CN114424712B - Quick breeding method for roxburgh rose in North China - Google Patents
Quick breeding method for roxburgh rose in North China Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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Abstract
The invention provides a method for rapid breeding of roxburgh rose in North China, and belongs to the technical field of plant asexual propagation. The method for fast breeding the roxburgh rose in the North China comprises the following steps: introducing fructus Rosae Normalis from Huang-Huai line in south, transplanting to North China, performing cold-resistant domestication, and continuously overwintering, and screening cold-resistant fructus Rosae Normalis with strong cold resistance and capable of overwintering in North China; selecting cutting slips before dormancy of the cold-resistant roxburgh rose, soaking rooting powder solution, and carrying out cutting management after infection of agrobacterium rhizogenes. The cutting method has the advantages of high survival rate, fast germination and rooting, easy management and protection, suitability for use in North China, and the like, so that the Rosa roxburghii can safely overwinter in North China.
Description
Technical Field
The invention belongs to the technical field of plant asexual propagation, and particularly relates to a method for fast propagation of roxburgh rose in North China.
Background
Rosa roxburghii (Rosa roxburghii Tratt.) belongs to the family Rosaceae, and has been widely used as an edible and pharmaceutical resource in Asian countries. Because of rich vitamin C content and unique flavor, the beverage can be used for producing various beverages, jams and canned foods. Fruits contain various bioactive components such as organic acids, flavonoids, polysaccharides and triterpenes, and fruit juice is consumed in the folk as a delicious beverage and herbal tea, and has the functions of tonifying spleen, treating diarrhea and clearing summer heat. Is suitable for people suffering from hypertension, hyperlipidemia, hyperglycemia or inappetence; in addition, the 'compendium of materia medica' records that the roxburgh rose root soup can treat diarrhea, stomachache, chronic gastritis and acute enteritis of people and animals; the leaves can be used for preparing Chinese tea and effectively treating dyspepsia.
Rosa roxburghii is widely distributed in the areas of Shaanxi, gansu, jiangxi, anhui, zhejiang, fujian, hunan, hubei, sichuan, yunnan, guizhou, tibet and the like in subtropical areas of China, and also in Japan. In China, the distribution area of Guizhou, sichuan, yunnan, shaanxi, hubei and Hunan is large, and the yield is high. Ao Qin and the like are used for researching the suitability of the Guizhou roxburgh rose climate, and by observing sample points, the optimal growing area of the roxburgh rose is obtained, the area has good heat condition and good humidity condition, the altitude is 800-1600m, the average temperature is 18.0-21.0 ℃ in 5-8 months, the average temperature is 19.5-23.5 ℃ in 7 months, the climate is mild, the rainwater is sufficient, and the climate is warm in summer; early drought easily occurs in the region of the suitable area, and the growth and development of the Rosa roxburghii fruit are limited; in the secondary adaptive area, insufficient heat, less precipitation and overhigh temperature are unfavorable for the growth and development of the roxburgh rose. It can be seen that temperature and precipitation are the main causes of the growth of Rosa roxburghii. The growth speed of the roxburgh rose is slow, especially in North China with little precipitation and colder climate, so a large number of roxburgh rose seedlings can be obtained quickly by breeding the roxburgh rose in a cutting seedling raising mode. However, the traditional cutting method of the roxburgh rose in North China is easy to cause the phenomena of seedling death, root rot and the like of the roxburgh rose, has low survival rate and prevents the asexual propagation of the roxburgh rose. Therefore, the cutting method of the roxburgh rose is provided to overcome the restriction factors such as unfavorable climatic conditions in North China, and is an important technical means for ensuring the cutting rooting rate of the roxburgh rose and the safe overwintering of cutting seedlings.
Disclosure of Invention
Therefore, the invention aims to provide the method for rapidly breeding the roxburgh rose in the North China, and the winter survival rate of the roxburgh rose cutting seedlings in the North China is improved to be more than 95% through cold domestication, rooting liquid treatment, agrobacterium rhizogenes inoculation and cutting management.
