CN114467405A - Method for restoring saline-alkali soil by culturing indigenous microorganisms and application thereof - Google Patents
Method for restoring saline-alkali soil by culturing indigenous microorganisms and application thereof Download PDFInfo
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- CN114467405A CN114467405A CN202210123992.1A CN202210123992A CN114467405A CN 114467405 A CN114467405 A CN 114467405A CN 202210123992 A CN202210123992 A CN 202210123992A CN 114467405 A CN114467405 A CN 114467405A
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- 239000002689 soil Substances 0.000 title claims abstract description 134
- 239000003513 alkali Substances 0.000 title claims abstract description 97
- 244000005700 microbiome Species 0.000 title claims abstract description 77
- 238000000034 method Methods 0.000 title claims abstract description 62
- 238000012258 culturing Methods 0.000 title claims abstract description 39
- 239000003623 enhancer Substances 0.000 claims abstract description 42
- 235000016709 nutrition Nutrition 0.000 claims abstract description 38
- 230000035764 nutrition Effects 0.000 claims abstract description 38
- 230000003321 amplification Effects 0.000 claims abstract description 18
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 18
- 239000000725 suspension Substances 0.000 claims abstract description 18
- 238000005507 spraying Methods 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 13
- 150000003839 salts Chemical class 0.000 claims abstract description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 38
- 241000209094 Oryza Species 0.000 claims description 23
- 235000007164 Oryza sativa Nutrition 0.000 claims description 23
- 235000009566 rice Nutrition 0.000 claims description 23
- 238000003756 stirring Methods 0.000 claims description 23
- 240000008042 Zea mays Species 0.000 claims description 22
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 22
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 22
- 235000005822 corn Nutrition 0.000 claims description 22
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 19
- 239000004202 carbamide Substances 0.000 claims description 19
- 235000013877 carbamide Nutrition 0.000 claims description 19
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 19
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 19
- 235000013379 molasses Nutrition 0.000 claims description 19
- 235000002639 sodium chloride Nutrition 0.000 claims description 19
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 18
- 241000209046 Pennisetum Species 0.000 claims description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 15
- 238000013138 pruning Methods 0.000 claims description 15
- 239000011780 sodium chloride Substances 0.000 claims description 12
- 235000015872 dietary supplement Nutrition 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 10
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 10
- 238000005070 sampling Methods 0.000 claims description 10
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 9
- 229910052757 nitrogen Inorganic materials 0.000 claims description 9
- 239000002904 solvent Substances 0.000 claims description 9
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 8
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 7
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 5
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 238000009966 trimming Methods 0.000 claims description 5
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 4
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 4
- 235000019270 ammonium chloride Nutrition 0.000 claims description 4
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 4
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 4
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 4
- 235000011151 potassium sulphates Nutrition 0.000 claims description 4
- 230000008439 repair process Effects 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 235000011148 calcium chloride Nutrition 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 229940099596 manganese sulfate Drugs 0.000 claims description 2
- 239000011702 manganese sulphate Substances 0.000 claims description 2
- 235000007079 manganese sulphate Nutrition 0.000 claims description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 2
- 238000009331 sowing Methods 0.000 claims 1
- 238000011065 in-situ storage Methods 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 15
- 230000000052 comparative effect Effects 0.000 description 11
- 238000001514 detection method Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 244000025254 Cannabis sativa Species 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 7
- 239000008367 deionised water Substances 0.000 description 6
- 229910021641 deionized water Inorganic materials 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000005067 remediation Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000003895 organic fertilizer Substances 0.000 description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- 230000007480 spreading Effects 0.000 description 3
- 238000003892 spreading Methods 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000005341 cation exchange Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 244000130556 Pennisetum purpureum Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011066 ex-situ storage Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000010440 gypsum Substances 0.000 description 1
- 229910052602 gypsum Inorganic materials 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000013433 optimization analysis Methods 0.000 description 1
- 238000012803 optimization experiment Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002681 soil colloid Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001550 time effect Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Chemical & Material Sciences (AREA)
- Soil Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention provides a method for restoring saline-alkali soil by culturing indigenous microorganisms and an application thereof, wherein the method for restoring the saline-alkali soil by culturing the indigenous microorganisms comprises the following steps: (1) mixing the saline-alkali soil suspension with a nutrition enhancer, and culturing to obtain an indigenous microorganism amplification solution; (2) and (3) spraying a nutrition enhancer and the indigenous microorganism amplification liquid obtained in the step (1) to the saline-alkali soil. The method for restoring the saline-alkali soil adopts a mode of directly adding the saline-alkali soil for in-situ restoration, does not need to add external microorganisms, has simple operation flow, and can quickly reduce the conductivity and the total amount of water-soluble salt in the saline-alkali soil.
