CN112273383A - Plant growth regulator, preparation method and eucommia improved variety twig cutting seedling method using plant growth regulator - Google Patents
Plant growth regulator, preparation method and eucommia improved variety twig cutting seedling method using plant growth regulator Download PDFInfo
- Publication number
- CN112273383A CN112273383A CN202011236585.9A CN202011236585A CN112273383A CN 112273383 A CN112273383 A CN 112273383A CN 202011236585 A CN202011236585 A CN 202011236585A CN 112273383 A CN112273383 A CN 112273383A
- Authority
- CN
- China
- Prior art keywords
- cutting
- eucommia ulmoides
- plant growth
- growth regulator
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a plant growth regulator, a preparation method and an improved eucommia variety twig cutting seedling method using the plant growth regulator, and relates to the technical field of economic tree seedling cultivation. The plant growth regulator is mainly obtained by compounding naphthylacetic acid, indolebutyric acid, gibberellin and 6-benzylaminopurine according to a specific proportion, achieves the technical effect of synergistic interaction through the synergistic combination of the raw materials, and proves that the plant growth regulator can greatly improve the survival rate of the cutting of the improved variety twig of eucommia ulmoides; meanwhile, the eucommia ulmoides fine variety twig cutting seedling raising method utilizing the plant growth regulator breaks through the limitation of a single fine variety grafting propagation method adopted in the existing eucommia ulmoides production, accelerates the propagation speed of the eucommia ulmoides fine variety seedlings, and further relieves the urgent need of the existing eucommia ulmoides production on the eucommia ulmoides fine variety cutting seedlings.
Description
Technical Field
The invention relates to the technical field of economic tree seedling culture and cultivation, in particular to a plant growth regulator, a preparation method and an improved eucommia seed twig cutting seedling culture method using the plant growth regulator.
Background
Eucommia ulmoides (Eucommia ulmoides Oliv) is a special rare economic tree species in China, and is cultivated in 28 provinces (regions and cities) from subtropical zone to warm temperate zone in China, and the cultivation area is about 600 ten thousand mu. The eucommia bark is a special rare Chinese medicinal material and an important export foreign exchange material in China, has the effects of strengthening muscles and bones, tonifying liver and kidney, losing weight for a long time, resisting aging and the like, and is a top grade Chinese medicinal product. A large number of recent researches prove that the eucommia ulmoides fruits have extremely high economic value and utilization prospect, the pericarp (seed coat) contains chlorogenic acid, aucubin, geniposide acid, pinoresino disaccharide glycoside, polysaccharide, amino acid, Zn, Mn, Cu, Fe, Ca, P, B, Mg, K and other mineral elements, has pharmacological functions similar to those of eucommia ulmoides bark, and has no toxic or side effect; particularly, the eucommia kernel contains rich unsaturated fatty acid such as alpha-linolenic acid, wherein the content of the alpha-linolenic acid reaches more than 60 percent, and has the functions of obviously reducing blood fat, reducing blood pressure, inhibiting thrombotic diseases, preventing myocardial infarction, cerebral infarction and the like. Besides traditional medicine, eucommia ulmoides is also a high-quality raw material for producing various functional foods. The eucommia ulmoides tree is elegant in shape, developed in root system, rapid in growth, strong in stress resistance and less in plant diseases and insect pests, and is a good special economic tree species in hills, mountain areas and plain sand areas. The utilization of eucommia ulmoides fruits is more and more emphasized by people, the eucommia ulmoides fruits are mainly produced, and an orchard-oriented and gardening-oriented cultivation mode for comprehensively utilizing eucommia ulmoides leaves, barks, fruits and male flowers becomes one of main cultivation modes for the production and development of eucommia ulmoides.
Good varieties and propagation technology support are needed for high-quality and high-yield cultivation of eucommia ulmoides, 27 good varieties of eucommia ulmoides in China and China series have been bred through systematic research for more than 30 years, and a good resource foundation is laid for high-quality and high-yield cultivation of eucommia ulmoides. At present, all aspects give high attention to the development of the eucommia ulmoides industry, and the eucommia ulmoides industry has an unprecedented good development situation. However, the eucommia ulmoides fine variety twig cutting technology has no major breakthrough, and the survival rate of fine variety big tree twig cutting is only 5% -30%, so that industrialization cannot be realized. At present, most of improved varieties are bred by adopting a grafting method, the breeding time of one grafted seedling needs 2 years, and the healthy development of the eucommia ulmoides industry is seriously restricted by the problem of slow breeding speed of improved seedlings.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a plant growth regulator, which is mainly prepared by compounding naphthylacetic acid, indolebutyric acid, gibberellin and 6-benzylaminopurine according to a specific proportion, and the plant growth regulator is proved to be capable of greatly improving the survival rate of the cuttings of improved variety of eucommia ulmoides.
The second purpose of the invention is to provide a preparation method of the plant growth regulator, which has the advantages of simple preparation process and easy operation.
The third purpose of the invention is to provide the application of the plant growth regulator in the cutting seedling of the improved variety twig of eucommia.
