CN114410425A - Photosynthetic microorganism culture apparatus - Google Patents

Photosynthetic microorganism culture apparatus Download PDF

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Publication number
CN114410425A
CN114410425A CN202210034684.1A CN202210034684A CN114410425A CN 114410425 A CN114410425 A CN 114410425A CN 202210034684 A CN202210034684 A CN 202210034684A CN 114410425 A CN114410425 A CN 114410425A
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air
light
mechanism groove
cylinder
transparent
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CN114410425B (en
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冯鸿彬
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Shanghai Zhichu Instrument Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/22Transparent or translucent parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • C12M27/04Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a photosynthetic microorganism culture device which comprises a culture pond, wherein a transparent partition plate is arranged on the culture pond, a plurality of air holes are formed in the transparent partition plate, a light-facing cylinder is embedded in the transparent partition plate, a mechanism groove is formed in the side wall of the culture pond, the bottom in the mechanism groove is communicated with an air inlet pipe, an air outlet pipe communicated with the inside of the mechanism groove is further arranged on the inner wall of the culture pond, a one-way valve is arranged at the air outlet end of the air outlet pipe, a rotating wheel is rotatably connected to the bottom in the mechanism groove, and a plurality of centrifugal blades are fixedly connected to the side wall of the rotating wheel. The driving device can be operated by light energy, and the centrifugal blades are continuously rotated by the matching of the one-way bearing and the rotating wheel, so that air is blown into the lower part of algae liquid, wherein most of gases such as nitrogen, oxygen and the like in the air can be quickly discharged when being insoluble in water, and carbon dioxide is soluble in water and can be absorbed by microalgae in the algae liquid in the rising process, thereby promoting the photosynthesis of the microalgae and improving the cultivation efficiency.

Description

Photosynthetic microorganism culture apparatus
Technical Field
The invention relates to the technical field of microorganism culture equipment, in particular to a photosynthetic microorganism culture device.
Background
Photosynthetic microorganisms or plant cells, capable of synthesizing water and inorganic minerals into organic substances using light energy. Microalgae are a very representative photosynthetic microorganism, and the commercial scale culture of microalgae is mainly carried out in an open culture pond at present.
And because the illumination is difficult to be intensively irradiated below the algae liquid in the pool, the algae liquid in the pool is not sufficiently illuminated, and the utilization of the light energy is limited. The application number is "201110444854.5" and has proposed a "spectral reactor, photosynthetic microorganism culture apparatus", this scheme is mainly through setting up the holder that has transparent barrel, make illumination can shine the algae liquid below through transparent barrel, increase the illumination area, but set up the flat-plate holder of algae liquid level top, transparent barrel also can hinder algae liquid and air interaction, on the one hand, the carbon dioxide in having hindered little algae absorption air and carried out photosynthesis, on the other hand, the radiating effect in culture pond has also been reduced, easily make the culture pond heat up and cause photosynthetic microorganism to die. In view of this, the present document proposes a photosynthetic microorganism culture apparatus.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a photosynthetic microorganism culture device.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a photosynthetic microorganism culture apparatus, includes cultivates the pond, be equipped with transparent baffle on the cultivation pond, be equipped with a plurality of bleeder vents on the transparent baffle, transparent baffle inlays and is equipped with a light facing section of thick bamboo, the lateral wall of cultivation pond is seted up there is the mechanism groove, mechanism inslot bottom intercommunication has the intake pipe, still be equipped with on the cultivation pond inner wall with the communicating outlet duct of mechanism inslot portion, just the end of giving vent to anger of outlet duct is equipped with the check valve, mechanism inslot bottom rotates and is connected with the runner, just a plurality of centrifugal blades of lateral wall fixedly connected with of runner, the runner is connected with a screw thread section of thick bamboo through one-way bearing, screw thread section of thick bamboo female connection has the screw rod, the mechanism inslot is installed the drive arrangement that drive screw rod reciprocated.
