CN114516905B - 植物光合调控基因tl7及其蛋白与应用 - Google Patents
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Abstract
本发明公开了一种植物基因TL7的应用,属于生物技术领域。植物基因TL7是一类在植物中高度保守的基因,它包括了所有的植物物种中的同源基因。这些结果表明TL7基因是植物光合作用所必需的,是响应光逆境重要的因子,通过控制TL7基因的表达量可以调控植物的生长。因此本发明为提高植物光能利用率,增加作物大田产量,提高植物响应光逆境能力提供了一条新途径,在农业现代化领域具有重要的作用。
Description
技术领域
本发明属于生物技术领域,涉及一种植物光合调控基因TL7及其蛋白与应用。
背景技术
光是影响植物生长最重要的因子之一,光照强度对于植物的生长发育、生物量的积累及次生代谢产物的含量具有极大的影响作用,因而研究光照对于光合作用与次生代谢产物及其产量的关系仍然是当今植物生物学领域的重大研究课题之一。而有关光适应、光调节的生物学机理是其主要的学术热点。
光合作用是地球上最重要的化学反应,光合作用产生的氧气和碳水化合物是地球生物必不可少的生存物质,而叶绿体是最重要的光合反应细胞器。叶绿体在结构上可分为:叶绿体双层膜、基质、类囊体膜和类囊体腔。高等植物的光反应是在叶绿体的类囊体膜上进行的,类囊体上具有将光能转换成化学能所需要的光系统II(Photosystem II,PSII)、细胞色素b6f复合体、光系统I(Photosystem I,PSI)、ATP合酶复合体,以及各种电子载体等。
植物依赖阳光进行光合作用,但必须应对光强和光质的不断变化。事实上,植物用多种生理机制来适应自然界不断变化的光照条件(Rascher U,Nedbal L.Dynamics ofphotosynthesis in fluctuating light[J].Current Opinion in Plant Biology,2006,9(6):671-678)。这包括长期适应、短期调节和光抑制、光保护等。
植物短期通过调节PSI与PSII之间光激发能的分布来适应波动的光强,即状态转换(Bellafiore S,Bameche F,Peltier G,et al.State transitions and lightadaptation require chloroplast thylakoid protein kinase STN7.[J].Nature,2005,433(7028):892-895)。这个过程通过STN7激酶和PPH1/TAP38磷酸酶的可逆磷酸化,使捕光复合体II(Light Harvesting Complex II,LHCII)在PSI与PSII之间移动(Shapiguzov A,Ingelsson B,Samol I,et al.The PPH1 phosphatase is specifically involved inLHCII dephosphorylation and state transitions in Arabidopsis[J].Proc NatlAcad Sci USA,2010,107(10):4782-4787.DOI:10.1073/pnas.0913810107.;Pribil M,Pesaresi P,Hertle A,et al.Role of plastid protein phosphatase TAP38 in LHCIIdephosphorylation and thylakoid electron flow[J].PLoS Biol,2010,8(1):e1000288.DOI:10.1371/journal.pbio.1000288.)。
光保护是另一个重要的短期调节机制(Li X P,Bjrkman O,Shih C,et al.Apigment-binding protein essential for regulation of photosynthetic lightharvesting.[J].Nature,2000,403(6768):391-395.)。