CN109363975A - A kind of preparation method and application of Olibanum extract used for cosmetic - Google Patents
A kind of preparation method and application of Olibanum extract used for cosmetic Download PDFInfo
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- CN109363975A CN109363975A CN201811541274.6A CN201811541274A CN109363975A CN 109363975 A CN109363975 A CN 109363975A CN 201811541274 A CN201811541274 A CN 201811541274A CN 109363975 A CN109363975 A CN 109363975A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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Abstract
The invention discloses the preparation methods and application of a kind of Olibanum extract used for cosmetic.Method includes the following steps: (1) crushes dry olibanum, frankincense is obtained；(2) matrix oil is added into frankincense, ultrasonic extraction is carried out under the conditions of 40~60 DEG C, obtains extracting solution；Then extracting solution is separated by solid-liquid separation, collects liquid, obtains centrifugate；Antioxidant is added into centrifugate again, obtains Olibanum extract.The method of the present invention has easy to operate, with short production cycle, product and dissolubility good; activity of fighting against senium is high; the advantages that stability is good; overcome that traditional extraction rate is low, active constituent easily decomposes inactivation simultaneously; overcritical high production cost, solvent extraction have the shortcomings that residual, and Olibanum extract boswellic acids substance rich in obtained has the effect of obviously desalinating microgroove; natural antidotal agent addition be can be used as in cosmetics, be suitable for large-scale production.
Description
Technical field
The present invention relates to daily cosmetics field, in particular to a kind of preparation method of Olibanum extract used for cosmetic with answer
With.
Background technique
Olibanum is dry gum resin, is in small shape mamillary, teardrop shaped particle or irregular fritter more.It is littoral to be distributed Red sea
To Libya, the Sudan, Turkey and other places, main product is in the Somalia and Ethiopia of Red sea bank.Its main function is promoting blood circulation
The effects of analgesic, myogenic is antipruritic, removing toxicity for detumescence, Compendium of Material Medica are recorded olibanum fragrance and are altered, and the heart channel of Hang-Shaoyin is entered, analgesic therapy of invigorating blood circulation, therefore are carbuncle
Subcutaneous ulcer sore, pain in the chest and abdomen key medicine.
The traditional extracting method of olibanum active constituent such as steam distillation etc. requires high temperature, is easy agglomeration, so as to cause
Effective component extraction is incomplete, extraction efficiency is low；And supercritical CO2Extraction the shortcomings that avoiding conventional method, has certain excellent
Gesture can extract more effective components, but since supercritical carbon dioxide extract equipment is expensive, it is raw to be unable to continous way
The reasons such as production, production efficiency are low, and production cost is higher, it is difficult to promote, while its active constituent of means of supercritical extraction also mentions simultaneously
A large amount of macromolecular lipid like material is taken, there is biggish limitation in actual use.Chinese patent ZL201180049685.3
With adjusting the multistep processes extractions such as acid ion alkali ion exchange to prepare Olibanum extract after the extraction of alcohol extracting organic solvent, to obtain molten in polarity
Soluble acidic fraction in agent and nonpolar solvent repairs medicine for treating apoplexy or surgical wound.But due to being that alcohol refers to
Organic solvent extraction, there are the remaining problems of organic solvent.
Cosmetics have special requirement to raw material, safety as a kind of specialities for being mainly used for beautifying people's appearance
For space of top prominence, but its effect is taken into account simultaneously, especially influence of the size of raw material effective component molecular weight to effect itself
It is extremely important.In general, raw molecule amount is smaller, and Transdermal absorption ability is stronger, and functional component can be penetrated more faster
The through inside of human body of cuticula works.Therefore, it is highly desirable the special nature for daily use chemicals industry, one kind is developed and is suitble to be somebody's turn to do
The Olibanum extract that industry uses.
Summary of the invention
The primary purpose of the present invention is that the shortcomings that overcoming the prior art and deficiency, provide a kind of olibanum extraction used for cosmetic
The preparation method of object.
