CN114480121B

CN114480121B - Reaction device suitable for cell growth

Info

Publication number: CN114480121B
Application number: CN202210034494.XA
Authority: CN
Inventors: 张百峰; 段瑞雪; 韩伟; 朱继玲
Original assignee: Shandong Shuifa Life Science Research Co ltd
Current assignee: Shandong Shuifa Life Science Research Co ltd
Priority date: 2022-01-13
Filing date: 2022-01-13
Publication date: 2022-12-09
Anticipated expiration: 2042-01-13
Also published as: CN114480121A

Abstract

The invention relates to a reaction vessel, in particular to a reaction device suitable for cell growth, which comprises a reactor made of stainless steel, wherein a bracket is fixed at the bottom of the reactor, a vertical sliding rod positioned in the middle of the reactor is fixed on the bracket, a disc-shaped stirring disc capable of moving up and down on the sliding rod is arranged on the sliding rod, a sliding hole with the diameter slightly larger than that of the sliding rod is arranged in the center of the stirring disc, the sliding rod and the bracket are made of stainless steel materials, and the stirring disc is made of iron materials; an electromagnet capable of magnetically attracting the stirring disc is arranged above the reactor, a stop switch for stopping the electromagnet is arranged at the upper end of the sliding rod, and an operation switch for operating the electromagnet is arranged at the lower end of the sliding rod. The invention has the advantages of good mixing effect and avoiding damaging cells; can not cause cell pollution.

Description

Reaction device suitable for cell growth

Technical Field

The invention relates to a reaction vessel, in particular to a reaction device suitable for cell growth. .

Background

The reaction conditions required by the cell culture reaction are very strict, and the requirement on environmental cleanliness is very high. In addition, the cellular reaction is different from other chemistry, namely, the culture solution is required to have certain fluidity and cannot be too violent, so that the cells are prevented from being damaged.

At present, in order to fully mix cells and nutrient substances, anchor-type stirring reaction kettles are adopted in reaction containers for cell growth, and the reaction kettles have the defects that firstly, the stirring speed is high, the cells are easily damaged, secondly, the stirring effect is poor, and the culture solution can not be exchanged up and down because the liquid only moves in the circumferential direction. And thirdly, lubricating oil and a packing for sealing are needed for sealing above the stirring shaft, so that the cells are easily polluted and the growth of the cells is influenced.

Disclosure of Invention

In order to overcome the defects, the invention provides a reaction device suitable for cell growth, and overcomes the defects of high stirring speed, poor stirring effect and reaction environment pollution.

The invention is realized by the following measures:

the utility model provides a reaction unit suitable for cell growth, includes the reactor of being made by the stainless steel, is provided with heating device, discharge gate, feed inlet, detection mouth on the reactor, its characterized in that: a support is fixed at the bottom of the reactor, a vertical sliding rod positioned in the middle of the reactor is fixed on the support, a disc-shaped stirring disc capable of moving up and down on the sliding rod is arranged on the sliding rod, a sliding hole with the diameter slightly larger than that of the sliding rod is arranged in the center of the stirring disc, the sliding rod and the support are made of stainless steel materials, and the stirring disc is made of iron materials; an electromagnet capable of magnetically attracting the stirring disc is arranged above the reactor, a stop switch for stopping the electromagnet is arranged at the upper end of the sliding rod, and an operation switch for operating the electromagnet is arranged at the lower end of the sliding rod.

In the above reaction apparatus for cell growth, it is preferable that the stirring plate is coated with a plastic material on the outer circumference thereof in order to prevent rusting and maintain the cleanness of the inside of the reactor.

In the above reaction apparatus for cell growth, preferably, in order to prevent turbulence of the liquid and keep the stirring plate stable, the stirring plate is provided with four circulation holes which are circumferentially and uniformly distributed and have the same diameter.

The reaction device suitable for cell growth described above is preferably: the height of the sliding rod is 0.5-1 times of the height of the reactor, and the diameter of the stirring disc is 0.2-0.5 times of the diameter of the reactor.

Preferably, in order to ensure the stable ascending and descending of the stirring disc, the sliding rods are three or four in parallel, and the stirring disc is provided with three or four sliding holes matched with the sliding rods.

The stirring disc moves upwards along the sliding rod through the attraction of the electromagnet, stops moving when the stirring disc moves to the stop switch at the upper end, moves downwards by means of gravity, and moves upwards next time when the stirring disc slides to the lower end of the sliding rod to operate the switch, and the stirring disc circularly reciprocates.

Because the stirring disc is a horizontal disc, when the stirring disc moves upwards, the reaction liquid above the stirring disc can be driven to move upwards, the reaction liquid around the stirring disc is driven to move downwards, and the reaction liquid at the bottom of the reactor moves upwards, so that the up-and-down exchange of the reaction liquid is completed. When the stirring plate is operated downward, the reaction liquid is operated in reverse. And in each operation period of the stirring disc, the reaction liquid can be operated up and down once, so that the reaction liquid can be well mixed. The stirring disc of the invention can achieve good effect under the condition of slow running.

Four circulation holes are formed in the middle of the stirring disc, so that part of reaction liquid can pass through the circulation holes, and the stirring disc can be guaranteed to stably run up and down. The turbulence of the reaction solution is prevented, and the stirring plate is kept as horizontal as possible.

The sliding rods are three or four in parallel, so that the stirring disc can be kept horizontal better, and deviation is prevented.

