 ℃, and stirring for 5-10min at 300-500 r/min;
a2, mixing the phase B and the phase A, and stirring for 2-5min at 1000-1200r/min to obtain an AB mixed phase;
and A3, finally cooling the AB mixed phase to 50-60 ℃, adding the C phase, stirring for 5-10min at 300-500r/min, filtering and discarding filter residues to obtain the bicontinuous phase extract.
By adopting the technical scheme, the preparation steps are simpler, the conditions are easy to control, the obtained bicontinuous phase extracting solution has stable and uniform performance, can effectively form a microemulsion system, and has higher extraction rate when being particularly applied to the extraction of the frankincense, thus having wide application prospect.
In a third aspect, the application provides a method for extracting frankincense by using bicontinuous phase extracting solution, which adopts the following technical scheme:
an extraction method of Olibanum extract, which uses any of the above bicontinuous phase extractive solutions, comprises the following steps:
adding Olibanum (at a feed liquid ratio of 1 (10-20)) into bicontinuous phase extractive solution, and extracting at 50-85deg.C for 0.5-3 hr to obtain Olibanum extract.
By adopting the technical scheme, when the obtained bicontinuous phase extracting solution is applied to the extraction of the frankincense, the steps are simpler, the energy consumption is lower, the extraction rate is higher, and the obtained frankincense extract contains 3-4% of octyl acetate, 20-30% of triterpenes total organic acid and 15-20% of total polysaccharide.
In summary, the application has the following beneficial effects:
1. the bicontinuous phase extracting solution is prepared by selectively compounding the polyglycerol emulsifier, the polyalcohol, the alkane grease and the ester grease, has higher extraction rate and less loss of effective components when the frankincense is extracted, and the obtained frankincense extract contains 3.52 percent of octyl acetate, 28.63 percent of triterpene total organic acid and 16.28 percent of total polysaccharide content;
2. according to the preparation method of the bicontinuous phase extracting solution, the frankincense extracting solution with three effective products is directly obtained through a one-step extracting step, and the post-treatment step is not needed;
3. when the bicontinuous phase extracting solution is applied to the extraction of the frankincense, the polyalcohol and the grease are selectively matched through the synergistic effect of the polyglycerol emulsifier and the sodium surfactin, so that the extraction efficiency is remarkably improved, and the extraction of the active ingredients of the frankincense is ensured.
Drawings
FIG. 1 is a gas chromatogram of octyl acetate in the volatile oil of the Olibanum extract obtained in application example 1 of the present application;
FIG. 2 is an ultraviolet spectrum of total triterpene organic acid in Boswellia serrata extract obtained in application example 1 of the present application;
FIG. 3 is an ultraviolet spectrum of total polysaccharides in the Boswellia serrata extract obtained in application example 1 of the present application.
Detailed Description
The application is further illustrated in the following examples and figures 1-3:
the materials and equipment used in the examples and assays of the present application are commercially available except as specifically described below:
the mastic used in the application is Egyptian mastic, the application takes the mastic as a research object, wherein the main component of volatile oil is octyl acetate with the content of 5.6% by gas chromatography test, the content of triterpene total organic acid with the content of 47.8% by ultraviolet spectrophotometer test, and the content of total polysaccharide with the content of 26.5% by ultraviolet spectrophotometer test;
and (2) polymer: polyglycerol-based surfactants such as: polyglycerol-5 trioleate and polyglycerol-10 diisostearate, purchased from sun chemistry, japan;
the detection device UV-2201 type ultraviolet-visible spectrophotometer is purchased from Shimadzu corporation;
the detection device Agilent 6890N FID detector gas chromatograph was purchased from Agilent company, usa.
Examples
Examples 1 to 6
The bicontinuous phase extracting solution comprises the following components in parts by weight as shown in table 1 and is prepared by the following steps: a1, heating the phase A to 80-85 ℃ respectively, and stirring for 20min at 400 r/min; heating the phase B to 80-85 ℃, and stirring for 5min at 400 r/min;
wherein the polyhydric alcohol in the A phase is 1, 3-butanediol;
the polyglycerin in the phase B consists of polyglycerin-5 trioleate and polyglycerin-10 diisostearate according to a weight ratio of 4:1; alkanes are C 12-19 An alkane; the ester oil is triglyceride (ethyl caproic acid);
a2, mixing the phase B and the phase A, and stirring for 3min at 1300r/min to obtain an AB mixed phase;
a3, finally cooling the AB mixed phase to 50-60 ℃, adding the C phase, and stirring for 5min at 400r/min to obtain a bicontinuous phase extract; wherein the vegetable oil in phase C is Beacon seed oil.