Another object of the present invention is to provide the Olibanum extracts used for cosmetic that the method is prepared.
Another object of the present invention is to provide the applications of the Olibanum extract used for cosmetic.
The purpose of the invention is achieved by the following technical solution: a kind of preparation method of Olibanum extract used for cosmetic, packet
Include following steps:
(1) olibanum after drying is crushed, obtains frankincense；
(2) matrix oil is added into frankincense obtained in step (1), ultrasound is carried out under the conditions of 40~60 DEG C and is mentioned
It takes, obtains extracting solution；Then extracting solution is separated by solid-liquid separation, collects liquid, obtains centrifugate；Antioxygen is added into centrifugate again
Agent obtains Olibanum extract.
Drying described in step (1) is 60 DEG C of constant pressure and dries.
Frankincense described in step (1) is the frankincense of 100~200 mesh.
Crushing described in step (1) is that cryogenic mechanical crushes under the conditions of -30 DEG C~0 DEG C；Preferably -30 DEG C~-
Cryogenic mechanical crushes under the conditions of 15 DEG C.
It is described to be mechanically pulverized to be crushed using carragheen pulverizer；More preferably use DFJ type carragheen pulverizer
It is crushed.
Matrix oil described in step (2) includes White Mineral Oil, saualane, ethylhexyl palmitate, octanoic acid/certain herbaceous plants with big flowers acid triglycerin
The mixture of one or more of ester, olive oil, wheat-germ oil and sunflower oil；Preferably sunflower oil, olive
Oil, saualane, ethylhexyl palmitate and octanoic acid/certain herbaceous plants with big flowers acid glyceryl ester are the mixture that 1:1 is mixed to get, white mine by weight
Oil and wheat-germ oil are the mixture that 1:4 is mixed to get by weight or White Mineral Oil, saualane and olive oil are by weight
The mixture that 3:2:1 is mixed to get.
The sunflower oil is cosmetics-stage sunflower oil.
The weight ratio of frankincense described in step (2) and matrix oil be 1:1~30 (i.e. solid-liquid ratio be 1g:1~
30g)。
Separation of solid and liquid described in step (2) is to be separated by solid-liquid separation by the way of centrifugation；It is centrifuged speed be
2000~5000rpm.
The condition of ultrasonic extraction described in step (2) are as follows: 20~50KHz of supersonic frequency, 1000~3000W of power, when
Between 10~60min；It is preferred that are as follows: 30~50KHz of supersonic frequency, 2000~3000W of power, 20~60min of time.
Antioxidant described in step (2) is 2,6- toluene di-tert-butyl phenol.
The additive amount of antioxidant described in step (2) is account for centrifugate weight 0.1%.
A kind of Olibanum extract is prepared by method described in any of the above embodiments.
Application of the Olibanum extract in anti-aging or cosmetic field.
The additive amount of the Olibanum extract accounts for the 0.5~50% of cosmetics weight；Preferably account for the 3 of cosmetics weight
~5%；More preferably account for the 5% of cosmetics weight.
The cosmetics are preferably antisenile series cosmetics, including cream emulsion, film powder class, thermophilic curry class, aerosol
Agent class and muffin class etc..
A kind of facial mask liquid containing Olibanum extract, the component including following percentage:
A phase:
Polyvinyl alcohol 13%；
Span-20 1%；
B phase:
Above-mentioned Olibanum extract 3~5%；
Sodium benzoate 0.25%；
C phase:
Carboxymethyl cellulose 2%；
Glycerol 5%；
Water complements to 100%.
The B phase also contains essence.
The essence is preferably lemon extract.
The additive amount of the essence is preferably account for the facial mask liquid weight containing Olibanum extract 0.1%.
Water in the C phase is preferably distilled water.
