CN114457180B - 猕猴桃品种分子鉴定的mnp核心引物组合及其应用 - Google Patents

Abstract

本发明属于分子生物学技术领域，具体公开了猕猴桃品种分子鉴定的MNP核心引物组合及其应用，所述的引物组合如表1所示。本发明提供了347个MNP核心引物构成的引物组合可兼容二代测序平台，具备高效、正确、高重现性等特点，满足猕猴桃标准指纹数据构建的要求；对猕猴桃待测样本和对照品种鉴定，能够准确鉴定所述待测猕猴桃样本的真实性；这套MNP位点具备高多态性和可重复性，能区分不同猕猴桃类群，具有广泛的应用范围。

Description

猕猴桃品种分子鉴定的MNP核心引物组合及其应用

技术领域

本发明属于分子生物学技术领域，具体涉及猕猴桃DNA品种分子鉴定的MNP核心引物组合及其应用。

技术背景

猕猴桃(Actinidia chinensis)为猕猴桃属经济作物，果实营养丰富，在全球各地广受欢迎。中国是猕猴桃属植物的原产地和猕猴桃遗传资源的原始起源中心。

随着猕猴桃新品种的日益推出，新品种的鉴定是亟待解决的一大难题。仅通过树势、叶片形态、花期、花形态、果实外观等传统手段对猕猴桃品种进行鉴定，难免受到环境和其他人为因素的影响。对猕猴桃品种的不确定性，限制了产业的健康持续发展，且苗木品种不纯甚至错误将造成果农经济效益严重受损。

分子标记技术通过检测生物个体在基因组上产生的变异，从而对不同个体进行区分，是继形态标记和生化标记之后，发展的一种较为理想的遗传标记。DNA指纹鉴定技术由于不受环境和人为因素影响，且具有快速和准确的特性，已成为目前猕猴桃鉴定的重要手段。早期传统的DNA指纹鉴定技术有RFLP、RAPD、ISSR、SRAP标记法技术，这些标记具有分型不稳定、基因型过多无法分辨和非共显性等缺陷，基本上没有被纳入品种鉴定中。经过近几十年的发展，最终只有SSR和SNP标记鉴定技术被保留并应用品种鉴定中。SSR标记法的有主要优点是多态性高和共显性，可在普通实验室进行电泳检测。目前大部分的植物指纹图谱和品种鉴定标准都是基于SSR开发，但涉及到大规模样本检测时，无法做到同时检测成百上千的已知品种，并且涉及到近似品种筛选、品种权认定和实质派生品种判断时，不具有现实的可行性，因此SNP标记法在近年来被逐步应用于植物品种鉴定中。SNP标记法主要是获得单个核苷酸酸碱基序列，因此不需要对照平行实验。然而SNP也有一些自身固有的缺陷，如SNP标记是二态性标记，单个标记区分能力有限，所以需要检测大量的SNP位点才能实现精确分型，因而设计到高通量芯片技术。基因芯片技术的生产工艺较为复杂，生产成本较高，不太适合普通实验和小作物等中使用，推广力度有限。

MNP多态性是指在基因组水平上有多个核苷酸引起的多态性。MNP标记法技术主要是基于高通量测序技术开发的检测特定基因组上一段区域内的由多个核苷酸多肽性的标记法技术。与SSR标记和SNP标记法相比，MNP标记法在构建植物指纹图谱和品种鉴定方面具有以下优势：(1)等位基因丰富，单个MNP位点上有2ⁿ种等位基因，高于SSR和SNP；(2)MNP获得的是碱基序列，不需要平行对照实验；(3)MNP标记的等位基因型丰富，品种区分能力强，只需要少量的MNP标记就能实现品种鉴定，减少了检测错误率；(4)可定量检测，基于MNP位点利用二代高通量测序重复抽样可对特定等位基因进行定量分析；(5)效率高，利用二代高通量测序仪可实现成百上千份样本的数百个MNP位点分型；(6)可进行实质性派生品种检测。

鉴于以上优点和特性，MNP标记法已广泛应用于水稻、玉米、番茄、辣椒等作物中。目前在猕猴桃中尚未有MNP标记指纹图谱对品种鉴定的标准。

MNP标记主要基于参考基因组序列开发，根据已报道的猕猴桃重测数据和测得的差异性样本材料的重测序数据可以挖掘大规模的多态性MNP位点，再根据多态性MNP位点遗传多样性差异可筛选多态性最大、覆盖程度的最广的一套MNP位点，通过MNP位点两侧的保守序列可以设计MNP检测引物。根据研究目的筛选300至1000个左右即可完成大规模植物DNA指纹图图谱构建、真实性鉴定、遗传多样性分析、分子育种等工作。因此，本发明基于已公布的猕猴桃重测数据及自行布置的猕猴桃测序数据，结合高通量测序技术开发了一套用于检测猕猴桃多态性MNP标记位点的引物，并用于构建猕猴桃DNA指纹图谱，相关MNP标记法引物适用于猕猴桃真实性鉴定、遗传多样性分析、分子育种等领域。本发明在猕猴桃领域属于首创，并未见相关文献报道。

发明内容

本发明的目的是提供猕猴桃DNA品种分子鉴定的MNP核心引物组合，所述的引物组合为位点编号MHT0001～MHT0347对应的引物。

本发明的另一个目的在于提供了猕猴桃DNA品种分子鉴定的MNP核心引物组合的应用。

为了达到上述目的，本发明采取以下技术措施：

猕猴桃DNA品种分子鉴定的MNP核心引物组合，所述的引物组合为位点编号MHT0001～MHT0347对应的347对引物，具体的引物序列如具体实施方式中表1所示。

猕猴桃DNA品种分子鉴定的MNP核心引物组合的应用，包括用于构建猕猴桃DNA指纹数据库、检测猕猴桃品种真实性、猕猴桃种质资源遗传多样性分析或猕猴桃分子育种；

以上所述的应用中，当用于构建猕猴桃DNA指纹数据库时，是利用本发明的引物组合对待测猕猴桃品种的基因组DNA进行混合PCR扩增，347对MNP引物构成的组合加上二代测序接头引物和标签序列后对回收产物进行第二轮混合PCR扩增，对第二轮扩增产物回收和纯化后进行上机测序，测序结果比对回所述的猕猴桃参考基因组上，获取上述猕猴桃品种在所述347个MNP位点上的基因组数据，即构成猕猴桃的DNA指纹数据库。

以上所述的应用中，当用于检测猕猴桃品种真实性时，利用所述的MNP引物对待测猕猴桃样本和对照猕猴桃样本的基因组DNA进行扩增和测序，从而获取待测猕猴桃样本和对照猕猴桃样本各自在347个MNP位点的基因型数据，若所述待测猕猴桃品种与所述对照猕猴桃品种在所述347个MNP位点上的基因型数据中的差异位点百分比大于等于5％，则说明两个猕猴桃样本不属于同一品种；若所述待测猕猴桃品种与所述对照猕猴桃品种在所述347个MNP位点上的基因型数据中的差异位点百分比小于5％，则为同一品种或者候选同一品种。

以上所述的应用中，猕猴桃种质资源遗传多样性分析时，是分别提取待测种质资源群猕猴桃品种的基因组DNA；利用所述的MNP引物对所述猕猴桃品种的基因组DNA分别进行扩增和MNP位点查询，从而获得所有所述猕猴桃品种在所述的347个MNP位点上的基因型数据；建立聚类树状图。

与现有技术相比，本发明具有以下优点：

实验证明，本发明提供的这个347个MNP位点检测引物，可兼容二代测序平台，具备高效、正确、高重现性等特点，满足猕猴桃标准指纹数据构建的要求；对猕猴桃待测样本和对照品种鉴定，能够准确鉴定所述待测猕猴桃样本的真实性；对猕猴桃材料分析显示，这个MNP位点具备高多态性和可重复性，能区分不同猕猴桃类群。因此本发明所提供的347个MNP引物可应用于猕猴桃品种DNA指纹图谱构建、品种真实性鉴定等相关研究中。本发明提供的347个MNP标记引物组合在猕猴桃中的应用将加快我们猕猴桃品种标准MNP指纹数据库的构建进程，推动MNP标记数据在品种真实性及实质派生性鉴定中的应用，为我国猕猴桃品种权管理、品种维权等方面提供有力的科技支撑，确保猕猴桃生产安全；同时，也为猕猴桃分子育种及种子资源分析提供了技术支撑，加快了猕猴桃新品种选育周期。

附图说明

图1为347个MNP标记在染色体上分布图。

图2为91份猕猴桃自交系样品被划分到不同的群内的聚类分析图。

具体实施方式

下述实施例中所使用的实验方法如无特殊说明，均为常规方法；所用的材料、试剂等，若无特殊说明，均可通过商业途径获得。

实施例1：

猕猴桃DNA品种分子鉴定的MNP核心标记引物组合的筛选和验证

(一)猕猴桃MNP引物标记组合筛选

以公开发布的猕猴桃基因组为参考(http://eggplant.kazusa.or.jp/)，利用本团队开展的76个猕猴桃基因组重测序数据进行比较分析，筛选到347个MNP标记位点(图1)，设计引物(引物详细信息见表1)。