植物通过非光化学猝灭(non-photochemical quenching,NPQ)将多余的光能以热能的形式耗散(Ruban A V,Berera R,Ilioaia C,et al.Identification of a mechanism of photoprotective energydissipation in higher plants[J].Nature,2007,450(7169):575-578.DOI:10.1038/nature06262),并通过细胞色素b6f控制质子流动速率等方法,在变化的光照条件下保护PSI(Suorsa M,S,Grieco M,et al.PROTON GRADIENT REGULATION5 is essentialfor proper acclimation of Arabidopsis photosystem I to naturally andartificially fluctuating light conditions[J].Plant Cell,2012,24(7):2934-2948.DOI:10.1105/tpc.112.097162.)。
光抑制是植物在强光下吸收光能超过光合系统所能利用的数量时,所出现的光合效率下降的现象(Long S P,Humphries S,Falkowski P G.Photoinhibition ofPhotosynthesis in Nature[J].Ann.rev.plant Physiol.plant Mol.biol,1994,45(1):633-662.)。光抑制会损伤PSII反应中心的D1蛋白。D1在PSII的电子传递中起着电子供体和电子受体的双重作用,它具有P680反应中心分子的原初电子受体脱镁叶绿素和质体醌QB的结合部位,质体醌QB为质体醌库的质子化接受电子。
植物有强大的修复系统可以替换受损的PSII反应中心蛋白(Takahashi S,BadgerM R.Photoprotection in plants:a new light on photosystem II damage[J].TrendsPlant Sci,2011,16(1):53-60.DOI:10.1016/j.tplants.2010.10.001.),并且有一组蛋白可以促进这一长期修复的过程(Lu Y.Identification and Roles of Photosystem IIAssembly,Stability,and Repair Factors in Arabidopsis[J].Front Plant Sci,2016,7:168.DOI:10.3389/fpls.2016.00168)。
其他的长期适应包括调节光合相关基因的表达(Chi W,Sun X,ZhangL.Intracellular signaling from plastid to nucleus[J].Annu Rev PlantBiol.2013,64:559-582.)。植物还可以调节叶绿素蛋白复合体的相对含量,即通过PSII、PSI的反应中心和光合单位的大小及其它如细胞色素、质体醌、质体蓝素和铁氧还蛋白等电子载体来完成其对光强变化的反应,以优化光能的捕获和转化(Wagner R,Dietzel L,K,et al.The long-term response to fluctuating light quality is animportant and distinct light acclimation mechanism that supports survival ofArabidopsis thaliana under low light conditions[J].Planta.2008,228(4):573-587.;Pfannschmidt T.Plastidial retrograde signalling--a true"plastid factor"or just metabolite signa tures?[J].Trends Plant Sci.2010,15(8):427-435.)。
综上所述,研究植物光合作用的调控机制,并通过遗传工程的方法来提高光合效率,对于我国农业现代化具有重要意义。
发明内容
本发明旨在至少在一定程度上解决现有技术中存在的技术问题之一,为此,本发明提供一种基因TL7,其是一类在植物中高度保守的基因,包括了植物所用物种的同源TL7基因,所述TL7基因的氨基酸序列与SEQ ID NO:1所示序列相似度大于70%,优选地,所述TL7基因的氨基酸序列与SEQ ID NO:2所示序列相似度大于70%。
本发明还提供一种基因TL7在水稻中的同源基因OsTL7的编码蛋白,其氨基酸序列如SEQ ID NO:1所示。
本发明还提供一种基因TL7在拟南芥中的同源基因AtTL7的编码蛋白,其氨基酸序列如SEQ ID NO:2所示。
编码氨基酸序列如SEQ ID NO:1所示蛋白的OsTL7基因,其核苷酸序列如SEQ IDNO:3所示。
编码氨基酸序列如SEQ ID NO:2所示蛋白的AtTL7基因,其核苷酸序列如SEQ IDNO:4所示。
