JPS63251077A - Culture device using axial-flow impeller - Google Patents

Culture device using axial-flow impeller

Info

Publication number
JPS63251077A
JPS63251077A JP8490187A JP8490187A JPS63251077A JP S63251077 A JPS63251077 A JP S63251077A JP 8490187 A JP8490187 A JP 8490187A JP 8490187 A JP8490187 A JP 8490187A JP S63251077 A JPS63251077 A JP S63251077A
Authority
JP
Japan
Prior art keywords
cylinder
culture
culture solution
axial flow
flow impeller
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP8490187A
Other languages
Japanese (ja)
Inventor
Masanori Aoki
正則 青木
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ebara Research Co Ltd
Original Assignee
Ebara Research Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ebara Research Co Ltd filed Critical Ebara Research Co Ltd
Priority to JP8490187A priority Critical patent/JPS63251077A/en
Publication of JPS63251077A publication Critical patent/JPS63251077A/en
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • C12M29/08Air lift

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  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PURPOSE:To efficiently cultivate cells, etc., by reduced power, by setting an axial-flow impeller in a cylinder in a culture tank, rotating the impeller, making a culture solution, cells, etc., flow from the top of the cylinder on liquid surface of the culture solution outside the cylinder. CONSTITUTION:A cylinder 13 is set in a culture tank 11 in such a way that the top of the cylinder is protruded from liquid surface W of a culture solution and an axial-flow impeller 15 is laid in the cylinder 13. The impeller 15 is rotated, the culture solution (l), cells (c), etc., are made to flow from the top of the cylinder 13 on the liquid surface W of culture solution outside the cylinder 13. Consequently, an oxygen-containing gas in the upper part of the culture tank 11 is entangled in the culture solution (l) to feed oxygen to the culture solution (l) and to stir the culture solution (l), the cells (c), etc. Consequently, since stirring in the culture tank and feed of oxygen can be simultaneously carried out only by rotating the axial-flow impeller, the cells, etc., can be cultivated efficiently and simply by less power.

Description

【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、動植物の細胞や菌などの微生物を培養するた
めの装置に関し、細胞等が雑菌におかされることなく、
少ない動力で細胞等に酸素供給すると共に1培養液を攪
拌して代謝生産物の物質移動を促進することができるよ
うにした培養装置に関する。
[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to an apparatus for culturing microorganisms such as animal and plant cells and bacteria, and a device for culturing microorganisms such as animal and plant cells and bacteria.
The present invention relates to a culture device capable of supplying oxygen to cells, etc. with a small amount of power, and stirring a single culture solution to promote mass transfer of metabolic products.

〔従来の技術及び問題点〕[Conventional technology and problems]

一般に、動植物の細胞や菌などの微生物を培養するため
には、培養液を介して細胞等に酸素を供給すると共に、
培養液と細胞等を混合攪拌して代謝生産物の物質移動を
促進する必要がある。
Generally, in order to culture animal and plant cells and microorganisms such as bacteria, oxygen is supplied to the cells etc. through a culture solution, and
It is necessary to mix and stir the culture solution and cells to promote mass transfer of metabolic products.

従来は、例えば第7図に示すような培養装置を設け、散
気管1から気泡すを培養液を中に放出すると共に、攪拌
機2によって該培養atを攪拌・混合していた。なお、
図中、3は培養槽(容器)、4は債拌機2の攪拌効果を
高めるために内壁面に突設され九邪魔板、gは散気管1
へ送り込む空気である。
Conventionally, for example, a culture apparatus as shown in FIG. 7 was provided, and bubbles were discharged into the culture solution from an aeration pipe 1, and the culture solution was stirred and mixed by a stirrer 2. In addition,
In the figure, 3 is a culture tank (container), 4 is a baffle plate protruding from the inner wall surface to enhance the stirring effect of the agitator 2, and g is a diffuser pipe 1.
This is the air that is sent to the