The fourth purpose of the invention is to provide a cutting seedling method for improved variety twigs of eucommia ulmoides.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
the invention provides a plant growth regulator, which comprises the following components in percentage by mass:
15-35% of naphthylacetic acid, 35-55% of indolebutyric acid, 5-15% of gibberellin and 10-15% of 6-benzylaminopurine;
the sum of the mass percentages of the components in the plant growth regulator is 100%.
Further, the plant growth regulator comprises the following components in percentage by mass:
15-25% of naphthylacetic acid, 35-50% of indolebutyric acid, 5-12% of gibberellin and 10-13% of 6-benzylaminopurine;
the sum of the mass percentages of the components in the plant growth regulator is 100 percent;
preferably, the plant growth regulator comprises the following components in percentage by mass: 25% of naphthylacetic acid, 50% of indolebutyric acid, 12% of gibberellin and 13% of 6-benzylaminopurine.
The invention provides a preparation method of the plant growth regulator, which comprises the following steps:
the plant growth regulator is prepared by uniformly mixing the raw materials according to the formula ratio.
The invention provides an application of the plant growth regulator in cutting seedling of an improved variety of eucommia ulmoides twigs.
The invention provides a cutting seedling method for an improved variety twig of eucommia, which comprises the following steps:
providing an aqueous solution with a solute of the plant growth regulator as a cutting solution; then soaking the eucommia ulmoides twig cutting slips in a cutting solution to obtain soaked eucommia ulmoides twig cutting slips; and then inserting the soaked eucommia ulmoides twig cutting slips into a seedling raising bed for seedling raising culture to obtain the eucommia ulmoides fine variety twig cutting seedlings.
Further, the preparation method of the cutting solution comprises the following steps:
dissolving a plant growth regulator in a solvent to obtain a dissolved solution A; adding water into the dissolving solution A to dilute the dissolving solution A into a cutting solution of 200-1000 mg/kg;
preferably, the solvent is ethanol, preferably ethanol with a concentration of 75%;
more preferably, the solid-to-liquid ratio of the plant growth regulator to ethanol in the solution a is 1: 15-25 g/ml;
preferably, the concentration of the cutting solution is 200-1000 mg/kg.
Further, the soaking time for soaking the eucommia ulmoides twig cutting slips into the cutting solution is 5-120 seconds.
Furthermore, the seedling bed is mainly prepared from turfy soil and perlite;
preferably, the mass ratio of the peatmoss to the perlite is 2-4: 1, preferably 3: 1;
preferably, the thickness of the seedling raising bed is 15-20 cm.
Furthermore, the temperature of the seedling culture is 22-30 ℃, and the relative humidity of air is 80-98%.
Further, the eucommia ulmoides improved variety twig cutting seedling method comprises the following steps:
(a) and preparing a cutting solution: mixing plant growth regulator in a ratio of 1: dissolving 15-25 g/ml of solid-to-liquid ratio in ethanol to obtain a solution A; adding water into the dissolving solution A to dilute the dissolving solution A into a cutting solution of 200-1000 mg/kg;
(b) providing semi-lignified eucommia ulmoides twigs with the length of 8-16 cm and the base stem of 0.2-0.6 cm as eucommia ulmoides twigs cutting slips, and soaking the eucommia ulmoides twigs cutting slips in the cutting solution obtained in the step (a) for 5-120 seconds to obtain soaked eucommia ulmoides twigs cutting slips;
(c) mixing turfy soil and perlite according to the weight ratio of 3:1, uniformly mixing the materials in a mass ratio to prepare a seedling bed with the thickness of 15-20 cm, inserting the eucommia ulmoides twig cutting slips obtained in the step (b) into the seedling bed, performing seedling culture in an environment with the temperature of 22-30 ℃ and the relative air humidity of 80-98%, and obtaining the eucommia ulmoides fine variety twig cutting seedlings when the rooting number of a single branch reaches 5-10 and the rooting length reaches more than 3 cm.
Compared with the prior art, the invention has the beneficial effects that:
the plant growth regulator provided by the invention is mainly obtained by compounding naphthylacetic acid, indolebutyric acid, gibberellin and 6-benzylaminopurine according to a specific proportion, achieves the technical effect of synergistic interaction through the synergistic interaction of the raw materials, and can greatly improve the survival rate of the cutting of the improved variety twig of eucommia ulmoides as proved by the experiment.
The preparation method of the plant growth regulator provided by the invention comprises the step of uniformly mixing the raw materials according to the formula amount to prepare the plant growth regulator. The preparation method has the advantages of simple preparation process and easy operation.
The plant growth regulator provided by the invention can be widely applied to cutting seedling of eucommia ulmoides fine variety twigs.