(二)猕猴桃MNP引物标记组合正确性分析

为了检验猕猴桃MNP标记法的准确性，从国家猕猴桃资源圃采集8个样品，进行了重现性实验，每份品种的同一份DNA分别构建了2次文库，2个文库不同时间测序两次。不同批次文库第一次和第二次测序比较，比较差异位点数目都为0，统计结果见表2：

猕猴桃MNP标记法准确性分析结果。同一样品的不同文库间检出相同的MNP标记基因型是可重现的，按照重现性的计算公式r＝n/N，n:可重复的基因型对数，N：比较的基因型总对数。依据2次实验间可重现的基因型认为是准确的原则，准确率＝1-(1-r)/2＝0.5+0.5r。本项目重现性试验中每个样品不同文库间MNP标记基因型差异对数为0，r＝1,准确性为100％。

表2猕猴桃MNP标记法准确性分析结果

因此，获得了猕猴桃DNA品种分子鉴定的MNP核心标记引物组合，引物组合如表1所示。

实施例2：

猕猴桃DNA品种分子鉴定的MNP核心标记引物组合构建猕猴桃标准DNA指纹

1、DNA提取与质量评估

利用常规CTAB法提取用于构建猕猴桃指纹数据库的所有猕猴桃品种的基因组DNA，RNA酶处理后用琼脂糖凝胶和紫外分光光度计检测DNA的质量。若DNA条带单一，无明显降解，紫外分光光度计检测A260/280介于1.8-2.0，且A260/230介于1.8-2.0，270nm出无明显光吸收，DNA浓度大于50ng/ul，则说明基因组DNA达到相关的质量要求，可进行后续实验。

2、MNP标记检测

利用合成的347个猕猴桃MNP标记引物对中科院武汉植物园提供的91份猕猴桃品种进行了多重扩增、二代高通量测序与数据分析。本次结果实现了一次实验达到91×347＝31577个标记的检测，显示了MNP-seq的高效性。基于91个猕猴桃品种的MNP数据进行分析，每个品种平均可以检出82％，检出率高。

3、基因型指纹数据

将比对后的基因型位点数据导入Excel 2017软件，记录每个样品347个位点的基因型，作为最终的MNP指纹图谱数据。

实施例3：

猕猴桃DNA品种分子鉴定的MNP标记引物组合检测猕猴桃品种真实性

1、猕猴桃MNP引物标记品种区分度及猕猴桃品种真实性检测结果判定标准的确定

对实施例2中的91份猕猴桃样品进行两两比较，分析其MNP标记位点的差异，共得到4095对比较结果，任意品种间MNP标记平均差异数量89.22个，4095对样品的遗传距离的差异位于0.30％-82.26％之间，平均差异达47.85％，两两比较中没有出现差异为0。利用所开发的标记检测方法，100％的猕猴桃品种可以被区分(图2)，说明开发的猕猴桃MNP标记多态性高、品种区分力强。因此347个位点完全可以应用到猕猴桃品种真实性鉴定中。此外，差异位点百分比低于5％的成对比较样品较少，占据0.098％，说明91份猕猴桃具有同一的基因型来源的较少，适合作为猕猴桃品种检测的标准数据库。

根据以上猕猴桃MNP检测结果及其他作物参照，将差异百分比5％作为猕猴桃品种真实性判定的标准。

所以，利用本发明进行猕猴桃品种真实性检查具体判定标准如下：

获得待测样品与对照样品(即标准样品)347个MNP位点的基因数据，并进行品种间的数据比较，获得差异位点百分比。差异位点百分比p＝(差异基因型位点数n/比较基因型位点数目N)×100％(其中N最大值为347)。

(1)待测样品与对照样品差异位点百分比p≥5％，结论为检测到明显差异；

(2)待测样品与对照样品差异位点百分比p<5％，结论为未检测到明显差异

2、鉴定待测猕猴桃样品是否为某已知对照猕猴桃品种

待测猕猴桃样品1(代号A)和对照猕猴桃品种(代号B)；待测猕猴桃样品2(代号C)和对照猕猴桃品种(代号D)；提取上述两组检测样品的基因组DNA，进行DNA质量检测、MNP标记鉴定，获得基因型指纹数据，具体方法见实施例2。

将样品A和样本B的所获取的MNP位点基因型数据进行比对，并将样品C和样本D的所获取的MNP位点基因型数据进行比对。

结果如下：

(1)样本A(待测猕猴桃样本1)和样本B(已知猕猴桃品种东红)比较位点数为338，差异位点数为2，差异位点百分比为0.6％；

(2)样本C(待测猕猴桃样本2)和样本D(已知猕猴桃品种翠玉)比较位点数为321，差异位点数为54，差异位点百分比为16.8％。

根据5％的判定标准，鉴定的待测样本A与猕猴桃品种B为同一品种或者近似品种；而待测样本C与猕猴桃品种D不是同一品种。

实施例4：

利用实施例2中的指纹信息对猕猴桃资源进行类群划分

本发明提供的347个MNP引物可用于进行猕猴桃指纹图谱构建，考虑到区分猕猴桃各杂种优势群的基因型位点，因此可以用于猕猴桃种质资源分析，如群类划分。

将实施例3中的91个猕猴桃的347个位点基因型指纹数据利用Power-Markerv3.25软件，选用欧式遗传距离算法UPGMA进行聚类分析，并用MEGA7软件绘制聚类树状图，91份猕猴桃自交系样品被划分到不同的群内。结果如图2所示。