Description
Technical Field
The invention belongs to the technical field of environmental microorganisms, and particularly relates to a method for repairing saline-alkali soil by culturing indigenous microorganisms and application thereof.
Background
The formation reasons of soil salinization mainly include primary salinization and secondary salinization, which cause the content of soluble salt in soil to be large and are not beneficial to the normal growth of crops. With the increasing severity of land salinization, the available cultivated land area is continuously squeezed, and the agricultural sustainable development and the food safety of China are severely restricted, so that the comprehensive treatment of the saline-alkali soil becomes an irreparable important task.
At present, the saline-alkali soil remediation mainly comprises an engineering scheme, a chemical scheme and a biological scheme. The engineering scheme mainly comprises the operations of leaching, deep ploughing, concealed conduit salt elimination and the like, but a large amount of manpower, material resources and financial resources are consumed, and the method is not suitable for large-area popularization and use. The research of improving the saline-alkali soil by using gypsum is the most mature in the chemical scheme, and the soil structure is improved and the pH value is adjusted by changing the adsorbability cation composition of the saline-alkali soil colloid, so that the effect of improving the soil is achieved, but the effect is unstable, and new risks such as pollution are easily introduced. Biological measures are currently recognized as the most effective and safe measures, and the soil quality is improved by planting salt-tolerant plants or culturing microorganisms.
CN104830915A discloses a method for preparing alcohol by using a straw molasses mixture and improving saline-alkali soil by using vinasse. The vinasse contains a large amount of vitamins and minerals, is used for saline-alkali soil, can reduce the pH value of the saline-alkali soil, effectively improves the fertility of the soil, and promotes the growth of crops. But the method has a limit on reducing the pH value of the saline-alkali soil.
CN105732221A discloses a microbial organic fertilizer with saline-alkali soil improvement effect, which comprises the following components: crop straws, vinasse, a microorganism mixed microbial inoculum and corn steep liquor. The microbial organic fertilizer has common used raw materials, the preparation method is simple and feasible, and the water-retaining property of soil can be enhanced, and the growth of plant root systems can be promoted. However, the introduction of exogenous microorganisms (i.e. mixed microbial inoculum) in the microbial organic fertilizer can increase the risk of ecological crisis, and the competitive relationship between the exogenous microorganisms and indigenous microorganisms can reduce the efficacy of the exogenous microorganisms to a certain extent.
Based on the research, it can be seen that the saline-alkali soil is widely repaired by adopting biological measures at present, but the saline-alkali soil repairing efficacy still has certain limitation, and the introduction of exogenous microorganisms can cause risks. Therefore, finding a method for repairing saline-alkali soil by culturing indigenous microorganisms is a problem which needs to be solved at present.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a method for repairing saline-alkali soil by culturing indigenous microorganisms and application thereof. The method for repairing the saline-alkali soil by culturing the indigenous microorganisms has the advantages of simple process and quick response time, avoids ecological pollution caused by introducing exogenous microorganisms, and can effectively improve the repairing efficiency of the saline-alkali soil.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a method for repairing saline-alkali soil by culturing indigenous microorganisms, which comprises the following steps:
(1) mixing the saline-alkali soil suspension with a nutrition enhancer, and culturing to obtain an indigenous microorganism amplification solution;
(2) and (3) spraying a nutrition enhancer and the indigenous microorganism amplification liquid obtained in the step (1) to the saline-alkali soil.
The invention utilizes the nutrient supplement and the indigenous microorganism amplification liquid, can quickly activate indigenous microorganisms in different positions and amplify the population quantity of the indigenous microorganisms, is supplemented with the substances such as the pennisetum hydridum, the rice bran, the garden pruners and the like, can be applied to the saline-alkali soil after ploughing, has simple process and quick effect, avoids the ecological pollution caused by introducing exogenous microorganisms, and can effectively improve the remediation efficiency of the saline-alkali soil.
In the invention, the saline-alkali soil suspension is prepared by a preparation method comprising the following steps:
sampling saline-alkali soil, adding a solvent, and mixing to obtain the saline-alkali soil suspension.
Preferably, the solvent comprises water.
Preferably, the ratio of the sampling mass of the saline-alkali soil to the volume of the solvent is 50-200 g/L.
The concentration of the active component is 50-200g/L, and can be 50g/L, 60g/L, 70g/L, 80g/L, 90g/L, 100g/L, 110g/L, 120g/L, 130g/L, 140g/L, 150g/L, 160g/L, 170g/L, 180g/L, 190g/L or 200g/L, and the like.
