CN114480134B - Microalgae nutrition restoration liquid for relieving grassland desertification - Google Patents
Microalgae nutrition restoration liquid for relieving grassland desertification Download PDFInfo
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Abstract
The invention discloses a microalgae nutrition restoration liquid for relieving grassland desertification, which comprises a microalgae mixed liquid, wherein the microalgae mixed liquid comprises seven active microalgae, and the added microalgae comprises, by weight, 5-40 parts of Tarstaenia tenacissima, 5-40 parts of Sphaerotheca baumannii, 10-50 parts of Sphaerotheca schneideriana, 5-40 parts of pseudocladi, 10-50 parts of small Shan Qizao and 30-80 parts of chlorella pyrenoidosa, and further 10-50 parts of anabaena or 20 parts of gossypii are added. Aiming at grassland desertification areas, the nutrient restoring liquid can restore soil, supplement natural nitrogen, activate soil trace elements, improve plant stress resistance and prevent plant diseases and insect pests, seven microalgae can have good compatibility, and the number of microalgae active cells in each 1mL of nutrient restoring liquid can reach 10 6 The preservation period of the nutrient repairing liquid reaches 18 months, the nutrient repairing liquid is obtained through a large number of experiments in the cultivation process, and the cultivation efficiency is high.
Description
Technical Field
The invention belongs to the technical field of desertification control, and particularly relates to a microalgae nutrition restoration liquid for relieving grassland desertification.
Background
At present, two thirds of countries and regions exist in the world, a large amount of land areas face desertification threats, living space and sustainable development of human beings are seriously influenced, and desertification problems become ecological environment problems of global concern. Land degradation in desertification areas, productivity reduction, surface wind erosion desertification, moving dunes to press and bury farmlands, roads and houses, ecologically fragile, relatively backward economic conditions and great control difficulty.
Improving vegetation environment is an important means for controlling desertification, such as afforestation, grass cultivation, etc. However, the desertification area is barren, so that the nutrient components provided for the vegetation are seriously lost, the vegetation survival rate is low, and the control effect is poor. The application of chemical fertilizers can improve the vegetation survival rate to a certain extent, however, the negative effect caused by the application of chemical fertilizers cannot be ignored: the raw materials for producing the chemical fertilizer contain Zn, cu, co, cr and other heavy metals, and the heavy metals are applied into the soil along with the chemical fertilizer, so that the heavy metals in the soil are enriched, and enter the plant body through plant absorption to influence plant growth; chemical fertilizers can reduce the quantity and activity of soil microorganisms, thereby reducing the transformation of the soil microorganisms on organic matters in the soil, the decomposition of minerals and the degradation of toxic substances; the long-term application of the chemical fertilizer can aggravate the acidification of soil, aggravate the hardening of soil, inhibit the development of plant root systems and seriously reduce the stress resistance of plants.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the microalgae nutrition restoration liquid for relieving grassland desertification, and the microalgae nutrition restoration liquid has the advantages of soil restoration, long storage period and high culture efficiency.
In order to achieve the above purpose, the present invention provides the following technical solutions: a microalgae nutritional restoration liquid for relieving grassland desertification. The microalgae mixed solution comprises seven active microalgae, wherein the added microalgae comprises, by weight, 5-40 parts of Taernian silk algae, 5-40 parts of Sphingomonas baumannii, 10-50 parts of Sphingomonas vaginalis, 5-40 parts of pseudo-cladophora, 10-50 parts of small Shan Qizao and 30-80 parts of chlorella pyrenoidosa, and alternatively 10-50 parts of anabaena or 20 parts of gossypium.
Preferably, the microalgae mixed solution prepared by mixing the seven active algae solutions in the second step according to the weight parts comprises 10 parts of Taernian, 10 parts of Sphaerotheca pallidum, 10 parts of Sphaerotheca fuliginea, 5 parts of pseudo-cladophora, 5 parts of small Shan Qi algae, 10 parts of nitrogen fixation anabaena and 30 parts of chlorella pyrenoidosa.