表1 347个MNP标记扩增引物序列信息表

/>

/>

/>

/>

/>

/>

/>

/>

序列表

<110> 中国科学院武汉植物园

<120> 猕猴桃品种分子鉴定的MNP核心引物组合及其应用

<160> 694

<170> SIPOSequenceListing 1.0

<210> 1

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1

gtacttcaac taacatgtcg tacccgt 27

<210> 2

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 2

gtcttaacaa acctaaccat caaaggttaa ta 32

<210> 3

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 3

agctataaag aagtagttga tgtatggagg aa 32

<210> 4

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 4

ctttacaact caaactcaag taaaggaaat gg 32

<210> 5

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 5

cgtgacatca ctagctcatt gagtaaatag aa 32

<210> 6

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 6

tctgtccctt acaaaattaa gaacaaaaat gc 32

<210> 7

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 7

gtgagaaaaa taaatatcct cctaaaagcc ct 32

<210> 8

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 8

gatttttagg gacaggttta tggaactgat tc 32

<210> 9

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 9

ttctgactgt atctaacggt tattgtcttc ta 32

<210> 10

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 10

caaatttggt tattaccata gcatcagtcc ta 32

<210> 11

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 11

gcatatcaat ccaattagtc attagaaatc ca 32

<210> 12

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 12

ttcttgggtt cgattcgtat ttgcac 26

<210> 13

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 13

aatctgaact attcatttta catcctggga at 32

<210> 14

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 14

aattcagtcc taccagcata agataatgaa gc 32

<210> 15

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 15

tactagttca tcttaatagg ccctcgagta tt 32

<210> 16

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 16

aagatcataa tggaaaaatt ccaaattgcc ag 32

<210> 17

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 17

ttcgagtagc ttagtttatt tatcactgct at 32

<210> 18

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 18

taggtatgct ctcttctaaa gatttcaagt tc 32

<210> 19

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 19

ataattcttt tgagctttat gtttgtgacg ac 32

<210> 20

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 20

tattgaataa ccgagggcaa tttacttacg 30

<210> 21

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 21

ctaaaaggtt tcgaacttga gatcttggaa 30

<210> 22

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 22

atgagatttg gaggtgtagt taagaatgga c 31

<210> 23

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 23

gaaattcaga atcctctctc tctctctctc tc 32

<210> 24

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 24

ctgtcgaatt ctgtcgtcac tctcag 26

<210> 25

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 25

aaaatgatca cacatcatat agttctctag gc 32

<210> 26

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 26

cttcctacat gagacttcct ggtgac 26

<210> 27

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 27

aatgaatttg atcctttaaa atggaggatt gg 32

<210> 28

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 28

aatacatcca acaatagttt aattagtggg gc 32

<210> 29

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 29

ataaacatgt aatttctccg ttattggtcg at 32

<210> 30

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 30

ggtgaacaga gaattatgag tcctaagaaa ag 32

<210> 31

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 31

ttttatttgt tattcgcaca agcgtttttc ta 32

<210> 32

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 32

atttataacc agtatcagat cttaatcggg gg 32

<210> 33

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 33

tttcgatgaa aattagtgag tgtgagttac tg 32

<210> 34

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 34

cttctcagta aggatgcaaa aggaaataca at 32

<210> 35

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 35

tgaatctgga taaaatcttg actctcatta gc 32

<210> 36

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 36

aataatcttg agactttatc tctaccacga ct 32

<210> 37

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 37

tggtgctcat caaagtctca tcaaaataaa ta 32

<210> 38

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 38

ctttatccat gttgattcca ttttcttttc gg 32

<210> 39

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 39

ctttttgcca aatttttgat acgagtttca tc 32

<210> 40

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 40

actagtttta ctattccagc actacatctc tc 32

<210> 41

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 41

gacttgtcta ctttgaattg tatttatccg gt 32

<210> 42

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 42

gcttaattaa ttcggttttg acacatcatt ct 32

<210> 43

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 43

ctggatatag atcaaaccac caaaactatg ag 32

<210> 44

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 44

atttggataa ggttcggaaa tactgaaaaa tg 32

<210> 45

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 45

ttagaactat tcatgttata aggctcaacg aa 32

<210> 46

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 46

caaaagttat cccctctaaa tttatccctc tt 32

<210> 47

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 47

ttaattttga ttcattggtt tgaccaactt gt 32

<210> 48

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 48

gctcactttg tatttttatt ttccttcctc ac 32

<210> 49

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 49

taaatattgt gtctgtgtgt atgcgtaatc tg 32

<210> 50

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 50

catagatgag aagttattca atgctctcag aa 32

<210> 51

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 51

gactattttt cttgccctaa ttgtgtcttg tg 32

<210> 52

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 52

agttttgttg ttggttatcc gctctctc 28

<210> 53

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 53

tcttaggatt tacgttttct tttgagagac ag 32

<210> 54

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 54

aacaaaaagc ataaatttag aacaacttca ca 32

<210> 55

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 55

gccacatgac tcttgactta aactataatg a 31

<210> 56

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 56

ataatgacaa ctagtagtat caagaaggct ca 32

<210> 57

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 57

tttgtaaggg atttggatag agggactgta tt 32

<210> 58

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 58

aaatccgttg aagatgttga cgagaaatat aa 32

<210> 59

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 59

acccctagtt taaatccata tgtaaaacat cg 32

<210> 60

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 60

ttatgaagca ggttattgta tttgttgcat tc 32

<210> 61

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 61

aaaaagaaat gcgaagaaag aaaatgagac at 32

<210> 62

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 62

atattagatg gtattagtgt tcccttcaca ca 32

<210> 63

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 63

ctcggcagtt gctatgtcaa actatg 26

<210> 64

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 64

ttgtcttaag atttgtttcg aataggacag ag 32

<210> 65

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 65

tacattgaac ttgtttgtga ttgactactt ct 32

<210> 66

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 66

taatacgtag atttaccatt actgctcatt gc 32

<210> 67

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 67

ctactcttgc tatagtcctc tacatttgct tc 32

<210> 68

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 68

ggttgcagaa cacaaacttc acattgat 28

<210> 69

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 69

tgaacttgct tctgttgata aatgctaaaa at 32

<210> 70

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 70

gaccttttgg tataaatagt gggatggttt ta 32

<210> 71

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 71

ctccctagct cattgaatcc taatcaaact c 31

<210> 72

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 72

gtctaattca agaggattgg ctatggagtg 30

<210> 73

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 73

ataatataaa ctgtggggtg taggtttagg aa 32

<210> 74

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 74

tcaacattta tttctctaaa aggtacgcga at 32

<210> 75

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 75

aaactgtttt tacaacaccc tttggacatt ac 32

<210> 76

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 76

aattcaactt taggagcaga gattactaga cc 32

<210> 77

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 77

tagtgaagcc aaattttcaa agcaaaaact ac 32

<210> 78

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 78

catttgaatt ggatgagaaa caagaaaaga gt 32

<210> 79

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 79

agaactgatc aaatctttta tacctttgtc gc 32

<210> 80

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 80

aataaggtat tctctcatgg tgttaagcat tc 32

<210> 81

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 81

aatgtggaac ttttcaactt tactcacatt tc 32

<210> 82

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 82

taattcgtca tagctcgagt ttcatttgtt at 32

<210> 83

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 83

tgtgggtaat taaaggtggt aaattcagtt ag 32

<210> 84

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 84

ctatgttgga gagaatacaa gttctcaagt tt 32

<210> 85

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 85

tccattgctc cttcaaaaga tctctattta tc 32

<210> 86

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 86

caatattgtt tctaaaatct gcaatacgcc at 32

<210> 87

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 87

gtgttactgg aatttacaat atccttctcc at 32

<210> 88

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 88

ggtacatctc tttttgaagt ggttagtctt tg 32

<210> 89

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 89

atcattagtc taaagcatca taaaacctgt ct 32

<210> 90

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 90

atgaccaaat atctatcaat gtcaactgat gc 32

<210> 91

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 91

tctctacagt taatccaaac tgttcatatt gc 32

<210> 92

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 92

agcaaactac aatatccttt tatctttttg gc 32

<210> 93

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 93

atttcagtca caaatgagtt caatactggt c 31

<210> 94

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 94

actttcacaa gtttatttgc ttcataatta gc 32

<210> 95

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 95

acagttactg gatataattc tttccttttc gc 32

<210> 96

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 96

atcattatca ctcacatcga caaccatttt at 32

<210> 97

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 97

catgtataaa tgggttgggt cgtgacatag 30

<210> 98

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 98

taagatttgg acaatataaa tttgcacaca gg 32

<210> 99

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 99

caatcaaatc tctgaattca tcaatcttct gc 32

<210> 100

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 100

tggagtagaa aacctataac tactaaaacc ga 32

<210> 101

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 101

atcctttcat atccatataa cagcactatc ca 32

<210> 102

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 102

agagatgaca aatgatggtt taaactgaag aa 32

<210> 103

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 103

caaaaaggaa tgtgagacaa taataccctc at 32

<210> 104

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 104

gggttgtcaa atcaacataa gaatgcaaat ta 32

<210> 105

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 105

tatatcttag gtcatgtgtc aatgtgacct ct 32

<210> 106

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 106

ttttcaactt ggattagtta cactttgtac cc 32

<210> 107

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 107

aatattcttc atcccaatcc caaattgtgt tc 32

<210> 108

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 108

atagagaaga gggatcttga atagacaaag ag 32

<210> 109

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 109

tattgacata tacagaatca cagtggtagg aa 32

<210> 110

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 110

aatcttcaga aggcatcaat atttaaccaa gt 32

<210> 111

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 111

caaagaacac gtatcttctt agcgagtc 28

<210> 112

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 112

gttatcttcc tagttttcca gcagtaaaga ac 32

<210> 113

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 113

agtttgatgg atttatgacc aattatccca tt 32

<210> 114

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 114

cttcagtaac taaaacccta gctcttctct ac 32

<210> 115

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 115

aggaaaaata tagctcaaag ttctcaaagt ga 32

<210> 116

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 116

tatgtcctca accctcttga tcatatctta tt 32

<210> 117

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 117

