CN114470121B - Medicine for treating osteoarthritis and preparation method thereof - Google Patents

Abstract

The invention discloses a pharmaceutical composition for treating osteoarthritis, which is prepared from chick embryo, earthworm, fructus psoraleae, scalded drynaria rhizome, borneol, gardenia, herba lycopi, radix angelicae, nux vomica, large-leaf gentian root, herba lycopodii, herba siegesbeckiae, glabrous greenbrier rhizome, herba celandine prescription and auxiliary materials. The external plaster has the effects of promoting qi and blood circulation, dredging collaterals and extending tendons, and relieving swelling and pain, is suitable for people suffering from pain around the joints and knees, adverse movement or limited movement of the joints, and light and moderate swelling of the knee joints caused by knee osteoarthritis by coating plaster on injured bones, joints and soft tissues, and has the advantages of obvious curative effect, safety, no toxic or side effect, convenient effect, stable drug property and good pain relieving effect.

Description

Medicine for treating osteoarthritis and preparation method thereof

The invention field:

the invention relates to a medicine for treating osteoarthritis (also called chick embryo earthworm paste) and a preparation method thereof, belonging to the technical field of medicines.

Technical background:

osteoarthritis is a degenerative disease, which is a degenerative injury of articular cartilage, reactive hyperplasia of articular edges and subchondral bones, which is caused by various factors such as aging, obesity, strain, trauma, congenital anomalies of joints, joint deformity and the like. The disease is usually found in middle-aged and elderly people, and is best caused by heavy-duty joints and joints with high activity (such as cervical vertebra, lumbar vertebra, knee joint, hip joint, etc.). Overload or the use of these joints can promote the occurrence of degenerative changes. Clinical manifestations are slow-developing joint pain, tenderness, stiffness, joint swelling, limited mobility, joint deformity, and the like. The current treatment methods have the following aspects: firstly, anti-inflammatory analgesic drugs can alleviate or control symptoms, but should be used carefully after evaluating patient risk factors and should not be taken for a long period of time. Second, the use of chondroprotective agents such as glucosamine sulfate has symptomatic and functional improvement effects, while long-term administration may delay the structural progression of the disease. Finally, for advanced cases, under conditions where the whole body can withstand the procedure, there is a risk and cost of the procedure for manual joint replacement.

The method is an aquarium recipe, and the aquarium people live in areas with high temperature, humidity, raininess and severe pestilence for a long time, accumulate rich medical experience in the long-term production and living practice of the aquarium people in the geographical environment with inconvenient traffic and economic lag, and gradually form the aquarium medicine with national characteristics, which is suitable for the characteristics of the aquarium people. The traditional Chinese medicine plaster is directly applied to the body surface, and the plaster penetrates into the skin through the spicy and fragrant channel-extending extremely strong channel-guiding medicine, and is internally transmitted to the channels and collaterals and viscera, so that the effects of promoting qi and activating blood, dredging collaterals and stretching tendons, and relieving swelling and pain are achieved.

The patent application is supported by the project of the modern research of traditional Chinese medicines in the key research and development plan of the state of the scientific and technological department of 2019, the diagnosis and treatment technology, the method and the prescription medicine arrangement and demonstration research of fifteen ethnic doctors for preventing and treating common diseases (project number: 2019YFC1712500 and subject number: 2019YFC 1712504).

The invention comprises the following steps:

the invention aims to provide a medicine (also called chick embryo earthworm paste) for treating osteoarthritis and a preparation method thereof. The medicine has effects of activating qi-flowing, promoting blood circulation, dredging collaterals, extending tendons, relieving swelling and pain, and is suitable for osteoarthritis patients with arthralgia, local swelling, and adverse flexion and extension of joint.

In order to solve the technical problems, the invention is realized by adopting the following technical scheme:

the medicine for treating osteoarthritis is prepared with chicken embryo 500-1500 weight portions, earthworm 100-900 weight portions, malaytea scurfpea fruit 100-900 weight portions, drynaria rhizome 100-900 weight portions, borneol 10-190 weight portions, cape jasmine 200-1000 weight portions, herba lycopi 50-800 weight portions, dahurian angelica root 20-500 weight portions, nux vomica 10-190 weight portions, gentiana macrophylla 100-900 weight portions, lycopodium clavatum 100-900 weight portions, herba siegesbeckiae 100-900 weight portions, glabrous greenbrier rhizome 100-900 weight portions, celandine 80-650 weight portions and supplementary material.

Specifically, the medicine for treating osteoarthritis is prepared from 900-1100 parts by weight of chick embryo, 400-600 parts by weight of earthworm, 400-600 parts by weight of fructus psoraleae, 400-600 parts by weight of hot drynaria rhizome, 50-150 parts by weight of borneol, 500-700 parts by weight of cape jasmine, 300-500 parts by weight of herba lycopi, 100-300 parts by weight of angelica dahurica, 50-150 parts by weight of nux vomica, 400-600 parts by weight of gentiana macrophylla, 400-600 parts by weight of lycopodium clavatum, 400-600 parts by weight of herba siegesbeckiae, 400-600 parts by weight of glabrous greenbrier rhizome and 200-400 parts by weight of celandine.

More specifically, the medicine for treating osteoarthritis is prepared from 1000 parts of chick embryo, 500 parts of earthworm, 500 parts of fructus psoraleae, 500 parts of hot drynaria rhizome, 100 parts of borneol, 600 parts of gardenia, 400 parts of herba lycopi, 200 parts of radix angelicae, 100 parts of nux vomica, 500 parts of large-leaf gentian root, 500 parts of lycopodium clavatum, 500 parts of herba siegesbeckiae, 500 parts of glabrous greenbrier rhizome, 300 parts of herba celandine and auxiliary materials according to weight parts.

The preparation method of the medicine for treating osteoarthritis comprises the following steps: all the medicinal materials are weighed according to the proportion, processed according to the conventional preparation method, combined with pharmaceutically acceptable auxiliary materials and matrixes in the medicine, and prepared into corresponding pharmaceutical preparations.

Specifically, the preparation method of the medicine for treating osteoarthritis comprises the following steps: weighing the medicinal materials according to the proportion of the formula, pulverizing the medicinal materials except borneol into coarse powder, processing according to a conventional preparation method, refining into paste, adding borneol fine powder, uniformly stirring and coating on a substrate to prepare a corresponding medicinal preparation.