Preferably, the microalgae mixed solution prepared by mixing the seven active algae solutions in the second step according to the weight parts comprises 30 parts of Taernian, 20 parts of Sphaerotheca pallidum, 30 parts of Sphaerotheca fuliginea, 20 parts of Pseudocladophora, 20 parts of Chlorella parvifolia, 30 parts of anabaena azotifolia and 50 parts of Chlorella pyrenoidosa.
Preferably, the microalgae mixed solution prepared by mixing the seven active algae solutions in the second step according to the weight parts comprises 50 parts of Taernian, 40 parts of Sphaerotheca pallidum, 50 parts of Sphaerotheca fuliginea, 40 parts of pseudo-cladophora, 40 parts of small Shan Qi algae, 50 parts of nitrogen fixation anabaena and 80 parts of chlorella pyrenoidosa.
Preferably, the microalgae mixed solution prepared by mixing the seven active algae solutions in the second step according to the weight parts comprises 10 parts of Taernian, 10 parts of Sphaerotheca pallidus, 20 parts of Sphaerotheca fuliginea, 10 parts of pseudo-cladophora, 10 parts of small Shan Qi algae, 20 parts of gossypium and 40 parts of Chlorella pyrenoidosa.
Preferably, the microalgae mixed solution prepared by mixing the seven active algae solutions in the second step according to the weight parts comprises 40 parts of Taernian, 30 parts of Sphaerotheca pallidum, 40 parts of Sphaerotheca fuliginea, 30 parts of Pseudocladophora, 30 parts of small Shan Qi algae, 40 parts of nitrogen fixation anabaena and 60 parts of Chlorella pyrenoidosa.
By adopting the scheme, the Taerdesert algae, the Sphaerotheca baumannii, the Microcystis vaginalis with the sheath, the nitrogen fixation anabaena, the small Shan Qizao and the pseudo-cladium belong to blue algae, wherein the nitrogen fixation anabaena, the small Shan Qizao and the pseudo-cladium have high-efficiency nitrogen fixation and decomposition functions, can fix molecular nitrogen in the atmosphere into combined nitrogen, further synthesize protein and increase soil or water fertility. After entering the soil, the blue algae cells can wrap the plant root system and provide sufficient nutrients for the root along with the extension of the root system. Chlorella pyrenoidosa belongs to green algae, has strong photosynthesis and remarkable solar energy intake, can perform photosynthesis in the state of visible light source, can increase dissolved oxygen in water body, and can degrade ammonia nitrogen and H in water body 2 S, nitrite, etc.
Seven microalgae including Taerdesert wire algae, sphaerotheca baumannii, sphaerotheca fuliginea, pseudocladophora, small Shan Qi algae, nitrogen fixation anabaena and chlorella pyrenoidosa can have good compatibility, and the number of active cells of the microalgae can reach 10 in each 1mL of nutrient repairing liquid 6 The preservation period of the nutrient repairing liquid reaches 18 months. The soil-based nutrient solution is added into nutrient repairing solution according to a reasonable proportion, and enters the soil: on one hand, microalgae in the nutrition restoration liquid can wrap plant roots, form a symbiotic interaction relationship with the plant roots, fix nitrogen from air, decompose and dissolve insoluble phosphorus and potassium, activate insoluble calcium, magnesium or sulfur elements to become an effective state which can be absorbed by plants, be absorbed by the plants by themselves and promote the root development of the plants; on the other hand, the microalgae in the nutrient repairing liquid can directly provide the nutrient substances for plants through the symbiotic relationship between microorganisms and plants, promote the absorption of plant root systems to the nutrient, and promote the growth of the plant root systemsOne step of promoting the development of plant root systems; furthermore, the microalgae in the nutrient repairing liquid can survive and propagate on the surface of plant root systems, can enter the inside of plants, survive in the roots, stems, leaves and fruits of the plants, and provide rich and balanced natural nutrients for the plants.
