JP2012080865A - Method and apparatus for culturing photosynthetic microorganism - Google Patents

Method and apparatus for culturing photosynthetic microorganism Download PDF

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JP2012080865A
JP2012080865A JP2010244627A JP2010244627A JP2012080865A JP 2012080865 A JP2012080865 A JP 2012080865A JP 2010244627 A JP2010244627 A JP 2010244627A JP 2010244627 A JP2010244627 A JP 2010244627A JP 2012080865 A JP2012080865 A JP 2012080865A
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Toshiro Sekine
敏朗 関根
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Abstract

PROBLEM TO BE SOLVED: To provide a method and apparatus of a vertically arranged transparent water tank for culturing to proliferate photosynthetic microorganisms healthfully in which animalcules such as water flea, rotifer and the like easily preying on photosynthetic microorganisms are inhibited to invade and proliferate.SOLUTION: The method for culturing photosynthetic microorganisms uses a culture apparatus equipped with a transparent water tank 1 made of light-transmissible material and a floating lid 2 installed in the water tank 1 for proliferating photosynthetic microorganisms by irradiating the contained photosynthetic microorganism suspension with sunlight. (A), Under a light condition, the photosynthetic microorganism suspension is led above the floating lid 2 to be irradiated with light, and (B), under a dark condition, the photosynthetic microorganism suspension is not led above the floating lid 2 so that the animalcules, such as water flea, rotifer and the like preying on photosynthetic microorganisms under an anaerobic condition, are inhibited to proliferate.

Description

発明の詳細な説明Detailed Description of the Invention

本発明は光合成細菌、微細藻類等光合成微生物を培養するための方法及び装置に関する。The present invention relates to a method and apparatus for culturing photosynthetic microorganisms such as photosynthetic bacteria and microalgae.

一般に屋外で太陽光を利用した光合成微生物の培養方式には、微細藻類の培養において見られるように、（１）浅い平面池で攪拌しながら培養するオープンポンド方式、（２）ループ状に設置された直径２０ｃｍ程度の透明チューブ内を循環させながら培養するチュ−ブラ方式（３）直径およそ１ｍ程度、水深およそ２ｍの上部開放透明水槽内で攪拌しながら培養する立設透明水槽方式等がある。
これら屋外培養方式では、光合成微生物を捕食するミジンコ，ワムシ等微小動物の侵入とその繁殖を防ぐことが困難であり、これら捕食生物によって藻類が全滅することがあるという問題点もある。このため、本発明者は、特許第３８４４３６５号（微細藻類培養装置）を考案し、夜間空気との接触を断つことによりオープンポンド方式における捕食生物発生の問題点を解決した。
立設透明水槽方式はオープンポンド方式に比べて設置面積あたりの受光面積が大きいので、設置面積当たりの収穫量が大きいという利点がある。本発明者は、立設透明水槽方式で夜間浮蓋をして空気との接触を断って、捕食生物発生を防止したが、昼間太陽光受光のため浮蓋を取り外し、夜間浮蓋を設置するという労力は多大であった。また、透明材質で作った浮蓋を一日中設置しておくことで捕食生物発生を防止したが、夏季に水温が過度に上昇し微細藻類が死滅した。
特許第３８４４３６５号公報 In general, the photosynthetic microorganism culture method using sunlight outdoors is, as seen in the culture of microalgae, (1) an open pond method in which the culture is performed with stirring in a shallow flat pond, and (2) a loop-like culture. Tubular system for culturing while circulating in a transparent tube having a diameter of about 20 cm (3) There is a standing transparent water tank system for culturing while stirring in an open top transparent water tank having a diameter of about 1 m and a water depth of about 2 m.
In these outdoor culture systems, it is difficult to prevent the invasion and reproduction of minute animals such as daphnia and rotifers that prey on photosynthetic microorganisms, and there is also a problem that algae may be annihilated by these predatory organisms. For this reason, this inventor devised patent 3844365 (microalgae culture apparatus), and solved the problem of predatory organism generation in the open pound system by cutting off contact with air at night.
The standing transparent water tank method has an advantage of a large yield per installation area because it has a larger light receiving area per installation area than the open pound method. The present inventor made a floating in the standing water tank system at night and refused contact with air to prevent predatory organisms, but removed the float for receiving sunlight in the daytime and installed the night float. That effort was tremendous. In addition, the floating lid made of transparent material was installed all day to prevent predatory organisms, but the water temperature rose excessively in summer and the microalgae died.
Japanese Patent No. 3844365