The medicinal preparation is plaster, rubber plaster or gel plaster.

The plaster is prepared by the following steps: weighing the medicinal materials (deicing slices) according to the prescription proportion, and crushing into coarse powder; adding coarse powder (deicing flake) of medicinal materials after slightly heating 2 times of oleum Sesami, heating and stirring continuously until the medicinal materials are fried to dark brown on the surface and brown inside; the temperature reaches 220 ℃, the dregs are fished out after the frying, and the oil after the dregs are removed is medicinal oil; continuously cooking the medicinal oil until the temperature of the oil rises to 320 ℃, and cooking with medium fire until a small amount of medicinal oil is dropped into water and is not scattered into beads; after the medicinal oil is refined, the red lead with the amount of 50% is prepared from the medicinal oil by removing fire, stirring is carried out while adding, the medicinal oil is stirred into a viscous paste, the plaster is not sticky, and the plaster is obtained by continuous wire drawing; soaking in cold water to remove toxic substances, changing water every day, and continuously for seven days; placing the plaster mass in a container, melting on slow fire, adding Borneolum Syntheticum fine powder according to the prescription proportion, and uniformly coating on plaster cloth.

The rubber paste is prepared by the following steps: weighing all the medicinal materials (deicing slices) according to the prescription proportion, soaking the medicinal materials in 6 times of 50% ethanol for 0.5h, carrying out reflux extraction for 2 times at 80 ℃ for 2 hours each time, mixing the two extracting solutions, uniformly mixing, concentrating the mixed solution into 80% of the original medicinal liquid, filtering the hot solution, and concentrating the filtrate into an extract with relative density of 1.20-1.30 at 80 ℃; taking 10 times of raw rubber, drying at 50 ℃, cutting into strips, plasticating into net-shaped sheets in a rubber mixing machine, spreading for open cooling, eliminating static electricity, soaking in proper amount of gasoline for 20 hours, moving into a paste making machine after completely swelling into gel, stirring for 3 hours, adding 2% of vaseline, 5% of lanolin, 10% of zinc oxide and 1% of rosin into the paste, preparing a plug by adding the human extract and borneol which is prepared into fine powder according to the prescription proportion, stirring uniformly, pasting, quick drying, lining, cutting and packaging to obtain the finished product.

The gel paste is prepared by the following steps: weighing all the medicinal materials (deicing slices) according to the prescription proportion, soaking the medicinal materials (deicing slices) in 8 times of 50% ethanol for 0.5h, carrying out reflux extraction for 3 times at 80 ℃ for 1 hour each time, merging the three extracting solutions, uniformly mixing, concentrating the mixed solution into 80% of the original medicinal liquid, filtering while the mixed solution is hot, concentrating the filtrate into an extract with the relative density of 1.20-1.30 at 80 ℃, and adding water with the preparation amount of 50% into the extract for later use; mixing sodium polyacrylate 20g, gelatin 10g and silica gel micropowder 2g, adding glycerol 30g, pouring the mixture into the extract, adding Borneolum Syntheticum fine powder according to the prescription proportion, uniformly coating on non-woven fabric, oven drying for 0.5 hr, solidifying at room temperature for 48 hr, covering with backing layer, and sealing and packaging.

When the pharmaceutical composition is prepared into a medicament, the medicament with unit dosage can contain 0.1-1000mg of the pharmaceutical active substance of the invention, and the balance is pharmaceutically acceptable auxiliary materials. Pharmaceutically acceptable excipients can be 0.1-99.9% by weight of the total weight of the formulation.