Preferably, the mixing mode is stirring, the stirring time is 20-40min, and the stirring speed is 50-100 r/min.
The time period of 20-40min can be 20min, 22min, 24min, 26min, 28min, 30min, 32min, 34min, 36min, 38min or 40 min.
The speed of 50-100r/min can be 50r/min, 55r/min, 60r/min, 65r/min, 70r/min, 75r/min, 80r/min, 85r/min, 90r/min, 95r/min or 100r/min, etc.
Other point values within the above range can be selected, and are not described in detail herein.
In the present invention, the nutrition enhancer includes a carbon source, a nitrogen source and inorganic salts.
Preferably, the carbon source comprises any one of glucose, maltose, sucrose or molasses or a combination of at least two thereof, preferably molasses.
The combination of the at least two can be a combination of glucose and maltose or a combination of maltose and sucrose, and the like, and any other combination modes can be selected, so that the details are not repeated.
The molasses is preferably used as the carbon source in the invention, because the molasses is used as a byproduct for sugar production and is low in price, and the carbon source optimization shows that the action of the molasses is longer in time effect than that of other carbon sources.
Preferably, the nitrogen source comprises any one of yeast powder, ammonium chloride, corn steep liquor or urea or a combination of at least two of the two, preferably the combination of the corn steep liquor and the urea.
The combination of at least two of the above-mentioned materials can be the combination of yeast powder and ammonium chloride or the combination of ammonium chloride and corn steep liquor, etc., and any other combination modes can be selected, and are not described in detail herein.
The combination of corn steep liquor and urea is preferably used as the nitrogen source in the invention, because the combination of corn steep liquor and urea is found to quickly activate indigenous microorganisms and continuously provide the nitrogen source through nitrogen source optimization experiments.
Preferably, the inorganic salt comprises any one of sodium chloride, potassium sulfate, calcium chloride, manganese sulfate, monopotassium phosphate or magnesium sulfate or a combination of at least two of the above, preferably monopotassium phosphate and magnesium sulfate.
The combination of the at least two compounds can be a combination of sodium chloride and potassium sulfate or a combination of potassium sulfate and calcium chloride, and the like, and any combination mode of the rest compounds can be selected, so that the details are not repeated.
The combination of potassium dihydrogen phosphate and magnesium sulfate is preferably used as the inorganic salt in the invention, because the potassium dihydrogen phosphate and the magnesium sulfate are found to be significant factors after response surface optimization analysis.
Preferably, the nutrient supplement includes molasses, urea, corn steep liquor, dipotassium hydrogen phosphate and magnesium sulfate.
Preferably, the nutrition enhancer comprises, by concentration: 20-30g/L of molasses, 5-10g/L of urea, 10-20g/L of corn steep liquor, 3-6g/L of dipotassium phosphate and 1-5g/L of magnesium sulfate.
The concentration of the active ingredient is 20-30g/L, and can be 20g/L, 21g/L, 22g/L, 23g/L, 24g/L, 25g/L, 26g/L, 27g/L, 28g/L, 29g/L or 30g/L, and the like.
The concentration of the above-mentioned 5-10g/L may be 5g/L, 5.5g/L, 6g/L, 6.5g/L, 7g/L, 7.5g/L, 8g/L, 8.5g/L, 9g/L, 9.5g/L or 10g/L, etc.
The concentration of the 10-20g/L can be 10g/L, 11g/L, 12g/L, 13g/L, 14g/L, 15g/L, 16g/L, 17g/L, 18g/L, 19g/L or 20g/L, etc.
The 3-6g/L can be 3g/L, 3.2g/L, 3.4g/L, 3.6g/L, 3.8g/L, 4.0g/L, 4.2g/L, 4.4g/L, 4.6g/L, 4.8g/L, 5.0g/L, 5.2g/L, 5.4g/L, 5.6g/L, 5.8g/L or 6.0g/L, etc.
The 1-5g/L can be 1g/L, 1.5g/L, 2g/L, 2.5g/L, 3g/L, 3.5g/L, 4g/L, 4.5g/L or 5g/L, etc.
Preferably, the pH value of the nutrition enhancer is 5.0-9.0.
The above-mentioned 5.0-9.0, may be 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 or 9.0, etc.
The present invention limits the pH value of the nutrition enhancer to 5.0-9.0 because the nutrition enhancer in this range is suitable for the growth and metabolism of microorganisms and does not have a great influence on the pH of soil after being added.
