CN220012670U - Cell culture device with perfusion function - Google Patents
Cell culture device with perfusion function Download PDFInfo
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- CN220012670U CN220012670U CN202320467188.5U CN202320467188U CN220012670U CN 220012670 U CN220012670 U CN 220012670U CN 202320467188 U CN202320467188 U CN 202320467188U CN 220012670 U CN220012670 U CN 220012670U
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- culture
- cup body
- cell culture
- cell
- perfusion function
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- 238000004113 cell culture Methods 0.000 title claims abstract description 37
- 230000010412 perfusion Effects 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 238000003756 stirring Methods 0.000 claims description 25
- 239000007789 gas Substances 0.000 claims description 22
- 230000003287 optical effect Effects 0.000 claims description 15
- 239000012528 membrane Substances 0.000 claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 238000004090 dissolution Methods 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 22
- 239000000243 solution Substances 0.000 description 16
- 230000010261 cell growth Effects 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 4
- 239000012530 fluid Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 2
- 230000005291 magnetic effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000002912 waste gas Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000005294 ferromagnetic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture device with a perfusion function, which comprises a culture cup body, wherein the middle part of the bottom of the cup body protrudes upwards to extend to form a support structure, the middle part of the support structure is provided with a liquid outlet channel, and the liquid outlet channel extends to the outside of the culture cup body along the bottom of the cup body and protrudes outwards to form a liquid outlet; the bottom of the culture cup body is provided with a liquid inlet channel, and the liquid inlet channel extends to the outside of the culture cup body along the cup bottom and protrudes outwards to form a liquid inlet. The upper part of the supporting structure is fixedly connected with a disc-shaped interception device, the hollow part of the surface of the interception device is integrally connected with a cell interception film, and the cell interception film is a micron-sized filter screen, so that old culture solution can be directly pumped away and cells can be intercepted under the sealed state of the culture device, and high-density cell culture is realized.
Description
Technical Field
The utility model belongs to the technical field of cell culture, and particularly relates to a cell culture device with a perfusion function.
Background
Conventional cell culture devices such as cell culture dishes, cell culture bottles and cell factories cannot realize high-density cell culture due to the fact that effective nutrient addition, waste culture solution removal and timely gas supplement and exchange cannot be realized, and the problems of large investment, multiple technological processes and the like are caused only by obtaining required cell quantity after batch culture and concentration for many times. Meanwhile, when the cell expansion culture is carried out, power is required to be provided for the suspended cells to enable the suspended cells to be in a suspended state, so that the suspended cells can fully contact with nutrient substances in the culture solution, and the cells are uniformly distributed in the culture solution. The existing method is to put it in a stirred reactor or on a shaker to enhance the surface penetration of nutrients, for example:
CN217265751U discloses an instrument for controlling the cell expansion culture inoculation density, wherein the stirring mechanism can stir the mixed solution slowly, so that the suspension, the culture solution and the cells are mixed fully and uniformly.
However, when the culture solution is replaced by the technology, the device is required to be opened, gas cannot be timely supplemented, and the top needs to be provided with holes to be connected with the rotating shaft of the paddle, so that the pollution risk is increased, and the experimental result is greatly influenced.
Disclosure of Invention
In order to solve the problems, the utility model provides a cell culture device with a perfusion function, which can reduce pollution risk, improve cell expansion quality and realize high-density cell culture.
In order to at least achieve one of the above purposes, the present utility model adopts the following technical scheme:
the cell culture device with the perfusion function comprises a culture cup body, wherein the middle part of the bottom of the culture cup body protrudes upwards to form a supporting structure. A liquid outlet channel is formed in the middle of the supporting structure, extends to the outside of the culture cup body along the cup bottom, and protrudes outwards to form a liquid outlet; the bottom of the culture cup body is provided with a liquid inlet channel, and the liquid inlet channel extends to the outside of the culture cup body along the cup bottom and protrudes outwards to form a liquid inlet. The upper part of the supporting structure is fixedly connected with a interception device, the inside of the interception device is of a cavity structure, and a cell interception membrane is integrally connected with the surface hollowed-out part of the interception device.