In order to achieve the above object, the present invention provides the following technical solutions:
a method for rapidly breeding fructus Rosae Normalis in North China comprises introducing fructus Rosae Normalis from Huang-Huai line in south, transplanting to North China, performing cold-resistant domestication, continuously overwintering, and screening cold-resistant fructus Rosae Normalis with strong cold resistance and capable of overwintering in North China; selecting cutting slips before dormancy of the cold-resistant roxburgh rose, soaking rooting powder solution, and carrying out cutting management after infection of agrobacterium rhizogenes.
Preferably, the cold-resistant domestication is that after the roxburgh rose plants are transplanted to North China in spring, the cold-resistant domestication is carried out on the roxburgh rose plants by utilizing the weather with the average temperature of less than 10 ℃ in winter in North China, and plants capable of continuously overwintering are selected as cold-resistant roxburgh rose plants.
Preferably, the cutting is a current year semi-lignified branch or a tender branch.
Preferably, the cutting is a short branch of 2-6cm, the morphological upper end is horizontally sheared near the bud point, and the morphological lower end is obliquely sheared near the bud point.
Preferably, the rooting powder soaking solution is prepared by soaking the cutting root parts in the rooting powder solution for 2-4 hours.
More preferably, the rooting powder solution is an ABT rooting powder solution with the concentration of 0.08-0.12 g/L.
Preferably, the agrobacterium rhizogenes is agrobacterium rhizogenes K599.
Preferably, the cutting wood cutting matrix is coarse sand or perlite, vermiculite and nutrient soil mixed in equal volume.
Preferably, the cutting depth of the cutting slips is 1-2cm, and the bud points are exposed.
Preferably, the cutting management is to cover the transparent cover for the cutting, and culture the cutting in an environment with the temperature of 15-25 ℃ and the air humidity of 35-55%, and keep the moisture content of the cutting matrix at 30-60%.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a method for fast breeding roxburgh rose in North China, which comprises the steps of introducing and domesticating, and continuously overwintering and screening cold-resistant plants to improve the cold-resistant capability of roxburgh rose cutting mother plants; the cutting slip is selected before the cold-resistant roxburgh rose is dormant, the rooting inhibiting substances are less while the development of branches is full, and the cutting slip is further treated by using rooting powder solution and agrobacterium rhizogenes, so that roxburgh rose seedlings can be obtained rapidly in North China, the cutting survival rate of the roxburgh rose is greatly increased, the cutting slip rooting time of the roxburgh rose is shortened, the cutting slip can survive well after being transplanted outside the North China, and the survival rate is over 95%.
Drawings
Fig. 1: the basic flow pattern of the present invention.
Detailed Description
The invention provides a method for fast breeding roxburgh rose in North China, which comprises the steps of introducing roxburgh rose from Huang-Huai line in south, transplanting to North China for cold-resistant domestication, then continuously overwintering, and screening cold-resistant roxburgh rose with strong cold resistance and overwintering in North China; selecting cutting slips before dormancy of the cold-resistant roxburgh rose, soaking rooting powder solution, and carrying out cutting management after infection of agrobacterium rhizogenes. Dormant branches develop fully, accumulate a large amount of nutrients and are beneficial to producing adventitious roots, but also contain a large amount of rooting inhibiting substances, and have low endogenous auxin content. According to the invention, the cutting slip is selected before the cold-resistant roxburgh rose is dormant, and the rooting liquid treatment and agrobacterium rhizogenes infection are adopted, so that the cutting survival rate of the roxburgh rose is increased, and the cutting slip rooting time of the roxburgh rose is shortened.