Compared with the existing chemical fertilizer, the nutrient repairing liquid disclosed by the invention does not contain any chemical components: the enrichment of heavy metal elements in the soil is not caused, the absorption of the heavy metal elements by plants is effectively reduced, and the quality of plant growth, agricultural products and the like is further improved; the method does not reduce the quantity and activity of soil microorganisms, and does not influence the transformation of the soil microorganisms on organic matters in the soil, the decomposition of minerals and the degradation of toxic substances; the phenomena of acidification, hardening and the like of the soil can not be caused.
In conclusion, the nutrient repairing liquid provided by the invention is used as a biological fertilizer with rich nutrition, has good compatibility, and can repair soil, supplement natural nitrogen, activate trace elements in soil, improve plant stress resistance and prevent plant diseases and insect pests.
Another object of the present invention is to provide a cultivation method of the microalgae nutrient repairing liquid for relieving grassland desertification, comprising the following steps:
step one: the seven active microalgae are selected or separated to be respectively cultivated in an enlarged way to obtain seven active microalgae liquid, and the seven active microalgae are cultivated in six different stages;
step two: mixing seven active algae solutions according to parts by weight to obtain microalgae mixed solution;
in the first step, nitrogen source, phosphorus source and inorganic salt solution required by microalgae proliferation are input in the process of expanding cultivation, and air and carbon dioxide gas are introduced to keep stable illumination and temperature conditions;
in the second step, nitrogen source, phosphorus source and inorganic salt solution required by microalgae proliferation are input in the mixing process of the algae liquid, air is continuously introduced, carbon dioxide gas is intermittently introduced, and stable illumination and temperature conditions are maintained;
wherein the illumination intensity in the first step and the second step is 2300-2700lux, and the temperature is 25-27 ℃.
The extended cultivation time of the seven active microalgae at each stage is 10-15 days.
Compared with the prior art, the invention has the following beneficial effects:
1. aiming at grassland desertification areas, the nutrient restoring liquid can restore soil, supplement natural nitrogen, activate soil trace elements, improve plant stress resistance and prevent plant diseases and insect pests.
2. In the nutrient repairing liquid, seven microalgae can have good compatibility, and the number of active cells of the microalgae in each 1mL nutrient repairing liquid can reach 10 6 The preservation period of the nutrient repairing liquid reaches 18 months.
3. The enlarged cultivation process is obtained through a large number of experiments, and the cultivation efficiency is high.
Detailed Description
All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides a technical scheme that: a microalgae nutritional restoration liquid for relieving grassland desertification.
Example 1
A cultivation method of microalgae nutrition restoration liquid for relieving grassland desertification comprises the following steps:
s1, respectively expanding and cultivating Talaromyces tartraria, sphingei with sheath, pseudocladosporium cucumerinum, xiao Shan Qi, anabaena azotifolia and Chlorella pyrenoidosa by purified water under the aseptic environment with the illumination intensity of 2300lux and the temperature of 25 ℃ to obtain seven algae solutions,
the expansion cultivation process of the seven algae liquids comprises the following steps: selection and isolation of monomer cells were cultured in 15mL tubes for 10 days, the number of microalgae-active cells was 10 6 Inoculating into 1OOmL flask, culturing for 10 days, and culturing to obtain microalgae active cell number of 10 6 Inoculating into 1L flask, culturing for 10 days, and culturing to obtain microalgae active cell number of 10 6 personal/mL-transferInoculating into 5L flask, culturing for 10 days to obtain microalgae active cell number of 10 6 Inoculating into 18L reaction barrel, culturing for 10 days, and culturing to obtain microalgae active cell number of 10 6 Inoculating into 800L reactor for 10 days, and culturing to obtain microalgae active cell with number of 10 6 And each mL.
In the process of expanding cultivation, inputting nitrogen source, phosphorus source and inorganic salt solution required by growth of microalgae active cells, continuously introducing air and intermittently introducing carbon dioxide gas.
S2, under the conditions of a sterile environment, illumination intensity of 23001ux, temperature of 25 ℃ and nitrogen source, phosphorus source, inorganic salt solution, air and carbon dioxide gas which are required by the growth of microalgae active cells, mixing seven kinds of algae liquid to obtain the microalgae nutrition restoration liquid, wherein the number of the microalgae active cells is 10 6 And (2) each mL, wherein the weight parts of the components comprise 10 parts of Talaromyces, 10 parts of Sphaerotheca pallidum, 10 parts of Sphaerotheca fuliginea, 5 parts of pseudo-cladophora, 5 parts of small Shan Qi algae, 10 parts of nitrogen-fixing anabaena and 30 parts of chlorella pyrenoidosa.