The preparation method of the facial mask liquid containing Olibanum extract, includes the following steps:
(1) by the polyvinyl alcohol in A phase and after Span-20 stirs and evenly mixs, A phase mixture is obtained；
(2) it is stirred evenly after mixing each component in C phase, is then warming up to 80 DEG C and is stirred for 2 hours, then added
Enter into A phase mixture, stir evenly, the component in B phase is added after being cooled to 40 DEG C, stirring makes its mixing equal for 0.5 hour
It is even, obtain the facial mask liquid containing Olibanum extract.
A kind of skin cream containing Olibanum extract, the component including percentage:
A phase:
Paraffin 4%；
Polyethylene glycol stearate 8%；
Vaseline 4%；
Lanolin 8%；
Atoleine 6%；
Butyl stearate 6%；
B phase:
Above-mentioned Olibanum extract 3~5%；
C phase:
Phosphoric acid cetyl ester sylvite 8%；
Glycerol tristearate 3%；
Borax 0.7%；
Methylparaben 0.2%；
Water complements to 100%.
Lanolin in the A phase is oil-soluble lanolin.
The B phase also contains essence.
The essence is preferably lemon extract.
The additive amount of the essence is preferably account for the skin cream weight containing Olibanum extract 0.1%.
The additive amount of Olibanum extract is preferably to account for the skin cream weight containing Olibanum extract in the B phase
3%.
Water in the C phase is preferably distilled water.
The preparation method of the skin cream containing Olibanum extract includes the following steps: for A phase each component to be uniformly mixed
And 80~85 DEG C are heated to, obtain A phase mixture；Then C phase each component is uniformly mixed and is heated to 80~85 DEG C, obtain C
Phase mixture；A phase mixture is added in C phase mixture again, stirring is emulsified, and is added in B when temperature is down to 40 DEG C
Component, stir 30min, obtain the skin cream containing Olibanum extract.
A kind of plumule moisturizing lotion containing Olibanum extract, the component including percentage:
Wheat-germ oil 12%；
Beeswax 3%；
Lanolin 2%；
Stearic acid 3%；
Propylene glycol 1%；
Triethanolamine 1.5%；
Above-mentioned Olibanum extract 3~5%；
Phenoxyethanol 0.35%；
BHT (2,6 di tert butyl 4 methyl phenol) 0.1%；
Water complements to 100%.
The lanolin is oil-soluble lanolin.
The additive amount of the Olibanum extract is preferably to account for the plumule moisturizing lotion weight containing Olibanum extract
5%.
The plumule moisturizing lotion containing Olibanum extract also contains essence.
The essence is preferably lemon extract.
The additive amount of the essence is preferably account for the plumule moisturizing lotion weight containing Olibanum extract 0.1%.
The preparation method of the plumule moisturizing lotion containing Olibanum extract, includes the following steps:
(I) first that wheat-germ oil, beeswax, lanolin, stearic acid and BHT (2,6 di tert butyl 4 methyl phenol) is mixed
It closes uniformly, is stirred 0.5 hour after then heating to 85 DEG C, obtain mixture A；
(II) propylene glycol, Phenoxyethanol and triethanolamine are added to the water after mixing, stirring is added to after ten minutes
In mixture A obtained in step (1), 85 DEG C are continued stirring 1 hour, obtain mixture B；
(III) when mixture B obtained in step (2) being cooled to 40 DEG C, remaining ingredient is added, stirs 0.5 hour, obtains
To the plumule moisturizing lotion containing Olibanum extract.
Application of the Olibanum extract in the drug that preparation promotes cell Proliferation.
The cell is preferably T lymphocyte.
The present invention has the following advantages and effects with respect to the prior art:
1, the present invention mainly prepares olibanum used for cosmetic using cosmetic base oil combination ultrasonic cryogenic extractive technique and extracts
High efficiency extraction under ultrasonic cryogenic and cosmetic base oil are had effective component knot in the dissolution olibanum for specificity by the technique of object
It closes and obtains olibanum active constituent used for cosmetic, have easy to operate, with short production cycle, product and dissolubility good, the molecular weight of product
It is reasonably distributed, the advantages that activity of fighting against senium is high, and stability is good, while overcoming that traditional extraction rate is low, active constituent easily decomposes mistake
Living, overcritical high production cost, solvent extraction have the shortcomings that residual.Extraction process of the present invention is simple, for expanding olibanum medicinal material
Have cosmetic industry using significant.