Preferably, the driving device comprises a transparent cylinder fixedly arranged at the top in the mechanism groove, the transparent cylinder is provided with a mixture of silver bromide and copper oxide, the inner wall of the mechanism groove is provided with a light hole, a glass plate is embedded in the light hole, the side wall of the threaded cylinder is fixedly connected with a light screen through a plurality of connecting rods, the transparent cylinder is internally connected with a piston in a sealing and sliding manner, the lower end of the piston is fixedly connected with an ejector rod, and the ejector rod is fixedly connected with a screw rod.
Preferably, the ejector rod penetrates through the transparent cylinder in a sealing mode, the transparent cylinder is communicated with an exhaust pipe and an exhaust pipe, one-way valves are installed in the exhaust pipe and the exhaust pipe, and the air outlet end of the exhaust pipe is arranged above the liquid level of the culture pond.
Preferably, the light shielding plate is an arc-shaped light-tight plate, and the forward projection area of the light shielding plate is larger than the area of the glass plate.
Preferably, one end of the connecting rod is fixedly connected to the side wall of the light screen, and the other end of the connecting rod is fixedly connected to the side wall of the threaded cylinder.
The invention has the following beneficial effects:
1. by arranging the driving device, the one-way bearing, the rotating wheel and the centrifugal blade, the driving device can be operated by light energy, and the centrifugal blade can continuously rotate by matching the one-way bearing and the rotating wheel, so that air is blown into the lower part of the algae liquid, wherein most of nitrogen, oxygen and other gases in the air are insoluble in water and can be quickly discharged, and carbon dioxide is soluble in water and can be absorbed by microalgae in the algae liquid in the rising process to promote photosynthesis of the microalgae and improve cultivation efficiency;
2. through setting up exhaust tube and blast pipe, can make drive arrangement operation make the piston reciprocate simultaneously at light energy to under the one-way current limiting effect of check valve in exhaust tube and blast pipe, greatly increased algae liquid level top air flow velocity makes the heat that algae liquid produced can distribute away fast, avoids algae liquid to produce high temperature and causes the microorganism dead.
Drawings
FIG. 1 is a schematic structural view of a photosynthetic microorganism culture apparatus according to the present invention;
FIG. 2 is an enlarged view of the structure at A in FIG. 1;
FIG. 3 is a schematic view of the connection structure of the threaded cylinder, the connecting rod and the light shielding plate according to the present invention.
In the figure: 1 culture pond, 2 transparent clapboards, 3 air holes, 4 light-facing cylinders, 5 light holes, 6 transparent cylinders, 7 pistons, 8 mechanism grooves, 9 rotating wheels, 10 centrifugal blades, 11 ejector rods, 12 screw rods, 13 threaded cylinders, 14 glass plates, 15 one-way bearings, 16 shading plates, 17 air inlet pipes, 18 air outlet pipes, 19 air outlet pipes and 20 air outlet pipes.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-3, a photosynthetic microorganism culture apparatus comprises a culture pond 1, a transparent partition plate 2 is arranged on the culture pond 1, a plurality of air vents 3 are arranged on the transparent partition plate 2, a light facing cylinder 4 is embedded in the transparent partition plate 2, a mechanism groove 8 is arranged on the side wall of the culture pond 1, an air inlet pipe 17 is communicated with the bottom in the mechanism groove 8, an air outlet pipe 18 communicated with the interior of the mechanism groove 8 is also arranged on the inner wall of the culture pond 1, and the air outlet end of the air outlet pipe 18 is provided with a one-way valve, the bottom in the mechanism groove 8 is rotationally connected with a rotating wheel 9, the side wall of the runner 9 is fixedly connected with a plurality of centrifugal blades 10, the runner 9 is connected with a thread cylinder 13 through a one-way bearing 15, it should be noted that through the arrangement of the one-way bearing 15, when the threaded cylinder 13 rotates in the forward direction, the runner 9 can be driven to rotate by the one-way bearing 15, and when the threaded cylinder 13 rotates in the reverse direction, the runner 9 cannot be driven to rotate by the one-way bearing 15. The rotating wheel 9 can only continue to rotate in one direction when the screw cylinder 13 rotates back and forth.