The medicine is prepared from chick embryo, earthworm, malaytea scurfpea fruit, dried drynaria rhizome, borneol, cape jasmine fruit, herba lycopi, dahurian angelica root, nux vomica, large-leaf gentian root, lycopodium clavatum, herba siegesbeckiae, glabrous greenbrier rhizome, celandine and auxiliary materials. The properties and efficacy of each component are as follows: the chick embryo is chick embryo (English name: chick embryo or embryonated egg), which is an egg which is hatched but not hatched, the embryo in the egg is developed, the eggshell is peeled off, the chick embryo can be seen, the chick embryo is fine and soft, and even the chick bone can be seen; the naturally hatched live chick embryo is selected, and tens of millions of dead chick embryos are not edible; has the effects of invigorating qi, caring skin, promoting health and replenishing blood, and is described in Ben Cao gang mu: "chicken embryo egg has the functions of treating osteoarthritis, metaosteoarthritis, head crazy disease and limb crazy and cataract; the chicken embryo has good nutritive value, contains rich mineral substances and vitamins, has higher nutritive value than common eggs, can improve the selenium deficiency condition, and has anti-aging effect. The Dilong product is dried by Eimeria tenella Pheretima aspergillum (E.Perrie), eimeria necatrix Pheretima vulgaris Chen, eimeria necatrix Pheretima guillelmi (Michaelsen) or Eimeria armigera Pheretima pectinifera Michaelsen; the former is known as Guangdong earthworm and the latter three are known as Hu Lumbricus; capturing Lumbricus in spring to autumn, capturing Lumbricus in summer, cutting open abdomen in time, removing viscera and silt, cleaning, sun drying or drying at low temperature; salty taste and cold nature; enter liver, spleen and bladder meridians; has effects in clearing away heat, arresting convulsion, dredging collaterals, relieving asthma, and promoting urination; can be used for treating hyperpyrexia, unconsciousness, convulsion, joint paralysis, numbness of limbs, hemiplegia, lung heat, asthma, cough, edema, and oliguria. Fructus Psoraleae is dried mature fruit of Psoralea corylifolia Psoralea corylifolia L of Leguminosae, harvesting fruit sequence in autumn, sun drying, rubbing out fruit, and removing impurities; pungent and bitter in taste and warm in nature; enter kidney and spleen meridians; has effects in warming kidney, supporting yang, invigorating qi, relieving asthma, warming spleen, and relieving diarrhea; the topical preparation has effects of dispelling pathogenic wind and removing speckle; can be used for treating kidney yang deficiency, sexual impotence, spermatorrhea, enuresis, frequent urination, cold pain of waist, asthma due to kidney deficiency, and diarrhea due to five elements; it can be used for treating vitiligo and alopecia areata. The preparation method of dried rhizome of drynaria rhizome of drynariaceae Davallia mariesii Moore ex Bak comprises heating sand in a pot, adding clean drynaria rhizome or tablet, stirring, scalding, removing sand, removing hair, and cooling; bitter taste and warm nature; enter kidney and liver meridians; has effects of invigorating kidney, strengthening bone, relieving pain, dispelling blood stasis, relieving pain, promoting reunion of bone, and treating toothache, lumbago, chronic diarrhea, etc.; can be used for treating lumbago due to kidney deficiency, tinnitus, deafness, odontoseisis, traumatic injury, contusion, and tendons and bones injury; it is used for treating alopecia areata and vitiligo. Borneol is prepared from fresh branches and leaves of Cinnamomum camphora plant rod Cinnamomum camphora (L.) Presl by extracting and processing; pungent and bitter in taste and cool in nature; the heart, spleen and lung channels have the functions of inducing resuscitation and refreshing, and removing heat and relieving pain; can be used for treating fever, unconsciousness, convulsion, apoplexy, phlegm syncope, qi stagnation syncope, middle-jiao coma, chest pain, conjunctival congestion, aphtha, laryngopharynx swelling and pain, and ear canal purulence. Fructus Gardeniae is dried mature fruit of fructus Gardeniae Gardenia jasminoides Ellis of Rubiaceae plant; harvesting when the fruits are ripe and red-yellow for 9-11 months, removing the fruit stalks and impurities, steaming to the upper air or putting the fruits into boiling water for scalding slightly, taking out and drying; bitter in flavor and cold in nature. Return to heart, lung and triple energizer meridians; has effects in purging pathogenic fire, relieving restlessness, clearing away heat and dampness, cooling blood, and removing toxic substances; the external use has the effects of detumescence and pain relief; can be used for treating febrile vexation, jaundice due to damp-heat, stranguria, hematemesis, conjunctival congestion, swelling and pain, and pyocutaneous disease due to pathogenic fire; it is indicated for sprain, contusion and pain. Herba Lycopi is dry aerial part of Labiatae plant Pachyrhizus seedling Lycopus lucidus turcz. Var. Hirtus Regel; harvesting in summer and autumn when Ji Lianshe flourish, and sun drying; bitter and pungent taste and slightly warm nature; enter liver and spleen meridians; has effects of promoting blood circulation, regulating menstruation, removing blood stasis, resolving carbuncle, inducing diuresis and relieving edema; it can be used for treating menoxenia, amenorrhea, dysmenorrhea, puerperal abdominal pain due to blood stasis, sore, carbuncle, toxic swelling, edema, and ascites. Radix Angelicae Dahuricae is dry root of radix Angelicae Dahuricae Angelica dahurica (Fisch. Ex Hoffm.) or radix Angelicae Dahuricae Angelica dahurica (Fisch. Ex Hoffm.) of Umbelliferae; collecting in summer and autumn She Huangshi, removing fibrous root and silt, and sun drying or low temperature drying; pungent taste and warm nature; the stomach, large intestine and lung channels; has effects of relieving exterior syndrome, dispelling cold, dispelling pathogenic wind, relieving pain, dredging nasal orifices, eliminating dampness, stopping leukorrhagia, and relieving swelling and pus; can be used for treating common cold, osteoarthritis, osteodynia, nasal obstruction, nasal discharge, allergic rhinitis, nasosinusitis, toothache, leukorrhagia, and pyocutaneous disease. Semen Strychni is dry mature seed of Strychnos nux vomica L of Strychnos of Strychnaceae; harvesting mature fruits in winter, taking out seeds, and sun-drying; bitter taste, warm nature and strong toxicity; enter liver and spleen meridians; has effects of dredging collaterals, relieving pain, resolving hard mass and detumescence; can be used for treating traumatic injury, fracture swelling and pain, rheumatism, numbness, paralysis, carbuncle, skin sore, and sore throat. The radix Gentianae Marcrophyllae is dry root of Gentiana Marcrophylla Gentiana macrophylla pall. Of Gentianaceae, gentiana Marcrophylla Gentiana straminea maxim. Of Gentianaceae, gentiana Marcrophylla Gentiana crassicaulis Duthie ex Burk. Or Gentiana microphylla Gentiana dahurica Fisch. Of Gentiana Marcrophylla; the former three are respectively known as "Gentiana Marcrophylla" and "Gentiana Marcrophylla Crohn et al" according to different properties, and the latter is known as "Small Gentiana Marcrophylla"; digging in spring and autumn to remove sediment; sun-drying radix Gentianae Marcrophyllae and radix Gentianae Marcrophyllae, and piling to "sweat" until the surface is reddish yellow or yellowish gray, or directly sun-drying without "sweat"; rubbing off black skin of radix Gentianae Marcrophyllae when it is fresh, and sun drying; pungent and bitter in taste and mild in nature; enter stomach, liver and gallbladder meridians; has effects of dispelling pathogenic wind and dampness, clearing away damp-heat, relieving arthralgia, and removing deficiency heat; can be used for treating rheumatalgia, apoplexy, hemiplegia, spasm of tendons and vessels, joint pain, jaundice due to damp-heat, hectic fever due to bone steaming, infantile malnutrition, and fever. Herba Lycopodii is dried whole plant of Pinus koraiensis Lycopodium japonicum thunder of Pinaceae; harvesting in summer and autumn when the stem and leaf are luxuriant, removing impurities, and sun drying; slightly bitter and pungent in taste and warm in nature; enter liver, spleen and kidney meridians; has effects of dispelling pathogenic wind, removing dampness, relieving rigidity of muscles and activating collaterals; can be used for treating joint pain and difficulty in flexing and extending. Herba Siegesbeckiae is dry aerial part of Siegesbeckia orientalis L. Of Compositae, siegesbeckia Siegesbeckia, siegesbeckia pubescens Makino of glandula Siegesbeckia or Siegesbeckia glabrescens Makino of Siegesbeckia pubescens; the flowers can be cut before and during summer and autumn, impurities are removed and the flowers are dried in the sun; pungent and bitter in flavor and cold in nature; enter liver and kidney meridians; has effects in dispelling pathogenic wind and dampness, benefiting joint, removing toxic substances, and promoting blood circulation; can be used for treating rheumatalgia, tendons and bones weakness, soreness of waist and knees, paralysis of limbs, hemiplegia, rubella, and skin sore. Rhizoma Smilacis Glabrae is dried rhizome of Smilax glabra Roxb. Digging in summer and autumn, removing fibrous root, cleaning, and drying; or cutting into slices while fresh, and drying; sweet and light in taste and neutral in nature; enter liver and stomach meridians; has effects of removing toxic substances, removing dampness, and dredging joint; can be used for treating limb contracture, bone and muscle pain caused by syphilis and mercury poisoning; damp-heat stranguria with turbid urine, leukorrhagia, carbuncle, scrofula and scabies. Herba Chelidonii is dried whole herb of herba Chelidonii Chelidonium majus L of Papaveraceae; collected in summer and autumn, removed with sediment, and dried in shade or sun-dried; bitter in taste and cool in nature; is poisonous; spleen and lung meridian entered; has effects of relieving spasm and pain, relieving cough and asthma; can be used for treating gastric abscess, cough, asthma, and pertussis.