gagaaaatgg agtttccgag aaaatgtaaa ag 32

<210> 118

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 118

accttgaaag tgtacttctc ctaaatacat tg 32

<210> 119

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 119

ataaaaattt tgacaaagct gaaggaaaag cc 32

<210> 120

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 120

tctgatatac acaacgcttt tagctgtgtt c 31

<210> 121

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 121

atcagaaata gagtctgaag aaactatcgg ag 32

<210> 122

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 122

gattagggtt taaattgctg aactttgtga ag 32

<210> 123

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 123

gaatcgactc aactgttcca tttaaagaca tt 32

<210> 124

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 124

aacctaagaa gaatttagta tttgaaagcc ct 32

<210> 125

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 125

acaggaaata aatttttgct tcccctaata aa 32

<210> 126

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 126

taggagttgc aaaattaagc catttcagag 30

<210> 127

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 127

taaaatcgag gaactgattg tatttcatcc tt 32

<210> 128

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 128

tagatctatt taaatgtcgg tcttggtttg ac 32

<210> 129

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 129

gttcaaaaat ggagaaatat agatcatccg aa 32

<210> 130

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 130

ttccttcgat ctattgacaa attaagtaac gg 32

<210> 131

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 131

atttgaacta aatatcttgg ggatgtgctt c 31

<210> 132

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 132

gactatccca tctctaaagc aatatccatt tc 32

<210> 133

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 133

taattacaat ctcactcatt gagctattgc at 32

<210> 134

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 134

aatgaaagtt gttgtaatag aaatttgagg cg 32

<210> 135

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 135

agttttgtta ttgattgtac tttattggcg tt 32

<210> 136

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 136

tccgtgtatc aaaaagctct acaaaatcta aa 32

<210> 137

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 137

tcatggcttt acctataaaa aggttagttt gt 32

<210> 138

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 138

agcaatatca gtctcattaa gaaagattcg tc 32

<210> 139

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 139

aaaaattact cgaggttttg gcactaattc ta 32

<210> 140

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 140

tcaatatggc caaaaataca attgaatagg ct 32

<210> 141

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 141

tgtaagttgt atgaaaatgg aaaaatgact gc 32

<210> 142

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 142

tacatttatt tacctttgcg actagaaagt ca 32

<210> 143

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 143

gtaaagtact atggcagaca ctctacactt ta 32

<210> 144

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 144

tgctgtccaa gttttggatt cttttattgt at 32

<210> 145

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 145

aaatattgaa gcgttaaaga ctacctcatt cc 32

<210> 146

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 146

tattctaaat cattccactg acattctgtg tc 32

<210> 147

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 147

ttttgtcatt ccccattttt attggtttat cg 32

<210> 148

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 148

atcatacctt gaatccaaac acttatttac cc 32

<210> 149

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 149

ttacttctct cactagtctc tttcactctc tt 32

<210> 150

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 150

gagagtttcc ggtagagaac ggc 23

<210> 151

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 151

cacaactttg atccattcca gctcag 26

<210> 152

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 152

caaggatttg ttgttgttga actatttacc tc 32

<210> 153

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 153

gtcaaaaagg aatacaaatt gaacctaagc ac 32

<210> 154

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 154

gttcttgaat cgtaaatctg aagcttgatt tg 32

<210> 155

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 155

cagaaattgt caactgtttt taggatcttg tt 32

<210> 156

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 156

tagtggcgtt ctaattttaa agtaattggt ct 32

<210> 157

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 157

gttattcgat gatatcaagt gttttacctc gg 32

<210> 158

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 158

atagtaccac aaatatagta gacctgatgg ag 32

<210> 159

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 159

caattactaa ggctgtattt ggatcaagga tt 32

<210> 160

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 160

atttgccaat aggcatcttt aggttatgtt tg 32

<210> 161

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 161

ttttaaaaca ttttatatgg tcaaggctcc ct 32

<210> 162

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 162

cagatctatg gaattgtttc tgaaattgtg tt 32

<210> 163

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 163

tacgttattg ttcatttgcg tttagcaact at 32

<210> 164

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 164

gaaatatcaa cgggaaagac tatgtgaggt ag 32

<210> 165

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 165

atgactgcat tcaaattcct acttttatct gt 32

<210> 166

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 166

atatgtgctt gcatttattc tcttttggtt tc 32

<210> 167

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 167

aatttggaat tttgttcgtt tgcttatttt gg 32

<210> 168

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 168

atttcccatg attttgataa atttcttcac cg 32

<210> 169

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 169

caagtagaat cacattgatt tgtggtttgt ag 32

<210> 170

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 170

actttcgaaa tgttgaaaag aacttgcatt at 32

<210> 171

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 171

agaagactta catttcgctt tatcaatggt ag 32

<210> 172

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 172

gtgccattta atctcctcct atcacattta ag 32

<210> 173

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 173

gaagcggacg tgtatgtgtt cg 22

<210> 174

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 174

caaagctctc tttacgattt cgccttc 27

<210> 175

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 175

ataaatcata cacgccaaat aaattccctg aa 32

<210> 176

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 176

ggtagacatg cggtagtaac aaaatataca at 32

<210> 177

<211> 21

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 177

cacaagctcc tccccttctg g 21

<210> 178

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 178

aacatcatcc atgcctttat gttcatcctc 30

<210> 179

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 179

ttcctctctt ccgatttcat ctgaaaatct ac 32

<210> 180

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 180

gagttgtgtg ggtttaaaaa tttggttcat ag 32

<210> 181

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 181

attcaagtcc aatattttct tgtctcttct cc 32

<210> 182

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 182

cagcttcttc gattcgatcc acac 24

<210> 183

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 183

ctgtagttag caaccgacct caatatcata c 31

<210> 184

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 184

tattgatcta atgcatcttg gctaatgagt tg 32

<210> 185

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 185

agtcagaaac ttcaattccc aggaaaaact at 32

<210> 186

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 186

caaatttggg tgaaattctt gatttggttt tg 32

<210> 187

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 187

actcagtggt taggactaat ttgacatatt ct 32

<210> 188

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 188

tcaatgtatg aactacacaa ggagaccaaa tc 32

<210> 189

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 189

tttatgtttt tgttaggtct gttgtttcaa gg 32

<210> 190

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 190

ttgatggtat gggatatgtg tttggtattt tt 32

<210> 191

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 191

agaatttagg atatagagat tcgatggaga cc 32

<210> 192

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 192

ctctcttctt taatatcaat agcaaacgca ac 32

<210> 193

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 193

ctccccttta tagggttaag attctacttc tt 32

<210> 194

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 194

aaatgttgtc gagtttcttt ttctattggt tc 32

<210> 195

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 195

ttcacctgaa gttgattatt tattgaccac at 32

<210> 196

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 196

aatctaaatt gttcatcttt cagatgtcgt tg 32

<210> 197

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 197

gtatctaatg gaaatttctt acattttgcg cc 32

<210> 198

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 198

aatgttgatg aatttgttat ttacggaaag gc 32

<210> 199

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 199

ttttaaaatc tgaatagttc actcagactg cc 32

<210> 200

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 200

atttgacaaa atccacacat tttcttccta tt 32

<210> 201

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 201

tcgaaatata attctagtat gacaaggagt gc 32

<210> 202

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 202

agggtttctt agaatctatc tcaaccttaa ct 32

<210> 203

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 203

gctactcgtg taacctggta aataacaaat aa 32

<210> 204

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 204

tgaactgatt caactcagaa attgatagta cc 32

<210> 205

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 205

gaagaattta gtcaaagcct tttcataacc ac 32

<210> 206

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 206

cttttcatac tcttgaaatc gagctgcttc 30

<210> 207

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 207

aattacgtca ttttgagaaa ataaattcgg ca 32

<210> 208

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 208

gttctttcca ttataggact atttattggg ct 32

<210> 209

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 209

aatcaaaatc aaactttagt aaatccgtgg ac 32

<210> 210

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 210

atacttgtct atttactcaa tccttgtgaa cg 32

<210> 211

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 211

cttttctatg ttttatgctt gggagctaat tg 32

<210> 212

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 212

gttccagtct aaaactccct aaattgtgta at 32

<210> 213

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 213

gaagaactta acgtgtgtac caagatctga at 32

<210> 214

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 214

gtagtctcac ctactctatt tcaatttcaa gc 32

<210> 215

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 215

acttcaatta ctcaaaatgg acaaaagtat cc 32

<210> 216

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 216

cctacctaac aaccaccact tatcactatc 30

<210> 217

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 217

tgtatttctc aacgcattca tattctctca at 32

<210> 218

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 218

caccaatgtt tccaaaaagg aggtttcttg 30

<210> 219

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 219

ccttagtcta ttcctataga tctaccccat ct 32

<210> 220

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 220

ctagcatatc agcataccaa agactagatt tc 32

<210> 221

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 221

ccaattctgg gaaattgggt cgaaaac 27

<210> 222

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 222

ctcttctttt acccacccaa atcacctc 28

<210> 223

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 223

gaataggata gctcacgatt cctatgtttg 30

<210> 224

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 224

ctcttccaag aagctaagag aacctctgta a 31

<210> 225

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 225

ttttaaacca accttaaacg actcactaca at 32

<210> 226

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 226

tatttaggaa aactgattaa tgtagccaac cc 32

<210> 227

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 227

ttctcactat ttctatcttg caggccatat ta 32

<210> 228

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 228

tatgttggag aattgtgtaa atatgtagcg tt 32

<210> 229

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 229

ctctctcaaa ttaatcctaa ctgcttccct ac 32

<210> 230

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 230

atatgtttac gatctctctc aatttctctg ga 32

<210> 231

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 231

aatattatga tgaaacctgt tgctgtttag gt 32

<210> 232

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 232

aataaataca gtcacacaat tagtaccttg gg 32

<210> 233

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 233

