CN114480121A - Reaction device suitable for cell growth - Google Patents

Reaction device suitable for cell growth Download PDF

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Publication number
CN114480121A
CN114480121A CN202210034494.XA CN202210034494A CN114480121A CN 114480121 A CN114480121 A CN 114480121A CN 202210034494 A CN202210034494 A CN 202210034494A CN 114480121 A CN114480121 A CN 114480121A
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China
Prior art keywords
sliding rod
reactor
stirring disc
sliding
cell growth
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CN202210034494.XA
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Chinese (zh)
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CN114480121B (en
Inventor
张百峰
段瑞雪
韩伟
朱继玲
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Shandong Shuifa Life Science Research Co ltd
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Shandong Shuifa Life Science Research Co ltd
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Publication of CN114480121A publication Critical patent/CN114480121A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/07—Means for pre-treatment of biological substances by electrical or electromagnetic forces

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Electromagnetism (AREA)
  • Molecular Biology (AREA)
  • Thermal Sciences (AREA)
  • Computer Hardware Design (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a reaction vessel, in particular to a reaction device suitable for cell growth, which comprises a reactor made of stainless steel, wherein a bracket is fixed at the bottom of the reactor, a vertical sliding rod positioned in the middle of the reactor is fixed on the bracket, a disc-shaped stirring disc capable of moving up and down on the sliding rod is arranged on the sliding rod, a sliding hole with the diameter slightly larger than that of the sliding rod is arranged in the center of the stirring disc, the sliding rod and the bracket are made of stainless steel materials, and the stirring disc is made of iron materials; an electromagnet capable of magnetically attracting the stirring disc is arranged above the reactor, a stop switch for stopping the electromagnet is arranged at the upper end of the sliding rod, and an operation switch for operating the electromagnet is arranged at the lower end of the sliding rod. The invention has the advantages of good mixing effect and avoiding damaging cells; can not cause cell pollution.

