CN102674912B - Biological compound fertilizer and preparation method thereof - Google Patents

Biological compound fertilizer and preparation method thereof Download PDF

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CN102674912B
CN102674912B CN201210159937.4A CN201210159937A CN102674912B CN 102674912 B CN102674912 B CN 102674912B CN 201210159937 A CN201210159937 A CN 201210159937A CN 102674912 B CN102674912 B CN 102674912B
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compound fertilizer
algae
biological compound
aquae
photosynthesis
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CN102674912A (en
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雷云飞
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Liu Xingquan
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Beijing Difulai Science And Technology Developing Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/03Algae
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers

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Abstract

The invention relates to the field of agriculture and in particular relates to a biological compound fertilizer and a preparation method thereof. The biological compound fertilizer is prepared from the following raw materials: blue-green algae with nitrogen fixation and decomposition functions, green algae with a photosynthesis function and red algae with photosynthesis and nitrogen fixation functions. The invention can provide a biological fertilizer which is eutrophic and contains no chemical nutritional ingredient.

Description

Biological compound fertilizer and preparation method thereof
Technical Field
The invention relates to the field of agriculture, in particular to a biological compound fertilizer and a preparation method thereof.
Background
At present, with the development of science and technology, various biological compound fertilizers applied to crops are developed and applied to various crops to effectively promote the growth of the crops, but most of the biological compound fertilizers are produced by chemical substances and can bring chemical pollution to soil and the crops when applied to the crops.
Disclosure of Invention
The invention provides a biological compound fertilizer and a preparation method thereof, which can solve the technical problems.
The invention provides a biological compound fertilizer which comprises the following raw materials: blue algae with nitrogen fixing and decomposing functions, green algae with photosynthesis and red algae with photosynthesis and nitrogen fixing functions.
The blue algae is preferably: anabaena flos-aquae; and/or the presence of a gas in the gas,
the green algae are preferably: chlorella pyrenoidosa; and/or the presence of a gas in the gas,
the red algae are preferably: azotobacter.
In some embodiments, the chlorella pyrenoidosa, the anabaena flos-aquae and the azotobacter are preferably added in the following amounts in parts by weight: 10-50: 10-50: 10 to 70.
Further, in some embodiments, the addition amount of the chlorella pyrenoidosa, the anabaena flos-aquae and the azotobacter is preferably as follows in parts by weight: 20-40: 20-40: 20 to 50.
Still further, in some embodiments, the chlorella pyrenoidosa, the anabaena flos-aquae, and the azotobacter are preferably added in an amount of, in parts by weight: 30: 30: 40.
the addition size of the anabaena flos-aquae is preferably 2-15 mu m; and/or the presence of a gas in the gas,
the addition size of the chlorella pyrenoidosa is preferably 3-5 μm; and/or the presence of a gas in the gas,
the addition size of the azotobacter is preferably 8-15 mu m.
The plant protein content of the chlorella pyrenoidosa is preferably more than 50%, the fat content is preferably 6-7%, and the chlorella pyrenoidosa preferably contains vitamins, cellulose, folic acid, nucleic acid, calcium, magnesium, iron and zinc; and/or the presence of a gas in the gas,
the state of the biological compound fertilizer preferably comprises: liquid or solid.
The invention also provides a preparation method of the biological compound fertilizer, which comprises the following steps:
A. respectively cultivating blue algae with nitrogen fixing and decomposing functions, green algae with photosynthesis and red algae with photosynthesis and nitrogen fixing functions;
B. and mixing the cultivated blue algae, green algae and red algae to prepare the biological compound fertilizer.
Between a and B, the preparation method preferably further comprises: preparing blue algae solution of the blue algae, green algae solution of the green algae and red algae solution of the red algae respectively; then the process of the first step is carried out,
said B preferably comprises: b1, setting the illumination intensity and the heat intensity during mixing respectively; and B2, mixing the blue algae solution, the green algae solution and the red algae solution under the conditions of the light intensity and the heat intensity.