2, method of the invention can high efficiency be made effective component enrichment Olibanum extract, be highly suitable for scale metaplasia
It produces and is suitable for adding application in cosmetics.Olibanum extract boswellic acids substance rich in produced by the present invention, has
Obvious desalination microgroove, promotes facial remarks circulating effect, can be used as natural antidotal agent addition in cosmetics.
Detailed description of the invention
Fig. 1 is the high-efficient liquid phase chromatogram of α-masticinic acid and beta boswellic acid in Olibanum extract.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus；Unless
It illustrates, agents useful for same and raw material of the present invention can pass through commercially available acquisition.Medicinal material used in the present invention is purchased from Guangzhou medicinal material
Prepared slices of Chinese crude drugs factory of company, Gum Olibanum Ethiopia meet Chinese Pharmacopoeia standard requirements.
Embodiment 1
Olibanum medicinal material after (the same below) drying of 60 DEG C of normal pressures is set in DFJ type carragheen pulverizer, under the conditions of -15 DEG C
Feed powder is broken into 140 mesh powder, takes the above-mentioned smashed olibanum raw material of 1kg, and 20kg saualane is added, is stirring evenly and then adding into ultrasound
It is extracted in extractor, extraction time is 20 minutes, supersonic frequency 40KHz, power 2000kw, Extracting temperature 60
DEG C, the dregs of a decoction are removed with 3500rpm link-suspended basket centrifuge after extraction, 2,6- toluene di-tert-butyl phenol is added in centrifugate
(0.1% (w/w) that its additional amount accounts for centrifugate) is to get Olibanum extract.
Embodiment 2
Olibanum medicinal material after drying is set in DFJ type carragheen pulverizer, feed powder is broken into 200 mesh powder under the conditions of -30 DEG C
End takes the above-mentioned smashed olibanum raw material of 1kg, and 20kg ethylhexyl palmitate and octanoic acid/certain herbaceous plants with big flowers acid triglyceride mixtures are added
(weight ratio 1:1) is stirring evenly and then adding into ultrasonic extraction tank and extracts, and extraction time is 50 minutes, and supersonic frequency is
35KHz, power 2500kw, Extracting temperature are 50 DEG C, remove the dregs of a decoction with 3000rpm link-suspended basket centrifuge after extraction, from
2,6- toluene di-tert-butyl phenol (0.1% (w/w) that its additional amount accounts for centrifugate) is added in heart liquid to get Olibanum extract.
Embodiment 3
Olibanum medicinal material after drying is set in DFJ type carragheen pulverizer, feed powder is broken into 160 mesh powder under the conditions of -20 DEG C
End takes the above-mentioned smashed olibanum raw material of 1kg, is added 10kg sunflower oil (cosmetics-stage), is stirring evenly and then adding into ultrasound and mentions
It taking in tank and extracts, extraction time is 60 minutes, supersonic frequency 30KHz, power 3000kw, and Extracting temperature is 60 DEG C,
The dregs of a decoction are removed with 2500rpm link-suspended basket centrifuge after extraction, 2,6- toluene di-tert-butyl phenol is added in centrifugate, and (it adds
Enter 0.1% (w/w) that amount accounts for centrifugate) to get Olibanum extract.
Embodiment 4
Olibanum medicinal material after drying is set in DFJ type carragheen pulverizer, feed powder is broken into 100 mesh powder under the conditions of -5 DEG C
End takes the above-mentioned smashed olibanum raw material of 1kg, and 15kg White Mineral Oil is added and wheat-germ oil mixes object solvent (weight ratio 1:4), stirs
It is added in ultrasonic extraction tank and extracts after mixing uniformly, extraction time is 30 minutes, supersonic frequency 50KHz, and power is
3000kw, Extracting temperature are 60 DEG C, remove the dregs of a decoction with 2000rpm centrifuge after extraction, 2,6-, bis- uncle is added in centrifugate
Butyl p-cresol (0.1% (w/w) that its additional amount accounts for centrifugate) is to get Olibanum extract.