tgaacgagat caagaacgta atgaaaattt ag 32

<210> 234

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 234

aggttttctg tggaacctaa tcatgaaata aa 32

<210> 235

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 235

tttattccga aatccatttc atttaaccaa cc 32

<210> 236

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 236

ttggatttat cactcaagga aaaattttgt gg 32

<210> 237

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 237

gttgtctaag gaatgatgtg atagaatatg gg 32

<210> 238

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 238

agaaccatat atgagaatgg tattggaact ct 32

<210> 239

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 239

tttttgattg atgttgttgc taccttgtt 29

<210> 240

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 240

aagagggggt cgaataacaa ataagttaaa ta 32

<210> 241

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 241

acgctcaagt agaacaaatt agaacataat ga 32

<210> 242

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 242

tagattcata atttcagcta tatgggaccg tg 32

<210> 243

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 243

ccttctgaac ctattttaca ctttctcgaa ta 32

<210> 244

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 244

gggttattca ttgttaaaag aatggggaat tg 32

<210> 245

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 245

taggagttga gggtttgcat agttcttaat 30

<210> 246

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 246

aattatcagt ttaaccattc ttacagcagc c 31

<210> 247

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 247

ttttcagtag aatacttgga tgaagaactt gg 32

<210> 248

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 248

acagaagtta aactcaacca taagctgtat tt 32

<210> 249

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 249

catgaaaatc tcctccaagt cagcaatac 29

<210> 250

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 250

tttttcccct gtttcttcgt cttcactc 28

<210> 251

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 251

gctaactatt tcctaatgtt atagctgctc aa 32

<210> 252

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 252

caaaagagat atatgtttgg aaagaactcc cc 32

<210> 253

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 253

atttagtaga aatatacccc caaaagtgtg ac 32

<210> 254

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 254

gacttaatcc agtgagtttt cttactatgg tc 32

<210> 255

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 255

tattcgcaaa cagctaaaat attactgcct at 32

<210> 256

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 256

tttgtctatt aagctacgtc cgagtgtttt ta 32

<210> 257

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 257

actctgatcc catcaaattg aataatatcg tc 32

<210> 258

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 258

tactagaaag ctatgggtat caacatctat gg 32

<210> 259

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 259

cagttatgaa tgaatggcat gaaatgaaat ga 32

<210> 260

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 260

aactgttaac tcttgagagt attagtgatc ca 32

<210> 261

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 261

gttttcttac taattgatgc taagtgggag at 32

<210> 262

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 262

tgaacacaca atattaaaag gattgaacat gc 32

<210> 263

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 263

cacacaaatc ggagagtatc tattacatga ag 32

<210> 264

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 264

tacatggtat gctgttatca ataattcgtc aa 32

<210> 265

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 265

cttatatagc agtttgaccc gtatcttgac 30

<210> 266

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 266

cttgcacaac gagttttcct ctaatagc 28

<210> 267

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 267

tccttcacat aaaatcattc tttgctatcc tt 32

<210> 268

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 268

aggttaaaga aaaactccta acaatcacag aa 32

<210> 269

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 269

gggacatatc atacaaagat agaatcttgc ct 32

<210> 270

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 270

tttgataacg actgctttga gctccg 26

<210> 271

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 271

atcatctgat ccaattcttt gttctaatcc tg 32

<210> 272

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 272

gatgttttga ttttagatgt tgtgcgtttg ta 32

<210> 273

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 273

agtgaatata aaaccctagc tctcctacg 29

<210> 274

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 274

aatataaatc tcacaaaccc tctccttctc tc 32

<210> 275

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 275

cttctaatga ttttcgcttt actcgatctc ac 32

<210> 276

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 276

gaggaacaat aatcaatcat gatcaacctc at 32

<210> 277

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 277

aataaaggct gaatttatat agcacagctc aa 32

<210> 278

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 278

gaagcttctt ttgacagtcc cattactc 28

<210> 279

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 279

aaaatcaatt atagacattc gccaagcctt tt 32

<210> 280

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 280

ctcaatggtt atatcatgat tgtctgctcc tc 32

<210> 281

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 281

aggattattg taatgcaggg tagtagttag aa 32

<210> 282

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 282

tatccccttc tgcttgtctt aattattgtc ta 32

<210> 283

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 283

cttgatttct tctctccttt ctccccttc 29

<210> 284

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 284

aataaaatta ttcctcccct gttctttttc cc 32

<210> 285

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 285

acatgttggg ttaattagtg atatgtagca aa 32

<210> 286

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 286

acaatatacc ggatgcattt aaatagcgaa ta 32

<210> 287

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 287

gaatagatga aaggaaaaag ggaagagtta gg 32

<210> 288

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 288

tcaactctgt aacccttcat actcatgttt ta 32

<210> 289

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 289

acgatatatg tatttctctc ggatactttg gt 32

<210> 290

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 290

atttctttca ttttctcatc tagccatcta gc 32

<210> 291

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 291

cttgtaactt caacctcagt tagattatcc ac 32

<210> 292

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 292

cttttagatc catcatccac caatttacag g 31

<210> 293

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 293

gcttctcttc ttctgaataa aattgggttt tc 32

<210> 294

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 294

gttcatgtgg tagtgcatag aatctcatca tc 32

<210> 295

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 295

caaagacatc tctcatgttc ttcttaatcc at 32

<210> 296

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 296

gatggactac tgttccacct taataaaaga c 31

<210> 297

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 297

aatttaatat gtgtgttttt gtgtgcgata cg 32

<210> 298

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 298

tataacgaat taaccaaaat gaagcagaac tc 32

<210> 299

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 299

ccgtcgataa tattctttta agcgtttttg aa 32

<210> 300

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 300

atagaggatt agagggatta gcaaatgagt ag 32

<210> 301

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 301

gacattgtac ataaactttg aatttgcacc at 32

<210> 302

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 302

ttgtccaata gcatggaata agttctctta aa 32

<210> 303

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 303

aaaaattatg atcgagtgaa ctcaccactg 30

<210> 304

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 304

ctatgatctc cgaactaatc tctcatctgt at 32

<210> 305

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 305

ccaaatatct tatgtgaacc tttcttagtg gt 32

<210> 306

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 306

catagaacag gggatatata atacttggag gg 32

<210> 307

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 307

taccattttc cttaacaaaa tcagaatgtt gc 32

<210> 308

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 308

ggtaattttt caactagagt gtaggaaagg tt 32

<210> 309

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 309

tactagtttt agtttttcta gttctacccc gt 32

<210> 310

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 310

ccttgaatta aaagatactt tcctctcctc tg 32

<210> 311

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 311

cttccttcca tacaaaattc tcatcaaaat gg 32

<210> 312

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 312

cagatttagt tgtggattga aataaactgt ca 32

<210> 313

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 313

agataagagg tccaattgaa gtctatcacc ta 32

<210> 314

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 314

taaacctatt tcgctataaa gcaagcaagt aa 32

<210> 315

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 315

ttttctttct caaaggtaca caaaaatagc tg 32

<210> 316

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 316

tgtggggttc ttgattaaga gaataaaaga aa 32

<210> 317

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 317

agtcaactag gtcagagttg taatctttaa gc 32

<210> 318

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 318

atatttgtga ttgttggatc ttgtgtttta gc 32

<210> 319

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 319

atcatataac tagctgaaaa ggacacaaat cg 32

<210> 320

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 320

tacttgttga gtaaaagaaa gaatccaaca ca 32

<210> 321

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 321

aaaagagact gtgaatcctc ttattatcag gc 32

<210> 322

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 322

ttcttcttct ttttcttctc ctcctcattt tt 32

<210> 323

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 323

taaaatacga gttcggatac aggaacactc ta 32

<210> 324

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 324

tatatatatt attttgaaac cacgccgcta cg 32

<210> 325

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 325

attttcttta atctgtgact gataggggtt tg 32

<210> 326

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 326

aacaaaaact atgagatgaa taccgatcaa gg 32

<210> 327

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 327

ctaatgtcaa gaactcttct attgctctct tc 32

<210> 328

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 328

gattttgtga gaataatagg aaatcgagcg ta 32

<210> 329

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 329

ctcaagaaga ggaaacagaa gaattgctaa g 31

<210> 330

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 330

atttcatcga aattgtcaca cttgaaaatg g 31

<210> 331

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 331

tagtaatcca aacagatgat agaaggacaa ga 32

<210> 332

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 332

ccttaaccta ccaaatagaa ctttaacacg at 32

<210> 333

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 333

caaaattgtt attaattgct tcaagccaaa cc 32

<210> 334

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 334

ccctctatat caataaagcc cattaacatc ag 32

<210> 335

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 335

attataaata cgtagtagaa tttggcagtg gc 32

<210> 336

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 336

tgtttcatcc tttctattct catttctcct tt 32

<210> 337

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 337

cataacatca taagcactta ttctgcaaca tt 32

<210> 338

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 338

ttcagttcat tatcatgtga aattgttccc ta 32

<210> 339

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 339

accaatagaa ttatagaacc ccgctataga aa 32

<210> 340

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 340

tccgcattag gctagattag gtatattaaa gt 32

<210> 341

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 341

cgcaactttt cttggacata tcatcagtat aa 32

<210> 342

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 342

tcttcaatat ttgaaaactc ttttctcgct tc 32

<210> 343

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 343

actttgtctt tttgaactct attctctact ca 32

<210> 344

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 344

atggaactat catgttatac ccctcatatt gt 32

<210> 345

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 345

tatagaagtg caatattctc ctaatcgtag gg 32

<210> 346

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 346

aaaagaatca tctgatcatg gggaatatcc tg 32

<210> 347

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 347

ggaccttgtc atgttaaaaa caaaatccta at 32

<210> 348

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 348

ttgatatata atttctctgt tgctctttgc ct 32

<210> 349

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 349