Table 1 Components and weights (kg) of examples 1 to 6
Comparative example 1
The bicontinuous phase extract was the same as in example 1 except that it did not contain the sodium surfactin component.
Example 7
The bicontinuous phase extract was the same as in example 1 except that the polyol in phase A was glycerol.
Example 8
A bicontinuous phase extract was the same as in example 1 except that the polyol in phase A was 1, 3-butanediol and dipropylene glycol in a weight ratio of 1:1.
Example 9
The bicontinuous phase extract was the same as in example 1, except that the polyglycerin in phase A was polyglycerin-5 trioleate.
Example 10
A bicontinuous phase extract was the same as in example 1, except that the polyglycerin type A phase was polyglycerin-10 diisostearate.
Example 11
A bicontinuous phase extract was the same as in example 1, except that the alkane in phase A was squalane.
Example 12
Bicontinuous phase extract with alkane as C in phase A 12-19 The procedure of example 1 was followed except that the alkane and isododecane were used in the weight ratio of 1:3.
Example 13
The bicontinuous phase extract was the same as in example 1, except that the ester oil in phase A was ethylhexyl palmitate.
Example 14
The bicontinuous phase extract was the same as in example 1 except that the ester oil in phase A was composed of tri (ethyl hexanoate) glyceride and isopropyl myristate in a weight ratio of 1:2.
Example 15
The bicontinuous phase extract was the same as in example 1, except that the vegetable oil in phase A was sunflower seed oil.
Example 16
The bicontinuous phase extract was the same as in example 1 except that the vegetable oil in phase A was composed of white pool seed oil and sunflower seed oil in a weight ratio of 1:0.5.
Detection test
The content of octyl acetate in the bicontinuous phase mastic extract was analyzed using an Agilent 689ON gas chromatograph, tested using an HP-5 quartz capillary column (30 m x 0.32mm x 0.25 μm), and warmed according to the program; the initial temperature is 80 ℃, kept for 3min, and the temperature is raised to 200 ℃ at the speed of 10 ℃ per minute, and kept for 5min; the temperature of the sample inlet is 250 ℃, and the temperature of the detector is 220 ℃; the flow rate of nitrogen is 4mL/min; the sample was introduced in an amount of 1. Mu.l.
Testing the total triterpene organic acid and total polysaccharide content in the bicontinuous phase Olibanum extractive solution by using UV-2201 type ultraviolet-visible spectrophotometer, wherein the total triterpene organic acid is tested by vanillin-perchloric acid chromogenic method, and the absorbance is tested at 548 nm; the total polysaccharide was measured by phenol-sulfuric acid method and absorbance was measured at 490 nm.
Application example
Application example 1
A Olibanum extract, using the bicontinuous phase extract of example 1, comprises the following steps:
1000g of frankincense is added into the bicontinuous phase extracting solution according to the feed liquid ratio of 1:10, and extraction is carried out for 2 hours at 50 ℃ to obtain the frankincense extract containing 35.2g of octyl acetate, 456.3g of triterpene organic acid and 162.6g of polysaccharide.
Application examples 2 to 8
The difference between the boswellia extract and the application example 1 is that the bicontinuous phase extract is used in different conditions, and the specific correspondence is shown in the table below.
Table: use condition comparison table of bicontinuous phase extracting solution in application examples 2-8
| Group of | Bicontinuous phase extract |
| Application example 2 | From example 2 |
| Application example 3 | From example 3 |
| Application example 4 | From example 4 |
| Application example 5 | From example 5 |
| Application example 6 | From example 6 |
| Application example 7 | From comparative example 1 |
| Application example 8 | From comparative example 2 |
The extract of Boswellia carterii obtained in the above application examples 1 to 8 was extracted, and the contents of the components were measured in units of (g/1000 g of Boswellia carterii) according to the above measurement procedures and measurement standards, and the average value of the measurement results was recorded in the following table.
