CN103736725A - Soil remediation method and biological agent for same, and preparation method and application of biological agent - Google Patents
Soil remediation method and biological agent for same, and preparation method and application of biological agent Download PDFInfo
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- CN103736725A CN103736725A CN201410021333.2A CN201410021333A CN103736725A CN 103736725 A CN103736725 A CN 103736725A CN 201410021333 A CN201410021333 A CN 201410021333A CN 103736725 A CN103736725 A CN 103736725A
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Abstract
The invention provides a biological agent for soil remediation, a preparation method and application thereof, and a soil remediation method by using the biological agent. The biological agent for soil remediation comprises the following components in parts by weight: 0.75-5.5 parts of nocardia, 0.75-1.25 parts of lactobacillus and 0.75-2.5 parts of bacillus. The biological agent can arouse aboriginal microbes in raw soil, strengthen the biological populations in the soil, gradually lower the pH value of the soil, enhance the air permeability and porosity of the soil, reduce the heavy metal content, restore and enhance the soil activity, improve various indexes of the raw soil, and enable the remediated raw soil to conform to relevant standards for municipal landscaping planting soil.
Description
Technical field
The invention belongs to soil remediation field, be specifically related to a kind of soil remediation method and biologic product, Preparation method and use for this soil remediation method.
Background technology
Soil is one of mankind's Main Natural Resources of depending on for existence, is also the important component part of human ecological environment.Along with industry, the aggravation of municipal pollution and using of agrochemicals, heavy metal pollution of soil is day by day serious, is almost threatening each country.At present, approximately 1.5 ten thousand tons of Hg are discharged in the whole world every year on average, approximately 3,400,000 tons of Cu, approximately 5,000,000 tons of Pb, approximately 1,500 ten thousand tons of Mn, approximately 1,000,000 tons of Ni.China is subject to nearly 2,000 ten thousand hm2 of cultivated area of the heavy metal pollutions such as cadmium, arsenic, chromium, lead, accounts for 1/5 of total area under cultivation.On the other hand, occur a large amount of original soil excavations and outwards transport and topple in development of real estate and afforestation development process, cause the destruction to road, produced simultaneously discharge has produced serious impact to environmental protection and human health.Part is afforested industry and is adopted architecture sediment, not treatedly be directly used in planting technique, a large amount of uses of recommended rate of fertilizer application disunity and pesticide, accumulation has year after year caused the decline of soil vitality, soil compaction and salinization of soil are day by day serious, have directly had influence on quality and the effect of urban afforestation.
Therefore, how repairing contaminated soil, eliminate soil compaction, make salinized soil pH recover normal, recover soil vitality, is a problem demanding prompt solution.
Summary of the invention
One of object of the present invention is to provide a kind of biologic product for soil remediation.
The present invention also aims to provide a kind of preparation method of the biologic product for soil remediation.
The present invention also aims to provide the microbial composite that formed by Nocard's bacillus, lactic acid bacteria, the bacillus purposes for soil-repairing agent.
The present invention also aims to provide a kind of geobiont restorative procedure.
The invention provides a kind of biologic product for soil remediation, comprise the microbial composite being formed by Nocard's bacillus (Nocardia), lactic acid bacteria (Lactobacillus), bacillus (Bacillus), and absorption carrier, wherein in microbial composite, the weight ratio of each component is: Nocard's bacillus: lactic acid bacteria: bacillus=0.75-5.5:0.75-1.25:0.75-2.5.
Preferably, in described biologic product, in microbial composite, the weight ratio of each component is: Nocard's bacillus: lactic acid bacteria: bacillus=55:15:30.
Preferably, Nocard's bacillus described in described biologic product is nocardia asteroide (Nocardia asteroides); Lactic acid bacteria is lactobacillus acidophilus (Lactobacillus acidophilus); Bacillus is bacillus subtilis (Bacillus subtilis).
Preferably, in described biologic product, the weight ratio of microbial composite and absorption carrier is: 1:100-150.
