CN214572029U - Insect cell co-culture device - Google Patents

Abstract

The utility model discloses an insect cell co-culture device, including cultivateing the chamber, it runs through and is provided with first bull stick and second bull stick to cultivate chamber inner chamber top, cultivate the fixed motor that is provided with in one side that the chamber top is close to first bull stick, motor output shaft tip is connected with first bull stick transmission, first bull stick top and the equal fixed belt pulley that is provided with in second bull stick top, two be connected with driving belt between the belt pulley, first bull stick outer peripheral face bottom is connected with first gear, second bull stick outer peripheral face top is connected with the second gear, it is provided with the carousel to cultivate chamber inner chamber top, the carousel both sides all run through and are provided with the (mixing) shaft. The utility model discloses a motor drives the stirring paddle leaf of both sides and is the consequent rotation when reverse annular removes, realizes the all-round stirring of culture solution system to cultivateing intracavity chamber for newly all kinds of components of supplementing can be rapidly with original culture solution system misce bene, in order to do benefit to the better cultivation of insect cell.

Description

Insect cell co-culture device

Technical Field

The utility model relates to an insect cell culture technical field, concretely relates to insect cell co-culture device.

Background

At present, a method of culturing various cells on a large scale using a cell culture tank or the like has been widely used. For example, in recent years, many researchers have used various serum-free media to culture sf 9-line insect cells and the like for efficient expression of certain functional proteins. In general, during the cultivation of insect cells, the composition (oxygen content, pH value) of the culture solution is continuously monitored, and fresh culture solution is timely supplemented into the culture tank. However, in large-scale culture, a large-sized culture tank is required, and the supplemented fresh culture solution cannot be uniformly mixed with the original culture solution system in a short time, so that the components in each part of the culture system are unbalanced, thereby affecting the cell culture effect.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an insect cell co-culture device, the stirring paddle leaf that drives both sides through the motor is the consequent rotation when reverse annular removes to the realization is to the all-round stirring of the liquid serum-free biological medium of cultivateing the intracavity chamber, makes newly all kinds of components of supplanting can be rapidly with original culture fluid system misce bene, in order to do benefit to the better cultivation of insect cell, with the problem among the above-mentioned background art of solution.

In order to achieve the above object, the present invention provides the following technical solutions: a combined insect cell culture device comprises a culture chamber, wherein a first rotating rod and a second rotating rod are arranged at the top of an inner cavity of the culture chamber in a penetrating mode, a motor is fixedly arranged on one side, close to the first rotating rod, of the top of the culture chamber, the end portion of an output shaft of the motor is in transmission connection with the first rotating rod, belt pulleys are fixedly arranged at the top of the first rotating rod and the top of the second rotating rod, a transmission belt is connected between the two belt pulleys, a first gear is connected to the bottom of the outer peripheral surface of the first rotating rod, a second gear is connected to the top of the outer peripheral surface of the second rotating rod, a rotating disc is arranged at the top of the inner cavity of the culture chamber, stirring shafts are arranged on two sides of the rotating disc in a penetrating mode, third gears are connected to the tops of the outer peripheral surfaces of the two stirring shafts, a chain is connected between the second gear and the two third gears, a gear ring is connected to the top of the rotating disc, and the first gear is meshed with the gear ring, and two stirring blades are fixedly arranged at the bottoms of the stirring shafts.

Preferably, the second rotating rod is arranged at the center of the top of the culture cavity in a penetrating manner.

Preferably, two stirring shafts are axially symmetrically arranged on the vertical central axis of the culture cavity.

Preferably, the second rotating rod and the joints of the two stirring shafts and the turntable are provided with bearings, and the second rotating rod and the two stirring shafts are rotatably connected with the turntable through the bearings.

Preferably, one side of the top of the culture cavity, which is far away from the motor, is fixedly provided with a feed inlet and a feed hopper, and the feed hopper is arranged outside the feed inlet.

Preferably, one side of the bottom of the culture cavity, which is close to the motor, is fixedly provided with a discharge hole and a discharge pipe, the discharge pipe is arranged outside the discharge hole, and the outside of the discharge pipe is in threaded connection with a sealing cover.

Preferably, it has the annular ring to cultivate chamber top inner wall connection, the fixed a plurality of arc walls that are provided with in annular ring top, it is a plurality of the arc wall is the annular and distributes, and is a plurality of all roll in the arc wall and be provided with the ball, it is a plurality of the ball top all laminates with the carousel mutually.

Preferably, four support legs are fixedly arranged at the bottom of the culture cavity, and the included angle between any two adjacent support legs is 90 degrees.