aggtttgata agcaataatt gtagaaggaa gg 32

<210> 350

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 350

ttgtctgatt agtttgggag ctcgatttaa g 31

<210> 351

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 351

taatctggaa tctcactgaa acctctatgt aa 32

<210> 352

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 352

actcgtattt attaaagttt tgatatcggg ca 32

<210> 353

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 353

ctgaccctct cttaatcaac gttatatgtt tt 32

<210> 354

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 354

ctaattcgct aatttgaatc ttagctcgtc ta 32

<210> 355

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 355

aaggaagttc ttcactacaa acaactcata aa 32

<210> 356

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 356

cttgtaactt ttgagtgtct gttaactgtt tt 32

<210> 357

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 357

aaattgtata aaagatcatt cttaggaccg ct 32

<210> 358

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 358

cacttgtcaa atttctgggt gaatatcatt g 31

<210> 359

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 359

ctttcaaaat aagtggagac tttggcacat 30

<210> 360

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 360

aaaaattatt gcatgttttg ctgctccttt tg 32

<210> 361

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 361

agtagattag aatcatgaat gagaataacg cc 32

<210> 362

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 362

tcagttatga cgttttcgca aaagaaaaat ta 32

<210> 363

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 363

gatatcagga atgggaggtg taactggat 29

<210> 364

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 364

ctagataaca aaaccatcct atggccaata cc 32

<210> 365

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 365

gatctataat aacccaaatg gcaatacaac cc 32

<210> 366

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 366

tatttgaatt gcttggaagg tgtaatggta at 32

<210> 367

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 367

ttcgatagtg tcagatcgag atccaagatt ta 32

<210> 368

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 368

atacttgcgt gaaaattatt gttgtagacg ta 32

<210> 369

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 369

tcagtctgta aataaggatt tgcaatgtac c 31

<210> 370

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 370

attacttcgc cattatgaaa atagggtcat c 31

<210> 371

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 371

cgttagatct tcttcttcgt tctacatact tt 32

<210> 372

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 372

ataactcaaa tctttcaact aagtcgtgag tc 32

<210> 373

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 373

ttcctcgtac agcaaaggga aataatttaa tc 32

<210> 374

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 374

tttttgaagt gattgctaaa atcgtgatag ga 32

<210> 375

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 375

tcactttgat aggtaccact attcgattac at 32

<210> 376

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 376

atatcaatat taaccctatt catgatgcga cc 32

<210> 377

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 377

acattgatgc ttgacctaaa gttgtaagta tt 32

<210> 378

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 378

aaattgggtt tgattgcaac atatttcata gg 32

<210> 379

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 379

gctttctatc tctttttctc atgctattct ca 32

<210> 380

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 380

ccaatatatt caacatgtgt agttctggac tt 32

<210> 381

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 381

attttaataa agacacaagg gacaaagttc ac 32

<210> 382

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 382

tttgtgatta tgttttgtag tttatgtgcc tg 32

<210> 383

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 383

tcttgaggtt tatcaatcca atttctaagg tg 32

<210> 384

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 384

tcaatttgtc tttctaccca aattgtatca ac 32

<210> 385

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 385

tttatgttac tcatcaagta gagttcttac cg 32

<210> 386

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 386

agttatatat ttcaagactg gtccagacac t 31

<210> 387

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 387

agtcttagaa cttaggctat tctgacaccg 30

<210> 388

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 388

tgctgggttg aagaaaaata tccaaataca ta 32

<210> 389

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 389

gaggtagttt gctaaaacaa caacataaca tt 32

<210> 390

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 390

gtggttgtag tatatggaag atcaaaatca ca 32

<210> 391

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 391

cttgttccct tctctattgg tgttctcaat ac 32

<210> 392

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 392

cacttcatta tatgcattcg attttcttcc ac 32

<210> 393

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 393

cttaatttta ctctacgaaa tggtccccaa at 32

<210> 394

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 394

ataaagaaac tctcaggtgc aaatcgttta tc 32

<210> 395

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 395

acttgttaat tagatccact tggttacatc tc 32

<210> 396

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 396

aaatctaata attggagaat caagatggtg gg 32

<210> 397

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 397

gattggataa atgaaaagtc ttgttttcgg ag 32

<210> 398

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 398

aaagttctat catttcatca ctgcatctca aa 32

<210> 399

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 399

aaaaaccaga acctaaccat taaaccatag aa 32

<210> 400

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 400

aattgcgaaa tcaatattcg tcctcttata ca 32

<210> 401

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 401

attggcgcat ttcctttatg aaattttaga tg 32

<210> 402

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 402

gtctagaagt atctaatcat ttctcgagtg tt 32

<210> 403

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 403

ccttctactt ttagtacatc acaaactcaa ca 32

<210> 404

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 404

actcatttgt attcattgta attcattcgt gc 32

<210> 405

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 405

gtaacatatc ttccgatatt tagaaccttg gc 32

<210> 406

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 406

caaattagga attgatagca cctaagaggt tt 32

<210> 407

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 407

tttttaatat tgatgggact tgggctacta ca 32

<210> 408

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 408

gcccaaataa aggagatcat tgaaacaatt c 31

<210> 409

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 409

ctattaattg ttgttgccag atttttacct cc 32

<210> 410

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 410

tcaaataatt gtagagtagt ttgttcgtgt gt 32

<210> 411

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 411

atacttgtct aagtagtaga accactgtca tt 32

<210> 412

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 412

agatgctatg attcccttca atatcctctt at 32

<210> 413

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 413

gattcaaaga tagccttaat gttggtttgt tc 32

<210> 414

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 414

atttttgtaa ggtgcatatc catttctttc ac 32

<210> 415

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 415

atattttata tttacattcg catctccaag gt 32

<210> 416

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 416

gcatctttac gtttgaaagt cctatttgtt ag 32

<210> 417

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 417

agactttata tgttttcgag tgggattatg tg 32

<210> 418

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 418

tgggttttga acataattgt tttggtaact tt 32

<210> 419

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 419

taagaaaaac tttggagatc ttttcagagc aa 32

<210> 420

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 420

tcagatctag gaaaatttat ggaagacact ca 32

<210> 421

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 421

gttttgattc tcactattgt tcactttcac ac 32

<210> 422

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 422

aagcttcttg aatttagttt cgatgaaatc ag 32

<210> 423

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 423

gtaattcggt ctatgatgcc cttaaagtta tt 32

<210> 424

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 424

caaggtaatg gtggtgattt taggttataa gt 32

<210> 425

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 425

attttccatt aggatatttg acactatcac cc 32

<210> 426

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 426

tttgattgat atagctgata taggtgtttg cc 32

<210> 427

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 427

agcttttagt taatcactat ccttctctct ct 32

<210> 428

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 428

aaattggaga gtttgaagcc aattcaacag 30

<210> 429

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 429

ccagaaagat ggaaaaagtt tgaaagatta cg 32

<210> 430

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 430

tttactctgg agtatagaca aggtttttgg ga 32

<210> 431

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 431

aacaagattc aaaaattctc aactagcaaa gc 32

<210> 432

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 432

acgtgagtat gaagatacaa ttactttcac tg 32

<210> 433

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 433

tctcactaaa tctcttctct tctatctctt ca 32

<210> 434

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 434

tatataaagt cataacccat tcaacaccca ac 32

<210> 435

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 435

gaaaaggggg ttattacaga ttttgtctcc aa 32

<210> 436

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 436

tactctattt attctctctc tctctctctc gt 32

<210> 437

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 437

aggtttgaga gctgaaagca atttaagtat aa 32

<210> 438

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 438

cagtaagaaa tgaatatgaa aacgatgagt gt 32

<210> 439

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 439

tctaaaaagt gaatcatatt gaccccttaa ct 32

<210> 440

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 440

cattttagtc acatattgtt tcttaacagc cc 32

<210> 441

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 441

tagtgatatt tgggggtgtt ggttaggaat 30

<210> 442

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 442

aaatgaaaat acaatggtgt tcgacattag c 31

<210> 443

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 443

atcaataaat ggtccaaatt aaagttcgtg tg 32

<210> 444

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 444

aaaaagaaag aaaagctcac cttcactctt g 31

<210> 445

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 445

taacaaaata tgcaacaata tccacgaaat cc 32

<210> 446

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 446

atttgttgtt caagatgtgt atttgattgc tt 32

<210> 447

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 447

caataggtca aaggaggtat tagaattggt tc 32

<210> 448

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 448

tttgtttctg ccttaagtac tctgaagttt tt 32

<210> 449

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 449

gcaagcacat agatacaagg tatcattcat aa 32

<210> 450

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 450

tacacataag tagcttataa acttgggctc ta 32

<210> 451

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 451

aaatttttgt aaatgctctc aaccaatcac at 32

<210> 452

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 452

cataaggatt tgatcccatt ggacttgtct at 32

<210> 453

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 453

catttatctc atttaggcca aaaatatccc tg 32

<210> 454

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 454

tttttatttt tcttcaagat ggaaccctac ca 32

<210> 455

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 455

cagtcaccgg taaaatgttt tatttaaaga gg 32

<210> 456

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 456

acataatctg gatgtctaat tggtgttttc at 32

<210> 457

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 457

tctagtcata gaattgacta aattagggga cc 32

<210> 458

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 458

aaaatagttt gctacaaaac ttaggagtta gc 32

<210> 459

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 459

tgtgttaatt aagtacctaa aacatgatgc ct 32

<210> 460

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 460

gaggtaaatt tgtcgctcct gattattctt tt 32

<210> 461

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 461

ctctacgaaa actgcaaagt taatagagtc ag 32

<210> 462

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 462

tgtttcatgg aaaatgaatt gcttgatagt tt 32

<210> 463

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 463

ggtcaattca aacaaaactt tatcccgatt tt 32

<210> 464

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 464

gtattgttta ttcaaatgca caaacccact tg 32

<210> 465

