CN114988964B - Application of microalgae compound conditioner in soil improvement of urban relocation place - Google Patents
Application of microalgae compound conditioner in soil improvement of urban relocation place Download PDFInfo
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- CN114988964B CN114988964B CN202210776896.7A CN202210776896A CN114988964B CN 114988964 B CN114988964 B CN 114988964B CN 202210776896 A CN202210776896 A CN 202210776896A CN 114988964 B CN114988964 B CN 114988964B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The application relates to application of a microalgae compound conditioner in soil improvement of urban moving places, which comprises the following components in parts by weight:the combined microalgae liquid is formed by mixing nitrogen-fixing cyanobacteria and filamentous cyanobacteria, wherein the nitrogen-fixing cyanobacteria comprises anabaena, nostoc and bifidobacterium, and the filamentous cyanobacteria comprises Microcystis vaginalis, microcystis macroalgae and Sphaerotheca malanii; the biochar is prepared from urban greening waste, and the bio-organic fertilizer is a commercial product conforming to the industry standard NY/T525-2021; the application also relates to a preparation method and application of the microalgae compound conditioner. The microalgae compound conditioner can effectively overcome the barriers of low biological activity and the like caused by barren soil nutrients and lack of energy substances of heterotrophic microorganisms in the moving place, effectively improve the soil quality, reasonably treat urban solid wastes, reduce the repair cost and do not need subsequent maintenance.
Description
Technical Field
The application relates to the technical field of soil improvement, in particular to a microalgae compound conditioner for improving soil of urban moving places, a preparation method and application thereof.
Background
The urban greening area and quality are important indexes for measuring urban ecological civilization construction. However, with the increasing population of cities, the rapid increase of urban greening demands is increasingly contradictory with the shortage of land resources. The displacement and development of urban village and industrial land have become an important means for sustainable utilization of urban land. The movable land such as village, industrial and enterprise land and the like in cities is used for greening construction, and has become a main source for the growth of green areas in large and medium cities. However, the soil of the urban relocation place is generally poor in soil, the soil structure is chaotic, and the soil has low biological activity, so that the soil is a difficult point for quick greening of the urban relocation place.
The Chinese patent of application No. CN 108517213A discloses a soil conditioner for improving the drought resistance and the water and fertilizer utilization efficiency of soybeans by utilizing microalgae fermentation broth, but the patent mainly focuses on improving the physiological stress resistance of the soybeans by the conditioner. The Chinese patent of application number CN 113578943A shows that the microalgae can effectively repair the heavy metal pollution of soil. However, none of these patents effectively utilizes the autotrophic characteristics of microalgae to screen for microalgae suitable for improvement by uncured raw soil, and to develop more efficient soil conditioners. Aiming at the obstacle factors such as poor soil nutrient and high soil pH of the urban relocation land, a soil conditioner capable of effectively improving soil fertility of the urban relocation land and improving restoration efficiency is urgently needed.
Disclosure of Invention
The application aims to improve urban relocation soil from underground deep layers and uncured cities, and particularly provides a microalgae compound conditioner for improving urban relocation soil, a preparation method and application thereof, aiming at the problems of urban relocation soil nutrient deficiency and overhigh pH. The microalgae compound conditioner, the preparation method and the application thereof can realize targeted modification, effectively improve soil fertility of urban relocation sites and improve repair efficiency.
In order to achieve the above purpose, the application adopts the following technical scheme:
the microalgae compound conditioner for improving the soil of the urban moving land comprises the following components in parts by weight:
20-25 parts of combined microalgae liquid
20-25 parts of biochar
50-60 parts of biological organic fertilizer
Wherein, the liquid crystal display device comprises a liquid crystal display device,
the combined microalgae liquid is formed by mixing nitrogen-fixing blue algae and filamentous blue algae, wherein the nitrogen-fixing blue algae comprises anabaena, nostoc and bifidobacterium, and the filamentous blue algae comprises microcystis vaginalis, microcystis macroalgae and sphingale wari;
the biochar is prepared from urban greening waste, and the bio-organic fertilizer is a commercial product conforming to the industry standard NY/T525-2021.