Preferably, in described biologic product, absorption carrier is straw, as rice straw, Wheat Straw, soybean stalk, Wheat Straw, sesame stalk.
The present invention also provides the preparation method for the biologic product of soil remediation, and it comprises the following steps:
(1) respectively Nocard's bacillus, lactic acid bacteria, bacillus are inoculated in to one-level culture medium and carry out one-level cultivation, obtain first class inoculum;
(2) respectively the first class inoculum of Nocard's bacillus, lactic acid bacteria, bacillus is inoculated in to secondary medium and carries out secondary cultivation, obtain zymocyte liquid;
(3) each zymocyte liquid is mixed and makes microbial composite by following part by weight: Nocard's bacillus: lactic acid bacteria: bacillus=0.75-5.5:0.75-1.25:0.75-2.5;
(4) gained microbial composite and absorption carrier are mixed according to weight ratio 1:100-150;
Inoculum concentration 10% ~ 50% when wherein Nocard's bacillus one-level is cultivated in step (1), cultivates pH6 ~ 8,27 ~ 32 ℃ of cultivation temperature, incubation time 5 ~ 7 days; Inoculum concentration 5% ~ 20% when lactic acid bacteria one-level is cultivated, cultivates pH6 ~ 8,28 ~ 34 ℃ of cultivation temperature, incubation time 2 ~ 5 days; Inoculum concentration 5% ~ 15% when bacillus one-level is cultivated, cultivates pH6 ~ 8,28 ~ 34 ℃ of cultivation temperature, incubation time 2 ~ 3 days;
In step (2), Nocard's bacillus secondary is cultivated as ventilation and is cultivated, and inoculum concentration 20% ~ 50%, cultivates pH6 ~ 8,27 ~ 32 ℃ of cultivation temperature, incubation time 5 ~ 7 days; Lactic acid bacteria secondary is cultivated as stuffiness and is cultivated, and inoculum concentration 5% ~ 20%, cultivates pH6 ~ 8,28 ~ 34 ℃ of cultivation temperature, incubation time 2 ~ 5 days; Bacillus secondary is cultivated as ventilation and is cultivated, and inoculum concentration 5% ~ 15%, cultivates pH6 ~ 8,28 ~ 32 ℃ of cultivation temperature, incubation time 2 ~ 5 days;
In step (3), Nocard's bacillus fermented and cultured is ventilation cultivation, second class inoculum is inoculated in to 500mL seeding tank, inoculum concentration 20% ~ 50%, cultivate pH6 ~ 8,27 ~ 32 ℃ of cultivation temperature, incubation time is inoculated in 2 village fermentation tanks after 5 ~ 7 days, inoculum concentration 20% ~ 50%, cultivate pH7 ~ 8,28 ~ 36 ℃ of cultivation temperature, incubation time 7 ~ 10 days; Lactobacillus-fermented is cultivated as stuffiness cultivation, second class inoculum is inoculated in to 200L seeding tank, inoculum concentration 5% ~ 20%, cultivates pH6 ~ 8,28 ~ 32 ℃ of cultivation temperature, incubation time 2 ~ 3 days, be inoculated in afterwards 2 village fermentation tanks, inoculum concentration 5% ~ 20%, cultivates pH7 ~ 8,28 ~ 36 ℃ of cultivation temperature, incubation time 2 ~ 3 days; Fermentation of bacillus is cultivated as ventilation cultivation, second class inoculum is inoculated in to 200L seeding tank, inoculum concentration 5% ~ 15%, cultivates pH6 ~ 8,28 ~ 32 ℃ of cultivation temperature, incubation time 2 ~ 3 days, be inoculated in afterwards 2 tons of fermentation tanks, inoculum concentration 20% ~ 50%, cultivates pH7 ~ 8,28 ~ 36 ℃ of cultivation temperature, incubation time 2 ~ 3 days.
The present invention also provides the microbial composite that is comprised of Nocard's bacillus, lactic acid bacteria, the bacillus purposes for soil-repairing agent.