しかしながら、上記従来の装置では、培養槽3へ外部の
空気を圧入するため、外部空気の雑菌が培養槽内に混入
しないよって除菌フィルタの使用が不可欠である。しか
し、除菌フィルタは極めて微細な間隙を有する多孔体で
あるので、その過大な流動抵抗と培養液の水圧に打ち勝
って大量の空気と通過させるには、多大の動力が必要と
なり、ま念、攪拌の念めの羽根車にも別途動力が必要で
あるという問題点があった。
However, in the conventional apparatus described above, since external air is forced into the culture tank 3, it is essential to use a sterilization filter to prevent bacteria from the external air from entering the culture tank. However, since the sterilization filter is a porous material with extremely fine gaps, a large amount of power is required to overcome the excessive flow resistance and water pressure of the culture solution and pass a large amount of air. There was a problem in that the impeller for stirring also required separate power.

また、第8図に示すように、培養槽3の下方より空気g
を送り込んで槽内で気泡すを発生させ、該気泡すだけに
よって培養液tを流動攪拌させるようにした培養装置で
は、培養が進んで培養atの粘度が大きくなると、十分
な攪拌効果を得ることができず、空気を圧送するだめの
動力は更に過大になるという問題点があった。
In addition, as shown in FIG. 8, air g is added from below the culture tank 3.
In a culture device in which air bubbles are generated in the tank by sending the air bubbles into the tank, and the culture solution T is fluidized and stirred only by the bubbles, as the culture progresses and the viscosity of the culture at increases, a sufficient stirring effect cannot be obtained. However, there was a problem in that the power required to pump the air was even more excessive.

本発明は、動植物の細胞や菌を、雑菌で汚染せずにより
少ない動力で攪拌しながらR素供給することができる培
養装置を提供することを技術的鯉題としている。
The technical objective of the present invention is to provide a culture device that can supply R element to animal and plant cells and bacteria while stirring them with less power without contaminating them with germs.

〔問題点を解決するための手段〕[Means for solving problems]

本発明は、上記した従来技術の問題点及び技術的裸題を
解決するために、培養槽の内部に円筒をその上部を培養
液面より突出させるようにして設け、該円筒内部に軸流
羽根車を設け、該軸流羽根車を回転させることにより、
培養液と細胞等を円筒上部から流出させて円筒外部の培
養液面上に落下させるようにし、これによって培養槽内
上部の酸素含有気体を培養液内に巻き込んで培養液圧酸
素を供給すると共に、培養液と細胞等を攪拌するように
したことを特徴としている。
In order to solve the problems and technical problems of the prior art described above, the present invention provides a cylinder inside a culture tank with its upper part protruding from the culture solution surface, and an axial flow impeller inside the cylinder. By providing a wheel and rotating the axial flow impeller,
The culture solution and cells, etc. are allowed to flow out from the top of the cylinder and fall onto the surface of the culture solution outside the cylinder, thereby entraining the oxygen-containing gas at the top of the culture tank into the culture solution to supply culture solution pressure oxygen. , it is characterized by stirring the culture solution, cells, etc.

なお、上記円筒は、固定しておいてもよく、また軸流羽
根車と共に回転させるようにしてもよい。
Note that the cylinder may be fixed or may be rotated together with the axial flow impeller.

〔作用〕[Effect]

本発明は上記のように構成されているので、培養槽内に
培養液を所定レベルまで入れると、該培養液は、上部を
培養液面上に突出するようにして設けられた円筒の下端
開口部より進入して、該円筒の内外でほぼ同一レベルに
達する。次いで、この状態で軸流羽根車を回転させると
、培養液細胞等は、円筒内部を軸流羽根車の揚液作用に
よって上昇し、該円筒上部の開口部から円筒外部へ溢れ
出し、培養液面上に落下する。
Since the present invention is configured as described above, when the culture solution is poured into the culture tank to a predetermined level, the culture solution is absorbed into the culture tank through the lower end opening of the cylinder provided with the upper part protruding above the culture solution surface. The inside and outside of the cylinder reach approximately the same level. Next, when the axial flow impeller is rotated in this state, the culture solution cells, etc. rise inside the cylinder due to the liquid lifting action of the axial flow impeller, overflow from the opening at the top of the cylinder to the outside of the cylinder, and the culture solution fall onto the surface.