Between the B1 and the B2, the B preferably further comprises: inputting a nitrogen source, a phosphorus source and an inorganic salt solution required by the growth of algae cells, continuously introducing air and intermittently providing carbon dioxide gas;
the B2 preferably further comprises: and mixing the blue algae liquid, the green algae liquid and the red algae liquid under the conditions of the nitrogen source, the phosphorus source and the inorganic salt solution and/or the air and the carbon dioxide gas.
The biological compound fertilizer and the preparation method thereof provided by the invention can achieve the following beneficial effects:
1. the biological compound fertilizer comprises the following raw materials: blue algae with nitrogen fixing and decomposing functions, green algae with photosynthesis and red algae with photosynthesis and nitrogen fixing functions, and the blue algae cells are also called nitrogen fixing cells, so that the blue algae cells have efficient nitrogen fixing and decomposing functions, can fix molecular nitrogen in the atmosphere into combined nitrogen and further synthesize protein, and can wrap plant root systems after the blue algae cells enter soil and extend along with the root systems to provide sufficient nutrients for the roots; the photosynthesis of green algae cells is tens of times that of other plants, the solar energy is absorbed with extraordinary force, and the green algae cells can be photosynthetic under the condition that a visible light source exists; red algae is a kind of prokaryotic algae capable of photosynthesis and nitrogen fixation, and has photosynthetic pigment inside it and photosynthesis under lighting condition. Therefore, the biological compound fertilizer consisting of blue algae with nitrogen fixing and decomposing functions, green algae with photosynthesis and red algae with the photosynthesis and nitrogen fixing functions can wrap the root systems of plants when entering the soil, form a symbiotic interaction relationship with the root systems, fix nitrogen in the air, decompose and dissolve insoluble phosphorus and potassium, activate insoluble calcium, magnesium or sulfur elements, change the insoluble calcium, magnesium or sulfur elements into an effective state which can be absorbed by the plants and provide the effective state for the plants, and directly provide the nutrients for the plants through the symbiotic relationship between microorganisms and the plants, thereby promoting the root system development of the plants and the absorption of the nutrients. On the other hand, the biological compound fertilizer can not only act on the surface of a plant root system to survive and propagate, but also can enter the interior of the plant to survive in the root, stem, leaf and fruit red of the plant, provides rich and balanced natural nutrients for the plant, does not contain any chemical nutrient components, and is a biological fertilizer with rich nutrition.
2. Chlorella pyrenoidosa as a green algae can increase water body dissolved oxygen and degrade water body ammonia nitrogen H2S, nitrite; anabaena flos-aquae, cells are spherical, barrel-shaped and abnormal-shaped cells are intergrown, 1 or more spores are strung against the abnormal-shaped cells or positioned among the abnormal-shaped cells, nitrogen in the air can be assimilated, and the fertility of soil or water is increased. In addition, the anabaena flos-aquae can also carry out photosynthesis by means of sunlight, water and carbon dioxide; the azorhodochrous can not only carry out photosynthesis, but also can carry out respiration and fermentation, can adapt to the change of environmental conditions to change the way of obtaining energy, and simultaneously, the azorhodochrous is a facultative organism and can live in two different metabolic modes of aerobic heterotrophy and optical heterotrophy respectively under the conditions of aerobic darkness and anaerobic illumination.
3. The state of the biological compound fertilizer comprises liquid state or solid state, and the application space and the storage mode of the biological compound fertilizer can be enriched.
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In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other embodiments and drawings can be obtained according to the embodiments shown in the drawings without creative efforts.
FIG. 1 is a flow chart of the preparation of a bio-compound fertilizer according to an embodiment of the present invention.
Detailed Description
The technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is to be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a biological compound fertilizer which comprises the following raw materials: blue algae with nitrogen fixing and decomposing functions, green algae with photosynthesis and red algae with photosynthesis and nitrogen fixing functions.