As can be seen from the above table, the mastic extracts obtained in application examples 1-6 have octyl acetate content of 8.1-35.2 (g/1000 g mastic); the content of triterpene total organic acid is 156.3-286.3 (g/1000 g Olibanum); the total polysaccharide content is 130.1-162.6 (g/1000 g Olibanum);
the bicontinuous phase extracting solution prepared from the water-soluble components, the grease and the surfactant has higher extraction rate when the frankincense is extracted, and the active ingredients in the frankincense are not easy to lose in the extraction process.
As can be seen from the table, the extract of Boswellia carterii obtained in application example 7 has the components of 6.51 (g/1000 g of Boswellia carterii), 264.2 (g/1000 g of Boswellia carterii), and 110.5 (g/1000 g of Boswellia carterii), respectively, which is reduced by 81.5%, 40.1% and 32.4% compared with the extract of Boswellia carterii in application example 1;
the blending of the sodium surfactin can promote the emulsification effect of the microemulsion, can promote the extraction of volatile oil-based liposoluble components, and can effectively adjust the hydrophilic-lipophilic balance value of the bicontinuous phase extract after being compounded with the polyalcohol and the grease, wherein the HLB value is 3-10.
In conclusion, the bicontinuous phase extracting solution prepared from the components and the content can remarkably improve the extraction efficiency of the frankincense, and the analysis is probably because the bicontinuous phase extracting solution is prepared from water-soluble components, grease and a surfactant, and the water-oil can form stable and uniform bicontinuous phases under the action of the surfactant, the components in the bicontinuous phases are similar to each other, and the water-soluble components can be wrapped in the inner core of the micro-emulsion, so that the phenomena of loss and activity loss of the bicontinuous phase extracting solution are effectively reduced, and the extraction rate of the bicontinuous phase extracting solution is ensured.
Application examples 9 to 10
A Olibanum extract is different from application example 1 in that the use condition of the bicontinuous phase extract is different
The correspondence is shown in the table below.
Table: use condition comparison table of bicontinuous phase extracting solution in application examples 9-10
| Group of | Bicontinuous phase extract |
| Application example 9 | From example 7 |
| Application example 10 | From example 8 |
The extract of Boswellia carterii obtained in the above application examples 9 to 10 was extracted, and the contents of the components were measured according to the above measurement procedures and measurement standards, and the average value of the measured results was shown in the following table.
As can be seen from the above table, the extract of Boswellia carterii obtained in application examples 1, 9-10 has a octyl acetate content of 15.3-35.2 (g/1000 g of Boswellia carterii); the content of triterpene total organic acid is 200.1-286.3 (g/1000 g Olibanum); the total polysaccharide content is 150.5-162.6 (g/1000 g Olibanum); it can be seen that the bicontinuous phase extract prepared from the polyols of the above components all extract each active ingredient of mastic gum, wherein application example 1 is a preferred application example.
As can be seen from the above table, the content of each component in application example 9 is reduced to a different degree compared with application example 1, the content of octyl acetate is only 15.3 (g/1000 g of frankincense), and is reduced by 56.5% compared with application example 1; the triterpene total organic acid content is only 200.1 (g/1000 g olibanum), which is reduced by 30.1% compared with application example 1; the polysaccharide content is only 150.5 (g/1000 g of frankincense), which is 7% lower than that of application example 1; the content of each component in application example 10 was not greatly changed from that in application example 1.
In summary, it can be seen that the bicontinuous phase extract prepared by compounding the above-mentioned single 1, 3-butanediol or 1, 3-butanediol and dipropylene glycol can significantly improve the extraction efficiency of mastic, and the reason for this is probably that the addition of 1, 3-butanediol or dipropylene glycol increases the solubility of the oil and the surfactant phase and the intersolubility between the aqueous solution and the surfactant, and that glycerol has an opposite tendency, thereby affecting the extraction of fat-soluble components.
Application examples 11 to 12
The difference between the boswellia extract and the application example 1 is that the bicontinuous phase extract is used in different conditions, and the specific correspondence is shown in the table below.