上記のように、培養液細胞等が円筒上部の開口部から培
養液面上に落下するとき、培養槽内上部の酸素含有気体
を該培fX液内に巻き込んで培養液に酸素が供給される
。そして落下された液は槽内の培養液と混合され、軸流
羽根車の作用で円筒の下端開口部から円筒内へ吸い込ま
れ、円筒内を再び上“昇して上部開口部から流出する。
As mentioned above, when culture solution cells, etc. fall from the opening at the top of the cylinder onto the culture solution surface, the oxygen-containing gas from the upper part of the culture tank is drawn into the culture fX solution, and oxygen is supplied to the culture solution. . The dropped liquid is mixed with the culture liquid in the tank, sucked into the cylinder from the lower end opening by the action of the axial impeller, rises again inside the cylinder, and flows out from the upper opening.

このようにして、円筒の内外で循環流が形成され、その
過程で酸素が供給されると共に、該循環流によって培養
液と細胞等は攪拌・混合される。
In this way, a circulating flow is formed inside and outside the cylinder, and in the process, oxygen is supplied, and the culture solution, cells, etc. are agitated and mixed by the circulating flow.

〔実施例〕〔Example〕

次に、本発明の実施例を図面と共に説明する。 Next, embodiments of the present invention will be described with reference to the drawings.

第1図は本発明の第1実施例を示す細胞等の培養装置の
縦断面図である。
FIG. 1 is a longitudinal sectional view of a cell culturing apparatus showing a first embodiment of the present invention.

図において、培養槽(容器)11の中央付近に蓋12と
一体化された円筒13が培養#J11に固定して取付け
られており、該固定された円筒13の内部に、回転軸1
4に取付けられた軸流羽根車15が設けられている。
In the figure, a cylinder 13 integrated with a lid 12 is fixedly attached to culture #J11 near the center of a culture tank (container) 11, and a rotating shaft 1 is attached inside the fixed cylinder 13.
An axial flow impeller 15 mounted at 4 is provided.

軸流羽根車15は、例えば平板翼を、回転方向(軸に直
角方向)に対して傾斜して植設して構成されている。な
お、該軸流羽根車15を第5図に示すようならせん翼5
5で構成することも可能である。
The axial impeller 15 is configured by, for example, having flat blades installed at an angle with respect to the rotation direction (direction perpendicular to the axis). Note that the axial flow impeller 15 is a spiral blade 5 as shown in FIG.
It is also possible to configure it with 5.

上記固定された円筒13の上部には槽内の液面Wより上
方位置において複数個の流出孔16が穿設されており、
軸流羽根車15の回転に伴って、二重矢印■のように、
揚液された培養液tと細胞C等は、該流出孔16から円
筒13の外へ流出(湿田)シ、培養槽11内の液面W上
に、二重矢印@のように落下する。この落下に伴ない、
培養槽11の液面Wより上部の空気aが気泡すとなって
培養液り内に巻き込まれ、培養液tに酸素が供給される
A plurality of outflow holes 16 are bored in the upper part of the fixed cylinder 13 at positions above the liquid level W in the tank,
As the axial flow impeller 15 rotates, as shown by the double arrow ■,
The pumped culture solution t, cells C, etc. flow out of the cylinder 13 from the outflow hole 16 (wet field) and fall onto the liquid surface W in the culture tank 11 as shown by the double arrow @. Along with this fall,
Air a above the liquid level W of the culture tank 11 becomes bubbles and is drawn into the culture liquid reservoir, and oxygen is supplied to the culture liquid t.

このようにして気泡すを混合した培養液tは、軸流羽根
車15による循環流に従って、二重矢印■のように、円
筒13の下端部と底板12aの彎曲された上面との簡を
経て円筒13の内部へ流入する。
The culture solution t mixed with air bubbles in this way follows the circulation flow by the axial impeller 15 and passes through the lower end of the cylinder 13 and the curved upper surface of the bottom plate 12a, as shown by the double arrow ■. It flows into the inside of the cylinder 13.