Example 2
A cultivation method of microalgae nutrition restoration liquid for relieving grassland desertification comprises the following steps:
s1, respectively expanding and cultivating Talaromyces gracilis, sphaerotheca pallidum, microcystis vaginalis, pseudocladosporium, chlorella vulgaris, anabaena azotifolia and Chlorella pyrenoidosa by using purified water under a sterile environment with the illumination intensity of 25001ux and the temperature of 26 ℃ to obtain seven algae solutions,
the expansion cultivation process of the seven algae liquids comprises the following steps: selection and isolation of monomer cells were cultured in 15mL tubes for 12 days, the number of microalgae-active cells was 10 6 The cells were inoculated into a 100mL flask for 12 days, and the number of microalgae active cells was 10 6 Inoculating into 1L flask, culturing for 12 days, and culturing to obtain microalgae active cell number of 10 6 Inoculating into 5L flask, culturing for 12 days, and culturing to obtain microalgae active cell number of 10 6 Inoculating into 18L reaction barrel, culturing for 12 days, and culturing to obtain microalgae active cell number of 10 6 Inoculating into 800L reactor for cultivationFor 12 days, the number of microalgae active cells is 10 6 And each mL.
In the process of expanding cultivation, inputting nitrogen source, phosphorus source and inorganic salt solution required by growth of microalgae active cells, continuously introducing air and intermittently introducing carbon dioxide gas.
S2, under the conditions of a sterile environment, an illumination intensity of 25001ux, a temperature of 26 ℃ and nitrogen source, phosphorus source, inorganic salt solution, air and carbon dioxide gas which are required by the growth of microalgae active cells, mixing seven kinds of algae liquid to obtain the microalgae nutrition restoration liquid, wherein the number of the microalgae active cells is 10 6 And the total weight of the feed additive is 30 parts of Tachyrhizus, 20 parts of Sphaerotheca baudiana, 30 parts of Sphaerotheca fuliginea, 20 parts of pseudo-cladophora, 20 parts of small Shan Qi algae, 30 parts of nitrogen-fixing anabaena and 50 parts of chlorella pyrenoidosa.
Example 3
A cultivation method of microalgae nutrition restoration liquid for relieving grassland desertification comprises the following steps:
s1, respectively expanding and cultivating Talaromyces gracilis, sphaerotheca pallidum, microcystis vaginalis, pseudocladosporium, chlorella vulgaris, anabaena azotifolia and Chlorella pyrenoidosa by adopting purified water under the aseptic environment with the illumination intensity of 27001ux and the temperature of 27 ℃ to obtain seven algae solutions,
the expansion cultivation process of the seven algae liquids comprises the following steps: selection and isolation of monomer cells were cultured in 15mL tubes for 15 days, the number of microalgae-active cells was 10 6 The cells were inoculated into a 100mL flask for 15 days, and the number of microalgae active cells was 10 6 Inoculating into 1L flask, culturing for 15 days, and culturing to obtain microalgae active cell number of 10 6 Inoculating into 5L flask, culturing for 15 days, and culturing to obtain microalgae active cell number of 10 6 Inoculating into 18L reaction barrel, culturing for 15 days, and culturing to obtain microalgae active cell with number of 10 6 Inoculating into 800L reactor for 15 days, and culturing to obtain microalgae active cell with number of 10 6 And each mL.
In the process of expanding cultivation, inputting nitrogen source, phosphorus source and inorganic salt solution required by growth of microalgae active cells, continuously introducing air and intermittently introducing carbon dioxide gas.