本发明提供一种SEQ ID NO:1或SEQ ID NO:2所示的氨基酸序列或与SEQ ID NO:1或SEQ ID NO:2所示的氨基酸序列相似度大于70%的氨基酸序列在调控植物的生长发育以及光合作用效率中的应用。
本发明提供一种SEQ ID NO:3或SEQ ID NO:4所示的核苷酸序列或与SEQ ID NO:3或SEQ ID NO:4所示的核苷酸序列相似度大于70%的核苷酸序列在调控植物的生长发育以及光合作用效率中的应用。
本发明提供一种与SEQ ID NO:1所示的氨基酸序列或与SEQ ID NO:3所示的核苷酸序列在调控水稻的生长发育以及光合作用效率的应用。
本发明提供一种与SEQ ID NO:2所示的氨基酸序列或与SEQ ID NO:4所示的核苷酸序列在调控拟南芥的生长发育以及光合作用效率的应用。
本发明还提供一种上述基因TL7在植物光合调控中的应用,所述调控包括将基因TL7转入目的植物,提高基因TL7编码蛋白的表达或者活性,从而提高植物的生长发育以及光合作用的效率。
将基因TL7转入目的植物的技术包括但不限于转基因技术、杂交技术。
本发明还提供一种上述基因TL7在植物光合调控中的应用,所述调控包括将基因TL7经取代/添加/缺失一个或多个核苷酸而产生的突变体/等位基因/衍生物转入到目的植物,降低基因TL7编码蛋白的表达或者活性,从而延缓植物的生长发育以及光合作用的效率。
在本发明的技术方案中,基因TL7在植物光合调控中的应用,所述目的植物为单子叶植物、双子叶植物。
本发明还提供一种培育转基因植物的方法,包括:将TL7基因导入野生型植物,得到转基因植物,所述植物生长发育状况优于所述野生型植物或光合作用效率高于所述野生型植物;所述TL7基因的氨基酸序列与SEQ ID NO:1所示序列相似度大于70%,优选地,所述TL7基因的氨基酸序列与SEQ ID NO:2所示序列相似度大于70%。
本发明还提供一种培育转基因植物的方法,所述转基因植物为转基因水稻,培育转基因水稻的方法包括:将TL7同源基因OsTL7导入野生型水稻,得到转基因水稻,所述转基因水稻生长发育状况优于所述野生型水稻或光合作用效率高于所述野生型水稻;所述OsTL7基因的核苷酸序列如SEQ ID NO:3所示。
本发明还提供一种培育转基因植物的方法,所述转基因植物为转基因拟南芥,培育转基因拟南芥的方法包括:将TL7同源基因AtTL7导入野生型拟南芥,得到转基因拟南芥,所述转基因拟南芥生长发育状况优于所述野生型拟南芥或光合作用效率高于所述野生型拟南芥;所述AtTL7基因的核苷酸序列如SEQ ID NO:4所示。
本发明还提供一种含有核苷酸序列如SEQ ID NO:3所示基因的质粒。
本发明还提供一种含有核苷酸序列如SEQ ID NO:4所示基因的质粒。
本发明还提供一种含有核苷酸序列如SEQ ID NO:3所示基因的植物表达载体。
本发明还提供一种含有核苷酸序列如SEQ ID NO:4所示基因的植物表达载体。
本发明还提供一种宿主细胞,该宿主细胞含有核苷酸序列如SEQ ID NO:3或SEQID NO:4所示的基因序列。
上述宿主细胞,为大肠杆菌细胞、农杆菌细胞或者植物细胞。
本发明提供一种包含植物基因TL7的超表达突变体和超表达植株在制备转基因植物、农作物改良育种、制种中的应用。
本发明的有益效果在于:
1、本发明通过提高植物光合作用效率以培育高产植物品种;
2、本发明在自然光生长条件下改良农作物农艺性状增加农作物产量,具有广阔的应用空间以及市场前景。
附图说明
图1为OsTL7基因的CRISPR Cas9载体构建图;
图2为OsTL7基因的超表达载体构建图和相对表达量图,其中,图2A图为获得超表达OsTL7植株所构建的超表达载体,图2B图是野生型及超表达OsTL7植株的基因OsTL7相对表达量;
图3为野生型及突变体ostl7植株的大田表型图;
图4为野生型及超表达OsTL7植株的大田表型图,其中,图4A图是野生型及超表达OsTL7-1植株的大田表型,图4B图是野生型及超表达OsTL7-2植株的大田表型图;
图5为大田环境下野生型及突变体ostl7植株的株高和生物量干重统计图;
图6为大田环境下野生型及超表达OsTL7植株的株高和生物量干重统计图;
图7为T-DNA在拟南芥attl7突变体基因组DNA的插入位置图和相对表达量图,其中,图7A是拟南芥突变体attl7中T-DNA插入模式图,图7B是野生型及拟南芥突变体attl7中基因AtTL7的相对表达量;
图8为野生型及拟南芥attl7突变体植株的表型图;
图9为TL7的进化树图;
图10为TL7及TL7同源蛋白的氨基酸序列比对图。
具体实施方式