A screw 12 is connected with the thread cylinder 13 through internal threads, and a driving device for driving the screw 12 to move up and down is installed in the mechanism groove 8.
Drive arrangement is including the fixed transparent section of thick bamboo 6 that sets up top in mechanism groove 8, transparent section of thick bamboo 6 is equipped with silver bromide and copper oxide mixture, light trap 5 has been seted up on mechanism groove 8's the inner wall, the embedded glass board 14 that is equipped with of light trap 5, the lateral wall of screw thread section of thick bamboo 13 is through a plurality of connecting rod fixedly connected with light screen 16, and is concrete, the one end fixed connection of connecting rod is on the lateral wall of light screen 16, the other end fixed connection of connecting rod is on the 13 lateral walls of screw thread section of thick bamboo, light screen 16 is the arc light-tight board, and the forward projection area of light screen 16 is greater than the area of glass board 14. This ensures that when the light shielding plate 16 rotates the position of the glass plate 14, the light rays incident on the glass plate 14 can be completely blocked to prevent the light rays from shining into the transparent cylinder 6.
The piston 7 is connected in the transparent cylinder 6 in a sealing and sliding manner, the lower end of the piston 7 is fixedly connected with a mandril 11, and the mandril 11 is fixedly connected with a screw 12. The push rod 11 penetrates through the transparent cylinder 6 in a sealing manner, and it should be noted that, as shown in fig. 2, when the screw 12 moves up to the highest position, the push rod 11 still partially exposes out of the transparent cylinder 6, so that the lower end of the transparent cylinder 6 can be kept in a sealing state when the push rod 11 moves up and down.
An air suction pipe 19 and an air exhaust pipe 20 are communicated with the transparent cylinder 6, check valves are respectively arranged in the air suction pipe 19 and the air exhaust pipe 20, and the air outlet end of the air exhaust pipe 20 is arranged above the liquid level of the culture pond 1. It should be noted that the check valve in the air suction pipe 19 limits air to enter the transparent cylinder 6 from the air suction pipe 19 only in one direction, and the check valve in the air exhaust pipe 20 limits air to flow from the transparent cylinder 6 to the air exhaust pipe 20 only in one direction.
This device is in the use, and the illumination can shine in 1 algae liquid level below of culture pond through meeting a light section of thick bamboo 4 for photosynthetic microorganism homoenergetic everywhere below algae liquid level receives the illumination. Meanwhile, light is irradiated into the transparent cylinder 6 through the glass plate 14, silver bromide in the transparent cylinder 6 is decomposed into silver and bromine after being irradiated by the light, and the generated bromine is in a gas state, so that the piston 7 can be pushed to move downwards by increasing the air pressure above the piston 7, and the screw rod 12 is pushed to move downwards by the ejector rod 11, so that the threaded cylinder 13 in threaded connection with the screw rod 12 rotates forwards.
The thread cylinder 13 is rotated to drive the light screen 16 to rotate through the connecting rod, the light screen 16 can be driven to rotate to the position of the glass plate 14 after the thread cylinder 13 is rotated, so that the light can be prevented from being irradiated into the mechanism groove 8, silver and bromine in the transparent cylinder 6 lose the light and then are changed back to silver bromide under the catalysis of copper oxide, therefore, the air pressure above the piston 7 is reduced, the atmospheric pressure pushes the piston 7 to move upwards, and simultaneously the ejector rod 11 and the screw rod 12 are pulled to move upwards to reset, so that the thread cylinder 13 and the light screen 16 reversely rotate to reset, and the light can be irradiated to the transparent cylinder 6 at the moment. Therefore, in the device, the light irradiates the transparent cylinder 6 periodically, so that the silver bromide in the transparent cylinder 6 is decomposed and synthesized repeatedly, the air pressure above the piston 7 is changed in a periodic increasing and decreasing mode, the threaded cylinder 13 rotates back and forth under the matching of the screw 12 and the threaded cylinder 13, the rotating wheel 9 and the centrifugal blades 10 on the side wall of the rotating wheel continuously rotate in a single direction under the action of the single-direction bearing 15, air can be continuously sucked in through the air inlet pipe 17 and discharged into the algae liquid in the culture pond 1 through the air outlet pipe 18, most of oxygen and nitrogen in the air are insoluble in water and directly changed into bubbles to escape, a small amount of carbon dioxide can be partially dissolved in the water and fully contacts with photosynthetic microorganisms in the algae liquid to promote photosynthesis of the photosynthetic microorganisms, and the culture efficiency of the photosynthetic microorganisms is improved.