Other point values within the above range can be selected, and are not described in detail herein.
In the invention, the volume ratio of the saline-alkali soil suspension liquid in the step (1) to the nutrition enhancer is 1 (8-25), preferably 1 (10-20).
The 1 (8-25) can be 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:21, 1:22, 1:23, 1:24 or 1:25, and the like.
The volume ratio of the saline-alkali soil suspension to the nutrition enhancer is preferably 1 (8-25), because the ratio can meet the survival condition of indigenous microorganisms. Further preferred is 1 (10-20) because this ratio is suitable for growth and reproduction of indigenous microorganisms.
In the invention, the saline-alkali soil suspension and the nutrition enhancer in the step (1) are mixed by stirring, the stirring frequency is 1.5-3 h/time, the stirring speed is 200-400r/min, and the time of each stirring is 5-15 min.
The 1.5-3 h/time can be 1.5 h/time, 1.7 h/time, 2.0 h/time, 2.2 h/time, 2.4 h/time, 2.6 h/time, 2.8 h/time or 3 h/time and the like.
The 200-400r/min can be 200r/min, 220r/min, 240r/min, 260r/min, 280r/min, 300r/min, 320r/min, 340r/min, 360r/min, 380r/min or 400r/min, etc.
The 5-15min can be 5min, 6min, 7min, 8min, 9min, 10min, 11min, 12min, 13min, 14min or 15 min.
Preferably, the culturing period is 1 to 5 days.
The 1-5 days can be 1 day, 2 days, 3 days, 4 days or 5 days, etc.
Other point values within the above range can be selected, and are not described in detail herein.
In the invention, the volume ratio of the nutrition enhancer and the indigenous microorganism amplification liquid used in the spraying in the step (2) is (0.15-0.35):1, preferably (0.2-0.3): 1.
The volume ratio of the nutrient enhancer to the indigenous microorganism amplification liquid is preferably (0.15-0.35):1, so that the survival conditions of the microorganisms after the nutrient enhancer is sprayed to soil can be met. Further, it is preferably (0.2-0.3):1, because the optimum conditions for growth and propagation of microorganisms can be obtained after spraying the soil at this ratio.
The ratio of (0.15-0.35):1 may be 0.15:1, 0.17:1, 0.19:1, 0.21:1, 0.23:1, 0.25:1, 0.27:1, 0.29:1, 0.31:1, 0.33:1 or 0.35: 1.
Preferably, the spraying amount of the step (2) is 0.6-1.0L/m2。
The 0.6-1.0L/m2May be 0.6L/m2、0.65L/m2、0.7L/m2、0.75L/m2、0.8L/m2、0.85L/m2、0.9L/m2Or 1.0L/m2And the like.
Other point values within the above range can be selected, and are not described in detail herein.
Preferably, after the spraying in the step (2), any one or a combination of at least two of the Jujun grass, the rice bran or the garden pruning is/are sown into the saline-alkali soil and ploughed.
The combination of at least two of the plants can be the combination of Jujun grass and rice bran or the combination of rice bran and garden pruning objects, and the like, and the combination mode of the rest plants can be selected at will, and is not repeated.
According to the invention, the pennisetum hydridum, the rice bran, the garden pruning material and other substances are supplemented, and the ploughed pennisetum hydridum is applied to the saline-alkali soil, so that the saline-alkali soil remediation efficiency can be effectively improved.
In the invention, the Jujun grass and the garden pruning objects need to be crushed by a crusher before being sowed.
Preferably, the pulverizing to pennisetum sinese and the garden clippings ends when the length is 0-5 cm.
The 0-5cm, excluding 0cm, may be 0.5cm, 1cm, 1.5cm, 2cm, 2.5cm, 3cm, 3.5cm, 4cm, 4.5cm, 5cm, or the like.
In the invention, the mass ratio of the pennisetum hydridum to the rice bran to the garden pruning product is 1 (1-2) to 1-2.
The (1-2) may be 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9 or 2.
Preferably, the total mass of the pennisetum sinese roxb, the rice bran and the garden pruning is 1-5kg/m when the pennisetum sinese roxb, the rice bran and the garden pruning are sown2。
The 1-5kg/m2May be 1kg/m2、1.5kg/m2、2kg/m2、2.5kg/m2、3kg/m2、3.5kg/m2、4kg/m2、4.5kg/m2Or 5kg/m2And the like.
Other point values within the above range can be selected, and are not described in detail herein.
In the invention, the plowing frequency is 2-4 times.
The number of the 2-4 times can be 2, 3 or 4, and the like.