下面将结合实施例对本发明的方案进行解释。本领域技术人员将会理解,下面的实施例仅用于说明本发明,而不应视为限定本发明的范围。实施例中未注明具体技术或条件的,按照本领域内的文献所描述的技术或条件或者按照产品说明书进行。
实施例1:水稻敲除突变体ostl7的获得
为了研究水稻叶绿体定位基因的功能,我们运用CRISPR Cas9技术构建了大量水稻基因的突变体(Xie K,Minkenberg B,Yang Y.Boosting CRISPR/Cas9 multiplexediting capability with the endogenous tRNA-processing system[J].Proc NatlAcad Sci U S A,2015,112(11):3570-3575.DOI:10.1073/pnas.1420294112.)。其中一功能未知基因的突变体黄化矮小的表型引起了我们的关注。该基因突变体的T0代转化植株经PCR测序检测,筛选出纯合编辑的ostl7突变体材料,我们分别命名为ostl7-1以及ostl7-2。其中突变体ostl7-1材料编辑类型为第404碱基后插入C,其蛋白在第135氨基酸后发生移码突变;突变体ostl7-2材料编辑类型为第404碱基缺失T,其蛋白在第135氨基酸处的亮氨酸变成脯氨酸后发生提前终止。CRISPR Cas9创建突变体的载体为pRGEB32(https://www.addgene.org/63142/),在水稻OsTL7基因的外显子上设计2个靶位点来敲除OsTL7基因,靶位点序列为:
靶位点1:CCGTGGGCACGCGAGGGCGG;
靶位点2:CCCTGTACGCGCCGCTCCTA;
潮霉素基因鉴定引物为
F:CTCCATACAAGCCAACCACG;
R:GGAAGTGCTTGACATTGGGG。
Cas9基因鉴定引物为
F:GAGAATGCTGGCCTCTGCC;
R:GCTCTCTGATGGGCTTATCCCG。
CRISPR编辑检测测序引物为
F:TGGATTCGTTGCCCAAGTTG;
R:CCTTCCCGATCACGTCCTTCGTCTC。
OsTL7基因的CRISPR Cas9载体构建图如图1所示。
实施例2:超表达OsTL7水稻材料的获得
为了获得水稻OsSTL7超表达的材料,我们构建了水稻OsTL7基因的超表达载体。我们以水稻日本晴(Nipponbare)的cDNA作为模板,克隆OsTL7基因的CDS序列,并将其插入P1300载体的UBI10启动子后。通过农杆菌介导的方法将超表达载体转入水稻日本晴(Nipponbare)。获得的T0代转化植株经qPCR检测OsTL7基因的相对表达量,筛选出两个超表达OsTL7水稻材料。我们分别命名为超表达OsTL7-1以及超表达OsTL7-2,其中OsTL7表达量分别为野生型中OsTL7表达量的9.08和23.01倍。
克隆OsTL7基因CDS序列的引物为
F:GGGGACAAGTTTGTACAAAAAAGCAGGCTCCACCCGCGTCCTATCCATT;
R:GGGGACCACTTTGTACAAGAAAGCTGGGTACATGAGCAGCAGTGCAAAACTG。
检测OsTL7基因表达量的qPCR引物为
F:TGTACGCGCCGCTCCTA;
R:GGCCTTGAACCACAAGAATCC。
OsTL7基因的氨基酸序列如SEQ ID NO:1所示。
OsTL7基因的核苷酸序列如SEQ ID NO:3所示。
OsTL7基因的超表达载体构建图和相对表达量图如图2所示。其中,图2A是为得到超表达OsTL7植株所构建的超表达载体。图2B是野生型及两个超表达OsTL7的水稻材料(超表达OsTL7-1植株以及超表达OsTL7-2植株)的基因OsTL7相对表达量。由图2B可以看出,超表达OsTL7-1以及超表达OsTL7-2植株中OsTL7基因表达量分别为野生型中OsTL7基因表达量的9.08和23.01倍。。
实施例3:水稻野生型和突变体ostl7以及超表达OsTL7植株的大田表型
为了观察水稻野生型以及和突变体ostl7以及超表达OsTL7植株在大田生长条件下的表型,我们在2017年至2019年6月至10月在武汉(中国中部,北亚热带季风性湿润气候),取每种材料种植20株进行大田试验,使用至少T2代的纯合子种子。肥料的施用、杀虫剂的使用和所有其他田间管理措施都以同样的方式进行。
上述试验得到的野生型及突变体ostl7植株的大田表型图如图3所示。由图3可知,在大田生长条件下,水稻突变体ostl7的株高显著低于野生型,且水稻突变体ostl7分蘖数减少,叶片黄化。