Further, an optical patch is attached to the bottom of the culture cup body, preferably, the optical patch is an oxygen dissolution value optical patch and an acid-base value optical patch, and the status of the culture solution can be monitored and fed back.
Further, the entrapping device is disc-shaped, and the cell entrapping membrane is a micron-sized filter to prevent cultured cells from entering the liquid outlet channel, preferably, the micron-sized filter has a pore size of 0.5 to 10 μm, and may be any value or any range of the above numerical ranges, for example, 1, 1.5, 2.3, 3.3, 4.1, 5, 6.1, 6.8, 7.6, 8.1, 8.4, 9.3, 9.5, 10 μm, etc., or 0.5 to 1.4, 1 to 3, 2 to 3.3, 4.5 to 8.8, 7 to 10 μm, etc.
Further, a gas outlet and a gas inlet are formed in the side wall of the culture cup body.
Further, the support structure is 10-30mm higher than the bottom of the culture cup.
Further, the upper part of the culture cup body is detachably connected with a culture cover body, and the lower surface of the culture cover body is fixedly provided with a stirring paddle.
Furthermore, the blades of the stirring paddles are of a spiral structure, so that the liquid in the culture cup body flows more uniformly and more slowly, the influence of the shear stress in the fluid on cells is reduced, and the number of the blades of the stirring paddles is 2-6.
Furthermore, a hollow blade opening is formed in the middle of the stirring paddle.
Further, driven magnets are embedded above the stirring paddles.
Furthermore, a sliding gasket is arranged between the culture cover body and the stirring paddle, and the sliding gasket is made of a material with a smooth and wear-resistant surface (such as polytetrafluoroethylene) so as to reduce the friction force between the paddle and the culture cover body.
Still further, cell culture apparatus with perfusion function still includes drive arrangement, drive arrangement is attached in cultivate the upper surface of lid, drive arrangement's below is fixed with driving magnet, with driven magnetism ferromagnetic attraction connects, and to stirring rake transmission power.
Compared with the prior art, the utility model has the following beneficial effects:
1) The utility model adopts the design of the interception device and the micron-level cell interception membrane, and can directly extract the old culture solution and intercept cells in the sealed state of the culture device by the structure, thereby realizing high-density cell culture.
2) The gas inlet and the gas outlet arranged on the device can timely discharge waste gas and convey gas, and provide a gas environment required by cell culture.
3) The bottom of the culture cup body is attached with the dissolved oxygen value optical patch and the acid-base value optical patch, so that the state of the culture solution can be monitored and fed back.
4) The device is driven by non-contact magnetic force, and the magnet is used for transmitting power to the blade in the culture cup body, so that dynamic sealing is not needed, pollution risk is reduced, and reliability is high.
5) According to the utility model, the stirring paddle blade adopts a hollow spiral design, and the rotation stress surface is optimized and structurally avoided to ensure that the liquid in the culture cup body flows more uniformly and more slowly, so that the influence of the shear stress in the fluid on cells is reduced, and the cell expansion quality is improved.
Drawings
Fig. 1 is a schematic perspective view of embodiment 1 of the present utility model;
FIG. 2 is a schematic view showing the structure of a culture cup according to example 1 of the present utility model;
FIG. 3 is an exploded view of example 1 of the present utility model;
fig. 4 is a schematic perspective view of embodiment 2 of the present utility model;
the components in the figures are labeled as follows: 1-culture cup, 11-supporting structure, 12-liquid outlet channel, 13-liquid outlet, 14-liquid inlet channel, 15-liquid inlet, 16-optical patch, 17-gas outlet, 18-gas inlet, 2-interception device, 21-cell interception film, 3-culture cover, 31-stirring paddle, 311-driven magnet, 312-paddle opening and 32-sliding gasket.
Detailed Description
The advantages and features of the present utility model will become more apparent from the following description when taken in conjunction with the accompanying drawings. It should be understood that the embodiments are illustrative only and should not be taken as limiting the scope of the utility model.
It should be noted that, in the claims of the present document, the terms "top," "bottom," "side," "upper," "lower," and the like indicate an orientation or a positional relationship based on that shown in the drawings, and are not necessarily to be construed as necessarily referring to the device or component in a particular orientation, configuration or operation in a particular orientation.