And (3) square solution: the theory of external treatment, i.e. the theory of internal treatment, and the herbs of external treatment, i.e. the herbs of internal treatment, are described in the text of reason ; the other is the normal one. External treatment means that the medicine can directly reach the affected part. The plaster is used to block the qi and make the medicine enter the striae and striae from pores, through the channels or be extracted, so that the medicine is more beneficial than the medicine taking method. Modern pharmacological research shows that the externally applied meat traditional Chinese medicine for promoting blood circulation, removing blood stasis and promoting tissue regeneration contains a large amount of immune activators, plays a role in regulating among cells and between cells and body fluid, quickens bleeding and edema absorption, and causes tissue hypoxia, lactic acid accumulation and pH reduction, thereby strengthening aerobic metabolism and promoting regeneration after muscle fiber injury, and is a basis for promoting healing of injured tissues.

The chick embryo earthworm paste mainly comprises 14 traditional Chinese medicines of chick embryo, earthworm, fructus psoraleae, hot drynaria rhizome, ice, herba siegesbeckiae, glabrous greenbrier rhizome, celandine and the like. The prescription takes the functions of promoting blood circulation to remove blood stasis, dispelling wind and removing dampness, reducing swelling and relieving pain as a treatment rule, takes the hot drynaria rhizome as a warm medicine, has pungent taste, activates collaterals, tonifies kidney, and the fructus psoraleae warms water and soil, digests diet, reaches spleen and stomach, converges and smoothes and leaks, and supplements spleen, and both spleen and kidney are used as monarch medicines. The Chinese medicinal composition is added with cape jasmine, herba lycopi, dahurian angelica root, nux vomica, large-leaf gentian root, lycopodium clavatum for detumescence and resolving hard mass, dispelling blood stasis and eliminating symptoms, dispelling wind and removing dampness, relaxing tendons and activating collaterals, and dispersing blood stasis and relieving pain. Siegesbeckia herba is used for treating quadriplegia, arthralgia and myalgia, weakness of waist and knees, malaria, furuncle, swelling and toxicity, and traumatic hemorrhage. The medicines are used as ministerial medicines and assist the strength of one arm of monarch medicine. Rhizoma smilacis glabrae is added to strengthen tendons and bones, remove rheumatism and promote articulation; herba Chelidonii has effects of relieving pain, promoting urination and removing toxic substances; borneol can be used for dredging orifices, dispersing joint stagnation fire, and relieving swelling and pain. The three medicines are used together, so that only the medicines for preventing the monarch and minister from moving qi and overabundant blood and causing heat toxin due to long-term blood stasis can promote the overflow of blood outside the pulse to remove water and induce diuresis, and the adjuvant medicine has the functions of clearing heat and removing blood stasis without damaging vital qi, and penetrating and promoting diuresis and removing blood stasis to dredge channels and collaterals. Lumbricus is good at blood and meat, and better than herbal medicine in penetrating property, so it can dredge and activate meridians and collaterals, and is used as an adjuvant. The good effect of using the blood and meat of the chickens coming out of the shell as a guiding drug for replenishing qi, replenishing essence and promoting tissue regeneration. The medicine can directly act on the disease place through the skin, and the functions of the medicines are exerted. The traditional Chinese medicine composition is composed of the whole formula, monarch drugs, minister drugs, assistant drugs and guide drugs, and complement each other, and the combination of the drugs can dredge the striae and skin, smooth qi and blood, promote blood circulation, reduce swelling, nourish blood, cool blood, dissipate blood stasis, relieve pain, and avoid forgetting to strengthen bones (and cartilage) to generate new life. Clinical observation shows that the chick embryo earthworm paste is obviously better than the pain and swelling of patients before treatment after the application of the chick embryo earthworm paste is applied to the acute onset period of osteoarthritis for 4-5 days, has obvious effects of easing pain, diminishing inflammation and diminishing swelling, and can be achieved by improving microcirculation, affecting active media secreted by tissue cells, promoting metabolism of local inflammatory factors, preventing aggregation, vasoconstriction and thrombosis of blood platelets, reducing tissue injury and enhancing the repairing capability of tissues. The medicine is an ideal medicine for treating osteoarthritis in the acute stage, soft tissue injury, tendons and bones around joints and pain.

The external preparation for treating osteoarthritis is suitable for pain of joints and periknee caused by knee osteoarthritis, unfavorable movement and limited movement of joints, and light and moderate swelling of knee joints.

The invention selects the external ointment to prepare the pharmaceutical preparation from the aspects of safety, stability and curative effect. The plaster is not too much or too short in the preparation process of the plaster, if the plaster is too short, the efficacy is difficult to be obtained, and if the plaster is too much, the viscosity of the plaster is affected. The lower pill should be used to control the fire and dosage, and the low temperature affects the combination of the pill and the oil, which is not glossy. The ointment is tender when the amount of the red lead is small, is easy to run off and has no fixing effect. When the pill amount is large, the paste is old, crisp and not sticky, and the paste is easy to fall off. The extraction rate of the effective components is affected by the ethanol extraction of the medicinal materials added into the rubber paste and the gel paste, and if the ethanol is insufficient, the solvent cannot be soaked in the medicinal materials, and if the heating and extraction time is insufficient, the medicinal materials cannot be fully extracted, so that the leaching rate of the medicinal materials is affected. Too long heating extraction time and too high temperature can lead to decomposition of the active ingredients. Instead, the extraction rate of the medicinal materials is reduced. The inventor proves that by experimental study: the effective components can be basically dissolved out after 2-3 times of heating and cooking.