Embodiment 5
Olibanum original after drying is set in DFJ type carragheen pulverizer, feed powder is broken into 180 mesh powder under the conditions of -30 DEG C,
Take the above-mentioned smashed olibanum raw material of 1kg, be added 30kg White Mineral Oil, saualane and olive oil mixed solvent (weight ratio=3:2:
1) it, is stirring evenly and then adding into ultrasonic extraction tank and extracts, extraction time is 50 minutes, supersonic frequency 40KHz, and power is
2000kw, Extracting temperature are 40 DEG C, remove the dregs of a decoction with 4000rpm centrifuge after extraction, 2,6-, bis- uncle is added in centrifugate
Butyl p-cresol (0.1% (w/w) that its additional amount accounts for centrifugate) is to get Olibanum extract.
Embodiment 6
Olibanum original after drying is set in DFJ type carragheen pulverizer, feed powder is broken into 200 mesh powder under the conditions of -20 DEG C,
The above-mentioned smashed olibanum raw material of 1kg is taken, is added 20kg olive oil (cosmetics-stage), is stirring evenly and then adding into ultrasonic extraction tank
It extracts, extraction time is 60 minutes, supersonic frequency 50KHz, power 2000kw, and Extracting temperature is 60 DEG C, has been extracted
The dregs of a decoction are removed with 5000rpm link-suspended basket centrifuge after finishing, 2,6- toluene di-tert-butyl phenol is added in centrifugate, and (its additional amount accounts for
0.1% (w/w) of centrifugate) to get Olibanum extract.
Comparative example 1
Ethyl alcohol is added after olibanum pulverizing medicinal materials to prepare in conventional heating extraction process:
Olibanum original after drying is set in DFJ type carragheen pulverizer, feed powder is broken into 200 mesh powder under the conditions of -20 DEG C,
The above-mentioned smashed olibanum raw material of 1kg is taken, 20kg70% (w/w) ethanol solution is added, is stirring evenly and then adding into and is heated to reflux tank
In extract, extraction time be 60 minutes, 80 DEG C, after extraction with 5000rpm link-suspended basket centrifuge remove the dregs of a decoction to get
Olibanum extract.
Embodiment 7
The assay of masticinic acid
Chromatographic condition: Hypersil ODS2-C18 chromatographic column (4.6mm × 150mm, 5 μm)；
Mobile phase: 0.1% (w/v) phosphate aqueous solution (A)-acetonitrile (B)；
Type of elution: gradient elution
0~10min, 50%B
10~20min, 60%~80%B
20~35min, 80%B
Detection wavelength 210nm, 30 DEG C of column temperature, flow velocity 1.0mL/min.
The preparation of reference substance solution: taking α-masticinic acid respectively, and beta boswellic acid reference substance is appropriate, accurately weighed, adds methanol system
It is respectively 0.1,0.3,0.5,0.7,0.9,1.2mg/ml mixed reference substance solution at mass concentration.
The preparation of test solution: precision draws each test liquid, and (i.e. Examples 1 to 5 and the olibanum of comparative example preparation is extracted
Object) 1ml to 10ml volumetric flask, adds methanol constant volume, shakes up, filter through 0.45 μm of miillpore filter, obtain each test solution.Measurement knot
Fruit is as shown in table 1.The high-efficient liquid phase chromatogram of α-masticinic acid and beta boswellic acid is as shown in Figure 1 in Olibanum extract.