そこで本発明は、上記の点に鑑みなされたもので、光合成微生物を捕食するミジンコ，ワムシ等微小動物の侵入とその繁殖を簡単に防げ、健全に光合成微生物を増殖させる事が可能な、立設透明水槽方式の培養方法及び装置を提供することを目的とするものである。Therefore, the present invention has been made in view of the above points, and can easily prevent the invasion and breeding of minute animals such as daphnids and rotifers that prey on the photosynthetic microorganisms, and can establish the photosynthetic microorganisms in a healthy manner. It is an object of the present invention to provide a transparent water tank type culture method and apparatus.

本発明は、第１に、収容した光合成微生物懸濁液に太陽光を照射し、光合成微生物を増殖させるための、光透過材質で形成された透明水槽、及び該透明水槽内に設置された浮蓋を備える培養装置を用い、（Ａ）明条件下では浮蓋の上方に光合成微生物懸濁液を導き、光を照射し、（Ｂ）暗条件下浮蓋の下に光合成微生物懸濁液を収納し、嫌気条件下光合成微生物を捕食するミジンコ，ワムシ等微小動物の増殖を抑制し、光合成微生物を培養することを特徴とする光合成微生物の培養方法であり、第２に（ア）収容した光合成微生物懸濁液に太陽光を照射し、光合成微生物を増殖させるための、光透過材質で形成された透明水槽、（イ）該透明水槽水面を覆う浮蓋、及び（ウ）該浮蓋の上方に光合成微生物懸濁液を導く液移動機構を備えることを特徴とする光合成微生物の培養装置である。First, the present invention provides a transparent water tank formed of a light-transmitting material for irradiating sunlight to a stored photosynthetic microorganism suspension to propagate the photosynthetic microorganisms, and a floating tank installed in the transparent water tank. Using a culture device equipped with a lid, (A) Under light conditions, the photosynthetic microorganism suspension is guided above the float, irradiated with light, and (B) The photosynthetic microorganism suspension is stored under the float under dark conditions. And a method for culturing photosynthetic microorganisms, which suppresses the growth of small animals such as daphnia and rotifers that prey on the photosynthetic microorganisms under anaerobic conditions, and secondly (a) the photosynthetic microorganisms contained A transparent water tank made of a light-transmitting material for irradiating the suspension with sunlight and allowing the photosynthetic microorganisms to grow, (a) a floating cover that covers the surface of the transparent water tank, and (c) above the floating cover Provide a liquid transfer mechanism to guide the photosynthetic microorganism suspension Is a culture apparatus photosynthetic microorganisms, characterized.