上述试验得到的野生型及两个超表达OsTL7水稻材料(超表达OsTL7-1以及超表达OsTL7-2)植株的大田表型图如图4所示,其中,图4A是野生型及超表达OsTL7-1植株的大田表型。图4B图是野生型及超表达OsTL7-2植株的大田表型。由图4可知,在大田生长条件下,超表达OsTL7植株的株高显著高于野生型。
实施例4:株高和生物量的统计
我们将突变体ostl7和超表达OsTL7的T2代水稻材料以及野生型种植在武汉试验田,每份种植三个重复,每个重复各种植20株,进行株高、单株生物质干重的测定。每个重复取10株成熟期水稻单株,测量其地上部分的高度。生物量测定为每个重复取10株成熟期水稻单株,将其地上部分在阳光下完全晾干,然后用电子天平称取其重量。
上述数据均用T-test进行差异显著性比较分析。
其中,大田环境下野生型及突变体ostl7植株的株高和生物量干重统计图如图5所示,大田环境下野生型及超表达OsTL7植株的株高和生物量干重统计图如图6所示。
由图5可知,大田环境下野生型的株高显著高于突变体ostl7-1和突变体ostl7-2,并且大田环境下野生型的生物量干重显著大于突变体ostl7-1和突变体ostl7-2。
由图6可知,大田环境下超表达OsTL7-1的植株和超表达OsTL7-2的植株的株高均显著高于野生型,并且,大田环境下超表达OsTL7-1的植株和超表达OsTL7-2的植株的生物量干重均显著大于野生型。
实施例5:拟南芥AtTL7基因的分离克隆
为了研究拟南芥attl7敲除突变体的表型,我们在TAIR网站(https://www.arabidopsis.org/servlets/TairObject?type=germplasm&id=4650621)购买了T-DNA插入突变体(SALK_048033)。PCR检测结果表明该突变体的插入位置在AtTL7基因(TAIR编号:At3g51510)的第一个外显子中。T-DNA插入模式如图7A所示,中间标示区域为CDS区域,左右端为5’UTR与3’UTR区域。
检测attl7突变体的T-DNA插入位置引物为
F:GGATTAGATATTTCTATTCA;
R:TTGTTGATCTGAATATCAAC;
LB1.3:ATTTTGCCGATTTCGGAAC。
野生型及拟南芥attl7突变体的基因AtTL7相对表达量如图7B所示。
AtTL7基因的氨基酸序列如SEQ ID NO:2所示。
AtTL7基因的核苷酸序列如SEQ ID NO:4所示。
实施例6:拟南芥野生型及拟南芥attl7突变体植株的表型
为了观察拟南芥野生型(Col-0)以及突变体attl7的表型,每种材料取20颗种子,在含1%蔗糖的1/2MS培养基上,4℃低温诱导两天后再转入温室中培养5天,其生长条件(22℃,光照16h,黑暗8h),然后将小苗挪到营养土中生长14天,其生长条件不变。拟南芥野生型及拟南芥attl7突变体植株的表型如图8所示,突变体attl7的叶片呈黄绿色,且生长发育极为缓慢。
实施例7:TL7进化树的构建和氨基酸序列的比对
为了对植物TL7的同源基因进行系统发育分析,我们在PLAZA 4.5网站(https://bioinformatics.psb.ugent.be/plaza/versions/plaza_v4_5_monocots/)中,搜索了水稻OsTL7的同源蛋白序列(Van Bel M,Proost S,Wischnitzki E,et al.Dissecting plantgenomes with the PLAZA comparative genomics platform[J].Plant Physiol,2012,158(2):590-600.DOI:10.1104/pp.111.189514.)。其中选取了8个物种,分别为水稻、拟南芥、小麦、小米、大麦、玉米、高粱、番茄。我们用MEGA 7构建系统发育树,并基于最大似然法,使用1000次bootstrap重复构建(Kumar S,Stecher G,Li M,et al.MEGA X:MolecularEvolutionary Genetics Analysis across Computing Platforms[J].Mol Biol Evol,2018,35(6):1547-1549.DOI:10.1093/molbev/msy096.)。TL7的进化树图如图9所示。
用CLUSTAL X程序对该8个同源蛋白的氨基酸序列进行比对。TL7同源蛋白的氨基酸序列比较图如图10所示。其中,黑色阴影表示相同的氨基酸;灰色阴影的表示相似的氨基酸。TL7氨基酸序列的比对图如图10所示。
尽管上面已经示出和描述了本发明的实施例,可以理解的是,上述实施例是示例性的,不能理解为对本发明的限制,本领域的普通技术人员在本发明的范围内可以对上述实施例进行变化、修改、替换和变型。