The invention provides a cutting seedling method for an improved variety twig of eucommia, which comprises the following steps: firstly, providing an aqueous solution with solute of the plant growth regulator as a cuttage solution; then soaking the eucommia ulmoides twig cutting slips in a cutting solution to obtain soaked eucommia ulmoides twig cutting slips; and then inserting the soaked eucommia ulmoides twig cutting slips into a seedling raising bed for seedling raising culture to obtain the eucommia ulmoides fine variety twig cutting seedlings. Experiments prove that the eucommia ulmoides fine variety twig cutting seedling method can greatly improve the eucommia ulmoides fine variety twig cutting survival rate, provides a fine variety twig cutting breeding technology for the development of the eucommia ulmoides industry, breaks through a single fine variety grafting breeding method adopted in the existing eucommia ulmoides production, enriches the eucommia ulmoides fine variety vegetative propagation method, accelerates the propagation speed of the eucommia ulmoides fine variety seedlings, overcomes the defects that in the breeding process of the fine variety grafted seedlings, grafted seedlings are not easy to identify due to grafting survival rate and the like, and the purity of the seedlings cannot be guaranteed, greatly relieves the urgent demand of the existing eucommia ulmoides production on the eucommia ulmoides fine variety cutting seedlings, remarkably improves the fine variety level of China, and promotes the rapid popularization of high-yield rubber fine varieties of eucommia ulmoides and the continuous and stable development of the eucommia ulmoides industry.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
According to one aspect of the invention, the plant growth regulator comprises the following components in percentage by mass:
15-35% of naphthylacetic acid, 35-55% of indolebutyric acid, 5-15% of gibberellin and 10-15% of 6-benzylaminopurine;
the sum of the mass percentages of the components in the plant growth regulator is 100%.
The plant growth regulator provided by the invention is mainly obtained by compounding naphthylacetic acid, indolebutyric acid, gibberellin and 6-benzylaminopurine according to a specific proportion, achieves the technical effect of synergistic interaction, and can greatly improve the survival rate of fine variety twig cuttage of eucommia through verification.
Typical but non-limiting preferred embodiments of the above-mentioned naphthylacetic acid are: 15%, 20%, 25%, 30% and 35%; typical but non-limiting preferred embodiments of the aforementioned indolebutyric acid are: 35%, 40%, 45%, 55% and 55%; typical but non-limiting preferred embodiments of the above gibberellins are: 5%, 8%, 10%, 12% and 15%; typical but non-limiting preferred embodiments of the above 6-benzylaminopurine are: 10%, 11%, 12%, 13%, 14% and 15%.
In a preferred embodiment of the present invention, the plant growth regulator comprises the following components in percentage by mass:
15-25% of naphthylacetic acid, 35-50% of indolebutyric acid, 5-12% of gibberellin and 10-13% of 6-benzylaminopurine;
the sum of the mass percentages of the components in the plant growth regulator is 100 percent;
preferably, the plant growth regulator comprises the following components in percentage by mass: 25% of naphthylacetic acid, 50% of indolebutyric acid, 12% of gibberellin and 13% of 6-benzylaminopurine.
In the invention, the technical effect of the plant growth regulator is further optimized by further adjusting and optimizing the dosage proportion of the raw materials of each component.
According to an aspect of the present invention, a method for preparing the above plant growth regulator, the method comprising the steps of:
the plant growth regulator is prepared by uniformly mixing the raw materials according to the formula ratio.
The preparation method of the plant growth regulator provided by the invention comprises the step of uniformly mixing the raw materials according to the formula amount to prepare the plant growth regulator. The preparation method has the advantages of simple preparation process and easy operation.
According to one aspect of the invention, the plant growth regulator is applied to cutting seedling raising of improved variety twigs of eucommia ulmoides.
The plant growth regulator provided by the invention can be widely applied to cutting seedling of eucommia ulmoides fine variety twigs.
Note: the eucommia ulmoides of the invention is typically but not limited to: "hua sec No. 1 '," hua sec No. 2', "hua sec No. 3 '," hua sec No. 4', "huasec No. 5 '," huasec No. 6', "huasec No. 7 '," huasec No. 8', "huasec No. 9 '," huasec No. 10', "huasec No. 11 '," huasec No. 12', "huasec No. 13 '," huasec No. 14', "huasec No. 16 '," huasec No. 17', "huasec No. 18 '," huasec No. 19', "huasec No. 20 '," huasec No. 21', "huasec No. 22 '," huasec No. 23', "huasec No. 24 '," huasec No. 25', "huasec No. 26 '," huasec No. 27', "huasec No. 28 'and" huasec No. 30', the specific examination is as shown in the following table:
according to one aspect of the invention, the cutting seedling method of the improved variety of eucommia ulmoides shoots comprises the following steps:
providing an aqueous solution with a solute of the plant growth regulator as a cutting solution; then soaking the eucommia ulmoides twig cutting slips in a cutting solution to obtain soaked eucommia ulmoides twig cutting slips; and then inserting the soaked eucommia ulmoides twig cutting slips into a seedling raising bed for seedling raising culture to obtain the eucommia ulmoides fine variety twig cutting seedlings.