一方、培養槽11内は総べて殺菌されており、該培養槽
上部の空気凰も無菌状態である。従って、培養液を中の
細胞C等が雑菌に汚染されることはない。
On the other hand, the entire inside of the culture tank 11 is sterilized, and the air hood above the culture tank is also sterile. Therefore, cells C and the like in the culture solution are not contaminated with germs.

また、円筒上部の流出孔16と培養液の液面Wとの水位
差は小さく、培養液り等を流出孔16から流出させるた
めの軸流羽根車15の回転数は小さくてよいので、該羽
根車15の回転による細胞C等の破損は少ない。
In addition, the water level difference between the outflow hole 16 at the top of the cylinder and the liquid level W of the culture solution is small, and the rotation speed of the axial flow impeller 15 for flowing out the culture solution etc. from the outflow hole 16 may be small. There is little damage to the cells C etc. due to the rotation of the impeller 15.

この実施例によれば、軸流羽根車15の回転だけで培養
槽11内の攪拌と酸素の供給を同時に行なうことができ
るので少ない動力で効率的且つ簡便に細胞等の培養を行
なうことができる。
According to this embodiment, it is possible to simultaneously stir the inside of the culture tank 11 and supply oxygen by simply rotating the axial flow impeller 15, so cells, etc. can be cultured efficiently and easily with less power. .

なお培養液りを円筒13上部の流出孔16から液面W上
に落下させることによる空気aの巻き込みだけでは酸素
の供給量が不足する場合には、軸流?ンプ15の下方に
設けられた散気孔17から殺菌した空気等の酸素含有空
気gを補助的に混入させてもよい。
In addition, if the amount of oxygen supplied is insufficient only by entraining air a by dropping the culture solution from the outflow hole 16 at the top of the cylinder 13 onto the liquid surface W, axial flow? Oxygen-containing air g, such as sterilized air, may be supplementarily mixed in through the air diffuser hole 17 provided below the pump 15.

第2図は、本発明の第2実施例を示す細胞等の培養装置
の縦断面図であって、図中、第1図に記載した符号と同
一の符号は同一ないし同類部分を示すものとする。
FIG. 2 is a longitudinal cross-sectional view of a cell culture device showing a second embodiment of the present invention, and in the figure, the same reference numerals as those shown in FIG. 1 indicate the same or similar parts. do.

この実施例では、上端部に流出孔26を下向きに設けた
円筒23が、支持円板23aを介して回転軸14に取付
けられており、培養槽11の中央に位置する軸方向に長
い軸流羽根車25の外周と一体となって回転するように
なっている。図中、16は上蓋12に設けられた換気孔
、17は該換気孔16の途中に設けられた除菌フィルタ
である。
In this embodiment, a cylinder 23 with an outflow hole 26 facing downward at its upper end is attached to a rotating shaft 14 via a support disk 23a, and an axially long cylinder 23 located at the center of the culture tank 11 It rotates integrally with the outer periphery of the impeller 25. In the figure, 16 is a ventilation hole provided in the upper lid 12, and 17 is a sterilization filter provided in the middle of the ventilation hole 16.

この実施例によれば、円筒23は軸流羽根車25と共に
一体になって回転するので、前記第1実施例(第1図)
のように、細胞C等が、羽根車15の外周面と円筒13
の内面との隙間に挾まれて破壊するようなことはない。
According to this embodiment, the cylinder 23 rotates together with the axial flow impeller 25, so as shown in the first embodiment (FIG. 1)
As shown in FIG.
There is no chance of it getting caught in the gap between the inner surface of the body and destroying it.

また、第1実施例と比較して軸流羽根車25は軸方向に
長いので、より低い回転数で目的を達することができ、
従って細胞等の破損の程度は更に軽減する。
In addition, since the axial flow impeller 25 is longer in the axial direction than in the first embodiment, the purpose can be achieved at a lower rotation speed.
Therefore, the degree of damage to cells etc. is further reduced.