S2, mixing five algae solutions to obtain microalgae nutrition restoration solution under the conditions of sterile environment, illumination intensity of 27001ux, temperature of 27 ℃ and nitrogen source, phosphorus source, inorganic salt solution, air and carbon dioxide gas required by growth of microalgae active cells, wherein the number of the microalgae active cells is 10 6 And (2) each mL, wherein the weight parts of the components comprise 50 parts of Talaromyces, 40 parts of Sphaerotheca pallidum, 50 parts of Sphaerotheca fuliginea, 40 parts of pseudo-cladophora, 40 parts of small Shan Qi algae, 50 parts of nitrogen-fixing anabaena and 80 parts of chlorella pyrenoidosa.
Example 4
This example was conducted as in example 2, except that:
in the step 2, 10 parts of Taernian, 10 parts of Sphaerotheca baudiana, 20 parts of Sphaerotheca fuliginea, 10 parts of pseudo-cladophora, 10 parts of small Shan Qi algae, 20 parts of cotton algae and 40 parts of Chlorella pyrenoidosa are mixed to obtain the microalgae nutrition restoration liquid.
Example 5
This example was conducted as in example 2, except that:
in the step S2, 40 parts of Taernian, 30 parts of Sphaerotheca baudiana, 40 parts of Sphaerotheca fuliginea, 30 parts of pseudo-cladophora, 30 parts of small Shan Qi algae, 40 parts of nitrogen fixation anabaena and 60 parts of chlorella pyrenoidosa are mixed to obtain the microalgae nutrition restoration liquid.
Comparative example 1
Conventional chemical fertilizers.
Microalgae nutrition restoration liquid soil improvement test
The old wheat grass is a common plant for preventing and treating grassland desertification, 4 areas of the same planting field are selected in the test, the old wheat grass is planted, and the microalgae nutrition restoration liquid prepared in examples 1-3 and the conventional chemical fertilizer prepared in comparative example 1 are respectively used for fertilization, so that a comparative test for 3 years is carried out. During the period: soil of 0-20cm of old wheat mango root system area is taken, alkaline hydrolysis nitrogen of the soil is measured to reflect the nitrogen supply condition of the soil, and the detection result is shown in table 1.
TABLE 1 soil alkaline hydrolysis Nitrogen test results
As can be seen from Table 1, the microalgae nutrient repairing liquid prepared in examples 1-5 can obviously increase the content of alkaline hydrolysis nitrogen in soil on one hand, and can cause the increase of the alkaline hydrolysis nitrogen in the soil to be in a trend of increasing year by year on the other hand, and the effects of the microalgae nutrient repairing liquid are obviously better than those of the conventional chemical fertilizer of comparative example 1.
This mainly benefits from: on one hand, the microalgae nutrition restoration liquid has high-efficiency nitrogen fixation and decomposition functions, can fix molecular nitrogen in the atmosphere into combined nitrogen, further synthesizes protein, and increases soil or water fertility; on the other hand, the microalgae nutrient repairing liquid has good compatibility among various microalgae, and the number of microalgae active cells in each ImL nutrient repairing liquid can reach 10 6 The preservation period of the nutrient repairing liquid reaches 18 months, and nutrient substances can be injected into soil for a long time and high efficiency.
In addition, experiments show that the soil hardening phenomenon is obviously improved after the microalgae nutrient repairing liquid is applied, and further the microalgae nutrient repairing liquid has great significance in the aspects of increasing the content of organic matters in soil, reducing the volume weight, improving the soil hardening and the like, is beneficial to preventing and controlling the growth of vegetation in grassland desertification, and has strong practical significance for link grassland desertification.
The main root length of the old wheat middling was measured, and the difference between the main root length of the old wheat middling corresponding to each example and the main root length of the old wheat middling corresponding to the comparative example was calculated, and the calculation results are shown in table 2.
TABLE 2 Main root Length calculation results of old Miscanthus
As can be seen from table 2, the microalgae nutrient repairing liquid prepared in examples 1 to 5 can significantly increase the length of the main root of old wheat, compared with the conventional chemical fertilizer of comparative example 1, wherein the microalgae nutrient repairing liquid prepared in example 2 has the most significant effect.