The invention provides a cutting seedling method for an improved variety twig of eucommia, which comprises the following steps: firstly, providing an aqueous solution with solute of the plant growth regulator as a cuttage solution; then soaking the eucommia ulmoides twig cutting slips in a cutting solution to obtain soaked eucommia ulmoides twig cutting slips; and then inserting the soaked eucommia ulmoides twig cutting slips into a seedling raising bed for seedling raising culture to obtain the eucommia ulmoides fine variety twig cutting seedlings. Experiments prove that the eucommia ulmoides fine variety twig cutting seedling method can greatly improve the eucommia ulmoides fine variety twig cutting survival rate, provides a fine variety twig cutting breeding technology for the development of the eucommia ulmoides industry, breaks through a single fine variety grafting breeding method adopted in the existing eucommia ulmoides production, enriches the eucommia ulmoides fine variety vegetative propagation method, accelerates the propagation speed of the eucommia ulmoides fine variety seedlings, overcomes the defects that in the breeding process of the fine variety grafted seedlings, grafted seedlings are not easy to identify due to grafting survival rate and the like, and the purity of the seedlings cannot be guaranteed, greatly relieves the urgent demand of the existing eucommia ulmoides production on the eucommia ulmoides fine variety cutting seedlings, remarkably improves the fine variety level of China, and promotes the rapid popularization of high-yield rubber fine varieties of eucommia ulmoides and the continuous and stable development of the eucommia ulmoides industry.
In a preferred embodiment of the present invention, the method for preparing the cutting solution comprises the following steps:
dissolving a plant growth regulator in a solvent to obtain a dissolved solution A; adding water into the dissolving solution A to dilute the dissolving solution A into a cutting solution of 200-1000 mg/kg;
preferably, the solvent is ethanol, preferably ethanol with a concentration of 75%;
in a preferred embodiment, in order to improve the solubility of the plant growth regulator in the aqueous solution, the plant growth regulator is first dissolved in an ethanol solution and then diluted with water to obtain a cutting solution.
In the above preferred embodiment, the solid-to-liquid ratio of the plant growth regulator to ethanol in the solution a is 1: 15-25 g/ml;
in a preferred embodiment, the solution A contains the plant growth regulator and ethanol at a solid-to-liquid ratio of 1:20 g/ml.
In the preferable embodiment, the concentration of the cutting solution is 200-1000 mg/kg.
In a preferred embodiment, the concentration of the cutting solution is 800 mg/kg.
Typical but non-limiting preferred embodiments of the concentration of the above-mentioned cutting solution are: 200mg/kg, 400mg/kg, 600mg/kg, 800mg/kg and 1000 mg/kg.
In a preferred embodiment of the invention, the soaking time for soaking the eucommia ulmoides tender branch cutting slips in the cutting solution is 5-120 seconds.
In a preferred embodiment, the soaking time is 25 seconds.
In a preferred embodiment of the invention, the seedling bed is mainly prepared from peatmoss and perlite;
preferably, the mass ratio of the peatmoss to the perlite is 2-4: 1, preferably 3: 1;
preferably, the thickness of the seedling raising bed is 15-20 cm.
In a preferred embodiment of the invention, the temperature of the seedling culture is 22-30 ℃, and the relative humidity of air is 80-98%.
In a preferred embodiment, the temperature of the seedling culture is 25 ℃ and the relative humidity of air is 95%.
In a preferred embodiment of the present invention, the method for raising eucommia ulmoides fine variety shoots by cutting comprises the following steps:
(a) and preparing a cutting solution: mixing plant growth regulator in a ratio of 1: dissolving 15-25 g/ml of solid-to-liquid ratio in ethanol to obtain a solution A; adding water into the dissolving solution A to dilute the dissolving solution A into a cutting solution of 200-1000 mg/kg;
(b) providing semi-lignified eucommia ulmoides twigs with the length of 8-16 cm and the base stem of 0.2-0.6 cm as eucommia ulmoides twigs cutting slips, and soaking the eucommia ulmoides twigs cutting slips in the cutting solution obtained in the step (a) for 5-120 seconds to obtain soaked eucommia ulmoides twigs cutting slips;
(c) mixing turfy soil and perlite according to the weight ratio of 3:1, uniformly mixing the materials in a mass ratio to prepare a seedling bed with the thickness of 15-20 cm, inserting the eucommia ulmoides twig cutting slips obtained in the step (b) into the seedling bed, performing seedling culture in an environment with the temperature of 22-30 ℃ and the relative air humidity of 80-98%, and obtaining the eucommia ulmoides fine variety twig cutting seedlings when the rooting number of a single branch reaches 5-10 and the rooting length reaches more than 3 cm.
As a preferred embodiment, the selection of the variety, the concentration and the cutting processing time of the plant growth regulator, the selection of the cutting screening, the cutting substrate optimization, the control of the temperature and the humidity of the cutting environment and other culture parameters break through the technical problem of the twig cutting of the eucommia ulmoides fine variety big tree, and the survival rate of the twig cutting is greatly improved. Experiments prove that the cuttage survival rate can be improved to more than 90% from the existing 5%.