In addition further, under the one-way current limiting effect of check valve in exhaust tube 19 and blast pipe 20, still can make the confined space below piston 7 increase when piston 7 moves up to produce the negative pressure and inhale through exhaust tube 19 with the air, then blow off the air by blast pipe 20 when piston 7 moves down, sustainable quickening the air flow of algae liquid level top like this, thereby will cultivate the heat that pond 1 produced in the process of cultivateing and distribute away fast, avoid cultivating pond 1 heat and pile up the production high temperature and cause photosynthetic microorganism to die.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (5)

1. A photosynthetic microorganism culture device comprises a culture pond (1), wherein a transparent partition plate (2) is arranged on the culture pond (1), a plurality of air holes (3) are formed in the transparent partition plate (2), a light facing cylinder (4) is embedded in the transparent partition plate (2), the photosynthetic microorganism culture device is characterized in that a mechanism groove (8) is formed in the side wall of the culture pond (1), an air inlet pipe (17) is communicated with the bottom in the mechanism groove (8), an air outlet pipe (18) communicated with the inside of the mechanism groove (8) is further arranged on the inner wall of the culture pond (1), a one-way valve is arranged at the air outlet end of the air outlet pipe (18), a rotating wheel (9) is rotatably connected to the bottom in the mechanism groove (8), a plurality of centrifugal blades (10) are fixedly connected to the side wall of the rotating wheel (9), the rotating wheel (9) is connected with a threaded cylinder (13) through a one-way bearing (15), a screw (12) is connected to the inner thread of the threaded cylinder (13), and a driving device for driving the screw rod (12) to move up and down is arranged in the mechanism groove (8).
2. A photosynthetic microorganism culture apparatus as claimed in claim 1, wherein the driving device comprises a transparent cylinder (6) fixedly arranged at the top in the mechanism groove (8), the transparent cylinder (6) is provided with a mixture of silver bromide and copper oxide, the inner wall of the mechanism groove (8) is provided with a light hole (5), a glass plate (14) is embedded in the light hole (5), the side wall of the threaded cylinder (13) is fixedly connected with a light screen (16) through a plurality of connecting rods, a piston (7) is slidably connected in the transparent cylinder (6) in a sealing manner, a mandril (11) is fixedly connected at the lower end of the piston (7), and the mandril (11) is fixedly connected with the screw (12).
3. A photosynthetic microorganism culture apparatus according to claim 1, characterized in that the ejector rod (11) penetrates through the transparent cylinder (6) in a sealing manner, the transparent cylinder (6) is communicated with an air suction pipe (19) and an air exhaust pipe (20), check valves are installed in the air suction pipe (19) and the air exhaust pipe (20), and the air outlet end of the air exhaust pipe (20) is arranged above the liquid level of the culture pond (1).
4. A photosynthetic microorganism culture apparatus according to claim 2, characterized in that the light-shielding plate (16) is an arc-shaped light-opaque plate, and the area of the forward projection of the light-shielding plate (16) is larger than the area of the glass plate (14).
5. A photosynthetic microorganism culture device according to claim 2, characterized in that one end of the connecting rod is fixedly connected to the side wall of the light screen (16) and the other end of the connecting rod is fixedly connected to the side wall of the threaded cylinder (13).