Preferably, in described purposes, the weight ratio of Nocard's bacillus, lactic acid bacteria, bacillus is: Nocard's bacillus: lactic acid bacteria: bacillus=0.75-5.5:0.75-1.25:0.75-2.5.
Preferably, in described purposes, the weight ratio of Nocard's bacillus, lactic acid bacteria, bacillus is: Nocard's bacillus: lactic acid bacteria: bacillus=55:15:30.
Preferably, Nocard's bacillus described in described purposes is nocardia asteroide (Nocardia asteroides); Lactic acid bacteria is lactobacillus acidophilus (Lactobacillus acidophilus); Bacillus is bacillus subtilis (Bacillus subtilis).
The present invention also provides a kind of geobiont restorative procedure, comprise the step that the biologic product for soil remediation is mixed with the soil of needs reparation, the described biologic product for soil remediation comprises the microbial composite being comprised of Nocard's bacillus, lactic acid bacteria, bacillus, and absorption carrier, wherein in microbial composite, the weight ratio of each component is: Nocard's bacillus: lactic acid bacteria: bacillus=0.75-5.5:0.75-1.25:0.75-2.5.
Preferably, in described geobiont restorative procedure, the weight ratio of each component is: Nocard's bacillus: lactic acid bacteria: bacillus=55:15:30.
Preferably, Nocard's bacillus described in described geobiont restorative procedure is nocardia asteroide (Nocardia asteroides); Lactic acid bacteria is lactobacillus acidophilus (Lactobacillus acidophilus); Bacillus is bacillus subtilis (Bacillus subtilis).
Preferably, in described geobiont restorative procedure, the weight ratio of microbial composite and absorption carrier is: 1:100-150.
Preferably, in described geobiont restorative procedure, absorption carrier is rice straw, Wheat Straw, soybean stalk, Wheat Straw, sesame stalk.
Preferably, biological prosthetic described in described geobiont restorative procedure is original position reparation.
Preferably, described geobiont restorative procedure is also included in planting plants in the soil of described mixing.
Preferably, in described geobiont restorative procedure, above-mentioned plant is at least one being selected from eggplant, Chinese cabbage, tomato.
Preferably, in described geobiont restorative procedure, before the above-mentioned plant of plantation, also comprise the step of soil humidification.
A kind of soil remediation method of the present invention and for the biologic product of this soil remediation method, the indigenous microorganism that Preparation method and use can wake original soil up, perfect the biotic population of soil, progressively reduce the pH value of soil, improve gas permeability and the gas porosity of soil, reduce content of beary metal value, recovery and raising soil vitality, the indices of repairing rear original soil is improved, meets the relevant criterion of urban landscaping planting soil.
The specific embodiment
Embodiment 1
For the biologic product of soil remediation, by the bacterial classification of following weight ratio bacterial classification, prepared: 3 parts of Nocard's bacillus; 3 parts of lactic acid bacterias; 10 parts of bacillus.15-110：15-25：15-50
Embodiment 2
For the biologic product of soil remediation, by the bacterial classification of following weight ratio bacterial classification, prepared: 3 parts of Nocard's bacillus; 5 parts of lactic acid bacterias; 3 parts of bacillus.
Embodiment 3
For the biologic product of soil remediation, by the bacterial classification of following weight ratio bacterial classification, prepared: 22 parts of Nocard's bacillus; 3 parts of lactic acid bacterias; 3 parts of bacillus.
Embodiment 4
For the biologic product of soil remediation, by the bacterial classification of following weight ratio bacterial classification, prepared: 22 parts of nocardia asteroides; 3 parts of lactobacillus acidophilus; 10 parts of bacillus subtilises.
Embodiment 4
For the biologic product of soil remediation, by the bacterial classification of following weight ratio bacterial classification, prepared: 22 parts of nocardia asteroides; 5 parts of lactobacillus acidophilus; 3 parts of bacillus subtilises.