Preferably, the eucommia ulmoides improved variety twig cutting seedling method comprises the following steps:
(1) optimizing the formula of the plant growth regulator: the optimized formula of the plant growth regulator for the twig cuttage of the improved variety of eucommia ulmoides comprises the following steps: mixing the plant growth regulator evenly according to the mass percentage of 15-35 percent of naphthylacetic acid (NAA), 35-55 percent of indolebutyric acid (IBA), 5-15 percent of Gibberellin (GA) and 10-15 percent of cytokinin (6-BA);
(2) and preparing a cutting solution: fully dissolving the plant growth regulator with the optimized formula in the step (1) by using 75% alcohol, and then adding purified water to prepare an aqueous solution with the concentration of 200-1000 mg/kg for later use;
(3) and optimizing the cutting environment and the substrate: cutting in an intelligent greenhouse, uniformly mixing turfy soil and perlite according to the volume ratio of 3:1 to prepare a cutting medium, paving the cutting medium into a cutting bed with the thickness of 15-20 cm, and then disinfecting by using 0.3% potassium permanganate;
(4) and cutting and screening: selecting semi-lignified twigs of a large variety of eucommia ulmoides such as 'Huazhong No. 6' and the like as cutting slips, wherein the length of each cutting slip is 8-16 cm, and the base stem of each cutting slip is 0.2-0.6 cm;
(5) and cutting the rice shoot: reserving 2-3 tender leaves according to the cutting slips collected in the step (4), and soaking in the cutting solution prepared in the step (2) for 5-120 seconds, wherein the cutting slip soaking depth is 3-5 cm;
(6) and cutting: cutting the eucommia ulmoides fine variety twig cuttings soaked in the cutting solution on the cutting bed manufactured in the step (3) to a cutting depth of 5-10 cm;
(7) and regulating and controlling the environmental temperature and humidity after cuttage: after cutting, keeping the relative humidity of air at 80% -98% and the temperature at 22-30 ℃;
(8) and transplanting cutting seedlings: and when the rooting quantity of a single branch reaches 5-10 and the rooting length reaches more than 3cm, transplanting the single branch in a field, and keeping the air humidity of 80-95% within one week after transplanting.
The technical solution of the present invention will be further described with reference to examples and comparative examples.
Example 1
A method for cutting and breeding improved variety of eucommia ulmoides through shoots comprises the following steps:
(1) and the formula of the plant growth regulator comprises the following components: mixing plant growth regulator with Naphthalene Acetic Acid (NAA)15 wt%, indolebutyric acid (IBA)55 wt%, Gibberellin (GA)15 wt%, and cytokinin (6-BA)15 wt%;
(2) and preparing a cutting solution: fully dissolving the plant growth regulator with the optimized formula in the step (1) by using 75% alcohol, and then adding purified water to prepare an aqueous solution with the concentration of 200mg/kg to obtain a cuttage solution;
(3) cutting environment and substrate: cutting in an intelligent greenhouse, uniformly mixing turfy soil and perlite according to the volume ratio of 3:1 to prepare a cutting medium, paving the cutting medium into a cutting bed with the thickness of 15cm, and then disinfecting by using 0.3% potassium permanganate;
(4) and cutting and screening: selecting semi-lignified twigs of a large variety of eucommia ulmoides such as 'Huazhong No. 6' and the like as cutting slips, wherein the length of each cutting slip is 8-16 cm, and the base stem of each cutting slip is 0.2-0.6 cm;
(5) and cutting the rice shoot: reserving 2-3 tender leaves according to the cutting slips collected in the step (4), and soaking in the cutting solution prepared in the step (2) for 120 seconds, wherein the cutting slip soaking depth is 3-5 cm;
(6) and cutting: cutting the eucommia ulmoides fine variety twig cuttings soaked in the cutting solution on the cutting bed manufactured in the step (3) to a cutting depth of 5-10 cm;
(7) and regulating and controlling the environmental temperature and humidity after cuttage: after cuttage, keeping the relative humidity of air at 80% -85% and the temperature at 22-25 ℃;
(8) and transplanting cutting seedlings: and when the rooting quantity of a single branch reaches 5-10 and the rooting length reaches more than 3cm, transplanting the single branch in a field, and keeping the air humidity of 80-95% within one week after transplanting.