Description

Reaction device suitable for cell growth
Technical Field
The invention relates to a reaction vessel, in particular to a reaction device suitable for cell growth. .
Background
The reaction conditions required by the cell culture reaction are very strict, and the requirement on environmental cleanliness is high. In addition, the cellular reaction is different from other chemistry, namely, the culture solution is required to have certain fluidity and cannot be too violent, so that the cells are prevented from being damaged.
At present, in order to fully mix cells and nutrient substances, anchor-type stirring reaction kettles are adopted in reaction containers for cell growth, and the reaction kettles have the defects that firstly, the stirring speed is high, the cells are easily damaged, secondly, the stirring effect is poor, and the culture solution can not be exchanged up and down because the liquid only moves in the circumferential direction. And thirdly, lubricating oil and a packing for sealing are needed for sealing above the stirring shaft, so that the cells are easily polluted and the growth of the cells is influenced.
Disclosure of Invention
In order to overcome the defects, the invention provides a reaction device suitable for cell growth, and overcomes the defects of high stirring speed, poor stirring effect and reaction environment pollution.
The invention is realized by the following measures:
the utility model provides a reaction unit suitable for cell growth, includes the reactor of being made by the stainless steel, is provided with heating device, discharge gate, feed inlet, detection mouth on the reactor, its characterized in that: a support is fixed at the bottom of the reactor, a vertical sliding rod positioned in the middle of the reactor is fixed on the support, a disc-shaped stirring disc capable of moving up and down on the sliding rod is arranged on the sliding rod, a sliding hole with the diameter slightly larger than that of the sliding rod is arranged in the center of the stirring disc, the sliding rod and the support are made of stainless steel materials, and the stirring disc is made of iron materials; an electromagnet capable of magnetically attracting the stirring disc is arranged above the reactor, a stop switch for stopping the electromagnet is arranged at the upper end of the sliding rod, and an operation switch for operating the electromagnet is arranged at the lower end of the sliding rod.
In the above reaction apparatus for cell growth, it is preferable that the stirring plate is coated with a plastic material on the outer circumference thereof in order to prevent rusting and maintain the cleanness of the inside of the reactor.
In the above reaction apparatus for cell growth, preferably, in order to prevent turbulence of the liquid and keep the stirring plate stable, the stirring plate is provided with four circulation holes which are circumferentially and uniformly distributed and have the same diameter.
The reaction device suitable for cell growth described above is preferably: the height of the sliding rod is 0.5-1 times of the height of the reactor, and the diameter of the stirring disc is 0.2-0.5 times of the diameter of the reactor.
Preferably, in order to ensure the stable ascending and descending of the stirring disc, the sliding rods are three or four in parallel, and the stirring disc is provided with three or four sliding holes matched with the sliding rods.
The stirring disc moves upwards along the sliding rod through the attraction of the electromagnet, stops moving when the stirring disc moves to the stop switch at the upper end, moves downwards by means of gravity, and moves upwards next time when the stirring disc slides to the lower end of the sliding rod to operate the switch, and the stirring disc circularly reciprocates.
Because the stirring disc is a horizontal disc, when the stirring disc moves upwards, the reaction liquid above the stirring disc can be driven to move upwards, the reaction liquid around the stirring disc is driven to move downwards, and the reaction liquid at the bottom of the reactor moves upwards, so that the up-and-down exchange of the reaction liquid is completed. When the stirring plate is operated downward, the reaction liquid is operated in reverse. The reaction liquid can be operated up and down once in each operation period of the stirring disc, so that the reaction liquid can be well mixed. The stirring disc of the invention can achieve good effect under the condition of slow running.
Four circulation holes are formed in the middle of the stirring disc, so that part of reaction liquid can pass through the circulation holes, and the stirring disc can be guaranteed to stably run up and down. The turbulence of the reaction liquid is prevented, and the stirring plate is kept as horizontal as possible.
The sliding rods are three or four in parallel, so that the stirring disc can be kept horizontal better, and deviation is prevented.
The invention has the advantages that through the up-and-down operation of the stirring disc, a better stirring effect is obtained under the condition of slow speed, so that the up-and-down materials are mixed more quickly, and the damage to cells is avoided; because the stirring disc and the sliding rod position reaction device are arranged inside, the sealing of the stirring shaft is not needed, the pollution of lubricating oil to cells is avoided, and the growth of the cells is ensured.
Drawings
FIG. 1 is a schematic view of the structure of a reaction apparatus according to example 1 of the present invention
FIG. 2 is a schematic top view of the stirring plate of embodiment 1 of the present invention
FIG. 3 is a schematic top view of the stirring plate of embodiment 2 of the present invention
In the figure, 1 detection port, 2 feeding ports, 3 discharging ports, 4 heating devices, 5 supports, 6 stirring discs, 7 sliding rods, 8 running switches, 9 stop switches, 10 sliding holes, 11 circulating holes and 12 electromagnets are arranged.
Example 1
The reaction device suitable for cell growth of the invention, as shown in figure 1, comprises a reactor made of stainless steel, and the reactor is provided with a heating device 4, a discharge port 3, a feed port 2 and a detection port 1. The bottom of the reactor is fixed with a bracket 5 which is crossed and fixed on the inner wall of the reactor. A vertical sliding rod 7 positioned in the middle of the reactor is fixed on the bracket 5, the lower end of the sliding rod is fixed on the bracket, and the upper end of the sliding rod is fixed on the inner wall of the upper end of the reactor. The sliding rod 7 is provided with a disc-shaped stirring disc 6 which can move up and down on the sliding rod, the diameter of the stirring disc is 0.3 times of the true diameter of the reactor, and the center of the stirring disc is provided with a sliding hole 10 the diameter of which is slightly larger than that of the sliding rod.
As shown in figure 2, the middle part of the stirring plate is provided with four circulation holes 11, the diameters of the circulation holes are the same, and the circulation holes are uniformly distributed on the circumference. The sliding rod and the bracket are made of stainless steel materials, the stirring disc is made of iron materials, and fluoroplastic materials wrap the periphery of the stirring disc.
An electromagnet 12 for magnetically attracting the stirring disc is arranged above the reactor, and the electromagnet can attract the stirring disc to move upwards when being electrified. The upper end of the slide bar is provided with a stop switch 9 for stopping the electromagnet, and the lower end of the slide bar is provided with an operation switch 8 for operating the electromagnet. After the electromagnet is electrified, the stirring disc is upwards attracted to operate due to electromagnetic attraction, after the stirring disc operates to the stop switch 9 at the upper end, the electromagnet is powered off, the stirring disc operates downwards under the action of gravity, and after the stirring disc operates to the operation switch 8 at the lower end, the electromagnet is powered on, and the stirring disc operates upwards again and reciprocates.
When the stirring disc moves upwards or downwards, the solution in the reactor is driven to move up and down, so that the solution is fully mixed. The running speed of the stirring disc can be controlled by adjusting the power of the electromagnet.
Four flow holes 11 are arranged in the middle of the stirring plate, so that a small amount of solution can flow through the flow holes, the solution turbulence can be prevented, and the stirring plate can stably run upwards or downwards.
The reaction device is used for cell culture, nutrient solution and a certain amount of cultured cells are added, the electromagnet is electrified to drive the stirring disc to move upwards at the upward running speed of about 30mm/s and drive the liquid to turn over up and down, after the stop switch is operated, the electromagnet is powered off, the stirring disc runs downwards under the action of gravity at the running speed of about 20 mm/s. By culturing in the reaction apparatus, the cells can be ensured to grow well.
Example 2
The embodiment 2 of the invention is basically the same as the embodiment 1, and the difference is that the stirring disc comprises four sliding holes 10 which are uniformly distributed in the circumferential direction, and similarly, the number of the sliding rods 7 on the bracket is four, and the four sliding rods are matched with the sliding holes, so that the stirring disc can run up and down on the sliding rods. The four sliding rods are arranged, so that the stirring disc can run up and down more stably. Secondly, no circulation hole is arranged on the stirring disc. And thirdly, the diameter of the stirring disc is 0.4 times of the diameter of the reactor. Fourthly, the height of the sliding rod is 0.8 times of the height of the reactor.