The cold-resistant domestication is that after the Rosa roxburghii tratt is transplanted to North China in spring, the Rosa roxburghii tratt is subjected to cold domestication by utilizing the weather with the average temperature of less than 10 ℃ in winter in North China, and plants capable of continuously overwintering are selected as cold-resistant Rosa roxburghii tratt. The invention is not particularly limited to cultivation management after transplanting, and the method is only needed by adopting a conventional cultivation management method in the field. The invention improves the cold resistance of the mother plant of the Rosa roxburghii cutting by introducing and domesticating and continuously overwintering and screening cold-resistant plants, is beneficial to the cutting survival of cutting slips in the North China and improves the transplanting overwintering survival rate of cutting slips.
The cutting slip is preferably a current-year-old semi-lignified branch or a tender branch; further preferably selecting a parent plant with excellent growth state, and selecting current-year branches growing robustly on the parent plant for pruning to obtain cutting slips; more preferably, the cut shoots are 2-4 leaf shoots or no leaf shoots. The invention cuts the cutting spike from the current annual semi-lignified branch or tender branch of the cold-resistant mother plant of the roxburgh rose, thereby ensuring the cutting survival rate of the roxburgh rose in North China.
The cutting is preferably 2-6cm short branches, the morphological upper end is horizontally sheared near the bud point, and the morphological lower end is obliquely sheared near the bud point; more preferably, the length of the cutting pin is 5cm.
The preferred rooting powder soaking solution is to soak the cutting root parts in the rooting powder solution for 2-4 hours; further preferably, the soaking is carried out for 3 hours. The preferred rooting powder solution of the invention is an ABT rooting powder solution with the concentration of 0.08-0.12 g/L; further preferably, the concentration is 0.10g/L. As an implementation mode, the effective components of the ABT rooting powder solution comprise 30% indoleacetic acid and 20% naphthylacetic acid.
The preferred strain of Agrobacterium tumefaciens for the invasive dyeing of hair in the present invention is Agrobacterium rhizogenes K599. Further preferred modes of infestation include: the base of the cutting was infected with the activated Agrobacterium rhizogenes K599 strain wound using a disposable syringe. More preferably, agrobacterium rhizogenes K599 of the present invention is activated by: the agrobacterium rhizogenes is stored in a refrigerator at the temperature of minus 80 ℃ and is activated by a liquid culture medium; the liquid culture medium is as follows: 1g peptone, 1g yeast extract and 0.5g sodium chloride are poured into 100ml water and sterilized at 121℃for 15min, and 5-10mg Kanamycin (Kanamycin) and 5-10mg Rifampicin (Rifampicin) are added thereto; after the Agrobacterium rhizogenes was removed from the refrigerator and thawed, 200ul of the bacterial liquid was aspirated and added to 20ml of the liquid medium, and the medium was subjected to shaking culture at 28℃for 24 hours at 150 r. Agrobacterium rhizogenes is a gram-negative aerobic soil bacterium of the genus Agrobacterium of the family Rhizobiaceae, is rod-shaped in shape and flagellum, can infect most dicotyledonous plants, few monocotyledonous plants and individual gymnosperms, and can promote the cutting to produce adventitious roots which grow rapidly after the cutting is infected by the Agrobacterium rhizogenes K599.
The cutting medium is preferably coarse sand or perlite, vermiculite and nutrient soil mixed in equal volume; further preferably, the cutting matrix is mixed before cutting, and water is added into a tray to enable the cutting matrix to naturally absorb water and wet until the water content is 30-60%; more preferably the water content is 45%.
The cutting depth of the short branch cutting slips after the preferred treatment is 1-2cm, and the bud points are exposed.
The invention preferably slightly presses the matrix around the cutting slips after cutting, covers a transparent cover above the cutting slips, and cultures the cutting slips in an environment with the temperature of 15-25 ℃ and the air humidity of 35-55%, and keeps the water content of the cutting matrix to be 30-60%. Further preferably, the cutting temperature is 20℃and the air humidity is 45%, and the water content of the substrate is 45%. As an alternative embodiment, the moisture content of the substrate is continuously monitored, and water is timely added to the substrate to meet the moisture demand of the cutting slips.