Table: use condition comparison table of bicontinuous phase extracting solution in application examples 11-12
| Group of | Bicontinuous phase extract |
| Application example 11 | From example 9 |
| Application example 12 | From example 10 |
The extract of Boswellia carterii obtained in the above application examples 11 to 12 was extracted, and the contents of the components were measured according to the above measurement procedures and measurement standards, and the average value of the measurement results was recorded in the following table.
As can be seen from the above table, the extract of Boswellia carterii obtained in application examples 1, 11-12 has octyl acetate content of 25.5-35.2 (g/1000 g of Boswellia carterii); the content of triterpene total organic acid is 180.1-286.3 (g/1000 g Olibanum); the total polysaccharide content is 150.6-162.6 (g/1000 g Olibanum); it can be seen that the bicontinuous phase extract prepared from the polyglycerin of the above components is used for extracting each effective component in Olibanum, wherein application example 1 is a preferred application example.
In summary, it can be seen that the bicontinuous phase extract prepared in the above example 1 can significantly improve the extraction efficiency of mastic gum, and the reason for this analysis may be that the selection of the surfactant has a great influence on the preparation of bicontinuous phase microemulsion, the structure of the hydrophobic group affects the arrangement tendency of the surfactant on the oil-water interface, and further affects the formation of bicontinuous phase, and experiments find that the combination of polyglycerol-5 trioleate and polyglycerol-10 diisostearate can form a relatively stable bicontinuous phase, which is related to the moderate HLB value and the suitable hydrophobic group, and the matching of the HLB value of the suitable surfactant helps to extract the effective components in mastic gum.
Application examples 13 to 14
The difference between the boswellia extract and the application example 1 is that the bicontinuous phase extract is used in different conditions, and the specific correspondence is shown in the table below.
Table: use condition comparison table of bicontinuous phase extracting solution in application examples 13-14
The extract of Boswellia carterii obtained in the above application examples 13 to 14 was extracted, and the contents of the components were measured according to the above measurement procedures and measurement standards, and the average value of the measurement results was recorded in the following table.
As can be seen from the above table, the extract of Boswellia carterii obtained in application examples 1, 13-14 has octyl acetate content of 20.2-35.2 (g/1000 g of Boswellia carterii); the content of triterpene total organic acid is 198.2-286.3 (g/1000 g Olibanum); the content of total polysaccharide is 140.2-162.6 (g/1000 g Olibanum); it can be seen that the bicontinuous phase extract prepared from the alkanes of the above components all extract each active ingredient of Olibanum, wherein application example 1 is a preferred application example.
In summary, the bicontinuous phase extract prepared from the alkane oil can remarkably improve the extraction efficiency of the fat-soluble components in the frankincense, and the analysis is probably due to the fact that alkane oil with lower molecular weight and lower viscosity can easily obtain the fine nano emulsion particle size, so that bicontinuous phases are easily formed, and the extraction rate of the fat-soluble components is promoted, see application examples 13-14 and corresponding data thereof.
Application examples 15 to 16
The difference between the boswellia extract and the application example 1 is that the bicontinuous phase extract is used in different conditions, and the specific correspondence is shown in the table below.
Table: use condition comparison table of bicontinuous phase extract in application examples 15-16
| Group of | Bicontinuous phase extract |
| Application example 15 | From example 13 |
| Application example 16 | From example 14 |
The respective component contents of the extract of Boswellia carterii obtained in the above application examples 15 to 16 were measured according to the above measurement procedures and measurement standards, and the average value of the measured results was recorded in the following table.
As can be seen from the above table, the extract of Boswellia carterii obtained in application examples 1, 15-16 has a octyl acetate content of 28.6-35.2 (g/1000 g of Boswellia carterii); the content of triterpene total organic acid is 246.5-286.3 (g/1000 g Olibanum); the total polysaccharide content is 130.6-162.6 (g/1000 g Olibanum); it can be seen that the bicontinuous phase extract prepared from the esters of the above components all extract each active ingredient of Olibanum, wherein application example 1 is a preferred application example.
In conclusion, the selection of the ester components can influence the content of each effective substance in the bicontinuous phase extracting solution, wherein the selection of the medium-chain triglyceride oil is beneficial to the formation of bicontinuous phases, so that the extraction of each effective component in the frankincense is improved, and the extraction rate of the bicontinuous phase extracting solution is ensured.