Each embodiment assay result of table 1
Embodiment 8
A kind of facial mask liquid containing Olibanum extract, is grouped as by the group of following percentage:
A phase:
Polyvinyl alcohol 13%；
Span-20 1%；
B phase:
The Olibanum extract 5% prepared in embodiment 2；
Sodium benzoate 0.25%；
Lemon extract (fragrant kindness) 0.1%；
C phase:
Carboxymethyl cellulose 2%；
Glycerol 5%；
Distilled water complements to 100%.
Preparation method:
By the polyvinyl alcohol in A phase and after Span-20 stirs and evenly mixs, A phase mixture is obtained, for use；
C phase is stirred evenly, is warming up to 80 DEG C or so, is stirred 2 hours, it is rear that A phase mixture is added, it stirs evenly, is cooled to 40 DEG C of left sides
B phase materials are added behind the right side, is stirred 0.5 hour and gets product.
Embodiment 9
A kind of skin cream containing Olibanum extract, is grouped as by the group of following percentage:
A phase:
Paraffin 4%
Polyethylene glycol stearate 8%
Vaseline 4%
Lanolin (rising sun star, oil-soluble lanolin) 8%
Atoleine 6%
Butyl stearate 6%；
B phase:
Olibanum extract 3% is prepared in embodiment 3
Lemon extract (fragrant kindness) 0.1%；
C phase:
Phosphoric acid cetyl ester sylvite 8%
Glycerol tristearate 3%
Borax 0.7%
Methylparaben 0.2%
Distilled water complements to 100%.
[preparation method] mixes A phase component, is heated to 80~85 DEG C.C phase component is mixed, is heated to 80~85 DEG C, by A phase
Component is added in C phase component, and stirring is emulsified, and is added B component when temperature is down to 40 DEG C, mix 30min to get.
Embodiment 10
A kind of plumule moisturizing lotion containing Olibanum extract, is grouped as by the group of following percentage:
Wheat-germ oil 12%
Beeswax 3%
Lanolin (rising sun star, oil-soluble lanolin) 2%
Stearic acid 3%
Propylene glycol 1%
Triethanolamine 1.5%
The Olibanum extract 5% prepared in embodiment 1
Phenoxyethanol 0.35%
BHT (2,6- di-tert-butyl-4-methy phenol) 0.1%
Lemon extract (fragrant kindness) 0.1%
Purified water complements to 100%.
[preparation method]
(1) first wheat-germ oil, beeswax, lanolin, stearic acid and BHT are mixed, it is small that 0.5 is stirred after being warming up to 85 DEG C
When, obtain mixture A；
(2) propylene glycol and Phenoxyethanol and triethanolamine are added in pure water after mixing, stirring adds after ten minutes
Enter into mixture A obtained in step (1), 85 DEG C are continued stirring 1 hour, obtain mixture B；
(3) when mixture B obtained in step (2) being cooled to 40 DEG C, Olibanum extract and essence, stirring 0.5 is added
Hour to get.
Embodiment 11
Stability test
9 product of Example, the another comparative example for preparing is same as Example 9, and unique difference is to be made in comparative example 1
Standby Olibanum extract substitutes the Olibanum extract in former embodiment 3.According under QB/T1857-2013 moisturizing cream normal term into
Row 7 days it is heat-resisting it is cold-resistant alternately test, sense organ and measure its pH value evaluation steadiness, as a result it is as shown in the table.
2 stability test result of table
| 7 days stability test results | |
| Embodiment 9 | Fine and smooth uniformly without water-oil separating phenomenon, lotion, odor type meets regulation, pH variation ± 0.2. |
| Comparative example | There is within 5 days water-oil separating, color is deepened. |
Embodiment 12
Cell proliferation experiment method verifies Olibanum extract anti-aging effects.
Experimental material and reagent: 96 porocyte culture plates, CO2Incubator, alcolhol burner, micropipettor, microplate reader, CCK-
8 kits, T lymphocyte, the Olibanum extract prepared in embodiment 5 are diluted to 0.5%, 1%, 3%, 5% with PBS solution
(W/W) it is used as test sample.