The invention also comprises that new roots of the cutting shoots grow to 3cm to 5cm and can be transplanted; it is further preferable that the new roots are transplanted when they grow to 4 cm.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
A method for fast breeding roxburgh rose in North China comprises the following steps:
(1) Introducing fructus Rosae Normalis from Huang-Huai line in south, transplanting to North China, performing cold resistance domestication, and continuously overwintering, and screening out plants with strong cold resistance and capable of overwintering in North China; the cold-resistant domestication is that after the roxburgh rose plants are transplanted to North China in spring, the roxburgh rose plants are subjected to cold domestication by utilizing the weather that the average temperature of winter in North China is below 10 ℃, and plants capable of continuously overwintering are selected as cold-resistant roxburgh rose plants;
(2) Selecting a parent plant with excellent growth state before cold-resistant roxburgh rose planted in North China sleeps in winter, and selecting strong semi-lignified branches growing on the parent plant for pruning, wherein the pruned branches have 3 leaves;
(3) Cutting short branches of 5cm on each section of parent plant, and horizontally cutting the parts, close to bud points, of the morphological upper ends of the short branches; obliquely shearing the part, close to the bud point, of the morphological lower end;
(4) Activating agrobacterium rhizogenes K599; the agrobacterium rhizogenes is stored in a refrigerator at the temperature of minus 80 ℃ and is activated by a liquid culture medium; the liquid culture medium is as follows: 1g peptone, 1g yeast extract and 0.5g sodium chloride were poured into 100ml water and sterilized at 121℃for 15 minutes, to which were added 8mg Kanamycin (Kanamycin) and 8mg Rifampicin (Rifampicin); after the agrobacterium rhizogenes is taken out from the refrigerator and thawed, 200ul of bacterial liquid is sucked and added into 20ml of liquid culture medium, and the culture is carried out for 24 hours at 28 ℃ and 150r of shaking table;
(5) Soaking the base part of the short branch to be cut in an ABT rooting powder solution for 3 hours;
(6) Sucking the wound infection cutting base of the activated agrobacterium rhizogenes K599 bacterial liquid by using a disposable syringe;
(7) Preparing cutting matrix coarse sand before cutting, and adding water into the matrix to enable the cutting matrix to naturally absorb water and wet until the water content is 45%;
(8) Inserting the treated short branch into a basin, so that the lower part of the short branch is inserted into a matrix for 1.5cm, and the bud point is exposed;
(9) Slightly pressing the substrate around the cutting wood after cutting, covering a transparent cover on the pot, and culturing in an environment with air temperature of 20 ℃ and air humidity of 45%, so as to keep the moisture content of the cutting substrate at 45%;
(10) The new roots of the cuttings grow to 4cm and can be transplanted.