序列表
<110> 武汉大学
<120> 植物光合调控基因TL7及其蛋白与应用
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 172
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 1
Met Ala Ala Ala Val Ser Ala Cys Ser Ser Arg Cys Leu Arg Leu Ile
1 5 10 15
Leu Gly Arg Pro Pro Arg Ala Arg Leu Ala Ser Val Val Glu Val Val
20 25 30
Glu Lys Arg Arg Arg Gly Gly Leu Val Ala Val Ala Ala Thr Glu Gly
35 40 45
Ser Ala Lys Ser Ser Gly Glu Ala Asp Glu Gln Val Pro Ser Trp Ala
50 55 60
Arg Pro Gly Ser Asp Glu Pro Pro Pro Trp Ala Arg Glu Gly Gly Gly
65 70 75 80
Gly Gly Gly Gln Gln Glu Pro Gly Ala Val Glu Leu Pro Phe Phe Ala
85 90 95
Tyr Leu Leu Ala Ser Ala Ile Thr Ala Ile Ala Ala Ile Gly Ser Ile
100 105 110
Phe Glu Tyr Ala Asn Gln Arg Pro Val Phe Gly Val Val Ser Pro Asp
115 120 125
Ser Ala Leu Tyr Ala Pro Leu Leu Gly Phe Phe Val Phe Thr Gly Ile
130 135 140
Pro Thr Ser Gly Phe Leu Trp Phe Lys Ala Val Gln Thr Ala Asn Lys
145 150 155 160
Glu Ala Glu Glu Gln Asp Arg Arg Asp Gly Phe Ser
165 170
<210> 2
<211> 181
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 2
Met Ala Ala Ser Leu Thr Ser Leu Pro Thr Gly Phe Cys Leu Ser His
1 5 10 15
Gly Asp Glu Cys Cys Asn Arg Ser Pro Thr Lys Ser Pro Phe Pro Gly
20 25 30
His His Pro Leu Ala Gly Arg Arg Lys Gly His Leu Leu His Tyr Glu
35 40 45
Arg Ser Thr Val Arg Arg Leu Val Val Thr Ala Ala Thr Glu Gly Ser
50 55 60
Lys Lys Ser Lys Glu Ser Glu Pro Ser Trp Ala Asn Pro Asp Ser Asp
65 70 75 80
Glu Pro Pro Pro Trp Ala Arg Asn Glu Gly Arg Ser Ser Thr Ser Gln
85 90 95
Glu Ser Phe Glu Val Pro Phe Phe Val Tyr Leu Leu Ala Ser Ala Ile
100 105 110
Thr Ala Ile Ala Ala Ile Gly Ser Val Phe Glu Tyr Thr Ser Lys Asn
115 120 125
Pro Val Phe Gly Ile Leu Glu Ser Asp Ser Ile Phe Tyr Thr Pro Val
130 135 140
Leu Gly Phe Phe Ala Leu Thr Gly Ile Pro Thr Ser Val Phe Leu Trp
145 150 155 160
Phe Lys Ser Val Glu Ala Ala Asn Lys Glu Ala Gln Glu Gln Asp Lys
165 170 175
Arg Asp Gly Phe Leu
180
<210> 3
<211> 1348
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