Embodiment 5
Nocard's bacillus one-level culture medium is: 1 gram, potassium nitrate, and 20 grams of soluble starches, 0.5 gram of dipotassium hydrogen phosphate, 0.5 gram, magnesium sulfate, 0.5 gram, sodium chloride, 0.01 gram, ferrous sulfate, 20 grams, agar, distilled water is supplied 1000ml, pH7.2 ~ 7.4.Secondary medium is 0.8 gram, potassium nitrate, 8 grams of soluble starches, and 5 grams of glucose, 5 grams of yeast juices, 0.3 gram of dipotassium hydrogen phosphate, 0.2 gram, magnesium sulfate, 0.4 gram, sodium chloride, 0.05 gram, ferrous sulfate, water is supplied 1000ml, pH7.2 ~ 7.4.Fermentation medium is: glucose 1%, and yeast juice 1%, dipotassium hydrogen phosphate 0.015%, sodium chloride 0.02%, ferrous sulfate 0.003%, water is supplied 100%, pH7.2 ~ 7.5.
Lactic acid bacteria one-level culture medium is: 1 gram, potassium nitrate, and 10 grams of soluble starches, 0.5 gram of dipotassium hydrogen phosphate, 0.5 gram, magnesium sulfate, 0.5 gram, sodium chloride, 0.01 gram, ferrous sulfate, 20 grams, agar, distilled water is supplied 1000ml, pH7.2 ~ 7.4.Secondary medium is: 0.8 gram, potassium nitrate, and 8 grams of soluble starches, 5 grams of glucose, 5 grams of yeast juices, 0.3 gram of dipotassium hydrogen phosphate, 0.2 gram, magnesium sulfate, 0.4 gram, sodium chloride, 0.05 gram, ferrous sulfate, water is supplied 1000ml, pH7.2 ~ 7.4.Fermentation medium is: glucose 1%, and yeast juice 1%, dipotassium hydrogen phosphate 0.015%, sodium chloride 0.02%, ferrous sulfate 0.003%, water is supplied 100%, pH7.2 ~ 7.5.
Bacillus one-level culture medium is: beef extract 0.3%, peptone 1.0%, sodium chloride 0.5%, distilled water are settled to 1000ml, pH7.0.Secondary medium is: beef extract 1.0%, peptone 1.0%, sodium chloride 0.5%, agar 2.0%, distilled water are settled to 1000ml, pH7.0.Fermentation medium is: starch 0.3%, sucrose 0.5%, 30.8mg/Kg manganese sulfate solution 0.2%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.15%, dregs of beans 3.0%, water is supplied 100%, pH7.0.
Then according to the form below condition is produced
Each zymocyte liquid is pressed to Nocard's bacillus 55 weight portions, lactic acid bacteria 15 weight portions, bacillus 30 weight portions mixing, then mix according to weight ratio 1:150 with absorption carrier rice straw powder, obtain the biologic product for soil remediation.
Embodiment 6
According to the form below condition is produced
Each zymocyte liquid is pressed to Nocard's bacillus 55 weight portions, lactic acid bacteria 15 weight portions, bacillus 30 weight portions mixing, then mix according to weight ratio 1:150 with absorption carrier Wheat Straw powder, obtain the biologic product for soil remediation.
Embodiment 7
According to the form below condition is produced
Each zymocyte liquid is mixed by nocardia asteroide 55 weight portions, lactobacillus acidophilus 15 weight portions, bacillus subtilis 30 weight portions, then mix according to weight ratio 1:150 with absorption carrier soybean stalk powder, obtain the biologic product for soil remediation.
Test example
Soil in-situ repairing test
Biologic product prepared by test sample: embodiment 7.
Test method: test plot is divided into 8 regions, comprise 2 clear areas and 6 trial zones, 6 every 2 of trial zones are that a component is 3 groups, and every group respectively according to every mu of 1000kg(DS1000), 2000kg(DS2000), 3000kg(DS3000) use described biologic product.And with Shen enlightening Nutrition Soil in contrast.Concrete grammar is: biologic product prepared by embodiment 7 is weighed, by machinery operation, evenly puddle in test plot soil, and suitable humidification as required, after 30 days, plant; Wherein work machine can be to the lease of professional equipment company, and the day hack ability of machine is: 450-600 cube of soil, soil smashing fineness is: 5-10mm.After crop growth period finishes, get pedotheque and detect, the overall test cycle is about 5 months.