In the technical scheme, the utility model provides a technological effect and advantage:

drive first pivot and first gear clockwise rotation through the motor, first pivot clockwise rotation drives second pivot and second gear clockwise rotation, first gear clockwise rotation drives ring gear and carousel anticlockwise rotation, carousel anticlockwise rotation drives two (mixing) shafts and the stirring paddle leaf that corresponds the position and is reverse annular move, second gear clockwise rotation drives two third gears and the (mixing) shaft that corresponds the position, stirring paddle leaf is the forward rotation simultaneously, stirring paddle leaf on both sides is forward rotation when reverse annular move, thereby realize the all-round stirring of culture solution system to cultivateing the chamber inner chamber, make newly all kinds of components of supplanting can be rapidly with original culture solution system misce bene, in order to do benefit to the better cultivation of insect cell.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to these drawings.

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is an enlarged view of a portion a of fig. 1 according to the present invention.

Fig. 3 is an enlarged view of a portion B of fig. 1 according to the present invention.

Fig. 4 is a schematic view of the connection between the first gear and the gear ring according to the present invention.

Description of reference numerals:

1. a culture chamber; 2. a first rotating lever; 3. a second rotating rod; 4. a motor; 5. a belt pulley; 6. a drive belt; 7. a first gear; 8. a second gear; 9. a turntable; 10. a stirring shaft; 11. a third gear; 12. a chain; 13. a ring gear; 14. a stirring paddle; 15. a feed inlet; 16. a feed hopper; 17. a discharge port; 18. a discharge pipe; 19. sealing the cover; 20. an annular ring; 21. an arc-shaped slot; 22. and a ball.

Detailed Description

Example embodiments will now be described more fully with reference to the accompanying drawings. Example embodiments may, however, be embodied in many different forms and should not be construed as limited to the examples set forth herein; rather, these example embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of example embodiments to those skilled in the art. The drawings are merely schematic illustrations of the present disclosure and are not necessarily drawn to scale. The same reference numerals in the drawings denote the same or similar parts, and thus their repetitive description will be omitted.

Furthermore, the described features, structures, or characteristics may be combined in any suitable manner in one or more example embodiments. In the following description, numerous specific details are provided to give a thorough understanding of example embodiments of the disclosure. One skilled in the relevant art will recognize, however, that the subject matter of the present disclosure can be practiced without one or more of the specific details, or with other methods, components, steps, and so forth. In other instances, well-known structures, methods, implementations, or operations are not shown or described in detail to avoid obscuring aspects of the disclosure.

The utility model provides an insect cell co-culture device as shown in figures 1-4, which comprises a culture chamber 1, wherein a first rotating rod 2 and a second rotating rod 3 are arranged at the top of the inner chamber of the culture chamber 1 in a penetrating way, a motor 4 is fixedly arranged at one side of the top of the culture chamber 1 close to the first rotating rod 2, the end part of an output shaft of the motor 4 is in transmission connection with the first rotating rod 2, belt pulleys 5 are fixedly arranged at the top of the first rotating rod 2 and the top of the second rotating rod 3, a transmission belt 6 is connected between the two belt pulleys 5, a first gear 7 is connected at the bottom of the outer peripheral surface of the first rotating rod 2, a second gear 8 is connected at the top of the outer peripheral surface of the second rotating rod 3, a rotary disc 9 is arranged at the top of the inner chamber of the culture chamber 1, a stirring shaft 10 is arranged at both sides of the rotary disc 9 in a penetrating way, and a third gear 11 is connected at the top of the outer peripheral surface of the two stirring shafts 10, a chain 12 is connected between the second gear 8 and the two third gears 11, a gear ring 13 is connected to the top of the rotary table 9, the first gear 7 is meshed with the gear ring 13, and two stirring blades 14 are fixedly arranged at the bottom of the stirring shaft 10.

Further, in the above technical solution, the second rotating rod 3 is penetratingly arranged at the center of the top of the culture chamber 1.

Further, in the above technical solution, two stirring shafts 10 are axially symmetrically arranged with respect to the vertical central axis of the culture chamber 1.

Further, in the above technical solution, bearings are disposed at the joints of the second rotating rod 3 and the two stirring shafts 10 and the rotating disc 9, and the second rotating rod 3 and the two stirring shafts 10 are rotatably connected with the rotating disc 9 through the bearings.

Further, in the above technical scheme, one side of cultivating chamber 1 top and keeping away from motor 4 is fixed and is provided with feed inlet 15 and feeder hopper 16, feeder hopper 16 sets up in feed inlet 15 outside, the feeding of being convenient for.

Further, in the above technical scheme, one side of the bottom of the culture chamber 1, which is close to the motor 4, is fixedly provided with a discharge hole 17 and a discharge pipe 18, the discharge pipe 18 is arranged outside the discharge hole 17, and a seal cover 19 is in threaded connection with the outside of the discharge pipe 18, so that the seal cover 19 is unscrewed, and discharge is facilitated.

Further, in above-mentioned technical scheme, cultivate 1 top inner wall connection in chamber has annular ring 20, the fixed a plurality of arc walls 21 that are provided with in annular ring 20 top, it is a plurality of arc wall 21 is the annular and distributes, and is a plurality of all roll in the arc wall 21 and be provided with ball 22, it is a plurality of ball 22 top all laminates mutually with carousel 9, and the carousel 9 of being convenient for rotates, improves carousel 9 pivoted stability simultaneously.