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 465

tactacttgt aattgttgtg gtatgaaagg tc 32

<210> 466

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 466

ctcaacgaat tcctctaatt taactccatc aa 32

<210> 467

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 467

gagatgcagc aaaagaggta taatttatct ga 32

<210> 468

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 468

tatggtaatc cgcaaacaac aaacaacaaa ta 32

<210> 469

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 469

ttccacttca cactacagtg gctttac 27

<210> 470

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 470

taaaccgatg ttgaatcata tgactactga cc 32

<210> 471

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 471

gacatgatcc accctatttt ctattattgc at 32

<210> 472

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 472

tactttaatc ttcgtgttaa aaggtgaagt cc 32

<210> 473

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 473

caagaaaatg actacttcca atcttcttct ca 32

<210> 474

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 474

cactgatata accaaactgg ctacaatata gg 32

<210> 475

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 475

aaatgcaaca caagaatagt gagaaaagat tg 32

<210> 476

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 476

catactcact aacatagcaa tcagcgtgtc 30

<210> 477

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 477

ggttctgatg taggtcaaaa ataggaaaaa ct 32

<210> 478

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 478

attgacaaaa gaaaaacacg aatcaaagca aa 32

<210> 479

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 479

acagttgtat tccaggtaaa aagttaaaaa cg 32

<210> 480

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 480

cagattttac agtgtcgtca cataattcga g 31

<210> 481

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 481

taaaagaagg ctaggacttg ataacatagg ag 32

<210> 482

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 482

gctcttacac ttgttaattc ttttcaagca ag 32

<210> 483

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 483

gtgtgttttg tgtccttaca actcattttt c 31

<210> 484

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 484

aagttaaaag ttatccccct ccaaaagtta tc 32

<210> 485

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 485

aaatagactt tactgagaaa caaccaacag ag 32

<210> 486

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 486

tgtgaaaagt acaatcaaca tatcaacaaa gc 32

<210> 487

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 487

tgagtatgtc aaacattagc ataacaactc at 32

<210> 488

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 488

aaaatttcaa gaaaacaatg ccactagatc ac 32

<210> 489

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 489

tatagaaagt tctcacacag gaaattattg gc 32

<210> 490

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 490

tataggttac ttgatcttgc tgctaatttt gg 32

<210> 491

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 491

aataacgaac tatattttcc ctcgtttgtt cc 32

<210> 492

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 492

cttgtctttc tctctcttct tgtcgtg 27

<210> 493

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 493

cagtagtatg aggcctgaag tctcg 25

<210> 494

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 494

atccgtctct ctctctctct caatgactaa 30

<210> 495

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 495

tttgttgtca tatgatttca tttttgaagc ga 32

<210> 496

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 496

gaattgtgca taagctgata tggaaattaa cg 32

<210> 497

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 497

gtttttggtg atggtttatt tggtaagtca at 32

<210> 498

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 498

aaaaacagac aaacttgttc aataaaagac t 31

<210> 499

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 499

gaggggagct atgtctaaca accat 25

<210> 500

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 500

tctaactacc tcctcttaaa taacttctga cg 32

<210> 501

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 501

tatggcaact tgtaaggagt ttgaagatat tg 32

<210> 502

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 502

aaaagtaatg gtagtatttt ccttcttctg ct 32

<210> 503

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 503

ggtgagcaaa atataattct tacagccatt tc 32

<210> 504

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 504

ttatgtttat agtcattgtg attcagcatt cc 32

<210> 505

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 505

gattgctttt aaataaatgc ctagtttacc ct 32

<210> 506

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 506

ctctcatcag ttgccctgca tatcaat 27

<210> 507

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 507

cacaatctag gcttgatctg atactgtatt tc 32

<210> 508

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 508

atgatccaaa cgtaccttta agatttagtg tc 32

<210> 509

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 509

taaactcaat acaacatagc aagatccaaa at 32

<210> 510

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 510

tagtcagcaa catgttatcg ctttgg 26

<210> 511

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 511

agtttgaaaa ctgagaagaa gtaaattgag ga 32

<210> 512

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 512

atataaagtt agcctttctt tcattcaacg gt 32

<210> 513

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 513

acggtacgtt cagatatcta gagagataaa at 32

<210> 514

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 514

taggtcaacc acctaatcac tgacacaa 28

<210> 515

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 515

atttttctat ccaactatcg tagtccctcc tc 32

<210> 516

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 516

atctattcag gattccttag aagtttacac cc 32

<210> 517

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 517

gcttttcctg caaattttca acccaaaaa 29

<210> 518

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 518

acatacaaat agatattacg taggcttctc ct 32

<210> 519

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 519

aaatgaaaca aaggagagag attgtgaaac at 32

<210> 520

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 520

ttgtttatct taaacctctt tgtcatcatc ca 32

<210> 521

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 521

cagggtttcg tactcaacca ggta 24

<210> 522

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 522

aaaaatggaa actgaaacgg aaatcggtag ta 32

<210> 523

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 523

tgggaataaa gactctcttg ttaaaaactc aa 32

<210> 524

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 524

taaataaatc catcagcggc ctatgcttac 30

<210> 525

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 525

attggtattt ctcaaatttt aggagctgac at 32

<210> 526

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 526

ctaatctata gccctaacgt tctcaagaca ag 32

<210> 527

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 527

cttgtctttt cttttctccc aatctttgat tc 32

<210> 528

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 528

tcaaaattaa tgagcactaa tacctccttg ac 32

<210> 529

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 529

cttcatattc ggctttatta ttggtgcctt ta 32

<210> 530

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 530

gaaatggaaa ctaccaagga acaaagttca g 31

<210> 531

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 531

ttaaaactta cctgcttctt cattacatgt gc 32

<210> 532

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 532

aggattatct agtttgctca tttttcaaca ct 32

<210> 533

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 533

tgatcattaa gcgtttaaaa catgataagt tg 32

<210> 534

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 534

ttattcaaaa atcctaacga gggagataag tg 32

<210> 535

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 535

tttaagtgtt cacattcctt tctcttccaa tc 32

<210> 536

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 536

aaggaaataa atatcaaaat tccaaagcgc aa 32

<210> 537

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 537

cgaagtctaa ttatccctta actatacctc ct 32

<210> 538

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 538

ctccaaggga tccgattacc tctac 25

<210> 539

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 539

ccaaatctga tattgctatg taaagtcgag ag 32

<210> 540

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 540

tctataacac aaagaatcaa tacccaacag tc 32

<210> 541

<211> 28

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 541

cttcaagaga gttcgtgaat ttgcaacc 28

<210> 542

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 542

gtgtaaagag aggatcaaca atgaatgtga g 31

<210> 543

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 543

catacttcga aataagtgat tctcaaggct c 31

<210> 544

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 544

ggcttgtaag tacagaacca agtaaacatt 30

<210> 545

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 545

caacaagttt atcttttctg aacgggttat tg 32

<210> 546

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 546

gtttactcaa ttctgatgtt atgtgttttg gc 32

<210> 547

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 547

tatcgagtga cgtctgattc aagttatttt ac 32

<210> 548

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 548

taatacacaa atcatgtagt agcaagaaaa gc 32

<210> 549

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 549

gtcgacttag agatgaatct gatctttctg g 31

<210> 550

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 550

ggaaaattgg acagtgaatg tttactcttt tt 32

<210> 551

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 551

taaaagtgcg ttgatagctt ttggagataa ta 32

<210> 552

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 552

cttattaatc cgaagggacg tttttctatt ca 32

<210> 553

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 553

tttttcggtt tgttcgattt tctcgattct t 31

<210> 554

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 554

cgtttggagt ccgctagaaa actataataa a 31

<210> 555

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 555

taatacataa atctctccct ctcctttctc tc 32

<210> 556

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 556

caatcacttc attttactaa acagccaaac tt 32

<210> 557

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 557

tcttttaaac aaaggcccag tagatttgtt ag 32

<210> 558

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 558

ttatctggta ttacgcctct tttaccttat ca 32

<210> 559

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 559

ttgcatcatc aatataagga ataagctaag gg 32

<210> 560

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 560

ctaaattagg acatattttc tctaggcagt tg 32

<210> 561

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 561

tatcaaacag tagtatttgg agtttgattg gg 32

<210> 562

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 562

ctgtcacaga tacacagaga gaaacaaatt at 32

<210> 563

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 563

gaaaaatata ggagtaataa ataccgaccc cg 32

<210> 564

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 564

tatttttgta gttgtaccta tgttttgggt ga 32

<210> 565

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 565

gaccctatat ttcgttagga taagttggta ga 32

<210> 566

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 566

tttgatcgag tcttttctta gttagaatca cc 32

<210> 567

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 567

taaactttta tttggtttta aatagggcca cc 32

<210> 568

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 568

caaaattgat gtgttttatt ttgctggact ct 32

<210> 569

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 569

aatatctccc tttaatagtt tcagaaccgc at 32

<210> 570

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 570

aaaaagagag gggatatttg gagtaccaag a 31

<210> 571

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 571

ttcatgattt agtttctgaa taacccggta tc 32

<210> 572

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 572

gagttgcatc tcaagctagt tcgatttaaa ag 32

<210> 573

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 573

tacatatact aaccatggat ctctctctct ct 32

<210> 574

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 574

agtacaagta tggaattaat acctcacaaa gc 32

<210> 575

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 575

gaagagaaac tttgtatgaa tttgtaacgc tg 32

<210> 576

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 576

ttgttataat tttgtgaaac ttgtactggt gc 32

<210> 577

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 577

ccattgaaga tgttgccatt attcaaatac g 31

<210> 578

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 578

taaacttaat cgctttagcc gtttaacaaa tg 32

<210> 579

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 579

tattttacag tcaaagagag cgaaaagatg at 32

<210> 580

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 580

atattttgat ttttgcctct ggaactatga ct 32

<210> 581

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 581