以下、図面を参照しつつ本発明の一実施形態について詳細に説明する。図１乃至図３は本発明の装置の一実施例を示す図面であり、図１は平面図、図２及び図３はＡ−Ａ縦断面図である。本装置は、光透過材質で形成された円筒形の透明水槽１内に浮かせる浮蓋２からなり、透明水槽１側面上方には浮蓋２の浮上を阻止する着脱可能な留め具３、４、５、６が設けられている。浮蓋２の中央には浮蓋２を貫通して、浮蓋２上方と下方を連絡する管７が設けられている。管７内の下端のやや上方には通気管１１が開口している。通気管１１は、エアストーン１２及び開閉弁１０を備え、ブロワー８に連絡されている。透明水槽１の底部には、エアストーン１３が備えられ、通気管９を経てブロワー８に連絡されている。透明水槽１側面には、開閉弁１４を備えた収穫管１５が設けられている。
次に微細藻類を培養する際の、本装置の運転方法について述べる。
図２のように、昼間、浮蓋２は留め具３、４、５、６によって浮上を阻止され、水面下にある。ブロワー８を作動させエアストーン１２及びエアストーン１３から空気を送る。管７ではエアリフト効果によって上昇流が生じ、浮蓋２の周囲に矢印の方向の旋回流が生じる。浮蓋２の下方にも、エアストーン１３からの通気によって、矢印の方向の旋回流が生じる。これによって微細藻類懸濁液（以後懸濁液と略す）は攪拌されながら、水面、透明水槽１側面から太陽光の照射を受け、微細藻類はこの光を吸収して増殖する。
図３のように、日の入りになれば、ブロワー８を止め、開閉弁１４を開け、懸濁液を収穫する。水面は下降する。浮蓋２が留め具３、４、５、６から少し離れるまで水面が下降したら開閉弁１４を閉める。この様にして、浮蓋２下方に格納された懸濁液は外気からの空気の供給がほとんどなく各微生物の呼吸による酸素消費により次第に嫌気状態となり、ワムシやミジンコなど光合成微生物を捕食する微小動物の生存や増殖が制限される。
翌朝には、前日の収穫量に相当する培養液を投入し、ブロワー８を作動させ、前日と同様の昼間の培養を行う。
上記の操作を毎日繰り返しながら培養を続けると、ワムシやミジンコなど微小動物による微細藻類の捕食が制限され、微細藻類の培養を安定的かつ効率的に行なえる。また、昼間懸濁液は大気と接触するので、水蒸発が生じ、懸濁液は冷却されるので、高温期の過熱による微細藻類の死滅を防ぎ、微細藻類を健全に増殖させる事が出来る。
図４及び図５に示した装置は、本発明の別の実施形態を示す縦断面図である。図４が図２に、図５が図３に相当する。本態様では、管７の上端に有孔管１８を設け、その内部に球状の浮遊体２１を備えている点が、図１乃至図３に示した実施形態と異なる。昼間（図４）、浮遊体２１は、有孔管１８内上部に位置し、矢印の様な旋回流が生じる。夜間（図５）は、浮遊体１８は、管７内水面に位置し、気液接触面積がより小さくなり、ワムシやミジンコなど微小動物による生存や増殖がより制限され微細藻類の培養をより安定的かつより効率的に行なえる。
図６及び図７に示した装置は、本発明の別の実施形態を示す縦断面図である。図６が図４に、図７が図５に相当する。本装置は、光透過材質で形成された円筒形の透明水槽１内に気体溜り１６が設けられている。気体溜り１６は上壁面がほぼ水平で、側面は透明水槽１の側面に並行に下方にのび、側面の下端が透明水槽１の底面のやや上方に位置して設けられている。上壁面のほぼ中央に上壁面を貫通して、上壁面上部と気体溜り１６内下方を連絡する管１７が設けられている。管１７の下端は気体溜り１６側面の下端よりもやや上方に位置させてある。
気体溜り１６の上部には、気体溜り１６内に空気等気体を圧入したり排気したりするための管２２を開口させてある。管２２はブロワー８に連絡してある。
朝になり（図６）、ブロワー８を作動させ、空気を気体溜り１６内に圧入すると、水面は上昇し、浮蓋２も上昇するがやがて留め具３、４、５、６でとめられる。水面はさらに上昇し浮蓋２の上壁面から５ｃｍ程度の高さまで上昇する。他方、気体溜り１６内の水面は徐々に下降し、管１７の下端に達し、さらに水面は水の表面張力により管１７の下端よりやや下方まで下降し、やがて空気は管１７の下端から管１７内に一気に溢れ、管１７内に空気層ｇを形成する。この空気層ｇが一気に上昇することにより、透明水槽１内に矢印の方向の旋回流が生じる。空気の圧入の継続により、前記の噴出が一定の周期で間欠的に繰り返される。前述のように、この噴出により、透明水槽１内には循環流が形成され、懸濁液が撹拌される。同時に管７ではエアリフト効果によって上昇流が生じ、浮蓋２の周囲に矢印の方向の旋回流が生じる。このように撹拌された状態で懸濁液は光の照射を受け、液中の微細藻類は光を吸収し増殖する。
日の入りになれば、ブロワー８を止める。気体溜り１６内の空気は管２２、ブロワー８を経て、大気へと逃げる。これに伴い、浮蓋２が留め具３、４、５、６から少し離れる位置まで水面が下降する（図７）。一晩この状態を続け、前記と同様にワムシやミジンコなど光合成微生物を捕食する微小動物の生存や増殖が制限される。
管１７における噴出に関して、噴出は気体圧入速度に関係なく、１回の噴出規模はほぼ一定であり、１回の噴出よる攪拌効果は一定である。このため気体圧入速度を小さく設定しても効果的な攪拌をすることが可能で、電力費の低下につながる。 さらに、管１７の口径を一定とすると、噴出の際、気体溜り１６の横断面積が大きいほど、また管１７下端から水面までの距離が大きいほど、管１７内に流入する空気の量が多く、その結果管１７内に形成される空気層ｇの容積が大きくなり、噴出が激しくなる。また、管１７と気体溜り１６の横断面積の比は、１：３０から１：５０程度が適当である。また、気体溜り１６の実容量は水面を浮蓋２の上壁面から３ｃｍ〜５ｃｍ程度の高さまで上昇させるに足る容量にする。本態様では、ブロワー８のオン−オフだけで攪拌と水面の高さの移動を同時にできる利点がある。前述の懸濁液の収穫と培養液の投入の操作を毎日行う必要がなく、省力的である。
図８に示した装置は、本発明の別の実施形態を示す縦断面図であり、図２に相当する。本装置は、浮蓋２の下壁面を利用した気体溜り１６を備える点が図２の装置と異なる。気体溜り１６は排気のための管２０及び開閉弁１９を備えている。エアストーン１３からの微細気泡は透明水槽１内を攪拌し、上昇し、気体溜り１６内気層１７に流入する。これによって前述のように、管７を介した間欠的噴出が生じ、浮蓋２の周囲に旋回流が生じる。夜間は開閉弁１９を開け、気体溜り２内の空気を排気しておく。本態様では、気体溜り１６の側壁を設ける必要があるが、図２におけるエアストーン１２が必要なくなる。通気動力が低下する利点がある。
図９に示した装置は、本発明の別の実施形態を示す縦断面図であり、図２に相当する。本装置は、図２における留め具３、４、５、６がない点が図２の装置と異なる。昼間、管７を介したエアリフト効果によって、浮蓋２下方の懸濁液が浮蓋２の上壁面上に送られ、上壁面を端部に向かって流下し、透明水槽１内に戻る。この間に太陽光の照射を受ける。夜間はブロワー８を停止しておく。本態様では、図２における留め具３、４、５、６がなく施設費用が低下するが、浮蓋２の上壁面上の水深が極めて小さく、ここで微細藻類に利用される太陽光が少ないという欠点がある。水蒸発は行われるので、高温期の過熱による微細藻類の死滅を防ぎ、微細藻類を健全に増殖させる事が出来る。
微細藻類の商業的生産では、この装置を多数連結して用いる。前述の大きさの装置であれば、２人で移動することも可能である。工場でこの装置を大量生産して、現場で短期間で設置する事が出来る。