Compared with the prior art, the invention selects the chick embryo, earthworm, fructus psoraleae, rhizoma drynariae, borneol, gardenia, herba lycopi, radix angelicae, nux vomica, gentiana macrophylla, lycopodium clavatum, herba siegesbeckiae, glabrous greenbrier rhizome and celandine prescription, is an aquarium prescription, is supported by national science and technology department projects, has the effects of promoting qi and activating blood, dredging collaterals and stretching tendons, and relieving swelling and pain, is suitable for the people suffering from osteoarthritis with arthralgia, local swelling and joint bending and stretching disadvantages by coating plaster on injured bones, joints and soft tissues, has the advantages of obvious curative effect, safety, no toxic or side effect, convenient effect, stable medicine property and good pain relieving effect, and achieves the aim of the invention.

The invention is further illustrated by the following examples, which are not intended to be limiting.

The specific embodiment is as follows:

example 1:

prescription: 1000g of chick embryo, 500g of earthworm, 500g of malaytea scurfpea fruit, 500g of hot drynaria rhizome, 100g of borneol, 600g of gardenia, 400g of herba lycopi, 200g of angelica dahurica, 100g of nux vomica, 500g of large-leaf gentian root, 500g of lycopodium clavatum, 500g of herba siegesbeckiae, 500g of glabrous greenbrier rhizome and 300g of celandine.

The process comprises the following steps: weighing the medicinal materials (deicing slices) according to the prescription proportion, and crushing into coarse powder; adding coarse powder (deicing flake) of medicinal materials after slightly heating 2 times of oleum Sesami, heating and stirring continuously until the medicinal materials are fried to dark brown on the surface and brown inside; the temperature reaches 220 ℃, the dregs are fished out after the frying, and the oil after the dregs are removed is medicinal oil; continuously cooking the medicinal oil until the temperature of the oil rises to 320 ℃, and cooking with medium fire until a small amount of medicinal oil is dropped into water and is not scattered into beads; after the medicinal oil is refined, the red lead with the amount of 50% is prepared from the medicinal oil by removing fire, stirring is carried out while adding, the medicinal oil is stirred into a viscous paste, the plaster is not sticky, and the plaster is obtained by continuous wire drawing; soaking in cold water to remove toxic substances, changing water every day, and continuously for seven days; placing the plaster mass in a container, melting with slow fire, adding Borneolum Syntheticum fine powder according to the prescription proportion, and uniformly coating on plaster cloth to obtain plaster.

The usage amount is as follows: the plaster is applied to the affected part after being heated by hot water kettle (cup) or alcohol lamp or candle, and the plaster is applied to the affected part for 1-2 weeks.

Example 2:

prescription: 500g of chick embryo, 100g of earthworm, 100g of malaytea scurfpea fruit, 100g of hot drynaria rhizome, 10g of borneol, 200g of gardenia, 50g of herba lycopi, 20g of angelica dahurica, 10g of nux vomica, 100g of large-leaf gentian root, 100g of lycopodium clavatum, 100g of herba siegesbeckiae, 100g of glabrous greenbrier rhizome and 80g of celandine.

The process comprises the following steps: weighing all the medicinal materials (deicing slices) according to the prescription proportion, soaking the medicinal materials in 6 times of 50% ethanol for 0.5h, carrying out reflux extraction for 2 times at 80 ℃ for 2 hours each time, mixing the two extracting solutions, uniformly mixing, concentrating the mixed solution into 80% of the original medicinal liquid, filtering the hot solution, and concentrating the filtrate into an extract with relative density of 1.20-1.30 at 80 ℃; taking 10 times of raw rubber, drying at 50 ℃, cutting into strips, plasticating into net-shaped sheets in a rubber mixing machine, spreading for open cooling, eliminating static electricity, soaking in proper amount of gasoline for 20 hours, moving into a paste making machine after completely swelling into gel, stirring for 3 hours, adding 2% of vaseline, 5% of lanolin, 10% of zinc oxide and 1% of rosin into the paste, preparing a plug by time, adding human extract and borneol which is prepared into fine powder according to a prescription proportion, stirring uniformly, coating the paste, quick drying, lining, cutting and packaging to obtain the rubber paste.

The usage amount is as follows: the plaster is externally applied, directly applied to the affected part, and can be properly hot-applied for 5 minutes after being applied, and can be continuously applied for 1-2 weeks.

Example 3:

prescription: 650g of chick embryo, 100g of earthworm, 800g of malaytea scurfpea fruit, 550g of hot drynaria rhizome, 50g of borneol, 300g of cape jasmine, 800g of herba lycopi, 20g of angelica dahurica, 190g of nux vomica, 900g of large-leaf gentian root, 900g of lycopodium clavatum, 900g of herba siegesbeckiae, 900g of glabrous greenbrier rhizome and 650g of celandine.

The process comprises the following steps: weighing all the medicinal materials (deicing slices) according to the prescription proportion, soaking the medicinal materials (deicing slices) in 8 times of 50% ethanol for 0.5h, carrying out reflux extraction for 3 times at 80 ℃ for 1 hour each time, merging the three extracting solutions, uniformly mixing, concentrating the mixed solution into 80% of the original medicinal liquid, filtering while the mixed solution is hot, concentrating the filtrate into an extract with the relative density of 1.20-1.30 at 80 ℃, and adding water with the preparation amount of 50% into the extract for later use; mixing sodium polyacrylate 20g, gelatin 10g and silica gel micropowder 2g, adding glycerol 30g, pouring the mixture into the extract, uniformly coating Borneolum Syntheticum fine powder according to the prescription proportion on non-woven fabric, oven drying for 0.5 hr, solidifying at room temperature for 48 hr, covering with backing layer, and sealing and packaging to obtain gel paste.

The usage amount is as follows: for external application, the backing layer is torn off and then directly applied to the affected part for 1-2 weeks.

Example 4:

prescription: 1500g of chick embryo, 900g of earthworm, 900g of malaytea scurfpea fruit, 900g of hot drynaria rhizome, 190g of borneol, 1000g of cape jasmine, 800g of herba lycopi, 500g of angelica dahurica, 120g of nux vomica, 700g of large-leaf gentian root, 500g of lycopodium clavatum, 500g of herba siegesbeckiae, 300g of glabrous greenbrier rhizome and 250g of celandine.