tcttcatgaa atgttttcgt atatctgttt gt 32

<210> 582

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 582

atctgttcta taataagctt ctacctctgc at 32

<210> 583

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 583

aatgtattgc tcttgtcttt attgggttgt aa 32

<210> 584

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 584

acattgaaga aaatcttaaa atccctccat ct 32

<210> 585

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 585

cctacaaatc ttacacgaac tacctgaaat aa 32

<210> 586

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 586

ataaaattat gcattcattc gtttcatcgg tc 32

<210> 587

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 587

ttaaatatta aagtcatgct caacgcactt tc 32

<210> 588

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 588

aaaatatacc cttcctagtg atggatctaa gg 32

<210> 589

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 589

ctacaatgaa agcaaccacg acaatgaaac 30

<210> 590

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 590

tgttgaaggt aggaatttat gttgttatcg ag 32

<210> 591

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 591

gagtattata cccctgaaat cacaaacatt ca 32

<210> 592

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 592

tacccttcta gaaatgcaac tggtttataa tg 32

<210> 593

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 593

gcttgaggtc aatatttaga tcttcttctt ct 32

<210> 594

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 594

cttcccatca acatatttta tccacttctg at 32

<210> 595

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 595

cgacaaccat attcaggtgg atattaattc tt 32

<210> 596

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 596

gctttatctg aatgatctga ataagcatct ct 32

<210> 597

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 597

ttaatgttca tttctctctc atctaaacca cc 32

<210> 598

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 598

taattgatct acagcataca cgtttgtgtt tc 32

<210> 599

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 599

caagaaattg gaatgaaaat atgtagtggt gc 32

<210> 600

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 600

ctttctatag gtgttccaag tacccaaaat ag 32

<210> 601

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 601

gaaatcagtt taaaacctgg gaaaccatgc 30

<210> 602

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 602

taccacattt acaatttcag ttttgtctcg aa 32

<210> 603

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 603

gtaagattaa cgctgaaaaa tcagagagag ag 32

<210> 604

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 604

tttcaatcag caacagctga ccctac 26

<210> 605

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 605

tatacgtcaa aatttcccgt ccttttaatt ct 32

<210> 606

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 606

cttttgtatg aagaagacaa caatgatcca ac 32

<210> 607

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 607

ttatgtagtc tgaattgttc atttagctca cc 32

<210> 608

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 608

atcaaaacta tgcaatttgt taggcttctc ta 32

<210> 609

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 609

gctcttttat gagctttcct tttgttctta tt 32

<210> 610

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 610

catacacaat atgattgcaa agcatacatg ac 32

<210> 611

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 611

gttatgaatg aatggttgtg ataaaaacgg ac 32

<210> 612

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 612

aggaggaaga gaagaaaaat ctactatact ca 32

<210> 613

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 613

caagtcctat gagtatggtt gagtcagtc 29

<210> 614

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 614

tatgtagaga gagtgtatct gttcttgata gc 32

<210> 615

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 615

ttattcgagg tattcaggtt aataagtcaa gc 32

<210> 616

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 616

tttatatttt tgtgacagta actcagactt gt 32

<210> 617

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 617

ccttctttca tcattttctt tctttttcca gg 32

<210> 618

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 618

tgatttttct tttgtgttgt gatcttttgg aa 32

<210> 619

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 619

atttgcctac tatttttcag cctatttctt gt 32

<210> 620

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 620

atctcttcat cggtcaattt gtgtctattt tc 32

<210> 621

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 621

aatttatttg cgtgttttaa tgtcaacctt ct 32

<210> 622

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 622

atttgtacag tcctactgtc ttctaacatt tc 32

<210> 623

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 623

catttgaaga ggccattgaa atctacacag 30

<210> 624

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 624

taaacgagat tggtcactaa taatccaaac aa 32

<210> 625

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 625

cagctgtagt ttagaacttt agatccatca tc 32

<210> 626

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 626

gcatatgcaa actctaaaat tcgtctcctt at 32

<210> 627

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 627

caaggagatc aatctacccg accctac 27

<210> 628

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 628

catttcgtcg aacaacatat acacaacttt ta 32

<210> 629

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 629

catcttaggt agactattcc tatctactgc aa 32

<210> 630

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 630

caaactagag ccctcttaat tgaattcttc at 32

<210> 631

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 631

tttaccatca tctactataa gggaatctac gg 32

<210> 632

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 632

cataatctcc acagttatat cagcatcagt tc 32

<210> 633

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 633

tatatagctg ctctcttgac ttgtctaagc tc 32

<210> 634

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 634

acaattttga agaaaagtat cacctacgct at 32

<210> 635

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 635

aaaacgtttg acagttcctt gaaccatttg 30

<210> 636

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 636

actgatgtaa ttagattcat tcgcagaaac at 32

<210> 637

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 637

atggaaaaat cgaaaacaga aaccctaatt tc 32

<210> 638

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 638

ataatttatt tttctttgct ttgctttcct gc 32

<210> 639

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 639

aatcaatgga ttttgggttt cagtgagaga g 31

<210> 640

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 640

tatctatact ataacatatt tgaccggacc cc 32

<210> 641

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 641

atgtggattt tacctttaca aacgatgtag tc 32

<210> 642

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 642

tgtttgacct ctgatttagt tctttaagat ga 32

<210> 643

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 643

caacttccat taaacccact gtccc 25

<210> 644

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 644

cagtttgaaa acctagtgca accctc 26

<210> 645

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 645

gattacttaa aaattgcaga ccataacact cc 32

<210> 646

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 646

actagtttgt catgcattac ttataaatcc cc 32

<210> 647

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 647

tcatgtacta tgaaaagagg agagaacgat aa 32

<210> 648

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 648

ccttttcagt caaatttttc catgtattgt gt 32

<210> 649

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 649

ccaagtcaag caaaagttta tgtggatatt ct 32

<210> 650

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 650

tttatgcacc ataagtatgc tatttcatct cc 32

<210> 651

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 651

ctatggttct taggcagact ttcaaactta ac 32

<210> 652

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 652

atgagaattg atgaataaag agtgcaaaac ag 32

<210> 653

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 653

ttttaagctt gtgtttttca ttttccactt ct 32

<210> 654

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 654

aaaagtatcg aaatggaggc tttcaaaaat tg 32

<210> 655

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 655

ttaatctctc tctctctctc tctctctctc ta 32

<210> 656

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 656

caaaaatttg gaataacgac gtcaccttc 29

<210> 657

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 657

gctgtagtag agagatgttg agatctattt tg 32

<210> 658

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 658

tattttataa cggaaatact tgcatatggt cc 32

<210> 659

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 659

ttgatttctt tcttccatca ctcttgattt ct 32

<210> 660

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 660

aaaaggttca aaattctaaa aatgtgtctt gc 32

<210> 661

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 661

catccatgat gaacaaaata gagagtttag ga 32

<210> 662

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 662

acttatattt gatagcgcct agaatgattt cc 32

<210> 663

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 663

tctctttact ttatctttag cctcctatct cc 32

<210> 664

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 664

aaaaatacta tattgtctat agcggtcggc 30

<210> 665

<211> 30

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 665

atgactccac aaagtgaaaa gactcctaga 30

<210> 666

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 666

taacgatttt cgtaatataa cccttctggt tc 32

<210> 667

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 667

agaattgaag ggaaaagaaa cgtttataag ga 32

<210> 668

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 668

atacagttgt ttgttttaat aaatccgagt gc 32

<210> 669

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 669

catgtttcca attaaaaacc atttaccctt gt 32

<210> 670

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 670

aatactgaat ggatactaaa acgtgcctta tc 32

<210> 671

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 671

tttgagattt cttaacagtg gacatcatca tt 32

<210> 672

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 672

atataatgtt ggattcgatt ttggcatttg tt 32

<210> 673

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 673

accctgttct gaagcttttt aactaaatgt at 32

<210> 674

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 674

taaaatgtga ttgatgctac tttttagagg ct 32

<210> 675

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 675

cttattcagt ggtcaaatta gaagatgcta ca 32

<210> 676

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 676

ttaaaatatt ctaggtcatt ggatcaaggc tc 32

<210> 677

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 677

atttacattg aaaacacgac ttgcattgtc ag 32

<210> 678

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 678

ggcatctata aaaacccatg aacaaaaatg at 32

<210> 679

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 679

ctacttgtat agtttttcaa gtcgtgtcat cc 32

<210> 680

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 680

taataaattt tacaattgtt tgaggcgagt cc 32

<210> 681

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 681

agaagtatca gctatcatta catttgtatc gc 32

<210> 682

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 682

tatatcgcct tgtcattgtt cattttctta gt 32

<210> 683

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 683

tatttcttta ttctcttctg gatggtctga ct 32

<210> 684

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 684

cttaaaaatt ccctcaactt tttcgtcctt ag 32

<210> 685

<211> 29

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 685

cctcgaacta ggctcataat cttcttgtt 29

<210> 686

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 686

ggaatgatgt ccgaattaga cagatttact at 32

<210> 687

<211> 31

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 687

aaaaatttgg tgtaggctaa aatcaaagct g 31

<210> 688

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 688

atctatccat tactacaaga ctctaaccac ac 32

<210> 689

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 689

tcatcctgag tcataacatt gaactctttt tc 32

<210> 690

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 690

ttctacttcc ttttctatag tgcatgtcta gt 32

<210> 691

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 691

gtttattact acttcgtgac ccatacactt ac 32

<210> 692

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 692

cccatagcta tcctttggtc atctagc 27

<210> 693

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 693

attcaaaaac caaactattc ctgtcaattc ag 32

<210> 694

<211> 32

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 694

aaactgattc tgaaaattgt aaccaaacag ag 32

Claims (5)

1.猕猴桃品种分子鉴定的MNP核心引物组合，所述的引物组合为位点编号MHT0001~MHT0347对应的347对引物，具体的引物序列如说明书表1所示。

2.权利要求1所述的引物组合在构建猕猴桃DNA指纹数据库中的应用。

3.权利要求1所述的引物组合在检测猕猴桃品种真实性中的应用。

4.权利要求1所述的引物组合在猕猴桃种质资源遗传多样性分析中的应用。

5.权利要求1所述的引物组合在猕猴桃分子育种中的应用。

Images