また、本発明は、微細藻類以外の光合成微生物、例えば、光合成細菌の培養にも用いる事が出来る事は言うまでもない。Hereinafter, an embodiment of the present invention will be described in detail with reference to the drawings. 1 to 3 are drawings showing an embodiment of the apparatus of the present invention. FIG. 1 is a plan view, and FIGS. 2 and 3 are AA longitudinal sectional views. The apparatus comprises a floating lid 2 that floats in a cylindrical transparent water tank 1 made of a light-transmitting material, and detachable fasteners 3, 4 that prevent the floating lid 2 from floating above the side of the transparent water tank 1. 5 and 6 are provided. A tube 7 is provided in the center of the float 2 so as to penetrate the float 2 and connect the upper and lower sides of the float 2. A vent pipe 11 is opened slightly above the lower end in the pipe 7. The vent pipe 11 includes an air stone 12 and an on-off valve 10 and is in communication with the blower 8. An air stone 13 is provided at the bottom of the transparent water tank 1 and communicates with the blower 8 through a vent pipe 9. On the side surface of the transparent water tank 1, a harvesting pipe 15 having an on-off valve 14 is provided.
Next, the operation method of this apparatus when culturing microalgae will be described.
As shown in FIG. 2, during the daytime, the floating lid 2 is prevented from rising by the fasteners 3, 4, 5, and 6 and is below the surface of the water. The blower 8 is operated to send air from the air stone 12 and the air stone 13. In the pipe 7, an upward flow is generated by the air lift effect, and a swirling flow in the direction of the arrow is generated around the floating lid 2. A swirling flow in the direction of the arrow is also generated below the floating lid 2 by the ventilation from the air stone 13. As a result, the microalgae suspension (hereinafter abbreviated as suspension) is irradiated with sunlight from the water surface and the side of the transparent water tank 1 while being stirred, and the microalgae absorbs this light and grows.
As shown in FIG. 3, when sunset occurs, the blower 8 is stopped, the on-off valve 14 is opened, and the suspension is harvested. The water surface descends. When the water surface descends until the float 2 is slightly separated from the fasteners 3, 4, 5, 6, the on-off valve 14 is closed. In this way, the suspension stored under the floating lid 2 is hardly supplied with air from the outside air and becomes gradually anaerobic due to oxygen consumption by the respiration of each microorganism, and is a micro animal that prey on photosynthetic microorganisms such as rotifers and daphnia Survival and proliferation are limited.
On the next morning, the culture solution corresponding to the harvest amount of the previous day is added, the blower 8 is operated, and the same daytime culture is performed as the previous day.
If the culture is continued while repeating the above operation every day, predation of microalgae by tiny animals such as rotifers and daphnia is restricted, and microalgae can be cultured stably and efficiently. In addition, since the daytime suspension comes into contact with the atmosphere, water evaporation occurs, and the suspension is cooled. Therefore, the microalgae can be prevented from being killed by overheating in the high temperature period, and the microalgae can be grown healthy.
The apparatus shown in FIGS. 4 and 5 is a longitudinal sectional view showing another embodiment of the present invention. 4 corresponds to FIG. 2, and FIG. 5 corresponds to FIG. This embodiment is different from the embodiment shown in FIGS. 1 to 3 in that a perforated tube 18 is provided at the upper end of the tube 7 and a spherical floating body 21 is provided therein. During the daytime (FIG. 4), the floating body 21 is located in the upper part of the perforated pipe 18, and a swirling flow like an arrow is generated. At night (Fig. 5), the floating body 18 is located on the water surface in the tube 7, and the gas-liquid contact area is smaller, and the survival and growth of micro-animals such as rotifers and daphnids are more restricted, and the culture of microalgae is more stable. And more efficient.