According to the microalgae compound conditioner for improving the soil of the urban relocation place, as an optimal design scheme, the mixing ratio of nitrogen-fixing blue algae and filamentous blue algae in the combined microalgae liquid is 3:2. The application relates to a microalgae compound conditioner for improving soil in urban moving places, which is used as an optimal design scheme, wherein the nitrogen-fixing blue algae comprises the following components in percentage by weight: 1:1.
the application relates to a microalgae compound conditioner for improving soil in urban relocation places, which is used as an optimal design scheme, wherein the filamentous cyanobacteria comprises the components of Sphaerotheca fuliginea, sphaerotheca malanii and Sphaerotheca malanii in a proportion of 1:1:1.
according to the microalgae composite conditioner for improving the soil of the urban relocation place, as an optimal design scheme, the preparation method of the biochar comprises the steps of placing the dried urban greening waste into a microwave muffle furnace for pyrolysis, introducing nitrogen at the flow rate of 250L/h before heating, then heating to 450 ℃ at the speed of 5 ℃/min, and maintaining the temperature for 1 hour; and naturally cooling to 100 ℃, and stopping introducing nitrogen to obtain the prepared biochar.
The microalgae compound conditioner for improving the soil of urban moving places has the performance index that the organic matters are more than or equal to 45 percent, the effective viable count is more than or equal to 2 hundred million/g and N+P is adopted as an optimal design scheme 2 O 5 +K 2 O≥5％。
The application also relates to a preparation method of the microalgae compound conditioner for improving the soil of the urban relocation place, which comprises the following steps:
firstly, preparing biochar particles: the biochar is subjected to sieving treatment, and the particle diameter of the biochar is required to be less than or equal to 1cm.
Secondly, preparing a microalgae-biochar compound: and (3) putting the prepared biochar particles into 0.75g/L of the combined microalgae solution which is cultured in advance, and stirring at the room temperature for 24 hours at the rotating speed of 250rpm to obtain the microalgae-biochar compound.
Thirdly, preparing the microalgae-biochar-bio-organic fertilizer conditioner: mixing the microalgae-biochar compound according to a required proportion to obtain the required conditioner.
As an optimal design scheme of the preparation method of the microalgae compound conditioner for improving the soil of the urban moving land, in the first step, the unscreened biochar particles need to be crushed again, and then are screened after being crushed.
As an optimal design scheme of the preparation method of the microalgae compound conditioner for improving urban moving land soil, in the third step, the mixing weight ratio of the microalgae-biochar compound to the bio-organic fertilizer is 1:3.
the application also provides application of the microalgae compound conditioner in improving the soil of the urban relocation place, and the indexes before the soil restoration of the urban relocation place are as follows:
pH value 8.9 organic matter 5.1g/kg total salt 2.3g/kg alkaline hydrolysis nitrogen 8.5mg/kg
58.9mg/kg of quick-acting phosphorus and 29.4mg/kg of quick-acting potassium,
the application amount of the microalgae compound conditioner in the urban relocation soil is 8%, and the physicochemical indexes of the urban relocation soil after two months of application are as follows:
pH value is 6.8, organic matter is 27.5g/kg total salt content is 0.8g/kg alkaline hydrolysis nitrogen is 52.3mg/kg quick-acting phosphorus is 96.4mg/kg quick-acting potassium is 79.4mg/kg.
For urban relocation soil, the indexes before restoration are as follows: the pH value of the organic matter is 8.9, the total salt content is 5.1g/kg, the alkaline hydrolysis nitrogen is 2.3g/kg, the quick-acting phosphorus is 8.5mg/kg, the quick-acting potassium is 58.9mg/kg, and the quick-acting potassium is 29.4mg/kg.
