CN217103837U

CN217103837U - High-efficient culture apparatus of bacillus

Info

Publication number: CN217103837U
Application number: CN202220129451.5U
Authority: CN
Inventors: 种婷
Original assignee: Liaoning Inspection Examination and Certification Centre
Current assignee: Liaoning Inspection Examination and Certification Centre
Priority date: 2022-01-18
Filing date: 2022-01-18
Publication date: 2022-08-02
Anticipated expiration: 2032-01-18

Abstract

The utility model discloses a high-efficient culture apparatus of bacillus, including cultivateing the bucket, symmetric connection has four first breather pipes on the ring canal that cultivates the bucket upper end outer setting, the inner of first breather pipe inserts and cultivates in the bucket and is connected with second breather pipe's upper end, the bottom central point that second breather pipe's lower extreme is located cultivates the bucket puts, install the motor on the bung that cultivates the bucket upper end setting, the bull stick of motor lower extreme output shaft connection inserts and cultivates in the bucket, a plurality of churns the hole has been seted up on the lower extreme demountable installation's of bull stick stirs the board, from last a plurality of stirring vane of installing down on the bull stick, compared with prior art, the beneficial effects of the utility model are that: when bacillus is cultured, airflow is guided into the bottom of the culture barrel through the circular pipe, the second vent pipe and the first vent pipe, and the airflow is ensured to be introduced from the bottom of the culture solution; the motor drives the rotating rod and the stirring plate to rotate, so that bubbles can be broken, the culture solution is fully mixed, and the fermentation effect is improved.

Description

High-efficient culture apparatus of bacillus

Technical Field

The utility model relates to a bacillus cultivates technical field, specifically is a high-efficient culture apparatus of bacillus.

Background

The bacillus belongs to the family of bacillaceae and the genus bacillus, and is a gram-positive bacterium capable of producing resistant endospore, the cell is rod-shaped, and the outer layer is covered with a large amount of calcium dipicolinate. The spores have a thick multi-layer structure with low water content, so the spores have strong refractivity, good stability, oxidation resistance, extrusion resistance and high temperature resistance, can tolerate the high temperature of 60 ℃ for a long time, can survive for 20min at the temperature of 120 ℃, are acid-base resistant, can keep the activity in a gastric acid environment, and are mostly applied in the prior art by bacillus subtilis, bacillus licheniformis, bacillus cereus and the like.

In the process of culturing bacillus, generally, the culture fermentation liquor needs to be stirred and aerated, the culture effect is improved, the aeration quantity is adjusted along with the change of the fermentation time during aeration, and fermentation substances such as a culture medium and the like also need to be added in time in the fermentation process. Experimental research proves that in the bacillus culture process, the fermentation effect can be further improved by introducing airflow to the bottom of a culture container, and in order to ensure the sterile environment of a laboratory, the airflow discharged from the culture device is generally treated to ensure the sterility of the laboratory when bacillus is fermented in the laboratory, so that the efficient culture device for bacillus is provided.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a high-efficient culture apparatus of bacillus to solve the problem that proposes in the above-mentioned background art.

A high-efficiency bacillus culture device comprises a culture barrel, wherein a barrel cover is installed at the upper end of the culture barrel, an annular pipe is arranged outside the upper end of the culture barrel, four first vent pipes are symmetrically connected to the annular pipe, the inner ends of the first vent pipes are inserted into the culture barrel, the inner ends of the first vent pipes are connected with the upper end of a second vent pipe through pipe joints, the pipe joints are screwed on the first vent pipes, so that the first vent pipes are fixedly connected with the second vent pipes, the second vent pipes are bent, the lower ends of the second vent pipes are located at the center of the bottom in the culture barrel, and connecting pipes are further installed on the annular pipe and used for internally connecting vent flows to the annular pipe;

a motor is mounted at the upper end of the barrel cover, a rotating rod connected with an output shaft at the lower end of the motor is inserted into the culture barrel, a stirring plate is detachably mounted at the lower end of the rotating rod, a plurality of stirring holes are formed in the stirring plate, a plurality of stirring blades are mounted on the rotating rod from top to bottom, a plurality of through holes are formed in the stirring blades, a feeding pipe is mounted on the barrel cover and used for adding a culture medium during culture and fermentation, and a pipe cover is detachably mounted at the upper end of the feeding pipe;

still install first blast pipe one end on the bung, be provided with the check valve in the first blast pipe, the air current in the check valve can ensure first blast pipe can only be followed supreme flow down, the first blast pipe other end inserts in the drying cabinet, the upper end of drying cabinet is provided with the case lid, be provided with the drier in the drying cabinet, install the second blast pipe on the case lid.