Example 2
A method for fast breeding roxburgh rose in North China comprises the following steps:
(1) Introducing fructus Rosae Normalis from Huang-Huai line in south, transplanting to North China, performing cold resistance domestication, and continuously overwintering, and screening out plants with strong cold resistance and capable of overwintering in North China; the cold-resistant domestication is that after the roxburgh rose plants are transplanted to North China in spring, the roxburgh rose plants are subjected to cold domestication by utilizing the weather that the average temperature of winter in North China is below 10 ℃, and plants capable of continuously overwintering are selected as cold-resistant roxburgh rose plants;
(2) Selecting a parent plant with excellent growth state before cold-resistant roxburgh rose planted in North China sleeps in winter, and selecting strong semi-lignified branches growing on the parent plant for pruning, wherein the pruned branches do not have leaves;
(3) Cutting short branches of 2cm in each section from the parent plant, and horizontally cutting the parts, close to the bud points, of the morphological upper ends of the short branches; obliquely shearing the part, close to the bud point, of the morphological lower end;
(4) Activating agrobacterium rhizogenes K599; the agrobacterium rhizogenes is stored in a refrigerator at the temperature of minus 80 ℃ and is activated by a liquid culture medium; the liquid culture medium is as follows: 1g peptone, 1g yeast extract and 0.5g sodium chloride were poured into 100ml water and sterilized at 121℃for 15 minutes, to which were added 5mg Kanamycin (Kanamycin) and 5mg Rifampicin (Rifampicin); after the agrobacterium rhizogenes is taken out from the refrigerator and thawed, 200ul of bacterial liquid is sucked and added into 20ml of liquid culture medium, and the culture is carried out for 24 hours at 28 ℃ and 150r of shaking table;
(5) Soaking the base part of the short branch to be cut in an ABT rooting powder solution for 2 hours;
(6) Sucking the wound infection cutting base of the activated agrobacterium rhizogenes K599 bacterial liquid by using a disposable syringe;
(7) Preparing a cutting matrix, mixing perlite, vermiculite and nutrient soil in equal volume before cutting, and adding water into the matrix to enable the cutting matrix to naturally absorb water and wet until the water content is 30%;
(8) Inserting the treated short branch into a basin, so that the lower part of the short branch is inserted into a matrix for 1cm, and the bud point is exposed;
(9) Slightly pressing the substrate around the cutting wood after cutting, covering a transparent cover on the basin, and culturing in an environment with the air temperature of 15 ℃ and the air humidity of 35%, so as to keep the moisture content of the cutting substrate to be 30%;
(10) The new roots of the cuttings grow to 3cm and can be transplanted.
Example 3
A method for fast breeding roxburgh rose in North China comprises the following steps:
(1) Introducing fructus Rosae Normalis from Huang-Huai line in south, transplanting to North China, performing cold resistance domestication, and continuously overwintering, and screening out plants with strong cold resistance and capable of overwintering in North China; the cold-resistant domestication is that after the roxburgh rose plants are transplanted to North China in spring, the roxburgh rose plants are subjected to cold domestication by utilizing the weather that the average temperature of winter in North China is below 10 ℃, and plants capable of continuously overwintering are selected as cold-resistant roxburgh rose plants;
(2) Selecting a parent plant with excellent growth state before cold-resistant roxburgh rose planted in North China sleeps in winter, and selecting strong tender branch growing on the parent plant for pruning, wherein the pruned small branch has 4 leaves;
(3) Cutting short branches of 6cm in each section from the parent plant, and horizontally cutting the parts, close to the bud points, of the morphological upper ends of the short branches; obliquely shearing the part, close to the bud point, of the morphological lower end;
(4) Activating agrobacterium rhizogenes K599; the agrobacterium rhizogenes is stored in a refrigerator at the temperature of minus 80 ℃ and is activated by a liquid culture medium; the liquid culture medium is as follows: 1g peptone, 1g yeast extract and 0.5g sodium chloride were poured into 100ml water and sterilized at 121℃for 15 minutes, and 10mg Kanamycin (Kanamycin) and 10mg Rifampicin (Rifampicin) were added thereto; after the agrobacterium rhizogenes is taken out from the refrigerator and thawed, 200ul of bacterial liquid is sucked and added into 20ml of liquid culture medium, and the culture is carried out for 24 hours at 28 ℃ and 150r of shaking table;
(5) Soaking the base part of the short branch to be cut in an ABT rooting powder solution for 4 hours;
(6) Sucking the wound infection cutting base of the activated agrobacterium rhizogenes K599 bacterial liquid by using a disposable syringe;
(7) Preparing cutting matrix coarse sand before cutting, and adding water into the matrix to make the cutting matrix naturally wet until the water content is 60%;
(8) Inserting the treated short branch into a basin, so that the lower part of the short branch is inserted into a matrix for 2cm, and the bud point is exposed;
(9) Slightly pressing the substrate around the cutting wood after cutting, covering a transparent cover on the basin, and culturing in an environment with the air temperature of 25 ℃ and the air humidity of 55%, so as to keep the moisture content of the cutting substrate at 60%;
(10) The new roots of the cuttings grow to 5cm and can be transplanted.