Example 2
A method for cutting and breeding improved variety of eucommia ulmoides through shoots comprises the following steps:
(1) and the formula of the plant growth regulator comprises the following components: the optimized formula of the plant growth regulator for the twig cuttage of the improved variety of eucommia ulmoides comprises the following steps: mixing plant growth regulator with naphthylacetic acid (NAA)35 wt%, indolebutyric acid (IBA)35 wt%, Gibberellin (GA)15 wt%, and cytokinin (6-BA)15 wt%;
(2) and preparing a cutting solution: fully dissolving the plant growth regulator with the optimized formula in the step (1) by using 75% alcohol, and then adding purified water to prepare an aqueous solution with the concentration of 1000mg/kg to obtain a cuttage solution;
(3) cutting environment and substrate: cutting in an intelligent greenhouse, uniformly mixing turfy soil and perlite according to the volume ratio of 3:1 to prepare a cutting medium, paving the cutting medium into a cutting bed with the thickness of 20cm, and then disinfecting by using 0.3% potassium permanganate;
(4) and cutting and screening: selecting semi-lignified twigs of a large variety of eucommia ulmoides such as 'Huazhong No. 6' and the like as cutting slips, wherein the length of each cutting slip is 8-16 cm, and the base stem of each cutting slip is 0.2-0.6 cm;
(5) and cutting the rice shoot: reserving 2-3 tender leaves according to the cutting slips collected in the step (4), and soaking in the cutting solution prepared in the step (2) for 5 seconds, wherein the cutting slip soaking depth is 3-5 cm;
(6) and cutting: cutting the eucommia ulmoides fine variety twig cuttings soaked in the cutting solution on the cutting bed manufactured in the step (3) to a cutting depth of 5-10 cm;
(7) and environment temperature and humidity after cuttage: after cutting, keeping the relative humidity of air at 80% -98% and the temperature at 22-30 ℃;
(8) and transplanting cutting seedlings: and when the rooting quantity of a single branch reaches 5-10 and the rooting length reaches more than 3cm, transplanting the single branch in a field, and keeping the air humidity of 80-95% within one week after transplanting.
Example 3
A method for cutting and breeding improved variety of eucommia ulmoides through shoots comprises the following steps:
(1) and the formula of the plant growth regulator comprises the following components: mixing plant growth regulator with Naphthalene Acetic Acid (NAA)25 wt%, indolebutyric acid (IBA)50 wt%, Gibberellin (GA)12 wt%, and cytokinin (6-BA)13 wt%;
(2) and preparing a cutting solution: fully dissolving the plant growth regulator with the optimized formula in the step (1) by using 75% alcohol, and then adding purified water to prepare an aqueous solution with the concentration of 500mg/kg to obtain a cuttage solution;
(3) cutting environment and substrate: cutting in an intelligent greenhouse, uniformly mixing turfy soil and perlite according to the volume ratio of 3:1 to prepare a cutting medium, paving the cutting medium into a cutting bed with the thickness of 15-20 cm, and then disinfecting by using 0.3% potassium permanganate;
(4) and cutting and screening: selecting semi-lignified twigs of a large variety of eucommia ulmoides such as 'Huazhong No. 6' and the like as cutting slips, wherein the length of each cutting slip is 8-16 cm, and the base stem of each cutting slip is 0.2-0.6 cm;
(5) and cutting the rice shoot: reserving 2-3 tender leaves according to the cutting slips collected in the step (4), and soaking in the cutting solution prepared in the step (2) for 50 seconds, wherein the cutting slip soaking depth is 3-5 cm;
(6) and cutting: cutting the eucommia ulmoides fine variety twig cuttings soaked in the cutting solution on the cutting bed manufactured in the step (3) to a cutting depth of 5-10 cm;
(7) and environment temperature and humidity after cuttage: after cutting, keeping the relative humidity of air at 80% -98% and the temperature at 22-30 ℃;
(8) and transplanting cutting seedlings: and when the rooting quantity of a single branch reaches 5-10 and the rooting length reaches more than 3cm, transplanting the single branch in a field, and keeping the air humidity of 80-95% within one week after transplanting.
Example 4
This example is the same as example 3 except that the concentration of the cutting solution in step (2) is 800 mg/kg.
Example 5
This example was the same as example 3 except that the immersion concentration in (5) was 20 seconds.
Comparative example 1
This comparative example is the same as example 3 except that the composition of the plant growth regulator is different from example 3;
the plant growth regulator comprises the following components in percentage by mass:
100% naphthylacetic acid;
the concentration of the cutting solution is 300mg/kg, the cutting solution is soaked for 2 hours, and the survival rate of 'Huazhong No. 8' reaches 91.2 percent.
Experimental example 1
In order to show that the eucommia ulmoides improved variety twig cutting seedling method has the technical effect of greatly improving the eucommia ulmoides improved variety twig cutting survival rate, the eucommia ulmoides improved variety twig cutting breeding methods of examples 1-5 and comparative example 1 are adopted to carry out seedling experiments at present, the seedling experiments are divided into 6 groups, 100 eucommia ulmoides semi-lignified twigs with the length of 8-16 cm and the base stem of 0.2-0.6 cm are selected from each group and used as eucommia ulmoides twig cuttings to carry out cutting seedling, and the specific results are as follows:
from the above table, the survival rate of the cutting seedling of the improved variety of eucommia ulmoides by using the technical scheme of the invention can reach more than 90%, wherein the survival rate is the highest in the example 3. When the concentration of the rooting agent is higher, the soaking time is reduced, otherwise, the cuttings are damaged, and the survival rate is influenced.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. The plant growth regulator is characterized by comprising the following components in percentage by mass:
15-35% of naphthylacetic acid, 35-55% of indolebutyric acid, 5-15% of gibberellin and 10-15% of 6-benzylaminopurine;
the sum of the mass percentages of the components in the plant growth regulator is 100%.