In the present utility model, unless explicitly specified and limited otherwise, the terms "fixedly attached," "connected," and the like are to be construed broadly and, for example, as a fixed connection, as a removable connection, or as a unit; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
Example 1:
fig. 1 to 3 show a specific structure of embodiment 1 of the present utility model, wherein fig. 1 is a schematic perspective view of the present utility model, fig. 2 is a schematic view of the structure of the culture cup of the present utility model, and fig. 3 is an exploded view of the present utility model.
The cell culture device with the perfusion function comprises a culture cup body 1, wherein the middle part of the cup bottom protrudes upwards to form a supporting structure 11, the middle part of the supporting structure 11 is provided with a liquid outlet channel 12, and the liquid outlet channel 12 extends to the outside of the culture cup body 1 along the cup bottom and protrudes outwards to form a liquid outlet 13; the bottom of the culture cup body 1 is provided with a liquid inlet channel 14, and the liquid inlet channel 14 extends to the outside of the culture cup body 1 along the cup bottom and protrudes outwards to form a liquid inlet 15. The upper part of the supporting structure 11 is fixedly connected with a retaining device 2, the retaining device 2 is disc-shaped, the inside is of a cavity structure, a cell retaining membrane 21 is integrally connected with the surface hollowed-out part of the retaining device 2, and the cell retaining membrane 21 is fixed on the surface of the retaining device 2 in a hot melting or ultrasonic welding mode. The cell interception film 21 is a micron-sized filter screen, the aperture is 1 mu m, and only culture solution is discharged during liquid exchange. The upper part of the culture cup body 1 can be detachably connected with a culture cover body 3, and the side wall of the culture cup body 1 is provided with a gas outlet 17 and a gas inlet 18, so that waste gas and conveying gas can be timely discharged, and a gas environment required by cell culture is provided.
In addition, an optical patch 16 is attached to the bottom of the culture cup body 1, and the optical patch 16 is an oxygen dissolution value optical patch and an acid-base value optical patch, so that the state of the culture solution can be monitored and fed back.
In order to realize high-density cell culture, the cells are suspended to be fully contacted with the cell culture solution, the lower surface of the culture cover body 3 is detachably connected with a stirring paddle 31, the paddles of the stirring paddle 31 are of a spiral structure, and a hollow paddle opening 312 is formed in the middle of the stirring paddle. The cell culture device with perfusion function adopts non-contact magnetic drive, a driven magnet 311 is embedded above the stirring paddle 31, and a sliding gasket 32 is arranged between the culture cover body 3 and the stirring paddle 31.
In this embodiment, the number of paddles is 2, and the rotation stress surface of the stirring paddle 31 is optimized and structurally avoided to a certain extent, so that the interception device 2 is avoided, and the hollow-out strip-shaped opening is arranged in the middle of the paddle, so that the liquid flows more uniformly and more slowly, and the influence of the shear stress in the fluid on the cell culture is reduced. The gas outlet 17 and the gas inlet 18 are arranged on the same side of the culture cup body 1 and are integrally formed, so that the device structure is simpler.
The cell culture device with perfusion function of the utility model further comprises a driving device, wherein the driving device is attached to the upper surface of the culture cover body 3, a driving magnet is fixed below the driving device, is magnetically connected with the driven magnet 311, and transmits power to the stirring paddle 31.
Example 2:
FIG. 4 shows a specific structure of example 2 of the cell culture apparatus with perfusion function according to the present utility model.
In this embodiment, the gas outlet 17 and the gas inlet 18 are formed on opposite sides of the culture cup 1, and the liquid outlet 13 and the liquid inlet 15 are formed on opposite sides of the culture cup 1, so that laboratory staff can reasonably arrange the positions of the pipelines according to laboratory environments.
In order to better understand the technical scheme of the utility model, the following uses the amplification process of high-density culture of suspension cells as an example:
1) Under aseptic environment, connect aseptic pipeline to gas outlet, gas inlet, liquid outlet, inlet respectively, pour into cell culture solution and cell into the culture cup body, adsorb drive arrangement to cultivate the lid, cover cultivate the lid and inspect the leakproofness.