Preferably, a material discharging pipe is arranged on the side face of the lower end of the culture bucket, a switch valve is arranged on the material discharging pipe, and the culture solution in the culture bucket can be discharged from the material discharging pipe.

Preferably, the connecting rod is installed at the center of the upper end of the flow stirring plate, and the connecting rod is installed at the lower end of the rotating rod through a connecting screw, so that the flow stirring plate can be conveniently disassembled and assembled at the lower end of the rotating rod.

Preferably, the lower extreme of cultivateing the bucket is provided with the heelpiece, and the heelpiece is the rubber material, can play the effect of buffering vibration and pressure.

Compared with the prior art, the beneficial effects of the utility model are that:

when bacillus is cultured, the bacillus culture solution is poured into the culture barrel, the connecting pipe is connected with the air source, air flow enters the circular pipe through the connecting pipe, and finally the air flow is guided into the bottom of the culture barrel through the second vent pipe and the first vent pipe, so that the air flow is ensured to be introduced from the bottom of the culture solution; then the motor is connected with a power supply, the motor drives the rotating rod and the stirring plate to rotate, and the stirring hole formed in the stirring plate can break bubbles generated by introducing airflow into the culture barrel, so that the culture solution is fully contacted with the airflow, and the fermentation effect is improved; the stirring blades are driven to rotate when the rotating rod rotates, the culture solution can be fully mixed through full stirring of the stirring blades and the through holes, and the culture effect is improved;

the air flow in the culture barrel is exhausted into the drying box through the first exhaust pipe, the culture solution contained in the air flow is absorbed by the drying agent in the drying box, so that the bacillus in the culture solution is left in the culture barrel, and the air flow with high purity is exhausted from the second exhaust pipe.

Drawings

Fig. 1 is a front view of the present invention;

fig. 2 is a front view structural section view of the present invention.

In the figure: 1. the culture barrel comprises a culture barrel body, 2, a barrel cover, 3, a first vent pipe, 4, a second vent pipe, 5, a pipe joint, 6, a circular pipe, 7, a connecting pipe, 8, a motor, 9, a rotating rod, 10, a connecting screw, 11, a connecting rod 12, a stirring plate, 121, a stirring hole, 13, a stirring blade, 131, a through hole, 14, a feeding pipe, 15, a pipe cover, 16, a first vent pipe, 17, a one-way valve, 18, a drying box, 19, a box cover, 20, a drying agent, 21, a second vent pipe, 22, a vent pipe, 23, a switch valve, 24 and a bottom pad.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-2, a high-efficiency bacillus culture device comprises a culture barrel 1, wherein a bottom pad 24 is arranged at the lower end of the culture barrel 1, and the bottom pad 24 is made of rubber and can play a role in buffering vibration and pressure; the upper end of the culture barrel 1 is provided with a barrel cover 2, the side surface of the lower end of the culture barrel 1 is provided with a discharge pipe 22, the discharge pipe 22 is provided with a switch valve 23, the upper end of the culture barrel 1 is externally provided with an annular pipe 6, the annular pipe 6 is symmetrically connected with four first vent pipes 3, the inner ends of the first vent pipes 3 are inserted into the culture barrel 1, the inner ends of the first vent pipes 3 are connected with the upper end of a second vent pipe 4 through a pipe joint 5, the lower end of the second vent pipe 4 is positioned at the central position of the bottom in the culture barrel 1, and the annular pipe 6 is also provided with a connecting pipe 7; when the bacillus is cultured, the bacillus culture solution is poured into the culture barrel 1, the connecting pipe 7 is connected with an air source, air flow enters the circular pipe 6 through the connecting pipe 7, and finally the air flow is guided into the bottom of the culture barrel 1 through the second vent pipe 4 and the first vent pipe 3, so that the air flow is ensured to be introduced from the bottom of the culture solution.

A motor 8 is installed at the upper end of the barrel cover 2, a rotating rod 9 connected with an output shaft at the lower end of the motor 8 is inserted into the culture barrel 1, an agitating plate 12 is detachably installed at the lower end of the rotating rod 9, a connecting rod 11 is installed at the center of the upper end of the agitating plate 12, the connecting rod 11 is installed at the lower end of the rotating rod 9 through a connecting screw 10, a plurality of agitating holes 121 are formed in the agitating plate 12, a plurality of agitating blades 13 are installed on the rotating rod 9 from top to bottom, a plurality of through holes 131 are formed in the agitating blades 13, a feeding pipe 14 is installed on the barrel cover 2, and a pipe cover 15 is detachably installed at the upper end of the feeding pipe 14; then the motor 8 is connected with a power supply, the motor 8 drives the rotating rod 9 and the stirring plate 12 to rotate, and the stirring holes 121 formed in the stirring plate 12 can break bubbles generated by introducing airflow into the culture barrel 1, so that the culture solution is fully contacted with the airflow, and the fermentation effect is improved; and drive stirring vane 13 when bull stick 9 rotates and rotate, stirring vane 13 and through-hole 131's intensive mixing can make the culture solution intensive mixing, promote the cultivation effect.