This mainly benefits from: on one hand, microalgae in the nutrition restoration liquid can coexist and propagate on the surface of a plant root system and in the plant, form a symbiotic interaction relationship with the plant root system, fix nitrogen in the air, decompose and dissolve insoluble phosphorus and potassium, activate insoluble calcium, magnesium or sulfur elements, provide rich and balanced natural nutrients for the plant, promote the root system development of the plant and promote the absorption of nutrient substances by the plant; on the other hand, the microalgae nutrient repairing liquid has good compatibility among various microalgae, and the number of microalgae active cells in each 1mL nutrient repairing liquid can reach 10 6 The preservation period of the nutrient repairing liquid reaches 18 months, and the nutrient repairing liquid can provide nutrient substances for plants for a long time and high efficiency.
In addition, the microalgae nutrient repairing liquid does not contain any chemical component, can degrade toxic substances, does not reduce the quantity and activity of soil microorganisms, does not influence the transformation of the soil microorganisms on organic matters in the soil, the decomposition of minerals and the degradation of the toxic substances, and further promotes the growth of plants.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A microalgae nutrition restoration liquid for relieving grassland desertification is characterized in that: the microalgae consists of seven active microalgae, wherein the added microalgae comprise, by weight, 5-40 parts of Taernian silk algae, 5-40 parts of Sphaerotheca baumannii, 10-50 parts of Sphaerotheca schneideriana, 5-40 parts of pseudo-cladi, 10-50 parts of small Shan Qizao and 30-80 parts of chlorella pyrenoidosa, and alternatively 10-50 parts of anabaena or 20 parts of gossypium;
the preparation process of the microalgae nutrition restoration liquid comprises the following steps:
step one: the seven active microalgae are separated and cultivated in an enlarged way to obtain seven active microalgae liquid, and the seven active microalgae are cultivated in six different stages;
step two: mixing seven active microalgae according to parts by weight to obtain microalgae nutrition restoration liquid;
in the first step, nitrogen source, phosphorus source and inorganic salt solution required by microalgae proliferation are input in the process of expanding cultivation, and air and carbon dioxide gas are introduced to keep stable illumination and temperature conditions;
in the second step, the nitrogen source, the phosphorus source and the inorganic salt solution required by microalgae proliferation are input in the mixing process of the active microalgae, air is continuously introduced, carbon dioxide gas is intermittently introduced, and stable illumination and temperature conditions are maintained;
wherein the illumination intensity in the first step and the second step is 2300-2700lux, and the temperature is 25-27 ℃.
2. The microalgae nutrient repair liquid for relieving grassland desertification as claimed in claim 1, which is characterized in that: the extended cultivation time of seven active microalgae at each stage is 10-15 days.
3. The microalgae nutrient repair liquid for relieving grassland desertification as claimed in claim 1, which is characterized in that: the microalgae nutrition restoration liquid prepared by mixing seven active microalgae in the second step according to parts by weight is 30 parts of Tar desert silk algae, 20 parts of Sphingomonas baumannii, 30 parts of Sphingomonas vaginalis, 20 parts of pseudo-cladophora, 20 parts of small Shan Qi algae, 30 parts of nitrogen fixation anabaena and 50 parts of chlorella pyrenoidosa.
4. The microalgae nutrient repair liquid for relieving grassland desertification as claimed in claim 1, which is characterized in that: the microalgae nutrition restoration liquid prepared by mixing seven active microalgae in the second step according to the weight parts is 10 parts of Taernian, 10 parts of Sphingei bauhinia, 20 parts of Sphingei, 10 parts of pseudo-cladi, 10 parts of small Shan Qi algae, 20 parts of cotton algae and 40 parts of Chlorella pyrenoidosa.
5. The microalgae nutrient repair liquid for relieving grassland desertification as claimed in claim 1, which is characterized in that: the microalgae nutrition restoration liquid prepared by mixing seven active microalgae in the second step according to parts by weight is 40 parts of Tar desert silk algae, 30 parts of Sphingomonas baumannii, 40 parts of Sphingomonas vaginalis, 30 parts of pseudo-cladophora, 30 parts of small Shan Qi algae, 40 parts of nitrogen fixation anabaena and 60 parts of chlorella pyrenoidosa.
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