2) The motor of the driving device is in a working state, the cell name is marked, then the cell is placed and cultured, the dissolved oxygen value and the acid-base value of the culture solution are monitored through the optical patch, and the culture solution is replaced and ventilated periodically. The old culture solution is pumped away at regular time or continuously and slowly, and simultaneously fresh and nutrient-rich cell culture solution is supplemented into the culture cup at regular time or continuously and slowly, so that continuous long-time culture and proliferation of cells in the culture cup are realized.
3) After more than ten days, the high-density cell expansion is finished, and the device is subjected to aseptic cleaning and disinfection for the next use.
The above description is only of the preferred embodiments of the present utility model and is not intended to limit the present utility model, and various modifications and variations may be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (10)
1. A cell culture apparatus with perfusion function, characterized in that: the culture cup comprises a culture cup body (1), wherein the middle part of the bottom of the culture cup body (1) protrudes upwards to extend to form a support structure (11);
a liquid outlet channel (12) is formed in the middle of the supporting structure (11), and the liquid outlet channel (12) extends to the outside of the culture cup body (1) along the cup bottom and protrudes outwards to form a liquid outlet (13);
a liquid inlet channel (14) is formed in the bottom of the culture cup body (1), and the liquid inlet channel (14) extends to the outside of the culture cup body (1) along the bottom of the cup and protrudes outwards to form a liquid inlet (15);
the device is characterized in that a retaining device (2) is fixedly connected above the supporting structure (11), a cavity structure is arranged inside the retaining device (2), a hollowed-out opening is formed in the surface of the retaining device (2), and a cell retaining membrane (21) is integrally connected to the hollowed-out portion.
2. The cell culture apparatus with perfusion function according to claim 1, wherein: an optical patch (16) is attached to the bottom of the culture cup body (1), and the optical patch (16) is an oxygen dissolution value optical patch and/or an acid-base value optical patch.
3. The cell culture apparatus with perfusion function according to claim 1, wherein: the interception device (2) is disc-shaped, the cell interception membrane (21) is a micron-sized filter screen, and the pore diameter of the filter screen is 0.5-10 mu m.
4. The cell culture apparatus with perfusion function according to claim 1, wherein: the side wall of the culture cup body (1) is provided with a gas outlet (17) and a gas inlet (18).
5. The cell culture apparatus with perfusion function according to claim 1, wherein: the upper part of the culture cup body (1) is detachably connected with a culture cover body (3), and the lower surface of the culture cover body (3) is fixedly provided with a stirring paddle (31).
6. The cell culture apparatus with perfusion function according to claim 5, wherein: a driven magnet (311) is embedded above the stirring paddle (31).
7. The cell culture apparatus with perfusion function according to claim 6, wherein: hollow blade holes (312) are formed in the middle of the stirring paddle (31).
8. The cell culture apparatus with perfusion function according to claim 7, wherein: a sliding gasket (32) is arranged between the culture cover body (3) and the stirring paddle (31).
9. The cell culture apparatus with perfusion function according to claim 8, wherein: the blades of the stirring paddles (31) are of a spiral structure, and the number of the blades of the stirring paddles (31) is 2-6.
10. The cell culture apparatus with perfusion function according to claim 9, wherein: the cell culture device with the perfusion function further comprises a driving device, the driving device is attached to the upper surface of the culture cover body (3), a driving magnet is fixed below the driving device, is magnetically connected with the driven magnet (311), and transmits power to the stirring paddle (31).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320467188.5U CN220012670U (en) | 2023-03-13 | 2023-03-13 | Cell culture device with perfusion function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320467188.5U CN220012670U (en) | 2023-03-13 | 2023-03-13 | Cell culture device with perfusion function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220012670U true CN220012670U (en) | 2023-11-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320467188.5U Active CN220012670U (en) | 2023-03-13 | 2023-03-13 | Cell culture device with perfusion function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220012670U (en) |
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2023
- 2023-03-13 CN CN202320467188.5U patent/CN220012670U/en active Active
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