第3図は、本発明のv13実施例を示す要部縦断面図で
あって、図中、第2図に記載した符号と同一の符号は同
一なりし同類部分を示すものとする。
FIG. 3 is a vertical cross-sectional view of a main part showing the v13 embodiment of the present invention, and in the figure, the same reference numerals as those shown in FIG. 2 indicate the same or similar parts.

図において、円筒23が培養槽11のほぼ中央に位置す
る軸方向に長い軸流羽根車25の外周と一体化されて回
転する点は、第2実施例(第2図)と変りはないが、こ
の実施例では、上記回転する円筒23の外側に特に攪拌
羽根35が設けられている。
In the figure, the point that the cylinder 23 rotates integrally with the outer circumference of an axially long axial flow impeller 25 located approximately at the center of the culture tank 11 is the same as in the second embodiment (FIG. 2). In this embodiment, stirring blades 35 are particularly provided on the outside of the rotating cylinder 23.

この実施例によれば、回転軸14の回転により、軸流羽
根車25と円筒23とが一体となって回転すると、培養
槽ll内の液は、2重矢印■○のように円筒23の外側
から内側へ流入する循環流が生じる外円筒23の外側に
設けられた攪拌羽根35により、二重矢印Oのような押
し付は流れや二重矢印■のような旋回流れが生じ、これ
らの流れによって培養槽11の底面附近に滞留し勝ちな
細胞C等を流動させ、効率良く軸流羽根車25に吸い込
ませるようにすることができる。
According to this embodiment, when the axial flow impeller 25 and the cylinder 23 rotate together as a result of the rotation of the rotating shaft 14, the liquid in the culture tank 11 flows into the cylinder 23 as shown by the double arrow ■○. Due to the stirring blades 35 provided on the outside of the outer cylinder 23 where a circulating flow flows from the outside to the inside, a pressing flow as shown by the double arrow O and a swirling flow as shown by the double arrow ■ are generated. Cells C, etc. that tend to stay near the bottom of the culture tank 11 can be caused to flow by the flow, and can be efficiently sucked into the axial flow impeller 25.

第4図は、本発明の第4実施例を示す縦断面図であって
、図中、第2図に記載した符号と同一の符号は同一ない
し同類部分を示すものとする。
FIG. 4 is a longitudinal sectional view showing a fourth embodiment of the present invention, and in the figure, the same reference numerals as those shown in FIG. 2 indicate the same or similar parts.

図において、円筒23が培養槽11のほぼ中央に位置す
る軸方向に長い軸流羽根車25の外周と一体化されて回
転する点は、第2実施例(第2図)と変りはないが、こ
の実施例では特に軸流羽根車25を不動する回転軸44
が、マグネットカッゾリン4P(電磁継手)を介して駆
動されるようになっている。
In the figure, the point that the cylinder 23 rotates integrally with the outer circumference of an axially long axial flow impeller 25 located approximately at the center of the culture tank 11 is the same as in the second embodiment (FIG. 2). In this embodiment, the rotating shaft 44 that keeps the axial flow impeller 25 stationary is used.
is driven via a magnet cassolin 4P (electromagnetic joint).

即ち、培養槽11の底板42の中央部だステンレスその
他の非磁性体からなる薄板(仕切り板)42aが形成さ
れ、該薄板42aと細隙を保って、上側つまり培養槽1
1の内側には、回転軸44に直結された回転円板41が
、その外周面を、前記薄板42aの外周部に形成された
底板42の厚肉凹部42bの内周面に細隙を保って嵌入
するよう込まれている。
That is, a thin plate (partition plate) 42 a made of stainless steel or other non-magnetic material is formed at the center of the bottom plate 42 of the culture tank 11 , and a thin plate 42 a is formed in the center of the bottom plate 42 of the culture tank 11 .
1, a rotating disk 41 directly connected to a rotating shaft 44 maintains a gap between its outer circumferential surface and the inner circumferential surface of a thick recess 42b of the bottom plate 42 formed on the outer circumferential portion of the thin plate 42a. It is inserted so that it can be inserted.