The apparatus shown in FIGS. 6 and 7 is a longitudinal sectional view showing another embodiment of the present invention. 6 corresponds to FIG. 4, and FIG. 7 corresponds to FIG. In this apparatus, a gas reservoir 16 is provided in a cylindrical transparent water tank 1 made of a light transmitting material. The gas reservoir 16 is provided such that the upper wall surface is substantially horizontal, the side surface extends downward in parallel with the side surface of the transparent water tank 1, and the lower end of the side surface is positioned slightly above the bottom surface of the transparent water tank 1. A pipe 17 is provided in the middle of the upper wall surface so as to penetrate the upper wall surface and communicate with the upper portion of the upper wall surface and the lower side in the gas reservoir 16. The lower end of the pipe 17 is positioned slightly above the lower end of the side surface of the gas reservoir 16.
In the upper part of the gas reservoir 16, a tube 22 for opening and exhausting a gas such as air into the gas reservoir 16 is opened. Tube 22 communicates with blower 8.
In the morning (FIG. 6), when the blower 8 is operated and air is pressed into the gas reservoir 16, the water surface rises and the float 2 also rises, but is eventually stopped by the fasteners 3, 4, 5, and 6. The water surface rises further and rises to a height of about 5 cm from the upper wall surface of the floating lid 2. On the other hand, the water surface in the gas reservoir 16 gradually descends to reach the lower end of the tube 17, and further the water surface descends slightly below the lower end of the tube 17 due to the surface tension of the water. The air layer g overflows inside and forms an air layer g in the tube 17. As the air layer g rises at once, a swirling flow in the direction of the arrow is generated in the transparent water tank 1. Due to the continuation of the press-fitting of air, the ejection is intermittently repeated at a constant cycle. As described above, by this ejection, a circulating flow is formed in the transparent water tank 1, and the suspension is stirred. At the same time, an upward flow is generated in the pipe 7 by the air lift effect, and a swirling flow in the direction of the arrow is generated around the floating lid 2. In this state of stirring, the suspension is irradiated with light, and the microalgae in the liquid absorb light and grow.
Stop the blower 8 at sunset. The air in the gas reservoir 16 escapes to the atmosphere through the pipe 22 and the blower 8. Along with this, the water surface descends to a position where the floating lid 2 is slightly away from the fasteners 3, 4, 5, 6 (FIG. 7). This state is continued overnight, and the survival and growth of micro-animals that prey on photosynthetic microorganisms such as rotifers and daphnids are restricted as described above.