Preferably, the depth of the plowing is 20-30 cm.
The 20-30cm can be 20cm, 21cm, 22cm, 23cm, 24cm, 25cm, 26cm, 27cm, 28cm, 29cm or 30cm, etc.
Other point values within the above range can be selected, and are not described in detail herein.
In a second aspect, the invention provides an application of the method for repairing saline-alkali soil by culturing the indigenous microorganisms according to the first aspect in repairing the saline-alkali soil.
Compared with the prior art, the invention has the following beneficial effects:
the invention utilizes the nutrient enhancer and the indigenous microorganism amplification liquid with a specific formula, can quickly activate indigenous microorganisms in different positions and amplify the population quantity of the indigenous microorganisms, is supplemented with the substances such as pennisetum purpureum, rice bran, garden trimmings and the like, can be applied to the saline-alkali soil after ploughing, has simple process and quick effect, avoids ecological pollution caused by introducing exogenous microorganisms, and can effectively improve the repair efficiency of the saline-alkali soil.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
Detection points 1-3 of saline-alkali soil related to the following are all land blocks near eighteen paths of the Bohai sea in a new coastal region in Tianjin.
The following examples or comparative examples are shown below for the corresponding materials and starting materials as purchased from the following sources:
wherein the molasses is purchased from general New materials, Inc. of Qingzhou city; corn steep liquor is purchased from bio-technology limited, Shandong; jujun grass is from Tianjin ring microbiological technology limited; the garden trimmings are from Tianjin ring microbiological technology limited; the rice bran is purchased from bio-technologies limited, Shandong. The remaining materials and starting materials are not specifically described and are commercially available from other sources.
Example 1
The embodiment provides a method for repairing saline-alkali soil by culturing indigenous microorganisms, which comprises the following steps:
(1) respectively sampling 500g of soil at detection points 1, 2 and 3 of the saline-alkali soil, wherein the sampling depth is 0-50cm, fully and uniformly mixing, weighing 500g of the uniformly mixed soil, placing the uniformly mixed soil in a barrel with a stirrer, adding 5L of deionized water, stirring for 30min at a speed of 80r/min, and discarding bottom layer sludge to obtain saline-alkali soil suspension;
(2) adding 4.5L of the soil suspension obtained in the step (1) into a stirring barrel, then adding 450L of deionized water, adding 45L of nutrition enhancer, stirring for 10min every 2h, and continuously culturing for 3d to obtain 495.5L of indigenous microorganism amplification liquid;
(3) adding 100L of nutrition enhancer into 495.5L of indigenous microorganism amplification liquid in the step (2), and uniformly spraying to 750m by pipeline spraying facilities2The application amount of the soil at the detection point 1 of the saline-alkali soil is 0.8L/m2；
(4) Pulverizing 250kg of Jujun grass and 250kg of garden pruning material to 0-5cm, adding 250kg of rice bran, mixing, and spreading to 750m2In the soil of the detection point 1 of the saline-alkali soil, a ploughing machine is used for ploughing for 3 times according to a cross ploughing method, the ploughing depth is 20cm, and the test is carried out after 30 days;
the formula of the nutrition enhancer is as follows: 25g/L of molasses, 7g/L of urea, 15g/L of corn steep liquor, 4g/L of dipotassium phosphate, 3g/L of magnesium sulfate, water as a solvent and 7.0 of pH.
Example 2
The embodiment provides a method for cultivating indigenous microorganisms to restore saline-alkali soil, which comprises the following steps:
(1) respectively sampling 500g of soil at detection points 1, 2 and 3 of the saline-alkali soil, wherein the sampling depth is 0-50cm, fully and uniformly mixing, weighing 500g of the uniformly mixed soil, placing the uniformly mixed soil in a barrel with a stirrer, adding 8L of deionized water, stirring for 40min at 60r/min, and discarding bottom layer sludge to obtain saline-alkali soil suspension;
(2) adding 5L of the soil suspension obtained in the step (1) into a stirring barrel, then adding 500L of deionized water, adding 75L of nutrition enhancer, stirring for 8min every 1.5h, and continuously culturing for 5d to obtain 580L of indigenous microorganism amplification solution;
(3) adding 145L of nutrition enhancer into 580L of indigenous microorganism amplification liquid in the step (2), and uniformly spraying the nutrition enhancer to 750m through a pipeline spraying facility2The application amount in the soil of the detection point 2 of the saline-alkali soil is 1.0L/m2；
(4) Pulverizing 250kg of Jujun grass and 300kg of garden pruning material to 0-5cm, adding 350kg of rice bran, mixing, and spreading to 750m2In the soil at the detection point 2 of the saline-alkali soil, a ploughing machine is used for ploughing for 4 times according to a cross ploughing method, the ploughing depth is 20cm, and the test is carried out after 30 days;
the formula of the nutrition enhancer is as follows: 20g/L of molasses, 5g/L of urea, 10g/L of corn steep liquor, 3g/L of dipotassium phosphate, 1g/L of magnesium sulfate, water as a solvent and 8.0 of pH.