Result of the test:
The equal multi-point sampling of regional, pH value, the content of organic matter, total nutrient content are by composing Buddhist nun's test, and content of beary metal entrusts academy of agricultural sciences, Shanghai City to detect.
The test of 1.pH value
Blank group: 8.72
DS1000：8.43
DS2000：8.56
DS3000：8.71
Control group: 8.56
Each test group is after the processing less than 5 months, and compared with blank group, soil pH all shows downward trend.
2. the content of organic matter detects (weight percent hundred)
Blank group: 0.806%
DS1000：1.81%
DS2000：1.52%
DS3000：1.09%
In each test group, the content of organic matter all rises to some extent, and the most obvious with DS1000 group.
3. total nutrient content detection (mg/kg, by N, P, K summation meter)
Blank group: 0.278
DS1000：0.309
DS2000：0.362
DS3000：0.339
In each test group, total nutrient content all rises to some extent, and the most obvious with DS2000 group.
4. content of beary metal detects (mg/kg)
5. the soil texture and geobiont are observed
The original soil of pilot region is before reparation, biological variety and quantity are very low, in the repairing test stage, a large amount of various geobionts have been there are, comprising a large amount of spiders, Big Formica fusca, locust, toad, dragonfly and part earthworm etc., in soil, there is the mycelium not having in original soil, although only have 10 months repair time, the gas porosity of soil is significantly improved.
Conclusion
The present invention can wake the indigenous microorganism of original soil up for the biologic product of soil remediation, perfect the biotic population of soil, progressively reduce the pH value of soil, improve gas permeability and the gas porosity of soil, reduce content of beary metal value, recovery and raising soil vitality, the indices of repairing rear original soil is improved, and compared with national standard (GB15618-2008), all indexs all belong to and meet relevant regulations.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (10)
1. the biologic product for soil remediation, it is characterized in that comprising the microbial composite and the absorption carrier that Nocard's bacillus, lactic acid bacteria, bacillus, consist of, in wherein said microbial composite, the weight ratio of each component is: described Nocard's bacillus: described lactic acid bacteria: described bacillus=0.75-5.5:0.75-1.25:0.75-2.5.
2. the biologic product for soil remediation as claimed in claim 1, is characterized in that, in described microbial composite, the weight ratio of each component is: described Nocard's bacillus: described lactic acid bacteria: described bacillus=55:15:30.
3. the biologic product for soil remediation as claimed in claim 1 or 2, is characterized in that, described Nocard's bacillus is nocardia asteroide, and described lactic acid bacteria is lactobacillus acidophilus, and described bacillus is bacillus subtilis.
4. the biologic product for soil remediation as claimed in claim 1 or 2, it is characterized in that, the weight ratio of described microbial composite and described absorption carrier is: 1:100-150, described absorption carrier is selected from least one of rice straw, Wheat Straw, soybean stalk, Wheat Straw, sesame stalk.
5. a geobiont restorative procedure, it is characterized in that comprising the step that the biologic product for soil remediation is mixed with the soil of needs reparation, the described biologic product for soil remediation comprises the microbial composite and the absorption carrier that Nocard's bacillus, lactic acid bacteria, bacillus, consist of, and in wherein said microbial composite, the weight ratio of each component is: described Nocard's bacillus: described lactic acid bacteria: described bacillus=0.75-5.5:0.75-1.25:0.75-2.5.
6. for a preparation method for the biologic product of soil remediation, it is characterized in that comprising the following steps:
(1) respectively Nocard's bacillus, lactic acid bacteria, bacillus are inoculated in to one-level culture medium and carry out one-level cultivation, obtain first class inoculum;
(2) respectively the first class inoculum of described Nocard's bacillus, described lactic acid bacteria, described bacillus is inoculated in to secondary medium and carries out secondary cultivation, obtain zymocyte liquid;
(3) each zymocyte liquid is mixed and makes microbial composite by following part by weight: described Nocard's bacillus: described lactic acid bacteria: described bacillus=0.75-5.5:0.75-1.25:0.75-2.5;
(4) gained microbial composite and absorption carrier are mixed according to weight ratio 1:100-150.