The application fully utilizes the influence of microalgae on soil quality. Specifically, the autotrophic characteristics and the extremely strong environment adaptability of the microalgae are fully utilized, and the microalgae species which are suitable for urban moving places and are not improved by cured raw soil are screened. In particular, heterotrophic microorganisms in traditional microbial soil conditioners are difficult to survive and perform the functions of urban relocation soil nutrient deficiency, high soil pH and low biological activity; through experiments, the mixed algae solution of the nitrogen-fixing blue algae and the filamentous blue algae can grow and reproduce well in urban moving soil, and secondary metabolites and dead residues can excite the growth of soil primary microorganisms, so that the biological activity of the soil is greatly improved.
The nitrogen-fixing blue algae can simultaneously perform photosynthesis and nitrogen fixation, can convert molecular nitrogen in air into a nitrogen compound, and secrete active substances such as amino acid, polysaccharide, polypeptide and the like in the growth process.
The silk-like blue algae ecological niche is wider, can grow and reproduce well in alkaline soil, has a thicker viscose layer outside cells of the silk-like blue algae, can absorb a large amount of water and form algae crust on the soil surface, thereby effectively preventing water and soil loss.
Fixation of CO by soil microalgae through photosynthesis 2 And active substances such as amino acid, sugar, polypeptide, hormone and the like are secreted in the growth and propagation process, so that the growth of other microorganisms can be promoted, and the soil fertility can be synergistically improved.
Compared with the prior art, the application has the following beneficial effects:
1. according to the application, urban greening waste is recycled, and the urban greening waste is prepared into biochar for land utilization, so that urban solid waste can be reasonably treated, and the soil quality can be effectively improved.
2. The application inoculates autotrophic soil microalgae capable of photosynthesis into the soil of the moving place, which can effectively overcome the defect that the soil of the moving place is barren, and the soil microorganisms lack energy and cannot survive.
3. The preparation cost of the microalgae compound conditioner can be well controlled, the repair cost of urban moving soil is low, the subsequent maintenance is not needed, the soil can be improved after the ground building is removed, and the soil can be improved through the growth and propagation process of the microalgae.
Drawings
FIG. 1 is a flow chart of a method for preparing a microalgae compound conditioner for improving soil in urban moving places.
Detailed Description
The present application will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the present application, but are not intended to limit the application in any way. It should be noted that variations and modifications could be made by those skilled in the art without departing from the inventive concept. These are all within the scope of the present application.
Soil microalgae are important components of soil microorganisms, play an important role in the processes of soil formation, succession, soil composition and the like, and are pioneers in desert zones. The application utilizes the microalgae compound conditioner for targeted restoration, thereby effectively improving the soil fertility of urban relocation and improving the restoration efficiency.
The application provides a microalgae compound conditioner for improving soil in urban moving places, which comprises the following components in parts by weight:
20-25 parts of combined microalgae liquid
20-25 parts of biochar
50-60 parts of biological organic fertilizer
Wherein, the liquid crystal display device comprises a liquid crystal display device,
the combined microalgae liquid is formed by mixing nitrogen-fixing blue algae and filamentous blue algae, wherein the nitrogen-fixing blue algae comprises anabaena, nostoc and bifidobacterium, and the filamentous blue algae comprises microcystis vaginalis, microcystis macroalgae and sphingale wari;
the biochar is prepared from urban greening waste, and the preparation method of the biochar comprises the steps of placing the dried urban greening waste into a microwave muffle furnace for pyrolysis, introducing nitrogen at the flow of 250L/h before heating, heating to 450 ℃ at the speed of 5 ℃/min, and maintaining the temperature for 1 hour; and naturally cooling to 100 ℃, and stopping introducing nitrogen to obtain the prepared biochar.
The bio-organic fertilizer is a commercial product conforming to the line standard NY/T525-2021.
The mixing ratio of the nitrogen-fixing blue algae and the filamentous blue algae in the combined microalgae liquid is 3:2. the nitrogen-fixing blue algae comprises the components of anabaena, nostoc and bifidobacterium in a proportion of 3:1:1. the filamentous cyanobacteria comprises the components of the microcystis vaginalis, the microcystis martensii and the Sphingomonas maladshurica in the proportion of 1:1:1.
the application fully utilizes the influence of microalgae on soil quality, the nitrogen-fixing blue algae can simultaneously carry out photosynthesis and nitrogen fixation, and the nitrogen-fixing blue algae can convert molecular nitrogen in air into nitrogen compounds and secrete active substances such as amino acids, polysaccharides, polypeptides and the like in the growth process. The silk-like blue algae ecological niche is wider, can grow and reproduce well in alkaline soil, has a thicker viscose layer outside cells of the silk-like blue algae, can absorb a large amount of water and form algae crust on the soil surface, thereby effectively preventing water and soil loss.