Regarding the ejection in the pipe 17, the ejection size of the ejection is almost constant regardless of the gas injection speed, and the stirring effect by the ejection is constant. For this reason, even if the gas injection speed is set to a small value, effective stirring can be performed, which leads to a reduction in power cost. Furthermore, if the diameter of the pipe 17 is constant, the greater the cross-sectional area of the gas reservoir 16 and the greater the distance from the lower end of the pipe 17 to the water surface, the greater the amount of air flowing into the pipe 17 during ejection. As a result, the volume of the air layer g formed in the pipe 17 becomes large, and the ejection becomes intense. The ratio of the cross-sectional area between the tube 17 and the gas reservoir 16 is suitably about 1:30 to 1:50. The actual capacity of the gas reservoir 16 is set to a capacity sufficient to raise the water surface from the upper wall surface of the floating lid 2 to a height of about 3 cm to 5 cm. In this aspect, there is an advantage that the stirring and the movement of the height of the water surface can be simultaneously performed only by turning on / off the blower 8. The above-described operations of harvesting the suspension and introducing the culture solution do not need to be performed every day, which is labor-saving.
The apparatus shown in FIG. 8 is a longitudinal sectional view showing another embodiment of the present invention, and corresponds to FIG. This apparatus is different from the apparatus of FIG. 2 in that a gas reservoir 16 using the lower wall surface of the floating lid 2 is provided. The gas reservoir 16 includes a pipe 20 for exhaust and an opening / closing valve 19. The fine bubbles from the air stone 13 are stirred in the transparent water tank 1 and rise to flow into the gas reservoir 16 inside air layer 17. As a result, intermittent ejection occurs through the pipe 7 as described above, and a swirling flow is generated around the floating lid 2. At night, the on-off valve 19 is opened and the air in the gas reservoir 2 is exhausted. In this embodiment, it is necessary to provide the side wall of the gas reservoir 16, but the air stone 12 in FIG. There is an advantage that the ventilation power is reduced.
The apparatus shown in FIG. 9 is a longitudinal sectional view showing another embodiment of the present invention, and corresponds to FIG. This apparatus differs from the apparatus of FIG. 2 in that there are no fasteners 3, 4, 5, 6 in FIG. During the daytime, the suspension below the floating lid 2 is sent onto the upper wall surface of the floating lid 2 by the air lift effect through the pipe 7, flows down the upper wall surface toward the end, and returns to the transparent water tank 1. During this time, it receives sunlight. The blower 8 is stopped at night. In this embodiment, there is no fastener 3, 4, 5, 6 in FIG. 2, and the facility cost is reduced, but the water depth on the upper wall surface of the floating lid 2 is extremely small, and there is little sunlight utilized for microalgae here. There is a drawback. Since water evaporation is performed, the microalgae can be prevented from dying due to overheating in the high temperature period, and the microalgae can be propagated healthy.
In commercial production of microalgae, a large number of these devices are connected and used. If it is the apparatus of the above-mentioned size, it is also possible for two people to move. This device can be mass-produced at the factory and installed in the field in a short time.
Moreover, it cannot be overemphasized that this invention can be used also for culture | cultivation of photosynthetic microorganisms other than a micro algae, for example, photosynthetic bacteria.