Experimental procedure:
1. the good T lymphocyte of growth conditions (purchased from Shanghai Yan Jin Biotechnology Co., Ltd) is taken to be prepared into 1*104/
Ml~1*105The cell suspension of/ml concentration, every hole take 100 μ l to be added in 96 porocyte culture plates.
2. 10 μ l test samples are added in step 1 culture hole, in 37 DEG C incubator culture 4 hours, every test sample parallel 3
It is secondary.
3. taking 10 μ l to be added in step 2 culture hole the CCK-8 solution in kit, continue to be incubated in 37 DEG C of incubators
2 hours.
4. taking step 3 to be incubated for measures absorbance in 450nm, its cultivation effect is calculated.The results are shown in Table 3.
3 Cell proliferation results of table
| Olibanum extract concentration | 0.5% | 1% | 3% | 5% |
| Proliferation rate | 18% | 35% | 54% | 98% |
As the result is shown with the increase of Olibanum extract concentration, the proportional ascendant trend of proliferation rate.The experiment is tentatively said
Bright, Olibanum extract has certain anti-aging effects.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of preparation method of Olibanum extract used for cosmetic, which comprises the following steps:
(1) olibanum after drying is crushed, obtains frankincense；
(2) matrix oil is added into frankincense obtained in step (1), ultrasonic extraction is carried out under the conditions of 40~60 DEG C, is obtained
To extracting solution；Then extracting solution is separated by solid-liquid separation, collects liquid, obtains centrifugate；Antioxidant is added into centrifugate again, obtains
Olibanum extract.
2. the preparation method of Olibanum extract used for cosmetic according to claim 1, it is characterised in that:
Crushing described in step (1) is that cryogenic mechanical crushes under the conditions of -30 DEG C~0 DEG C；
Matrix oil described in step (2) is White Mineral Oil, saualane, ethylhexyl palmitate, octanoic acid/certain herbaceous plants with big flowers acid glyceryl ester, olive
The mixture of one or more of olive oil, wheat-germ oil and sunflower oil；
Antioxidant described in step (2) is 2,6- toluene di-tert-butyl phenol.
3. the preparation method of Olibanum extract used for cosmetic according to claim 2, it is characterised in that:
Crushing described in step (1) is that cryogenic mechanical crushes under the conditions of -30 DEG C~-15 DEG C；
Matrix oil described in step (2) is sunflower oil, olive oil, saualane, ethylhexyl palmitate and octanoic acid/certain herbaceous plants with big flowers acid three
Glyceride is the mixture that 1:1 is mixed to get by weight, and White Mineral Oil and wheat-germ oil are what 1:4 was mixed to get by weight
Mixture or White Mineral Oil, saualane and olive oil are the mixture that 3:2:1 is mixed to get by weight.
4. the preparation method of Olibanum extract used for cosmetic according to claim 1, it is characterised in that:
The weight ratio of frankincense described in step (2) and matrix oil is 1:1~30；
The additive amount of antioxidant described in step (2) is account for centrifugate weight 0.1%.
5. the preparation method of Olibanum extract used for cosmetic according to claim 1, it is characterised in that:
Drying described in step (1) is 60 DEG C of constant pressure and dries；
Frankincense described in step (1) is the frankincense of 100~200 mesh；
Separation of solid and liquid described in step (2) is to be separated by solid-liquid separation by the way of centrifugation；It is centrifuged speed be 2000~
5000rpm；
The condition of ultrasonic extraction described in step (2) are as follows: 20~50KHz of supersonic frequency, 1000~3000W of power, time 10
~60min.
6. a kind of Olibanum extract, it is characterised in that: be prepared by the described in any item methods of Claims 1 to 5.
7. application of the Olibanum extract as claimed in claim 6 in anti-aging or cosmetic field, it is characterised in that:
The cosmetics are antisenile series cosmetics；
The antisenile series cosmetics are cream emulsion, film powder class, thermophilic curry class, aerosol class or muffin class cosmetics.