The cyanobacteria cells are also called nitrogen fixation cells, have efficient nitrogen fixation and decomposition functions, can fix molecular nitrogen in the atmosphere into combined nitrogen, further synthesize protein, can wrap plant root systems after the cyanobacteria cells enter the soil, and extend along with the root systems to provide sufficient nutrients for the roots; the photosynthesis of green algae cells is tens of times that of other plants, the solar energy is absorbed with extraordinary force, and the green algae cells can be photosynthetic under the condition that a visible light source exists; red algae is a kind of prokaryotic algae capable of photosynthesis and nitrogen fixation, and has photosynthetic pigment inside it and photosynthesis under lighting condition. Therefore, the biological compound fertilizer consisting of blue algae with nitrogen fixing and decomposing functions, green algae with photosynthesis and red algae with the photosynthesis and nitrogen fixing functions can wrap the root systems of plants when entering the soil, form a symbiotic interaction relationship with the root systems, fix nitrogen in the air, decompose and dissolve insoluble phosphorus and potassium, activate insoluble calcium, magnesium or sulfur elements, change the insoluble calcium, magnesium or sulfur elements into an effective state which can be absorbed by the plants and provide the effective state for the plants, and directly provide the nutrients for the plants through the symbiotic relationship between microorganisms and the plants, thereby promoting the root system development of the plants and the absorption of the nutrients. On the other hand, the biological compound fertilizer can not only act on the surface of a plant root system to survive and propagate, but also enter the interior of the plant to survive on the root, stem, leaf and fruit red of the plant, so that the biological compound fertilizer provides rich and balanced natural nutrients for the plant, does not contain any chemical nutrient components, comprises high-quality protein consisting of 18 amino acids, a plurality of vitamins, mineral substances, folic acid, chlorophyll, active factors CG.F., algal polysaccharides, nucleic acids and the like, and the nutrient components are helpful for inducing interferon, activating the immunocompetence of NK cells and improving the disease prevention capability and resistance. The biological compound fertilizer of the invention is a fertilizer with rich nutrition. Is a biological fertilizer with rich nutrition
The biological compound fertilizer of the invention is explained in detail below:
the invention provides a biological compound fertilizer which comprises the following raw materials: blue algae with nitrogen fixing and decomposing functions, green algae with photosynthesis and red algae with photosynthesis and nitrogen fixing functions. Wherein,
the blue algae is preferably: anabaena flos-aquae;
anabaena flos-aquae (commonly called blue algae), cyanophyta and prokaryote, and the plant body is a single filament, or an amorphous colloidal block, or a soft film. The algae filaments have the same width or the tips of the ends are thin, and the straight or irregular spiral is bent and provided with a rubber sheath. The cells are spherical and barrel-shaped, and are heterotype cells, 1 or more spores are strung against the heterotype cells or positioned between the heterotype cells, so that nitrogen in the air can be assimilated, and the fertility of soil or water is increased. The nitrogen fixation function is to catalyze molecular nitrogen (N) in the atmosphere by nitrogen fixation enzyme in cyanobacteria cells2) The nitrogen-fixing blue algae is reduced into nitrogen compounds which can be absorbed and utilized by plants, and further synthesized into protein, the nitrogen-fixing blue algae has the effect in nature, is second only to leguminous plants, and the nitrogen-fixing amount per mu of land per year can reach 1-1.5 kg. Anabaena flos-aquae can also be photosynthetic by means of sunlight, water and carbon dioxide.
The green algae are preferably: chlorella pyrenoidosa;
chlorella pyrenoidosa (commonly called green algae) Chlorophyta and eukaryotes are spherical unicellular freshwater algae, are one of the earliest lives on the earth, are high-efficiency photosynthetic plants, grow and reproduce photoautotrophically and are widely distributed. The chlorella pyrenoidosa lives in fresh water, and with the help of sunlight, water and carbon dioxide, the chlorella pyrenoidosa can divide into the vigorous reproductive capacity of 4 cells every 20 hours, continuously converts solar energy into algae containing various nutritional ingredients, and releases a large amount of oxygen in proliferation; and its photosynthetic capacity is more than 10 times higher than that of other plants. Based on this vitality and the high energy nutrients produced, people appreciate that it is a "canned sun". It is also used as the nutrition base of plant, and has vegetable protein content of more than 50%, fat content of 6-7%, and excellent amino acid composition. Contains balanced nutrients including vitamins, cellulose, folic acid, nucleic acid, calcium, magnesium, iron, zinc, etc. The chlorella pyrenoidosa also has the function of repairing cells, can remove the root source of abnormal chromium cell metabolism in vivo, enables the functions of various organs to be recovered to be normal, opens the secondary metabolism of plants, promotes the smooth internal circulation of the plants, and thus greatly improves the quality of crops.