gcacgccacc cgcgtcctat ccattccacg gttcgtttcc accgcgcgcg ccacggcgag 60
ctcgccaacc cagccagcca tggcggcggc cgtgtccgcg tgcagctcgc ggtgcctccg 120
cctgatcctg gggcgccctc ctcgagcccg cctcgcgtcg gttgtggagg tcgtcgagaa 180
gaggaggagg ggcggactgg tggccgtggc ggcgacggag gggtcggcga agtcgtcggg 240
ggaggcggac gagcaggtcc cctcgtgggc gcgcccgggc tccgacgagc cgccgccgtg 300
ggcacgcgag ggcggcggcg gcgggggcca gcaggagccc ggcgccgtcg agctcccctt 360
cttcgcctac cttctcgcct ccgccatcac cgccatcgcc gccgtacgcc tctccacccc 420
ttcaactcct ctcctcctcc tcctcctctc gaatcccaaa ccctaaccat ggcgcgcaga 480
tcgggtccat cttcgagtac gcgaaccagc ggccggtgtt cggcgtggtg agccccgaca 540
gcgccctgta cgcgccgctc ctagggttct tcgtcttcac cgggataccc acctccgtac 600
gcatcctctc attgccaatc tccattgcga ggacatgaat ccaaattttc catggatttt 660
gattgcttgt tttaaaattt ttttgcaggg attcttgtgg ttcaaggccg tgcagacggc 720
gaacaaggag gcggaggagc aggatcgccg ggacggcttc tcgtgactga ttcagcatat 780
gaacatacgc atttgcagag taattaaagt ttgtgaatta ctgcttgtga ttcgtggtgt 840
taattaagtt ggtgacgctg aagaggggag acggatctgc gtattgacgc aatgaggaga 900
tgaattagca aggaagaaat ggcgatgttc aattgtacat caccgaaggg agctagctgt 960
tgaagtgaaa gtgtaaactc tgccttgtag ttgagaaata ttgagtgttt cgagcgagta 1020
taattacagc gaattatctg aacactgaat tatcgccacg gattatttgg tccatatgca 1080
tctgagagaa actgttttct tagacccgct attctaatct actttctcta tttcacaatg 1140
taagtcattc tagcatttcc cacattcgta ttgatgttaa tgaatctaga catatacatc 1200
tatctagatt cattaacatc aatatgaacg tgaaaaatgc tagaataact tatattgcga 1260
aatgaaggaa gtatctgtta gtactaagtt tgtaatttat actcccaccg tctcaaaata 1320
aatgcagttt tgcactgctc atgtataa 1348
<210> 4
<211> 1383
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
acagaatgat gatggattag atatttctat tcaaaaacta taacgtgtgg ctgcaaatcg 60
attcaccgct tcagactctg ttttagacca aagtcgagtg agtgctttca tcttcttctt 120
aagcatctat ggccgcgtct ctaacatctc ttccgaccgg tttttgtctt agccatggtg 180
atgaatgttg caaccgttca cctaccaaat caccatttcc aggccatcat cctctggctg 240
ggaggaggaa gggtcacttg ctccattacg aacgtagtac agtgaggaga ttggttgtga 300