8. a kind of facial mask liquid containing Olibanum extract, it is characterised in that: the component including following percentage:
A phase:
Polyvinyl alcohol 13%；
Span-20 1%；
B phase:
Olibanum extract 3~5% as claimed in claim 6；
Sodium benzoate 0.25%；
C phase:
Carboxymethyl cellulose 2%；
Glycerol 5%；
Water complements to 100%；
The B phase also contains essence；
The additive amount of the essence is account for the facial mask liquid weight containing Olibanum extract 0.1%；
The preparation method of the facial mask liquid containing Olibanum extract, includes the following steps:
(1) by the polyvinyl alcohol in A phase and after Span-20 stirs and evenly mixs, A phase mixture is obtained；
(2) it is stirred evenly after mixing each component in C phase, is then warming up to 80 DEG C and is stirred for 2 hours, be then added into
It in A phase mixture, stirs evenly, the component in B phase is added after being cooled to 40 DEG C, stirring is uniformly mixed it, obtains
To the facial mask liquid containing Olibanum extract.
9. a kind of skin cream containing Olibanum extract, it is characterised in that: the component including percentage:
A phase:
Paraffin 4%；
Polyethylene glycol stearate 8%；
Vaseline 4%；
Lanolin 8%；
Atoleine 6%；
Butyl stearate 6%；
B phase:
Olibanum extract 3~5% as claimed in claim 6；
C phase:
Phosphoric acid cetyl ester sylvite 8%；
Glycerol tristearate 3%；
Borax 0.7%；
Methylparaben 0.2%；
Water complements to 100%；
Lanolin in the A phase is oil-soluble lanolin；
The B phase also contains essence；
The additive amount of the essence is account for the skin cream weight containing Olibanum extract 0.1%；
The preparation method of the skin cream containing Olibanum extract includes the following steps: that A phase each component is uniformly mixed and is added
Heat obtains A phase mixture to 80~85 DEG C；Then C phase each component is uniformly mixed and is heated to 80~85 DEG C, obtained C phase and mix
Close object；A phase mixture is added in C phase mixture again, stirring is emulsified, and is added in B phase when temperature is down to 40 DEG C
Component stirs 30min, obtains the skin cream containing Olibanum extract.
10. a kind of plumule moisturizing lotion containing Olibanum extract, it is characterised in that: the component including percentage:
Wheat-germ oil 12%；
Beeswax 3%；
Lanolin 2%；
Stearic acid 3%；
Propylene glycol 1%；
Triethanolamine 1.5%；
Olibanum extract 3~5% as claimed in claim 6；
Phenoxyethanol 0.35%；
2,6 di tert butyl 4 methyl phenol 0.1%；
Water complements to 100%；
The lanolin is oil-soluble lanolin；
The plumule moisturizing lotion containing Olibanum extract also contains essence；
The additive amount of the essence is account for the plumule moisturizing lotion weight containing Olibanum extract 0.1%；
The preparation method of the plumule moisturizing lotion containing Olibanum extract, includes the following steps:
(I) first by wheat-germ oil, beeswax, lanolin, stearic acid and 2,6- di-tert-butyl-4-methy phenol is uniformly mixed, so
After be warming up to 85 DEG C after stir 0.5 hour, obtain mixture A；
(II) propylene glycol, Phenoxyethanol and triethanolamine are added to the water after mixing, stirring is added to step after ten minutes
(1) in mixture A obtained in, 85 DEG C are continued stirring 1 hour, obtain mixture B；
(III) when mixture B obtained in step (2) being cooled to 40 DEG C, remaining ingredient is added, stirs 0.5 hour, is contained
The plumule moisturizing lotion of Olibanum extract.
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| CN114525175B (en) * | 2021-12-31 | 2023-09-29 | 上海澄穆化妆品有限公司 | Bicontinuous phase extracting solution, preparation method thereof and extraction method applied to frankincense |
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