発明の効果The invention's effect

本発明の効果として、まとめれば、下記の事項が挙げられる。
（１）装置設置面積当たりの増殖量が大きいので、設置面積を節約できる。
養豚場の廃水処理を兼ねて設置する場合、養豚場は広い敷地を持っていない所も多いので、設置面積が節約できる本発明は極めて重宝である。
（２）高温期の過熱による微細藻類の死滅を防ぎ、微細藻類を健全に増殖させる事が出来る。
（３）ワムシやミジンコなど微小動物による生存や増殖が制限され微細藻類の培養を安定的かつより効率的に行なえる。懸濁液を収穫し、収穫した分だけ、水や培養液を投入して、培養を継続していく、いはゆる、連続培養が可能であり、操作が簡単で労力が少ない。
（４）工場で大量生産して、現場で短期間で設置する事が出来る。The effects of the present invention are summarized as follows.
(1) Since the proliferation amount per apparatus installation area is large, the installation area can be saved.
In the case where the pig farm is installed also for wastewater treatment, the pig farm does not have a large site, so the present invention that can save the installation area is extremely useful.
(2) Microalgae can be prevented from dying due to overheating in the high temperature period, and the microalgae can be propagated healthy.
(3) Survival and growth by micro-animals such as rotifers and daphnia are restricted, and microalgae can be cultured stably and more efficiently. Suspension is harvested, water and culture solution are added to the harvested amount, and the culture is continued. In other words, continuous culture is possible, and the operation is simple and labor is small.
(4) It can be mass-produced at a factory and installed in a short period of time.

本発明の一実施形態を示す平面図である。It is a top view which shows one Embodiment of this invention. 図１におけるＡ−Ａ縦断面図である。It is an AA longitudinal cross-sectional view in FIG. 図１におけるＡ−Ａ縦断面図である。It is an AA longitudinal cross-sectional view in FIG. 別の実施形態を示す縦断面図である。It is a longitudinal cross-sectional view which shows another embodiment. 別の実施形態を示す縦断面図である。It is a longitudinal cross-sectional view which shows another embodiment. 別の実施形態を示す縦断面図である。It is a longitudinal cross-sectional view which shows another embodiment. 別の実施形態を示す縦断面図である。It is a longitudinal cross-sectional view which shows another embodiment. 別の実施形態を示す縦断面図である。It is a longitudinal cross-sectional view which shows another embodiment. 別の実施形態を示す縦断面図である。It is a longitudinal cross-sectional view which shows another embodiment.

１は透明水槽、２は浮蓋、３は留め具、４は留め具、５は留め具、６は留め具、７は管、８はブロワー、９は通気管、１０は開閉弁、１１は通気管、１２はエアストーン、１３はエアストーン、１４は開閉弁、１５は収穫管、１６は気体溜り、１７は空気層管、１８は有孔管、１９は開閉弁、２０は管、２１は浮遊体、２２は管、ｇは空気層、実線矢印は微細藻類懸濁液の流れの方向を示す。1 is a transparent water tank, 2 is a floating lid, 3 is a fastener, 4 is a fastener, 5 is a fastener, 6 is a fastener, 7 is a pipe, 8 is a blower, 9 is a vent pipe, 10 is an on-off valve, 11 is Ventilation pipe, 12 is an air stone, 13 is an air stone, 14 is an open / close valve, 15 is a harvest pipe, 16 is a gas reservoir, 17 is an air layer pipe, 18 is a perforated pipe, 19 is an open / close valve, 20 is a pipe, 21 Is a floating body, 22 is a tube, g is an air layer, and a solid line arrow indicates the flow direction of the microalgae suspension.