Example 3
The embodiment provides a method for repairing saline-alkali soil by culturing indigenous microorganisms, which comprises the following steps:
(1) respectively sampling 600g of soil at detection points 1, 2 and 3 of the saline-alkali soil, wherein the sampling depth is 0-50cm, fully and uniformly mixing, weighing 600g of the uniformly mixed soil, placing the uniformly mixed soil in a barrel with a stirrer, adding 4L of deionized water, stirring for 30min at 100r/min, and discarding bottom layer sludge to obtain saline-alkali soil suspension;
(2) adding 20L of the soil suspension obtained in the step (1) into a stirring barrel, then adding 1800L of deionized water, adding 360L of nutrition enhancer, stirring for 15min every 3h, and continuously culturing for 2 days to obtain 2180L of indigenous microorganism amplification liquid;
(3) adding 650L of nutrition enhancer into 2180L of the indigenous microorganism amplification liquid in the step (2), and uniformly spraying to 750m by a pipeline spraying facility2The application amount of the soil at the detection point 3 of the saline-alkali soil is 0.7L/m2；
(4) Pulverizing 250kg of Jujun grass and 400kg of garden pruning material to 0-5cm, adding 300kg of rice bran, mixing, and spreading to 750m2In the soil at the detection point 3 of the saline-alkali soil, a ploughing machine is used for ploughing for 2 times according to a cross ploughing method, the ploughing depth is 25cm, and the test is carried out after 30 days;
the formula of the nutrition enhancer is as follows: 30g/L of molasses, 10g/L of urea, 20g/L of corn steep liquor, 6g/L of dipotassium phosphate, 5g/L of magnesium sulfate, water as a solvent and 6.0 of pH.
Example 4
This example provides a method for remediating saline and alkaline land soil by culturing indigenous microorganisms, differing from example 1 only in that the nutrient supplement does not include molasses, corn steep liquor and urea, the reduced mass thereof is supplemented with potassium dihydrogen phosphate and magnesium sulfate, the mass ratio of potassium dihydrogen phosphate to magnesium sulfate remains the same, and the remaining parameters are in accordance with example 1. Experimental methods refer to example 1.
Example 5
This example provides a method for remediating saline and alkaline land soil by culturing indigenous microorganisms, differing from example 1 only in that the nutrient supplement does not comprise monopotassium phosphate and magnesium sulfate, the reduced mass thereof is supplemented by molasses, corn steep liquor and urea, the mass ratio of molasses, corn steep liquor and urea remains the same, and the remaining parameters are in accordance with example 1. Experimental methods refer to example 1.
Example 6
This example provides a method for remediating saline and alkaline land soil by cultivating indigenous microorganisms, differing from example 1 only in that the nutrient supplement does not include urea, the reduced mass of which is complemented by corn steep liquor, and the remaining parameters being in accordance with example 1. Experimental methods refer to example 1.
Example 7
This example provides a method for remediating saline and alkaline land soil by culturing indigenous microorganisms, which differs from example 1 only in that the nutrient supplement does not include corn steep liquor, the reduced mass of which is complemented by urea, and the remaining parameters are in accordance with example 1. Experimental methods refer to example 1.
Example 8
This example provides a method for remediating saline and alkaline land soil by culturing indigenous microorganisms, differing from example 1 only in that the nutrient supplement does not include dipotassium hydrogen phosphate, the reduced mass of which is complemented by magnesium sulfate, the remaining parameters remaining in accordance with example 1. Experimental methods refer to example 1.
Example 9
This example provides a method for remediating saline and alkaline land soil by culturing indigenous microorganisms, differing from example 1 only in that the nutrient supplement does not include magnesium sulfate, the reduced mass of which is complemented by dipotassium hydrogen phosphate, the remaining parameters remaining in accordance with example 1. Experimental methods refer to example 1.
Example 10
The present example provides a method for repairing saline-alkali soil by culturing indigenous microorganisms, which is different from example 1 only in that the addition amount of the nutrition enhancer in step (2) is 20L, and the rest parameters are consistent with those in example 1. Experimental methods refer to example 1.