The red algae are preferably: azotobacter sp;
the azotobacteria is a kind of prokaryotic algae capable of photosynthesis and nitrogen fixation, belonging to one kind of photosynthetic nitrogen-fixing algae, and is widely distributed in river, lake, pond and other water areas, and its interior possesses photosynthetic pigment, and can implement photosynthesis under the condition of light, and the facultative organism can also be grown under the condition of dark microaerobic condition. It has very close symbiotic relationship with red duckweed and blue algae, and is interdependent, co-propagated and grown. The red line algae can be photosynthetic algae, and the individual is in the shape of thin line, rod or spiral; the light-capturing pigment is chlorophyll a and chlorophyll b, and also has special aliphatic carotenoid, which makes the algae body show a special red color; the light energy conversion pigment is chlorophyll a and b, the product of energy conversion is Adenosine Triphosphate (ATP), the main carbon source is organic matter and/or CO2. In the respiration, the strong nitrogen fixation function can be exerted. Red line algae, its characteristics areThe body has photosynthetic pigment, and can perform photosynthesis under the condition of illumination, and utilize sunlight to obtain energy. In various types of tests in the laboratory: the red line algae not only can perform photosynthesis, but also can perform respiration and fermentation, and can adapt to the change of environmental conditions to change the way of obtaining energy. Meanwhile, the rhodochrous is a facultative organism, and the rhodochrous can live in two different metabolic modes of aerobic heterotrophy and luminous heterotrophy respectively under the conditions of aerobic darkness and anaerobic illumination.
The nitrogen-fixing rhodochrous has higher application value in the aspects of agriculture, environmental protection, medicine and the like.
(1) Organic nitrogen is provided for crops by utilizing the unique physiological characteristics of azotobacter;
(2) the method can completely decompose residual baits, excreta and the like in fish culture water, improve the ecological environment of aquaculture and reduce ammonia nitrogen, Biochemical Oxygen Demand (BOD) for water purification, particularly for aquaculture;
(3) the photosynthesis of the azotobacter can release hydrogen in the metabolic process, and has the characteristics of high hydrogen production rate, high purity of the produced hydrogen and the like.
In order to better exert the functions of all raw materials in the biological compound fertilizer, the addition amount of all the raw materials is different, wherein: in some embodiments, the chlorella pyrenoidosa, the anabaena flos-aquae and the azotobacter are preferably added in the following amounts in parts by weight: 10-50: 10-50: 10 to 70.
Further, in some embodiments, the addition amount of the chlorella pyrenoidosa, the anabaena flos-aquae and the azotobacter is preferably as follows in parts by weight: 20-40: 20-40: 20 to 50.
Still further, in some embodiments, the chlorella pyrenoidosa, the anabaena flos-aquae, and the azotobacter are preferably added in an amount of, in parts by weight: 30: 30: 40.
in order to better exert the functions of all raw materials in the biological compound fertilizer, the adding sizes of all the raw materials are different, wherein:
the addition size of the anabaena flos-aquae is preferably 2-15 mu m; (micrometers), e.g., 2 μm, 4 μm, 6 μm, 8 μm, 10 μm, 12 μm, 14 μm, 15 μm, or a range between any two of these values;
the addition size of the chlorella pyrenoidosa is preferably 3-5 μm (micrometer), such as 3 μm, 3.5 μm, 4 μm, 4.5 μm, 5 μm or the range between any two values;
the addition size of the azotobacteria is preferably 8-15 μm (micrometer), such as: 8 μm, 9 μm, 10 μm, 11 μm, 12 μm, 13 μm, 14 μm, 15 μm or a range between any two of these.