Claims (6)

1. The utility model provides a reaction unit suitable for cell growth, includes the reactor of being made by the stainless steel, is provided with heating device, discharge gate, feed inlet, detection mouth on the reactor, its characterized in that: a support is fixed at the bottom of the reactor, a vertical sliding rod positioned in the middle of the reactor is fixed on the support, a disc-shaped stirring disc capable of moving up and down on the sliding rod is arranged on the sliding rod, a sliding hole with the diameter slightly larger than that of the sliding rod is arranged in the center of the stirring disc, the sliding rod and the support are made of stainless steel materials, and the stirring disc is made of iron materials; an electromagnet capable of magnetically attracting the stirring disc is arranged above the reactor, a stop switch for stopping the electromagnet is arranged at the upper end of the sliding rod, and an operation switch for operating the electromagnet is arranged at the lower end of the sliding rod.
2. A reaction device adapted for cell growth according to claim 1, wherein: the periphery of the stirring plate is wrapped with a plastic material.
3. A reaction device adapted for cell growth according to claim 1, wherein: the stirring disc is provided with four circulation holes which are uniformly distributed on the circumference, and the diameters of the circulation holes are the same.
4. A reaction device adapted for cell growth according to claim 1, wherein: the height of the sliding rod is 0.5-1 times of the height of the reactor, and the diameter of the stirring disc is 0.2-0.5 times of the diameter of the reactor.
5. A reaction device adapted for cell growth according to claim 1, wherein: the number of the sliding rods is three, and the stirring disc is provided with three sliding holes matched with the sliding rods.
6. A reaction device adapted for cell growth according to claim 1, wherein: the four parallel sliding rods are arranged, and the stirring disc is provided with four sliding holes matched with the sliding rods.
CN202210034494.XA 2022-01-13 2022-01-13 Reaction device suitable for cell growth Active CN114480121B (en)

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CN114480121B CN114480121B (en) 2022-12-09

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4512666A (en) * 1984-02-24 1985-04-23 Corning Glass Works Adjustable height magnetic stirrer
CN207031441U (en) * 2017-05-22 2018-02-23 皖西学院 A kind of constant temperature food fermentation device
CN108473954A (en) * 2015-11-02 2018-08-31 株式会社美加细胞 Use the manufacturing method for the blood platelet for moving back and forth agitating device
CN109439519A (en) * 2018-11-09 2019-03-08 郑州韦尔特生物科技有限公司 Homogenization apparatus is used in a kind of test of bioengineering
CN110914402A (en) * 2018-02-15 2020-03-24 富有干细胞株式会社 Cell culture device

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4512666A (en) * 1984-02-24 1985-04-23 Corning Glass Works Adjustable height magnetic stirrer
CN108473954A (en) * 2015-11-02 2018-08-31 株式会社美加细胞 Use the manufacturing method for the blood platelet for moving back and forth agitating device
CN207031441U (en) * 2017-05-22 2018-02-23 皖西学院 A kind of constant temperature food fermentation device
CN110914402A (en) * 2018-02-15 2020-03-24 富有干细胞株式会社 Cell culture device
CN109439519A (en) * 2018-11-09 2019-03-08 郑州韦尔特生物科技有限公司 Homogenization apparatus is used in a kind of test of bioengineering

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