The process comprises the following steps: weighing the medicinal materials (deicing slices) according to the prescription proportion, and crushing into coarse powder; adding coarse powder (deicing flake) of medicinal materials after slightly heating 2 times of oleum Sesami, heating and stirring continuously until the medicinal materials are fried to dark brown on the surface and brown inside; the temperature reaches 180 ℃, the dregs are fished out after the frying, and the oil after the dregs are removed is medicinal oil; continuously cooking the medicinal oil until the temperature of the oil rises to 360 ℃, and cooking with medium fire until a small amount of medicinal oil is dropped into water and is not scattered into beads; after the medicinal oil is refined, 60% of red lead is prepared from the medicinal oil by heating, stirring is carried out while adding, the medicinal oil is stirred into a viscous paste, the paste is not sticky, and the paste is obtained after continuous wire drawing; soaking in cold water to remove toxic substances, changing water every day, and continuously for seven days; placing the plaster mass in a container, melting with slow fire, adding Borneolum Syntheticum fine powder according to the prescription proportion, and uniformly coating on plaster cloth to obtain plaster.

The usage amount is as follows: the plaster is applied to the affected part after being heated by hot water kettle (cup) or alcohol lamp or candle, and the plaster is applied to the affected part for 1-2 weeks.

Example 5:

prescription: 1000g of chick embryo, 600g of earthworm, 100g of malaytea scurfpea fruit, 700g of hot drynaria rhizome, 190g of borneol, 500g of cape jasmine, 200g of herba lycopi, 155g of dahurian angelica root, 100g of nux vomica, 250g of large-leaf gentian root, 600g of common clubmoss herb, 900g of herba siegesbeckiae, 550g of glabrous greenbrier rhizome and 400g of celandine.

The process comprises the following steps: weighing all the medicinal materials (deicing slices) according to the prescription proportion, soaking the medicinal materials in 4 times of 50% ethanol for 0.5h, carrying out reflux extraction for 2 times at 80 ℃ for 1 hour each time, mixing the two extracting solutions, uniformly mixing, concentrating the mixed solution into 80% of the original medicinal liquid, filtering the hot solution, and concentrating the filtrate into an extract with relative density of 1.20-1.30 at 80 ℃; taking 10 times of raw rubber, drying at 50 ℃, cutting into strips, plasticating into net-shaped sheets in a rubber mixing machine, spreading for open cooling, eliminating static electricity, soaking in proper amount of gasoline for 20 hours, moving into a paste making machine after completely swelling into gel, stirring for 3 hours, adding 2% of vaseline, 5% of lanolin, 10% of zinc oxide and 1% of rosin into the paste, preparing a plug by time, adding human extract and borneol which is prepared into fine powder according to a prescription proportion, stirring uniformly, coating the paste, quick drying, lining, cutting and packaging to obtain the rubber paste.

The usage amount is as follows: the plaster is externally applied, directly applied to the affected part, and can be properly hot-applied for 5 minutes after being applied, and can be continuously applied for 1-2 weeks.

Example 6:

prescription: 550g of chick embryo, 800g of earthworm, 150g of malaytea scurfpea fruit, 900g of hot drynaria rhizome, 80g of borneol, 250g of gardenia, 750g of herba lycopi, 300g of angelica dahurica, 190g of nux vomica, 700g of large-leaf gentian root, 800g of lycopodium clavatum, 900g of herba siegesbeckiae, 300g of glabrous greenbrier rhizome and 500g of celandine.

The process comprises the following steps: weighing all the medicinal materials (deicing slices) according to the prescription proportion, soaking the medicinal materials (deicing slices) in 10 times of 50% ethanol for 0.5h, carrying out reflux extraction for 3 times at 80 ℃ for 1.5 h each time, mixing the three extractive solutions, uniformly mixing, concentrating the mixed solution into 80% of the original medicinal liquid, filtering while the mixed solution is hot, concentrating the filtrate into an extract with relative density of 1.20-1.30 at 80 ℃, and adding water with the preparation amount of 50% into the extract for later use; mixing sodium polyacrylate 20g, gelatin 10g and silica gel micropowder 2g, adding glycerol 30g, pouring the mixture into the extract, preparing into fine powder of Borneolum Syntheticum according to the prescription proportion, uniformly coating on non-woven fabric, oven drying for 0.5 hr, solidifying at room temperature for 48 hr, covering with backing layer, and sealing and packaging to obtain gel paste.

The usage amount is as follows: for external application, the backing layer is torn off and then directly applied to the affected part for 1-2 weeks.

The invention carries out a great deal of experimental study, and the following results are obtained by the experimental study:

experimental example: pharmacodynamic study

1. Anti-inflammatory effect assay:

1. experimental animals and materials

Animals: SD rats, SPF grade, 6-8 weeks old, weight 180-220 g, offered by the university of medical science laboratory animal center, guizhou: SCXX (Qian) 2018-0001; experimental animal use license: SYXK (Qian) 2014-001. The animal is routinely raised according to the international experimental animal health care and use guiding principle, the ambient temperature is 23-27 ℃, and the humidity is 50-60%. Reagent: sodium pentobarbital, papain (Sigma, P3250-5 g), chloral hydrate, qingpeng ointment (Qinghai province Tongtian river Tibetan medicine Co., ltd.); ELISA kit (Nanjing build); multifunctional enzyme-labeled instrument (BioTek, usa).

2 Experimental methods

2.1 animals grouping: the SD rats were randomly divided into 6 groups, namely a blank control group, a knee osteoarthritis model group, a positive control group, a low dose group of the patent inventive drug example 1, a high dose group of the patent inventive drug example 1, 6 groups in total, and 10 groups after the SD rats were fed in an SPF environment with a weight of 210-230g and were fed adaptively for 7d without discomfort.

2.2 construction of a rat knee osteoarthritis model: for model and drug group rats, papain was injected into the joint cavities of rats. Under the aseptic condition, rats are anesthetized by a proper amount of chloral hydrate, 0.2mL of papain solution with the concentration of 4% (w/v) is injected into the knee joint cavities of the rats, and the injection is carried out once every 2d, and the total injection is carried out for 2 weeks, so that the knee joint is swollen, and the functional activity disorder is successful in molding. The blank group was injected with physiological saline into the joint cavity.