Example 11
The present example provides a method for repairing saline-alkali soil by culturing indigenous microorganisms, which is different from example 1 only in that the addition amount of the nutrition enhancer in step (2) is 135L, and the rest parameters are consistent with those in example 1. Experimental methods refer to example 1.
Example 12
The present example provides a method for repairing saline-alkali soil by culturing indigenous microorganisms, which is different from example 1 only in that the addition amount of the nutrition enhancer in step (3) is 30L, and the rest parameters are consistent with those in example 1. Experimental methods refer to example 1.
Example 13
The present example provides a method for repairing saline-alkali soil by culturing indigenous microorganisms, which is different from example 1 only in that the addition amount of the nutrition enhancer in step (3) is 250L, and the rest parameters are consistent with those in example 1. Experimental methods refer to example 1.
Example 14
The comparative example provides a method for restoring saline-alkali soil, which is different from the method in example 1 only in that Jujun grass, garden trimmings and rice bran are not added in the step (4), and the rest parameters are consistent with those in example 1. Experimental methods refer to example 1.
Example 15
The comparative example provides a method for restoring saline-alkali soil, which is different from the method in example 1 only in that rice bran is not added in the step (4), the reduced mass of the rice bran is supplemented by the pennisetum hydridum and the garden pruning, the mass ratio of the pennisetum hydridum to the garden pruning remains unchanged, and the rest parameters are consistent with the method in example 1. Experimental methods refer to example 1.
Example 16
This comparative example provides a method for remediating saline and alkaline land soil, which differs from example 1 only in that step (4) does not add pennisetum sinese and garden clippings, the reduced mass is complemented by rice bran, and the remaining parameters are in accordance with example 1. Experimental methods refer to example 1.
Comparative example 1
The comparative example provides a method for repairing saline-alkali soil, and the method is only different from the method in the example 1 in that no nutrition enhancer is added in the step (2), and the other parameters are consistent with those in the example 1. Experimental methods refer to example 1.
Comparative example 2
The comparative example provides a method for repairing saline-alkali soil, and the method is only different from the method in the example 1 in that no nutrition enhancer is added in the step (3), and other parameters are consistent with those in the example 1. Experimental methods refer to example 1.
Test example 1
In the test example, the saline-alkali soil after 30 days of the method for repairing the saline-alkali soil by using the examples 1 to 16 and the comparative examples 1 to 2 was sampled (with a depth of 0 to 50cm) and used as an experimental group, and the saline-alkali soil which was not repaired in each group was sampled (with a depth of 0 to 50cm) and used as a control group, and the pH, the conductivity, the total amount of water-soluble salts, the cation exchange amount and the organic matter of the soil were respectively detected. The specific test method is as follows:
wherein the pH is measured by the method HJ 962-; the conductivity was tested by the method HJ 802-2016; the total amount of the water-soluble salt is tested according to the NY/T1121.16-2006 method; the cation exchange capacity is tested according to the method HJ 889-2017; organic matter was tested according to NY/T1121.6-2006 method.
The statistics of the test results are shown in table 1 below:
TABLE 1
From the above table data, it can be seen that: the method for cultivating the indigenous microorganisms to repair the saline-alkali soil can effectively reduce the salt content of the saline-alkali soil, can increase organic matters in the soil, and is beneficial to the growth of crops. Among them, the data of examples 4 to 5 show that: the formula of the nutrition enhancer can influence the effect of repairing the saline-alkali soil, and when a carbon source, a nitrogen source and inorganic salt exist simultaneously, the effect of repairing the saline-alkali soil can be synergistically improved. From the data of examples 6-9, it can be seen that: wherein the corn steep liquor and the urea are matched for use, the function of a nitrogen source can be synergistically improved, and the potassium dihydrogen phosphate and the magnesium sulfate are matched for use, and the function of inorganic salt can be synergistically improved. From the data of examples 10 to 13, it is known that the addition amount of the nutrient supplement affects the effect of saline-alkali soil remediation. From the data of comparative examples 14-16, it is clear that the addition of pennisetum hydridum, garden clippings and rice bran as a combination has an effect on the remediation of saline and alkaline land. The data of the comparative examples 1-2 show that the addition of the nutrition enhancer has obvious influence on the effect of repairing the saline-alkali soil.