2. The plant growth regulator according to claim 1, comprising the following components in percentage by mass:
15-25% of naphthylacetic acid, 35-50% of indolebutyric acid, 5-12% of gibberellin and 10-13% of 6-benzylaminopurine;
the sum of the mass percentages of the components in the plant growth regulator is 100 percent;
preferably, the plant growth regulator comprises the following components in percentage by mass: 25% of naphthylacetic acid, 50% of indolebutyric acid, 12% of gibberellin and 13% of 6-benzylaminopurine.
3. A method for preparing a plant growth regulator according to claim 1 or 2, wherein the preparation method comprises the steps of:
the plant growth regulator is prepared by uniformly mixing the raw materials according to the formula ratio.
4. Use of the plant growth regulator according to claim 1 or 2 in cutting seedling of an improved variety of eucommia ulmoides.
5. A cutting seedling method for improved variety twigs of eucommia ulmoides is characterized by comprising the following steps:
providing an aqueous solution with a solute of the plant growth regulator of claim 1 or 2 as a cutting solution; then soaking the eucommia ulmoides twig cutting slips in a cutting solution to obtain soaked eucommia ulmoides twig cutting slips; and then inserting the soaked eucommia ulmoides twig cutting slips into a seedling raising bed for seedling raising culture to obtain the eucommia ulmoides fine variety twig cutting seedlings.
6. The eucommia ulmoides improved variety twig cutting seedling method according to claim 5, wherein the preparation method of the cutting solution comprises the following steps:
dissolving a plant growth regulator in a solvent to obtain a dissolved solution A; adding water into the dissolving solution A to dilute the dissolving solution A into a cutting solution of 200-1000 mg/kg;
preferably, the solvent is ethanol, preferably ethanol with a concentration of 75%;
more preferably, the solid-to-liquid ratio of the plant growth regulator to ethanol in the solution a is 1: 15-25 g/ml;
preferably, the concentration of the cutting solution is 200-1000 mg/kg.
7. The eucommia ulmoides improved variety twig cutting seedling raising method according to claim 5, wherein the soaking time for soaking the eucommia ulmoides twig cutting shoot in the cutting solution is 5-120 seconds.
8. The eucommia ulmoides improved variety twig cutting seedling method according to claim 5, wherein the seedling bed is mainly prepared from turfy soil and perlite;
preferably, the mass ratio of the peatmoss to the perlite is 2-4: 1, preferably 3: 1;
preferably, the thickness of the seedling raising bed is 15-20 cm.
9. An improved eucommia ulmoides seed twig cutting seedling method according to claim 5, wherein the temperature of seedling culture is 22-30 ℃, and the relative air humidity is 80-98%.
10. The eucommia ulmoides improved variety twig cutting seedling method according to claim 5, wherein the eucommia ulmoides improved variety twig cutting seedling method comprises the following steps:
(a) and preparing a cutting solution: mixing plant growth regulator in a ratio of 1: dissolving 15-25 g/ml of solid-to-liquid ratio in ethanol to obtain a solution A; adding water into the dissolving solution A to dilute the dissolving solution A into a cutting solution of 200-1000 mg/kg;
(b) providing semi-lignified eucommia ulmoides twigs with the length of 8-16 cm and the base stem of 0.2-0.6 cm as eucommia ulmoides twigs cutting slips, and soaking the eucommia ulmoides twigs cutting slips in the cutting solution obtained in the step (a) for 5-120 seconds to obtain soaked eucommia ulmoides twigs cutting slips;
(c) mixing turfy soil and perlite according to the weight ratio of 3:1, uniformly mixing the materials in a mass ratio to prepare a seedling bed with the thickness of 15-20 cm, inserting the eucommia ulmoides twig cutting slips obtained in the step (b) into the seedling bed, and performing seedling culture for 45-60 days in an environment with the temperature of 22-30 ℃ and the air relative humidity of 80-98%, wherein when the rooting number of a single branch reaches 5-10 and the rooting length reaches 3-4 cm, an improved eucommia ulmoides twig cutting seedling is obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011236585.9A CN112273383A (en) | 2020-11-06 | 2020-11-06 | Plant growth regulator, preparation method and eucommia improved variety twig cutting seedling method using plant growth regulator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011236585.9A CN112273383A (en) | 2020-11-06 | 2020-11-06 | Plant growth regulator, preparation method and eucommia improved variety twig cutting seedling method using plant growth regulator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112273383A true CN112273383A (en) | 2021-01-29 |
Family
ID=74351827