Further, in the above technical scheme, four support legs are fixedly arranged at the bottom of the culture cavity 1, and any two adjacent support legs have an included angle of 90 degrees, so that the culture cavity 1 is supported.

The implementation mode is specifically as follows: in practical use, a liquid serum-free biological culture medium and insect cells are added into a culture cavity 1 through a feed hopper 16, a motor 4 is connected with an external power supply through a lead, the motor 4 is connected to drive a first rotating shaft and a first gear 7 to rotate clockwise, under the action of two belt pulleys 5 and a transmission belt 6, the first rotating rod 2 drives a second rotating rod 3 and a second gear 8 to rotate clockwise, the first gear 7 is meshed with a gear ring 13, the first gear 7 rotates clockwise to drive the gear ring 13 and a rotating disc 9 to rotate anticlockwise, the rotating disc 9 further drives two stirring shafts 10 and stirring blades 14 at corresponding positions to do reverse annular movement, because a chain 12 is connected between the second gear 8 and two third gears 11, under the action of the chain 12, the second gear 8 drives the two third gears 11 and the stirring shafts 10 and the stirring blades 14 at corresponding positions to simultaneously rotate clockwise, stirring paddle 14 of both sides is the consequent rotation when reverse annular removes, thereby realize the all-round stirring of the liquid serum-free biological culture base member system of culture chamber 1 inner chamber, make newly-supplemented all kinds of components can be rapidly with original culture fluid system misce bene, in order to do benefit to the better cultivation of insect cell, this embodiment has specifically solved exist among the prior art can not realize all-round stirring to liquid serum-free biological culture medium with ordinary agitating unit, liquid serum-free biological culture medium and insect cell misce bene, thereby be unfavorable for the better problem of cultivateing of insect cell.

While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the present invention. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the invention.

Claims (8)

1. An insect cell co-culture device, includes culture chamber (1), its characterized in that: the cultivation device is characterized in that a first rotating rod (2) and a second rotating rod (3) are arranged at the top of an inner cavity of a cultivation cavity (1) in a penetrating mode, a motor (4) is fixedly arranged on one side, close to the first rotating rod (2), of the top of the cultivation cavity (1), the end portion of an output shaft of the motor (4) is in transmission connection with the first rotating rod (2), belt pulleys (5) are fixedly arranged at the top of the first rotating rod (2) and the top of the second rotating rod (3), a transmission belt (6) is connected between the two belt pulleys (5), a first gear (7) is connected to the bottom of the outer peripheral surface of the first rotating rod (2), a second gear (8) is connected to the top of the outer peripheral surface of the second rotating rod (3), a turntable (9) is arranged at the top of the inner cavity of the cultivation cavity (1), stirring shafts (10) are arranged on two sides of the turntable (9) in a penetrating mode, and a third gear (11) is connected to the top of the outer peripheral surface of the stirring shafts (10), be connected with chain (12) between second gear (8) and two third gear (11), carousel (9) top is connected with ring gear (13), first gear (7) mesh mutually with ring gear (13), two (mixing) shaft (10) bottom all is fixed and is provided with two stirring paddle (14).

2. An insect cell co-culture apparatus according to claim 1, wherein: the second rotating rod (3) penetrates through the center of the top of the culture cavity (1).

3. An insect cell co-culture apparatus according to claim 1, wherein: the two stirring shafts (10) are arranged in axial symmetry with respect to the vertical central axis of the culture cavity (1).

4. An insect cell co-culture apparatus according to claim 1, wherein: the second rotating rod (3) and the joints of the two stirring shafts (10) and the turntable (9) are provided with bearings, and the second rotating rod (3) and the two stirring shafts (10) are rotatably connected with the turntable (9) through the bearings.

5. An insect cell co-culture apparatus according to claim 1, wherein: cultivate one side fixed feed inlet (15) and feeder hopper (16) that motor (4) were kept away from at chamber (1) top, feeder hopper (16) set up in feed inlet (15) outside.

6. An insect cell co-culture apparatus according to claim 1, wherein: cultivate one side fixed discharge gate (17) and the row of being provided with material pipe (18) of chamber (1) bottom near motor (4), arrange material pipe (18) and set up in discharge gate (17) outside, just it has closing cap (19) to arrange material pipe (18) outside threaded connection.

7. An insect cell co-culture apparatus according to claim 1, wherein: cultivate chamber (1) top inner wall connection and have annular ring (20), annular ring (20) top is fixed to be provided with a plurality of arc walls (21), and is a plurality of arc wall (21) are the annular and distribute, and are a plurality of all roll in arc wall (21) and be provided with ball (22), and are a plurality of ball (22) top all laminates mutually with carousel (9).

8. An insect cell co-culture apparatus according to claim 1, wherein: four support legs are fixedly arranged at the bottom of the culture cavity (1), and the included angle between every two adjacent support legs is 90 degrees.

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