The plant protein content of the chlorella pyrenoidosa is preferably more than 50%, the fat content is preferably 6-7%, and the chlorella pyrenoidosa preferably contains vitamins, cellulose, folic acid, nucleic acid, calcium, magnesium, iron and zinc;
in order to improve the application of the biological compound fertilizer to different crops and expand the storage mode of the biological compound fertilizer, the state of the biological compound fertilizer preferably comprises the following steps: liquid or solid.
The invention also provides a preparation method of the biological compound fertilizer, which comprises the following steps:
A. respectively cultivating blue algae with nitrogen fixing and decomposing functions, green algae with photosynthesis and red algae with photosynthesis and nitrogen fixing functions;
B. and mixing the cultivated blue algae, green algae and red algae to prepare the biological compound fertilizer.
The method for producing a bio-compound fertilizer according to any one of the above embodiments is described in detail by a specific example, as shown in fig. 1:
101, cultivating blue algae with nitrogen fixation and decomposition functions;
the blue algae is preferably: anabaena flos-aquae.
Step 102, processing blue algae into blue algae liquid;
103, cultivating green algae with photosynthesis;
the green algae are preferably: chlorella pyrenoidosa.
104, processing green algae into green algae liquid;
105, cultivating red algae with the function of photosynthetic nitrogen fixation;
the red algae are preferably: azotobacter.
Step 106, processing the red algae into red algae liquid.
Step 107, setting mixed illumination intensity and heat intensity;
wherein the light intensity and heat intensity can be adjusted by the control box according to empirical values or specific cultured algae.
108, inputting a nitrogen source, a phosphorus source and an inorganic salt solution required by the growth of the algae cells;
step 109, continuously introducing air and intermittently providing carbon dioxide gas;
the gas is gas for helping the growth of the biological compound fertilizer or gas for helping the growth of each raw material of the biological compound fertilizer.
Step 108 and step 109 can be performed simultaneously.
And step 110, mixing the blue algae liquid, the green algae liquid and the red algae liquid, and enabling the blue algae liquid, the green algae liquid and the red algae liquid to grow into the biological compound fertilizer under the conditions of set illumination intensity, heat intensity, nitrogen source, phosphorus source, inorganic salt solution, air and carbon dioxide.
The order of culturing blue-green algae having nitrogen fixing and decomposing functions, green algae having photosynthesis, and red algae having photosynthesis and nitrogen fixing functions may be adjusted or simultaneously cultured in different facilities.
The biological compound fertilizer and the preparation method thereof provided by the invention can achieve the following beneficial effects:
1. the biological compound fertilizer comprises the following raw materials: blue algae with nitrogen fixing and decomposing functions, green algae with photosynthesis and red algae with photosynthesis and nitrogen fixing functions, and the blue algae cells are also called nitrogen fixing cells, so that the blue algae cells have efficient nitrogen fixing and decomposing functions, can fix molecular nitrogen in the atmosphere into combined nitrogen and further synthesize protein, and can wrap plant root systems after the blue algae cells enter soil and extend along with the root systems to provide sufficient nutrients for the roots; the photosynthesis of green algae cells is tens of times that of other plants, the solar energy is absorbed with extraordinary force, and the green algae cells can be photosynthetic under the condition that a visible light source exists; red algae is a kind of prokaryotic algae capable of photosynthesis and nitrogen fixation, and has photosynthetic pigment inside it and photosynthesis under lighting condition. Therefore, the biological compound fertilizer is a fertilizer with rich nutrition.
2. Chlorella pyrenoidosa as a green algae can increase water body dissolved oxygen and degrade water body ammonia nitrogen H2S, nitrite; anabaena flos-aquae, cells are spherical, barrel-shaped and abnormal-shaped cells are intergrown, 1 or more spores are strung against the abnormal-shaped cells or positioned among the abnormal-shaped cells, nitrogen in the air can be assimilated, and the fertility of soil or water is increased. In addition, the anabaena flos-aquae can also carry out photosynthesis by means of sunlight, water and carbon dioxide; the azorhodochrous can not only carry out photosynthesis, but also can carry out respiration and fermentation, can adapt to the change of environmental conditions to change the way of obtaining energy, and simultaneously, the azorhodochrous is a facultative organism and can live in two different metabolic modes of aerobic heterotrophy and optical heterotrophy respectively under the conditions of aerobic darkness and anaerobic illumination.