Claims (6)

収容した光合成微生物懸濁液に太陽光を照射し、光合成微生物を増殖させるための、光透過材質で形成された透明水槽、及び該透明水槽内に設置された浮蓋を備える培養装置を用い、（Ａ）明条件下では浮蓋の上方に光合成微生物懸濁液を導き、光を照射し、（Ｂ）暗条件下浮蓋の下に光合成微生物懸濁液を収納し、嫌気条件下光合成微生物を捕食するミジンコ，ワムシ等微小動物の増殖を抑制し、光合成微生物を培養することを特徴とする光合成微生物の培養方法。Using a culture apparatus provided with a transparent water tank formed of a light-transmitting material and a floating lid installed in the transparent water tank for irradiating the photosynthetic microorganism suspension accommodated with sunlight to grow the photosynthetic microorganisms, (A) Under the light conditions, the photosynthetic microorganism suspension is guided above the buoyant lid and irradiated with light. (B) The photosynthetic microorganism suspension is stored under the levitation under the dark condition. A method for cultivating photosynthetic microorganisms, characterized by suppressing the growth of predatory daphnia, rotifers and other small animals and culturing photosynthetic microorganisms. 上記（Ａ）工程では、浮蓋の浮上を阻止し、水位を上昇させ、上記（Ｂ）工程では、水位を下降させることを特徴とする請求項１記載の光合成微生物の培養方法。The method for cultivating photosynthetic microorganisms according to claim 1, wherein in the step (A), the float is prevented from rising and the water level is raised, and in the step (B), the water level is lowered. （ア）収容した光合成微生物懸濁液に太陽光を照射し、光合成微生物を増殖させるための、光透過材質で形成された透明水槽、（イ）該透明水槽水面を覆う浮蓋、及び（ウ）該浮蓋の上方に光合成微生物懸濁液を導く液移動機構を備えることを特徴とする光合成微生物の培養装置。(A) A transparent aquarium formed of a light-transmitting material for irradiating the stored photosynthetic microorganism suspension with sunlight and allowing the photosynthetic microorganisms to grow, (a) a floating lid covering the water surface of the transparent aquarium, and (c) ) An apparatus for culturing photosynthetic microorganisms, comprising a liquid movement mechanism for guiding a photosynthetic microorganism suspension above the floating lid. 前記透明水槽内に浮蓋の上昇阻止具が装着され、これによって前記液移動機構が構成されていることを特徴とする請求項３記載の光合成微生物の培養装置。4. The apparatus for cultivating photosynthetic microorganisms according to claim 3, wherein a rising prevention device for a floating lid is mounted in the transparent water tank, whereby the liquid movement mechanism is constituted. 前記浮蓋の上方へ浮蓋下方の懸濁液を送るエアリフトポンプが装着され、これによって前記液移動機構が構成されていることを特徴とする請求項３または請求項４記載の光合成微生物の培養装置。The culture of photosynthetic microorganisms according to claim 3 or 4, wherein an air lift pump for sending a suspension below the buoy is provided above the buoy, and thereby the liquid movement mechanism is configured. apparatus. 前記透明水槽内に通排気機構を備えた気体溜りが装着され、これによって前記液移動機構が構成されていることを特徴とする請求項３または請求項４または請求項５記載の光合成微生物の培養装置。6. The culture of photosynthetic microorganisms according to claim 3, 4 or 5, wherein a gas reservoir having a ventilating mechanism is mounted in the transparent water tank, thereby constituting the liquid moving mechanism. apparatus.
JP2010244627A 2010-10-13 2010-10-13 Method and apparatus for culturing photosynthetic microorganism Pending JP2012080865A (en)

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CN114410425A (en) * 2022-01-13 2022-04-29 冯鸿彬 Photosynthetic microorganism culture apparatus

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JP2017079602A (en) * 2015-10-23 2017-05-18 株式会社デンソー Culture apparatus and culture method of photosynthetic microorganism
CN114410425A (en) * 2022-01-13 2022-04-29 冯鸿彬 Photosynthetic microorganism culture apparatus
CN114410425B (en) * 2022-01-13 2024-02-06 上海知楚仪器有限公司 Photosynthetic microorganism culture apparatus

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