2.3 drug treatment: after the model is successfully prepared, corresponding drug treatment is carried out, the dosage is converted according to the human mice, and each rat is applied to the affected part according to the weight for 2 times/day. The rats in the blank control group are applied with distilled water to the affected parts; the knee osteoarthritis model group is applied with distilled water to the affected part; the dosage of the positive control group medicine is 0.25mL; the plaster of the patent is applied to the affected part of a rat model of knee osteoarthritis by using the plaster of the invention with the doses of 0.125mL, 0.25mL and 0.5mL according to the low dose, medium dose and high dose of the plaster of the invention of the example 1. The 6 groups of rats were treated 4 weeks after successful dosing with the built model, respectively.

2.4 observation and index detection: during this period, the rats were observed for general conditions (hair gloss, diet, body weight, activity, gait, mental status, etc.). Relevant indicators were tested 4 weeks after drug treatment: after the animals are anesthetized, blood is taken from the abdominal veins, and the animals are kept for later use at-80 ℃ after centrifugation. Detecting serum cytokines Interleukin (IL) -4, IL-10, IL-1 beta, tumor necrosis factor-alpha (TNF-alpha) levels, superoxide dismutase (SOD) and Malondialdehyde (MDA) levels; all rats were sacrificed and disinfected around their bilateral knee joints, 1mL of water for injection was injected through the side needles on both sides of the knee joint, repeatedly aspirated through the suprapatellar ligament insertion into the joint cavity, and then the joint effusion was removed, centrifuged and stored at-80 ℃ for later use. Respectively taking the supernatants of each group to detect the content changes of the main inflammatory factors IL-1 beta, TNF-alpha and matrix metalloproteinase-3 (MMP-3); the ELISA method is used for detecting each factor, and the operation steps are carried out according to the instruction of the kit.

3 results of experiments

3.1 general cases after rat molding: rats showed mental abnormality, slow reaction, swelling of both lower limbs and impaired movement after papain injection for 1 d.

3.2 results Using mean ± Standard deviationRepresenting statistical analysis of data using SPSS20.0 software. The serum and joint cavity volume associated factor results are shown in tables 1 and 2. From the results, the control group, example 1, showed significant differences in the low, medium and high dose groups compared to the model group (P<0.05 The positive control group and the example group are shown to have better anti-inflammatory effect, and the anti-inflammatory effect of the example 1 and the high-dose group is obviously higher than that of the positive control group, which indicates that the medicine disclosed by the invention has better anti-inflammatory effect.

Table 1 comparison of the levels of correlation factors in serum of each experimental group (n=10)

Table 2 comparison of the levels of correlation factors in joint effusions of the experimental groups (n=10)

Conclusion 4

The medicine disclosed by the invention is used for intervening in an SD rat animal model of knee osteoarthritis, and the anti-inflammatory effect of the medicine is tested. The results show that the medicine composition can relieve symptoms such as knee osteoarthritis, rat joint swelling, skin temperature rise and the like, improve the functions of the rat knee joint, and increase the concentration of IL-10 and SOD by reducing the concentration of IL-1 beta, IL-18, TNF-alpha, MDA and MMP-3, relieve inflammation and delay the progress of knee osteoarthritis, and the medicine of the embodiment has the effect of resisting model animal osteoarthritis, and is suitable for preventing and treating osteoarthritis.

2. Analgesic and anti-inflammatory test

Acetic acid torsion test

1 experimental animals and materials: SPF-grade Kunming mice weighing 20+ -2 g were supplied by the university of medical science laboratory animal center, laboratory animal use license: SYXK (Qian) 2014-001. Qingpeng ointment (Qinghai province Tongtian river Tibetan medicine pharmaceutical Co., ltd.)

2, experimental method: after 1 week of conventional adaptive feeding, the mice were randomly divided into 5 groups of 10 mice each, which were the blank control group, the positive control group, the example 1 low dose group, the example 1 medium dose group, and the example 1 high dose group, respectively. The hair removal paste was applied to both sides of the back spine (1 cm. Times.1 cm) of the mice to remove hair, and after 5 minutes, the mice were cleaned with gauze. The mice in the blank group were not treated; the low, medium and high dose example 1 chick embryo earthworm cream groups are respectively smeared at 0.125 ml/dose, 0.25 ml/dose and 0.5 ml/dose on the skin of the shaved part of the mice; the positive control group was smeared with Qingpeng ointment 0.25 mL/piece and fixed with gauze. All mice were applied 1 time a day for 10 days. The mice in group 10d were intraperitoneally injected with 0.02mL/g of 0.6% acetic acid, and immediately the number of twists occurring in the mice within 30min was recorded, and the percentage of analgesia was calculated: percent of analgesia (%) = (number of vehicle-administration group vehicle)/average vehicle-number of vehicle x 100%.

3 experimental results: by mean ± standard deviationThe results are shown in Table 3.

Table 3 comparison of the number of writhing and analgesic rates of mice in each group to glacial acetic acid (n=10)

Conclusion 4 the Chinese medicinal composition provided by the invention comprises chick embryo, earthworm, fructus psoraleae, scalded rhizoma drynariae and other medicaments, and the obtained paste has good medicinal effect, and the experiment proves that the paste has obvious effect on easing pain.

Experiment of mice ear swelling caused by (di) xylene

Experimental animals and groups were the same as in (one) operation. The mice in the 5 groups were each dosed 1 time a day for 10 days.

2, experimental method: 30min after the intervention of the 10d medicine, 30 mu L of dimethylbenzene is coated on two sides of the right ear of the mouse, the left ear is used as a control, the mouse is killed by adopting a cervical dislocation method after 30min of dimethylbenzene coating, two ears are cut off along the auricle baseline, round ear pieces are respectively punched on the same position by using a puncher with the diameter of 8mm, the mass is weighed by an electronic balance, and the swelling degree and the swelling inhibition rate are calculated. Swelling = right ear weight-left ear weight. Swelling inhibition = (average swelling degree of blank group-average swelling degree of administration group)/average swelling degree of blank group×100%.

3 experimental results: by mean ± standard deviationThe results are shown in Table 4.

Conclusion 4: the invention tests the detumescence effect of the drug by using Kunming mice, and the experimental results prove that the drug has obvious curative effect on inflammation and detumescence, and is safe and has no side effect.