3. The state of the biological compound fertilizer comprises liquid state or solid state, and the application space and the storage mode of the biological compound fertilizer can be enriched.
The biological compound fertilizer can be used for fruits or crops, wherein:
can improve the sugar content of the fruits, prolong the preservation period, and ensure the uniform size and color of the fruits. See table 1 for details, which shows:
TABLE 1 sugar increment of crops using bio-compound fertilizer
Crops Increase sugar degree Crops Increase sugar degree
Apple (Malus pumila) 0.8-2 Sugarcane 0.8-1
Peach shape 1-2 Mango (mango) 1-2
Kiwi fruit 2 Watermelon 1-2
Lichee 3 Hami melon 1-2
Strawberry 1-3 Chinese date 2
Sugar beet 0.5-2 Grape 0.6-1
Average 0.5-2
Can reduce heavy metal residue, has adsorption effect on heavy metal ions such as cadmium, nickel, lead, tin, copper, zinc, cobalt, manganese and the like, and is shown in table 2:
TABLE 2 reduction of heavy metal content in crops using bio-compound fertilizer
Figure GDA0000400816170000111
The various embodiments provided by the invention can be combined with each other in any way according to the needs, and the technical solution obtained by the combination is also within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention also encompasses these modifications and variations.

Claims (5)

1. The biological compound fertilizer is characterized by comprising the following raw materials: anabaena flos-aquae with nitrogen fixing and decomposing functions, chlorella pyrenoidosa with photosynthesis, and rhodochrous azotoffee with photosynthesis nitrogen fixing function; the addition amounts of the chlorella pyrenoidosa, the anabaena flos-aquae and the azotobacter are as follows according to the parts by weight: 10-50: 10-50: 10-70;
the biological compound fertilizer is prepared by the following preparation method:
respectively cultivating anabaena flos-aquae with nitrogen fixing and decomposing functions, chlorella pyrenoidosa with photosynthesis and nitrogen fixing rhodochrous with photosynthesis and nitrogen fixing functions;
preparing an algae solution of the anabaena flos-aquae, an algae solution of the chlorella pyrenoidosa and an algae solution of the azotobacter respectively;
setting the illumination intensity and the heat intensity during mixing respectively; inputting a nitrogen source, a phosphorus source and an inorganic salt solution required by the growth of algae cells, continuously introducing air and intermittently providing carbon dioxide gas;
and mixing the algae solution of the anabaena flos-aquae, the algae solution of the chlorella pyrenoidosa and the algae solution of the rhodochrous azotobacter under the conditions of the illumination intensity and the heat intensity and the conditions of the nitrogen source, the phosphorus source and the inorganic salt solution and/or the air and the carbon dioxide gas to obtain the biological compound fertilizer.
2. The biological compound fertilizer as claimed in claim 1, wherein the addition amounts of the chlorella pyrenoidosa, the anabaena flos-aquae and the azotobacter are as follows according to the parts by weight: 20-40: 20-40: 20 to 50.
3. The biological compound fertilizer as claimed in claim 2, wherein the addition amounts of the chlorella pyrenoidosa, the anabaena flos-aquae and the azotobacter are as follows according to the parts by weight: 30: 30: 40.
4. a biological compound fertilizer according to any one of claims 1 to 3,
the addition size of the anabaena flos-aquae is 2-15 mu m; and/or the presence of a gas in the gas,
the addition size of the chlorella pyrenoidosa is 3-5 microns; and/or the presence of a gas in the gas,
the addition size of the azotobacter is 8-15 mu m.
5. A biological compound fertilizer according to any one of claims 1 to 3,
the chlorella pyrenoidosa contains plant protein more than 50%, fat 6-7%, vitamins, cellulose, folic acid, nucleic acid, calcium, magnesium, iron and zinc;
and/or the presence of a gas in the gas,
the states of the biological compound fertilizer comprise: liquid or solid.
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WO2018075948A1 (en) * 2016-10-21 2018-04-26 Heliae Development Llc Kappaphycus active ingredient compositions for modulating plant characteristics

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