他方、薄板42aの下側、つまり培養槽11の外側には
細隙を保って駆動マグネット43aを埋め込んだ駆動円
板43が対向して設けられており、該駆動円板43は駆
動軸43bを介して駆動源に連結されている。
On the other hand, on the lower side of the thin plate 42a, that is, on the outside of the culture tank 11, a driving disk 43 in which a driving magnet 43a is embedded with a narrow gap is provided to face the driving disk 43, and the driving disk 43 has a driving shaft 43b. The drive source is connected to the drive source via the drive source.

上記の薄板42aを挾み細隙を保って対設された、被駆
動又は駆動各マグネットをそれぞれ埋込んだ回転円板4
1と駆動円板43とによってマグネットカップリングが
形成されている。該マグネットカップリングにおいて、
回転円板41を薄板42Lと細隙を保って回転させるた
めに、該回転円板41ば、薄板42aに植設された固定
軸42cK@合支持されている。
A rotating disk 4 in which each driven or driving magnet is embedded, which is arranged opposite to each other with a gap between the thin plates 42a mentioned above.
1 and the drive disk 43 form a magnetic coupling. In the magnetic coupling,
In order to rotate the rotary disk 41 while maintaining a narrow gap with the thin plate 42L, the rotary disk 41 is supported by a fixed shaft 42cK implanted in the thin plate 42a.

なお、上記のように培養槽内に配はされる回転円板41
を薄板42aと細隙を保って回転させるために、該回転
円板41の薄板42aとの対向面に、回転方向に勤王を
生じる方向に刻設された多数のス・臂イラル溝を形成し
、運転時、W劾円板43の回転につれて磁気的に結合さ
れた該回転円板41が同方向に回転するにつれて、上記
スパイラル溝付き表面と薄板42aとの間に介在する流
体てよって動圧を生じさせ該回転円板41を上記動圧を
生じている圧力流体膜によって浮上させて推力支持させ
るようにすることも可能である。図中、45は回転円u
i41の上面に設けられた攪拌羽根である。
In addition, as mentioned above, the rotating disk 41 arranged in the culture tank
In order to rotate the rotary disc 41 while maintaining a narrow gap with the thin plate 42a, a large number of radial grooves are formed on the surface of the rotating disk 41 facing the thin plate 42a, which are engraved in a direction that causes rotation in the direction of rotation. During operation, as the magnetically coupled rotating disk 41 rotates in the same direction as the W-shaped disk 43 rotates, dynamic pressure is generated by the fluid interposed between the spiral grooved surface and the thin plate 42a. It is also possible to cause the rotating disk 41 to float and support thrust by a pressure fluid film that generates the dynamic pressure. In the figure, 45 is the rotation circle u
This is a stirring blade provided on the top surface of i41.

この実施例によれば、軸流羽根車25はマグネットカッ
プリングを介して1駆動され、回転軸44は培養槽11
の底板42を貫通してhないので軸封部が不用となる。
According to this embodiment, the axial flow impeller 25 is driven via a magnetic coupling, and the rotating shaft 44 is connected to the culture tank 11.
Since the shaft does not penetrate through the bottom plate 42 of the shaft, a shaft seal is unnecessary.

従って、軸封部から培養槽ll内に雑菌が侵入して細胞
C等が汚染する恐れがなく゛ま九、回転円板41の上面
に設けられた攪拌羽根45の回転により、二重矢印■に
示すような流れが生じ、これによって培養槽11の底部
で細胞C等が滞留する恐れもない。
Therefore, there is no risk of bacteria entering into the culture tank 11 from the shaft sealing part and contaminating the cells C, etc. By the rotation of the stirring blade 45 provided on the upper surface of the rotating disk 41, A flow as shown is generated, and there is no fear that the cells C and the like will stay at the bottom of the culture tank 11.

第6図は、本発明の培養装置の一使用例を示す縦断面図
であって、図中、第2図に記載した符号と同一の符号は
同一ないし同類部分を示すものとする。
FIG. 6 is a longitudinal sectional view showing an example of the use of the culture device of the present invention, and in the figure, the same reference numerals as those shown in FIG. 2 indicate the same or similar parts.