Claims (9)

1. A medicament for treating osteoarthritis, characterized in that: the traditional Chinese medicine composition is prepared from 500-1500 parts of chick embryo, 100-900 parts of earthworm, 100-900 parts of fructus psoraleae, 100-900 parts of rhizoma drynariae, 10-190 parts of borneol, 200-1000 parts of gardenia, 50-800 parts of herba lycopi, 20-500 parts of radix angelicae, 10-190 parts of nux vomica, 100-900 parts of large-leaved gentian, 100-900 parts of lycopodium clavatum, 100-900 parts of herba siegesbeckiae, 100-900 parts of rhizoma smilacis glabrae and 80-650 parts of celandine according to weight parts.

2. A medicament for the treatment of osteoarthritis as claimed in claim 1, wherein: the traditional Chinese medicine composition is prepared from 900-1100 parts of chick embryo, 400-600 parts of earthworm, 400-600 parts of fructus psoraleae, 400-600 parts of rhizoma drynariae, 50-150 parts of borneol, 500-700 parts of cape jasmine, 300-500 parts of herba lycopi, 100-300 parts of radix angelicae, 50-150 parts of nux vomica, 400-600 parts of large-leaved gentian, 400-600 parts of lycopodium clavatum, 400-600 parts of herba siegesbeckiae, 400-600 parts of rhizoma smilacis glabrae and 200-400 parts of celandine according to weight parts.

3. A medicament for the treatment of osteoarthritis as claimed in claim 1 or 2, wherein: the traditional Chinese medicine composition is prepared from 1000 parts of chick embryo, 500 parts of earthworm, 500 parts of fructus psoraleae, 500 parts of rhizoma drynariae, 100 parts of borneol, 600 parts of gardenia, 400 parts of herba lycopi, 200 parts of radix angelicae, 100 parts of nux vomica, 500 parts of large-leaved gentian, 500 parts of lycopodium clavatum, 500 parts of herba siegesbeckiae, 500 parts of glabrous greenbrier rhizome and 300 parts of celandine according to the weight parts.

4. A process for preparing a medicament for the treatment of osteoarthritis as claimed in claims 1 to 3, characterized in that: all the medicinal materials are weighed according to the proportion, processed according to the conventional preparation method, combined with pharmaceutically acceptable auxiliary materials and matrixes in the medicine, and prepared into corresponding pharmaceutical preparations.

5. The method for preparing the medicine for treating osteoarthritis as claimed in claim 4, wherein: weighing the medicinal materials according to the proportion of the formula, pulverizing the medicinal materials except borneol into coarse powder, processing according to a conventional preparation method, refining into paste, adding borneol fine powder, uniformly stirring and coating on a substrate to prepare a corresponding medicinal preparation.

6. The method for preparing the medicine for treating osteoarthritis as claimed in claim 5, wherein: the pharmaceutical preparation comprises plaster, rubber plaster and gel plaster.

7. The method for preparing the medicine for treating osteoarthritis as claimed in claim 6, wherein: the plaster is prepared by the following steps: weighing all the medicinal materials except the ice flakes according to the prescription proportion, and crushing into coarse powder; adding coarse powder of medicinal materials except Borneolum Syntheticum after slightly heating 2 times of oleum Sesami, heating and stirring until the medicinal materials are fried to brown on the surface and brown in the interior; the temperature reaches 220 ℃, the dregs are fished out after the frying, and the oil after the dregs are removed is medicinal oil; continuously cooking the medicinal oil until the temperature of the oil rises to 320 ℃, and cooking with medium fire until a small amount of medicinal oil is dropped into water and is not scattered into beads; after the medicinal oil is refined, the red lead with the amount of 50% is prepared from the medicinal oil by removing fire, stirring is carried out while adding, the medicinal oil is stirred into a viscous paste, the plaster is not sticky, and the plaster is obtained by continuous wire drawing; soaking in cold water to remove toxic substances, changing water every day, and continuously for seven days; placing the plaster mass in a container, melting on slow fire, adding Borneolum Syntheticum fine powder according to the prescription proportion, and uniformly coating on plaster cloth.

8. The method for preparing the medicine for treating osteoarthritis as claimed in claim 6, wherein: the rubber paste is prepared by the following steps: weighing all the medicinal materials except the deicer slices according to the prescription proportion, soaking the medicinal materials with 6 times of 50% ethanol for 0.5h, carrying out reflux extraction for 2 times at 80 ℃ for 2 hours each time, mixing the two extracting solutions, uniformly mixing, concentrating into 80% of the original medicinal liquid, filtering while the medicinal liquid is hot, and concentrating the filtrate into an extract with relative density of 1.20-1.30 at 80 ℃; taking 10 times of raw rubber, drying at 50 ℃, cutting into strips, plasticating into net-shaped sheets in a rubber mixing machine, spreading for open cooling, eliminating static electricity, soaking in a proper amount of gasoline for 20 hours, moving the gel after swelling completely into gel, stirring for 3 hours, adding 2% of vaseline, 5% of lanolin, 10% of zinc oxide and 1% of rosin into the gel to prepare a matrix, adding the human extract and borneol which is prepared into fine powder according to the prescription proportion, stirring uniformly, coating the gel, quick drying, lining, cutting and packaging to obtain the finished product.

9. The method for preparing the medicine for treating osteoarthritis as claimed in claim 6, wherein: the gel paste is prepared by the following steps: weighing all the medicinal materials except the deicer slices according to the prescription proportion, soaking the medicinal materials with 8 times of 50% ethanol for 0.5h, carrying out reflux extraction for 3 times at 80 ℃ for 1 hour each time, mixing the three extracting solutions, uniformly mixing, concentrating the mixed solution into 80% of the original medicinal liquid, filtering while the mixed solution is hot, concentrating the filtrate into an extract with relative density of 1.20-1.30 at 80 ℃, and adding water with the preparation amount of 50% into the extract for later use; mixing sodium polyacrylate 20g, gelatin 10g and silica gel micropowder 2g, adding glycerol 30g, pouring the mixture into the extract, adding Borneolum Syntheticum fine powder according to the prescription, uniformly coating on non-woven fabric to a thickness of about 1.5mm, oven drying 0.5h, curing at room temperature 48h, covering with backing layer, and sealing and packaging.