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011236585.9A Pending CN112273383A (en) | 2020-11-06 | 2020-11-06 | Plant growth regulator, preparation method and eucommia improved variety twig cutting seedling method using plant growth regulator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112273383A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113080064A (en) * | 2021-05-18 | 2021-07-09 | 中国科学院合肥物质科学研究院 | Low-cost rapid micro-propagation method for excellent eucommia ulmoides seedlings |
| CN114731857A (en) * | 2022-02-28 | 2022-07-12 | 江西普正制药股份有限公司 | Cutting propagation method of eucommia ulmoides |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101491215A (en) * | 2009-02-19 | 2009-07-29 | 周玉玲 | Chinese toon tissue-culture quick propagation technique and culture medium proportion |
| CN104686183A (en) * | 2015-04-02 | 2015-06-10 | 戈静 | Efficient elderberry cultivation method |
| CN107347647A (en) * | 2017-08-04 | 2017-11-17 | 南京江宁台湾农民创业园发展有限公司 | One kind promotes purple perilla root media |
| CN108124768A (en) * | 2017-12-14 | 2018-06-08 | 浙江诚邦园林股份有限公司 | A kind of method suitable for color leafed plants tissue culture seedling rooting |
| CN108812274A (en) * | 2018-04-12 | 2018-11-16 | 日照市茶叶科学研究所 | Tea cutting breeds seedbed and tea tree spray non-woven fabrics Light media aerosol fast breeding technique |
-
2020
- 2020-11-06 CN CN202011236585.9A patent/CN112273383A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101491215A (en) * | 2009-02-19 | 2009-07-29 | 周玉玲 | Chinese toon tissue-culture quick propagation technique and culture medium proportion |
| CN104686183A (en) * | 2015-04-02 | 2015-06-10 | 戈静 | Efficient elderberry cultivation method |
| CN107347647A (en) * | 2017-08-04 | 2017-11-17 | 南京江宁台湾农民创业园发展有限公司 | One kind promotes purple perilla root media |
| CN108124768A (en) * | 2017-12-14 | 2018-06-08 | 浙江诚邦园林股份有限公司 | A kind of method suitable for color leafed plants tissue culture seedling rooting |
| CN108812274A (en) * | 2018-04-12 | 2018-11-16 | 日照市茶叶科学研究所 | Tea cutting breeds seedbed and tea tree spray non-woven fabrics Light media aerosol fast breeding technique |
Non-Patent Citations (3)
| Title |
|---|
| 柏劲松等: "杜仲嫩枝扦插试验", 《中国农村小康科技》 * |
| 邵应韶等: "《时花栽培》", 31 May 1982 * |
| 马小军等: "全光喷雾和生长调节剂对杜仲插穗的影响", 《中国医学科学院学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113080064A (en) * | 2021-05-18 | 2021-07-09 | 中国科学院合肥物质科学研究院 | Low-cost rapid micro-propagation method for excellent eucommia ulmoides seedlings |
| CN113080064B (en) * | 2021-05-18 | 2022-07-29 | 中国科学院合肥物质科学研究院 | Low-cost rapid micro-propagation method for excellent eucommia ulmoides seedlings |
| CN114731857A (en) * | 2022-02-28 | 2022-07-12 | 江西普正制药股份有限公司 | Cutting propagation method of eucommia ulmoides |
| CN114731857B (en) * | 2022-02-28 | 2023-10-27 | 江西普正制药股份有限公司 | Cutting propagation method of eucommia ulmoides |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103299812B (en) | Apocarya hardwood cutting seedling raising method | |
| CN103238492B (en) | Process for facilitating sprouting of adventitious roots of apocarya branches | |
| CN112273383A (en) | Plant growth regulator, preparation method and eucommia improved variety twig cutting seedling method using plant growth regulator | |
| CN107371960A (en) | A kind of implantation methods of fertile mandarin orange | |
| CN102612961A (en) | Method for cultivating olive seedlings | |
| CN107549017A (en) | A kind of morningstar lily lily propagation method | |
| CN114097613A (en) | Method for establishing paper mulberry leaf tissue culture regeneration system | |
| CN108739380B (en) | Method for one-time seedling formation of rhizoma bletillae tissue culture seedlings | |
| CN106717792A (en) | A kind of implantation methods of high-quality red heart dragon fruit | |
| CN101336597A (en) | Scaly holly root propagation method | |
| CN102599001B (en) | Method for raising olive seedlings in hotbed | |
| CN110832997A (en) | Breeding method for hardwood cutting of cudrania tricuspidata | |
| CN108887110B (en) | Planting method of gynura divaricata | |
| KR101176518B1 (en) | Development of Cudrania tricuspidata | |
| CN111670702B (en) | Myrtle hardwood cutting method | |
| CN110583286B (en) | Method for improving sprouting capacity of eucalyptus globulus seedlings based on exogenous hormones and stumping height | |
| CN107360829A (en) | The cuttage breeding method of Nandina domestica'Fire power' | |
| KR101034047B1 (en) | Method to manufacture plant growth regulator using peat moss | |
| CN111149526A (en) | Method for improving cuttage rooting of Chinese roses | |
| CN112841032B (en) | Infinite bud multiplication medium for mallotus maritima and method for in-vitro rapid propagation of mallotus maritima | |
| CN110558071A (en) | Cutting seedling method for olive twigs | |
| CN110089388A (en) | A kind of Rhizoma Gastrodiae soilless culture substrate | |
| CN110612989B (en) | Synergistic naphthylacetic acid soluble liquor | |
| Boboc et al. | Studies concerning in vivo and in vitro vegetative propagation of Passiflora quadrangularis species. | |
| KR102352376B1 (en) | Cultivation Method of Vernonia amygdalina Containing High Polyphenols |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210129 |
|
| RJ01 | Rejection of invention patent application after publication |