The specially prepared soil microalgae fix CO through photosynthesis 2 And active substances such as amino acid, sugar, polypeptide, hormone and the like are secreted in the growth and propagation process, so that the growth of other microorganisms can be promoted, and the soil fertility can be synergistically improved.
Example 1:
the embodiment provides a microalgae compound conditioner for improving soil in urban moving places, which comprises the following components in parts by weight:
20-25 parts of combined microalgae solution, wherein the concentration of the microalgae solution is 0.75g/L, and the volume of the microalgae solution is 1L.
20-25 parts of biochar, wherein the mass of the biochar is 1kg.
50-60 parts of bio-organic fertilizer, wherein the mass of the bio-organic fertilizer is 3kg.
Wherein, the liquid crystal display device comprises a liquid crystal display device,
the combined microalgae liquid is formed by mixing nitrogen-fixing blue algae and filamentous blue algae, wherein the nitrogen-fixing blue algae comprises anabaena, nostoc and bifidobacterium, and the filamentous blue algae comprises microcystis vaginalis, microcystis macroalgae and Sphaerotheca malanii. Taking anabaena (algae density 0.6 g/L), nostoc (algae density 0.2 g/L), bifidobacterium (algae density 0.2 g/L), microcystis vaginalis (algae density 0.13 g/L), microcystis martensii (algae density 0.13 g/L) and aschersonia maladshurica (algae density 0.13 g/L) in logarithmic growth phase according to the volume ratio of 1:1:1:1:1:1, mixing to obtain the mixed algae liquid with the required proportion.
As shown in fig. 1, the preparation method of the microalgae compound conditioner for improving the soil of the urban moving land comprises the following steps:
firstly, preparing biochar particles: the biochar prepared from the greening waste is firstly screened, and the aperture is 1cm. If the large particles do not pass through the screen, the large particles are crushed again and then are screened for the second time.
Secondly, preparing a microalgae-biochar compound: separating and concentrating the mixed algae liquid until the algae density is 0.75g/L, preparing 1L of combined microalgae liquid with the concentration of 0.75g/L, adding 1kg of biochar particles, and stirring at the speed of 250rpm for 24 hours at room temperature.
Thirdly, preparing the microalgae-biochar-bio-organic fertilizer conditioner: and uniformly stirring 1kg of the microalgae-biochar compound and 3kg of the bio-organic fertilizer to obtain the microalgae compound conditioner.
The microalgae compound conditioner prepared in the example 1 is subjected to effect verification, and the effect verification is as follows:
the soil to be improved is urban moving land soil taken from Shanghai, and the test development time is as follows: 2021.12.20 to 2022.3.20.
The physical and chemical properties of the relocation soil are shown in Table 1 below.
The test procedure is shown in table 2 below.
Soil fertility index measured after 2 months of soil improvement is shown in table 3 below:
as shown by the test results in the table 3, after the microalgae compound conditioner is applied, the pH and total salt content of the soil are obviously reduced, the saline-alkali barrier of the soil is obviously improved, and meanwhile, the application of the microalgae compound conditioner also obviously improves the contents of organic matters, alkaline nitrogen, quick-acting phosphorus and quick-acting potassium in the soil. The soil fertility improving effect is best when the application amount of the microalgae compound conditioner is 8% in both sample areas.
The application inoculates autotrophic soil microalgae capable of photosynthesis into the soil of the moving place, which can effectively overcome the defect that the soil of the moving place is barren, and the soil microorganisms lack energy and cannot survive. The repair cost of urban relocation soil is low, the soil can be improved after the ground building is removed without subsequent maintenance, and soil microalgae can be used for improving the soil through the growth and propagation process of the soil microalgae. The microalgae compound conditioner is recommended to be popularized and applied in urban green space soil improvement practice.