Example 4
Influence of cold-resistant domestication on wintering survival rate of cutting seedlings of roxburgh rose
The method comprises the steps of selecting the Rosa roxburghii plant which is subjected to cold resistance and domestication and is not subjected to cold resistance and is similar in growth vigor, cutting the Rosa roxburghii plant according to the method of the embodiment 1, and observing the influence of the cold resistance and domestication on the rooting rate and the overwintering survival rate of the Rosa roxburghii plant.
The result shows that the rooting rate of the roxburgh rose plant which is not subjected to cold-resistant domestication is not different from that of the roxburgh rose plant which is subjected to cold-resistant domestication, but the overwintering survival rate of the cutting seedling is only 10%, and the overwintering survival rate of the roxburgh rose cutting seedling which is subjected to cold-resistant domestication is 95%.
Example 5
Cutting control tests were carried out according to the cutting method of example 1 by selecting branches with leaves and branches without leaves and with development degree of semi-lignified branches and twigs and setting equal proportion of mixed matrix and coarse sand as cutting matrix, counting rooting rate, and comparing the influence of different conditions on the rooting rate of Rosa roxburghii cutting, and the results are shown in table 1.
TABLE 1 influence of different cuttings and substrates on cutting rooting Rate of Rosa roxburghii
As can be seen from table 1, the rooting rate of the cutting of the rosa roxburghii is affected by whether the branches carry leaves, the matrix composition and the development degree of the branches, and is expressed as follows: the mixed matrix with leaves and leaves is larger than coarse sand, the semi-lignified branch is larger than tender branch, wherein the branch is provided with leaves, the mixed matrix with equal proportion and the semi-lignified branch is used as cutting, the cutting survival rate is highest and reaches 91%, and the method is obviously superior to other cutting treatments.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (4)
1. A method for quickly breeding roxburgh rose in North China is characterized in that roxburgh rose is introduced from Huang-Huai line in south, transplanted to North China for cold-resistant domestication, and then continuously overwintered, and cold-resistant roxburgh rose with strong cold resistance and overwintering in North China is screened out; selecting cutting slips before dormancy of the cold-resistant roxburgh rose, soaking rooting powder solution, and carrying out cutting management after infection of agrobacterium rhizogenes;
after transplanting the roxburgh rose plants to North China in spring, carrying out cold domestication on the roxburgh rose plants by utilizing the weather with the average temperature of less than 10 ℃ in winter in North China, and screening plants capable of continuously overwintering as cold-resistant roxburgh rose plants;
the rooting powder soaking solution is prepared by soaking the cutting root in an ABT rooting powder solution of 0.08-0.12g/L for 2-4h;
the cutting slip is a current annual semi-lignified branch or a tender branch with the length of 2-6cm, the cutting slip cutting matrix is perlite, vermiculite and nutrient soil which are mixed in equal volume, the cutting slip cutting depth is 1-2cm, and the bud points are exposed outside.
2. The method for rapid propagation of Rosa roxburghii in North China according to claim 1, wherein the morphological upper end of the cutting pin is cut horizontally near the bud point, and the morphological lower end is cut obliquely near the bud point.
3. The method for rapid propagation of rosa roxburghii in north China according to claim 1, wherein the agrobacterium rhizogenes is agrobacterium rhizogenes K599.
4. The method for rapid propagation of rosa roxburghii in north China according to claim 1, wherein the cutting management is to cover a transparent cover for cutting spikes, and the cutting spikes are cultured in an environment with the temperature of 15-25 ℃ and the air humidity of 35-55%, and the water content of the cutting medium is kept at 30-60%.
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