In conclusion, the nutrient enhancer and the indigenous microorganism amplification liquid with a specific formula are utilized, the indigenous microorganisms can be activated ex situ and the population quantity of the indigenous microorganisms can be amplified, the indigenous microorganisms are supplemented with the substances such as the Jujun grass, the rice bran, the garden pruning substances and the like, and the obtained product is applied to the saline-alkali soil after ploughing.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A method for repairing saline-alkali soil by culturing indigenous microorganisms is characterized by comprising the following steps:
(1) mixing the saline-alkali soil suspension with a nutrition enhancer, and culturing to obtain an indigenous microorganism amplification solution;
(2) and (3) spraying a nutrition enhancer and the indigenous microorganism amplification liquid obtained in the step (1) to the saline-alkali soil.
2. The method for remediating saline-alkali soil by culturing indigenous microorganisms according to claim 1, wherein the saline-alkali soil suspension is prepared by a preparation method comprising the following steps:
sampling saline-alkali soil, adding a solvent, and mixing to obtain a saline-alkali soil suspension;
preferably, the solvent comprises water;
preferably, the ratio of the sampling mass of the saline-alkali soil to the volume of the solvent is 50-200 g/L;
preferably, the mixing mode is stirring, the stirring time is 20-40min, and the stirring speed is 50-100 r/min.
3. The method for remediating saline and alkaline land soil by culturing indigenous microorganisms according to claim 1 or 2, wherein the nutrition enhancer comprises a carbon source, a nitrogen source and inorganic salts;
preferably, the carbon source comprises any one of glucose, maltose, sucrose or molasses or a combination of at least two thereof, preferably molasses;
preferably, the nitrogen source comprises any one or combination of at least two of yeast powder, ammonium chloride, corn steep liquor or urea, preferably the combination of the corn steep liquor and the urea;
preferably, the inorganic salt comprises any one or combination of at least two of sodium chloride, potassium sulfate, calcium chloride, manganese sulfate, potassium dihydrogen phosphate or magnesium sulfate, preferably the combination of potassium dihydrogen phosphate and magnesium sulfate;
preferably, the nutrient supplement includes molasses, urea, corn steep liquor, dipotassium hydrogen phosphate and magnesium sulfate;
preferably, the nutrition enhancer comprises, by concentration: 20-30g/L of molasses, 5-10g/L of urea, 10-20g/L of corn steep liquor, 3-6g/L of dipotassium phosphate and 1-5g/L of magnesium sulfate;
preferably, the pH value of the nutrition enhancer is 5.0-9.0.
4. The method for remediating saline-alkali soil by culturing the indigenous microorganisms to any one of claims 1 to 3, wherein the volume ratio of the saline-alkali soil suspension to the nutrition enhancer in the step (1) is 1 (8-25), preferably 1 (10-20).
5. The method for remediating saline-alkali soil by culturing indigenous microorganisms as claimed in any one of claims 1 to 4, wherein the saline-alkali soil suspension and the nutrition enhancer in the step (1) are mixed by stirring, the frequency of the stirring is 1.5 to 3 h/time, the stirring speed is 200-400r/min, and the time of each stirring is 5 to 15 min;
preferably, the culturing period is 1 to 5 days.
6. The method for remediating saline-alkali soil by culturing indigenous microorganisms according to any one of claims 1 to 5, wherein the volume ratio of the nutrient supplement to the indigenous microorganism amplification solution used in the spraying of the step (2) is (0.15-0.35):1, preferably (0.2-0.3): 1;
preferably, the spraying amount of the step (2) is 0.6-1.0L/m2。
7. The method for remediating saline-alkali soil by culturing indigenous microorganisms according to any one of claims 1 to 6, wherein the spraying of the step (2) further comprises sowing any one or a combination of at least two of pennisetum hydridum, rice bran or garden trimmings into the saline-alkali soil, and ploughing;
preferably, the pennisetum hydridum and the garden trimmings need to be crushed by a crusher before being sowed;
preferably, the pulverizing to pennisetum sinese and the garden clippings ends when the length is 0-5 cm.
8. The method for remediating saline-alkali soil by culturing indigenous microorganisms as claimed in claim 7, wherein the mass ratio of the pennisetum hydridum to the rice bran to the garden pruner is 1 (1-2) to (1-2);
preferably, the total mass of the pennisetum sinese roxb, the rice bran and the garden pruning is 1-5kg/m when the pennisetum sinese roxb, the rice bran and the garden pruning are sown2。
9. The method for remediating saline-alkali soil by culturing indigenous microorganisms according to claim 7 or 8, wherein the number of plowing is 2 to 4;
preferably, the depth of the plowing is 20-30 cm.
10. The use of the method for cultivating an indigenous microorganism to repair saline-alkali soil according to any one of claims 1-9 in the repair of saline-alkali soil.
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