Application examples 17 to 18
The difference between the boswellia extract and the application example 1 is that the bicontinuous phase extract is used in different conditions, and the specific correspondence is shown in the table below.
Table: use condition comparison table of bicontinuous phase extracting solution in application examples 17-18
| Group of | Bicontinuous phase extract |
| Application example 17 | From example 15 |
| Application example 18 | From example 16 |
The mastic extracts prepared in application examples 17 to 18 were extracted, and the contents of the components were measured according to the above measurement procedures and measurement criteria, and the contents of the components were examined to be the same as those of application example 1, so that the selection of the vegetable fat had no obvious influence on the extraction rate of the bicontinuous phase liquid, but was advantageous for the formation of the bicontinuous phase liquid and the improvement of the stability of the bicontinuous phase liquid.
The present embodiment is only for explanation of the present application and is not to be construed as limiting the present application, and modifications to the present embodiment, which may not creatively contribute to the present application as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present application.
Claims (3)
1. The application of bicontinuous phase extracting solution in the frankincense extracting method comprises an A phase, a B phase and a C phase;
the phase A consists of the following components in parts by weight:
20-25 parts of deionized water;
32-45 parts of polyalcohol;
1-5 parts of sodium surfactin;
the polyalcohol is one or more of 1, 3-butanediol and dipropylene glycol;
the phase B consists of the following components in parts by weight:
12-25 parts of polyglycerols;
2-10 parts of alkanes;
2-10 parts of ester grease;
the polyglycerin oil is one or more of polyglycerin-5 trioleate and polyglycerin-10 diisostearate;
the alkane is squalane, hydrogenated polyisobutene, hydrogenated polydecene, C 12-19 Alkane, month hydrogenation C 6-14 One or more of olefin polymers and isododecane;
the ester grease is medium chain triglyceride grease;
the phase C consists of the following components in parts by weight:
1-5 parts of vegetable oil;
the frankincense extraction method using the bicontinuous phase extracting solution comprises the following steps:
adding Olibanum (at a feed liquid ratio of 1 (10-20)) into bicontinuous phase extractive solution, extracting at 50-85deg.C for 0.5-3 hr, filtering, and removing residue to obtain Olibanum extract.
2. The use of the bicontinuous phase extract according to claim 1, wherein the vegetable oil is one or more of jojoba seed oil, shea butter, sweet almond oil, castor oil, grape seed oil, sunflower seed oil, white pool seed oil, linseed oil.
3. Use of a bicontinuous phase extract according to any of claims 1-2 in a method of extraction of mastic gum, characterized in that the method of preparation of bicontinuous phase extract comprises the steps of:
a1, heating the phase A to 80-85 ℃ respectively, and stirring for 15-30min at 300-500 r/min; heating the phase B to 80-85 ℃, stirring for 5-10min at 300-500r/min, and uniformly mixing;
a2, mixing the phase B and the phase A, and stirring for 2-5min at 1000-1200r/min to obtain an AB mixed phase;
and A3, finally cooling the AB mixed phase to 50-60 ℃, adding the C phase, stirring for 5-10min at 300-500r/min, and uniformly stirring to obtain the bicontinuous phase extract.
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| CN109363975A (en) * | 2018-12-17 | 2019-02-22 | 广州诗美化妆品有限公司 | A kind of preparation method and application of Olibanum extract used for cosmetic |
| CN111658572A (en) * | 2019-03-08 | 2020-09-15 | 上海黛芊化妆品有限公司 | Skin care compositions |
| CN112057406A (en) * | 2020-10-26 | 2020-12-11 | 上海典雅生物科技有限公司 | Bicontinuous clear and thorough cleansing oil and preparation method thereof |
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| CN109363975A (en) * | 2018-12-17 | 2019-02-22 | 广州诗美化妆品有限公司 | A kind of preparation method and application of Olibanum extract used for cosmetic |
| CN111658572A (en) * | 2019-03-08 | 2020-09-15 | 上海黛芊化妆品有限公司 | Skin care compositions |
| CN112057406A (en) * | 2020-10-26 | 2020-12-11 | 上海典雅生物科技有限公司 | Bicontinuous clear and thorough cleansing oil and preparation method thereof |
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