The barrel cover 2 is further provided with one end of a first exhaust pipe 16, a one-way valve 17 is arranged in the first exhaust pipe 16, the other end of the first exhaust pipe 16 is inserted into a drying box 18, a box cover 19 is arranged at the upper end of the drying box 18, a drying agent 20 is arranged in the drying box 18, and a second exhaust pipe 21 is arranged on the box cover 19; the air flow in the culture tank 1 is discharged into the drying box 18 through the first exhaust pipe 16, and the culture solution contained in the air flow is absorbed by the drying agent 20 in the drying box 18, so that the bacillus in the culture solution is left in the culture tank 1, and the air flow with high purity is discharged from the second exhaust pipe 21.

The working principle is as follows: when bacillus is cultured, the bacillus culture solution is poured into the culture barrel 1, the connecting pipe 7 is connected with an air source, air flow enters the circular pipe 6 through the connecting pipe 7, and finally is guided into the bottom of the culture barrel 1 through the second vent pipe 4 and the first vent pipe 3, so that the air flow is ensured to be introduced from the bottom of the culture solution; then the motor 8 is connected with a power supply, the motor 8 drives the rotating rod 9 and the stirring plate 12 to rotate, and the stirring holes 121 formed in the stirring plate 12 can break bubbles generated by introducing airflow into the culture barrel 1, so that the culture solution is fully contacted with the airflow, and the fermentation effect is improved; when the rotating rod 9 rotates, the stirring blades 13 are driven to rotate, the culture solution can be fully mixed through full stirring of the stirring blades 13 and the through holes 131, and the culture effect is improved;

the air flow in the culture tank 1 is discharged into the drying box 18 through the first exhaust pipe 16, and the culture solution contained in the air flow is absorbed by the drying agent 20 in the drying box 18, so that the bacillus in the culture solution is left in the culture tank 1, and the air flow with high purity is discharged from the second exhaust pipe 21.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The utility model provides a high-efficient culture apparatus of bacillus, includes cultivates bucket (1), its characterized in that: the upper end of the culture barrel (1) is provided with a barrel cover (2), the upper end of the culture barrel (1) is externally provided with an annular pipe (6), the annular pipe (6) is symmetrically connected with four first vent pipes (3), the inner ends of the first vent pipes (3) are inserted into the culture barrel (1), the inner ends of the first vent pipes (3) are connected with the upper end of a second vent pipe (4) through a pipe joint (5), the lower ends of the second vent pipes (4) are positioned at the center of the bottom in the culture barrel (1), and the annular pipe (6) is further provided with a connecting pipe (7);

a motor (8) is installed at the upper end of the barrel cover (2), a rotating rod (9) connected with an output shaft at the lower end of the motor (8) is inserted into the culture barrel (1), a stirring plate (12) is detachably installed at the lower end of the rotating rod (9), a plurality of stirring holes (121) are formed in the stirring plate (12), a plurality of stirring blades (13) are installed on the rotating rod (9) from top to bottom, a plurality of through holes (131) are formed in the stirring blades (13), a feeding pipe (14) is installed on the barrel cover (2), and a pipe cover (15) is detachably installed at the upper end of the feeding pipe (14);

the drying device is characterized in that one end of a first exhaust pipe (16) is further mounted on the barrel cover (2), a one-way valve (17) is arranged in the first exhaust pipe (16), the other end of the first exhaust pipe (16) is inserted into the drying box (18), a box cover (19) is arranged at the upper end of the drying box (18), a drying agent (20) is arranged in the drying box (18), and a second exhaust pipe (21) is mounted on the box cover (19).

2. The efficient culture device for bacillus according to claim 1, wherein: the side face of the lower end of the culture barrel (1) is provided with a discharge pipe (22), and the discharge pipe (22) is provided with a switch valve (23).

3. The efficient culture device for bacillus according to claim 1, wherein: the upper end center of the flow stirring plate (12) is provided with a connecting rod (11), and the connecting rod (11) is arranged at the lower end of the rotating rod (9) through a connecting screw (10).

4. The efficient culture apparatus for bacillus according to claim 1 or 2, wherein: the lower end of the culture barrel (1) is provided with a bottom pad (24).