7. the microbial composite being comprised of Nocard's bacillus, lactic acid bacteria, bacillus is for the purposes of soil-repairing agent.
8. purposes as claimed in claim 7, is characterized in that, the weight ratio of described Nocard's bacillus, described lactic acid bacteria, described bacillus is: described Nocard's bacillus: described lactic acid bacteria: described bacillus=0.75-5.5:0.75-1.25:0.75-2.5.
9. purposes as claimed in claim 8, is characterized in that, the weight ratio of described Nocard's bacillus, described lactic acid bacteria, described bacillus is: Nocard's bacillus: lactic acid bacteria: bacillus=55:15:30.
10. the purposes as described in any one in claim 7 to 9, wherein said Nocard's bacillus is nocardia asteroide; Described lactic acid bacteria is lactobacillus acidophilus; Described bacillus is bacillus subtilis.
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| CN105710122A (en) * | 2016-04-27 | 2016-06-29 | 上海大颂生物技术有限公司 | In-situ mechanical remediation method of urban landscaping planting soil |
| CN107722987A (en) * | 2017-09-12 | 2018-02-23 | 上海圣珑环境修复技术有限公司 | A kind of preparation of metal ion stabilizer and its restorative procedure to arable soil |
| CN108114976A (en) * | 2017-12-19 | 2018-06-05 | 轻工业环境保护研究所 | A kind of method of repairing polluted soil |
| CN108307945A (en) * | 2018-01-16 | 2018-07-24 | 平南县正达农业发展有限公司 | A kind of diseases prevention implantation methods of dragon fruit |
| CN110004089A (en) * | 2019-04-15 | 2019-07-12 | 微生物肥料技术研究推广中心 | The method and application of microbial bacterial agent and its rehabilitating soil |
| CN110846041A (en) * | 2019-11-15 | 2020-02-28 | 黑龙江惊哲森林食品集团有限公司 | Soybean water lactobacillus soil remediation agent and preparation method thereof |
| CN112470587A (en) * | 2020-10-30 | 2021-03-12 | 中国农业科学院草原研究所 | Method for quickly repairing surface soil of degraded grassland |
| CN114467405A (en) * | 2022-02-10 | 2022-05-13 | 自然资源部天津海水淡化与综合利用研究所 | Method for restoring saline-alkali soil by culturing indigenous microorganisms and application thereof |
| CN115301719A (en) * | 2022-08-26 | 2022-11-08 | 南京大学 | Organic contaminated site remediation method based on biomagnetic resin |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105710122A (en) * | 2016-04-27 | 2016-06-29 | 上海大颂生物技术有限公司 | In-situ mechanical remediation method of urban landscaping planting soil |
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| CN110846041A (en) * | 2019-11-15 | 2020-02-28 | 黑龙江惊哲森林食品集团有限公司 | Soybean water lactobacillus soil remediation agent and preparation method thereof |
| CN112470587A (en) * | 2020-10-30 | 2021-03-12 | 中国农业科学院草原研究所 | Method for quickly repairing surface soil of degraded grassland |
| CN112470587B (en) * | 2020-10-30 | 2021-10-22 | 中国农业科学院草原研究所 | Method for quickly repairing surface soil of degraded grassland |
| CN114467405A (en) * | 2022-02-10 | 2022-05-13 | 自然资源部天津海水淡化与综合利用研究所 | Method for restoring saline-alkali soil by culturing indigenous microorganisms and application thereof |
| CN115301719A (en) * | 2022-08-26 | 2022-11-08 | 南京大学 | Organic contaminated site remediation method based on biomagnetic resin |
| CN115301719B (en) * | 2022-08-26 | 2023-10-20 | 南京大学 | Organic pollution site restoration method based on biological magnetic resin |
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Application publication date: 20140423 |