The foregoing describes specific embodiments of the present application. It is to be understood that the application is not limited to the particular embodiments described above, and that various changes or modifications may be made by those skilled in the art within the scope of the appended claims without affecting the spirit of the application. The embodiments of the application and the features of the embodiments may be combined with each other arbitrarily without conflict.
Claims (3)
1. The application of the microalgae compound conditioner in the improvement of urban relocation soil comprises the following indexes before the remediation of the urban relocation soil:
pH value 8.9 organic matter 5.1g/kg total salt 2.3g/kg alkaline hydrolysis nitrogen 8.5mg/kg
The quick-acting phosphorus 58.9mg/kg quick-acting potassium 29.4mg/kg, which is characterized by comprising the following components in parts by weight:
20-25 parts of combined microalgae liquid
20-25 parts of biochar
50-60 parts of biological organic fertilizer
Wherein, the liquid crystal display device comprises a liquid crystal display device,
the combined microalgae liquid is formed by mixing nitrogen-fixing blue algae and filamentous blue algae, wherein the nitrogen-fixing blue algae comprises anabaena, nostoc and bifidobacterium, and the filamentous blue algae comprises microcystis vaginalis, microcystis macroalgae and sphingale wari; the mixing ratio of the nitrogen-fixing blue algae and the filamentous blue algae in the combined microalgae liquid is 3:2; the nitrogen-fixing blue algae comprises the components of anabaena, nostoc and bifidobacterium in a proportion of 3:1:1, a step of; the filamentous cyanobacteria comprises the components of the microcystis vaginalis, the microcystis martensii and the Sphingomonas maladshurica in the proportion of 1:1:1, a step of;
the biochar is prepared from urban greening waste, and the bio-organic fertilizer is a commercial product conforming to the industry standard NY/T525-2021; the preparation method of the biochar comprises the steps of putting the dried urban greening waste into a microwave muffle furnace for pyrolysis, introducing nitrogen at the flow of 250L/h before heating, then heating to 450 ℃ at the speed of 5 ℃/min, and maintaining the temperature for 1 hour; naturally cooling to 100 ℃, and stopping introducing nitrogen to obtain the prepared biochar; the performance index of the biological organic fertilizer is that the organic matter is more than or equal to 45 percent, the effective viable count is more than or equal to 2 hundred million/g, and N+P 2 O 5 +K 2 O≥5％；
The preparation method of the microalgae compound conditioner comprises the following steps:
firstly, preparing biochar particles: the biochar is subjected to sieving treatment, and the particle diameter of the biochar is required to be less than or equal to 1cm;
secondly, preparing a microalgae-biochar compound: the prepared biochar particles are put into 0.75g/L of combined microalgae liquid which is cultured in advance, and stirred for 24 hours at the room temperature and the rotating speed of 250rpm, so as to obtain microalgae-biochar complex;
thirdly, preparing the microalgae-biochar-bio-organic fertilizer conditioner: mixing the microalgae-biochar compound and the bio-organic fertilizer according to a required proportion to obtain a required conditioner;
the application amount of the microalgae compound conditioner in the urban relocation soil is 8%, and the physicochemical indexes of the urban relocation soil after two months of application are as follows:
pH value is 6.8, organic matter is 27.5g/kg total salt content is 0.8g/kg alkaline hydrolysis nitrogen is 52.3mg/kg quick-acting phosphorus is 96.4mg/kg quick-acting potassium is 79.4mg/kg.
2. The application of the microalgae compound conditioner in improving urban relocation soil according to claim 1, wherein in the first step, the unsieved biochar particles are required to be crushed again, and sieving treatment is performed after crushing.
3. The application of the microalgae compound conditioner in improving urban relocation soil according to claim 1, wherein in the third step, the mixing weight ratio of the microalgae-biochar compound to the bio-organic fertilizer is 1:3.
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