この使用例では、大きな培養槽61内に複数個(図では
3個)の円筒23と一体に回転する軸流羽根車25が回
転軸14によって駆動するように配設されている。
In this usage example, an axial flow impeller 25 that rotates together with a plurality of cylinders 23 (three in the figure) is arranged in a large culture tank 61 so as to be driven by the rotating shaft 14.

この使用例によれば、複数個の軸流羽根車O働きにより
、大きな培養槽における培養液への酸素供給及び培養液
と細胞等の攪拌作用が有効に行われる。
According to this example of use, the action of the plurality of axial flow impellers effectively supplies oxygen to the culture solution in a large culture tank and agitates the culture solution, cells, etc.

なお、上記使用例では、第2実施列(第2図)に示すよ
うな軸流羽根車と円筒とを一体に回転するようにした形
式のものが使用されているが、上記したその他の実施例
に記載した形式の培養装置を複数個(台)併設して使用
することも勿論可能である。
In the above usage example, an axial flow impeller and a cylinder that rotate together as shown in the second implementation row (Fig. 2) are used, but other implementations described above are also used. Of course, it is also possible to use a plurality of culture devices (units) of the type described in the example in parallel.

〔発明の効果〕〔Effect of the invention〕

以上説明したように本発明によれば培養槽の内部に円筒
を設けると共に該円筒内部に軸流羽根車を設け、該羽根
車を回転することにより、培養液と細胞等を円筒上部か
ら流出させて円筒外部の培養液面上に落下させるように
したことにより、軸流羽根車の回転だけで培養槽内の攪
拌と酸素の供給を同時に行なうことができるので、少な
い動力で、効率的且つ情便に細胞等の培養を行なうこと
ができる。
As explained above, according to the present invention, a cylinder is provided inside the culture tank, and an axial flow impeller is provided inside the cylinder, and by rotating the impeller, the culture solution, cells, etc. are caused to flow out from the top of the cylinder. By allowing the liquid to fall onto the surface of the culture liquid outside the cylinder, it is possible to stir the culture tank and supply oxygen at the same time just by rotating the axial flow impeller, which is efficient and informative with less power. Cells, etc. can be cultured in stool.

【図面の簡単な説明】[Brief explanation of the drawing]

第1図ないし第4図は本発明の第1ないし第4の各実施
例を示す培養装置の縦断面図、第5図は要部断面図、第
6図は本発明の使用例を示す縦断面図、第7図及び第8
図は従来例を示す説明図である。 11・・・培養槽、  13.23・・・円筒、  1
4゜44・・・回転軸、  15.25.55・・・軸
流羽根車、  16.26・・・流出孔、  35.4
5・・・攪拌羽根−a・・・空気、  b・・・気泡、
  C・・・細胞、g・・・空気、 t・・・培養液。 第1図 第5図 第6図
1 to 4 are longitudinal cross-sectional views of a culture device showing the first to fourth embodiments of the present invention, FIG. 5 is a cross-sectional view of main parts, and FIG. 6 is a longitudinal cross-sectional view showing an example of use of the present invention. Front view, Figures 7 and 8
The figure is an explanatory diagram showing a conventional example. 11...Culture tank, 13.23...Cylinder, 1
4゜44... Rotating shaft, 15.25.55... Axial flow impeller, 16.26... Outflow hole, 35.4
5... Stirring blade - a... Air, b... Bubbles,
C...Cells, g...Air, t...Culture solution. Figure 1 Figure 5 Figure 6

Claims (1)