CN202210034684.1A 2022-01-13 2022-01-13 Photosynthetic microorganism culture apparatus Active CN114410425B (en)

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Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020080200A (en) * 2001-04-12 2002-10-23 김명운 Oxygen generator using photosynthetic microorganisms
TW200610820A (en) * 2004-09-21 2006-04-01 Chao-Hui Lu Plant algae and microbial photosynthesis system and method
CN201406420Y (en) * 2008-10-07 2010-02-17 冯义华 Photosynthetic microbe reactor
JP2012080865A (en) * 2010-10-13 2012-04-26 Toshiro Sekine Method and apparatus for culturing photosynthetic microorganism
CN103184138A (en) * 2011-12-27 2013-07-03 新奥科技发展有限公司 Spectral reactor, and photosynthetic microbe culture apparatus
US20140147915A1 (en) * 2011-07-27 2014-05-29 Yoshiaki Matsuzawa Culture device
CN104328044A (en) * 2014-10-31 2015-02-04 中国科学院大连化学物理研究所 Illumination-carbon dioxide united-regulation and control photo-bioreactor
KR101856678B1 (en) * 2018-03-08 2018-05-14 바이오시스템이엔지(주) Photobioreactor
CN110257221A (en) * 2019-06-24 2019-09-20 王晨 A kind of microalgae bioreactor purifying air and the sunshading board based on the microalgae bioreactor
CN110946027A (en) * 2019-12-09 2020-04-03 邵智勇 Cultivation planting device for urban ecological landscape plants
CN111676126A (en) * 2020-06-28 2020-09-18 烟台大学 Airlift photobioreactor for microalgae culture
CN112075231A (en) * 2020-09-15 2020-12-15 石春林 Light-operated lift adjustment type plant light filling equipment
CN112111377A (en) * 2020-09-30 2020-12-22 赵萍 Constant temperature box capable of automatically removing condensed water
CN112438146A (en) * 2020-10-14 2021-03-05 孙义虎 Plant incubator for biological detection
CN215162619U (en) * 2021-06-28 2021-12-14 北京鑫泽生祥泰科贸有限公司 Self-cleaning microalgae culture equipment

Patent Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020080200A (en) * 2001-04-12 2002-10-23 김명운 Oxygen generator using photosynthetic microorganisms
TW200610820A (en) * 2004-09-21 2006-04-01 Chao-Hui Lu Plant algae and microbial photosynthesis system and method
CN201406420Y (en) * 2008-10-07 2010-02-17 冯义华 Photosynthetic microbe reactor
JP2012080865A (en) * 2010-10-13 2012-04-26 Toshiro Sekine Method and apparatus for culturing photosynthetic microorganism
US20140147915A1 (en) * 2011-07-27 2014-05-29 Yoshiaki Matsuzawa Culture device
CN103184138A (en) * 2011-12-27 2013-07-03 新奥科技发展有限公司 Spectral reactor, and photosynthetic microbe culture apparatus
CN104328044A (en) * 2014-10-31 2015-02-04 中国科学院大连化学物理研究所 Illumination-carbon dioxide united-regulation and control photo-bioreactor
KR101856678B1 (en) * 2018-03-08 2018-05-14 바이오시스템이엔지(주) Photobioreactor
CN110257221A (en) * 2019-06-24 2019-09-20 王晨 A kind of microalgae bioreactor purifying air and the sunshading board based on the microalgae bioreactor
CN110946027A (en) * 2019-12-09 2020-04-03 邵智勇 Cultivation planting device for urban ecological landscape plants
CN111676126A (en) * 2020-06-28 2020-09-18 烟台大学 Airlift photobioreactor for microalgae culture
CN112075231A (en) * 2020-09-15 2020-12-15 石春林 Light-operated lift adjustment type plant light filling equipment
CN112111377A (en) * 2020-09-30 2020-12-22 赵萍 Constant temperature box capable of automatically removing condensed water
CN112438146A (en) * 2020-10-14 2021-03-05 孙义虎 Plant incubator for biological detection
CN215162619U (en) * 2021-06-28 2021-12-14 北京鑫泽生祥泰科贸有限公司 Self-cleaning microalgae culture equipment

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