cggcggcgac ggagggatct aaaaaatcta aagaaagtga accgtcttgg gcgaatcctg 360
actcagatga gccacctcct tgggctagaa acgaaggtcg ttcttctacg tcccaagaga 420
gctttgaggt tcctttcttt gtttatctgc tagcttccgc gattactgcc attgctgctg 480
tatgcttctt cttctctctc tttcactcct tttgttgttt gcattgctga ttctcatatc 540
aaagatgtta tcttaattat gcatcgtttg gtcactgtag attggttctg ttttcgagta 600
cacaagcaag aatccagttt tcgggatctt ggaatctgac agcatctttt atactcctgt 660
gcttggattc tttgctctta ctggaatccc cacttctgtg agttccttgt ctctctcttt 720
tcgtttttat ccatttgatc tgtatcagct gaaaaaatgg tttttttgcg ttcaggtgtt 780
cctatggttc aaatccgttg aagctgctaa taaggaagct caagaacaag ataaaagaga 840
tggctttctt taaaatcctt ttgtgtgatt ctgcaactca tacagtgatg attgttgtct 900
ccacatatac tttgaatata attctgcatt tacaaaggag cttgcttctg ttcactgttt 960
atcacccgct agattttgtt tgtaatttca acaatatgcc atgttccaac gagtaagtgc 1020
ctgaaattcg atgatcagct gtcctctctt ttatttgccc ctgtgaaaga tatgaatcca 1080
caaagaattc gatatgccat cctgcggttt gaatgtctga agctgatggt aatgtcacca 1140
tttgttcaat gagttgatat tcagatcaac aatttgttct ggaaagacat tgcctgcttt 1200
catttgttgg taggagattg gagtcaaaat atcagttgaa agttaattcc ttggcgcttg 1260
aagattgtgc ttgagcagag gctgtttccg gggaatgatc acatacaaaa tcatgtgttt 1320
aattggtttg tctatgaatg tattttcagt tgaatataag tcgagctcta ggctcaattt 1380
agc 1383
Claims (5)
1.SEQ ID NO:1或SEQ ID NO:2所示的氨基酸序列所示的多肽在调控植物的生长发育以及光合作用效率的应用,所述植物为水稻或拟南芥。
2.SEQ ID NO:3或SEQ ID NO:4所示的核苷酸序列所示的核酸在调控植物的生长发育以及光合作用效率的应用,所述植物为水稻或拟南芥。
3.根据权利要求1所述的应用,其特征在于,进一步包括培育转基因植物,培育转基因植物的方法,包括:将TL7基因导入野生型植物,得到转基因植物,所述植物生长发育状况优于所述野生型植物或光合作用效率高于所述野生型植物;所述TL7基因的氨基酸序列如SEQ ID NO:1所示,或所述TL7基因的氨基酸序列如SEQ ID NO:2所示,所述植物为水稻或拟南芥。
4.根据权利要求1所述的应用,其特征在于,进一步包括培育转基因植物,所述转基因植物为转基因水稻,培育转基因水稻的方法包括:将TL7同源基因OsTL7导入野生型水稻,得到转基因水稻,所述转基因水稻生长发育状况优于所述野生型水稻或光合作用效率高于所述野生型水稻;所述OsTL7基因的核苷酸序列如SEQ ID NO:3所示。
5.根据权利要求1所述的应用,其特征在于,进一步包括培育转基因植物,所述转基因植物为转基因拟南芥,培育转基因拟南芥的方法包括:将TL7同源基因AtTL7导入野生型拟南芥,得到转基因拟南芥,所述转基因拟南芥生长发育状况优于所述野生型拟南芥或光合作用效率高于野生型拟南芥;所述AtTL7基因的核苷酸序列如SEQ ID NO:4所示。
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