The invention has the advantages that through the up-and-down operation of the stirring disc, a better stirring effect is obtained under the condition of slow speed, so that the up-and-down materials are mixed more quickly, and the damage to cells is avoided; because the stirring disc and the sliding rod are arranged in the reaction device, the stirring shaft is not required to be sealed, the pollution of lubricating oil to cells is avoided, and the growth of the cells is ensured.

Drawings

FIG. 1 is a schematic view of the structure of a reaction apparatus according to example 1 of the present invention

FIG. 2 is a schematic top view of the stirring board of embodiment 1 of the present invention

FIG. 3 is a schematic top view of the stirring plate of embodiment 2 of the present invention

In the figure, 1 detection port, 2 feed ports, 3 discharge ports, 4 heating devices, 5 supports, 6 stirring discs, 7 sliding rods, 8 operation switches, 9 stop switches, 10 sliding holes, 11 circulation holes and 12 electromagnets are arranged.

Example 1

The reaction device suitable for cell growth of the invention is shown in figure 1, and comprises a reactor made of stainless steel, wherein the reactor is provided with a heating device 4, a discharge hole 3, a feed inlet 2 and a detection hole 1. The bottom of the reactor is fixed with a bracket 5 which is in a cross shape and fixed on the inner wall of the reactor. A vertical sliding rod 7 positioned in the middle of the reactor is fixed on the bracket 5, the lower end of the sliding rod is fixed on the bracket, and the upper end of the sliding rod is fixed on the inner wall of the upper end of the reactor. The sliding rod 7 is provided with a disc-shaped stirring disc 6 which can move up and down on the sliding rod, the diameter of the stirring disc is 0.3 times of the true diameter of the reactor, and the center of the stirring disc is provided with a sliding hole 10 the diameter of which is slightly larger than that of the sliding rod.

As shown in figure 2, the middle part of the stirring plate is provided with four circulation holes 11, the diameters of the circulation holes are the same, and the circulation holes are uniformly distributed on the circumference. The sliding rod and the bracket are made of stainless steel materials, the stirring disc is made of iron materials, and fluoroplastic materials wrap the periphery of the stirring disc.

An electromagnet 12 for magnetically attracting the stirring disc is arranged above the reactor, and the electromagnet can attract the stirring disc to move upwards when being electrified. The upper end of the slide bar is provided with a stop switch 9 for stopping the electromagnet, and the lower end of the slide bar is provided with an operation switch 8 for operating the electromagnet. After the electromagnet is electrified, the stirring disc is upwards attracted to operate due to electromagnetic attraction, after the stirring disc operates to the stop switch 9 at the upper end, the electromagnet is powered off, the stirring disc operates downwards under the action of gravity, and after the stirring disc operates to the operation switch 8 at the lower end, the electromagnet is powered on, and the stirring disc operates upwards again and reciprocates.

When the stirring disc moves upwards or downwards, the solution in the reactor is driven to move upwards and downwards so as to fully mix the solution. The running speed of the stirring disc can be controlled by adjusting the power of the electromagnet.

Four flow holes 11 are arranged in the middle of the stirring plate, so that a small amount of solution can flow through the flow holes, the solution turbulence can be prevented, and the stirring plate can stably run upwards or downwards.

The reaction device is used for cell culture, nutrient solution and a certain amount of cultured cells are added, the electromagnet is electrified to drive the stirring disc to move upwards at the upward running speed of about 30mm/s and drive the liquid to turn over up and down, after the stop switch is operated, the electromagnet is powered off, the stirring disc runs downwards under the action of gravity at the running speed of about 20 mm/s. By culturing in the reaction apparatus, the cells can be well grown.

Example 2

The embodiment 2 of the invention is basically the same as the embodiment 1, and the difference is that the stirring disc comprises four sliding holes 10 which are uniformly distributed in the circumferential direction, and similarly, the number of the sliding rods 7 on the bracket is four, and the four sliding rods are matched with the sliding holes, so that the stirring disc can run up and down on the sliding rods. The four sliding rods are arranged, so that the stirring disc can run up and down more stably. 2. The stirring disc is not provided with a circulating hole any more. 3. The diameter of the stirring plate was 0.4 times the diameter of the reactor. 4. The height of the slide bar was 0.8 times the height of the reactor.

Claims

1. The utility model provides a reaction unit suitable for cell growth, includes the reactor of being made by the stainless steel, is provided with heating device, discharge gate, feed inlet, detection mouth on the reactor, its characterized in that: a support is fixed at the bottom of the reactor, a vertical sliding rod positioned in the middle of the reactor is fixed on the support, a disc-shaped stirring disc capable of moving up and down on the sliding rod is arranged on the sliding rod, a sliding hole with the diameter slightly larger than that of the sliding rod is arranged in the center of the stirring disc, the sliding rod and the support are made of stainless steel materials, and the stirring disc is made of iron materials; an electromagnet capable of magnetically attracting the stirring disc is arranged above the reactor, a stop switch for stopping the electromagnet is arranged at the upper end of the sliding rod, and an operation switch for operating the electromagnet is arranged at the lower end of the sliding rod;

the height of the sliding rod is 0.5-1 times of the height of the reactor, and the diameter of the stirring disc is 0.2-0.5 times of the diameter of the reactor;

the sliding rods are three or four in parallel, and the stirring disc is provided with three or four sliding holes matched with the sliding rods.

2. A reaction device adapted for cell growth according to claim 1, wherein: the periphery of the stirring plate is wrapped with a plastic material.