【特許請求の範囲】 1、培養槽の内部に円筒をその上部を培養液面より突出
させるようにして設けると共に、該円筒内部に軸流羽根
車を設け、該羽根車を回転することにより、培養液と細
胞等を円筒上部から流出させて円筒外部の培養液面上に
落下させるようにし、これによって培養槽内上部の酸素
含有気体を培養液内に巻き込んで該培養液に酸素を供給
すると共に培養液と細胞等を攪拌するようにしたことを
特徴とする軸流羽根車を用いた培養装置。 2、上記円筒が固定されている特許請求の範囲第1項記
載の軸流羽根車を用いた培養装置。 3、上記円筒が軸流羽根車の外周と一体化され、該羽根
車と共に回転されるようになっている特許請求の範囲第
1項記載の軸流羽根車を用いた培養装置。 4、上記の軸流羽根車と共に回転する円筒の外側に攪拌
手段が設けられている特許請求の範囲第3項記載の軸流
羽根車を用いた培養装置。 5、上記の軸流羽根車が、該軸流羽根車の下方に設けた
マグネットカップリングで駆動されるようになっている
特許請求の範囲第1項ないし第3項の何れか1項記載の
軸流羽根車を用いた培養装置。 6、上記マグネットカップリングの被駆動マグネットを
覆う回転円板上に攪拌手段が設けられている特許請求の
範囲第5項記載の軸流羽根車を用いた培養装置。
[Claims] 1. A cylinder is provided inside the culture tank with its upper part protruding from the surface of the culture solution, an axial flow impeller is provided inside the cylinder, and the impeller is rotated. The culture solution and cells, etc. are allowed to flow out from the top of the cylinder and fall onto the surface of the culture solution outside the cylinder, thereby drawing the oxygen-containing gas from the top of the culture tank into the culture solution and supplying oxygen to the culture solution. A culture device using an axial flow impeller, characterized in that the culture solution, cells, etc. are agitated at the same time. 2. A culture device using an axial flow impeller according to claim 1, wherein the cylinder is fixed. 3. A culture device using an axial flow impeller according to claim 1, wherein the cylinder is integrated with the outer periphery of the axial flow impeller and rotated together with the impeller. 4. A culture apparatus using an axial flow impeller according to claim 3, wherein stirring means is provided on the outside of the cylinder that rotates together with the axial flow impeller. 5. The axial flow impeller described in any one of claims 1 to 3, wherein the axial flow impeller is driven by a magnetic coupling provided below the axial flow impeller. A culture device using an axial flow impeller. 6. A culture device using an axial flow impeller according to claim 5, wherein stirring means is provided on a rotating disk that covers the driven magnet of the magnetic coupling.
JP8490187A 1987-04-08 1987-04-08 Culture device using axial-flow impeller Pending JPS63251077A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP8490187A JPS63251077A (en) 1987-04-08 1987-04-08 Culture device using axial-flow impeller

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP8490187A JPS63251077A (en) 1987-04-08 1987-04-08 Culture device using axial-flow impeller

Publications (1)

Publication Number Publication Date
JPS63251077A true JPS63251077A (en) 1988-10-18

Family

ID=13843638

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8490187A Pending JPS63251077A (en) 1987-04-08 1987-04-08 Culture device using axial-flow impeller

Country Status (1)

Country Link
JP (1) JPS63251077A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0424444U (en) * 1990-06-20 1992-02-27
JPH06327460A (en) * 1993-05-21 1994-11-29 Kirin Brewery Co Ltd Air lift-type reactor for feeding culture
JPH08322549A (en) * 1995-05-31 1996-12-10 Masatoshi Matsumura Liquid flow-type biochemical reaction apparatus and purifying system of ground water or drain using the same apparatus
KR101970135B1 (en) * 2018-10-18 2019-04-18 한국해양과학기술원 ROSE Max Continuous Circulated Bioreactor

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0424444U (en) * 1990-06-20 1992-02-27
JP2523276Y2 (en) * 1990-06-20 1997-01-22 祐也 鴨下 Aquatic creature egg hatching device
JPH06327460A (en) * 1993-05-21 1994-11-29 Kirin Brewery Co Ltd Air lift-type reactor for feeding culture
JPH08322549A (en) * 1995-05-31 1996-12-10 Masatoshi Matsumura Liquid flow-type biochemical reaction apparatus and purifying system of ground water or drain using the same apparatus
KR101970135B1 (en) * 2018-10-18 2019-04-18 한국해양과학기술원 ROSE Max Continuous Circulated Bioreactor

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